Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

8770590 .. 8828821

Gene Structure 58232 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG107400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGTTTCAGTTTGTGGTTTTAGGGTTTCTTTGGGTACTGGGAGGGGCCTCAGTGTCTGGGGCTGCCTTGGAGAAGTACCAAGTGCATGTTAAAAAAGAATGCAGCTTTACTAGATATCCCAGCATTTGTGTTCAAACCTTGATGGGGTTGGGTGGTTCTGGGCAACAGGATCCTCAACAGGTTGATATAATGTTGGCTCTTGTCAACAAGACCATTTATGAGACCCGGTTGGCCACCTCCGAGTTTTCCCAATTCAGCTCCAAAGTATCTGAAGTCCGTGAAGCTCAACCCGCTCACCATTCTGTCAAAGACTACTGTGAAGAGTTAATGACCATGTCCTTGAAGCGCTTAGACCAATCCCTGATAGCTCTCAAGAAGTCTCCCTTGAAAAAAAAGCAAGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCAAGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGGTCGACCTTTCCAAGCGCATAGATTATGCCTCTCAGTTGGGAAGTAATCTATTAGCTCTCGTCAACCGTGTTACAAGTATCGGTACGCCGAAGAACACAATTCGCGATAAACAAGCAGAGGTTTTTCCCAAATGGGTTTCGCCGAAGAACCGGAAACTACTTCAGGCAACCACAGTCAAAGCGAACGCAGTGGTTGCACAAGATGGATCAGGGAACTATAAGACTGTGTCGGAGGCTATTCAGGCAGCTTCAGGGGGTCGGTTTGTGATTTATGTGAAGGCGGGAGTTTATAAGGAAAAGATTAAGACTAACAAAGATGGTATTACGTTGATTGGAGATGGAAAGTATTCGACTATTATTACTGGGGATTCTAGCGTCGCTAAAGGTGCTTCGATGCCTGGCTCAGCTACATTCACCGTAACAGGAGATGGGTTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAAGTGTAGCATTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTCTACAGAGAATGTGACATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTTATCTTGCGCAGGCACAAGGGCTATGTTGCTATCCTGGCAAGCGGGAGGTCCGACCCGGGGCAAAAGACGGGCTTCTCTGTCCAGAACTGTAGGATTCTGCCCGGGTCAGACTTATCTCCTGTTAAACATTCCTACAGTTCGTATCTTGGGAGGCCATGGAAGGCCTACGCTAGAGCCGTGGTGATGCAGTCCAACATAGACGACGTTATCGCTCCCAGAGGCTGGGTCGAGTGGCCTGAGGCTGGAGGTTCCAGCCTCAAGTCACTCTACTTTGCGGAGTTCGCAAACATGGGGCCTGGGGCTGGAGTGGCCCAGAGGGTGCAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAGTACACTGTAGGTAACTTTATTGGTGGAACTTCATGGCTTCCTTCGACCGGCGTGACTTTTGTTTCCGGCCTCCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

535

Amino Acids

58.06

Weight (kDa)

9.06

Isoelectric Point (pI)

35.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	30 - 187	2.3e-22	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	229 - 519	2.7e-114	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	792
Acc16I	TGCGCA	1 cut(s)	1172
AccB7I	CCANNNNNTGG	1 cut(s)	1386
AccBSI	CCGCTC	1 cut(s)	293
AccI	GTMKAC	1 cut(s)	504
AccII	CGCG	1 cut(s)	606
