MD02G1104200.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009155
Physical Location & Seq
Reverse (-)
8366621 .. 8368143
1523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1104200.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGTAGTTGTGACTATATATGAATATGAGCAATTGATCAAGTTAATTACTGCCTGTGTGGCTCAAATTCATATAGTCGTGAAACTTGCAAAGACTACGCTGCTGATCATCAAGCAGAATATTTCTCAGATCGGCAAACGTATAGCCGATGCCTCTGAGTCGGTAAGTAACCTACTAGCTCTTGTCAACCGTATATCAAATCACCATATTAATGCAGAAACTGAGAAACAAGGAGTGTTTCCCAAATGGATATCACCCAGGGACCGGAAACTACTTCAGGCAACCACCATAAAAGCCAATGCCGTTGTCGCTAAAGATGGATCCGGCGACTATAAAACTGTATCAGAAGCCATTGGCGCAGCTTCCGGGGGTCGGTTTGTGATTCATGTAAAGGCGGGAGTTTATGAGGAGAAGATTCGCACTAACAAAGATGGTATTACGTTGATAGGAGAGGGAAAAGATTCCACTATTATTACTGGGGATTTTAGCGTTGCTAATGGTGCCACCATGCCTGGCTCAGCTACATTCACCATAACAGGAGATGGGTTCATTGCTAGGGATATAGGGTTCCAAAACACAGCAGGCCCTCAAGGAGAACAAGCCTTGGCTCTCTACATCGCTTCTGATCACTCAGTTCTCTACAGATGCAGCATTGTTGGGTACCAAGACACTCTCTATGCACTCGCCTTCCGTCAATTCTACATAGAATGTGACATCTACGGCACCATAGACTTCATCTTTGGCAACGCCGCTGCCGTTTTACAGAGTTGCAACCTGGTGCTTCGCAAGAACAAGGGCTACAATGCAATCCTGGCAAACGGGAGGACCGACCCTGGACAAAAAACCGGATTCTCGGTCCAAAACTGTAGGATCGTACCCAGCTTGGACTTCTCTCTGGTTAAGCACTCCTATAGTGTTGTGCTAGGATAG

Protein Analysis

310

Amino Acids

33.4

Weight (kDa)

