Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009155

Physical Location & Seq

Genomic Coordinates Reverse (-)

7975390 .. 7979414

Gene Structure 4025 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G008060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGTTTCAGTTTGTGGTTTTAGGGTTTCTTTGGGTACTGGGAGGGGCCTCAGTGTCTGGGGCTGCCTTGGAGAAATACCAAGTGCATGTTAAAAAAGAATGCAGCTTTACTAGATATCCCAGCATTTGTGTTCAAACCTTGATGGGATTGGGTGGTTCTGGGCAACAGGTTGATATAATGTCGGCTCTTGTCAACAAGACCATTTATGAGACCCGGTTGGCCACCTCCGAGTTTTCCCAATTCAGCTCCCAAGTATCTGAAGTCCGTGAAGCTCAACCCGCTCACCATTCTGTCAAAGACTACTGTGAAGAGTTAATGACCATGTCCTTGAAGCGCTTAGACCAATCCCTGATAGCTCTGAAGAAGTCTCCCTTGAAAAAAAAGCAAGACATTCAGGCATGGCTAAGTGCCGCCTTGACTTTCCAAGAAACGTGCAAGGACTACGCCGCCGACCACGGCCTCAGCCTCACTAGCTCCGAGAATCTCAGGGTCGACCTTTCCAAGCGCATAGATTATGCCTCTCAGTTGGGAAGTAATCTATTAGCTCTCGTCAACCGTGTTACAAGTATCGGTACGCCGAAGAACACAATTCGCGATAAACAAGCAGAGGTTTTTCCCAAATGGGTTTCGCCGAAGAACCGGAAACTACTTCAGGCAACCACAGTCAAAGCGAACGCAGTGGTTGCACAAGATGGATCAGGGAACTATAAGACTGTGTCGGAGGCTATTCAGGCAGCTACAGGGGGTCGGTTTGTGATTTATGTGAAGGCGGGAGTTTATAAGGAAAAGATTAAGACTAACAAAGATGGTATTACGTTGATTGGAGATGGAAAGTATTCGACTATTATTACTGGGGATTCTAGCGTCGCTAAAGGTGCTTCGATGCCTGGCTCAGCTACATTCACCGTAACAGGAGATGGGTTCATAGCTCGAGATATTGGTTTCCAAAACACAGCAGGCCCTGATGGAGAACAAGCCTTAGCTCTGTACATCTCTTCTGATCACTCAGTTCTCTACAAGTGTAGCATTGTTGGCTACCAAGACACACTCTACGCACTCGCCCTCCGCCAATTCTACAGAGAATGTGATATTCATGGCAGCCTCGACTTCATCTTCGGAAACGCCGCCGCCGTCTTCCAGAGTTGCAATCTCATCTTGCGCAGGCACAAGGGCTATGGTGCTATCCTGGCAAGCGGGAGGTCCGACCCAGGGCAAAAGACGGGCTTCTCCGTCCAGAACTGTAGGATTGTGCCCGGGTCAGACTTATCTCCTGTTAAACATTCCTACAGTTCGTATCTTGGGAGACCATGGAAGGCCTACGCTAGAGCCGTGGTGATGCAGTCCAACATAGACGAGATTATCGCTCCCAGAGGCTGGGTCGAGTGGCCTGGGGCTGGAGGTTCCAGCCTCAAGTCACTCTACTTTGCGGAGTTCACAAACATGGGGCCTGGGGCTGGAGTGGCCCAGAGGGTGCAGTGGCCAGGGTTTCATGTTATTGGAGCTGATATTGCTGTTGAGTACACTGTAGGTAACTTTATTGGTGGAACTTCATGGCTGCCTTCGACTGGTGTGGCTTTTGTTTCTGGCCTCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

531

Amino Acids

57.46

Weight (kDa)

9.07

