MD02G1106000.v1.1

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010179
Physical Location & Seq
Reverse (-)
8594441 .. 8596261
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1106000.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCAGATTTGTGCGTTTCCCAGTCTTCCCTTTTCCTCCTAAGCCCCAAAATCGGAGCTTTCCACAACACCGCCATTTCTCTTCCCCTTGCCTGCGGCGGAGCTCGGCGCCTGCAACACAACAACAATCTCACCTTTCACGGATTCAGAGCTTCTCCCGGTTTGAGGCTTGGAGTGGTTTCCGACACTAAAACTTCCACCGTATTCACTGTTGATTCGGGAACCAAGGATATCGGTATCGAACCGGTTGCCGGCGGCGGAGACGGTGGGGGTGAGTTTGGCGGTAGGGGAGGCGGCGGCGGCGGCGGAGGCGGTGGAGGTGATAATAGTGAGGGTAAAGGGGAGGGTGAGGAGGGACCTGAAGAGAGCAAGAAGAAAGGGATGTCGATGTCTCAGAAACTAACCCTAGGATATGCTGCTCTTGTTGGAGTGGGTGGCCTTATGGGCTTTTTGAAGAGTGGCAGCCAGAAGTCACTGTTGGCAGGGGGAATATCATCGGCCTTACTGTTTTATGTTTCTACTGAGCTTCCCGTAAGACCAGTTTTTGCATCATCTATTGGGCTTGGGTTGTCTGCTGCCCTCCTGGCTGTGATGGGATCTCGTTTCAAGAAGTCAGGGAAGATCTTTCCAGCCGGCGTTGTCTCTCTTGTGTCGCTTGTAATGACCGGTGGCTACTTACATGGAATTCTACGCAGTGCGCACTGA

Protein Analysis

235

Amino Acids

23.4

Weight (kDa)

9.45

Isoelectric Point (pI)

