Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

5125838 .. 5128780

Gene Structure 2943 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g09560.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGTTTCAGTTTGTGGTGATAGGGTTCCTTTTGGTACTGGGAGGGGCCTCAGTGTCTGGGGCTGCCTTGGAGAAGTACCAAGTCTATGTTCAAAAGGAATGCAGCTTTACAAGATATCCCAGCATTTGTTTTCAAAGCTTAATGGGGTTGGGTGGTTCTGGTTCTGGGCACCAGGATCCTCAACATGTTGATATAATGTCGGCTCTTGTTAACAAAACCATTTATGAAACCCGGTTGACCTCCTCTGAGTTTTCCAAATTCAGCTCTCAATTATTTGAAGTCCGTGATTCTCAACCCACACACGATTCTGGCAGCAAAGACTACTGTGAAGAGCTAATGACGATGTCCTTGAAGCGCTTAGAACAATCCCTAATAGCTCTCAAGAAATCTCCCTCAAAAAACAAGCAAGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCAAGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGCTCGACCTTTCCAAGCGCATAGGCTATACCTCTCAGTTGGGGAGTAATCTATTAGCTCTCGTCAACCGTGTTTCGAGTAATTCCGATAATCCGAAGAACACAACTCGTGGTAAAAAGGCAGAGATGTTTCCCAAATGGGTTTCGCCGAAGAACAGGAAACTACTTCAGGCAACCACCGTCAAAGCTAACGTGGTGGTTGCGAAAGATGGATCAGGGAACTATAAGACGGTGTCGGAGGCCATTCAGGCAGCTACTGGGGGTCGGTTTGTGATTTATGTAAAGGCTGGAGTTTATAAGGAGAAGATTAAGACTAACAAAGATGGTATTACGTTGATTGGAGATGGACAATATTCCACTATTATTACTGGAGATTCTAGTGTGTCTAAAGGTTCTACCATGCCTGGCTCAGCTACATTTACCGTAACAGGAGATGGATTCATAGCTCGAGATATTGGTTTCCAAAACACAGCCGGCCCCGACGCAGAACAAGCCCTAGCTCTGTACATCTCTTCTGATCACTCCGTTCTCTACAAGTGTAGCATCGTTGGCTACCAAGACACACTCTACGCCCTCGCCCTCCGCCAATTCTACAGAGAATGCGACATCTACGGCAGCATCGACTTCATCTTCGGTAACGCCGCCGCCGTCTTCCAGAGTTGCAATATCGTACTCCGCAGACACAAGGGTTACAATGCCATCCTGGCAAGCGGGAGGTCCGACCCAGGACAAAAGACAGGTTTCTCCGTCCAGAACTGTAGGATCGTGCCCGGGTCGGACTTATCTCCCATTAAACACTCCTATAGTTCGTATCTTGGTAGGCCATGGAAGGACTACGCTAGAGCCGTGGTCATGCAATCCAACATAGATGACGTTATCTCACCCCGAGGGTGGGTTGAGTGGCCTGGCTCCAGCCCCAAGTCTATCTATTTTGCGGAGTTCGCAAATGTAGGATCTGGGGCTGGAGTGAGCCAGAGGGTGCAGTGGCCAGGGTTTCATGTAATTGGAGCTGATGTTGCTGTTGAGTATACTGTTGGTAACTTTATTGCTGGAACTTCATGGCTGCCTTCGACCGGTGTGACTTTTGTTTCTGGCCTCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

537

Amino Acids

58.26

Weight (kDa)

8.93

Isoelectric Point (pI)

37.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	29 - 190	2.3e-22	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	233 - 520	6.9e-116	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	804
AccB1I	GGYRCC	1 cut(s)	168
AccI	GTMKAC	1 cut(s)	1535
AciI	CCGC	8 cut(s)	432, 468, 1090, 1149, 1152, 1183, 1218, 1442
AclWI	GGATC	5 cut(s)	170, 183, 727, 1277, 1468
AcoI	YGGCCR	1 cut(s)	1493
AcsI	RAATTY	1 cut(s)	257
