Rw2G008190

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010179
Physical Location & Seq
Reverse (-)
8109846 .. 8112881
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008190.1

Sequence Viewer

Length: 699 bp
ATGGCAGAGTTAAGCGTTTCTTCTCAGTCCTCCTTTCTCCTCCTCCGACCCAAAACCGGAATTCTCCACAACCCCCGAACCGCCATTTCTCTTTCTCTAAGCGGCGCCGGAGCTCCGTGCCTGCGATTCGCGGCTTCCTCTAATCAAGCTCGTAGCTTGAGGCTCGGGGTGGTCTCCGATCCTACAGCTCCCAGCTTATTCACCGTTGATTCGGGCACTAAGGGTATTGTGATCGAGCCCGAGATCGGCGGCGGCGGCGGCGGCGACGGAGGCGATTCTGGCGGGAGAGGCGGCGGCGGAGGAGGAGGAGGAGGAGGGGATCATAGTGACGGTAGAGGCGAGGAGGGAGCTGACGACAGTAGCAAGAAGAAAGGGATGTCGATGTCTCAGAAACTAACCCTAGCTTATGCTCTGCTTGTTGGAGTGGGTGGCGTTATGGGCTTTTTGAAGAGCGGCAGCAACAAGTCACTGTTGGCAGGAGGAATATCAGCATCCTTACTCTATCTTGTTTATACTGAGCTTCCTAAAAGAACAGTTTTTGCATCATCTCTTGGGCTTGGGCTGTCTGCTGCCCTTCTGGTTGTGATGGGCTCTCGTTTCAAGAGGTCAGGGAAGATATTTCCAGCCGGCGTTGTATCTCTCGTGTCACTCATAATGACTGGTGGCTACTTTCATGGAATTCTACGTGGCATGCACTGA

Protein Analysis

232

Amino Acids

23.17

Weight (kDa)