AciI	CCGC	9 cut(s)	291, 423, 459, 782, 1078, 1137, 1140, 1206, 1439
AclWI	GGATC	3 cut(s)	164, 177, 715
AcoI	YGGCCR	2 cut(s)	231, 1490
AcuI	CTGAAG	3 cut(s)	291, 647, 735
AfaI	GTAC	5 cut(s)	36, 77, 586, 1001, 1532
AfeI	AGCGCT	1 cut(s)	347
AfiI	CCNNNNNNNGG	6 cut(s)	144, 1221, 1386, 1406, 1466, 1577
AgsI	TTSAA	3 cut(s)	134, 343, 388
AjnI	CCWGG	4 cut(s)	898, 1197, 1459, 1492
Alw26I	GTCTC	2 cut(s)	215, 384
AlwI	GGATC	3 cut(s)	164, 177, 715
AlwNI	CAGNNNCTG	3 cut(s)	56, 974, 1386
Ama87I	CYCGRG	3 cut(s)	942, 1219, 1265
Aor51HI	AGCGCT	1 cut(s)	347
ApeKI	GCWGC	4 cut(s)	62, 102, 746, 1110
ArsI	GACNNNNNNTTYG	2 cut(s)	1109, 1141
Asp700I	GAANNNNTTC	1 cut(s)	847
AspLEI	GCGC	3 cut(s)	348, 519, 1173
AspS9I	GGNCC	5 cut(s)	45, 971, 1212, 1457, 1474
AsuC2I	CCSGG	5 cut(s)	226, 1220, 1221, 1266, 1267
AsuHPI	GGTGA	3 cut(s)	287, 907, 1357
AvaI	CYCGRG	3 cut(s)	942, 1219, 1265
AvaII	GGWCC	1 cut(s)	1212
AxyI	CCTNAGG	1 cut(s)	1401
BalI	TGGCCA	2 cut(s)	233, 1492
BamHI	GGATCC	1 cut(s)	169
BbsI	GAAGAC	1 cut(s)	1138
BbvCI	CCTCAGC	1 cut(s)	473
BbvI	GCAGC	4 cut(s)	49, 114, 758, 1122
BccI	CCATC	6 cut(s)	136, 698, 812, 833, 923, 971
BceAI	ACGGC	3 cut(s)	484, 1127, 1325
BciT130I	CCWGG	4 cut(s)	900, 1199, 1461, 1494
BclI	TGATCA	1 cut(s)	1012
BcnI	CCSGG	5 cut(s)	226, 1220, 1221, 1266, 1267
BcoDI	GTCTC	2 cut(s)	215, 384
BfaI	CTAG	4 cut(s)	111, 483, 873, 1335
BfmI	CTRYAG	4 cut(s)	1087, 1252, 1297, 1536
BfoI	RGCGCY	1 cut(s)	349
BisI	GCNGC	8 cut(s)	63, 103, 423, 459, 747, 1111, 1137, 1140
BlpI	GCTNAGC	1 cut(s)	904
BlsI	GCNGC	8 cut(s)	64, 104, 424, 460, 748, 1112, 1138, 1141
Bme1390I	CCNGG	9 cut(s)	226, 900, 1199, 1220, 1221, 1266, 1267, 1461, 1494
Bme18I	GGWCC	1 cut(s)	1212
BmeT110I	CYCGRG	3 cut(s)	942, 1219, 1265
BmgT120I	GGNCC	5 cut(s)	45, 971, 1212, 1457, 1474
BmiI	GGNNCC	4 cut(s)	46, 171, 1414, 1458
BmrFI	CCNGG	9 cut(s)	226, 900, 1199, 1220, 1221, 1266, 1267, 1461, 1494
BmrI	ACTGGG	2 cut(s)	47, 873
BmsI	GCATC	2 cut(s)	885, 1338
BmuI	ACTGGG	2 cut(s)	47, 873
BpiI	GAAGAC	1 cut(s)	1138
BpmI	CTGGAG	2 cut(s)	1428, 1488
Bpu10I	CCTNAGC	2 cut(s)	473, 991
Bpu1102I	GCTNAGC	1 cut(s)	904
BpuEI	CTTGAG	2 cut(s)	356, 1406
BpuMI	CCSGG	5 cut(s)	226, 1220, 1221, 1266, 1267
BsaI	GGTCTC	1 cut(s)	215
BsaJI	CCNNGG	9 cut(s)	66, 466, 1219, 1220, 1265, 1319, 1341, 1460, 1493
BsaWI	WCCGGW	1 cut(s)	651
BsaXI	ACNNNNNCTCC	2 cut(s)	1059, 1089