AcuI	CTGAAG	1 cut(s)	659
AfaI	GTAC	4 cut(s)	36, 77, 1013, 1179
AfeI	AGCGCT	1 cut(s)	356
AfiI	CCNNNNNNNGG	3 cut(s)	1398, 1399, 1580
AflIII	ACRYGT	1 cut(s)	184
AgeI	ACCGGT	1 cut(s)	1580
AgsI	TTSAA	4 cut(s)	92, 134, 278, 352
AjnI	CCWGG	6 cut(s)	171, 910, 1209, 1231, 1411, 1495
AjuI	GAANNNNNNNTTGG	2 cut(s)	72, 104
AlwI	GGATC	5 cut(s)	170, 183, 727, 1277, 1468
AlwNI	CAGNNNCTG	2 cut(s)	56, 764
Ama87I	CYCGRG	3 cut(s)	954, 1277, 1392
Aor51HI	AGCGCT	1 cut(s)	356
AoxI	GGCC	8 cut(s)	45, 478, 747, 982, 1328, 1409, 1493, 1600
ApeKI	GCWGC	6 cut(s)	62, 102, 312, 758, 1122, 1570
ApoI	RAATTY	1 cut(s)	257
ArsI	GACNNNNNNTTYG	2 cut(s)	1121, 1153
AsiGI	ACCGGT	1 cut(s)	1580
AspLEI	GCGC	2 cut(s)	357, 528
AspS9I	GGNCC	3 cut(s)	45, 983, 1224
AsuC2I	CCSGG	3 cut(s)	232, 1278, 1279
AsuHPI	GGTGA	2 cut(s)	28, 1380
AvaI	CYCGRG	3 cut(s)	954, 1277, 1392
AvaII	GGWCC	1 cut(s)	1224
BaeGI	GKGCMC	2 cut(s)	171, 1278
BalI	TGGCCA	1 cut(s)	1495
BamHI	GGATCC	1 cut(s)	175
BanI	GGYRCC	1 cut(s)	168
BauI	CACGAG	1 cut(s)	615
BbsI	GAAGAC	1 cut(s)	1150
BbvCI	CCTCAGC	1 cut(s)	482
BbvI	GCAGC	6 cut(s)	49, 114, 324, 770, 1134, 1557
BccI	CCATC	5 cut(s)	710, 824, 845, 935, 1214
BceAI	ACGGC	4 cut(s)	493, 1135, 1139, 1337
BciT130I	CCWGG	6 cut(s)	173, 912, 1211, 1233, 1413, 1497
BclI	TGATCA	1 cut(s)	1024
BcnI	CCSGG	3 cut(s)	232, 1278, 1279
BfaI	CTAG	4 cut(s)	492, 885, 1004, 1347
BfmI	CTRYAG	3 cut(s)	1099, 1264, 1309
BfoI	RGCGCY	1 cut(s)	358
BglI	GCCNNNNNGGC	2 cut(s)	755, 1211
BlpI	GCTNAGC	1 cut(s)	916
Bme1390I	CCNGG	9 cut(s)	173, 232, 912, 1211, 1233, 1278, 1279, 1413, 1497
Bme18I	GGWCC	1 cut(s)	1224
BmeT110I	CYCGRG	3 cut(s)	954, 1277, 1392
BmgT120I	GGNCC	3 cut(s)	45, 983, 1224
BmiI	GGNNCC	6 cut(s)	26, 46, 170, 177, 985, 1417
BmrFI	CCNGG	9 cut(s)	173, 232, 912, 1211, 1233, 1278, 1279, 1413, 1497
BmrI	ACTGGG	2 cut(s)	47, 774
BmsI	GCATC	2 cut(s)	1059, 1134
BmuI	ACTGGG	2 cut(s)	47, 774
BpiI	GAAGAC	1 cut(s)	1150
BpmI	CTGGAG	4 cut(s)	816, 897, 1402, 1491
Bpu10I	CCTNAGC	1 cut(s)	482
Bpu1102I	GCTNAGC	1 cut(s)	916
BpuEI	CTTGAG	1 cut(s)	365
BpuMI	CCSGG	3 cut(s)	232, 1278, 1279
BsaJI	CCNNGG	8 cut(s)	66, 475, 1231, 1277, 1331, 1353, 1393, 1496
BsaWI	WCCGGW	1 cut(s)	1580
BsaXI	ACNNNNNCTCC	2 cut(s)	1506, 1536
Bsc4I	CCNNNNNNNGG	3 cut(s)	1398, 1399, 1580
Bse118I	RCCGGY	2 cut(s)	980, 1580
Bse1I	ACTGG	3 cut(s)	42, 769, 880
BseBI	CCWGG	6 cut(s)	173, 912, 1211, 1233, 1413, 1497
