Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009247

Physical Location & Seq

Genomic Coordinates Forward (+)

7553261 .. 7556942

Gene Structure 3682 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g13710.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGCCGACGGACTTGGATAACTCCTCCACAGCTTCAGGGGAGGCTAGTGTTTCTTCCTCCGGTAACCAAACTGCTCCGGCTCCAAAACCCATCACCAAGAAAAAGCGGAACTTGCCCGGAATGCCAGATCCAGATGCAGAGGTGATTGCTCTGTCTCCGAAGACCCTTATGGCAACGAACCGATTTGTATGCGAAATTTGCAACAAAGGGTTTCAGAGAGATCAGAATCTGCAGCTCCATCGGCGAGGTCATAATCTGCCTTGGAAGTTGAGGCAGAGATCAAGCAAAGAGGTCAAGAAGAGAGTCTATGTCTGCCCTGAAGTTTCTTGCGTCCACCATGATCCTTCTAGAGCTTTGGGTGATCTGACTGGGATTAAGAAGCACTTTTGTAGAAAGCACGGTGAGAAGAAGTGGAAATGCGACAAGTGCTCGAAGAAATATGCGGTTCAGTCGGATTGGAAAGCTCATTCTAAGATTTGTGGTACCAGGGAGTATAAATGTGATTGTGGGACTTTGTTTTCAAGGAGGGATAGCTTCATAACACACAGGGCTTTCTGTGATGCTTTGGCTGAGGAAAGTGCTAGAGGTCCTTCTCAGACTCAAAATGTGAAGTCGAATTCAGAATCAGACCCAAAGGTCCAAAGAGCAAATTCACCTCCACCTCCACCACCAGCACAGGCTTCAGTTCCATCCCAACCCTCAAGTTCTTCAGCTCCCTCTCAGCCACCAATTCTGCCTATTCAAAGTTCAGTGCCAGAGTTGCCTGAAAATCCCCCAGAAGATGCCCCTGTTGTGACTCAGCGGAAAGGACACTGTAGCAGCAGCAACAGCTCAAGCAGCAATGGTAGTACAAGCAGCAGTGTATTTGCAAGTTTATTTGCTTCATCGACAACATCTGGAGCAAGTTTGCAACCTCCTCAGCCTCCTGCATTTACAGACTTAATTCGAGCCATGGGGCAACCGGACCTACCAACTGACCTAGCACCATCTGCTTCAATCGAGCCTATCTCTCTTGGCCTCTCTACAAGTCATGCATCCTCAATTTTTGGGACTGCAGGACAGGAGCGTAGGCAGTATGCGCCTCCACCACAACCAGCTATGTCTGCCACGGCACTACTTCAGAAAGCTGCTCAAATGAGCGCAACATCTAATGCATCGTTGCTCCGTGGGTTTGGCATTGTGTCCTCCTCATCTTCTTCCGCAGAGCAAGAGAGCATGCAATGGAATCAGCGGCAAACAGAATCAGATAATGCCTCAATTGCTGCAGGGCTTGGACTTGGTCTTCCTTGTGATGGTTCTGGATGGAAGGAATTAATGATGGGAAGTCCTTCAATGTTTGGTCCTAAGCAAACCACACTTGATCTTTTGGGATTGGGAATGGCTGCCGGTAATAACCCCAATGGTGGCCTATCAGCCCTAATTACATCCATCAGTGGTGGTCTAGATGTGGCTGCTGCAGCTGCATCATTTGGAAGCGGAGAATATAGTGGCAAGGATTTGGCAAGGAGCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0009937 GO:0009966 GO:0010029 GO:0010154 GO:0010431 GO:0010468 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023051 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048316 GO:0048580 GO:0048583 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0097159 GO:0140110 GO:1900140 GO:1901363 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C2H2

Protein Analysis

505

Amino Acids

53.13

Weight (kDa)

8.96

Isoelectric Point (pI)