Bsc4I	CCNNNNNNNGG	6 cut(s)	144, 1221, 1386, 1406, 1466, 1577
Bse118I	RCCGGY	1 cut(s)	1577
Bse1I	ACTGG	2 cut(s)	42, 868
Bse21I	CCTNAGG	1 cut(s)	1401
BseBI	CCWGG	4 cut(s)	900, 1199, 1461, 1494
BseDI	CCNNGG	9 cut(s)	66, 466, 1219, 1220, 1265, 1319, 1341, 1460, 1493
BseLI	CCNNNNNNNGG	6 cut(s)	144, 1221, 1386, 1406, 1466, 1577
BseMII	CTCAG	7 cut(s)	63, 487, 511, 548, 918, 1032, 1392
BseNI	ACTGG	2 cut(s)	42, 868
BseXI	GCAGC	4 cut(s)	49, 114, 758, 1122
BseYI	CCCAGC	2 cut(s)	119, 1386
BsgI	GTGCAG	1 cut(s)	1505
Bsh1236I	CGCG	1 cut(s)	606
Bsh1285I	CGRYCG	1 cut(s)	1578
BsiEI	CGRYCG	1 cut(s)	1578
BsiHKCI	CYCGRG	3 cut(s)	942, 1219, 1265
BsiSI	CCGG	6 cut(s)	226, 652, 1220, 1266, 1578, 1596
BslI	CCNNNNNNNGG	6 cut(s)	144, 1221, 1386, 1406, 1466, 1577
BsmAI	GTCTC	2 cut(s)	215, 384
BsmI	GAATGC	1 cut(s)	104
Bso31I	GGTCTC	1 cut(s)	215
BsoBI	CYCGRG	3 cut(s)	942, 1219, 1265
Bsp1407I	TGTACA	1 cut(s)	999
Bsp143I	GATC	3 cut(s)	169, 707, 1012
Bsp1720I	GCTNAGC	1 cut(s)	904
Bsp19I	CCATGG	1 cut(s)	1319
Bsp68I	TCGCGA	1 cut(s)	606
BspACI	CCGC	9 cut(s)	291, 423, 459, 782, 1078, 1137, 1140, 1206, 1439
BspCNI	CTCAG	7 cut(s)	62, 486, 510, 547, 917, 1031, 1393
BspFNI	CGCG	1 cut(s)	606
BspLI	GGNNCC	4 cut(s)	46, 171, 1414, 1458
BspPI	GGATC	3 cut(s)	164, 177, 715
BspTNI	GGTCTC	1 cut(s)	215
BsrBI	CCGCTC	1 cut(s)	293
BsrFI	RCCGGY	1 cut(s)	1577
BsrGI	TGTACA	1 cut(s)	999
BsrI	ACTGG	2 cut(s)	42, 868
BssAI	RCCGGY	1 cut(s)	1577
BssECI	CCNNGG	9 cut(s)	66, 466, 1219, 1220, 1265, 1319, 1341, 1460, 1493
BssMI	GATC	3 cut(s)	169, 707, 1012
BssT1I	CCWWGG	2 cut(s)	66, 1319
Bst2UI	CCWGG	4 cut(s)	900, 1199, 1461, 1494
Bst4CI	ACNGT	8 cut(s)	317, 569, 676, 727, 919, 1253, 1301, 1537
Bst6I	CTCTTC	2 cut(s)	315, 1012
BstAPI	GCANNNNNTGC	1 cut(s)	695
BstAUI	TGTACA	1 cut(s)	999
BstC8I	GCNNGC	3 cut(s)	970, 1175, 1204
BstDSI	CCRYGG	3 cut(s)	466, 1319, 1341
BstFNI	CGCG	1 cut(s)	606
BstH2I	RGCGCY	1 cut(s)	349
BstHHI	GCGC	3 cut(s)	348, 519, 1173
BstKTI	GATC	3 cut(s)	172, 710, 1015
BstMAI	GTCTC	2 cut(s)	215, 384
BstMBI	GATC	3 cut(s)	169, 707, 1012
BstMCI	CGRYCG	1 cut(s)	1578
BstNI	CCWGG	4 cut(s)	900, 1199, 1461, 1494
BstNSI	RCATGY	1 cut(s)	89
BstSCI	CCNGG	9 cut(s)	224, 898, 1197, 1218, 1219, 1264, 1265, 1459, 1492
BstSFI	CTRYAG	4 cut(s)	1087, 1252, 1297, 1536
BstUI	CGCG	1 cut(s)	606
BstV1I	GCAGC	4 cut(s)	49, 114, 758, 1122
