Rorug02G0168800

Aldehyde dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000503
Physical Location & Seq
Reverse (-)
15146213 .. 15153519
7307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0168800.1

Sequence Viewer

Length: 3828 bp
ATGGCATTATCATTTGCACTGGATTTAATCGGAGGACAAGTATTTGAGCTGTTGTCTGCAAGTGTTACGCGAGCGGTGGACAGGAAAGCTGCGTTTGGAGACCTTCTCCAAGATATGAAATACACACTGAAGTCCTTGAAAAGACAGGCCATAGTGATTCAACAGATACAAGAGTATAATCTGGAACTGAGTCTCCCAAATGATGAAATGATTGCTCTTGTAACACAAATGACAGTTGGAAGACAACTTGTTGATAAGTTGTCAGGGATTAGATTTCGTTTGTGCAACTTCTGCTGCATAAATGATTTGGCTGACCAACTTAAAGAATTGAATAGTTCTCTTGAAAGTCTGTTTGACAACCTGCAGTTGGAGCAAGTAAGGGATATTAAGGAGACCTTGAAATTGTCAAGGCAAAACCGCGATGATCTTGAGAAAATGAAGGTCATGTTGGAGAAGATAATGCAGATGATTGATGATCCTGAAGTGAAGGGTCTACTGAAGAAGTTAGTGGAGCAGAATGCAGGCGGATCAATGAAGAGCTCGCCCTCAACCTCAACCTTGGGCTCAGGAAGTTGCACAGAAAATGAAGGGAATTGCGAAACACAAGTGCCGTCATCAAAAGCACTAGTAGAAGCAGCATTTCGGGTGCTACTTAATGCCGTTATAGAAGTGAAGGAGAGCACTATTATGTTTAGACCTCTCCTCCGACGTCTCAAATCTACACTAGACTATTTACTGCCATTGATTGGAGATATGGTACAATGTAACAAGGCATTAGATCGCCCAAAAGAGGAACTAGAAAATATGAGAATACAAATGGAGAATGGTGTAGTGCTTGTTGGCAAGTGCTTAAATGTTCGCCGGTGGACAAGAAACAAAAAGTATATATACACCAACCAACTTTTTGAATTGAATGATTATCTTCAAGAGCAGTTAAGTATACTGCTAGTGGAGATAGCAAGGGATGCGAGGGAGACCTCGGTGGTGATAAGGAATATTAAGGACATGGTCAAGAGAGATGAAAGGAGTGGTATGGCAGAAAATCAACTTGAATATAAAGGTTTTTGTAATGTATCTGAACCTCCATTGCCTACTGTTGAACTCGAAACAAACATGCAAGGAACAAGGGATGTGATGATGGAGACATTGGTTTCTGTAAAGAATATCGAGGAGGGGGTCAAGGGAATGGAAGGGAGAATTATAGATGATGTATCTGAAACTCTGTCGCCTATTGTTGGAAGTGAAGTACTCAACATGCAGGGAACAAAGGATGTGATCAAGGAGACACAGGCTTCAACAGAGAATACAAATCCAGAGGTCAACCTAATTGAAGGCAGAGCTGAGTCCCAGTTTGGCTCCGTTGCATCTTCAGAGAGCCAGCAGCAAACAGAGGGAATCAGTAAAGTACATGGAACTTTAGGTCACTGTGAACTGGAAAATTCAGTATCTCAAGAGACCTTTTCACTATACCTTCCTGATTCCATCCCTCTAGAGTTCAATGCATTGAAGAAGGAAACTGTATTTCCTGACAGACCTGGCCAACCTGACTGTCTTGACAAGGAGTACAAAATCAACGGGACAGTGAAAAAGCCTATTTCCTTTAAGGTTCACTGTTCAATGTGCATTCAACTTTGGAAGTTGGTGGAGAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGACCTCGTTGCTCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCTACCTGGCGTTAACAGGGGATGTGATAGTCTCAAGATGTCAAAAATCAAGGAACTTGTTGGAGCAAAGTTTGTGCCAGATTCAGAGTTGGGTTCCAGTGATTTTGGTGGCAGAGATCTCTAAAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAGAAGATTCTGAACTCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCAAAGGAAGTCTTAATAGAGAGAAGATCTATTAAGAACTTGCTACAAAAAGTTGGTGACAGTGACTCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTATTGAAGAAGTATGGAAACTTAATATTGGTAAAGCAAACAGAGAGTGCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGAATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTGTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTGATGAGGGAAATGATACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGACTCCAAACGTTGGCATGACGGAGTTGATATCAAGGTGGTGTACGAAGTATGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTTAGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACTATGTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGATTGATGATGATTCTCTGAAATACCGATCTGCTAAAAAGTGCGAGACAGATTTGGAATTTCTATCTAