Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

9114102 .. 9116303

Gene Structure 2202 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ47128

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGACCATGTCCTTGAAGCGCCTAGACCAATGCCTAATAGCTCTCAAGAAATCTCCCTTGAAAAACAAGCACGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCATGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGGTCGACCTTTCCAAGCGCATAGATTATGCCTCTCAGTTGGGAAGTAATCTATTAGCCCTCGTCAACCGTGTTACAAGTATCGGTGCGCCGAAGAACACAATTCGCGATAAACAAGCAGAGGTTTTTCCCAAATGGGTTTCGCCGAAGAACCGGAAACTACTTCAGGCAACCACAGTCAAAGCGAACGCAGTGGTTGCACAAGATGGATCAGGGAACTATAAGACTGTGTCGGAGGCTATTCAGGCAGCTTCAGGGGGTCGGTTTGTGATTTATGTGAAGGCGGGAGTTTATAAGGAAAAGATTAAGACTAACAAAGATGGTATTACGTTGATTGGAGATGGAAAGTATTCAACTATTATTACTGGGGATTCTAGCGTCGCTAAAGGTGCTTCCCTGCCTGGCTCAGCTACATTCACTGTAACAGGAGATGGATTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAGGTGTAGCGTTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTCTACAGAGAATGTGACATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTCATCTTCCGCAGGCACAAGGGCTATGGTGTTATCCTGGCAAGCGGGAGGTCCGACCCGGGGCAAAAGACGGGCTTCTCCGTCCAGAACTGTAGGATTCTGCCCGGGTCAGACTTATCTCCTGTTAAACATTCCTACAGTTCGTATCTTGGGAGGCCATGGAAGGCCTACGCTAGAGCCGTGGTGATGCAGTCCAACATAGGTGACGTTATTGCTCCCGGTGGCTGGGTCCAGTGGCCTGGGGCTGAAGCTTCCAGGCTCAAGTCACTCTACTTTGCGGAGTTCGCAAACGTGGGACCTGGGGCTGGAGTGGCCCAGAGGGTGAAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAGTACACTGTAGGTAACTTTATTGGTGGAACTTCATGGCTTCCTTCGACCGGCGTGACTTTTGTTTCCGGCCTCCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

426

Amino Acids

45.87

Weight (kDa)