62.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2H2-2nd_BIRD-IDD	PF22996	99 - 133	1.4e-21	BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD	PF22995	137 - 161	1.9e-14	BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD	PF22992	164 - 202	1.6e-24	BIRD-IDD transcription factor, fourth C2HC zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50700 AT5G66730
fragaria_vesca	FvH4_1g13710 FvH4_1g13710
malus_domestica	MD02G1151700.v1.1 MD15G1266200.v1.1
prunus_persica	Prupe.7G151500_v2.0.a1
pyrus_communis	pycom02g11890 pycom15g23170
rosa_chinensis	RchiOBHm_Chr2g0102031
rosa_laevigata	RLG00000017128
rosa_multiflora	Rmu_co7992216.1_g000001 Rmu_co8011972.1_g000001 Rmu_sc0020987.1_g000001
rosa_roxburghii	Rroxscaffold_2G00140800
rosa_rugosa	Rorug02G0104200.1 Rorug02G0104300
rosa_samantha	Rh2AG152300 Rh2BG157700 Rh2CG157600 Rh2DG157800
rosa_wichuraiana	Rw2G011890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	635
Acc65I	GGTACC	1 cut(s)	482
AccB1I	GGYRCC	1 cut(s)	482
AciI	CCGC	6 cut(s)	106, 443, 802, 1200, 1231, 1476
AclWI	GGATC	2 cut(s)	122, 335
AcsI	RAATTY	3 cut(s)	195, 616, 649
AcuI	CTGAAG	5 cut(s)	18, 339, 666, 693, 1103
AfaI	GTAC	2 cut(s)	484, 850
AfiI	CCNNNNNNNGG	2 cut(s)	1292, 1403
AgsI	TTSAA	4 cut(s)	522, 743, 996, 1332
AjnI	CCWGG	1 cut(s)	485
Alw21I	GWGCWC	2 cut(s)	431, 1511
Alw26I	GTCTC	1 cut(s)	159
AlwI	GGATC	2 cut(s)	122, 335
AlwNI	CAGNNNCTG	1 cut(s)	229
AoxI	GGCC	2 cut(s)	1015, 1405
ApoI	RAATTY	3 cut(s)	195, 616, 649
AseI	ATTAAT	1 cut(s)	1313
Asp700I	GAANNNNTTC	1 cut(s)	1327
Asp718I	GGTACC	1 cut(s)	482
AspLEI	GCGC	2 cut(s)	1081, 1142
AspS9I	GGNCC	4 cut(s)	587, 637, 964, 1340
AsuC2I	CCSGG	1 cut(s)	117
AsuHPI	GGTGA	5 cut(s)	85, 154, 371, 413, 645
AvaII	GGWCC	4 cut(s)	587, 637, 964, 1340
BanI	GGYRCC	1 cut(s)	482
BanII	GRGCYC	1 cut(s)	1511
BbsI	GAAGAC	2 cut(s)	167, 1274
Bbv12I	GWGCWC	2 cut(s)	431, 1511
BbvCI	CCTCAGC	2 cut(s)	570, 918
BccI	CCATC	8 cut(s)	98, 246, 697, 994, 1286, 1296, 1312, 1436
BceAI	ACGGC	1 cut(s)	1125
BcgI	CGANNNNNNTGC	4 cut(s)	171, 205, 221, 255
BciT130I	CCWGG	1 cut(s)	487
BcnI	CCSGG	1 cut(s)	117
BcoDI	GTCTC	1 cut(s)	159
BfaI	CTAG	5 cut(s)	45, 348, 582, 980, 1442
