Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

8410811 .. 8412682

Gene Structure 1872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016634

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGTCCTTGAAGCGCCTAGACCAATGCCTAATAGCTCTCAAGAAATCTCCCTTAAAAAACAAGCACGATATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCATGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGGTCGACCTTTCCAAGCGCATAGATTATGCCTCTCAGTTGGGAAGTAATCTATTAGCTCTCGTTAACCGTGTTACAAATATCGGTACGCCGAAGAGCACAATTCGCGATAAACAAGCAGAGGTTTTTCCCAAATGGGTTTCGCCGAAGAACCGGAAACTACTTCAGGCAACCATAGTCAAAGCGAACGCAGTGGTTGCACAAGATGGATCAGGGAACTATAAGACTGTGTCGGAAGCTATTCAGGCAGCTTCAGGGGGTCGGTTTGTGATTTATGTGAAGGCGGGAGTTTATAAGGAAAAGATTAAGACTAACAAAGATGGTATTACATTGATTGGAGATGGTAAGTATTCGACTATTATTTCTGGGGATTCTAGCGTCGCTAAAGGTGCTTCCCTGCCTGGCTCAGCTACATTCACCGTAACAGGAGATGGGTTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAGGTGTAGCATTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTCTACAGAGAATGTGACATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTCATCTTCCGCAGGCACAAGGGCTATGGTGTTATCCTGGCAAGCGGAAGGTCCGACCCGGGGCAAAAGACGGGCTTCTCCGTCCAGAACTGTAGGATTCTGCCCGGGTCAGACTTCTCTCCTGTTAAACATTCCTACAGTTCGTATCTTGGGAGGCCATGGAAGGCCTACGCTAGAGCCGTGGTGATGCAGTCCAACATAGACGAGGTTATCGCTCCCAGTGGCTGGGTCCAGTGGCCTGGGGCTGAAGCTTCCAGGCTCAAGTCACTCTACTTTGCGGAGTTCGCAAACGTGGGACCTGGGGCTGGAGTGGCCCAGAGGGTGAAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAGTACACTGTAGGTAACTTCATTGGTGGAACTTCATGGCTTCCTTCGACTGGTGTGACTTTTGTTTCTGGCCTCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

425

Amino Acids

45.79

Weight (kDa)

9.34

Isoelectric Point (pI)

