Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

7895127 .. 7905055

Gene Structure 9929 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G008050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGCCAGTTTGTTTGATACCTCTAAAATTTGAGAATATGTTTCAGTTTGTTGTTTTAGGGTTTCTTTGGGTATTGGGAGGGGCCTCAGAGTCTGGGGCTGCCTTGGATAAGTACCAAGTGTATGTTAAAAAGGAATGCAGCTTTACTAGATATCCCGGAATTTGTGTTCAAACCTTGATGGGGTTGGGTGGTTCTGGGCACAAGGATCCTCAGCAGGTTGATATAATGTCGGCTCTTGTTAACAAGACAATTTATGAAACCCGGTTCGCCACCTCCGAGTTTTCCCAATTCAGCTCTCAATTATTTCAAGTCCATGAAGCTCAACCCGCTCGCGATTCTGTCAAAGACCATTGTGAAGAGCTAATGACCATGTCTTTGAAGCGCCTAGACCAATGCCTAATAGCTCTCAAGAAATCTCCCTTGAAAAACAAGCACGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCATGAAACGTGCAAGGACTACGCCGCCGACCAGGGACTCAGCCTCACTAGCTCCGAGAATCTCAGGGTCGACCTTTCCAAGCGCATAGATTATGCCTCTCAGCTGGGAAGTAATCTATTAGCTCTCGTCAACCGTGTTACAAGTATCGGTACGCCGAAGAACACAATTCGCGATAAACAAGCAGAGGTTTTTCCCAAATGGGTTTCGCCGAAGAACCGGAAACTACTTCAGGCAACCACAGTCAAAGCGAACGCAGTGGTTGCACAAGATGGATCAGGGAACTATAAGACTGTGTCGGAGGCTATTCAGGCGGCTTCAGGGGGTCGGTTTGTGATTTATGTGAAGGCGGGAGTTTATAAGGAAAAGATTAAGACTAACAAAGATGGTATTACGTTGATTGGAGATGGAAAGTATTCAACTATTATTACTGGGGATTCTAGCGTCGCTAAAGGTGCTTCGACGCCTGGCTCAGCTACATTCACCGTAACAGGAGATGGGTTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAGGTGTAGCATTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTTTACAGAGAATGTGACATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTCATCTTCCGCAGGCACAAGGGCTATGGTGTTATCCTGGCAAGCGGGAGGTCCGACCCGGGGCAAAAGACGGGCTTCTCCGTCCAGAACTGTAGGATTCTGCCCGGGTCAGACTTATCTCCTGTTAAACATTCCTACAGTTCGTATCTTGGGAGGCCATGGAAGGACTATGCTAGAGCCGTGGTGATGCAATCCAACATAGGCGACGTTATCGCTCCCGGTGGCTGGGTCCAGTGGCCTGGGGCGAAAGCTTCCAGGCTCAAGTCACTCTACTTTGCGGAGTTCGCAAACGTGGGACCTGGGGCTGGAGTGGCCCAGAGGGTGAAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAAAAACAAAACTGCATGGTGACTTTGGCTAGTAATCTCAGGTTTAACTATCTCATACCAATCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

543

Amino Acids

59.31

Weight (kDa)

9.12

Isoelectric Point (pI)

34.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	41 - 199	7.9e-21	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	241 - 519	5.7e-112	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	828
Acc36I	ACCTGC	1 cut(s)	205
AccBSI	CCGCTC	1 cut(s)	329
AccI	GTMKAC	1 cut(s)	540
AccII	CGCG	2 cut(s)	333, 642
AclWI	GGATC	3 cut(s)	200, 213, 751
AcoI	YGGCCR	1 cut(s)	1526
