Rroxscaffold_2G00145530

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010179
Physical Location & Seq
Forward (+)
83425729 .. 83428600
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145530.1

Sequence Viewer

Length: 690 bp
ATGGCAGAGTTAAGCGTTTCTTCTCAGTCCTCCTTTCTCCTCCTCCGACCCAAAACCGGAATTCTCCACAACCCCCGAACCGCCATTTCTCTTTCTCTAAGCGGCGCCGGAGCTCCGCACCTGCGATTCGCGGCTTCCTCCGATCAAGCTCGTAGCTTGAGGCTCGGGGTGGTCTCCGATCCTACAGCTCCCAGCTTATTCACCGTTGATTCGGGCACTAAGGGTATTGTGATCGAGCCCGAGATCGGCGGCGGCGGCGGAGGCGGCGACGGAGGCGATTCTGGCGGGAGAGGCGGCGGAGGAGGAGGGGATCATAGTGACGACAGAGGCGAGGAGGGAGCTGACGATAGTAGCAAGAAGAAAGGGATGTCGATGTCTCAGAAACTAACCCTAGCTTATGCTCTGCTTGTTGGAGTGGGTGGCGTTATGGGCTTTTTGAAGAGCGGCAGCAACAAGTCACTGTTGGCAGGAGGAATATCAGCATCCTTACTTTATCTTGTTTATACTGAGCTTCCTAAAAGAACAGTTTTTGCATCATCTCTTGGGCTTGGGCTGTCTGCTGCCCTTCTGGTTGTGATGGGCTCTCGTTTCAAGAGGTCAGGGAAGATATTTCCAGCCGGCGTTGTATCTCTCGTGTCACTCATAATGACTGGTGGCTACTTTCATGGAATTCTACGTGGCATGCACTGA

Protein Analysis

229

Amino Acids

23.1

Weight (kDa)