BseDI	CCNNGG	8 cut(s)	66, 475, 1231, 1277, 1331, 1353, 1393, 1496
BseGI	GGATG	1 cut(s)	1206
BseLI	CCNNNNNNNGG	3 cut(s)	1398, 1399, 1580
BseMII	CTCAG	6 cut(s)	63, 237, 496, 520, 557, 930
BseNI	ACTGG	3 cut(s)	42, 769, 880
BseRI	GAGGAG	1 cut(s)	232
BseSI	GKGCMC	2 cut(s)	171, 1278
BseXI	GCAGC	6 cut(s)	49, 114, 324, 770, 1134, 1557
BseYI	CCCAGC	1 cut(s)	119
BsgI	GTGCAG	1 cut(s)	1508
Bsh1285I	CGRYCG	1 cut(s)	1581
BshFI	GGCC	8 cut(s)	47, 480, 749, 984, 1330, 1411, 1495, 1602
BshNI	GGYRCC	1 cut(s)	168
BshTI	ACCGGT	1 cut(s)	1580
BsiEI	CGRYCG	1 cut(s)	1581
BsiHKCI	CYCGRG	3 cut(s)	954, 1277, 1392
BsiSI	CCGG	4 cut(s)	232, 981, 1278, 1581
BslI	CCNNNNNNNGG	3 cut(s)	1398, 1399, 1580
BsmI	GAATGC	2 cut(s)	104, 1112
BsnI	GGCC	8 cut(s)	47, 480, 749, 984, 1330, 1411, 1495, 1602
BsoBI	CYCGRG	3 cut(s)	954, 1277, 1392
Bsp1286I	GDGCHC	2 cut(s)	171, 1278
Bsp1407I	TGTACA	1 cut(s)	1011
Bsp143I	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
Bsp1720I	GCTNAGC	1 cut(s)	916
Bsp19I	CCATGG	1 cut(s)	1331
BspACI	CCGC	8 cut(s)	432, 468, 1090, 1149, 1152, 1183, 1218, 1442
BspANI	GGCC	8 cut(s)	47, 480, 749, 984, 1330, 1411, 1495, 1602
BspCNI	CTCAG	6 cut(s)	62, 238, 495, 519, 556, 929
BspLI	GGNNCC	6 cut(s)	26, 46, 170, 177, 985, 1417
BspPI	GGATC	5 cut(s)	170, 183, 727, 1277, 1468
BspQI	GCTCTTC	1 cut(s)	324
BspT107I	GGYRCC	1 cut(s)	168
BsrFI	RCCGGY	2 cut(s)	980, 1580
BsrGI	TGTACA	1 cut(s)	1011
BsrI	ACTGG	3 cut(s)	42, 769, 880
BssAI	RCCGGY	2 cut(s)	980, 1580
BssECI	CCNNGG	8 cut(s)	66, 475, 1231, 1277, 1331, 1353, 1393, 1496
BssMI	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
BssNAI	GTATAC	1 cut(s)	1536
BssSI	CACGAG	1 cut(s)	615
BssT1I	CCWWGG	2 cut(s)	66, 1331
Bst1107I	GTATAC	1 cut(s)	1536
Bst2BI	CACGAG	1 cut(s)	615
Bst2UI	CCWGG	6 cut(s)	173, 912, 1211, 1233, 1413, 1497
Bst4CI	ACNGT	7 cut(s)	326, 578, 688, 739, 931, 1265, 1540
Bst6I	CTCTTC	2 cut(s)	324, 1024
BstAUI	TGTACA	1 cut(s)	1011
BstC8I	GCNNGC	2 cut(s)	982, 1216
BstDEI	CTNAG	8 cut(s)	49, 246, 358, 425, 482, 506, 543, 916
BstDSI	CCRYGG	3 cut(s)	475, 1331, 1353
BstF5I	GGATG	1 cut(s)	1206
BstH2I	RGCGCY	1 cut(s)	358
BstHHI	GCGC	2 cut(s)	357, 528
BstKTI	GATC	5 cut(s)	178, 722, 1027, 1272, 1463
BstMBI	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
BstMCI	CGRYCG	1 cut(s)	1581
BstMWI	GCNNNNNNNGC	7 cut(s)	492, 755, 998, 1056, 1211, 1448, 1523
BstNI	CCWGG	6 cut(s)	173, 912, 1211, 1233, 1413, 1497
BstNSI	RCATGY	1 cut(s)	188