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	30 - 183	1.3e-21	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	225 - 515	7.6e-114	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20860 AT5G20860
fragaria_vesca	FvH4_1g09560
malus_domestica	MD02G1104200.v1.1
prunus_persica	Prupe.7G190700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0096331 RchiOBHm_Chr2g0096371
rosa_laevigata	RLG00000016634 RLG00000016637
rosa_multiflora	Rmu_sc0004244.1_g000006 Rmu_sc0005996.1_g000024 Rmu_sc0005996.1_g000034
rosa_roxburghii	Rroxscaffold_2G00145670
rosa_rugosa	Rorug02G0057600 Rorug02G0057700 Rorug02G0057700
rosa_samantha	Rh2AG104500 Rh2BG106600 Rh2CG108500 Rh2DG107400
rosa_wichuraiana	Rw2G008050 Rw2G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
Acc16I	TGCGCA	1 cut(s)	1160
AccB7I	CCANNNNNTGG	1 cut(s)	1374
AccBSI	CCGCTC	1 cut(s)	281
AccI	GTMKAC	1 cut(s)	492
AccII	CGCG	1 cut(s)	594
AciI	CCGC	9 cut(s)	279, 411, 447, 770, 1066, 1125, 1128, 1194, 1427
AclWI	GGATC	1 cut(s)	703
AcoI	YGGCCR	2 cut(s)	219, 1478
AcuI	CTGAAG	3 cut(s)	279, 380, 635
AfaI	GTAC	4 cut(s)	36, 574, 989, 1520
AfeI	AGCGCT	1 cut(s)	335
AfiI	CCNNNNNNNGG	5 cut(s)	1209, 1374, 1394, 1454, 1565
AgsI	TTSAA	3 cut(s)	134, 331, 376
AjnI	CCWGG	6 cut(s)	886, 1185, 1207, 1387, 1447, 1480
Alw26I	GTCTC	3 cut(s)	203, 372, 1297
AlwI	GGATC	1 cut(s)	703
AlwNI	CAGNNNCTG	3 cut(s)	56, 962, 1374
Ama87I	CYCGRG	2 cut(s)	930, 1253
Aor51HI	AGCGCT	1 cut(s)	335
ApeKI	GCWGC	5 cut(s)	62, 102, 734, 1098, 1555
ArsI	GACNNNNNNTTYG	2 cut(s)	1097, 1129
Asp700I	GAANNNNTTC	1 cut(s)	835
AspLEI	GCGC	3 cut(s)	336, 507, 1161
AspS9I	GGNCC	5 cut(s)	45, 959, 1200, 1445, 1462
AsuC2I	CCSGG	3 cut(s)	214, 1254, 1255
AsuHPI	GGTGA	3 cut(s)	275, 895, 1345
AvaI	CYCGRG	2 cut(s)	930, 1253
AvaII	GGWCC	1 cut(s)	1200
BaeGI	GKGCMC	1 cut(s)	1254
BalI	TGGCCA	2 cut(s)	221, 1480
BbsI	GAAGAC	1 cut(s)	1126
BbvCI	CCTCAGC	1 cut(s)	461
BbvI	GCAGC	5 cut(s)	49, 114, 746, 1110, 1542
BccI	CCATC	6 cut(s)	136, 686, 800, 821, 911, 959
BceAI	ACGGC	3 cut(s)	472, 1115, 1313
BciT130I	CCWGG	6 cut(s)	888, 1187, 1209, 1389, 1449, 1482
BclI	TGATCA	1 cut(s)	1000