32.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	2 - 76	4e-08	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	118 - 408	7.9e-114	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	459
AccB7I	CCANNNNNTGG	1 cut(s)	1053
AccI	GTMKAC	1 cut(s)	171
AccII	CGCG	1 cut(s)	273
AciI	CCGC	9 cut(s)	90, 126, 449, 745, 804, 807, 838, 873, 1106
AclWI	GGATC	1 cut(s)	382
AcoI	YGGCCR	1 cut(s)	1157
AcuI	CTGAAG	3 cut(s)	314, 402, 1095
AfaI	GTAC	3 cut(s)	253, 668, 1199
AfiI	CCNNNNNNNGG	4 cut(s)	888, 1053, 1133, 1244
AgsI	TTSAA	1 cut(s)	10
AjnI	CCWGG	6 cut(s)	565, 864, 1066, 1082, 1126, 1159
Alw21I	GWGCWC	1 cut(s)	266
AlwI	GGATC	1 cut(s)	382
AlwNI	CAGNNNCTG	2 cut(s)	641, 1053
Ama87I	CYCGRG	3 cut(s)	609, 886, 932
AoxI	GGCC	8 cut(s)	136, 637, 983, 993, 1064, 1140, 1157, 1264
ApeKI	GCWGC	2 cut(s)	413, 777
ArsI	GACNNNNNNTTYG	2 cut(s)	776, 808
Asp700I	GAANNNNTTC	1 cut(s)	405
AspLEI	GCGC	2 cut(s)	15, 186
AspS9I	GGNCC	5 cut(s)	638, 879, 1057, 1124, 1141
AsuC2I	CCSGG	4 cut(s)	887, 888, 933, 934
AsuHPI	GGTGA	3 cut(s)	574, 1024, 1162
AvaI	CYCGRG	3 cut(s)	609, 886, 932
AvaII	GGWCC	3 cut(s)	879, 1057, 1124
BalI	TGGCCA	1 cut(s)	1159
BbsI	GAAGAC	1 cut(s)	805
Bbv12I	GWGCWC	1 cut(s)	266
BbvCI	CCTCAGC	1 cut(s)	140
BbvI	GCAGC	2 cut(s)	425, 789
BccI	CCATC	5 cut(s)	365, 479, 500, 590, 638
BceAI	ACGGC	3 cut(s)	151, 794, 992
BciT130I	CCWGG	6 cut(s)	567, 866, 1068, 1084, 1128, 1161
BclI	TGATCA	1 cut(s)	679
BcnI	CCSGG	4 cut(s)	887, 888, 933, 934
BfaI	CTAG	4 cut(s)	17, 150, 540, 1002
BfmI	CTRYAG	5 cut(s)	694, 754, 919, 964, 1203
BfoI	RGCGCY	1 cut(s)	16
BisI	GCNGC	6 cut(s)	90, 126, 414, 778, 804, 807
BlpI	GCTNAGC	1 cut(s)	571
BlsI	GCNGC	6 cut(s)	91, 127, 415, 779, 805, 808
Bme18I	GGWCC	3 cut(s)	879, 1057, 1124
BmeT110I	CYCGRG	3 cut(s)	609, 886, 932
BmgT120I	GGNCC	5 cut(s)	638, 879, 1057, 1124, 1141
BmiI	GGNNCC	2 cut(s)	1058, 1125
BmrI	ACTGGG	1 cut(s)	1041
BmsI	GCATC	1 cut(s)	1005
BmuI	ACTGGG	1 cut(s)	1041
BpiI	GAAGAC	1 cut(s)	805
BpmI	CTGGAG	1 cut(s)	1155
Bpu10I	CCTNAGC	2 cut(s)	140, 658
Bpu1102I	GCTNAGC	1 cut(s)	571
BpuEI	CTTGAG	2 cut(s)	23, 1073
BpuMI	CCSGG	4 cut(s)	887, 888, 933, 934
BsaJI	CCNNGG	9 cut(s)	133, 886, 887, 932, 986, 1008, 1067, 1127, 1160
BsaWI	WCCGGW	1 cut(s)	318
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	4 cut(s)	888, 1053, 1133, 1244
Bse1I	ACTGG	3 cut(s)	1047, 1060, 1249
BseBI	CCWGG	6 cut(s)	567, 866, 1068, 1084, 1128, 1161
BseDI	CCNNGG	9 cut(s)	133, 886, 887, 932, 986, 1008, 1067, 1127, 1160
BseLI	CCNNNNNNNGG	4 cut(s)	888, 1053, 1133, 1244
BseMII	CTCAG	5 cut(s)	154, 178, 215, 585, 699
BseNI	ACTGG	3 cut(s)	1047, 1060, 1249
BseXI	GCAGC	2 cut(s)	425, 789
BseYI	CCCAGC	1 cut(s)	1053
Bsh1236I	CGCG	1 cut(s)	273
