FvH4_1g19321

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010487
Physical Location & Seq
Forward (+)
11559909 .. 11562919
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19321.t1

Sequence Viewer

Length: 999 bp
ATGCAATGCACAAATATCATCCCAGTGCCCACAATTCACCCCAAAAAGCTCAAACTTAGGAATTCAAACTCACAACTATCAGTTACTCATCCTCAGAGCAACTTTGAAGTTCAGCTTCCTCTGCCTTCCCATGTCAAATTCATAAGCAGCACTTCAAACCCATTTGTGAAACACTGCCACAAGCTTCGCCAGTCTTCTTCTTATCGCCACTCTCACGGTTCAGTTCTAGTTGTGGGTGCCACACCCATCAGGGAAATATGCGAGTTTCAGAAGTCATTGCTAGAGAAAACTGTTATGATGGATTGTTTGCTTCTACCGGATAATTGTGAGATTCCCGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTGCGGTGATTAAAAAGGTTTCCGGGATGCAATCAACTGAATCTATAGAAGCAATGGCTGTAATGAGAATACCTACGAGTTTTTCAGATATCAATGCTGATGCAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTCTGGTCCTTGACGGTGTTCAGGATCCGGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCGGGGTGCTGTGACCCATTCAATGAAAAAGCACTCCGAGCTAGCCGAGGAGCCTCGTTCCAGCTCCCTATTGTTTCTGGCACTTGGAATCATCTTGAATCCCTCATAACTGAATTCCAAATAAAGATGCTAGCCGGCCATCCAGAGACAGATAAATACTCGAAGCCGTTTCACCTTTCTCAAAAACTTGCAGATTCTCTTGCAAAGCTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTTTGTCTGAGCAATCTCGACGGCATTGTGAGCTTGTAACCATTCCAATGGCAGGAGAGTTTGAGTCACTTAATGTTTCAGTTGCTGGTGGGATCTTCTTGTATATGTTACAGCCTCAAAACCATAAATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

333

Amino Acids

36.59

Weight (kDa)