AcsI	RAATTY	2 cut(s)	26, 159
AcuI	CTGAAG	2 cut(s)	683, 771
AcyI	GRCGYC	1 cut(s)	932
AfaI	GTAC	3 cut(s)	113, 622, 1037
AfiI	CCNNNNNNNGG	4 cut(s)	180, 1257, 1422, 1502
AgsI	TTSAA	6 cut(s)	170, 308, 379, 424, 888, 1565
AjnI	CCWGG	7 cut(s)	501, 934, 1233, 1435, 1451, 1495, 1528
AloI	GAACNNNNNNTCC	2 cut(s)	150, 182
AlwI	GGATC	3 cut(s)	200, 213, 751
AlwNI	CAGNNNCTG	2 cut(s)	92, 1010
Ama87I	CYCGRG	3 cut(s)	978, 1255, 1301
AoxI	GGCC	6 cut(s)	81, 1006, 1352, 1433, 1509, 1526
ApeKI	GCWGC	3 cut(s)	98, 138, 1146
ApoI	RAATTY	2 cut(s)	26, 159
ArsI	GACNNNNNNTTYG	4 cut(s)	358, 390, 1145, 1177
Asp700I	GAANNNNTTC	1 cut(s)	883
AspLEI	GCGC	2 cut(s)	384, 555
AspS9I	GGNCC	6 cut(s)	81, 1007, 1248, 1426, 1493, 1510
AsuC2I	CCSGG	7 cut(s)	156, 262, 1256, 1257, 1302, 1303, 1416
AsuHPI	GGTGA	4 cut(s)	943, 1393, 1531, 1594
AvaI	CYCGRG	3 cut(s)	978, 1255, 1301
AvaII	GGWCC	3 cut(s)	1248, 1426, 1493
BaeGI	GKGCMC	1 cut(s)	201
BalI	TGGCCA	1 cut(s)	1528
BamHI	GGATCC	1 cut(s)	205
BbsI	GAAGAC	1 cut(s)	1174
BbvCI	CCTCAGC	1 cut(s)	210
BbvI	GCAGC	3 cut(s)	85, 150, 1158
BccI	CCATC	6 cut(s)	172, 734, 848, 869, 959, 1007
BceAI	ACGGC	2 cut(s)	1163, 1361
BciT130I	CCWGG	7 cut(s)	503, 936, 1235, 1437, 1453, 1497, 1530
BclI	TGATCA	1 cut(s)	1048
BcnI	CCSGG	7 cut(s)	156, 262, 1256, 1257, 1302, 1303, 1416
BfaI	CTAG	6 cut(s)	147, 386, 519, 909, 1371, 1593
BfmI	CTRYAG	3 cut(s)	1063, 1288, 1333
BfoI	RGCGCY	1 cut(s)	385
BfuAI	ACCTGC	1 cut(s)	205
BisI	GCNGC	8 cut(s)	99, 139, 459, 495, 783, 1147, 1173, 1176
BlpI	GCTNAGC	1 cut(s)	940
BlsI	GCNGC	8 cut(s)	100, 140, 460, 496, 784, 1148, 1174, 1177
Bme18I	GGWCC	3 cut(s)	1248, 1426, 1493
BmeT110I	CYCGRG	3 cut(s)	978, 1255, 1301
BmgT120I	GGNCC	6 cut(s)	81, 1007, 1248, 1426, 1493, 1510
BmiI	GGNNCC	4 cut(s)	82, 207, 1427, 1494
BmrI	ACTGGG	1 cut(s)	909
BmsI	GCATC	1 cut(s)	1374
BmuI	ACTGGG	1 cut(s)	909
BpiI	GAAGAC	1 cut(s)	1174
BpmI	CTGGAG	1 cut(s)	1524
Bpu10I	CCTNAGC	2 cut(s)	210, 1027
Bpu1102I	GCTNAGC	1 cut(s)	940
BpuEI	CTTGAG	2 cut(s)	392, 1442
BpuMI	CCSGG	7 cut(s)	156, 262, 1256, 1257, 1302, 1303, 1416
BsaHI	GRCGYC	1 cut(s)	932
BsaWI	WCCGGW	1 cut(s)	687
BsaXI	ACNNNNNCTCC	2 cut(s)	1095, 1125
Bsc4I	CCNNNNNNNGG	4 cut(s)	180, 1257, 1422, 1502
Bse1I	ACTGG	4 cut(s)	5, 904, 1429, 1627
BseBI	CCWGG	7 cut(s)	503, 936, 1235, 1437, 1453, 1497, 1530
BseLI	CCNNNNNNNGG	4 cut(s)	180, 1257, 1422, 1502