8.84

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 100 - 309 1.4e-76 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 660
AccB1I GGYRCC 3 cut(s) 500, 660, 722
AciI CCGC 2 cut(s) 395, 750
AclWI GGATC 3 cut(s) 315, 328, 878
AcsI RAATTY 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 2 cut(s) 662, 876
AfiI CCNNNNNNNGG 2 cut(s) 264, 372
AjnI CCWGG 5 cut(s) 257, 511, 774, 810, 832
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 4 cut(s) 179, 362, 521, 882
AluI AGCT 4 cut(s) 179, 362, 521, 882
AlwI GGATC 3 cut(s) 315, 328, 878
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 4 cut(s) 100, 359, 648, 752
ApoI RAATTY 1 cut(s) 66
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AseI ATTAAT 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 460
Asp718I GGTACC 1 cut(s) 660
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 4 cut(s) 262, 584, 825, 856
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 3 cut(s) 194, 246, 520
AvaII GGWCC 3 cut(s) 262, 825, 856
BamHI GGATCC 1 cut(s) 320
BanI GGYRCC 3 cut(s) 500, 660, 722
BbvI GCAGC 4 cut(s) 87, 371, 660, 739
BccI CCATC 3 cut(s) 311, 425, 536
BceAI ACGGC 3 cut(s) 287, 736, 740
BciT130I CCWGG 5 cut(s) 259, 513, 776, 812, 834
BclI TGATCA 3 cut(s) 36, 105, 625
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 3 cut(s) 176, 555, 923
BfmI CTRYAG 3 cut(s) 640, 865, 910
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 5 cut(s) 101, 360, 649, 750, 753
BlpI GCTNAGC 1 cut(s) 517
BlsI GCNGC 5 cut(s) 102, 361, 650, 751, 754
Bme1390I CCNGG 6 cut(s) 259, 367, 513, 776, 812, 834
Bme18I GGWCC 3 cut(s) 262, 825, 856
BmgT120I GGNCC 4 cut(s) 262, 584, 825, 856
BmiI GGNNCC 6 cut(s) 263, 322, 502, 569, 662, 724
BmrFI CCNGG 6 cut(s) 259, 367, 513, 776, 812, 834
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 139, 635
BmuI ACTGGG 1 cut(s) 486
Bpu1102I GCTNAGC 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 367
BsaJI CCNNGG 5 cut(s) 257, 258, 366, 603, 832
BsaWI WCCGGW 2 cut(s) 264, 845
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 372
Bse1I ACTGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 5 cut(s) 259, 513, 776, 812, 834
BseDI CCNNGG 5 cut(s) 257, 258, 366, 603, 832
BseLI CCNNNNNNNGG 2 cut(s) 264, 372
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 5 cut(s) 140, 147, 213, 531, 645
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 4 cut(s) 87, 371, 660, 739
BseYI CCCAGC 1 cut(s) 878
BshFI GGCC 1 cut(s) 585
BshNI GGYRCC 3 cut(s) 500, 660, 722
BsiSI CCGG 4 cut(s) 265, 324, 366, 846
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 2 cut(s) 264, 372
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 1 cut(s) 585
Bsp143I GATC 6 cut(s) 36, 105, 129, 320, 625, 870
Bsp1720I GCTNAGC 1 cut(s) 517
BspACI CCGC 2 cut(s) 395, 750
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 5 cut(s) 139, 148, 214, 530, 644
BspLI GGNNCC 6 cut(s) 263, 322, 502, 569, 662, 724
BspPI GGATC 3 cut(s) 315, 328, 878
BspT107I GGYRCC 3 cut(s) 500, 660, 722
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 5 cut(s) 257, 258, 366, 603, 832
BssMI GATC 6 cut(s) 36, 105, 129, 320, 625, 870
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 5 cut(s) 259, 513, 776, 812, 834
Bst4CI ACNGT 3 cut(s) 191, 340, 866
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 5 cut(s) 126, 156, 222, 517, 631
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 6 cut(s) 39, 108, 132, 323, 628, 873
BstMBI GATC 6 cut(s) 36, 105, 129, 320, 625, 870
BstMWI GCNNNNNNNGC 5 cut(s) 59, 308, 356, 500, 812
BstNI CCWGG 5 cut(s) 259, 513, 776, 812, 834
BstSCI CCNGG 6 cut(s) 257, 365, 511, 774, 810, 832
BstSFI CTRYAG 3 cut(s) 640, 865, 910
BstV1I GCAGC 4 cut(s) 87, 371, 660, 739
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 601
Cac8I GCNNGC 1 cut(s) 583
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 4 cut(s) 262, 584, 825, 856
CsiI ACCWGGT 1 cut(s) 774
Csp6I GTAC 2 cut(s) 661, 875
CviAII CATG 2 cut(s) 386, 508
CviQI GTAC 2 cut(s) 661, 875
DdeI CTNAG 5 cut(s) 126, 156, 222, 517, 631
DpnI GATC 6 cut(s) 38, 107, 131, 322, 627, 872
DpnII GATC 6 cut(s) 36, 105, 129, 320, 625, 870
Eco130I CCWWGG 1 cut(s) 603
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 3 cut(s) 262, 825, 856
Eco57I CTGAAG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 5 cut(s) 257, 511, 774, 810, 832
