FvH4_1g09870

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010179
Physical Location & Seq
Forward (+)
5367289 .. 5370329
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09870.t2

Sequence Viewer

Length: 699 bp
ATGGCAGAGTTGTGCGTTTCTTCTCAGTCCTCCATTCTCCTCCACCGATCCCAAACCGGAATTCTCCCCAACCGCCTAAGCCCCCGAACCTCCACTTCTCTTTCTCTAAGCGGCGCCGGAGCTCCGCGTCTGCGATTCGGGCCTTCCTTTAATCAAGCTCGCAGCTTGAAGCTTGGAGTGGTCTCCGATCCTACAGCTCACAGCTTATTCACCGTTGATTCCGGGACCAAGGGTATCGTGATCGAGCCCGATATCGGCGGCGGCGACGGAGGCGAATTTGGCGGGAGAGGCGGCGGCGGAGGTGGTGGAGGAGGAGGTGATAACAGTAGCGGTAGAGGCGAGGAGGGAGGTGAGGATAGCAGCGAGAAAAAAGGGATGTCGATGTCTCAGAAGCTAACCCTAGCTTATGCTGTGCTTGTTGGAGTGGGTGGTGTTATGGGCTTTCTGAAGAGTGGCAGCAACAAGTCACTGTTGGCAGGAGGAATATCAGCTTCGTTACTCTTTCTTGTTTACACTGAGCTTCCTAAAAGAACAGTTTTTGCATCATCCCTTGGGCTTGGGCTGTCTGCTGCCCTTCTGGTTGTGATGGGCTCTCGTTTCAAGAGGTCAGGGAAGATATTTCCAGCCGGTGTTGTATCTCTTGTTTCGCTCATAATGACTGGTGGCTACTTTCATGGAATTCTACGTGGCATGCACTGA

Protein Analysis

233

Amino Acids

23.44

Weight (kDa)