BseMII	CTCAG	8 cut(s)	99, 224, 523, 547, 584, 954, 1068, 1615
BseNI	ACTGG	4 cut(s)	5, 904, 1429, 1627
BseSI	GKGCMC	1 cut(s)	201
BseXI	GCAGC	3 cut(s)	85, 150, 1158
BseYI	CCCAGC	2 cut(s)	574, 1422
Bsh1236I	CGCG	2 cut(s)	333, 642
BshFI	GGCC	6 cut(s)	83, 1008, 1354, 1435, 1511, 1528
BsiHKCI	CYCGRG	3 cut(s)	978, 1255, 1301
BsiSI	CCGG	6 cut(s)	156, 262, 688, 1256, 1302, 1416
BslFI	GGGAC	2 cut(s)	519, 1506
BslI	CCNNNNNNNGG	4 cut(s)	180, 1257, 1422, 1502
BsmFI	GGGAC	2 cut(s)	519, 1506
BsmI	GAATGC	1 cut(s)	140
BsnI	GGCC	6 cut(s)	83, 1008, 1354, 1435, 1511, 1528
BsoBI	CYCGRG	3 cut(s)	978, 1255, 1301
Bsp1286I	GDGCHC	1 cut(s)	201
Bsp1407I	TGTACA	1 cut(s)	1035
Bsp143I	GATC	3 cut(s)	205, 743, 1048
Bsp1720I	GCTNAGC	1 cut(s)	940
Bsp19I	CCATGG	1 cut(s)	1355
Bsp68I	TCGCGA	2 cut(s)	333, 642
BspANI	GGCC	6 cut(s)	83, 1008, 1354, 1435, 1511, 1528
BspCNI	CTCAG	8 cut(s)	98, 223, 522, 546, 583, 953, 1067, 1614
BspFNI	CGCG	2 cut(s)	333, 642
BspLI	GGNNCC	4 cut(s)	82, 207, 1427, 1494
BspMI	ACCTGC	1 cut(s)	205
BspPI	GGATC	3 cut(s)	200, 213, 751
BspQI	GCTCTTC	1 cut(s)	351
BsrBI	CCGCTC	1 cut(s)	329
BsrGI	TGTACA	1 cut(s)	1035
BsrI	ACTGG	4 cut(s)	5, 904, 1429, 1627
BssMI	GATC	3 cut(s)	205, 743, 1048
BssNI	GRCGYC	1 cut(s)	932
BssT1I	CCWWGG	2 cut(s)	102, 1355
Bst2UI	CCWGG	7 cut(s)	503, 936, 1235, 1437, 1453, 1497, 1530
Bst4CI	ACNGT	6 cut(s)	605, 712, 763, 955, 1289, 1337
Bst6I	CTCTTC	2 cut(s)	351, 1048
BstACI	GRCGYC	1 cut(s)	932
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstAUI	TGTACA	1 cut(s)	1035
BstC8I	GCNNGC	4 cut(s)	331, 1006, 1211, 1240
BstDSI	CCRYGG	2 cut(s)	1355, 1377
BstFNI	CGCG	2 cut(s)	333, 642
BstH2I	RGCGCY	1 cut(s)	385
BstHHI	GCGC	2 cut(s)	384, 555
BstKTI	GATC	3 cut(s)	208, 746, 1051
BstMBI	GATC	3 cut(s)	205, 743, 1048
BstMWI	GCNNNNNNNGC	9 cut(s)	326, 519, 731, 779, 923, 1080, 1481, 1508, 1556
BstNI	CCWGG	7 cut(s)	503, 936, 1235, 1437, 1453, 1497, 1530
BstSFI	CTRYAG	3 cut(s)	1063, 1288, 1333
BstSLI	GKGCMC	1 cut(s)	201
BstUI	CGCG	2 cut(s)	333, 642
BstV1I	GCAGC	3 cut(s)	85, 150, 1158
BstV2I	GAAGAC	1 cut(s)	1174
BstX2I	RGATCY	1 cut(s)	205
BstXI	CCANNNNNNTGG	1 cut(s)	1436
BstYI	RGATCY	1 cut(s)	205
BsuRI	GGCC	6 cut(s)	83, 1008, 1354, 1435, 1511, 1528
BtgI	CCRYGG	2 cut(s)	1355, 1377
BtsI	GCAGTG	1 cut(s)	732
BtsIMutI	CAGTG	2 cut(s)	732, 1436
BtuMI	TCGCGA	2 cut(s)	333, 642