BshFI	GGCC	8 cut(s)	138, 639, 985, 995, 1066, 1142, 1159, 1266
BsiHKAI	GWGCWC	1 cut(s)	266
BsiHKCI	CYCGRG	3 cut(s)	609, 886, 932
BsiSI	CCGG	3 cut(s)	319, 887, 933
BslFI	GGGAC	1 cut(s)	1137
BslI	CCNNNNNNNGG	4 cut(s)	888, 1053, 1133, 1244
BsmFI	GGGAC	1 cut(s)	1137
BsnI	GGCC	8 cut(s)	138, 639, 985, 995, 1066, 1142, 1159, 1266
BsoBI	CYCGRG	3 cut(s)	609, 886, 932
Bsp1286I	GDGCHC	1 cut(s)	266
Bsp1407I	TGTACA	1 cut(s)	666
Bsp143I	GATC	2 cut(s)	374, 679
Bsp1720I	GCTNAGC	1 cut(s)	571
Bsp19I	CCATGG	1 cut(s)	986
Bsp68I	TCGCGA	1 cut(s)	273
BspACI	CCGC	9 cut(s)	90, 126, 449, 745, 804, 807, 838, 873, 1106
BspANI	GGCC	8 cut(s)	138, 639, 985, 995, 1066, 1142, 1159, 1266
BspCNI	CTCAG	5 cut(s)	153, 177, 214, 584, 698
BspFNI	CGCG	1 cut(s)	273
BspLI	GGNNCC	2 cut(s)	1058, 1125
BspPI	GGATC	1 cut(s)	382
BspQI	GCTCTTC	1 cut(s)	254
BsrGI	TGTACA	1 cut(s)	666
BsrI	ACTGG	3 cut(s)	1047, 1060, 1249
BssECI	CCNNGG	9 cut(s)	133, 886, 887, 932, 986, 1008, 1067, 1127, 1160
BssMI	GATC	2 cut(s)	374, 679
BssT1I	CCWWGG	1 cut(s)	986
Bst2UI	CCWGG	6 cut(s)	567, 866, 1068, 1084, 1128, 1161
Bst4CI	ACNGT	6 cut(s)	236, 394, 586, 920, 968, 1204
Bst6I	CTCTTC	2 cut(s)	254, 679
BstAPI	GCANNNNNTGC	1 cut(s)	362
BstAUI	TGTACA	1 cut(s)	666
BstC8I	GCNNGC	3 cut(s)	637, 842, 871
BstDEI	CTNAG	7 cut(s)	83, 140, 164, 201, 571, 658, 685
BstDSI	CCRYGG	3 cut(s)	133, 986, 1008
BstFNI	CGCG	1 cut(s)	273
BstH2I	RGCGCY	1 cut(s)	16
BstHHI	GCGC	2 cut(s)	15, 186
BstKTI	GATC	2 cut(s)	377, 682
BstMBI	GATC	2 cut(s)	374, 679
BstMWI	GCNNNNNNNGC	9 cut(s)	150, 270, 362, 410, 554, 711, 1112, 1139, 1187
BstNI	CCWGG	6 cut(s)	567, 866, 1068, 1084, 1128, 1161
BstSFI	CTRYAG	5 cut(s)	694, 754, 919, 964, 1203
BstUI	CGCG	1 cut(s)	273
BstV1I	GCAGC	2 cut(s)	425, 789
BstV2I	GAAGAC	1 cut(s)	805
BstXI	CCANNNNNNTGG	1 cut(s)	1067
BsuRI	GGCC	8 cut(s)	138, 639, 985, 995, 1066, 1142, 1159, 1266
BtgI	CCRYGG	3 cut(s)	133, 986, 1008
BtsI	GCAGTG	1 cut(s)	363
BtsIMutI	CAGTG	4 cut(s)	363, 1054, 1067, 1200
BtuMI	TCGCGA	1 cut(s)	273
Cac8I	GCNNGC	3 cut(s)	637, 842, 871
CaiI	CAGNNNCTG	2 cut(s)	641, 1053
CfoI	GCGC	2 cut(s)	15, 186
Cfr13I	GGNCC	5 cut(s)	638, 879, 1057, 1124, 1141
Cfr9I	CCCGGG	2 cut(s)	886, 932
CseI	GACGC	1 cut(s)	532
Csp6I	GTAC	3 cut(s)	252, 667, 1198
CviAII	CATG	6 cut(s)	78, 104, 773, 987, 1169, 1230
CviQI	GTAC	3 cut(s)	252, 667, 1198
DdeI	CTNAG	7 cut(s)	83, 140, 164, 201, 571, 658, 685
DpnI	GATC	2 cut(s)	376, 681
DpnII	GATC	2 cut(s)	374, 679
EaeI	YGGCCR	1 cut(s)	1157
Eam1104I	CTCTTC	2 cut(s)	254, 679
EarI	CTCTTC	2 cut(s)	254, 679
EciI	GGCGGA	1 cut(s)	734
Eco130I	CCWWGG	1 cut(s)	986
Eco147I	AGGCCT	1 cut(s)	995
Eco47I	GGWCC	3 cut(s)	879, 1057, 1124
Eco57I	CTGAAG	3 cut(s)	314, 402, 1095
Eco88I	CYCGRG	3 cut(s)	609, 886, 932
EcoO109I	RGGNCCY	2 cut(s)	638, 1124