9.44

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	4 - 78	2.6e-08	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	120 - 410	8.1e-114	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	465
AccI	GTMKAC	1 cut(s)	177
AccII	CGCG	1 cut(s)	279
AciI	CCGC	9 cut(s)	96, 132, 455, 751, 810, 813, 844, 879, 1112
AclWI	GGATC	1 cut(s)	388
AcoI	YGGCCR	1 cut(s)	1163
AcuI	CTGAAG	3 cut(s)	320, 408, 1101
AfaI	GTAC	2 cut(s)	674, 1205
AfiI	CCNNNNNNNGG	4 cut(s)	894, 1059, 1139, 1250
AgsI	TTSAA	3 cut(s)	16, 61, 525
AjnI	CCWGG	6 cut(s)	571, 870, 1072, 1088, 1132, 1165
AluBI	AGCT	8 cut(s)	41, 159, 422, 581, 614, 668, 1085, 1187
AluI	AGCT	8 cut(s)	41, 159, 422, 581, 614, 668, 1085, 1187
AlwI	GGATC	1 cut(s)	388
AlwNI	CAGNNNCTG	1 cut(s)	647
Ama87I	CYCGRG	3 cut(s)	615, 892, 938
AoxI	GGCC	8 cut(s)	142, 643, 989, 999, 1070, 1146, 1163, 1270
ApeKI	GCWGC	2 cut(s)	419, 783
ArsI	GACNNNNNNTTYG	2 cut(s)	782, 814
Asp700I	GAANNNNTTC	1 cut(s)	520
AspLEI	GCGC	3 cut(s)	21, 192, 262
AspS9I	GGNCC	5 cut(s)	644, 885, 1063, 1130, 1147
AsuC2I	CCSGG	5 cut(s)	893, 894, 939, 940, 1053
AsuHPI	GGTGA	3 cut(s)	1030, 1049, 1168
AvaI	CYCGRG	3 cut(s)	615, 892, 938
AvaII	GGWCC	3 cut(s)	885, 1063, 1130
BalI	TGGCCA	1 cut(s)	1165
BbsI	GAAGAC	1 cut(s)	811
BbvCI	CCTCAGC	1 cut(s)	146
BbvI	GCAGC	2 cut(s)	431, 795
BccI	CCATC	5 cut(s)	371, 485, 506, 596, 644
BceAI	ACGGC	3 cut(s)	157, 800, 998
BciT130I	CCWGG	6 cut(s)	573, 872, 1074, 1090, 1134, 1167
BclI	TGATCA	1 cut(s)	685
BcnI	CCSGG	5 cut(s)	893, 894, 939, 940, 1053
BfaI	CTAG	4 cut(s)	23, 156, 546, 1008
BfmI	CTRYAG	5 cut(s)	700, 760, 925, 970, 1209
BfoI	RGCGCY	1 cut(s)	22
BisI	GCNGC	6 cut(s)	96, 132, 420, 784, 810, 813
BlpI	GCTNAGC	1 cut(s)	577
BlsI	GCNGC	6 cut(s)	97, 133, 421, 785, 811, 814
Bme18I	GGWCC	3 cut(s)	885, 1063, 1130
BmeT110I	CYCGRG	3 cut(s)	615, 892, 938
BmgT120I	GGNCC	5 cut(s)	644, 885, 1063, 1130, 1147
BmiI	GGNNCC	2 cut(s)	1064, 1131
BmrI	ACTGGG	1 cut(s)	546
BmsI	GCATC	1 cut(s)	1011
BmuI	ACTGGG	1 cut(s)	546
BpiI	GAAGAC	1 cut(s)	811
BpmI	CTGGAG	1 cut(s)	1161
Bpu10I	CCTNAGC	2 cut(s)	146, 664
Bpu1102I	GCTNAGC	1 cut(s)	577
BpuEI	CTTGAG	2 cut(s)	29, 1079
BpuMI	CCSGG	5 cut(s)	893, 894, 939, 940, 1053
BsaJI	CCNNGG	9 cut(s)	139, 892, 893, 938, 992, 1014, 1073, 1133, 1166
BsaWI	WCCGGW	1 cut(s)	324
BsaXI	ACNNNNNCTCC	2 cut(s)	732, 762
Bsc4I	CCNNNNNNNGG	4 cut(s)	894, 1059, 1139, 1250
Bse118I	RCCGGY	1 cut(s)	1250
Bse1I	ACTGG	2 cut(s)	541, 1066
BseBI	CCWGG	6 cut(s)	573, 872, 1074, 1090, 1134, 1167
BseDI	CCNNGG	9 cut(s)	139, 892, 893, 938, 992, 1014, 1073, 1133, 1166
BseLI	CCNNNNNNNGG	4 cut(s)	894, 1059, 1139, 1250
BseMII	CTCAG	5 cut(s)	160, 184, 221, 591, 705
BseNI	ACTGG	2 cut(s)	541, 1066
BseXI	GCAGC	2 cut(s)	431, 795
BseYI	CCCAGC	1 cut(s)	1059
