Rroxscaffold_2G00145670

Pectinesterase

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
Physical Location & Seq
Forward (+)
83547878 .. 83604714
56837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145670.1

Sequence Viewer

Length: 1596 bp
ATGTTTCAGTTTGTGGTTTTATGGTTTCTTTGGGTACTGGGAGGGGCCTCAGTGTCTGGGGCTGCCTTGGAGAAGTACCAAGTGCATGTTAAAAAAGAATGCAGCTTTACTAGATATCCCAGCATTTGTGTTCAAACCTTGATGGGGTTGGGTGGTTCTGGGCAACAGGTTGATATAATGTCGGCTCTTGTTAACAAGACCATTTATGAGACCCGGTTGGCCACCTCCGAGTTTTCCCAATTCAGCTCCCAAGTATCTGAAGTCCGTGAAGCTCAACCCGCTTACCATTCTGTCAAAGACTACTGTGAAGAGTTAATGACCATGTCCTTGAAGCGCTTAGACCAATCCCTGATAGCTCTCAAGAAGTCTCCCTTGAAAAAAAAGCAAGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCAAGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGGTTGACCTTTCCAAGCGCATAGACTATGCCTCTCAGCTGGGAAGTAATCTATTAGCTCTTGTCAACCGTCTTACAAGTACCGATATGACGAAGAACACAACTCGCGATAAACAAGCTGAGGTTTTTCCCAAATGGGTTTCACCGAAGAACCGGAAACTACTTCAGGCAACCACCGTCAAAGCAAATGCGGTTGTTGCACAAGATGGATCAGGGAACTATAAGACTCTGTCGGAGGCTATAAATGCAGCTTCCGGGGGTCGATTTGTGATCTATGTGAAGTCAGGAGTTTATAAGGAGAAGATTAAGACTAATAAAGATGGTATTACGTTGATTGGAGATGGAAAATATTCAACTATTATTACTGGGGATTCTAGCGTCGCTAAAGGTGCTTCGATGCCTGGCTCAGCTACATTCACCGTAACAGGAGATGGGTTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAGGTGTAGCATTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTCTACAGAGAATGTGACATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTCATCTTCCGCAGGCACAAGGGCTATGGTGTTATCCTGGCAAGCGGGAGGTCCGACCCGGGGCAAAAGACGGGCTTCTCCGTCCAGAACTGTAGGATTCTGCCCGGGTCAGACTTATCTCCCGTTAAACATTCCTACAGTTCGTATCTTGGGAGGCCATGGAAGGCCTACGCTAGAGCCGTGGTGATGCAGTCCAACATAGACAACGTTATCGCTCCCAGTGGCTGGGTCCAGTGGCCTGGGGCTGAAGCTTCCCGGCTCAAGTCACTCTACTTAGCGGAGTTCGCAAACGTGGGACCTGGGGCTGGAGTGGCCCAGAGGGTGAAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAGTACACTGTAGGTAACTTTATTGGTGGAACTTCATGGCTTCCTTCGACTGGTGTGACTTTTGTTTCTGGCCTCCATTGA

Protein Analysis

531

Amino Acids

57.79

Weight (kDa)