BfmI	CTRYAG	5 cut(s)	230, 814, 1053, 1263, 1455
Bme1390I	CCNGG	2 cut(s)	117, 487
Bme18I	GGWCC	4 cut(s)	587, 637, 964, 1340
BmgT120I	GGNCC	4 cut(s)	587, 637, 964, 1340
BmiI	GGNNCC	2 cut(s)	81, 484
BmrFI	CCNGG	2 cut(s)	117, 487
BmrI	ACTGGG	1 cut(s)	378
BmsI	GCATC	6 cut(s)	124, 550, 772, 1043, 1163, 1472
BmuI	ACTGGG	1 cut(s)	378
BpiI	GAAGAC	2 cut(s)	167, 1274
BplI	GAGNNNNNCTC	2 cut(s)	992, 1024
BpmI	CTGGAG	1 cut(s)	918
Bpu10I	CCTNAGC	3 cut(s)	570, 918, 1344
BpuEI	CTTGAG	2 cut(s)	685, 817
BpuMI	CCSGG	1 cut(s)	117
BsaBI	GATNNNNATC	1 cut(s)	225
BsaJI	CCNNGG	5 cut(s)	260, 486, 951, 1107, 1165
BsaWI	WCCGGW	2 cut(s)	59, 961
Bsc4I	CCNNNNNNNGG	2 cut(s)	1292, 1403
Bse118I	RCCGGY	1 cut(s)	1385
Bse1I	ACTGG	1 cut(s)	373
Bse3DI	GCAATG	2 cut(s)	847, 1226
Bse8I	GATNNNNATC	1 cut(s)	225
BseBI	CCWGG	1 cut(s)	487
BseDI	CCNNGG	5 cut(s)	260, 486, 951, 1107, 1165
BseGI	GGATG	4 cut(s)	689, 1034, 1307, 1424
BseJI	GATNNNNATC	1 cut(s)	225
BseLI	CCNNNNNNNGG	2 cut(s)	1292, 1403
BseMI	GCAATG	2 cut(s)	847, 1226
BseMII	CTCAG	5 cut(s)	561, 608, 734, 812, 932
BseNI	ACTGG	1 cut(s)	373
BseRI	GAGGAG	3 cut(s)	13, 906, 1177
BshFI	GGCC	2 cut(s)	1017, 1407
BshNI	GGYRCC	1 cut(s)	482
BsiHKAI	GWGCWC	2 cut(s)	431, 1511
BsiSI	CCGG	5 cut(s)	60, 77, 117, 962, 1386
BslFI	GGGAC	2 cut(s)	523, 1063
BslI	CCNNNNNNNGG	2 cut(s)	1292, 1403
BsmAI	GTCTC	1 cut(s)	159
BsmFI	GGGAC	2 cut(s)	523, 1063
BsmI	GAATGC	1 cut(s)	126
BsnI	GGCC	2 cut(s)	1017, 1407
Bsp1286I	GDGCHC	2 cut(s)	431, 1511
Bsp143I	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
Bsp19I	CCATGG	1 cut(s)	951
BspACI	CCGC	6 cut(s)	106, 443, 802, 1200, 1231, 1476
BspANI	GGCC	2 cut(s)	1017, 1407
BspCNI	CTCAG	5 cut(s)	562, 607, 733, 811, 931
BspHI	TCATGA	1 cut(s)	1511
BspLI	GGNNCC	2 cut(s)	81, 484
BspMAI	CTGCAG	4 cut(s)	234, 1057, 1267, 1459
BspPI	GGATC	2 cut(s)	122, 335
BspT107I	GGYRCC	1 cut(s)	482
BsrDI	GCAATG	2 cut(s)	847, 1226
BsrFI	RCCGGY	1 cut(s)	1385
BsrI	ACTGG	1 cut(s)	373
BssAI	RCCGGY	1 cut(s)	1385
BssECI	CCNNGG	5 cut(s)	260, 486, 951, 1107, 1165
BssMI	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
BssT1I	CCWWGG	2 cut(s)	260, 951
Bst2UI	CCWGG	1 cut(s)	487
Bst4CI	ACNGT	2 cut(s)	401, 815
Bst6I	CTCTTC	1 cut(s)	293