9.83

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 133 - 223 9.2e-20 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0010179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26240
fragaria_vesca FvH4_1g06370 FvH4_1g06370 FvH4_1g06370 FvH4_1g09870 FvH4_1g09870
malus_domestica MD02G1106000.v1.1 MD15G1225900.v1.1
prunus_persica Prupe.7G189100_v2.0.a1
pyrus_communis pycom02g08390 pycom15g20080
rosa_chinensis RchiOBHm_Chr2g0096571
rosa_laevigata RLG00000016650
rosa_multiflora Rmu_sc0004096.1_g000004
rosa_roxburghii Rroxscaffold_2G00145530
rosa_rugosa Rorug02G0058700
rosa_samantha Rh2AG105800 Rh2BG108300 Rh2CG110100 Rh2DG108900
rosa_wichuraiana Rw2G008190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 453
AccII CGCG 1 cut(s) 131
AclWI GGATC 2 cut(s) 173, 327
AcsI RAATTY 2 cut(s) 60, 678
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 56, 245
AgsI TTSAA 2 cut(s) 448, 601
AluBI AGCT 8 cut(s) 113, 149, 156, 188, 195, 350, 404, 520
AluI AGCT 8 cut(s) 113, 149, 156, 188, 195, 350, 404, 520
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 178, 390
AlwI GGATC 2 cut(s) 173, 327
Ama87I CYCGRG 2 cut(s) 164, 239
ApeKI GCWGC 2 cut(s) 456, 569
ApoI RAATTY 2 cut(s) 60, 678
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 2 cut(s) 164, 239
BaeGI GKGCMC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 3 cut(s) 115, 240, 593
BauI CACGAG 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 468, 556
BccI CCATC 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 178, 390
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 108
BmeT110I CYCGRG 2 cut(s) 164, 239
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 2 cut(s) 500, 551
BpuEI CTTGAG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 245
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 1 cut(s) 664
BseGI GGATG 2 cut(s) 381, 491
BseLI CCNNNNNNNGG 2 cut(s) 56, 245
BseMII CTCAG 3 cut(s) 38, 401, 507
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 8 cut(s) 29, 32, 315, 318, 321, 324, 327, 356
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 2 cut(s) 468, 556
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 2 cut(s) 164, 239
BsiSI CCGG 3 cut(s) 57, 108, 627
BslI CCNNNNNNNGG 2 cut(s) 56, 245
BsmAI GTCTC 2 cut(s) 178, 390
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 2 cut(s) 164, 239
Bsp1286I GDGCHC 4 cut(s) 115, 218, 240, 593
Bsp143I GATC 4 cut(s) 178, 231, 243, 319
BspCNI CTCAG 3 cut(s) 37, 400, 508
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 106
BspPI GGATC 2 cut(s) 173, 327
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 178
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 626
BssMI GATC 4 cut(s) 178, 231, 243, 319
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 641
Bst2BI CACGAG 1 cut(s) 641
Bst4CI ACNGT 5 cut(s) 205, 332, 359, 471, 535
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 122, 628, 692
BstDEI CTNAG 5 cut(s) 24, 98, 219, 387, 516
BstF5I GGATG 2 cut(s) 381, 491
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 4 cut(s) 181, 234, 246, 322
BstMAI GTCTC 2 cut(s) 178, 390
BstMBI GATC 4 cut(s) 178, 231, 243, 319
BstMWI GCNNNNNNNGC 7 cut(s) 255, 258, 261, 270, 279, 288, 438
BstNSI RCATGY 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 468, 556
BtsCI GGATG 2 cut(s) 381, 491
BtsIMutI CAGTG 2 cut(s) 467, 694
Cac8I GCNNGC 3 cut(s) 122, 628, 692
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 626
CviAII CATG 2 cut(s) 674, 691
DdeI CTNAG 5 cut(s) 24, 98, 219, 387, 516
DinI GGCGCC 1 cut(s) 106
DpnI GATC 4 cut(s) 180, 233, 245, 321
DpnII GATC 4 cut(s) 178, 231, 243, 319
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 113
Eco24I GRGCYC 3 cut(s) 115, 240, 593
Eco31I GGTCTC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 113
Eco88I CYCGRG 2 cut(s) 164, 239
EcoICRI GAGCTC 1 cut(s) 113
EcoRI GAATTC 2 cut(s) 60, 678
EcoT38I GRGCYC 3 cut(s) 115, 240, 593
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 2 cut(s) 677, 694
FaiI YATR 7 cut(s) 324, 408, 437, 513, 653, 675, 692
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 2 cut(s) 673, 690
FauI CCCGC 1 cut(s) 275
FokI GGATG 2 cut(s) 388, 478
FriOI GRGCYC 3 cut(s) 115, 240, 593
FspBI CTAG 1 cut(s) 401
GlaI GCGC 1 cut(s) 106
GsaI CCCAGC 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 108
HapII CCGG 3 cut(s) 57, 108, 627
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 677, 694
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 126, 209, 275
HpaII CCGG 3 cut(s) 57, 108, 627
HphI GGTGA 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 47, 178, 390
Hpy188III TCNNGA 1 cut(s) 601
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 5 cut(s) 205, 332, 359, 471, 535
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 2 cut(s) 542, 694
HpyF10VI GCNNNNNNNGC 7 cut(s) 255, 258, 261, 270, 279, 288, 438
HpyF3I CTNAG 5 cut(s) 24, 98, 219, 387, 516
HpySE526I ACGT 1 cut(s) 685
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 677, 694
HspAI GCGC 1 cut(s) 105
KasI GGCGCC 1 cut(s) 104
KroI GCCGGC 1 cut(s) 626
KroNI GCCGGC 1 cut(s) 628
Kzo9I GATC 4 cut(s) 178, 231, 243, 319
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 4 cut(s) 110, 118, 193, 347
Lsp1109I GCAGC 2 cut(s) 468, 556
LweI GCATC 2 cut(s) 500, 551
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 3 cut(s) 326, 465, 645
MalI GATC 4 cut(s) 180, 233, 245, 321
MbiI CCGCTC 1 cut(s) 453
MboI GATC 4 cut(s) 178, 231, 243, 319
MboII GAAGA 4 cut(s) 12, 379, 460, 625
MhlI GDGCHC 4 cut(s) 115, 218, 240, 593
MluCI AATT 2 cut(s) 60, 678
Mly113I GGCGCC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 70, 400
MroNI GCCGGC 1 cut(s) 626
MseI TTAA 1 cut(s) 11
MspI CCGG 3 cut(s) 57, 108, 627
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 255, 258, 261, 270, 279, 288, 438
NaeI GCCGGC 1 cut(s) 628
NarI GGCGCC 1 cut(s) 105
NdeII GATC 4 cut(s) 178, 231, 243, 319
NgoMIV GCCGGC 1 cut(s) 626
NlaIII CATG 2 cut(s) 677, 694
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 3 cut(s) 326, 465, 645
NspI RCATGY 1 cut(s) 694
PaeI GCATGC 1 cut(s) 694
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdiI GCCGGC 1 cut(s) 628
PfeI GAWTC 3 cut(s) 126, 209, 275
PluTI GGCGCC 1 cut(s) 108
Ppu21I YACGTR 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 106
SacI GAGCTC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 11
Sau3AI GATC 4 cut(s) 178, 231, 243, 319
SduI GDGCHC 4 cut(s) 115, 218, 240, 593
SfaNI GCATC 2 cut(s) 500, 551
SfcI CTRYAG 1 cut(s) 183
SfoI GGCGCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SphI GCATGC 1 cut(s) 694
Sse9I AATT 2 cut(s) 60, 678
SspDI GGCGCC 1 cut(s) 104
SspMI CTAG 1 cut(s) 401
SstI GAGCTC 1 cut(s) 115
TaaI ACNGT 5 cut(s) 205, 332, 359, 471, 535
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 234, 380
TasI AATT 2 cut(s) 60, 678
TfiI GAWTC 3 cut(s) 126, 209, 275
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 1 cut(s) 474
TseFI GTSAC 3 cut(s) 326, 465, 645
TseI GCWGC 2 cut(s) 456, 569
Tsp45I GTSAC 3 cut(s) 326, 465, 645
TspDTI ATGAA 1 cut(s) 662
TspGWI ACGGA 2 cut(s) 105, 282
TspRI CASTG 1 cut(s) 474
XapI RAATTY 2 cut(s) 60, 678
XceI RCATGY 1 cut(s) 694
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.