Bsh1236I	CGCG	1 cut(s)	279
Bsh1285I	CGRYCG	1 cut(s)	1251
BshFI	GGCC	8 cut(s)	144, 645, 991, 1001, 1072, 1148, 1165, 1272
BsiEI	CGRYCG	1 cut(s)	1251
BsiHKCI	CYCGRG	3 cut(s)	615, 892, 938
BsiSI	CCGG	6 cut(s)	325, 893, 939, 1053, 1251, 1269
BslFI	GGGAC	1 cut(s)	1143
BslI	CCNNNNNNNGG	4 cut(s)	894, 1059, 1139, 1250
BsmFI	GGGAC	1 cut(s)	1143
BsnI	GGCC	8 cut(s)	144, 645, 991, 1001, 1072, 1148, 1165, 1272
BsoBI	CYCGRG	3 cut(s)	615, 892, 938
Bsp1407I	TGTACA	1 cut(s)	672
Bsp143I	GATC	2 cut(s)	380, 685
Bsp1720I	GCTNAGC	1 cut(s)	577
Bsp19I	CCATGG	1 cut(s)	992
Bsp68I	TCGCGA	1 cut(s)	279
BspACI	CCGC	9 cut(s)	96, 132, 455, 751, 810, 813, 844, 879, 1112
BspANI	GGCC	8 cut(s)	144, 645, 991, 1001, 1072, 1148, 1165, 1272
BspCNI	CTCAG	5 cut(s)	159, 183, 220, 590, 704
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	2 cut(s)	1064, 1131
BspPI	GGATC	1 cut(s)	388
BsrFI	RCCGGY	1 cut(s)	1250
BsrGI	TGTACA	1 cut(s)	672
BsrI	ACTGG	2 cut(s)	541, 1066
BssAI	RCCGGY	1 cut(s)	1250
BssECI	CCNNGG	9 cut(s)	139, 892, 893, 938, 992, 1014, 1073, 1133, 1166
BssMI	GATC	2 cut(s)	380, 685
BssT1I	CCWWGG	1 cut(s)	992
Bst2UI	CCWGG	6 cut(s)	573, 872, 1074, 1090, 1134, 1167
Bst4CI	ACNGT	7 cut(s)	242, 349, 400, 592, 926, 974, 1210
Bst6I	CTCTTC	1 cut(s)	685
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	672
BstC8I	GCNNGC	3 cut(s)	643, 848, 877
BstDEI	CTNAG	7 cut(s)	89, 146, 170, 207, 577, 664, 691
BstDSI	CCRYGG	3 cut(s)	139, 992, 1014
BstFNI	CGCG	1 cut(s)	279
BstH2I	RGCGCY	1 cut(s)	22
BstHHI	GCGC	3 cut(s)	21, 192, 262
BstKTI	GATC	2 cut(s)	383, 688
BstMBI	GATC	2 cut(s)	380, 685
BstMCI	CGRYCG	1 cut(s)	1251
BstMWI	GCNNNNNNNGC	8 cut(s)	156, 368, 416, 560, 717, 1118, 1145, 1193
BstNI	CCWGG	6 cut(s)	573, 872, 1074, 1090, 1134, 1167
BstSFI	CTRYAG	5 cut(s)	700, 760, 925, 970, 1209
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	2 cut(s)	431, 795
BstV2I	GAAGAC	1 cut(s)	811
BstXI	CCANNNNNNTGG	1 cut(s)	1073
BsuRI	GGCC	8 cut(s)	144, 645, 991, 1001, 1072, 1148, 1165, 1272
BtgI	CCRYGG	3 cut(s)	139, 992, 1014
BtsI	GCAGTG	1 cut(s)	369
BtsIMutI	CAGTG	4 cut(s)	369, 588, 1073, 1206
BtuMI	TCGCGA	1 cut(s)	279
Cac8I	GCNNGC	3 cut(s)	643, 848, 877
CaiI	CAGNNNCTG	1 cut(s)	647
CfoI	GCGC	3 cut(s)	21, 192, 262
Cfr10I	RCCGGY	1 cut(s)	1250
Cfr13I	GGNCC	5 cut(s)	644, 885, 1063, 1130, 1147
Cfr9I	CCCGGG	2 cut(s)	892, 938
CseI	GACGC	1 cut(s)	538
Csp6I	GTAC	2 cut(s)	673, 1204
CviAII	CATG	7 cut(s)	7, 84, 110, 779, 993, 1175, 1236
CviQI	GTAC	2 cut(s)	673, 1204
DdeI	CTNAG	7 cut(s)	89, 146, 170, 207, 577, 664, 691
DpnI	GATC	2 cut(s)	382, 687
DpnII	GATC	2 cut(s)	380, 685
EaeI	YGGCCR	1 cut(s)	1163
Eam1104I	CTCTTC	1 cut(s)	685
EarI	CTCTTC	1 cut(s)	685
EciI	GGCGGA	1 cut(s)	740
Eco130I	CCWWGG	1 cut(s)	992
Eco147I	AGGCCT	1 cut(s)	1001