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	4 cut(s)	331, 1006, 1211, 1240
CaiI	CAGNNNCTG	2 cut(s)	92, 1010
CfoI	GCGC	2 cut(s)	384, 555
Cfr13I	GGNCC	6 cut(s)	81, 1007, 1248, 1426, 1493, 1510
Cfr9I	CCCGGG	2 cut(s)	1255, 1301
CseI	GACGC	2 cut(s)	901, 940
Csp6I	GTAC	3 cut(s)	112, 621, 1036
CviAII	CATG	8 cut(s)	314, 370, 447, 473, 1142, 1356, 1538, 1579
CviQI	GTAC	3 cut(s)	112, 621, 1036
DpnI	GATC	3 cut(s)	207, 745, 1050
DpnII	GATC	3 cut(s)	205, 743, 1048
EaeI	YGGCCR	1 cut(s)	1526
Eam1104I	CTCTTC	2 cut(s)	351, 1048
EarI	CTCTTC	2 cut(s)	351, 1048
EciI	GGCGGA	1 cut(s)	1103
Eco130I	CCWWGG	2 cut(s)	102, 1355
Eco32I	GATATC	1 cut(s)	152
Eco47I	GGWCC	3 cut(s)	1248, 1426, 1493
Eco57I	CTGAAG	2 cut(s)	683, 771
Eco88I	CYCGRG	3 cut(s)	978, 1255, 1301
EcoO109I	RGGNCCY	3 cut(s)	81, 1007, 1493
EcoRII	CCWGG	7 cut(s)	501, 934, 1233, 1435, 1451, 1495, 1528
EcoRV	GATATC	1 cut(s)	152
EcoT14I	CCWWGG	2 cut(s)	102, 1355
ErhI	CCWWGG	2 cut(s)	102, 1355
FaeI	CATG	8 cut(s)	317, 373, 450, 476, 1145, 1359, 1541, 1582
FalI	AAGNNNNNCTT	2 cut(s)	1256, 1288
FaqI	GGGAC	2 cut(s)	519, 1506
FatI	CATG	8 cut(s)	313, 369, 446, 472, 1141, 1355, 1537, 1578
FauI	CCCGC	3 cut(s)	334, 811, 1235
FbaI	TGATCA	1 cut(s)	1048
FblI	GTMKAC	1 cut(s)	540
Fnu4HI	GCNGC	8 cut(s)	99, 139, 459, 495, 783, 1147, 1173, 1176
Fsp4HI	GCNGC	8 cut(s)	99, 139, 459, 495, 783, 1147, 1173, 1176
FspBI	CTAG	6 cut(s)	147, 386, 519, 909, 1371, 1593
GlaI	GCGC	2 cut(s)	383, 554
GluI	GCNGC	8 cut(s)	99, 139, 459, 495, 783, 1147, 1173, 1176
GsaI	CCCAGC	2 cut(s)	578, 1426
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	1 cut(s)	385
HaeIII	GGCC	6 cut(s)	83, 1008, 1354, 1435, 1511, 1528
HapII	CCGG	6 cut(s)	156, 262, 688, 1256, 1302, 1416
HgaI	GACGC	2 cut(s)	901, 940
HhaI	GCGC	2 cut(s)	384, 555
Hin1I	GRCGYC	1 cut(s)	932
Hin1II	CATG	8 cut(s)	317, 373, 450, 476, 1145, 1359, 1541, 1582
Hin6I	GCGC	2 cut(s)	382, 553
HinP1I	GCGC	2 cut(s)	382, 553
HincII	GTYRAC	3 cut(s)	241, 541, 601
HindII	GTYRAC	3 cut(s)	241, 541, 601
HindIII	AAGCTT	1 cut(s)	1446
HinfI	GANTC	6 cut(s)	89, 335, 507, 529, 905, 1294
HpaI	GTTAAC	1 cut(s)	241
HpaII	CCGG	6 cut(s)	156, 262, 688, 1256, 1302, 1416
HphI	GGTGA	4 cut(s)	943, 1393, 1531, 1594
Hpy166II	GTNNAC	3 cut(s)	241, 541, 601
Hpy188I	TCNGA	8 cut(s)	88, 277, 526, 769, 1048, 1166, 1252, 1309
Hpy188III	TCNNGA	6 cut(s)	332, 409, 641, 980, 1186, 1282