9.69

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 130 - 220 8.9e-20 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0010179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26240
fragaria_vesca FvH4_1g06370 FvH4_1g06370 FvH4_1g06370 FvH4_1g09870 FvH4_1g09870
malus_domestica MD02G1106000.v1.1 MD15G1225900.v1.1
prunus_persica Prupe.7G189100_v2.0.a1
pyrus_communis pycom02g08390 pycom15g20080
rosa_chinensis RchiOBHm_Chr2g0096571
rosa_laevigata RLG00000016650
rosa_multiflora Rmu_sc0004096.1_g000004
rosa_roxburghii Rroxscaffold_2G00145530
rosa_rugosa Rorug02G0058700
rosa_samantha Rh2AG105800 Rh2BG108300 Rh2CG110100 Rh2DG108900
rosa_wichuraiana Rw2G008190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 444
AccII CGCG 1 cut(s) 131
AclWI GGATC 2 cut(s) 173, 318
AcsI RAATTY 2 cut(s) 60, 669
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 56, 245
AgsI TTSAA 2 cut(s) 439, 592
AluBI AGCT 8 cut(s) 113, 149, 156, 188, 195, 341, 395, 511
AluI AGCT 8 cut(s) 113, 149, 156, 188, 195, 341, 395, 511
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 178, 381
AlwI GGATC 2 cut(s) 173, 318
Ama87I CYCGRG 2 cut(s) 164, 239
ApeKI GCWGC 2 cut(s) 447, 560
ApoI RAATTY 2 cut(s) 60, 669
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 2 cut(s) 164, 239
BaeGI GKGCMC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 3 cut(s) 115, 240, 584
BauI CACGAG 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 459, 547
BccI CCATC 1 cut(s) 571
BcoDI GTCTC 2 cut(s) 178, 381
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 129
BmeT110I CYCGRG 2 cut(s) 164, 239
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 2 cut(s) 491, 542
BpuEI CTTGAG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 245
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 655
BseGI GGATG 2 cut(s) 372, 482
BseLI CCNNNNNNNGG 2 cut(s) 56, 245
BseMII CTCAG 3 cut(s) 38, 392, 498
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 5 cut(s) 29, 32, 315, 318, 347
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 2 cut(s) 459, 547
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 2 cut(s) 164, 239
BsiSI CCGG 3 cut(s) 57, 108, 618
BslI CCNNNNNNNGG 2 cut(s) 56, 245
BsmAI GTCTC 2 cut(s) 178, 381
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 2 cut(s) 164, 239
Bsp1286I GDGCHC 4 cut(s) 115, 218, 240, 584
Bsp143I GATC 5 cut(s) 142, 178, 231, 243, 310
BspCNI CTCAG 3 cut(s) 37, 391, 499
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 129
BspPI GGATC 2 cut(s) 173, 318
BspQI GCTCTTC 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 178
BsrBI CCGCTC 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 617
BssMI GATC 5 cut(s) 142, 178, 231, 243, 310
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 632
Bst2BI CACGAG 1 cut(s) 632
Bst4CI ACNGT 3 cut(s) 205, 462, 526
Bst6I CTCTTC 1 cut(s) 434
BstACI GRCGYC 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 2 cut(s) 619, 683
BstDEI CTNAG 5 cut(s) 24, 98, 219, 378, 507
BstF5I GGATG 2 cut(s) 372, 482
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 5 cut(s) 145, 181, 234, 246, 313
BstMAI GTCTC 2 cut(s) 178, 381
BstMBI GATC 5 cut(s) 142, 178, 231, 243, 310
BstMWI GCNNNNNNNGC 7 cut(s) 255, 261, 264, 273, 282, 291, 429
BstNSI RCATGY 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 459, 547
BtsCI GGATG 2 cut(s) 372, 482
BtsIMutI CAGTG 2 cut(s) 458, 685
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 619, 683
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 617
CviAII CATG 2 cut(s) 665, 682
DdeI CTNAG 5 cut(s) 24, 98, 219, 378, 507
DinI GGCGCC 1 cut(s) 106
DpnI GATC 5 cut(s) 144, 180, 233, 245, 312
DpnII GATC 5 cut(s) 142, 178, 231, 243, 310
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
EciI GGCGGA 2 cut(s) 273, 312
Ecl136II GAGCTC 1 cut(s) 113
Eco24I GRGCYC 3 cut(s) 115, 240, 584
Eco31I GGTCTC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 113
Eco88I CYCGRG 2 cut(s) 164, 239
EcoICRI GAGCTC 1 cut(s) 113
EcoRI GAATTC 2 cut(s) 60, 669
EcoT38I GRGCYC 3 cut(s) 115, 240, 584
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 2 cut(s) 668, 685
FaiI YATR 7 cut(s) 315, 399, 428, 504, 644, 666, 683
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 2 cut(s) 664, 681
FauI CCCGC 1 cut(s) 278
FokI GGATG 2 cut(s) 379, 469
FriOI GRGCYC 3 cut(s) 115, 240, 584
FspBI CTAG 1 cut(s) 392
GlaI GCGC 1 cut(s) 106
GsaI CCCAGC 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 108
HapII CCGG 3 cut(s) 57, 108, 618
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 668, 685
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 126, 209, 278
HpaII CCGG 3 cut(s) 57, 108, 618
HphI GGTGA 1 cut(s) 193
Hpy188I TCNGA 4 cut(s) 47, 142, 178, 381
Hpy188III TCNNGA 1 cut(s) 592
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 3 cut(s) 205, 462, 526
HpyCH4IV ACGT 1 cut(s) 676
HpyCH4V TGCA 2 cut(s) 533, 685
HpyF10VI GCNNNNNNNGC 7 cut(s) 255, 261, 264, 273, 282, 291, 429
HpyF3I CTNAG 5 cut(s) 24, 98, 219, 378, 507
HpySE526I ACGT 1 cut(s) 676
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 668, 685
HspAI GCGC 1 cut(s) 105
KasI GGCGCC 1 cut(s) 104
KroI GCCGGC 1 cut(s) 617
KroNI GCCGGC 1 cut(s) 619
Kzo9I GATC 5 cut(s) 142, 178, 231, 243, 310
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 4 cut(s) 110, 118, 193, 338
Lsp1109I GCAGC 2 cut(s) 459, 547
LweI GCATC 2 cut(s) 491, 542
MaeI CTAG 1 cut(s) 392
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 3 cut(s) 317, 456, 636
MalI GATC 5 cut(s) 144, 180, 233, 245, 312
MbiI CCGCTC 1 cut(s) 444
MboI GATC 5 cut(s) 142, 178, 231, 243, 310
MboII GAAGA 4 cut(s) 12, 370, 451, 616
MhlI GDGCHC 4 cut(s) 115, 218, 240, 584
MluCI AATT 2 cut(s) 60, 669
Mly113I GGCGCC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 70, 391
MroNI GCCGGC 1 cut(s) 617
MseI TTAA 1 cut(s) 11
MspI CCGG 3 cut(s) 57, 108, 618
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 255, 261, 264, 273, 282, 291, 429
NaeI GCCGGC 1 cut(s) 619
NarI GGCGCC 1 cut(s) 105
NdeII GATC 5 cut(s) 142, 178, 231, 243, 310
NgoMIV GCCGGC 1 cut(s) 617
NlaIII CATG 2 cut(s) 668, 685
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 3 cut(s) 317, 456, 636
NspI RCATGY 1 cut(s) 685
PaeI GCATGC 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 434
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PdiI GCCGGC 1 cut(s) 619
PfeI GAWTC 3 cut(s) 126, 209, 278
PluTI GGCGCC 1 cut(s) 108
Ppu21I YACGTR 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 106
SacI GAGCTC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 1 cut(s) 11
Sau3AI GATC 5 cut(s) 142, 178, 231, 243, 310
SduI GDGCHC 4 cut(s) 115, 218, 240, 584
SfaNI GCATC 2 cut(s) 491, 542
SfcI CTRYAG 1 cut(s) 183
SfoI GGCGCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SphI GCATGC 1 cut(s) 685
Sse9I AATT 2 cut(s) 60, 669
SspDI GGCGCC 1 cut(s) 104
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 115
TaaI ACNGT 3 cut(s) 205, 462, 526
TaiI ACGT 1 cut(s) 679
TaqI TCGA 2 cut(s) 234, 371
TasI AATT 2 cut(s) 60, 669
TauI GCSGC 8 cut(s) 105, 134, 252, 255, 258, 267, 297, 447
TfiI GAWTC 3 cut(s) 126, 209, 278
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 3 cut(s) 317, 456, 636
TseI GCWGC 2 cut(s) 447, 560
Tsp45I GTSAC 3 cut(s) 317, 456, 636
TspDTI ATGAA 1 cut(s) 653
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 1 cut(s) 465
XapI RAATTY 2 cut(s) 60, 669
XceI RCATGY 1 cut(s) 685
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.