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACCATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTTTGTTGAACCTCTCATCAAATTTTTGAGGGATGCATATGATCAGAATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAACAGAAACGGGATATCTTATTTAGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAAGAAGGTCTTGACATAATGGAAATTCTGTCTGTCCACCAGGACTGCCGAGGTAACATCTCATCATCTGGTGCACTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTTAAAATTTTGCACAATTTGTCCTTCGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTCGAAGACAGTGCTCTTACTGAAAACTGTATCGCTATATTGAAAAATCTGAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGATGGATGCATTGCTTATGTTGTTGATGTCCTAGACACTGGCAGTAAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGACTATTGTAACTTGGTCATGCATGAGGGTGTTATTCCTGCTCTTGTCCTCTCATCTACCAATGGGACAGAGAGAACAAAGATAAACGCAATGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGTCGATGAGCAAGAATCTGCTGAACCTGAGCCTGAGGCAGCTAGAGATGAGCTACCTAGAGACAATGGAAATCACTCGAACAGGCAGAAAGCTTCTGAGGCATCAGGATTTTTCGCAAAGAAGTTATCAATATTCTCAAAACCGAGTTCTCTTTCACCGAAAAAGAAGAAATGA

Protein Analysis

1275

Amino Acids

142.83

Weight (kDa)

5.22

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 143 1.9e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 208 - 332 1.7e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
U-box PF04564 790 - 857 2.5e-16 U-box domain
ARM_PUB PF25598 954 - 1215 5.7e-15 Plant U-box protein ARM repeat region
KAP PF05804 1003 - 1162 6.9e-08 Kinesin-associated protein (KAP)
Arm_2 PF04826 1021 - 1127 2.1e-07 Armadillo-like
ARM_ARMC5 PF24768 1049 - 1210 2e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14170
fragaria_vesca FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11410 FvH4_2g11410
malus_domestica MD09G1234200.v1.1 MD15G1309700.v1.1
prunus_persica Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1
pyrus_communis pycom09g15250 pycom15g27380
rosa_chinensis RchiOBHm_Chr2g0110691 RchiOBHm_Chr2g0137621 RchiOBHm_Chr6g0270041 RchiOBHm_Chr6g0270061 RchiOBHm_Chr6g0270141
rosa_laevigata RLG00000013815 RLG00000013819 RLG00000017831 RLG00000019609
rosa_multiflora Rmu_co8442727.1_g000001 Rmu_sc0000389.1_g000042 Rmu_sc0000918.1_g000033 Rmu_sc0003043.1_g000019 Rmu_sc0004376.1_g000013
rosa_roxburghii Rroxscaffold_2G00133030 Rroxscaffold_5G00350410 Rroxscaffold_5G00350420 Rroxscaffold_5G00350430 Rroxscaffold_6G00398360 Rroxscaffold_7G00198140 Rroxscaffold_7G00198180
rosa_rugosa Rorug02G0168700 Rorug02G0168800 Rorug02G0168800 Rorug02G0338300 Rorug06G0052100 Rorug06G0052200 Rorug06G0052800 Rorug06G0052900
rosa_samantha Rh2AG221000 Rh2AG387100 Rh2AG387200 Rh2AG547100 Rh2BG231100 Rh2BG504800 Rh2CG223400 Rh2CG374500 Rh2DG227200 Rh2DG410100 Rh2DG516200 Rh5CG251300 Rh6AG171200 Rh6AG171700 Rh6BG175700 Rh6BG176100 Rh6CG170500 Rh6CG171200 Rh6CG216400 Rh6DG162700 Rh6DG163000
rosa_wichuraiana Rw2G017050 Rw6G014700 Rw6G014740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 2 cut(s) 746, 2537
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 4 cut(s) 493, 940, 2683, 3235