9.12

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 183 1.4e-21 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 225 - 515 6.6e-113 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 1374
AccII CGCG 1 cut(s) 594
AclI AACGTT 1 cut(s) 1356
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 2 cut(s) 219, 1478
AcuI CTGAAG 3 cut(s) 279, 635, 1416
AfaI GTAC 5 cut(s) 36, 77, 568, 989, 1520
AfeI AGCGCT 1 cut(s) 335
AfiI CCNNNNNNNGG 5 cut(s) 144, 1209, 1374, 1454, 1565
AgsI TTSAA 4 cut(s) 134, 331, 376, 840
AjnI CCWGG 5 cut(s) 886, 1185, 1387, 1447, 1480
Alw26I GTCTC 2 cut(s) 203, 372
AlwI GGATC 1 cut(s) 703
AlwNI CAGNNNCTG 3 cut(s) 56, 962, 1374
Ama87I CYCGRG 3 cut(s) 930, 1207, 1253
Aor51HI AGCGCT 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 62, 102, 734, 1098
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
Asp700I GAANNNNTTC 1 cut(s) 835
AspLEI GCGC 2 cut(s) 336, 507
AspS9I GGNCC 6 cut(s) 45, 959, 1200, 1378, 1445, 1462
AsuC2I CCSGG 7 cut(s) 214, 742, 1208, 1209, 1254, 1255, 1405
AsuHPI GGTGA 4 cut(s) 621, 895, 1345, 1483
AvaI CYCGRG 3 cut(s) 930, 1207, 1253
AvaII GGWCC 3 cut(s) 1200, 1378, 1445
BalI TGGCCA 2 cut(s) 221, 1480
BbsI GAAGAC 1 cut(s) 1126
BbvCI CCTCAGC 2 cut(s) 461, 606
BbvI GCAGC 4 cut(s) 49, 114, 746, 1110
BccI CCATC 6 cut(s) 136, 686, 800, 821, 911, 959
BceAI ACGGC 3 cut(s) 472, 1115, 1313
BciT130I CCWGG 5 cut(s) 888, 1187, 1389, 1449, 1482
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 7 cut(s) 214, 742, 1208, 1209, 1254, 1255, 1405
BcoDI GTCTC 2 cut(s) 203, 372
BfaI CTAG 4 cut(s) 111, 471, 861, 1323
BfmI CTRYAG 5 cut(s) 1015, 1075, 1240, 1285, 1524
BfoI RGCGCY 1 cut(s) 337
BisI GCNGC 8 cut(s) 63, 103, 411, 447, 735, 1099, 1125, 1128
BlpI GCTNAGC 1 cut(s) 892
BlsI GCNGC 8 cut(s) 64, 104, 412, 448, 736, 1100, 1126, 1129
Bme18I GGWCC 3 cut(s) 1200, 1378, 1445
BmeT110I CYCGRG 3 cut(s) 930, 1207, 1253
BmgT120I GGNCC 6 cut(s) 45, 959, 1200, 1378, 1445, 1462
BmiI GGNNCC 3 cut(s) 46, 1379, 1446
BmrI ACTGGG 3 cut(s) 47, 861, 1362
BmsI GCATC 2 cut(s) 873, 1326
BmuI ACTGGG 3 cut(s) 47, 861, 1362
BpiI GAAGAC 1 cut(s) 1126
BpmI CTGGAG 1 cut(s) 1476
Bpu10I CCTNAGC 3 cut(s) 461, 606, 979
Bpu1102I GCTNAGC 1 cut(s) 892
BpuEI CTTGAG 2 cut(s) 344, 1394
BpuMI CCSGG 7 cut(s) 214, 742, 1208, 1209, 1254, 1255, 1405
BsaI GGTCTC 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 639
BsaXI ACNNNNNCTCC 2 cut(s) 1047, 1077
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 1209, 1374, 1454, 1565
Bse1I ACTGG 5 cut(s) 42, 856, 1368, 1381, 1570
BseBI CCWGG 5 cut(s) 888, 1187, 1389, 1449, 1482
BseLI CCNNNNNNNGG 5 cut(s) 144, 1209, 1374, 1454, 1565
BseMII CTCAG 7 cut(s) 63, 475, 499, 536, 597, 906, 1020
BseNI ACTGG 5 cut(s) 42, 856, 1368, 1381, 1570
BseXI GCAGC 4 cut(s) 49, 114, 746, 1110
BseYI CCCAGC 3 cut(s) 119, 526, 1374
Bsh1236I CGCG 1 cut(s) 594