54.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 135 - 225 1.1e-19 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26240
fragaria_vesca FvH4_1g06370 FvH4_1g06370 FvH4_1g06370 FvH4_1g09870 FvH4_1g09870
malus_domestica MD02G1106000.v1.1 MD15G1225900.v1.1
prunus_persica Prupe.7G189100_v2.0.a1
pyrus_communis pycom02g08390 pycom15g20080
rosa_chinensis RchiOBHm_Chr2g0096571
rosa_laevigata RLG00000016650
rosa_multiflora Rmu_sc0004096.1_g000004
rosa_roxburghii Rroxscaffold_2G00145530
rosa_rugosa Rorug02G0058700
rosa_samantha Rh2AG105800 Rh2BG108300 Rh2CG110100 Rh2DG108900
rosa_wichuraiana Rw2G008190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 108
AclWI GGATC 1 cut(s) 604
AcsI RAATTY 1 cut(s) 684
AcuI CTGAAG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 53, 165, 251
AgeI ACCGGT 2 cut(s) 244, 665
AgsI TTSAA 2 cut(s) 454, 607
AjnI CCWGG 1 cut(s) 582
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 4 cut(s) 59, 104, 152, 526
AluI AGCT 4 cut(s) 59, 104, 152, 526
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 3 cut(s) 255, 396, 646
AlwI GGATC 1 cut(s) 604
AoxI GGCC 2 cut(s) 436, 498
ApeKI GCWGC 3 cut(s) 416, 462, 575
ApoI RAATTY 1 cut(s) 684
AsiGI ACCGGT 2 cut(s) 244, 665
AspA2I CCTAGG 1 cut(s) 406
AspLEI GCGC 2 cut(s) 111, 700
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 4 cut(s) 124, 284, 332, 359
AvaII GGWCC 1 cut(s) 356
AvrII CCTAGG 1 cut(s) 406
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 3 cut(s) 403, 474, 562
BccI CCATC 1 cut(s) 586
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 584
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 255, 396, 646
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 112
BglI GCCNNNNNGGC 2 cut(s) 444, 584
BglII AGATCT 1 cut(s) 621
BisI GCNGC 9 cut(s) 97, 256, 295, 298, 301, 304, 417, 463, 576
BlnI CCTAGG 1 cut(s) 406
BlsI GCNGC 9 cut(s) 98, 257, 296, 299, 302, 305, 418, 464, 577
Bme1390I CCNGG 2 cut(s) 159, 584
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 3 cut(s) 110, 223, 357
BmrFI CCNGG 2 cut(s) 159, 584
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 225, 406
BsaWI WCCGGW 2 cut(s) 244, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 165, 251
Bse118I RCCGGY 4 cut(s) 244, 251, 632, 665
Bse1I ACTGG 2 cut(s) 22, 539
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 225, 406
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 53, 165, 251
BseMII CTCAG 2 cut(s) 407, 513
BseNI ACTGG 2 cut(s) 22, 539
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 3 cut(s) 403, 474, 562
BshFI GGCC 2 cut(s) 438, 500
BshNI GGYRCC 1 cut(s) 108
BshTI ACCGGT 2 cut(s) 244, 665
BsiHKAI GWGCWC 1 cut(s) 106
BsiSI CCGG 5 cut(s) 159, 245, 252, 633, 666
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 53, 165, 251
BsmAI GTCTC 3 cut(s) 255, 396, 646
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 2 cut(s) 438, 500
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 2 cut(s) 596, 621
BspANI GGCC 2 cut(s) 438, 500
BspCNI CTCAG 2 cut(s) 406, 514
BspLI GGNNCC 3 cut(s) 110, 223, 357
BspPI GGATC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 108
BsrFI RCCGGY 4 cut(s) 244, 251, 632, 665
BsrI ACTGG 2 cut(s) 22, 539
BssAI RCCGGY 4 cut(s) 244, 251, 632, 665
BssECI CCNNGG 2 cut(s) 225, 406
BssMI GATC 2 cut(s) 596, 621
BssNI GRCGYC 1 cut(s) 109
BssT1I CCWWGG 2 cut(s) 225, 406
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 5 cut(s) 202, 211, 266, 477, 507
Bst6I CTCTTC 3 cut(s) 87, 357, 449
BstACI GRCGYC 1 cut(s) 109
BstC8I GCNNGC 4 cut(s) 94, 113, 253, 634
BstDEI CTNAG 3 cut(s) 41, 393, 522
BstF5I GGATG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 700
BstKTI GATC 2 cut(s) 599, 624
BstMAI GTCTC 3 cut(s) 255, 396, 646
BstMBI GATC 2 cut(s) 596, 621
BstMWI GCNNNNNNNGC 5 cut(s) 300, 303, 309, 444, 584
BstNI CCWGG 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 157, 582
BstV1I GCAGC 3 cut(s) 403, 474, 562
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 2 cut(s) 596, 621
BstYI RGATCY 2 cut(s) 596, 621
BsuRI GGCC 2 cut(s) 438, 500
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 4 cut(s) 207, 473, 700, 700
Cac8I GCNNGC 4 cut(s) 94, 113, 253, 634
CfoI GCGC 2 cut(s) 111, 700
Cfr10I RCCGGY 4 cut(s) 244, 251, 632, 665
