FvH4_1g09716

1-deoxy-D-xylulose 5-phosphate reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008471
Physical Location & Seq
Forward (+)
5287305 .. 5289061
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09716.t1

Sequence Viewer

Length: 489 bp
ATGAGATCGATCTATCTCCCTCTCCTTGATCTGATCGGCTTGCTTAAGAGCGGCCGTTTTGATCTCGTCGGCTTTCTTGGAGGGCTCGGCGGCGAACTGGTGATGATATTGGACATAGTTCCGGATAAGTTCAGAGTTGTGGCCCTAGCAGCTGGCTCAAATGTGAAGAGGTTCAAACCCAAACTAGTTGCTGTTAGAAATGAATCATTAGTTGATGAGCTTCAAGAGGCTCTATCTGGTTTAGAAGACAAGCCTGAGATCATTCCTGGGGAGCAAGATGCAGTCACAGTAGTTACAGGAATAGTTGGTTGTGCAGGACTGAGGCCTATAGTGGCTGCAATAGAAGCTGGGAAAGACATCGCTTTAGCCAATAAGGAGACTCTGATTGCTGGAGGCCCATTTGTTCTTCCTCTTGCGCACAAGCATAATGTGAAAATTCTTCCTGCTGATTCAGAACATTCTGCAATTTTTCAGGGATTGGCCTGTTGA

Protein Analysis

163

Amino Acids

17.07

Weight (kDa)

5.6

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_reductoisom PF02670 24 - 132 3.6e-22 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 417
AccBSI CCGCTC 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 2 cut(s) 51, 90
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 1 cut(s) 435
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 2 cut(s) 175, 224
AhlI ACTAGT 1 cut(s) 184
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 3 cut(s) 152, 220, 347
AluI AGCT 3 cut(s) 152, 220, 347
Alw26I GTCTC 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 5 cut(s) 52, 141, 323, 394, 480
ApeKI GCWGC 2 cut(s) 149, 335
ApoI RAATTY 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 2 cut(s) 142, 395
AsuHPI GGTGA 1 cut(s) 112
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 161, 322
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 371
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 2 cut(s) 146, 185
BfmI CTRYAG 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 4 cut(s) 52, 91, 150, 336
BlsI GCNGC 4 cut(s) 53, 92, 151, 337
Bme1390I CCNGG 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 142, 395
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 1 cut(s) 267
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 266
BseMII CTCAG 2 cut(s) 246, 311
BseNI ACTGG 1 cut(s) 102
BseX3I CGGCCG 1 cut(s) 52
BseXI GCAGC 2 cut(s) 161, 322
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 5 cut(s) 54, 143, 325, 396, 482
BshVI ATCGAT 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 55
BsiSI CCGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 5 cut(s) 54, 143, 325, 396, 482
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 6 cut(s) 5, 9, 28, 33, 61, 258
BspACI CCGC 2 cut(s) 51, 90
BspANI GGCC 5 cut(s) 54, 143, 325, 396, 482
BspCNI CTCAG 2 cut(s) 247, 312
BspDI ATCGAT 1 cut(s) 8
BspEI TCCGGA 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 51
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 266
BssMI GATC 6 cut(s) 5, 9, 28, 33, 61, 258
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 41, 154
BstDEI CTNAG 2 cut(s) 255, 320
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 6 cut(s) 8, 12, 31, 36, 64, 261
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 6 cut(s) 5, 9, 28, 33, 61, 258
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 2 cut(s) 149, 344
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 161, 322
BstV2I GAAGAC 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 5 cut(s) 54, 143, 325, 396, 482
BsuTUI ATCGAT 1 cut(s) 8
BtgZI GCGATG 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 41, 154
CfoI GCGC 1 cut(s) 418
Cfr13I GGNCC 2 cut(s) 142, 395
ClaI ATCGAT 1 cut(s) 8
DdeI CTNAG 2 cut(s) 255, 320
DpnI GATC 6 cut(s) 7, 11, 30, 35, 63, 260
DpnII GATC 6 cut(s) 5, 9, 28, 33, 61, 258
EaeI YGGCCR 1 cut(s) 52
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 52
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 87
FaiI YATR 3 cut(s) 116, 329, 426
Fnu4HI GCNGC 4 cut(s) 52, 91, 150, 336
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 4 cut(s) 52, 91, 150, 336
FspBI CTAG 2 cut(s) 146, 185
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 1 cut(s) 417
GluI GCNGC 4 cut(s) 52, 91, 150, 336
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 5 cut(s) 54, 143, 325, 396, 482
HapII CCGG 1 cut(s) 122
HhaI GCGC 1 cut(s) 418
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HinfI GANTC 3 cut(s) 203, 379, 449
HpaII CCGG 1 cut(s) 122
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 4 cut(s) 33, 134, 384, 454
Hpy188III TCNNGA 2 cut(s) 122, 224
Hpy99I CGWCG 1 cut(s) 71
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 281, 314, 338, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 344
HpyF3I CTNAG 2 cut(s) 255, 320
HspAI GCGC 1 cut(s) 416
Kpn2I TCCGGA 1 cut(s) 121
Kzo9I GATC 6 cut(s) 5, 9, 28, 33, 61, 258
LmnI GCTCC 1 cut(s) 271
Lsp1109I GCAGC 2 cut(s) 161, 322
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 146, 185
MaeIII GTNAC 2 cut(s) 283, 292
MalI GATC 6 cut(s) 7, 11, 30, 35, 63, 260
MbiI CCGCTC 1 cut(s) 51
MboI GATC 6 cut(s) 5, 9, 28, 33, 61, 258
MboII GAAGA 4 cut(s) 178, 257, 398, 431
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 2 cut(s) 435, 465
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 7 cut(s) 30, 74, 162, 220, 315, 386, 420
MroI TCCGGA 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 149, 344
NdeII GATC 6 cut(s) 5, 9, 28, 33, 61, 258
NmeAIII GCCGAG 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 283
NsbI TGCGCA 1 cut(s) 417
PceI AGGCCT 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 2 cut(s) 203, 449
PkrI GCNGC 4 cut(s) 53, 92, 151, 337
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 265
PspPI GGNCC 2 cut(s) 142, 395
PvuII CAGCTG 1 cut(s) 152
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 4 cut(s) 52, 91, 150, 336
Sau3AI GATC 6 cut(s) 5, 9, 28, 33, 61, 258
Sau96I GGNCC 2 cut(s) 142, 395
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 87
SetI ASST 4 cut(s) 154, 173, 222, 349
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 2 cut(s) 435, 465
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 2 cut(s) 51, 90
SspMI CTAG 2 cut(s) 146, 185
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 8
TasI AATT 2 cut(s) 435, 465
TauI GCSGC 2 cut(s) 54, 93
TfiI GAWTC 2 cut(s) 203, 449
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 2 cut(s) 149, 335
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 216
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 146, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.