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 2 cut(s) 389, 511
FaqI GGGAC 1 cut(s) 275
FatI CATG 2 cut(s) 385, 507
FauI CCCGC 1 cut(s) 388
FbaI TGATCA 3 cut(s) 36, 105, 625
Fnu4HI GCNGC 5 cut(s) 101, 360, 649, 750, 753
Fsp4HI GCNGC 5 cut(s) 101, 360, 649, 750, 753
FspBI CTAG 3 cut(s) 176, 555, 923
GlaI GCGC 1 cut(s) 358
GluI GCNGC 5 cut(s) 101, 360, 649, 750, 753
GsaI CCCAGC 1 cut(s) 882
HaeIII GGCC 1 cut(s) 585
HapII CCGG 4 cut(s) 265, 324, 366, 846
HhaI GCGC 1 cut(s) 359
Hin1II CATG 2 cut(s) 389, 511
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 158, 382, 415, 461, 849
HpaII CCGG 4 cut(s) 265, 324, 366, 846
HphI GGTGA 3 cut(s) 194, 246, 520
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 129, 157, 346, 625
Hpy188III TCNNGA 1 cut(s) 79
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 697
HpyCH4III ACNGT 3 cut(s) 191, 340, 866
HpyCH4IV ACGT 2 cut(s) 139, 440
HpyCH4V TGCA 6 cut(s) 89, 215, 648, 680, 771, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 308, 356, 500, 812
HpyF3I CTNAG 5 cut(s) 126, 156, 222, 517, 631
HpySE526I ACGT 2 cut(s) 139, 440
Hsp92II CATG 2 cut(s) 389, 511
HspAI GCGC 1 cut(s) 357
KpnI GGTACC 1 cut(s) 664
Ksp22I TGATCA 3 cut(s) 36, 105, 625
Kzo9I GATC 6 cut(s) 36, 105, 129, 320, 625, 870
Lsp1109I GCAGC 4 cut(s) 87, 371, 660, 739
LweI GCATC 2 cut(s) 139, 635
MabI ACCWGGT 1 cut(s) 774
MaeI CTAG 3 cut(s) 176, 555, 923
MaeII ACGT 2 cut(s) 139, 440
MaeIII GTNAC 3 cut(s) 10, 167, 710
MalI GATC 6 cut(s) 38, 107, 131, 322, 627, 872
MboI GATC 6 cut(s) 36, 105, 129, 320, 625, 870
MboII GAAGA 1 cut(s) 424
MfeI CAATTG 1 cut(s) 32
MflI RGATCY 1 cut(s) 320
MluCI AATT 4 cut(s) 32, 45, 66, 695
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 5 cut(s) 163, 400, 445, 597, 816
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 3 cut(s) 44, 210, 900
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 4 cut(s) 265, 324, 366, 846
MspR9I CCNGG 6 cut(s) 259, 367, 513, 776, 812, 834
MunI CAATTG 1 cut(s) 32
MvaI CCWGG 5 cut(s) 259, 513, 776, 812, 834
MwoI GCNNNNNNNGC 5 cut(s) 59, 308, 356, 500, 812
NciI CCSGG 1 cut(s) 367
NdeII GATC 6 cut(s) 36, 105, 129, 320, 625, 870
NlaIII CATG 2 cut(s) 389, 511
NlaIV GGNNCC 6 cut(s) 263, 322, 502, 569, 662, 724
NmuCI GTSAC 2 cut(s) 10, 710
PasI CCCWGGG 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 753, 825
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 4 cut(s) 382, 415, 461, 849
PkrI GCNGC 5 cut(s) 102, 361, 650, 751, 754
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PshBI ATTAAT 1 cut(s) 210
Psp6I CCWGG 5 cut(s) 257, 511, 774, 810, 832
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 5 cut(s) 257, 511, 774, 810, 832
PspN4I GGNNCC 6 cut(s) 263, 322, 502, 569, 662, 724
PspPI GGNCC 4 cut(s) 262, 584, 825, 856
PsrI GAACNNNNNNTAC 2 cut(s) 782, 814
PstNI CAGNNNCTG 1 cut(s) 221
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 2 cut(s) 662, 876
RsaNI GTAC 2 cut(s) 661, 875
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 3 cut(s) 44, 210, 900
SatI GCNGC 5 cut(s) 101, 360, 649, 750, 753
Sau3AI GATC 6 cut(s) 36, 105, 129, 320, 625, 870
Sau96I GGNCC 4 cut(s) 262, 584, 825, 856
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 6 cut(s) 259, 367, 513, 776, 812, 834
SetI ASST 8 cut(s) 142, 174, 181, 364, 443, 523, 777, 884
SexAI ACCWGGT 1 cut(s) 774
SfaNI GCATC 2 cut(s) 139, 635
SfcI CTRYAG 3 cut(s) 640, 865, 910
SinI GGWCC 3 cut(s) 262, 825, 856
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 4 cut(s) 32, 45, 66, 695
SsiI CCGC 2 cut(s) 395, 750
SspI AATATT 1 cut(s) 121
SspMI CTAG 3 cut(s) 176, 555, 923
StyD4I CCNGG 6 cut(s) 257, 365, 511, 774, 810, 832
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 3 cut(s) 191, 340, 866
TaiI ACGT 2 cut(s) 142, 443
TaqII GACCGA 2 cut(s) 842, 844
TasI AATT 4 cut(s) 32, 45, 66, 695
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 4 cut(s) 382, 415, 461, 849
Tru1I TTAA 3 cut(s) 44, 210, 900
Tru9I TTAA 3 cut(s) 44, 210, 900
TseFI GTSAC 2 cut(s) 10, 710
TseI GCWGC 4 cut(s) 100, 359, 648, 752
Tsp45I GTSAC 2 cut(s) 10, 710
TspDTI ATGAA 5 cut(s) 36, 59, 374, 538, 724
TspGWI ACGGA 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 262, 825, 856
VspI ATTAAT 1 cut(s) 210
XapI RAATTY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 3 cut(s) 176, 555, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.