BstV2I	GAAGAC	1 cut(s)	1138
BstX2I	RGATCY	1 cut(s)	169
BstYI	RGATCY	1 cut(s)	169
Bsu36I	CCTNAGG	1 cut(s)	1401
BtgI	CCRYGG	3 cut(s)	466, 1319, 1341
BtsI	GCAGTG	2 cut(s)	696, 1493
BtsIMutI	CAGTG	4 cut(s)	57, 696, 1493, 1533
BtuMI	TCGCGA	1 cut(s)	606
Cac8I	GCNNGC	3 cut(s)	970, 1175, 1204
CaiI	CAGNNNCTG	3 cut(s)	56, 974, 1386
CfoI	GCGC	3 cut(s)	348, 519, 1173
Cfr10I	RCCGGY	1 cut(s)	1577
Cfr13I	GGNCC	5 cut(s)	45, 971, 1212, 1457, 1474
Cfr9I	CCCGGG	2 cut(s)	1219, 1265
CseI	GACGC	1 cut(s)	865
Csp6I	GTAC	5 cut(s)	35, 76, 585, 1000, 1531
CviAII	CATG	8 cut(s)	86, 334, 411, 1106, 1320, 1453, 1502, 1563
CviQI	GTAC	5 cut(s)	35, 76, 585, 1000, 1531
DpnI	GATC	3 cut(s)	171, 709, 1014
DpnII	GATC	3 cut(s)	169, 707, 1012
EaeI	YGGCCR	2 cut(s)	231, 1490
Eam1104I	CTCTTC	2 cut(s)	315, 1012
EarI	CTCTTC	2 cut(s)	315, 1012
EciI	GGCGGA	1 cut(s)	1067
Eco130I	CCWWGG	2 cut(s)	66, 1319
Eco147I	AGGCCT	1 cut(s)	1328
Eco31I	GGTCTC	1 cut(s)	215
Eco32I	GATATC	1 cut(s)	116
Eco47I	GGWCC	1 cut(s)	1212
Eco47III	AGCGCT	1 cut(s)	347
Eco57I	CTGAAG	3 cut(s)	291, 647, 735
Eco81I	CCTNAGG	1 cut(s)	1401
Eco88I	CYCGRG	3 cut(s)	942, 1219, 1265
EcoO109I	RGGNCCY	3 cut(s)	45, 971, 1457
EcoRII	CCWGG	4 cut(s)	898, 1197, 1459, 1492
EcoRV	GATATC	1 cut(s)	116
EcoT14I	CCWWGG	2 cut(s)	66, 1319
ErhI	CCWWGG	2 cut(s)	66, 1319
FaeI	CATG	8 cut(s)	89, 337, 414, 1109, 1323, 1456, 1505, 1566
FalI	AAGNNNNNCTT	4 cut(s)	368, 400, 1220, 1252
FatI	CATG	8 cut(s)	85, 333, 410, 1105, 1319, 1452, 1501, 1562
FauI	CCCGC	3 cut(s)	298, 775, 1199
FbaI	TGATCA	1 cut(s)	1012
FblI	GTMKAC	1 cut(s)	504
Fnu4HI	GCNGC	8 cut(s)	63, 103, 423, 459, 747, 1111, 1137, 1140
Fsp4HI	GCNGC	8 cut(s)	63, 103, 423, 459, 747, 1111, 1137, 1140
FspBI	CTAG	4 cut(s)	111, 483, 873, 1335
FspI	TGCGCA	1 cut(s)	1172
GlaI	GCGC	3 cut(s)	347, 518, 1172
GluI	GCNGC	8 cut(s)	63, 103, 423, 459, 747, 1111, 1137, 1140
GsaI	CCCAGC	2 cut(s)	123, 1390
GsuI	CTGGAG	2 cut(s)	1428, 1488
HaeII	RGCGCY	1 cut(s)	349
HapII	CCGG	6 cut(s)	226, 652, 1220, 1266, 1578, 1596
HgaI	GACGC	1 cut(s)	865
HhaI	GCGC	3 cut(s)	348, 519, 1173
Hin1II	CATG	8 cut(s)	89, 337, 414, 1109, 1323, 1456, 1505, 1566
Hin6I	GCGC	3 cut(s)	346, 517, 1171
HinP1I	GCGC	3 cut(s)	346, 517, 1171
HincII	GTYRAC	3 cut(s)	205, 505, 565
HindII	GTYRAC	3 cut(s)	205, 505, 565
HinfI	GANTC	3 cut(s)	493, 869, 1258