BcnI	CCSGG	3 cut(s)	214, 1254, 1255
BcoDI	GTCTC	3 cut(s)	203, 372, 1297
BfaI	CTAG	4 cut(s)	111, 471, 861, 1323
BfmI	CTRYAG	5 cut(s)	738, 1075, 1240, 1285, 1524
BfoI	RGCGCY	1 cut(s)	337
BisI	GCNGC	9 cut(s)	63, 103, 411, 447, 735, 1099, 1125, 1128, 1556
BlpI	GCTNAGC	1 cut(s)	892
BlsI	GCNGC	9 cut(s)	64, 104, 412, 448, 736, 1100, 1126, 1129, 1557
Bme1390I	CCNGG	9 cut(s)	214, 888, 1187, 1209, 1254, 1255, 1389, 1449, 1482
Bme18I	GGWCC	1 cut(s)	1200
BmeT110I	CYCGRG	2 cut(s)	930, 1253
BmgT120I	GGNCC	5 cut(s)	45, 959, 1200, 1445, 1462
BmiI	GGNNCC	3 cut(s)	46, 1402, 1446
BmrFI	CCNGG	9 cut(s)	214, 888, 1187, 1209, 1254, 1255, 1389, 1449, 1482
BmrI	ACTGGG	2 cut(s)	47, 861
BmsI	GCATC	2 cut(s)	873, 1326
BmuI	ACTGGG	2 cut(s)	47, 861
BpiI	GAAGAC	1 cut(s)	1126
BpmI	CTGGAG	2 cut(s)	1416, 1476
Bpu10I	CCTNAGC	2 cut(s)	461, 979
Bpu1102I	GCTNAGC	1 cut(s)	892
BpuEI	CTTGAG	1 cut(s)	1394
BpuMI	CCSGG	3 cut(s)	214, 1254, 1255
BsaI	GGTCTC	2 cut(s)	203, 1297
BsaWI	WCCGGW	1 cut(s)	639
BsaXI	ACNNNNNCTCC	2 cut(s)	1047, 1077
Bsc4I	CCNNNNNNNGG	5 cut(s)	1209, 1374, 1394, 1454, 1565
Bse1I	ACTGG	3 cut(s)	42, 856, 1570
BseBI	CCWGG	6 cut(s)	888, 1187, 1209, 1389, 1449, 1482
BseLI	CCNNNNNNNGG	5 cut(s)	1209, 1374, 1394, 1454, 1565
BseMII	CTCAG	6 cut(s)	63, 475, 499, 536, 906, 1020
BseNI	ACTGG	3 cut(s)	42, 856, 1570
BseSI	GKGCMC	1 cut(s)	1254
BseXI	GCAGC	5 cut(s)	49, 114, 746, 1110, 1542
BseYI	CCCAGC	2 cut(s)	119, 1374
BsgI	GTGCAG	1 cut(s)	1493
Bsh1236I	CGCG	1 cut(s)	594
BsiHKCI	CYCGRG	2 cut(s)	930, 1253
BsiSI	CCGG	3 cut(s)	214, 640, 1254
BslI	CCNNNNNNNGG	5 cut(s)	1209, 1374, 1394, 1454, 1565
BsmAI	GTCTC	3 cut(s)	203, 372, 1297
BsmI	GAATGC	1 cut(s)	104
Bso31I	GGTCTC	2 cut(s)	203, 1297
BsoBI	CYCGRG	2 cut(s)	930, 1253
Bsp1286I	GDGCHC	1 cut(s)	1254
Bsp1407I	TGTACA	1 cut(s)	987
Bsp143I	GATC	2 cut(s)	695, 1000
Bsp1720I	GCTNAGC	1 cut(s)	892
Bsp19I	CCATGG	1 cut(s)	1307
Bsp68I	TCGCGA	1 cut(s)	594
BspACI	CCGC	9 cut(s)	279, 411, 447, 770, 1066, 1125, 1128, 1194, 1427
BspCNI	CTCAG	6 cut(s)	62, 474, 498, 535, 905, 1019
BspFNI	CGCG	1 cut(s)	594
