RLG00000017832

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010487
Physical Location & Seq
Forward (+)
21901209 .. 21903705
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017832

Sequence Viewer

Length: 1014 bp
ATGGAATGCACAAGTATCATCTCAGTGCCCACAATCCACTCCAGAAAGGTCAAACTTAGGAGGAACTCACACTCACAATTATCAGTTGCTCATCCTCAGAGCAGCTTTGAAGACTATGATGATGTGAAGCTTCCTCTGCCTTCCCATGTCAAATTCATAACCAGCACTTCAAACCCATTCGTCAAACACTGCCACAAGCTTCGCCTGTCTTCTTCTTACCGCCACACTCATGGTTCAGTTCTCGTTGTGGGTGCTACTCCTATCAGGGAAATATGGGAGTTTCAGAAGTCATTGCAAGAGAAAACTGTTATGATGGATTGTTTGCTTGTACCGGATAATTGTGAGGTTCCTGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTCCTGTGATGAAAAAGCTTTCGGGGATGCAATCAACTGATTCTATTGAAGCAATGGCTCTAATGAGAATACCTACGAGTTTTTCAGATATCAAAGCTGATGAAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTATGGTCCTTGATGGTGTTCAGGATCCGGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCCGGGTGTTGTGATCCATTCAATGAAAAAGCACTCCGAGCTAGCCGAGGAGCATCATTCCAGCTTCCTATTATTTCTGGAACTTGGAGTCATCTTGAATCCCTCATAAGTGAATTCCAAATAAAGATGCTAGCTGGCCATCCAGAGACTGATAAAAAGTCGACGCCATTTCACCTTTCTCAAAAACTTGCAGATTCTCTTGCAACAGTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTCTGTCTGAGCAATCTCGACGGCAATGTGAGCTTGTAACCATTCCAATGGCAGGAGATTTTGAGTCGCTTAATGTTTCAGTTGCTGGTGGGATTTTCTTGTATATGTTGCAACCTAAAAACCGTATATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

338

Amino Acids

37.27

Weight (kDa)