BstAPI	GCANNNNNTGC	1 cut(s)	989
BstC8I	GCNNGC	1 cut(s)	1217
BstDEI	CTNAG	7 cut(s)	471, 570, 594, 720, 798, 918, 1344
BstDSI	CCRYGG	3 cut(s)	951, 1107, 1165
BstEII	GGTNACC	1 cut(s)	62
BstF5I	GGATG	4 cut(s)	689, 1034, 1307, 1424
BstHHI	GCGC	2 cut(s)	1081, 1142
BstKTI	GATC	6 cut(s)	130, 223, 281, 343, 364, 1363
BstMAI	GTCTC	1 cut(s)	159
BstMBI	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
BstNI	CCWGG	1 cut(s)	487
BstNSI	RCATGY	1 cut(s)	1219
BstPI	GGTNACC	1 cut(s)	62
BstSCI	CCNGG	2 cut(s)	115, 485
BstSFI	CTRYAG	5 cut(s)	230, 814, 1053, 1263, 1455
BstV2I	GAAGAC	2 cut(s)	167, 1274
BstX2I	RGATCY	1 cut(s)	127
BstYI	RGATCY	1 cut(s)	127
BsuRI	GGCC	2 cut(s)	1017, 1407
BtgI	CCRYGG	3 cut(s)	951, 1107, 1165
BtsCI	GGATG	4 cut(s)	689, 1034, 1307, 1424
BtsI	GCAGTG	1 cut(s)	865
BtsIMutI	CAGTG	4 cut(s)	756, 811, 865, 1438
Cac8I	GCNNGC	1 cut(s)	1217
CaiI	CAGNNNCTG	1 cut(s)	229
CciI	TCATGA	1 cut(s)	1511
CfoI	GCGC	2 cut(s)	1081, 1142
Cfr10I	RCCGGY	1 cut(s)	1385
Cfr13I	GGNCC	4 cut(s)	587, 637, 964, 1340
CseI	GACGC	1 cut(s)	319
Csp6I	GTAC	2 cut(s)	483, 849
CviAII	CATG	5 cut(s)	338, 952, 1031, 1216, 1512
CviQI	GTAC	2 cut(s)	483, 849
DdeI	CTNAG	7 cut(s)	471, 570, 594, 720, 798, 918, 1344
DpnI	GATC	6 cut(s)	129, 222, 280, 342, 363, 1362
DpnII	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
DrdI	GACNNNNNNGTC	1 cut(s)	635
DseDI	GACNNNNNNGTC	1 cut(s)	635
Eam1104I	CTCTTC	1 cut(s)	293
EarI	CTCTTC	1 cut(s)	293
Ecl136II	GAGCTC	1 cut(s)	1509
Eco130I	CCWWGG	2 cut(s)	260, 951
Eco24I	GRGCYC	1 cut(s)	1511
Eco47I	GGWCC	4 cut(s)	587, 637, 964, 1340
Eco53kI	GAGCTC	1 cut(s)	1509
Eco57I	CTGAAG	5 cut(s)	18, 339, 666, 693, 1103
Eco91I	GGTNACC	1 cut(s)	62
EcoICRI	GAGCTC	1 cut(s)	1509
EcoO109I	RGGNCCY	1 cut(s)	587
EcoO65I	GGTNACC	1 cut(s)	62
EcoRI	GAATTC	1 cut(s)	616
EcoRII	CCWGG	1 cut(s)	485
EcoT14I	CCWWGG	2 cut(s)	260, 951
EcoT22I	ATGCAT	2 cut(s)	1036, 1156
EcoT38I	GRGCYC	1 cut(s)	1511
ErhI	CCWWGG	2 cut(s)	260, 951
FaeI	CATG	5 cut(s)	341, 955, 1034, 1219, 1515
FalI	AAGNNNNNCTT	4 cut(s)	95, 127, 368, 400
FaqI	GGGAC	2 cut(s)	523, 1063
FatI	CATG	5 cut(s)	337, 951, 1030, 1215, 1511
FokI	GGATG	4 cut(s)	676, 1021, 1314, 1411
FriOI	GRGCYC	1 cut(s)	1511