EcoRII	CCWGG	6 cut(s)	565, 864, 1066, 1082, 1126, 1159
EcoT14I	CCWWGG	1 cut(s)	986
ErhI	CCWWGG	1 cut(s)	986
FaeI	CATG	6 cut(s)	81, 107, 776, 990, 1172, 1233
FalI	AAGNNNNNCTT	2 cut(s)	887, 919
FaqI	GGGAC	1 cut(s)	1137
FatI	CATG	6 cut(s)	77, 103, 772, 986, 1168, 1229
FauI	CCCGC	1 cut(s)	442
FbaI	TGATCA	1 cut(s)	679
FblI	GTMKAC	1 cut(s)	171
Fnu4HI	GCNGC	6 cut(s)	90, 126, 414, 778, 804, 807
Fsp4HI	GCNGC	6 cut(s)	90, 126, 414, 778, 804, 807
FspBI	CTAG	4 cut(s)	17, 150, 540, 1002
GlaI	GCGC	2 cut(s)	14, 185
GluI	GCNGC	6 cut(s)	90, 126, 414, 778, 804, 807
GsaI	CCCAGC	1 cut(s)	1057
GsuI	CTGGAG	1 cut(s)	1155
HaeII	RGCGCY	1 cut(s)	16
HaeIII	GGCC	8 cut(s)	138, 639, 985, 995, 1066, 1142, 1159, 1266
HapII	CCGG	3 cut(s)	319, 887, 933
HgaI	GACGC	1 cut(s)	532
HhaI	GCGC	2 cut(s)	15, 186
Hin1II	CATG	6 cut(s)	81, 107, 776, 990, 1172, 1233
Hin6I	GCGC	2 cut(s)	13, 184
HinP1I	GCGC	2 cut(s)	13, 184
HincII	GTYRAC	2 cut(s)	172, 232
HindII	GTYRAC	2 cut(s)	172, 232
HindIII	AAGCTT	1 cut(s)	1077
HinfI	GANTC	3 cut(s)	160, 536, 925
HpaI	GTTAAC	1 cut(s)	232
HpaII	CCGG	3 cut(s)	319, 887, 933
HphI	GGTGA	3 cut(s)	574, 1024, 1162
Hpy166II	GTNNAC	3 cut(s)	172, 232, 1200
Hpy188I	TCNGA	6 cut(s)	157, 400, 679, 797, 883, 940
Hpy188III	TCNNGA	5 cut(s)	40, 272, 611, 817, 913
Hpy8I	GTNNAC	3 cut(s)	172, 232, 1200
Hpy99I	CGWCG	1 cut(s)	548
HpyAV	CCTTC	4 cut(s)	439, 870, 985, 1248
HpyCH4III	ACNGT	6 cut(s)	236, 394, 586, 920, 968, 1204
HpyCH4IV	ACGT	2 cut(s)	110, 1119
HpyCH4V	TGCA	4 cut(s)	114, 365, 825, 1018
HpyF10VI	GCNNNNNNNGC	9 cut(s)	150, 270, 362, 410, 554, 711, 1112, 1139, 1187
HpyF3I	CTNAG	7 cut(s)	83, 140, 164, 201, 571, 658, 685
HpySE526I	ACGT	2 cut(s)	110, 1119
Hsp92II	CATG	6 cut(s)	81, 107, 776, 990, 1172, 1233
HspAI	GCGC	2 cut(s)	13, 184
Ksp22I	TGATCA	1 cut(s)	679
KspAI	GTTAAC	1 cut(s)	232
Kzo9I	GATC	2 cut(s)	374, 679
LguI	GCTCTTC	1 cut(s)	254
LmnI	GCTCC	3 cut(s)	158, 1048, 1178
Lsp1109I	GCAGC	2 cut(s)	425, 789
LweI	GCATC	1 cut(s)	1005
MaeI	CTAG	4 cut(s)	17, 150, 540, 1002
MaeII	ACGT	2 cut(s)	110, 1119
MaeIII	GTNAC	6 cut(s)	238, 586, 764, 1092, 1208, 1249
MalI	GATC	2 cut(s)	376, 681
MboI	GATC	2 cut(s)	374, 679
MboII	GAAGA	6 cut(s)	271, 325, 666, 784, 805, 826
MhlI	GDGCHC	1 cut(s)	266
MlsI	TGGCCA	1 cut(s)	1159
MluCI	AATT	2 cut(s)	267, 749
MluNI	TGGCCA	1 cut(s)	1159
MmeI	TCCRAC	3 cut(s)	378, 906, 1047
MnlI	CCTC	9 cut(s)	149, 155, 208, 280, 752, 791, 975, 1027, 1140
Mox20I	TGGCCA	1 cut(s)	1159
MroXI	GAANNNNTTC	1 cut(s)	405
MscI	TGGCCA	1 cut(s)	1159
MseI	TTAA	4 cut(s)	53, 231, 471, 954
Msp20I	TGGCCA	1 cut(s)	1159
MspI	CCGG	3 cut(s)	319, 887, 933
MvaI	CCWGG	6 cut(s)	567, 866, 1068, 1084, 1128, 1161
MvnI	CGCG	1 cut(s)	273
MwoI	GCNNNNNNNGC	9 cut(s)	150, 270, 362, 410, 554, 711, 1112, 1139, 1187