AccII CGCG 2 cut(s) 70, 420
AciI CCGC 4 cut(s) 74, 418, 525, 2385
AclI AACGTT 1 cut(s) 2535
AclWI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
AcoI YGGCCR 1 cut(s) 1537
AcuI CTGAAG 6 cut(s) 149, 501, 518, 1353, 1974, 2319
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 7 cut(s) 759, 1248, 1407, 1565, 2364, 2408, 2569
AfiI CCNNNNNNNGG 9 cut(s) 367, 746, 790, 1235, 2235, 2315, 2537, 2869, 3325
AflII CTTAAG 1 cut(s) 1922
AflIII ACRYGT 2 cut(s) 2346, 3075
AhlI ACTAGT 1 cut(s) 625
AjiI CACGTC 1 cut(s) 3076
AjnI CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
Alw21I GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
Alw44I GTGCAC 1 cut(s) 3189
AlwI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
AoxI GGCC 4 cut(s) 147, 1537, 2511, 3402
ApaLI GTGCAC 1 cut(s) 3189
ApeKI GCWGC 8 cut(s) 89, 294, 635, 1381, 1679, 1767, 1928, 3692
AspLEI GCGC 1 cut(s) 3525
AspS9I GGNCC 2 cut(s) 2512, 3402
AsuHPI GGTGA 5 cut(s) 997, 2144, 2575, 3080, 3801
AsuII TTCGAA 1 cut(s) 3339
AxyI CCTNAGG 1 cut(s) 3687
BaeGI GKGCMC 2 cut(s) 2230, 3193
BaeI ACNNNNGTAYC 4 cut(s) 158, 191, 2447, 2480
BalI TGGCCA 1 cut(s) 1539
BamHI GGATCC 1 cut(s) 2714
BanII GRGCYC 3 cut(s) 542, 566, 2850
BbsI GAAGAC 3 cut(s) 247, 3078, 3348
Bbv12I GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
BbvI GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BccI CCATC 8 cut(s) 1132, 1490, 1957, 2594, 2744, 3002, 3292, 3422
BceAI ACGGC 2 cut(s) 595, 644
BcgI CGANNNNNNTGC 2 cut(s) 3329, 3363
BciT130I CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BciVI GTATCC 1 cut(s) 2448
BclI TGATCA 2 cut(s) 1275, 2980
BcuI ACTAGT 1 cut(s) 625
BfmI CTRYAG 1 cut(s) 362
BfrI CTTAAG 1 cut(s) 1922
BfuAI ACCTGC 1 cut(s) 369
BfuI GTATCC 1 cut(s) 2448
BglII AGATCT 3 cut(s) 1899, 2102, 2157
BisI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
BlsI GCNGC 8 cut(s) 91, 296, 637, 1383, 1681, 1769, 1930, 3694
BmcAI AGTACT 1 cut(s) 1248
Bme1390I CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BmgBI CACGTC 1 cut(s) 3076
BmgT120I GGNCC 2 cut(s) 2512, 3402
BmiI GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
BmrFI CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BmrI ACTGGG 1 cut(s) 1342
BmuI ACTGGG 1 cut(s) 1342
BpiI GAAGAC 3 cut(s) 247, 3078, 3348
BplI GAGNNNNNCTC 4 cut(s) 90, 122, 1971, 2003
Bpu10I CCTNAGC 2 cut(s) 565, 3681
Bpu14I TTCGAA 1 cut(s) 3339
BpuEI CTTGAG 7 cut(s) 449, 1434, 1801, 2018, 2355, 2895, 3658
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 4 cut(s) 93, 386, 968, 1448
BsaJI CCNNGG 3 cut(s) 558, 978, 3166
BsaXI ACNNNNNCTCC 8 cut(s) 24, 54, 177, 207, 687, 717, 741, 771
Bsc4I CCNNNNNNNGG 9 cut(s) 367, 746, 790, 1235, 2235, 2315, 2537, 2869, 3325
Bse118I RCCGGY 1 cut(s) 861
Bse1I ACTGG 8 cut(s) 24, 1348, 1437, 1880, 2320, 2514, 2727, 3469
Bse21I CCTNAGG 1 cut(s) 3687
Bse3DI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BseBI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BseDI CCNNGG 3 cut(s) 558, 978, 3166