BspLI	GGNNCC	3 cut(s)	46, 1402, 1446
BspPI	GGATC	1 cut(s)	703
BspTNI	GGTCTC	2 cut(s)	203, 1297
BsrBI	CCGCTC	1 cut(s)	281
BsrGI	TGTACA	1 cut(s)	987
BsrI	ACTGG	3 cut(s)	42, 856, 1570
BssMI	GATC	2 cut(s)	695, 1000
BssT1I	CCWWGG	2 cut(s)	66, 1307
Bst2UI	CCWGG	6 cut(s)	888, 1187, 1209, 1389, 1449, 1482
Bst4CI	ACNGT	8 cut(s)	305, 557, 664, 715, 907, 1241, 1289, 1525
Bst6I	CTCTTC	2 cut(s)	303, 1000
BstAPI	GCANNNNNTGC	1 cut(s)	683
BstAUI	TGTACA	1 cut(s)	987
BstC8I	GCNNGC	3 cut(s)	958, 1163, 1192
BstDEI	CTNAG	9 cut(s)	49, 337, 404, 461, 485, 522, 892, 979, 1006
BstDSI	CCRYGG	3 cut(s)	454, 1307, 1329
BstFNI	CGCG	1 cut(s)	594
BstH2I	RGCGCY	1 cut(s)	337
BstHHI	GCGC	3 cut(s)	336, 507, 1161
BstKTI	GATC	2 cut(s)	698, 1003
BstMAI	GTCTC	3 cut(s)	203, 372, 1297
BstMBI	GATC	2 cut(s)	695, 1000
BstMWI	GCNNNNNNNGC	9 cut(s)	278, 471, 683, 731, 875, 1032, 1187, 1460, 1508
BstNI	CCWGG	6 cut(s)	888, 1187, 1209, 1389, 1449, 1482
BstNSI	RCATGY	1 cut(s)	89
BstSCI	CCNGG	9 cut(s)	212, 886, 1185, 1207, 1252, 1253, 1387, 1447, 1480
BstSFI	CTRYAG	5 cut(s)	738, 1075, 1240, 1285, 1524
BstSLI	GKGCMC	1 cut(s)	1254
BstUI	CGCG	1 cut(s)	594
BstV1I	GCAGC	5 cut(s)	49, 114, 746, 1110, 1542
BstV2I	GAAGAC	1 cut(s)	1126
BtgI	CCRYGG	3 cut(s)	454, 1307, 1329
BtsI	GCAGTG	2 cut(s)	684, 1481
BtsIMutI	CAGTG	4 cut(s)	57, 684, 1481, 1521
BtuMI	TCGCGA	1 cut(s)	594
Cac8I	GCNNGC	3 cut(s)	958, 1163, 1192
CaiI	CAGNNNCTG	3 cut(s)	56, 962, 1374
CfoI	GCGC	3 cut(s)	336, 507, 1161
Cfr13I	GGNCC	5 cut(s)	45, 959, 1200, 1445, 1462
Cfr9I	CCCGGG	1 cut(s)	1253
CseI	GACGC	1 cut(s)	853
Csp6I	GTAC	4 cut(s)	35, 573, 988, 1519
CviAII	CATG	8 cut(s)	86, 322, 399, 1094, 1308, 1441, 1490, 1551
CviQI	GTAC	4 cut(s)	35, 573, 988, 1519
DdeI	CTNAG	9 cut(s)	49, 337, 404, 461, 485, 522, 892, 979, 1006
DpnI	GATC	2 cut(s)	697, 1002
DpnII	GATC	2 cut(s)	695, 1000
EaeI	YGGCCR	2 cut(s)	219, 1478
Eam1104I	CTCTTC	2 cut(s)	303, 1000
EarI	CTCTTC	2 cut(s)	303, 1000
EciI	GGCGGA	1 cut(s)	1055
Eco130I	CCWWGG	2 cut(s)	66, 1307
Eco147I	AGGCCT	1 cut(s)	1316
Eco31I	GGTCTC	2 cut(s)	203, 1297
Eco32I	GATATC	1 cut(s)	116