Cfr13I GGNCC 1 cut(s) 356
CspAI ACCGGT 2 cut(s) 244, 665
CviAII CATG 1 cut(s) 680
DdeI CTNAG 3 cut(s) 41, 393, 522
DinI GGCGCC 1 cut(s) 110
DpnI GATC 2 cut(s) 598, 623
DpnII GATC 2 cut(s) 596, 621
Eam1104I CTCTTC 3 cut(s) 87, 357, 449
EarI CTCTTC 3 cut(s) 87, 357, 449
EciI GGCGGA 3 cut(s) 114, 273, 321
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 225, 406
Eco24I GRGCYC 1 cut(s) 106
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 582
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 225, 406
EcoT38I GRGCYC 1 cut(s) 106
EgeI GGCGCC 1 cut(s) 110
EheI GGCGCC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 225, 406
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 1 cut(s) 683
FaiI YATR 4 cut(s) 414, 443, 513, 681
FaqI GGGAC 1 cut(s) 369
FatI CATG 1 cut(s) 679
Fnu4HI GCNGC 9 cut(s) 97, 256, 295, 298, 301, 304, 417, 463, 576
FokI GGATG 1 cut(s) 394
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 9 cut(s) 97, 256, 295, 298, 301, 304, 417, 463, 576
FspAI RTGCGCAY 1 cut(s) 699
FspBI CTAG 1 cut(s) 407
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 2 cut(s) 110, 699
GluI GCNGC 9 cut(s) 97, 256, 295, 298, 301, 304, 417, 463, 576
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 438, 500
HapII CCGG 5 cut(s) 159, 245, 252, 633, 666
HhaI GCGC 2 cut(s) 111, 700
Hin1I GRCGYC 1 cut(s) 109
Hin1II CATG 1 cut(s) 683
Hin6I GCGC 2 cut(s) 109, 698
HinP1I GCGC 2 cut(s) 109, 698
HinfI GANTC 2 cut(s) 144, 215
HpaII CCGG 5 cut(s) 159, 245, 252, 633, 666
HphI GGTGA 4 cut(s) 124, 284, 332, 359
Hpy188I TCNGA 4 cut(s) 56, 149, 184, 396
Hpy188III TCNNGA 2 cut(s) 219, 607
HpyCH4III ACNGT 5 cut(s) 202, 211, 266, 477, 507
HpyCH4V TGCA 2 cut(s) 115, 548
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 303, 309, 444, 584
HpyF3I CTNAG 3 cut(s) 41, 393, 522
Hsp92I GRCGYC 1 cut(s) 109
Hsp92II CATG 1 cut(s) 683
HspAI GCGC 2 cut(s) 109, 698
KasI GGCGCC 1 cut(s) 108
KroI GCCGGC 2 cut(s) 251, 632
KroNI GCCGGC 2 cut(s) 253, 634
Kzo9I GATC 2 cut(s) 596, 621
LmnI GCTCC 2 cut(s) 56, 101
Lsp1109I GCAGC 3 cut(s) 403, 474, 562
LweI GCATC 1 cut(s) 557
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 471
MalI GATC 2 cut(s) 598, 623
MboI GATC 2 cut(s) 596, 621
MboII GAAGA 6 cut(s) 18, 74, 374, 385, 466, 631
MflI RGATCY 2 cut(s) 596, 621
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 1 cut(s) 684
Mly113I GGCGCC 1 cut(s) 109
MmeI TCCRAC 2 cut(s) 207, 406
MroNI GCCGGC 2 cut(s) 251, 632
MspI CCGG 5 cut(s) 159, 245, 252, 633, 666
MspR9I CCNGG 2 cut(s) 159, 584
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 5 cut(s) 300, 303, 309, 444, 584
NaeI GCCGGC 2 cut(s) 253, 634
NarI GGCGCC 1 cut(s) 109
NciI CCSGG 1 cut(s) 159
NdeII GATC 2 cut(s) 596, 621
NgoMIV GCCGGC 2 cut(s) 251, 632
NlaIII CATG 1 cut(s) 683
NlaIV GGNNCC 3 cut(s) 110, 223, 357
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 471
NsbI TGCGCA 1 cut(s) 699
PdiI GCCGGC 2 cut(s) 253, 634
PfeI GAWTC 2 cut(s) 144, 215
PinAI ACCGGT 2 cut(s) 244, 665
PkrI GCNGC 9 cut(s) 98, 257, 296, 299, 302, 305, 418, 464, 577
PluTI GGCGCC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 3 cut(s) 110, 223, 357
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PsuI RGATCY 2 cut(s) 596, 621
SacI GAGCTC 1 cut(s) 106
SatI GCNGC 9 cut(s) 97, 256, 295, 298, 301, 304, 417, 463, 576
Sau3AI GATC 2 cut(s) 596, 621
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 2 cut(s) 159, 584
SduI GDGCHC 1 cut(s) 106
SetI ASST 7 cut(s) 61, 106, 137, 154, 322, 361, 528
SfaNI GCATC 1 cut(s) 557
SfoI GGCGCC 1 cut(s) 110
SinI GGWCC 1 cut(s) 356
Sse9I AATT 1 cut(s) 684
SspDI GGCGCC 1 cut(s) 108
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 2 cut(s) 157, 582
StyI CCWWGG 2 cut(s) 225, 406
TaaI ACNGT 5 cut(s) 202, 211, 266, 477, 507
TaqI TCGA 2 cut(s) 240, 386
TasI AATT 1 cut(s) 684
TauI GCSGC 6 cut(s) 99, 258, 297, 300, 303, 306
TfiI GAWTC 2 cut(s) 144, 215
TscAI CASTG 3 cut(s) 214, 480, 700
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 3 cut(s) 416, 462, 575
Tsp45I GTSAC 1 cut(s) 471
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 3 cut(s) 214, 480, 700
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 684
XmaJI CCTAGG 1 cut(s) 406
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.