BseLI CCNNNNNNNGG 9 cut(s) 367, 746, 790, 1235, 2235, 2315, 2537, 2869, 3325
BseMI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BseNI ACTGG 8 cut(s) 24, 1348, 1437, 1880, 2320, 2514, 2727, 3469
BseRI GAGGAG 3 cut(s) 692, 1184, 2358
BseSI GKGCMC 2 cut(s) 2230, 3193
BseXI GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 1 cut(s) 2786
Bsh1236I CGCG 2 cut(s) 70, 420
BshFI GGCC 4 cut(s) 149, 1539, 2513, 3404
BsiHKAI GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
BsiSI CCGG 1 cut(s) 862
BslFI GGGAC 3 cut(s) 1330, 1591, 3607
BslI CCNNNNNNNGG 9 cut(s) 367, 746, 790, 1235, 2235, 2315, 2537, 2869, 3325
BsmBI CGTCTC 2 cut(s) 716, 3638
BsmFI GGGAC 3 cut(s) 1330, 1591, 3607
BsmI GAATGC 2 cut(s) 523, 1623
BsnI GGCC 4 cut(s) 149, 1539, 2513, 3404
Bso31I GGTCTC 4 cut(s) 93, 386, 968, 1448
Bsp119I TTCGAA 1 cut(s) 3339
Bsp1286I GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
BspACI CCGC 4 cut(s) 74, 418, 525, 2385
BspANI GGCC 4 cut(s) 149, 1539, 2513, 3404
BspFNI CGCG 2 cut(s) 70, 420
BspLI GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
BspMAI CTGCAG 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
BspQI GCTCTTC 2 cut(s) 530, 2046
BspT104I TTCGAA 1 cut(s) 3339
BspTI CTTAAG 1 cut(s) 1922
BspTNI GGTCTC 4 cut(s) 93, 386, 968, 1448
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BsrFI RCCGGY 1 cut(s) 861
BsrI ACTGG 8 cut(s) 24, 1348, 1437, 1880, 2320, 2514, 2727, 3469
BssAI RCCGGY 1 cut(s) 861
BssECI CCNNGG 3 cut(s) 558, 978, 3166
BssNAI GTATAC 2 cut(s) 941, 3236
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 558
Bst1107I GTATAC 2 cut(s) 941, 3236
Bst2UI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
Bst6I CTCTTC 4 cut(s) 530, 1953, 2046, 2641
BstACI GRCGYC 1 cut(s) 709
BstAFI CTTAAG 1 cut(s) 1922
BstAPI GCANNNNNTGC 2 cut(s) 291, 965
BstBI TTCGAA 1 cut(s) 3339
BstC8I GCNNGC 5 cut(s) 72, 523, 542, 1379, 3489
BstFNI CGCG 2 cut(s) 70, 420
BstHHI GCGC 1 cut(s) 3525
BstMWI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
BstNI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BstNSI RCATGY 6 cut(s) 1117, 1258, 1723, 2350, 2490, 3491
BstSCI CCNGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
BstSFI CTRYAG 1 cut(s) 362
BstSLI GKGCMC 2 cut(s) 2230, 3193
BstUI CGCG 2 cut(s) 70, 420
BstV1I GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BstV2I GAAGAC 3 cut(s) 247, 3078, 3348
BstX2I RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
BstYI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
BstZ17I GTATAC 2 cut(s) 941, 3236
Bsu36I CCTNAGG 1 cut(s) 3687
BsuI GTATCC 1 cut(s) 2448
BsuRI GGCC 4 cut(s) 149, 1539, 2513, 3404
BtgZI GCGATG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 3076
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 5 cut(s) 72, 523, 542, 1379, 3489
CfoI GCGC 1 cut(s) 3525
Cfr10I RCCGGY 1 cut(s) 861
Cfr13I GGNCC 2 cut(s) 2512, 3402
Csp6I GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
CspCI CAANNNNNGTGG 2 cut(s) 2629, 2664
CviQI GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