NciI	CCSGG	4 cut(s)	887, 888, 933, 934
NcoI	CCATGG	1 cut(s)	986
NdeII	GATC	2 cut(s)	374, 679
NlaIII	CATG	6 cut(s)	81, 107, 776, 990, 1172, 1233
NlaIV	GGNNCC	2 cut(s)	1058, 1125
NmuCI	GTSAC	3 cut(s)	764, 1092, 1249
NruI	TCGCGA	1 cut(s)	273
PaeR7I	CTCGAG	1 cut(s)	609
PceI	AGGCCT	1 cut(s)	995
PciSI	GCTCTTC	1 cut(s)	254
PcsI	WCGNNNNNNNCGW	2 cut(s)	807, 1005
PdmI	GAANNNNTTC	1 cut(s)	405
PfeI	GAWTC	3 cut(s)	160, 536, 925
PflFI	GACNNNGTC	1 cut(s)	394
PflMI	CCANNNNNTGG	1 cut(s)	1053
PkrI	GCNGC	6 cut(s)	91, 127, 415, 779, 805, 808
PpuMI	RGGWCCY	1 cut(s)	1124
PsiI	TTATAA	1 cut(s)	459
Psp5II	RGGWCCY	1 cut(s)	1124
Psp6I	CCWGG	6 cut(s)	565, 864, 1066, 1082, 1126, 1159
PspFI	CCCAGC	1 cut(s)	1053
PspGI	CCWGG	6 cut(s)	565, 864, 1066, 1082, 1126, 1159
PspN4I	GGNNCC	2 cut(s)	1058, 1125
PspPI	GGNCC	5 cut(s)	638, 879, 1057, 1124, 1141
PspPPI	RGGWCCY	1 cut(s)	1124
PstNI	CAGNNNCTG	2 cut(s)	641, 1053
PsyI	GACNNNGTC	1 cut(s)	394
RruI	TCGCGA	1 cut(s)	273
RsaI	GTAC	3 cut(s)	253, 668, 1199
RsaNI	GTAC	3 cut(s)	252, 667, 1198
SalI	GTCGAC	1 cut(s)	170
SapI	GCTCTTC	1 cut(s)	254
SaqAI	TTAA	4 cut(s)	53, 231, 471, 954
SatI	GCNGC	6 cut(s)	90, 126, 414, 778, 804, 807
Sau3AI	GATC	2 cut(s)	374, 679
Sau96I	GGNCC	5 cut(s)	638, 879, 1057, 1124, 1141
SduI	GDGCHC	1 cut(s)	266
SfaNI	GCATC	1 cut(s)	1005
SfcI	CTRYAG	5 cut(s)	694, 754, 919, 964, 1203
Sfr274I	CTCGAG	1 cut(s)	609
SinI	GGWCC	3 cut(s)	879, 1057, 1124
SlaI	CTCGAG	1 cut(s)	609
SmaI	CCCGGG	2 cut(s)	888, 934
SmlI	CTYRAG	3 cut(s)	38, 609, 1088
SmoI	CTYRAG	3 cut(s)	38, 609, 1088
Sse9I	AATT	2 cut(s)	267, 749
SseBI	AGGCCT	1 cut(s)	995
SsiI	CCGC	9 cut(s)	90, 126, 449, 745, 804, 807, 838, 873, 1106
SspMI	CTAG	4 cut(s)	17, 150, 540, 1002
StuI	AGGCCT	1 cut(s)	995
StyI	CCWWGG	1 cut(s)	986
TaaI	ACNGT	6 cut(s)	236, 394, 586, 920, 968, 1204
TaiI	ACGT	2 cut(s)	113, 1122
TaqI	TCGA	5 cut(s)	171, 518, 610, 783, 1241
TasI	AATT	2 cut(s)	267, 749
TatI	WGTACW	2 cut(s)	666, 1197
TauI	GCSGC	4 cut(s)	92, 128, 806, 809
TfiI	GAWTC	3 cut(s)	160, 536, 925
Tru1I	TTAA	4 cut(s)	53, 231, 471, 954
Tru9I	TTAA	4 cut(s)	53, 231, 471, 954
TscAI	CASTG	4 cut(s)	363, 1054, 1067, 1207
TseFI	GTSAC	3 cut(s)	764, 1092, 1249
TseI	GCWGC	2 cut(s)	413, 777
Tsp45I	GTSAC	3 cut(s)	764, 1092, 1249
TspDTI	ATGAA	7 cut(s)	120, 592, 761, 778, 1157, 1204, 1218
TspGWI	ACGGA	1 cut(s)	898
TspMI	CCCGGG	2 cut(s)	886, 932
TspRI	CASTG	4 cut(s)	363, 1054, 1067, 1207
Tth111I	GACNNNGTC	1 cut(s)	394
Van91I	CCANNNNNTGG	1 cut(s)	1053
VpaK11BI	GGWCC	3 cut(s)	879, 1057, 1124
XhoI	CTCGAG	1 cut(s)	609
XmaI	CCCGGG	2 cut(s)	886, 932
XmiI	GTMKAC	1 cut(s)	171
XmnI	GAANNNNTTC	1 cut(s)	405
XspI	CTAG	4 cut(s)	17, 150, 540, 1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.