BsiHKCI CYCGRG 3 cut(s) 930, 1207, 1253
BsiSI CCGG 6 cut(s) 214, 640, 741, 1208, 1254, 1405
BslFI GGGAC 1 cut(s) 1458
BslI CCNNNNNNNGG 5 cut(s) 144, 1209, 1374, 1454, 1565
BsmAI GTCTC 2 cut(s) 203, 372
BsmFI GGGAC 1 cut(s) 1458
BsmI GAATGC 1 cut(s) 104
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 3 cut(s) 930, 1207, 1253
Bsp1407I TGTACA 1 cut(s) 987
Bsp143I GATC 3 cut(s) 695, 756, 1000
Bsp1720I GCTNAGC 1 cut(s) 892
Bsp19I CCATGG 1 cut(s) 1307
Bsp68I TCGCGA 1 cut(s) 594
BspCNI CTCAG 7 cut(s) 62, 474, 498, 535, 598, 905, 1019
BspFNI CGCG 1 cut(s) 594
BspLI GGNNCC 3 cut(s) 46, 1379, 1446
BspPI GGATC 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 5 cut(s) 42, 856, 1368, 1381, 1570
BssMI GATC 3 cut(s) 695, 756, 1000
BssT1I CCWWGG 2 cut(s) 66, 1307
Bst2UI CCWGG 5 cut(s) 888, 1187, 1389, 1449, 1482
Bst4CI ACNGT 7 cut(s) 305, 557, 664, 907, 1241, 1289, 1525
Bst6I CTCTTC 2 cut(s) 303, 1000
BstAUI TGTACA 1 cut(s) 987
BstC8I GCNNGC 3 cut(s) 958, 1163, 1192
BstDSI CCRYGG 3 cut(s) 454, 1307, 1329
BstFNI CGCG 1 cut(s) 594
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 336, 507
BstKTI GATC 3 cut(s) 698, 759, 1003
BstMAI GTCTC 2 cut(s) 203, 372
BstMBI GATC 3 cut(s) 695, 756, 1000
BstMWI GCNNNNNNNGC 9 cut(s) 278, 471, 683, 731, 875, 1032, 1433, 1460, 1508
BstNI CCWGG 5 cut(s) 888, 1187, 1389, 1449, 1482
BstNSI RCATGY 1 cut(s) 89
BstSFI CTRYAG 5 cut(s) 1015, 1075, 1240, 1285, 1524
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 4 cut(s) 49, 114, 746, 1110
BstV2I GAAGAC 1 cut(s) 1126
BstXI CCANNNNNNTGG 1 cut(s) 1388
BtgI CCRYGG 3 cut(s) 454, 1307, 1329
BtsIMutI CAGTG 4 cut(s) 57, 1375, 1388, 1521
BtuMI TCGCGA 1 cut(s) 594
Cac8I GCNNGC 3 cut(s) 958, 1163, 1192
CaiI CAGNNNCTG 3 cut(s) 56, 962, 1374
CfoI GCGC 2 cut(s) 336, 507
Cfr13I GGNCC 6 cut(s) 45, 959, 1200, 1378, 1445, 1462
Cfr9I CCCGGG 2 cut(s) 1207, 1253
CseI GACGC 1 cut(s) 853
Csp6I GTAC 5 cut(s) 35, 76, 567, 988, 1519
CviAII CATG 7 cut(s) 86, 322, 399, 1094, 1308, 1490, 1551
CviQI GTAC 5 cut(s) 35, 76, 567, 988, 1519
DpnI GATC 3 cut(s) 697, 758, 1002
DpnII GATC 3 cut(s) 695, 756, 1000
EaeI YGGCCR 2 cut(s) 219, 1478
Eam1104I CTCTTC 2 cut(s) 303, 1000
EarI CTCTTC 2 cut(s) 303, 1000
EciI GGCGGA 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 66, 1307
Eco147I AGGCCT 1 cut(s) 1316
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 116
Eco47I GGWCC 3 cut(s) 1200, 1378, 1445
Eco47III AGCGCT 1 cut(s) 335
Eco57I CTGAAG 3 cut(s) 279, 635, 1416
Eco88I CYCGRG 3 cut(s) 930, 1207, 1253
EcoO109I RGGNCCY 3 cut(s) 45, 959, 1445
EcoRII CCWGG 5 cut(s) 886, 1185, 1387, 1447, 1480
EcoRV GATATC 1 cut(s) 116
EcoT14I CCWWGG 2 cut(s) 66, 1307
ErhI CCWWGG 2 cut(s) 66, 1307
FaeI CATG 7 cut(s) 89, 325, 402, 1097, 1311, 1493, 1554
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 1208, 1240
FaqI GGGAC 1 cut(s) 1458