HpaII	CCGG	6 cut(s)	226, 652, 1220, 1266, 1578, 1596
HphI	GGTGA	3 cut(s)	287, 907, 1357
Hpy166II	GTNNAC	4 cut(s)	205, 505, 565, 1533
Hpy188I	TCNGA	8 cut(s)	241, 271, 490, 733, 1012, 1130, 1216, 1273
Hpy188III	TCNNGA	5 cut(s)	373, 605, 944, 1150, 1246
Hpy8I	GTNNAC	4 cut(s)	205, 505, 565, 1533
Hpy99I	CGWCG	2 cut(s)	881, 1370
HpyAV	CCTTC	3 cut(s)	772, 1318, 1581
HpyCH4III	ACNGT	8 cut(s)	317, 569, 676, 727, 919, 1253, 1301, 1537
HpyCH4IV	ACGT	3 cut(s)	443, 827, 1368
HpyCH4V	TGCA	7 cut(s)	85, 102, 447, 698, 1158, 1351, 1486
HpySE526I	ACGT	3 cut(s)	443, 827, 1368
Hsp92II	CATG	8 cut(s)	89, 337, 414, 1109, 1323, 1456, 1505, 1566
HspAI	GCGC	3 cut(s)	346, 517, 1171
Ksp22I	TGATCA	1 cut(s)	1012
Kzo9I	GATC	3 cut(s)	169, 707, 1012
LmnI	GCTCC	4 cut(s)	263, 491, 1381, 1511
Lsp1109I	GCAGC	4 cut(s)	49, 114, 758, 1122
LweI	GCATC	2 cut(s)	885, 1338
MaeI	CTAG	4 cut(s)	111, 483, 873, 1335
MaeII	ACGT	3 cut(s)	443, 827, 1368
MaeIII	GTNAC	6 cut(s)	571, 919, 1097, 1425, 1541, 1582
MalI	GATC	3 cut(s)	171, 709, 1014
MbiI	CCGCTC	1 cut(s)	293
MboI	GATC	3 cut(s)	169, 707, 1012
MboII	GAAGA	6 cut(s)	332, 604, 658, 999, 1117, 1138
MflI	RGATCY	1 cut(s)	169
MlsI	TGGCCA	2 cut(s)	233, 1492
MluCI	AATT	3 cut(s)	251, 600, 1082
MluNI	TGGCCA	2 cut(s)	233, 1492
MmeI	TCCRAC	3 cut(s)	711, 1239, 1380
Mox20I	TGGCCA	2 cut(s)	233, 1492
MroXI	GAANNNNTTC	1 cut(s)	847
MscI	TGGCCA	2 cut(s)	233, 1492
MseI	TTAA	4 cut(s)	90, 326, 804, 1287
Msp20I	TGGCCA	2 cut(s)	233, 1492
MspI	CCGG	6 cut(s)	226, 652, 1220, 1266, 1578, 1596
MspR9I	CCNGG	9 cut(s)	226, 900, 1199, 1220, 1221, 1266, 1267, 1461, 1494
Mva1269I	GAATGC	1 cut(s)	104
MvaI	CCWGG	4 cut(s)	900, 1199, 1461, 1494
MvnI	CGCG	1 cut(s)	606
NciI	CCSGG	5 cut(s)	226, 1220, 1221, 1266, 1267
NcoI	CCATGG	1 cut(s)	1319
NdeII	GATC	3 cut(s)	169, 707, 1012
NlaIII	CATG	8 cut(s)	89, 337, 414, 1109, 1323, 1456, 1505, 1566
NlaIV	GGNNCC	4 cut(s)	46, 171, 1414, 1458
NmuCI	GTSAC	3 cut(s)	1097, 1425, 1582
NruI	TCGCGA	1 cut(s)	606
NsbI	TGCGCA	1 cut(s)	1172
NspI	RCATGY	1 cut(s)	89
PaeR7I	CTCGAG	1 cut(s)	942
PceI	AGGCCT	1 cut(s)	1328
PcsI	WCGNNNNNNNCGW	2 cut(s)	1140, 1338
PctI	GAATGC	1 cut(s)	104
PdmI	GAANNNNTTC	1 cut(s)	847
PfeI	GAWTC	3 cut(s)	493, 869, 1258
PflFI	GACNNNGTC	2 cut(s)	334, 727
PflMI	CCANNNNNTGG	1 cut(s)	1386
PkrI	GCNGC	8 cut(s)	64, 104, 424, 460, 748, 1112, 1138, 1141