9.93

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 133 - 223 6.6e-20 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0010179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26240
fragaria_vesca FvH4_1g06370 FvH4_1g06370 FvH4_1g06370 FvH4_1g09870 FvH4_1g09870
malus_domestica MD02G1106000.v1.1 MD15G1225900.v1.1
prunus_persica Prupe.7G189100_v2.0.a1
pyrus_communis pycom02g08390 pycom15g20080
rosa_chinensis RchiOBHm_Chr2g0096571
rosa_laevigata RLG00000016650
rosa_multiflora Rmu_sc0004096.1_g000004
rosa_roxburghii Rroxscaffold_2G00145530
rosa_rugosa Rorug02G0058700
rosa_samantha Rh2AG105800 Rh2BG108300 Rh2CG110100 Rh2DG108900
rosa_wichuraiana Rw2G008190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 113
AccII CGCG 1 cut(s) 127
AclWI GGATC 2 cut(s) 42, 182
AcsI RAATTY 3 cut(s) 60, 275, 678
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 169, 601
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 2 cut(s) 187, 390
AlwI GGATC 2 cut(s) 42, 182
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 4 cut(s) 162, 360, 456, 569
ApoI RAATTY 3 cut(s) 60, 275, 678
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 140, 225
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 202, 329, 362
AvaII GGWCC 1 cut(s) 225
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 3 cut(s) 124, 249, 593
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 4 cut(s) 174, 372, 468, 556
BccI CCATC 1 cut(s) 580
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 187, 390
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 9 cut(s) 112, 163, 259, 262, 292, 295, 361, 457, 570
BlsI GCNGC 9 cut(s) 113, 164, 260, 263, 293, 296, 362, 458, 571
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 225
BmgT120I GGNCC 2 cut(s) 140, 225
BmiI GGNNCC 2 cut(s) 115, 226
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 551
Bpu10I CCTNAGC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 228, 550
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 1 cut(s) 664
BseDI CCNNGG 2 cut(s) 228, 550
BseGI GGATG 2 cut(s) 381, 545
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 38, 401, 507
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 4 cut(s) 29, 324, 327, 356
BseXI GCAGC 4 cut(s) 174, 372, 468, 556
Bsh1236I CGCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 4 cut(s) 57, 117, 222, 627
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 187, 390
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 187
Bsp1286I GDGCHC 3 cut(s) 124, 249, 593
Bsp143I GATC 3 cut(s) 47, 187, 240
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 3 cut(s) 37, 400, 508
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 115, 226
BspPI GGATC 2 cut(s) 42, 182
BspT107I GGYRCC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 2 cut(s) 228, 550
BssMI GATC 3 cut(s) 47, 187, 240
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 228, 550
Bst4CI ACNGT 4 cut(s) 214, 326, 471, 535
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 2 cut(s) 160, 692
BstDEI CTNAG 5 cut(s) 24, 77, 107, 387, 516
BstF5I GGATG 2 cut(s) 381, 545
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 50, 190, 243
BstMAI GTCTC 2 cut(s) 187, 390
BstMBI GATC 3 cut(s) 47, 187, 240
BstMWI GCNNNNNNNGC 5 cut(s) 139, 270, 279, 288, 336
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 4 cut(s) 174, 372, 468, 556
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 2 cut(s) 381, 545
BtsIMutI CAGTG 3 cut(s) 467, 513, 694
Cac8I GCNNGC 2 cut(s) 160, 692
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 140, 225
CseI GACGC 1 cut(s) 116
CviAII CATG 2 cut(s) 674, 691
DdeI CTNAG 5 cut(s) 24, 77, 107, 387, 516
DinI GGCGCC 1 cut(s) 115
DpnI GATC 3 cut(s) 49, 189, 242
DpnII GATC 3 cut(s) 47, 187, 240
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 228, 550
Eco24I GRGCYC 3 cut(s) 124, 249, 593
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 225
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 122
EcoRI GAATTC 2 cut(s) 60, 678
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 228, 550
EcoT38I GRGCYC 3 cut(s) 124, 249, 593
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 2 cut(s) 228, 550
FaeI CATG 2 cut(s) 677, 694
FaiI YATR 5 cut(s) 408, 437, 653, 675, 692
FaqI GGGAC 1 cut(s) 238
FatI CATG 2 cut(s) 673, 690
FauI CCCGC 1 cut(s) 275
Fnu4HI GCNGC 9 cut(s) 112, 163, 259, 262, 292, 295, 361, 457, 570
FokI GGATG 2 cut(s) 388, 532
FriOI GRGCYC 3 cut(s) 124, 249, 593
Fsp4HI GCNGC 9 cut(s) 112, 163, 259, 262, 292, 295, 361, 457, 570
FspBI CTAG 1 cut(s) 401
GlaI GCGC 1 cut(s) 115
GluI GCNGC 9 cut(s) 112, 163, 259, 262, 292, 295, 361, 457, 570
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 142
HapII CCGG 4 cut(s) 57, 117, 222, 627
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 677, 694
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 135, 218
HpaII CCGG 4 cut(s) 57, 117, 222, 627
HphI GGTGA 3 cut(s) 202, 329, 362
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 3 cut(s) 187, 390, 447
Hpy188III TCNNGA 2 cut(s) 238, 601
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 153, 584
HpyCH4III ACNGT 4 cut(s) 214, 326, 471, 535
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 2 cut(s) 542, 694
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 270, 279, 288, 336
HpyF3I CTNAG 5 cut(s) 24, 77, 107, 387, 516
HpySE526I ACGT 1 cut(s) 685
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 677, 694
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
Kzo9I GATC 3 cut(s) 47, 187, 240
LmnI GCTCC 2 cut(s) 119, 127
LpnPI CCDG 9 cut(s) 70, 130, 235, 462, 563, 594, 636, 640, 645
Lsp1109I GCAGC 4 cut(s) 174, 372, 468, 556
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 2 cut(s) 465, 495
MalI GATC 3 cut(s) 49, 189, 242
MboI GATC 3 cut(s) 47, 187, 240
MboII GAAGA 3 cut(s) 12, 460, 625
MhlI GDGCHC 3 cut(s) 124, 249, 593
MluCI AATT 3 cut(s) 60, 275, 678
Mly113I GGCGCC 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 400
MseI TTAA 1 cut(s) 150
MspI CCGG 4 cut(s) 57, 117, 222, 627
MspR9I CCNGG 1 cut(s) 223
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 5 cut(s) 139, 270, 279, 288, 336
NarI GGCGCC 1 cut(s) 114
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 47, 187, 240
NlaIII CATG 2 cut(s) 677, 694
NlaIV GGNNCC 2 cut(s) 115, 226
NmuCI GTSAC 1 cut(s) 465
NspI RCATGY 1 cut(s) 694
PaeI GCATGC 1 cut(s) 694
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 135, 218
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 9 cut(s) 113, 164, 260, 263, 293, 296, 362, 458, 571
PluTI GGCGCC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 115, 226
PspPI GGNCC 2 cut(s) 140, 225
SacI GAGCTC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 9 cut(s) 112, 163, 259, 262, 292, 295, 361, 457, 570
Sau3AI GATC 3 cut(s) 47, 187, 240
Sau96I GGNCC 2 cut(s) 140, 225
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 3 cut(s) 124, 249, 593
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 192
SfoI GGCGCC 1 cut(s) 115
SinI GGWCC 1 cut(s) 225
SphI GCATGC 1 cut(s) 694
Sse9I AATT 3 cut(s) 60, 275, 678
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 1 cut(s) 401
SstI GAGCTC 1 cut(s) 124
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 2 cut(s) 228, 550
TaaI ACNGT 4 cut(s) 214, 326, 471, 535
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 243, 380
TasI AATT 3 cut(s) 60, 275, 678
TauI GCSGC 5 cut(s) 114, 261, 264, 294, 297
TfiI GAWTC 2 cut(s) 135, 218
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 2 cut(s) 474, 520
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 4 cut(s) 162, 360, 456, 569
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 1 cut(s) 662
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 2 cut(s) 474, 520
VpaK11BI GGWCC 1 cut(s) 225
XapI RAATTY 3 cut(s) 60, 275, 678
XceI RCATGY 1 cut(s) 694
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.