8.07

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 176 - 322 1e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 236
AciI CCGC 1 cut(s) 383
AclWI GGATC 3 cut(s) 545, 558, 962
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 3 cut(s) 61, 137, 731
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 1 cut(s) 914
AgsI TTSAA 5 cut(s) 66, 107, 156, 640, 716
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 3 cut(s) 545, 558, 962
AlwNI CAGNNNCTG 1 cut(s) 947
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 2 cut(s) 147, 826
ApoI RAATTY 3 cut(s) 61, 137, 731
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 3 cut(s) 403, 555, 621
AsuHPI GGTGA 3 cut(s) 29, 397, 782
AsuNHI GCTAGC 2 cut(s) 659, 748
AvaII GGWCC 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 30
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 2 cut(s) 159, 813
BccI CCATC 5 cut(s) 254, 292, 520, 591, 765
BceAI ACGGC 2 cut(s) 769, 899
BcnI CCSGG 3 cut(s) 403, 555, 621
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 4 cut(s) 227, 281, 660, 749
BfmI CTRYAG 3 cut(s) 423, 496, 585
BisI GCNGC 2 cut(s) 148, 827
BlsI GCNGC 2 cut(s) 149, 828
Bme1390I CCNGG 3 cut(s) 403, 555, 621
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 4 cut(s) 238, 509, 552, 670
BmrFI CCNGG 3 cut(s) 403, 555, 621
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 396, 469, 735
BmtI GCTAGC 2 cut(s) 663, 752
BmuI ACTGGG 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 186
BpuMI CCSGG 3 cut(s) 403, 555, 621
BsaJI CCNNGG 2 cut(s) 620, 664
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 914
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 2 cut(s) 23, 190
Bse3DI GCAATG 3 cut(s) 11, 275, 438
BseDI CCNNGG 2 cut(s) 620, 664
BseGI GGATG 5 cut(s) 18, 88, 411, 602, 757
BseLI CCNNNNNNNGG 1 cut(s) 914
BseMI GCAATG 3 cut(s) 11, 275, 438
BseMII CTCAG 4 cut(s) 107, 589, 847, 861
BseNI ACTGG 2 cut(s) 23, 190
BseRI GAGGAG 1 cut(s) 681
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 159, 813
BshFI GGCC 1 cut(s) 756
BshNI GGYRCC 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 5 cut(s) 317, 402, 554, 620, 753
BslI CCNNNNNNNGG 1 cut(s) 914
BsmAI GTCTC 1 cut(s) 758
BsnI GGCC 1 cut(s) 756
Bsp1286I GDGCHC 2 cut(s) 30, 380
Bsp143I GATC 3 cut(s) 550, 578, 954
BspACI CCGC 1 cut(s) 383
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 4 cut(s) 106, 588, 846, 862
BspLI GGNNCC 4 cut(s) 238, 509, 552, 670
BspOI GCTAGC 2 cut(s) 663, 752
BspPI GGATC 3 cut(s) 545, 558, 962
BspT107I GGYRCC 1 cut(s) 236
BsrDI GCAATG 3 cut(s) 11, 275, 438
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 2 cut(s) 23, 190
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 2 cut(s) 620, 664
BssMI GATC 3 cut(s) 550, 578, 954
Bst4CI ACNGT 4 cut(s) 218, 292, 497, 542
BstC8I GCNNGC 3 cut(s) 661, 750, 754
BstDEI CTNAG 5 cut(s) 56, 93, 575, 833, 870
BstF5I GGATG 5 cut(s) 18, 88, 411, 602, 757
BstKTI GATC 3 cut(s) 553, 581, 957
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 3 cut(s) 550, 578, 954
BstMWI GCNNNNNNNGC 4 cut(s) 121, 488, 656, 892
BstSCI CCNGG 3 cut(s) 401, 553, 619
BstSFI CTRYAG 3 cut(s) 423, 496, 585
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 159, 813
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 550, 954
BstXI CCANNNNNNTGG 1 cut(s) 910
BstYI RGATCY 2 cut(s) 550, 954
BsuRI GGCC 1 cut(s) 756
BtsCI GGATG 5 cut(s) 18, 88, 411, 602, 757
BtsI GCAGTG 2 cut(s) 172, 569
BtsIMutI CAGTG 3 cut(s) 30, 172, 569
Cac8I GCNNGC 3 cut(s) 661, 750, 754
CaiI CAGNNNCTG 1 cut(s) 947
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 567
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 1 cut(s) 131
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 5 cut(s) 56, 93, 575, 833, 870
DpnI GATC 3 cut(s) 552, 580, 956
DpnII GATC 3 cut(s) 550, 578, 954
EaeI YGGCCR 1 cut(s) 754