PsiI	TTATAA	1 cut(s)	792
Psp6I	CCWGG	4 cut(s)	898, 1197, 1459, 1492
PspFI	CCCAGC	2 cut(s)	119, 1386
PspGI	CCWGG	4 cut(s)	898, 1197, 1459, 1492
PspN4I	GGNNCC	4 cut(s)	46, 171, 1414, 1458
PspPI	GGNCC	5 cut(s)	45, 971, 1212, 1457, 1474
PstNI	CAGNNNCTG	3 cut(s)	56, 974, 1386
PsuI	RGATCY	1 cut(s)	169
PsyI	GACNNNGTC	2 cut(s)	334, 727
RruI	TCGCGA	1 cut(s)	606
RsaI	GTAC	5 cut(s)	36, 77, 586, 1001, 1532
RsaNI	GTAC	5 cut(s)	35, 76, 585, 1000, 1531
SalI	GTCGAC	1 cut(s)	503
SaqAI	TTAA	4 cut(s)	90, 326, 804, 1287
SatI	GCNGC	8 cut(s)	63, 103, 423, 459, 747, 1111, 1137, 1140
Sau3AI	GATC	3 cut(s)	169, 707, 1012
Sau96I	GGNCC	5 cut(s)	45, 971, 1212, 1457, 1474
ScrFI	CCNGG	9 cut(s)	226, 900, 1199, 1220, 1221, 1266, 1267, 1461, 1494
SfaNI	GCATC	2 cut(s)	885, 1338
SfcI	CTRYAG	4 cut(s)	1087, 1252, 1297, 1536
Sfr274I	CTCGAG	1 cut(s)	942
SinI	GGWCC	1 cut(s)	1212
SlaI	CTCGAG	1 cut(s)	942
SmaI	CCCGGG	2 cut(s)	1221, 1267
SmlI	CTYRAG	3 cut(s)	371, 942, 1421
SmoI	CTYRAG	3 cut(s)	371, 942, 1421
Sse9I	AATT	3 cut(s)	251, 600, 1082
SseBI	AGGCCT	1 cut(s)	1328
SsiI	CCGC	9 cut(s)	291, 423, 459, 782, 1078, 1137, 1140, 1206, 1439
SspMI	CTAG	4 cut(s)	111, 483, 873, 1335
StuI	AGGCCT	1 cut(s)	1328
StyD4I	CCNGG	9 cut(s)	224, 898, 1197, 1218, 1219, 1264, 1265, 1459, 1492
StyI	CCWWGG	2 cut(s)	66, 1319
TaaI	ACNGT	8 cut(s)	317, 569, 676, 727, 919, 1253, 1301, 1537
TaiI	ACGT	3 cut(s)	446, 830, 1371
TaqI	TCGA	7 cut(s)	504, 851, 893, 943, 1116, 1392, 1574
TasI	AATT	3 cut(s)	251, 600, 1082
TatI	WGTACW	2 cut(s)	999, 1530
TauI	GCSGC	4 cut(s)	425, 461, 1139, 1142
TfiI	GAWTC	3 cut(s)	493, 869, 1258
Tru1I	TTAA	4 cut(s)	90, 326, 804, 1287
Tru9I	TTAA	4 cut(s)	90, 326, 804, 1287
TscAI	CASTG	4 cut(s)	57, 696, 1493, 1540
TseFI	GTSAC	3 cut(s)	1097, 1425, 1582
TseI	GCWGC	4 cut(s)	62, 102, 746, 1110
Tsp45I	GTSAC	3 cut(s)	1097, 1425, 1582
TspDTI	ATGAA	5 cut(s)	925, 1094, 1111, 1490, 1551
TspGWI	ACGGA	2 cut(s)	266, 1592
TspMI	CCCGGG	2 cut(s)	1219, 1265
TspRI	CASTG	4 cut(s)	57, 696, 1493, 1540
Tth111I	GACNNNGTC	2 cut(s)	334, 727
Van91I	CCANNNNNTGG	1 cut(s)	1386
VpaK11BI	GGWCC	1 cut(s)	1212
XceI	RCATGY	1 cut(s)	89
XhoI	CTCGAG	1 cut(s)	942
XmaI	CCCGGG	2 cut(s)	1219, 1265
XmiI	GTMKAC	1 cut(s)	504
XmnI	GAANNNNTTC	1 cut(s)	847
XspI	CTAG	4 cut(s)	111, 483, 873, 1335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.