7.18

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 181 - 327 9.4e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 794
AciI CCGC 1 cut(s) 220
AclWI GGATC 3 cut(s) 560, 573, 641
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 2 cut(s) 152, 746
AcyI GRCGYC 1 cut(s) 797
AfaI GTAC 2 cut(s) 330, 583
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 5 cut(s) 110, 171, 443, 655, 731
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 773
AlwI GGATC 3 cut(s) 560, 573, 641
AlwNI CAGNNNCTG 2 cut(s) 782, 962
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 1 cut(s) 102
ApoI RAATTY 2 cut(s) 152, 746
AspS9I GGNCC 1 cut(s) 547
AsuC2I CCSGG 3 cut(s) 570, 636, 637
AsuHPI GGTGA 1 cut(s) 797
AsuNHI GCTAGC 2 cut(s) 674, 763
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 1 cut(s) 547
BaeGI GKGCMC 1 cut(s) 30
BalI TGGCCA 1 cut(s) 771
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 2 cut(s) 117, 201
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 1 cut(s) 114
BccI CCATC 5 cut(s) 307, 535, 548, 606, 780
BceAI ACGGC 1 cut(s) 914
BcnI CCSGG 3 cut(s) 570, 636, 637
BcoDI GTCTC 1 cut(s) 773
BfaI CTAG 2 cut(s) 675, 764
BfmI CTRYAG 2 cut(s) 511, 600
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
Bme1390I CCNGG 3 cut(s) 570, 636, 637
Bme18I GGWCC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 3 cut(s) 348, 524, 567
BmrFI CCNGG 3 cut(s) 570, 636, 637
BmsI GCATC 3 cut(s) 411, 695, 750
BmtI GCTAGC 2 cut(s) 678, 767
BpiI GAAGAC 2 cut(s) 117, 201
BpmI CTGGAG 1 cut(s) 25
BpuMI CCSGG 3 cut(s) 570, 636, 637
BsaHI GRCGYC 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 635, 679
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse3DI GCAATG 3 cut(s) 290, 453, 908
BseDI CCNNGG 2 cut(s) 635, 679
BseGI GGATG 4 cut(s) 91, 426, 617, 772
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 3 cut(s) 290, 453, 908
BseMII CTCAG 5 cut(s) 36, 110, 604, 862, 876
BseRI GAGGAG 1 cut(s) 696
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 1 cut(s) 114
BshFI GGCC 1 cut(s) 771
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 332, 569, 636
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 1 cut(s) 773
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 1 cut(s) 771
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 30, 395
Bsp143I GATC 3 cut(s) 565, 593, 646
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 5 cut(s) 35, 109, 603, 861, 877
BspLI GGNNCC 3 cut(s) 348, 524, 567
BspOI GCTAGC 2 cut(s) 678, 767
BspPI GGATC 3 cut(s) 560, 573, 641
BsrDI GCAATG 3 cut(s) 290, 453, 908
BssECI CCNNGG 2 cut(s) 635, 679
BssMI GATC 3 cut(s) 565, 593, 646
BssNI GRCGYC 1 cut(s) 797
Bst4CI ACNGT 4 cut(s) 307, 512, 841, 1001
BstACI GRCGYC 1 cut(s) 797
BstC8I GCNNGC 3 cut(s) 676, 765, 769
BstDEI CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
BstF5I GGATG 4 cut(s) 91, 426, 617, 772
BstKTI GATC 3 cut(s) 568, 596, 649
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 3 cut(s) 565, 593, 646
BstMWI GCNNNNNNNGC 3 cut(s) 136, 671, 907
BstSCI CCNGG 3 cut(s) 568, 634, 635
BstSFI CTRYAG 2 cut(s) 511, 600
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 2 cut(s) 117, 201
BstX2I RGATCY 1 cut(s) 565
BstXI CCANNNNNNTGG 2 cut(s) 230, 925
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 1 cut(s) 771
BtsCI GGATG 4 cut(s) 91, 426, 617, 772
BtsI GCAGTG 2 cut(s) 187, 584
BtsIMutI CAGTG 4 cut(s) 30, 187, 584, 846
Cac8I GCNNGC 3 cut(s) 676, 765, 769
CaiI CAGNNNCTG 2 cut(s) 782, 962
Cfr13I GGNCC 1 cut(s) 547
Cfr9I CCCGGG 1 cut(s) 635
CseI GACGC 1 cut(s) 805
Csp6I GTAC 2 cut(s) 329, 582
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviAII CATG 2 cut(s) 146, 230
CviQI GTAC 2 cut(s) 329, 582
DdeI CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
DpnI GATC 3 cut(s) 567, 595, 648
DpnII GATC 3 cut(s) 565, 593, 646
EaeI YGGCCR 1 cut(s) 769
Ecl136II GAGCTC 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 395