Eco47I	GGWCC	3 cut(s)	885, 1063, 1130
Eco57I	CTGAAG	3 cut(s)	320, 408, 1101
Eco88I	CYCGRG	3 cut(s)	615, 892, 938
EcoO109I	RGGNCCY	2 cut(s)	644, 1130
EcoRII	CCWGG	6 cut(s)	571, 870, 1072, 1088, 1132, 1165
EcoT14I	CCWWGG	1 cut(s)	992
ErhI	CCWWGG	1 cut(s)	992
FaeI	CATG	7 cut(s)	10, 87, 113, 782, 996, 1178, 1239
FalI	AAGNNNNNCTT	2 cut(s)	893, 925
FaqI	GGGAC	1 cut(s)	1143
FatI	CATG	7 cut(s)	6, 83, 109, 778, 992, 1174, 1235
FauI	CCCGC	2 cut(s)	448, 872
FbaI	TGATCA	1 cut(s)	685
FblI	GTMKAC	1 cut(s)	177
Fnu4HI	GCNGC	6 cut(s)	96, 132, 420, 784, 810, 813
Fsp4HI	GCNGC	6 cut(s)	96, 132, 420, 784, 810, 813
FspBI	CTAG	4 cut(s)	23, 156, 546, 1008
GlaI	GCGC	3 cut(s)	20, 191, 261
GluI	GCNGC	6 cut(s)	96, 132, 420, 784, 810, 813
GsaI	CCCAGC	1 cut(s)	1063
GsuI	CTGGAG	1 cut(s)	1161
HaeII	RGCGCY	1 cut(s)	22
HaeIII	GGCC	8 cut(s)	144, 645, 991, 1001, 1072, 1148, 1165, 1272
HapII	CCGG	6 cut(s)	325, 893, 939, 1053, 1251, 1269
HgaI	GACGC	1 cut(s)	538
HhaI	GCGC	3 cut(s)	21, 192, 262
Hin1II	CATG	7 cut(s)	10, 87, 113, 782, 996, 1178, 1239
Hin6I	GCGC	3 cut(s)	19, 190, 260
HinP1I	GCGC	3 cut(s)	19, 190, 260
HincII	GTYRAC	2 cut(s)	178, 238
HindII	GTYRAC	2 cut(s)	178, 238
HindIII	AAGCTT	1 cut(s)	1083
HinfI	GANTC	4 cut(s)	166, 542, 606, 931
HpaII	CCGG	6 cut(s)	325, 893, 939, 1053, 1251, 1269
HphI	GGTGA	3 cut(s)	1030, 1049, 1168
Hpy166II	GTNNAC	3 cut(s)	178, 238, 1206
Hpy188I	TCNGA	6 cut(s)	163, 406, 685, 803, 889, 946
Hpy188III	TCNNGA	5 cut(s)	46, 278, 617, 823, 919
Hpy8I	GTNNAC	3 cut(s)	178, 238, 1206
Hpy99I	CGWCG	1 cut(s)	554
HpyAV	CCTTC	3 cut(s)	445, 991, 1254
HpyCH4III	ACNGT	7 cut(s)	242, 349, 400, 592, 926, 974, 1210
HpyCH4IV	ACGT	4 cut(s)	116, 500, 1041, 1125
HpyCH4V	TGCA	4 cut(s)	120, 371, 831, 1024
HpyF10VI	GCNNNNNNNGC	8 cut(s)	156, 368, 416, 560, 717, 1118, 1145, 1193
HpyF3I	CTNAG	7 cut(s)	89, 146, 170, 207, 577, 664, 691
HpySE526I	ACGT	4 cut(s)	116, 500, 1041, 1125
Hsp92II	CATG	7 cut(s)	10, 87, 113, 782, 996, 1178, 1239
HspAI	GCGC	3 cut(s)	19, 190, 260
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	2 cut(s)	380, 685
LmnI	GCTCC	3 cut(s)	164, 1054, 1184
Lsp1109I	GCAGC	2 cut(s)	431, 795
LweI	GCATC	1 cut(s)	1011
MaeI	CTAG	4 cut(s)	23, 156, 546, 1008
MaeII	ACGT	4 cut(s)	116, 500, 1041, 1125
MaeIII	GTNAC	7 cut(s)	244, 592, 770, 1037, 1098, 1214, 1255
MalI	GATC	2 cut(s)	382, 687
MboI	GATC	2 cut(s)	380, 685
MboII	GAAGA	6 cut(s)	277, 331, 672, 790, 811, 832
MlsI	TGGCCA	1 cut(s)	1165
MluCI	AATT	2 cut(s)	273, 755
MluNI	TGGCCA	1 cut(s)	1165
MmeI	TCCRAC	3 cut(s)	384, 912, 1053
Mox20I	TGGCCA	1 cut(s)	1165
MroXI	GAANNNNTTC	1 cut(s)	520
MscI	TGGCCA	1 cut(s)	1165
MseI	TTAA	2 cut(s)	477, 960
Msp20I	TGGCCA	1 cut(s)	1165
MspI	CCGG	6 cut(s)	325, 893, 939, 1053, 1251, 1269