Hpy8I	GTNNAC	3 cut(s)	241, 541, 601
Hpy99I	CGWCG	3 cut(s)	917, 934, 1406
HpyAV	CCTTC	2 cut(s)	808, 1354
HpyCH4III	ACNGT	6 cut(s)	605, 712, 763, 955, 1289, 1337
HpyCH4IV	ACGT	4 cut(s)	479, 863, 1404, 1488
HpyCH4V	TGCA	6 cut(s)	138, 483, 734, 1194, 1387, 1578
HpyF10VI	GCNNNNNNNGC	9 cut(s)	326, 519, 731, 779, 923, 1080, 1481, 1508, 1556
HpySE526I	ACGT	4 cut(s)	479, 863, 1404, 1488
Hsp92I	GRCGYC	1 cut(s)	932
Hsp92II	CATG	8 cut(s)	317, 373, 450, 476, 1145, 1359, 1541, 1582
HspAI	GCGC	2 cut(s)	382, 553
Ksp22I	TGATCA	1 cut(s)	1048
KspAI	GTTAAC	1 cut(s)	241
Kzo9I	GATC	3 cut(s)	205, 743, 1048
LguI	GCTCTTC	1 cut(s)	351
LmnI	GCTCC	3 cut(s)	527, 1417, 1547
Lsp1109I	GCAGC	3 cut(s)	85, 150, 1158
LweI	GCATC	1 cut(s)	1374
MaeI	CTAG	6 cut(s)	147, 386, 519, 909, 1371, 1593
MaeII	ACGT	4 cut(s)	479, 863, 1404, 1488
MaeIII	GTNAC	5 cut(s)	607, 955, 1133, 1461, 1582
MalI	GATC	3 cut(s)	207, 745, 1050
MbiI	CCGCTC	1 cut(s)	329
MboI	GATC	3 cut(s)	205, 743, 1048
MboII	GAAGA	7 cut(s)	368, 640, 694, 1035, 1153, 1174, 1195
MflI	RGATCY	1 cut(s)	205
MhlI	GDGCHC	1 cut(s)	201
MlsI	TGGCCA	1 cut(s)	1528
MluCI	AATT	7 cut(s)	26, 159, 249, 287, 299, 636, 1118
MluNI	TGGCCA	1 cut(s)	1528
MlyI	GAGTC	2 cut(s)	98, 501
MmeI	TCCRAC	3 cut(s)	747, 1275, 1416
Mox20I	TGGCCA	1 cut(s)	1528
MroXI	GAANNNNTTC	1 cut(s)	883
MscI	TGGCCA	1 cut(s)	1528
MseI	TTAA	5 cut(s)	126, 240, 840, 1323, 1608
Msp20I	TGGCCA	1 cut(s)	1528
MspA1I	CMGCKG	1 cut(s)	574
MspI	CCGG	6 cut(s)	156, 262, 688, 1256, 1302, 1416
Mva1269I	GAATGC	1 cut(s)	140
MvaI	CCWGG	7 cut(s)	503, 936, 1235, 1437, 1453, 1497, 1530
MvnI	CGCG	2 cut(s)	333, 642
MwoI	GCNNNNNNNGC	9 cut(s)	326, 519, 731, 779, 923, 1080, 1481, 1508, 1556
NciI	CCSGG	7 cut(s)	156, 262, 1256, 1257, 1302, 1303, 1416
NcoI	CCATGG	1 cut(s)	1355
NdeII	GATC	3 cut(s)	205, 743, 1048
NlaIII	CATG	8 cut(s)	317, 373, 450, 476, 1145, 1359, 1541, 1582
NlaIV	GGNNCC	4 cut(s)	82, 207, 1427, 1494
NmuCI	GTSAC	3 cut(s)	1133, 1461, 1582
NruI	TCGCGA	2 cut(s)	333, 642
PaeR7I	CTCGAG	1 cut(s)	978
PciSI	GCTCTTC	1 cut(s)	351
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 1176
PctI	GAATGC	1 cut(s)	140
PdmI	GAANNNNTTC	1 cut(s)	883
PfeI	GAWTC	4 cut(s)	335, 529, 905, 1294
PflFI	GACNNNGTC	2 cut(s)	370, 763
PfoI	TCCNGGA	1 cut(s)	154
PkrI	GCNGC	8 cut(s)	100, 140, 460, 496, 784, 1148, 1174, 1177
PleI	GAGTC	2 cut(s)	97, 501