Eco47I	GGWCC	1 cut(s)	1200
Eco47III	AGCGCT	1 cut(s)	335
Eco57I	CTGAAG	3 cut(s)	279, 380, 635
Eco88I	CYCGRG	2 cut(s)	930, 1253
EcoO109I	RGGNCCY	3 cut(s)	45, 959, 1445
EcoRII	CCWGG	6 cut(s)	886, 1185, 1207, 1387, 1447, 1480
EcoRV	GATATC	1 cut(s)	116
EcoT14I	CCWWGG	2 cut(s)	66, 1307
ErhI	CCWWGG	2 cut(s)	66, 1307
FaeI	CATG	8 cut(s)	89, 325, 402, 1097, 1311, 1444, 1493, 1554
FalI	AAGNNNNNCTT	4 cut(s)	356, 388, 1208, 1240
FatI	CATG	8 cut(s)	85, 321, 398, 1093, 1307, 1440, 1489, 1550
FauI	CCCGC	3 cut(s)	286, 763, 1187
FbaI	TGATCA	1 cut(s)	1000
FblI	GTMKAC	1 cut(s)	492
Fnu4HI	GCNGC	9 cut(s)	63, 103, 411, 447, 735, 1099, 1125, 1128, 1556
Fsp4HI	GCNGC	9 cut(s)	63, 103, 411, 447, 735, 1099, 1125, 1128, 1556
FspBI	CTAG	4 cut(s)	111, 471, 861, 1323
FspI	TGCGCA	1 cut(s)	1160
GlaI	GCGC	3 cut(s)	335, 506, 1160
GluI	GCNGC	9 cut(s)	63, 103, 411, 447, 735, 1099, 1125, 1128, 1556
GsaI	CCCAGC	2 cut(s)	123, 1378
GsuI	CTGGAG	2 cut(s)	1416, 1476
HaeII	RGCGCY	1 cut(s)	337
HapII	CCGG	3 cut(s)	214, 640, 1254
HgaI	GACGC	1 cut(s)	853
HhaI	GCGC	3 cut(s)	336, 507, 1161
Hin1II	CATG	8 cut(s)	89, 325, 402, 1097, 1311, 1444, 1493, 1554
Hin6I	GCGC	3 cut(s)	334, 505, 1159
HinP1I	GCGC	3 cut(s)	334, 505, 1159
HincII	GTYRAC	3 cut(s)	193, 493, 553
HindII	GTYRAC	3 cut(s)	193, 493, 553
HinfI	GANTC	2 cut(s)	481, 857
HpaII	CCGG	3 cut(s)	214, 640, 1254
HphI	GGTGA	3 cut(s)	275, 895, 1345
Hpy166II	GTNNAC	5 cut(s)	193, 493, 553, 1434, 1521
Hpy188I	TCNGA	9 cut(s)	229, 259, 360, 478, 721, 1000, 1118, 1204, 1261
Hpy188III	TCNNGA	4 cut(s)	593, 932, 1138, 1234
Hpy8I	GTNNAC	5 cut(s)	193, 493, 553, 1434, 1521
Hpy99I	CGWCG	1 cut(s)	869
HpyAV	CCTTC	3 cut(s)	760, 1306, 1569
HpyCH4III	ACNGT	8 cut(s)	305, 557, 664, 715, 907, 1241, 1289, 1525
HpyCH4IV	ACGT	2 cut(s)	431, 815
HpyCH4V	TGCA	7 cut(s)	85, 102, 435, 686, 1146, 1339, 1474
HpyF10VI	GCNNNNNNNGC	9 cut(s)	278, 471, 683, 731, 875, 1032, 1187, 1460, 1508
HpyF3I	CTNAG	9 cut(s)	49, 337, 404, 461, 485, 522, 892, 979, 1006
HpySE526I	ACGT	2 cut(s)	431, 815
Hsp92II	CATG	8 cut(s)	89, 325, 402, 1097, 1311, 1444, 1493, 1554