MvaI	CCWGG	6 cut(s)	573, 872, 1074, 1090, 1134, 1167
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	8 cut(s)	156, 368, 416, 560, 717, 1118, 1145, 1193
NciI	CCSGG	5 cut(s)	893, 894, 939, 940, 1053
NcoI	CCATGG	1 cut(s)	992
NdeII	GATC	2 cut(s)	380, 685
NlaIII	CATG	7 cut(s)	10, 87, 113, 782, 996, 1178, 1239
NlaIV	GGNNCC	2 cut(s)	1064, 1131
NmuCI	GTSAC	4 cut(s)	770, 1037, 1098, 1255
NruI	TCGCGA	1 cut(s)	279
PaeR7I	CTCGAG	1 cut(s)	615
PceI	AGGCCT	1 cut(s)	1001
PcsI	WCGNNNNNNNCGW	2 cut(s)	813, 1011
PdmI	GAANNNNTTC	1 cut(s)	520
PfeI	GAWTC	4 cut(s)	166, 542, 606, 931
PflFI	GACNNNGTC	2 cut(s)	7, 400
PkrI	GCNGC	6 cut(s)	97, 133, 421, 785, 811, 814
PpuMI	RGGWCCY	1 cut(s)	1130
PsiI	TTATAA	1 cut(s)	465
Psp5II	RGGWCCY	1 cut(s)	1130
Psp6I	CCWGG	6 cut(s)	571, 870, 1072, 1088, 1132, 1165
PspFI	CCCAGC	1 cut(s)	1059
PspGI	CCWGG	6 cut(s)	571, 870, 1072, 1088, 1132, 1165
PspN4I	GGNNCC	2 cut(s)	1064, 1131
PspPI	GGNCC	5 cut(s)	644, 885, 1063, 1130, 1147
PspPPI	RGGWCCY	1 cut(s)	1130
PstNI	CAGNNNCTG	1 cut(s)	647
PsyI	GACNNNGTC	2 cut(s)	7, 400
RruI	TCGCGA	1 cut(s)	279
RsaI	GTAC	2 cut(s)	674, 1205
RsaNI	GTAC	2 cut(s)	673, 1204
SalI	GTCGAC	1 cut(s)	176
SaqAI	TTAA	2 cut(s)	477, 960
SatI	GCNGC	6 cut(s)	96, 132, 420, 784, 810, 813
Sau3AI	GATC	2 cut(s)	380, 685
Sau96I	GGNCC	5 cut(s)	644, 885, 1063, 1130, 1147
SfaNI	GCATC	1 cut(s)	1011
SfcI	CTRYAG	5 cut(s)	700, 760, 925, 970, 1209
Sfr274I	CTCGAG	1 cut(s)	615
SinI	GGWCC	3 cut(s)	885, 1063, 1130
SlaI	CTCGAG	1 cut(s)	615
SmaI	CCCGGG	2 cut(s)	894, 940
SmlI	CTYRAG	3 cut(s)	44, 615, 1094
SmoI	CTYRAG	3 cut(s)	44, 615, 1094
Sse9I	AATT	2 cut(s)	273, 755
SseBI	AGGCCT	1 cut(s)	1001
SsiI	CCGC	9 cut(s)	96, 132, 455, 751, 810, 813, 844, 879, 1112
SspMI	CTAG	4 cut(s)	23, 156, 546, 1008
StuI	AGGCCT	1 cut(s)	1001
StyI	CCWWGG	1 cut(s)	992
TaaI	ACNGT	7 cut(s)	242, 349, 400, 592, 926, 974, 1210
TaiI	ACGT	4 cut(s)	119, 503, 1044, 1128
TaqI	TCGA	4 cut(s)	177, 616, 789, 1247
TasI	AATT	2 cut(s)	273, 755
TatI	WGTACW	2 cut(s)	672, 1203
TauI	GCSGC	4 cut(s)	98, 134, 812, 815
TfiI	GAWTC	4 cut(s)	166, 542, 606, 931
Tru1I	TTAA	2 cut(s)	477, 960
Tru9I	TTAA	2 cut(s)	477, 960
TscAI	CASTG	4 cut(s)	369, 595, 1073, 1213
TseFI	GTSAC	4 cut(s)	770, 1037, 1098, 1255
TseI	GCWGC	2 cut(s)	419, 783
Tsp45I	GTSAC	4 cut(s)	770, 1037, 1098, 1255
TspDTI	ATGAA	6 cut(s)	126, 598, 767, 784, 1163, 1224
TspGWI	ACGGA	2 cut(s)	904, 1265
TspMI	CCCGGG	2 cut(s)	892, 938
TspRI	CASTG	4 cut(s)	369, 595, 1073, 1213
Tth111I	GACNNNGTC	2 cut(s)	7, 400
VpaK11BI	GGWCC	3 cut(s)	885, 1063, 1130
XhoI	CTCGAG	1 cut(s)	615
XmaI	CCCGGG	2 cut(s)	892, 938
XmiI	GTMKAC	1 cut(s)	177
XmnI	GAANNNNTTC	1 cut(s)	520
XspI	CTAG	4 cut(s)	23, 156, 546, 1008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.