BstSCI	CCNGG	9 cut(s)	171, 230, 910, 1209, 1231, 1276, 1277, 1411, 1495
BstSFI	CTRYAG	3 cut(s)	1099, 1264, 1309
BstSLI	GKGCMC	2 cut(s)	171, 1278
BstV1I	GCAGC	6 cut(s)	49, 114, 324, 770, 1134, 1557
BstV2I	GAAGAC	1 cut(s)	1150
BstX2I	RGATCY	2 cut(s)	175, 1460
BstYI	RGATCY	2 cut(s)	175, 1460
BstZ17I	GTATAC	1 cut(s)	1536
BsuRI	GGCC	8 cut(s)	47, 480, 749, 984, 1330, 1411, 1495, 1602
BtgI	CCRYGG	3 cut(s)	475, 1331, 1353
BtsCI	GGATG	1 cut(s)	1206
BtsI	GCAGTG	1 cut(s)	1496
BtsIMutI	CAGTG	2 cut(s)	57, 1496
Cac8I	GCNNGC	2 cut(s)	982, 1216
CaiI	CAGNNNCTG	2 cut(s)	56, 764
CfoI	GCGC	2 cut(s)	357, 528
Cfr10I	RCCGGY	2 cut(s)	980, 1580
Cfr13I	GGNCC	3 cut(s)	45, 983, 1224
Cfr9I	CCCGGG	1 cut(s)	1277
CseI	GACGC	1 cut(s)	998
Csp6I	GTAC	4 cut(s)	35, 76, 1012, 1178
CspAI	ACCGGT	1 cut(s)	1580
CviAII	CATG	7 cut(s)	185, 420, 907, 1332, 1360, 1505, 1566
CviQI	GTAC	4 cut(s)	35, 76, 1012, 1178
DdeI	CTNAG	8 cut(s)	49, 246, 358, 425, 482, 506, 543, 916
DpnI	GATC	5 cut(s)	177, 721, 1026, 1271, 1462
DpnII	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
EaeI	YGGCCR	1 cut(s)	1493
Eam1104I	CTCTTC	2 cut(s)	324, 1024
EarI	CTCTTC	2 cut(s)	324, 1024
EciI	GGCGGA	1 cut(s)	1079
Eco130I	CCWWGG	2 cut(s)	66, 1331
Eco32I	GATATC	1 cut(s)	116
Eco47I	GGWCC	1 cut(s)	1224
Eco47III	AGCGCT	1 cut(s)	356
Eco57I	CTGAAG	1 cut(s)	659
Eco88I	CYCGRG	3 cut(s)	954, 1277, 1392
EcoO109I	RGGNCCY	1 cut(s)	45
EcoRII	CCWGG	6 cut(s)	171, 910, 1209, 1231, 1411, 1495
EcoRV	GATATC	1 cut(s)	116
EcoT14I	CCWWGG	2 cut(s)	66, 1331
ErhI	CCWWGG	2 cut(s)	66, 1331
FaeI	CATG	7 cut(s)	188, 423, 910, 1335, 1363, 1508, 1569
FatI	CATG	7 cut(s)	184, 419, 906, 1331, 1359, 1504, 1565
FauI	CCCGC	1 cut(s)	1211
FbaI	TGATCA	1 cut(s)	1024
FblI	GTMKAC	1 cut(s)	1535
FokI	GGATG	1 cut(s)	1193
FspBI	CTAG	4 cut(s)	492, 885, 1004, 1347
GlaI	GCGC	2 cut(s)	356, 527
GsaI	CCCAGC	1 cut(s)	123
GsuI	CTGGAG	4 cut(s)	816, 897, 1402, 1491
HaeII	RGCGCY	1 cut(s)	358
HaeIII	GGCC	8 cut(s)	47, 480, 749, 984, 1330, 1411, 1495, 1602
HapII	CCGG	4 cut(s)	232, 981, 1278, 1581
HgaI	GACGC	1 cut(s)	998
HhaI	GCGC	2 cut(s)	357, 528
Hin1II	CATG	7 cut(s)	188, 423, 910, 1335, 1363, 1508, 1569
Hin6I	GCGC	2 cut(s)	355, 526
HinP1I	GCGC	2 cut(s)	355, 526
HincII	GTYRAC	3 cut(s)	211, 237, 574
HindII	GTYRAC	3 cut(s)	211, 237, 574
HindIII	AAGCTT	1 cut(s)	136
HinfI	GANTC	5 cut(s)	287, 305, 502, 881, 945
HpaI	GTTAAC	1 cut(s)	211