DraI TTTAAA 1 cut(s) 3016
EaeI YGGCCR 1 cut(s) 1537
Eam1104I CTCTTC 4 cut(s) 530, 1953, 2046, 2641
EarI CTCTTC 4 cut(s) 530, 1953, 2046, 2641
EciI GGCGGA 1 cut(s) 540
Ecl136II GAGCTC 2 cut(s) 540, 2848
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 3 cut(s) 542, 566, 2850
Eco31I GGTCTC 4 cut(s) 93, 386, 968, 1448
Eco32I GATATC 4 cut(s) 2391, 2556, 3057, 3499
Eco53kI GAGCTC 2 cut(s) 540, 2848
Eco57I CTGAAG 6 cut(s) 149, 501, 518, 1353, 1974, 2319
Eco81I CCTNAGG 1 cut(s) 3687
EcoICRI GAGCTC 2 cut(s) 540, 2848
EcoRI GAATTC 1 cut(s) 2263
EcoRII CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
EcoRV GATATC 4 cut(s) 2391, 2556, 3057, 3499
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
EcoT38I GRGCYC 3 cut(s) 542, 566, 2850
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 2 cut(s) 716, 3638
FaqI GGGAC 3 cut(s) 1330, 1591, 3607
FauI CCCGC 1 cut(s) 2378
FauNDI CATATG 2 cut(s) 2440, 2977
FbaI TGATCA 2 cut(s) 1275, 2980
FblI GTMKAC 4 cut(s) 493, 940, 2683, 3235
Fnu4HI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
FriOI GRGCYC 3 cut(s) 542, 566, 2850
Fsp4HI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
GlaI GCGC 1 cut(s) 3524
GluI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
GsaI CCCAGC 1 cut(s) 1968
HaeIII GGCC 4 cut(s) 149, 1539, 2513, 3404
HapII CCGG 1 cut(s) 862
HhaI GCGC 1 cut(s) 3525
Hin1I GRCGYC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 3523
HinP1I GCGC 1 cut(s) 3523
HincII GTYRAC 2 cut(s) 1321, 1797
HindII GTYRAC 2 cut(s) 1321, 1797
HindIII AAGCTT 2 cut(s) 2913, 3744
HpaI GTTAAC 1 cut(s) 1797
HpaII CCGG 1 cut(s) 862
HphI GGTGA 5 cut(s) 997, 2144, 2575, 3080, 3801
Hpy99I CGWCG 1 cut(s) 711
HpyCH4IV ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
HpyF10VI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
HpySE526I ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
Hsp92I GRCGYC 1 cut(s) 709
HspAI GCGC 1 cut(s) 3523
Ksp22I TGATCA 2 cut(s) 1275, 2980
KspAI GTTAAC 1 cut(s) 1797
LguI GCTCTTC 2 cut(s) 530, 2046
LmnI GCTCC 7 cut(s) 370, 511, 1361, 1846, 1992, 2067, 2357
Lsp1109I GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
MaeII ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
MbiI CCGCTC 1 cut(s) 74
MfeI CAATTG 1 cut(s) 1734
MflI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
MhlI GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
MlsI TGGCCA 1 cut(s) 1539
MluNI TGGCCA 1 cut(s) 1539
MlyI GAGTC 7 cut(s) 199, 1352, 1781, 2135, 2521, 2589, 3212
MmeI TCCRAC 6 cut(s) 217, 348, 429, 730, 1216, 1824
Mox20I TGGCCA 1 cut(s) 1539
Mph1103I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
MscI TGGCCA 1 cut(s) 1539
MslI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
Msp20I TGGCCA 1 cut(s) 1539
MspCI CTTAAG 1 cut(s) 1922
MspI CCGG 1 cut(s) 862
MspR9I CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 2 cut(s) 523, 1623
MvaI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
MvnI CGCG 2 cut(s) 70, 420