FspBI	CTAG	5 cut(s)	45, 348, 582, 980, 1442
GlaI	GCGC	2 cut(s)	1080, 1141
GsuI	CTGGAG	1 cut(s)	918
HaeIII	GGCC	2 cut(s)	1017, 1407
HapII	CCGG	5 cut(s)	60, 77, 117, 962, 1386
HgaI	GACGC	1 cut(s)	319
HhaI	GCGC	2 cut(s)	1081, 1142
Hin1II	CATG	5 cut(s)	341, 955, 1034, 1219, 1515
Hin6I	GCGC	2 cut(s)	1079, 1140
HinP1I	GCGC	2 cut(s)	1079, 1140
HinfI	GANTC	7 cut(s)	226, 303, 598, 623, 796, 1225, 1241
HpaII	CCGG	5 cut(s)	60, 77, 117, 962, 1386
HphI	GGTGA	5 cut(s)	85, 154, 371, 413, 645
Hpy166II	GTNNAC	1 cut(s)	334
Hpy188III	TCNNGA	7 cut(s)	131, 295, 348, 897, 1299, 1442, 1512
Hpy8I	GTNNAC	1 cut(s)	334
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	4 cut(s)	354, 600, 1300, 1338
HpyCH4III	ACNGT	2 cut(s)	401, 815
HpyF3I	CTNAG	7 cut(s)	471, 570, 594, 720, 798, 918, 1344
Hsp92II	CATG	5 cut(s)	341, 955, 1034, 1219, 1515
HspAI	GCGC	2 cut(s)	1079, 1140
KpnI	GGTACC	1 cut(s)	486
Kzo9I	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
LmnI	GCTCC	8 cut(s)	79, 85, 240, 718, 899, 1063, 1167, 1506
LweI	GCATC	6 cut(s)	124, 550, 772, 1043, 1163, 1472
MaeI	CTAG	5 cut(s)	45, 348, 582, 980, 1442
MaeIII	GTNAC	2 cut(s)	62, 793
MalI	GATC	6 cut(s)	129, 222, 280, 342, 363, 1362
MboI	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
MfeI	CAATTG	1 cut(s)	1257
MflI	RGATCY	1 cut(s)	127
MhlI	GDGCHC	2 cut(s)	431, 1511
MluCI	AATT	9 cut(s)	195, 616, 649, 729, 942, 1041, 1257, 1310, 1419
MlyI	GAGTC	3 cut(s)	312, 592, 790
MmeI	TCCRAC	1 cut(s)	432
Mph1103I	ATGCAT	2 cut(s)	1036, 1156
MroXI	GAANNNNTTC	1 cut(s)	1327
MseI	TTAA	3 cut(s)	375, 941, 1313
MspA1I	CMGCKG	3 cut(s)	802, 1231, 1460
MspI	CCGG	5 cut(s)	60, 77, 117, 962, 1386
MspR9I	CCNGG	2 cut(s)	117, 487
MunI	CAATTG	1 cut(s)	1257
Mva1269I	GAATGC	1 cut(s)	126
MvaI	CCWGG	1 cut(s)	487
NciI	CCSGG	1 cut(s)	117
NcoI	CCATGG	1 cut(s)	951
NdeII	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
NlaIII	CATG	5 cut(s)	341, 955, 1034, 1219, 1515
NlaIV	GGNNCC	2 cut(s)	81, 484
NmuCI	GTSAC	1 cut(s)	793
NsiI	ATGCAT	2 cut(s)	1036, 1156
NspI	RCATGY	1 cut(s)	1219
PaeI	GCATGC	1 cut(s)	1219
PagI	TCATGA	1 cut(s)	1511
PctI	GAATGC	1 cut(s)	126
PdmI	GAANNNNTTC	1 cut(s)	1327
PfeI	GAWTC	4 cut(s)	226, 623, 1225, 1241