HpaII	CCGG	4 cut(s)	232, 981, 1278, 1581
HphI	GGTGA	2 cut(s)	28, 1380
Hpy166II	GTNNAC	4 cut(s)	211, 237, 574, 1536
Hpy188I	TCNGA	8 cut(s)	247, 499, 595, 603, 745, 1024, 1228, 1285
Hpy188III	TCNNGA	4 cut(s)	382, 956, 1162, 1258
Hpy8I	GTNNAC	4 cut(s)	211, 237, 574, 1536
Hpy99I	CGWCG	1 cut(s)	992
HpyAV	CCTTC	2 cut(s)	1330, 1584
HpyCH4III	ACNGT	7 cut(s)	326, 578, 688, 739, 931, 1265, 1540
HpyCH4IV	ACGT	4 cut(s)	452, 699, 839, 1380
HpyCH4V	TGCA	5 cut(s)	102, 456, 1170, 1363, 1489
HpyF10VI	GCNNNNNNNGC	7 cut(s)	492, 755, 998, 1056, 1211, 1448, 1523
HpyF3I	CTNAG	8 cut(s)	49, 246, 358, 425, 482, 506, 543, 916
HpySE526I	ACGT	4 cut(s)	452, 699, 839, 1380
Hsp92II	CATG	7 cut(s)	188, 423, 910, 1335, 1363, 1508, 1569
HspAI	GCGC	2 cut(s)	355, 526
KroI	GCCGGC	1 cut(s)	980
KroNI	GCCGGC	1 cut(s)	982
Ksp22I	TGATCA	1 cut(s)	1024
KspAI	GTTAAC	1 cut(s)	211
Kzo9I	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
LguI	GCTCTTC	1 cut(s)	324
LmnI	GCTCC	3 cut(s)	500, 1421, 1514
Lsp1109I	GCAGC	6 cut(s)	49, 114, 324, 770, 1134, 1557
LweI	GCATC	2 cut(s)	1059, 1134
MaeI	CTAG	4 cut(s)	492, 885, 1004, 1347
MaeII	ACGT	4 cut(s)	452, 699, 839, 1380
MaeIII	GTNAC	5 cut(s)	931, 1142, 1196, 1544, 1585
MalI	GATC	5 cut(s)	177, 721, 1026, 1271, 1462
MboI	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
MboII	GAAGA	7 cut(s)	341, 616, 670, 823, 1011, 1129, 1150
MflI	RGATCY	2 cut(s)	175, 1460
MhlI	GDGCHC	2 cut(s)	171, 1278
MlsI	TGGCCA	1 cut(s)	1495
MluCI	AATT	5 cut(s)	257, 269, 589, 1094, 1509
MluNI	TGGCCA	1 cut(s)	1495
MmeI	TCCRAC	4 cut(s)	723, 1251, 1263, 1392
Mox20I	TGGCCA	1 cut(s)	1495
MroNI	GCCGGC	1 cut(s)	980
MscI	TGGCCA	1 cut(s)	1495
MseI	TTAA	4 cut(s)	140, 210, 816, 1299
Msp20I	TGGCCA	1 cut(s)	1495
MspI	CCGG	4 cut(s)	232, 981, 1278, 1581
MspR9I	CCNGG	9 cut(s)	173, 232, 912, 1211, 1233, 1278, 1279, 1413, 1497
Mva1269I	GAATGC	2 cut(s)	104, 1112
MvaI	CCWGG	6 cut(s)	173, 912, 1211, 1233, 1413, 1497
MwoI	GCNNNNNNNGC	7 cut(s)	492, 755, 998, 1056, 1211, 1448, 1523
NaeI	GCCGGC	1 cut(s)	982
NciI	CCSGG	3 cut(s)	232, 1278, 1279
NcoI	CCATGG	1 cut(s)	1331
NdeII	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
NgoMIV	GCCGGC	1 cut(s)	980
NlaIII	CATG	7 cut(s)	188, 423, 910, 1335, 1363, 1508, 1569
NlaIV	GGNNCC	6 cut(s)	26, 46, 170, 177, 985, 1417
NmuCI	GTSAC	1 cut(s)	1585
NspI	RCATGY	1 cut(s)	188
PaeR7I	CTCGAG	1 cut(s)	954
PciI	ACATGT	1 cut(s)	184