MwoI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
NdeI CATATG 2 cut(s) 2440, 2977
NlaIV GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
NmeAIII GCCGAG 1 cut(s) 3191
NmuCI GTSAC 4 cut(s) 1421, 2132, 2138, 2581
NsiI ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
NspI RCATGY 6 cut(s) 1117, 1258, 1723, 2350, 2490, 3491
NspV TTCGAA 1 cut(s) 3339
PaeI GCATGC 1 cut(s) 3491
PciI ACATGT 1 cut(s) 2346
PciSI GCTCTTC 2 cut(s) 530, 2046
PctI GAATGC 2 cut(s) 523, 1623
PflFI GACNNNGTC 1 cut(s) 1007
PflMI CCANNNNNTGG 2 cut(s) 746, 2537
PkrI GCNGC 8 cut(s) 91, 296, 637, 1383, 1681, 1769, 1930, 3694
PleI GAGTC 7 cut(s) 198, 1351, 1780, 2135, 2521, 2588, 3212
PpsI GAGTC 7 cut(s) 198, 1351, 1780, 2135, 2521, 2588, 3212
PscI ACATGT 1 cut(s) 2346
Psp124BI GAGCTC 2 cut(s) 542, 2850
Psp1406I AACGTT 1 cut(s) 2535
Psp6I CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
PspFI CCCAGC 1 cut(s) 1964
PspGI CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
PspN4I GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
PspPI GGNCC 2 cut(s) 2512, 3402
PstI CTGCAG 1 cut(s) 366
PsuI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
PsyI GACNNNGTC 1 cut(s) 1007
RsaI GTAC 7 cut(s) 759, 1248, 1407, 1565, 2364, 2408, 2569
RsaNI GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
RseI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
SacI GAGCTC 2 cut(s) 542, 2850
SapI GCTCTTC 2 cut(s) 530, 2046
SatI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
Sau96I GGNCC 2 cut(s) 2512, 3402
ScaI AGTACT 1 cut(s) 1248
SchI GAGTC 7 cut(s) 199, 1352, 1781, 2135, 2521, 2589, 3212
ScrFI CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
SduI GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
SfcI CTRYAG 1 cut(s) 362
SfuI TTCGAA 1 cut(s) 3339
SgrAI CRCCGGYG 1 cut(s) 861
SmiI ATTTAAAT 1 cut(s) 3016
SmiMI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
SmlI CTYRAG 8 cut(s) 428, 1449, 1816, 1922, 2033, 2370, 2910, 3637
SmoI CTYRAG 8 cut(s) 428, 1449, 1816, 1922, 2033, 2370, 2910, 3637
SpeI ACTAGT 1 cut(s) 625
SphI GCATGC 1 cut(s) 3491
SsiI CCGC 4 cut(s) 74, 418, 525, 2385
SspI AATATT 4 cut(s) 997, 2205, 2839, 3786
SstI GAGCTC 2 cut(s) 542, 2850
StyD4I CCNGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
StyI CCWWGG 1 cut(s) 558
SwaI ATTTAAAT 1 cut(s) 3016
TaiI ACGT 5 cut(s) 712, 2538, 2683, 3078, 3634
TaqI TCGA 7 cut(s) 1104, 1167, 2146, 3276, 3339, 3657, 3731
TatI WGTACW 3 cut(s) 1246, 1405, 1563
TseFI GTSAC 4 cut(s) 1421, 2132, 2138, 2581
TseI GCWGC 8 cut(s) 89, 294, 635, 1381, 1679, 1767, 1928, 3692
Tsp45I GTSAC 4 cut(s) 1421, 2132, 2138, 2581
TspGWI ACGGA 2 cut(s) 1348, 2561
Tth111I GACNNNGTC 1 cut(s) 1007
Van91I CCANNNNNTGG 2 cut(s) 746, 2537
Vha464I CTTAAG 1 cut(s) 1922
VneI GTGCAC 1 cut(s) 3189
XbaI TCTAGA 2 cut(s) 1489, 3214
XceI RCATGY 6 cut(s) 1117, 1258, 1723, 2350, 2490, 3491
XmiI GTMKAC 4 cut(s) 493, 940, 2683, 3235
ZraI GACGTC 1 cut(s) 710
ZrmI AGTACT 1 cut(s) 1248
Zsp2I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.