PflFI	GACNNNGTC	1 cut(s)	1278
PleI	GAGTC	3 cut(s)	311, 592, 790
PpsI	GAGTC	3 cut(s)	311, 592, 790
PpuMI	RGGWCCY	1 cut(s)	587
PshBI	ATTAAT	1 cut(s)	1313
Psp124BI	GAGCTC	1 cut(s)	1511
Psp5II	RGGWCCY	1 cut(s)	587
Psp6I	CCWGG	1 cut(s)	485
PspEI	GGTNACC	1 cut(s)	62
PspGI	CCWGG	1 cut(s)	485
PspN4I	GGNNCC	2 cut(s)	81, 484
PspPI	GGNCC	4 cut(s)	587, 637, 964, 1340
PspPPI	RGGWCCY	1 cut(s)	587
PstI	CTGCAG	4 cut(s)	234, 1057, 1267, 1459
PstNI	CAGNNNCTG	1 cut(s)	229
PsuI	RGATCY	1 cut(s)	127
PsyI	GACNNNGTC	1 cut(s)	1278
PvuII	CAGCTG	1 cut(s)	1460
RsaI	GTAC	2 cut(s)	484, 850
RsaNI	GTAC	2 cut(s)	483, 849
SacI	GAGCTC	1 cut(s)	1511
SaqAI	TTAA	3 cut(s)	375, 941, 1313
Sau3AI	GATC	6 cut(s)	127, 220, 278, 340, 361, 1360
Sau96I	GGNCC	4 cut(s)	587, 637, 964, 1340
SchI	GAGTC	3 cut(s)	312, 592, 790
ScrFI	CCNGG	2 cut(s)	117, 487
SduI	GDGCHC	2 cut(s)	431, 1511
SfaNI	GCATC	6 cut(s)	124, 550, 772, 1043, 1163, 1472
SfcI	CTRYAG	5 cut(s)	230, 814, 1053, 1263, 1455
SinI	GGWCC	4 cut(s)	587, 637, 964, 1340
SmlI	CTYRAG	2 cut(s)	700, 832
SmoI	CTYRAG	2 cut(s)	700, 832
SphI	GCATGC	1 cut(s)	1219
Sse9I	AATT	9 cut(s)	195, 616, 649, 729, 942, 1041, 1257, 1310, 1419
SsiI	CCGC	6 cut(s)	106, 443, 802, 1200, 1231, 1476
SspMI	CTAG	5 cut(s)	45, 348, 582, 980, 1442
SstI	GAGCTC	1 cut(s)	1511
StyD4I	CCNGG	2 cut(s)	115, 485
StyI	CCWWGG	2 cut(s)	260, 951
TaaI	ACNGT	2 cut(s)	401, 815
TaqI	TCGA	5 cut(s)	431, 614, 887, 946, 999
TasI	AATT	9 cut(s)	195, 616, 649, 729, 942, 1041, 1257, 1310, 1419
TatI	WGTACW	1 cut(s)	848
TauI	GCSGC	1 cut(s)	1234
TfiI	GAWTC	4 cut(s)	226, 623, 1225, 1241
Tru1I	TTAA	3 cut(s)	375, 941, 1313
Tru9I	TTAA	3 cut(s)	375, 941, 1313
TscAI	CASTG	4 cut(s)	756, 818, 865, 1438
TseFI	GTSAC	1 cut(s)	793
Tsp45I	GTSAC	1 cut(s)	793
TspDTI	ATGAA	2 cut(s)	526, 873
TspGWI	ACGGA	2 cut(s)	23, 1154
TspRI	CASTG	4 cut(s)	756, 818, 865, 1438
Tth111I	GACNNNGTC	1 cut(s)	1278
VpaK11BI	GGWCC	4 cut(s)	587, 637, 964, 1340
VspI	ATTAAT	1 cut(s)	1313
XapI	RAATTY	3 cut(s)	195, 616, 649
XbaI	TCTAGA	2 cut(s)	347, 1441
XceI	RCATGY	1 cut(s)	1219
XmnI	GAANNNNTTC	1 cut(s)	1327
XspI	CTAG	5 cut(s)	45, 348, 582, 980, 1442
Zsp2I	ATGCAT	2 cut(s)	1036, 1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.