FatI CATG 7 cut(s) 85, 321, 398, 1093, 1307, 1489, 1550
FauI CCCGC 2 cut(s) 286, 1187
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 8 cut(s) 63, 103, 411, 447, 735, 1099, 1125, 1128
Fsp4HI GCNGC 8 cut(s) 63, 103, 411, 447, 735, 1099, 1125, 1128
FspBI CTAG 4 cut(s) 111, 471, 861, 1323
GlaI GCGC 2 cut(s) 335, 506
GluI GCNGC 8 cut(s) 63, 103, 411, 447, 735, 1099, 1125, 1128
GsaI CCCAGC 3 cut(s) 123, 530, 1378
GsuI CTGGAG 1 cut(s) 1476
HaeII RGCGCY 1 cut(s) 337
HapII CCGG 6 cut(s) 214, 640, 741, 1208, 1254, 1405
HgaI GACGC 1 cut(s) 853
HhaI GCGC 2 cut(s) 336, 507
Hin1II CATG 7 cut(s) 89, 325, 402, 1097, 1311, 1493, 1554
Hin6I GCGC 2 cut(s) 334, 505
HinP1I GCGC 2 cut(s) 334, 505
HincII GTYRAC 3 cut(s) 193, 493, 553
HindII GTYRAC 3 cut(s) 193, 493, 553
HindIII AAGCTT 1 cut(s) 1398
HinfI GANTC 4 cut(s) 481, 712, 857, 1246
HpaI GTTAAC 1 cut(s) 193
HpaII CCGG 6 cut(s) 214, 640, 741, 1208, 1254, 1405
HphI GGTGA 4 cut(s) 621, 895, 1345, 1483
Hpy166II GTNNAC 4 cut(s) 193, 493, 553, 1521
Hpy188I TCNGA 8 cut(s) 229, 259, 478, 721, 1000, 1118, 1204, 1261
Hpy188III TCNNGA 6 cut(s) 361, 593, 771, 932, 1138, 1234
Hpy8I GTNNAC 4 cut(s) 193, 493, 553, 1521
Hpy99I CGWCG 1 cut(s) 869
HpyAV CCTTC 2 cut(s) 1306, 1569
HpyCH4III ACNGT 7 cut(s) 305, 557, 664, 907, 1241, 1289, 1525
HpyCH4IV ACGT 4 cut(s) 431, 815, 1356, 1440
HpyCH4V TGCA 7 cut(s) 85, 102, 435, 686, 734, 1146, 1339
HpyF10VI GCNNNNNNNGC 9 cut(s) 278, 471, 683, 731, 875, 1032, 1433, 1460, 1508
HpySE526I ACGT 4 cut(s) 431, 815, 1356, 1440
Hsp92II CATG 7 cut(s) 89, 325, 402, 1097, 1311, 1493, 1554
HspAI GCGC 2 cut(s) 334, 505
Ksp22I TGATCA 1 cut(s) 1000
KspAI GTTAAC 1 cut(s) 193
Kzo9I GATC 3 cut(s) 695, 756, 1000
LmnI GCTCC 4 cut(s) 251, 479, 1369, 1499
Lsp1109I GCAGC 4 cut(s) 49, 114, 746, 1110
LweI GCATC 2 cut(s) 873, 1326
MaeI CTAG 4 cut(s) 111, 471, 861, 1323
MaeII ACGT 4 cut(s) 431, 815, 1356, 1440
MaeIII GTNAC 5 cut(s) 907, 1085, 1413, 1529, 1570
MalI GATC 3 cut(s) 697, 758, 1002
MboI GATC 3 cut(s) 695, 756, 1000
MboII GAAGA 8 cut(s) 320, 592, 646, 799, 987, 1105, 1126, 1147
MlsI TGGCCA 2 cut(s) 221, 1480
MluCI AATT 2 cut(s) 239, 1070
MluNI TGGCCA 2 cut(s) 221, 1480
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 3 cut(s) 699, 1227, 1368
Mox20I TGGCCA 2 cut(s) 221, 1480
MroXI GAANNNNTTC 1 cut(s) 835
MscI TGGCCA 2 cut(s) 221, 1480
MseI TTAA 5 cut(s) 90, 192, 314, 792, 1275
Msp20I TGGCCA 2 cut(s) 221, 1480
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 6 cut(s) 214, 640, 741, 1208, 1254, 1405
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 5 cut(s) 888, 1187, 1389, 1449, 1482
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 9 cut(s) 278, 471, 683, 731, 875, 1032, 1433, 1460, 1508
NciI CCSGG 7 cut(s) 214, 742, 1208, 1209, 1254, 1255, 1405
NcoI CCATGG 1 cut(s) 1307
NdeII GATC 3 cut(s) 695, 756, 1000