Ecl136II GAGCTC 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 380
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 378
EcoRI GAATTC 2 cut(s) 61, 731
EcoRV GATATC 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 380
FaeI CATG 1 cut(s) 134
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 136, 168
FatI CATG 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 148, 827
FokI GGATG 5 cut(s) 5, 75, 418, 609, 744
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 2 cut(s) 148, 827
FspBI CTAG 4 cut(s) 227, 281, 660, 749
GluI GCNGC 2 cut(s) 148, 827
HaeIII GGCC 1 cut(s) 756
HapII CCGG 5 cut(s) 317, 402, 554, 620, 753
Hin1II CATG 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 6 cut(s) 331, 419, 706, 716, 812, 926
HpaII CCGG 5 cut(s) 317, 402, 554, 620, 753
HphI GGTGA 3 cut(s) 29, 397, 782
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 6 cut(s) 96, 270, 466, 578, 656, 871
Hpy188III TCNNGA 5 cut(s) 335, 548, 713, 761, 879
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 1 cut(s) 885
HpyAV CCTTC 2 cut(s) 135, 848
HpyCH4III ACNGT 4 cut(s) 218, 292, 497, 542
HpyCH4V TGCA 7 cut(s) 4, 9, 370, 409, 482, 809, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 488, 656, 892
HpyF3I CTNAG 5 cut(s) 56, 93, 575, 833, 870
Hsp92II CATG 1 cut(s) 134
KroI GCCGGC 1 cut(s) 752
KroNI GCCGGC 1 cut(s) 754
Kzo9I GATC 3 cut(s) 550, 578, 954
LmnI GCTCC 2 cut(s) 668, 687
Lsp1109I GCAGC 2 cut(s) 159, 813
LweI GCATC 3 cut(s) 396, 469, 735
MaeI CTAG 4 cut(s) 227, 281, 660, 749
MaeIII GTNAC 5 cut(s) 82, 629, 898, 927, 969
MalI GATC 3 cut(s) 552, 580, 956
MboI GATC 3 cut(s) 550, 578, 954
MboII GAAGA 5 cut(s) 186, 189, 605, 608, 949
MflI RGATCY 2 cut(s) 550, 954
MhlI GDGCHC 2 cut(s) 30, 380
MluCI AATT 5 cut(s) 33, 61, 137, 322, 731
MlyI GAGTC 1 cut(s) 935
MnlI CCTC 7 cut(s) 102, 129, 481, 659, 682, 731, 987
MroNI GCCGGC 1 cut(s) 752
MseI TTAA 2 cut(s) 390, 933
MslI CAYNNNNRTG 2 cut(s) 23, 908
MspI CCGG 5 cut(s) 317, 402, 554, 620, 753
MspR9I CCNGG 3 cut(s) 403, 555, 621
MwoI GCNNNNNNNGC 4 cut(s) 121, 488, 656, 892
NaeI GCCGGC 1 cut(s) 754
NciI CCSGG 3 cut(s) 403, 555, 621
NdeII GATC 3 cut(s) 550, 578, 954
NgoMIV GCCGGC 1 cut(s) 752
NheI GCTAGC 2 cut(s) 659, 748
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 4 cut(s) 238, 509, 552, 670
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 2 cut(s) 629, 927
PdiI GCCGGC 1 cut(s) 754
PfeI GAWTC 5 cut(s) 331, 419, 706, 716, 812
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 2 cut(s) 149, 828
PleI GAGTC 1 cut(s) 934
PpsI GAGTC 1 cut(s) 934
Psp124BI GAGCTC 1 cut(s) 380
PspN4I GGNNCC 4 cut(s) 238, 509, 552, 670
PspPI GGNCC 1 cut(s) 532
PstNI CAGNNNCTG 1 cut(s) 947
PsuI RGATCY 2 cut(s) 550, 954
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 2 cut(s) 23, 908
SacI GAGCTC 1 cut(s) 380
SaqAI TTAA 2 cut(s) 390, 933
SatI GCNGC 2 cut(s) 148, 827
Sau3AI GATC 3 cut(s) 550, 578, 954
Sau96I GGNCC 1 cut(s) 532
SchI GAGTC 1 cut(s) 935
ScrFI CCNGG 3 cut(s) 403, 555, 621
SduI GDGCHC 2 cut(s) 30, 380
SfaNI GCATC 3 cut(s) 396, 469, 735
SfcI CTRYAG 3 cut(s) 423, 496, 585
SinI GGWCC 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 23, 908
Sse9I AATT 5 cut(s) 33, 61, 137, 322, 731
SsiI CCGC 1 cut(s) 383
SspMI CTAG 4 cut(s) 227, 281, 660, 749
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 401, 553, 619
TaaI ACNGT 4 cut(s) 218, 292, 497, 542
TaqI TCGA 3 cut(s) 523, 779, 880
TasI AATT 5 cut(s) 33, 61, 137, 322, 731
TfiI GAWTC 5 cut(s) 331, 419, 706, 716, 812
Tru1I TTAA 2 cut(s) 390, 933
Tru9I TTAA 2 cut(s) 390, 933
TscAI CASTG 3 cut(s) 30, 179, 576
TseFI GTSAC 2 cut(s) 629, 927
TseI GCWGC 2 cut(s) 147, 826
Tsp45I GTSAC 2 cut(s) 629, 927
TspDTI ATGAA 2 cut(s) 130, 657
TspRI CASTG 3 cut(s) 30, 179, 576
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 3 cut(s) 61, 137, 731
XspI CTAG 4 cut(s) 227, 281, 660, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.