PciSI	GCTCTTC	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	3 cut(s)	1125, 1152, 1350
PctI	GAATGC	2 cut(s)	104, 1112
PdiI	GCCGGC	1 cut(s)	982
PfeI	GAWTC	5 cut(s)	287, 305, 502, 881, 945
PflFI	GACNNNGTC	2 cut(s)	343, 739
PinAI	ACCGGT	1 cut(s)	1580
PscI	ACATGT	1 cut(s)	184
PsiI	TTATAA	1 cut(s)	804
Psp6I	CCWGG	6 cut(s)	171, 910, 1209, 1231, 1411, 1495
PspFI	CCCAGC	1 cut(s)	119
PspGI	CCWGG	6 cut(s)	171, 910, 1209, 1231, 1411, 1495
PspN4I	GGNNCC	6 cut(s)	26, 46, 170, 177, 985, 1417
PspPI	GGNCC	3 cut(s)	45, 983, 1224
PstNI	CAGNNNCTG	2 cut(s)	56, 764
PsuI	RGATCY	2 cut(s)	175, 1460
PsyI	GACNNNGTC	2 cut(s)	343, 739
RsaI	GTAC	4 cut(s)	36, 77, 1013, 1179
RsaNI	GTAC	4 cut(s)	35, 76, 1012, 1178
SapI	GCTCTTC	1 cut(s)	324
SaqAI	TTAA	4 cut(s)	140, 210, 816, 1299
Sau3AI	GATC	5 cut(s)	175, 719, 1024, 1269, 1460
Sau96I	GGNCC	3 cut(s)	45, 983, 1224
ScrFI	CCNGG	9 cut(s)	173, 232, 912, 1211, 1233, 1278, 1279, 1413, 1497
SduI	GDGCHC	2 cut(s)	171, 1278
SfaNI	GCATC	2 cut(s)	1059, 1134
SfcI	CTRYAG	3 cut(s)	1099, 1264, 1309
Sfr274I	CTCGAG	1 cut(s)	954
SinI	GGWCC	1 cut(s)	1224
SlaI	CTCGAG	1 cut(s)	954
SmaI	CCCGGG	1 cut(s)	1279
SmlI	CTYRAG	2 cut(s)	380, 954
SmoI	CTYRAG	2 cut(s)	380, 954
Sse9I	AATT	5 cut(s)	257, 269, 589, 1094, 1509
SsiI	CCGC	8 cut(s)	432, 468, 1090, 1149, 1152, 1183, 1218, 1442
SspI	AATATT	1 cut(s)	860
SspMI	CTAG	4 cut(s)	492, 885, 1004, 1347
StyD4I	CCNGG	9 cut(s)	171, 230, 910, 1209, 1231, 1276, 1277, 1411, 1495
StyI	CCWWGG	2 cut(s)	66, 1331
TaaI	ACNGT	7 cut(s)	326, 578, 688, 739, 931, 1265, 1540
TaiI	ACGT	4 cut(s)	455, 702, 842, 1383
TaqI	TCGA	5 cut(s)	513, 584, 955, 1128, 1577
TasI	AATT	5 cut(s)	257, 269, 589, 1094, 1509
TatI	WGTACW	1 cut(s)	1011
TauI	GCSGC	4 cut(s)	434, 470, 1151, 1154
TfiI	GAWTC	5 cut(s)	287, 305, 502, 881, 945
Tru1I	TTAA	4 cut(s)	140, 210, 816, 1299
Tru9I	TTAA	4 cut(s)	140, 210, 816, 1299
TscAI	CASTG	2 cut(s)	57, 1496
TseFI	GTSAC	1 cut(s)	1585
TseI	GCWGC	6 cut(s)	62, 102, 312, 758, 1122, 1570
Tsp45I	GTSAC	1 cut(s)	1585
TspDTI	ATGAA	5 cut(s)	240, 937, 1123, 1493, 1554
TspGWI	ACGGA	3 cut(s)	272, 1021, 1243
TspMI	CCCGGG	1 cut(s)	1277
TspRI	CASTG	2 cut(s)	57, 1496
Tth111I	GACNNNGTC	2 cut(s)	343, 739
VpaK11BI	GGWCC	1 cut(s)	1224
XapI	RAATTY	1 cut(s)	257
XceI	RCATGY	1 cut(s)	188
XhoI	CTCGAG	1 cut(s)	954
XmaI	CCCGGG	1 cut(s)	1277
XmiI	GTMKAC	1 cut(s)	1535
XspI	CTAG	4 cut(s)	492, 885, 1004, 1347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.