HspAI	GCGC	3 cut(s)	334, 505, 1159
Ksp22I	TGATCA	1 cut(s)	1000
Kzo9I	GATC	2 cut(s)	695, 1000
LmnI	GCTCC	4 cut(s)	251, 479, 1369, 1499
Lsp1109I	GCAGC	5 cut(s)	49, 114, 746, 1110, 1542
LweI	GCATC	2 cut(s)	873, 1326
MaeI	CTAG	4 cut(s)	111, 471, 861, 1323
MaeII	ACGT	2 cut(s)	431, 815
MaeIII	GTNAC	4 cut(s)	559, 907, 1413, 1529
MalI	GATC	2 cut(s)	697, 1002
MbiI	CCGCTC	1 cut(s)	281
MboI	GATC	2 cut(s)	695, 1000
MboII	GAAGA	7 cut(s)	320, 373, 592, 646, 987, 1105, 1126
MhlI	GDGCHC	1 cut(s)	1254
MlsI	TGGCCA	2 cut(s)	221, 1480
MluCI	AATT	3 cut(s)	239, 588, 1070
MluNI	TGGCCA	2 cut(s)	221, 1480
MmeI	TCCRAC	3 cut(s)	699, 1227, 1368
Mox20I	TGGCCA	2 cut(s)	221, 1480
MroXI	GAANNNNTTC	1 cut(s)	835
MscI	TGGCCA	2 cut(s)	221, 1480
MseI	TTAA	4 cut(s)	90, 314, 792, 1275
MslI	CAYNNNNRTG	1 cut(s)	1439
Msp20I	TGGCCA	2 cut(s)	221, 1480
MspI	CCGG	3 cut(s)	214, 640, 1254
MspR9I	CCNGG	9 cut(s)	214, 888, 1187, 1209, 1254, 1255, 1389, 1449, 1482
Mva1269I	GAATGC	1 cut(s)	104
MvaI	CCWGG	6 cut(s)	888, 1187, 1209, 1389, 1449, 1482
MvnI	CGCG	1 cut(s)	594
MwoI	GCNNNNNNNGC	9 cut(s)	278, 471, 683, 731, 875, 1032, 1187, 1460, 1508
NciI	CCSGG	3 cut(s)	214, 1254, 1255
NcoI	CCATGG	1 cut(s)	1307
NdeII	GATC	2 cut(s)	695, 1000
NlaIII	CATG	8 cut(s)	89, 325, 402, 1097, 1311, 1444, 1493, 1554
NlaIV	GGNNCC	3 cut(s)	46, 1402, 1446
NmuCI	GTSAC	1 cut(s)	1413
NruI	TCGCGA	1 cut(s)	594
NsbI	TGCGCA	1 cut(s)	1160
NspI	RCATGY	1 cut(s)	89
PaeR7I	CTCGAG	1 cut(s)	930
PasI	CCCWGGG	1 cut(s)	1208
PceI	AGGCCT	1 cut(s)	1316
PcsI	WCGNNNNNNNCGW	2 cut(s)	1128, 1326
PctI	GAATGC	1 cut(s)	104
PdmI	GAANNNNTTC	1 cut(s)	835
PfeI	GAWTC	2 cut(s)	481, 857
PflFI	GACNNNGTC	2 cut(s)	322, 715
PflMI	CCANNNNNTGG	1 cut(s)	1374
PkrI	GCNGC	9 cut(s)	64, 104, 412, 448, 736, 1100, 1126, 1129, 1557
PsiI	TTATAA	1 cut(s)	780
Psp6I	CCWGG	6 cut(s)	886, 1185, 1207, 1387, 1447, 1480
PspFI	CCCAGC	2 cut(s)	119, 1374
PspGI	CCWGG	6 cut(s)	886, 1185, 1207, 1387, 1447, 1480
PspN4I	GGNNCC	3 cut(s)	46, 1402, 1446
PspPI	GGNCC	5 cut(s)	45, 959, 1200, 1445, 1462
PstNI	CAGNNNCTG	3 cut(s)	56, 962, 1374