PpsI	GAGTC	2 cut(s)	97, 501
PpuMI	RGGWCCY	1 cut(s)	1493
PsiI	TTATAA	1 cut(s)	828
Psp5II	RGGWCCY	1 cut(s)	1493
Psp6I	CCWGG	7 cut(s)	501, 934, 1233, 1435, 1451, 1495, 1528
PspFI	CCCAGC	2 cut(s)	574, 1422
PspGI	CCWGG	7 cut(s)	501, 934, 1233, 1435, 1451, 1495, 1528
PspN4I	GGNNCC	4 cut(s)	82, 207, 1427, 1494
PspPI	GGNCC	6 cut(s)	81, 1007, 1248, 1426, 1493, 1510
PspPPI	RGGWCCY	1 cut(s)	1493
PstNI	CAGNNNCTG	2 cut(s)	92, 1010
PsuI	RGATCY	1 cut(s)	205
PsyI	GACNNNGTC	2 cut(s)	370, 763
PvuII	CAGCTG	1 cut(s)	574
RruI	TCGCGA	2 cut(s)	333, 642
RsaI	GTAC	3 cut(s)	113, 622, 1037
RsaNI	GTAC	3 cut(s)	112, 621, 1036
SalI	GTCGAC	1 cut(s)	539
SapI	GCTCTTC	1 cut(s)	351
SaqAI	TTAA	5 cut(s)	126, 240, 840, 1323, 1608
SatI	GCNGC	8 cut(s)	99, 139, 459, 495, 783, 1147, 1173, 1176
Sau3AI	GATC	3 cut(s)	205, 743, 1048
Sau96I	GGNCC	6 cut(s)	81, 1007, 1248, 1426, 1493, 1510
SchI	GAGTC	2 cut(s)	98, 501
SduI	GDGCHC	1 cut(s)	201
SfaNI	GCATC	1 cut(s)	1374
SfcI	CTRYAG	3 cut(s)	1063, 1288, 1333
Sfr274I	CTCGAG	1 cut(s)	978
SinI	GGWCC	3 cut(s)	1248, 1426, 1493
SlaI	CTCGAG	1 cut(s)	978
SmaI	CCCGGG	2 cut(s)	1257, 1303
SmlI	CTYRAG	3 cut(s)	407, 978, 1457
SmoI	CTYRAG	3 cut(s)	407, 978, 1457
Sse9I	AATT	7 cut(s)	26, 159, 249, 287, 299, 636, 1118
SspMI	CTAG	6 cut(s)	147, 386, 519, 909, 1371, 1593
StyI	CCWWGG	2 cut(s)	102, 1355
TaaI	ACNGT	6 cut(s)	605, 712, 763, 955, 1289, 1337
TaiI	ACGT	4 cut(s)	482, 866, 1407, 1491
TaqI	TCGA	4 cut(s)	540, 929, 979, 1152
TasI	AATT	7 cut(s)	26, 159, 249, 287, 299, 636, 1118
TatI	WGTACW	1 cut(s)	1035
TauI	GCSGC	5 cut(s)	461, 497, 785, 1175, 1178
TfiI	GAWTC	4 cut(s)	335, 529, 905, 1294
Tru1I	TTAA	5 cut(s)	126, 240, 840, 1323, 1608
Tru9I	TTAA	5 cut(s)	126, 240, 840, 1323, 1608
TscAI	CASTG	2 cut(s)	732, 1436
TseFI	GTSAC	3 cut(s)	1133, 1461, 1582
TseI	GCWGC	3 cut(s)	98, 138, 1146
Tsp45I	GTSAC	3 cut(s)	1133, 1461, 1582
TspDTI	ATGAA	7 cut(s)	270, 330, 489, 961, 1130, 1147, 1526
TspGWI	ACGGA	1 cut(s)	1267
TspMI	CCCGGG	2 cut(s)	1255, 1301
TspRI	CASTG	2 cut(s)	732, 1436
Tth111I	GACNNNGTC	2 cut(s)	370, 763
VpaK11BI	GGWCC	3 cut(s)	1248, 1426, 1493
XapI	RAATTY	2 cut(s)	26, 159
XhoI	CTCGAG	1 cut(s)	978
XmaI	CCCGGG	2 cut(s)	1255, 1301
XmiI	GTMKAC	1 cut(s)	540
XmnI	GAANNNNTTC	1 cut(s)	883
XspI	CTAG	6 cut(s)	147, 386, 519, 909, 1371, 1593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.