Eco32I GATATC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 746
EcoRV GATATC 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 2 cut(s) 149, 233
FatI CATG 2 cut(s) 145, 229
FblI GTMKAC 1 cut(s) 794
Fnu4HI GCNGC 1 cut(s) 103
FokI GGATG 4 cut(s) 78, 433, 624, 759
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 2 cut(s) 675, 764
GluI GCNGC 1 cut(s) 103
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 1 cut(s) 771
HapII CCGG 3 cut(s) 332, 569, 636
HgaI GACGC 1 cut(s) 805
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 2 cut(s) 149, 233
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 3 cut(s) 128, 197, 410
HinfI GANTC 5 cut(s) 434, 721, 731, 827, 941
HpaII CCGG 3 cut(s) 332, 569, 636
HphI GGTGA 1 cut(s) 797
Hpy166II GTNNAC 2 cut(s) 584, 795
Hpy188I TCNGA 6 cut(s) 99, 285, 481, 593, 671, 886
Hpy188III TCNNGA 7 cut(s) 42, 350, 563, 711, 728, 776, 894
Hpy8I GTNNAC 2 cut(s) 584, 795
Hpy99I CGWCG 2 cut(s) 799, 900
HpyAV CCTTC 2 cut(s) 150, 863
HpyCH4III ACNGT 4 cut(s) 307, 512, 841, 1001
HpyCH4V TGCA 7 cut(s) 9, 295, 385, 424, 824, 836, 988
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 671, 907
HpyF3I CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 2 cut(s) 149, 233
Kzo9I GATC 3 cut(s) 565, 593, 646
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 3 cut(s) 411, 695, 750
MaeI CTAG 2 cut(s) 675, 764
MaeIII GTNAC 1 cut(s) 913
MalI GATC 3 cut(s) 567, 595, 648
MboI GATC 3 cut(s) 565, 593, 646
MboII GAAGA 5 cut(s) 122, 201, 204, 620, 623
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 2 cut(s) 30, 395
MlsI TGGCCA 1 cut(s) 771
MluCI AATT 4 cut(s) 77, 152, 337, 746
MluNI TGGCCA 1 cut(s) 771
MlyI GAGTC 2 cut(s) 730, 950
MnlI CCTC 7 cut(s) 54, 105, 144, 337, 496, 674, 746
Mox20I TGGCCA 1 cut(s) 771
MscI TGGCCA 1 cut(s) 771
MseI TTAA 1 cut(s) 948
MslI CAYNNNNRTG 3 cut(s) 23, 228, 923
Msp20I TGGCCA 1 cut(s) 771
MspI CCGG 3 cut(s) 332, 569, 636
MspR9I CCNGG 3 cut(s) 570, 636, 637
Mva1269I GAATGC 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 136, 671, 907
NciI CCSGG 3 cut(s) 570, 636, 637
NdeII GATC 3 cut(s) 565, 593, 646
NheI GCTAGC 2 cut(s) 674, 763
NlaIII CATG 2 cut(s) 149, 233
NlaIV GGNNCC 3 cut(s) 348, 524, 567
NmeAIII GCCGAG 1 cut(s) 704
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 3 cut(s) 434, 731, 827
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 2 cut(s) 729, 949
PpsI GAGTC 2 cut(s) 729, 949
Psp124BI GAGCTC 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 348, 524, 567
PspPI GGNCC 1 cut(s) 547
PstNI CAGNNNCTG 2 cut(s) 782, 962
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 2 cut(s) 330, 583
RsaNI GTAC 2 cut(s) 329, 582
RseI CAYNNNNRTG 3 cut(s) 23, 228, 923
SacI GAGCTC 1 cut(s) 395
SalI GTCGAC 1 cut(s) 793
SaqAI TTAA 1 cut(s) 948
SatI GCNGC 1 cut(s) 103
Sau3AI GATC 3 cut(s) 565, 593, 646
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 2 cut(s) 730, 950
ScrFI CCNGG 3 cut(s) 570, 636, 637
SduI GDGCHC 2 cut(s) 30, 395
SfaNI GCATC 3 cut(s) 411, 695, 750
SfcI CTRYAG 2 cut(s) 511, 600
SinI GGWCC 1 cut(s) 547
SmaI CCCGGG 1 cut(s) 637
SmiMI CAYNNNNRTG 3 cut(s) 23, 228, 923
Sse9I AATT 4 cut(s) 77, 152, 337, 746
SsiI CCGC 1 cut(s) 220
SspMI CTAG 2 cut(s) 675, 764
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 568, 634, 635
TaaI ACNGT 4 cut(s) 307, 512, 841, 1001
TaqI TCGA 3 cut(s) 538, 794, 895
TasI AATT 4 cut(s) 77, 152, 337, 746
TfiI GAWTC 3 cut(s) 434, 731, 827
Tru1I TTAA 1 cut(s) 948
Tru9I TTAA 1 cut(s) 948
TscAI CASTG 4 cut(s) 30, 194, 591, 846
TseI GCWGC 1 cut(s) 102
TspDTI ATGAA 4 cut(s) 145, 419, 510, 672
TspMI CCCGGG 1 cut(s) 635
TspRI CASTG 4 cut(s) 30, 194, 591, 846
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 2 cut(s) 152, 746
XmaI CCCGGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 794
XspI CTAG 2 cut(s) 675, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.