NlaIII CATG 7 cut(s) 89, 325, 402, 1097, 1311, 1493, 1554
NlaIV GGNNCC 3 cut(s) 46, 1379, 1446
NmuCI GTSAC 3 cut(s) 1085, 1413, 1570
NruI TCGCGA 1 cut(s) 594
NspI RCATGY 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 930
PceI AGGCCT 1 cut(s) 1316
PcsI WCGNNNNNNNCGW 2 cut(s) 1128, 1326
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 835
PfeI GAWTC 3 cut(s) 481, 857, 1246
PflFI GACNNNGTC 2 cut(s) 322, 715
PflMI CCANNNNNTGG 1 cut(s) 1374
PkrI GCNGC 8 cut(s) 64, 104, 412, 448, 736, 1100, 1126, 1129
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PpuMI RGGWCCY 1 cut(s) 1445
PsiI TTATAA 1 cut(s) 780
Psp1406I AACGTT 1 cut(s) 1356
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 5 cut(s) 886, 1185, 1387, 1447, 1480
PspFI CCCAGC 3 cut(s) 119, 526, 1374
PspGI CCWGG 5 cut(s) 886, 1185, 1387, 1447, 1480
PspN4I GGNNCC 3 cut(s) 46, 1379, 1446
PspPI GGNCC 6 cut(s) 45, 959, 1200, 1378, 1445, 1462
PspPPI RGGWCCY 1 cut(s) 1445
PstNI CAGNNNCTG 3 cut(s) 56, 962, 1374
PsyI GACNNNGTC 2 cut(s) 322, 715
PvuII CAGCTG 1 cut(s) 526
RruI TCGCGA 1 cut(s) 594
RsaI GTAC 5 cut(s) 36, 77, 568, 989, 1520
RsaNI GTAC 5 cut(s) 35, 76, 567, 988, 1519
SaqAI TTAA 5 cut(s) 90, 192, 314, 792, 1275
SatI GCNGC 8 cut(s) 63, 103, 411, 447, 735, 1099, 1125, 1128
Sau3AI GATC 3 cut(s) 695, 756, 1000
Sau96I GGNCC 6 cut(s) 45, 959, 1200, 1378, 1445, 1462
SchI GAGTC 1 cut(s) 706
SfaNI GCATC 2 cut(s) 873, 1326
SfcI CTRYAG 5 cut(s) 1015, 1075, 1240, 1285, 1524
Sfr274I CTCGAG 1 cut(s) 930
SinI GGWCC 3 cut(s) 1200, 1378, 1445
SlaI CTCGAG 1 cut(s) 930
SmaI CCCGGG 2 cut(s) 1209, 1255
SmlI CTYRAG 3 cut(s) 359, 930, 1409
SmoI CTYRAG 3 cut(s) 359, 930, 1409
Sse9I AATT 2 cut(s) 239, 1070
SseBI AGGCCT 1 cut(s) 1316
SspI AATATT 1 cut(s) 836
SspMI CTAG 4 cut(s) 111, 471, 861, 1323
StuI AGGCCT 1 cut(s) 1316
StyI CCWWGG 2 cut(s) 66, 1307
TaaI ACNGT 7 cut(s) 305, 557, 664, 907, 1241, 1289, 1525
TaiI ACGT 4 cut(s) 434, 818, 1359, 1443
TaqI TCGA 5 cut(s) 748, 881, 931, 1104, 1562
TasI AATT 2 cut(s) 239, 1070
TatI WGTACW 2 cut(s) 987, 1518
TauI GCSGC 4 cut(s) 413, 449, 1127, 1130
TfiI GAWTC 3 cut(s) 481, 857, 1246
Tru1I TTAA 5 cut(s) 90, 192, 314, 792, 1275
Tru9I TTAA 5 cut(s) 90, 192, 314, 792, 1275
TscAI CASTG 4 cut(s) 57, 1375, 1388, 1528
TseFI GTSAC 3 cut(s) 1085, 1413, 1570
TseI GCWGC 4 cut(s) 62, 102, 734, 1098
Tsp45I GTSAC 3 cut(s) 1085, 1413, 1570
TspDTI ATGAA 5 cut(s) 913, 1082, 1099, 1478, 1539
TspGWI ACGGA 2 cut(s) 254, 1219
TspMI CCCGGG 2 cut(s) 1207, 1253
TspRI CASTG 4 cut(s) 57, 1375, 1388, 1528
Tth111I GACNNNGTC 2 cut(s) 322, 715
Van91I CCANNNNNTGG 1 cut(s) 1374
VpaK11BI GGWCC 3 cut(s) 1200, 1378, 1445
XceI RCATGY 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 930
XmaI CCCGGG 2 cut(s) 1207, 1253
XmnI GAANNNNTTC 1 cut(s) 835
XspI CTAG 4 cut(s) 111, 471, 861, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.