PsyI	GACNNNGTC	2 cut(s)	322, 715
RruI	TCGCGA	1 cut(s)	594
RsaI	GTAC	4 cut(s)	36, 574, 989, 1520
RsaNI	GTAC	4 cut(s)	35, 573, 988, 1519
RseI	CAYNNNNRTG	1 cut(s)	1439
SalI	GTCGAC	1 cut(s)	491
SaqAI	TTAA	4 cut(s)	90, 314, 792, 1275
SatI	GCNGC	9 cut(s)	63, 103, 411, 447, 735, 1099, 1125, 1128, 1556
Sau3AI	GATC	2 cut(s)	695, 1000
Sau96I	GGNCC	5 cut(s)	45, 959, 1200, 1445, 1462
ScrFI	CCNGG	9 cut(s)	214, 888, 1187, 1209, 1254, 1255, 1389, 1449, 1482
SduI	GDGCHC	1 cut(s)	1254
SfaNI	GCATC	2 cut(s)	873, 1326
SfcI	CTRYAG	5 cut(s)	738, 1075, 1240, 1285, 1524
Sfr274I	CTCGAG	1 cut(s)	930
SinI	GGWCC	1 cut(s)	1200
SlaI	CTCGAG	1 cut(s)	930
SmaI	CCCGGG	1 cut(s)	1255
SmiMI	CAYNNNNRTG	1 cut(s)	1439
SmlI	CTYRAG	2 cut(s)	930, 1409
SmoI	CTYRAG	2 cut(s)	930, 1409
Sse9I	AATT	3 cut(s)	239, 588, 1070
SseBI	AGGCCT	1 cut(s)	1316
SsiI	CCGC	9 cut(s)	279, 411, 447, 770, 1066, 1125, 1128, 1194, 1427
SspMI	CTAG	4 cut(s)	111, 471, 861, 1323
StuI	AGGCCT	1 cut(s)	1316
StyD4I	CCNGG	9 cut(s)	212, 886, 1185, 1207, 1252, 1253, 1387, 1447, 1480
StyI	CCWWGG	2 cut(s)	66, 1307
TaaI	ACNGT	8 cut(s)	305, 557, 664, 715, 907, 1241, 1289, 1525
TaiI	ACGT	2 cut(s)	434, 818
TaqI	TCGA	7 cut(s)	492, 839, 881, 931, 1104, 1380, 1562
TasI	AATT	3 cut(s)	239, 588, 1070
TatI	WGTACW	2 cut(s)	987, 1518
TauI	GCSGC	4 cut(s)	413, 449, 1127, 1130
TfiI	GAWTC	2 cut(s)	481, 857
Tru1I	TTAA	4 cut(s)	90, 314, 792, 1275
Tru9I	TTAA	4 cut(s)	90, 314, 792, 1275
TscAI	CASTG	4 cut(s)	57, 684, 1481, 1528
TseFI	GTSAC	1 cut(s)	1413
TseI	GCWGC	5 cut(s)	62, 102, 734, 1098, 1555
Tsp45I	GTSAC	1 cut(s)	1413
TspDTI	ATGAA	5 cut(s)	913, 1082, 1099, 1478, 1539
TspGWI	ACGGA	2 cut(s)	254, 1219
TspMI	CCCGGG	1 cut(s)	1253
TspRI	CASTG	4 cut(s)	57, 684, 1481, 1528
Tth111I	GACNNNGTC	2 cut(s)	322, 715
Van91I	CCANNNNNTGG	1 cut(s)	1374
VpaK11BI	GGWCC	1 cut(s)	1200
XceI	RCATGY	1 cut(s)	89
XhoI	CTCGAG	1 cut(s)	930
XmaI	CCCGGG	1 cut(s)	1253
XmiI	GTMKAC	1 cut(s)	492
XmnI	GAANNNNTTC	1 cut(s)	835
XspI	CTAG	4 cut(s)	111, 471, 861, 1323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.