Rh2BG504800

Methylmalonate-semialdehyde dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000503
Physical Location & Seq
Forward (+)
71030069 .. 71033048
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG504800.1

Sequence Viewer

Length: 318 bp
ATGGATATTACTCTAGTTTATGATTGTACTCTCACACTACATTCTTTACTTCAAAAGGCTGGCTTGCACATATTTTCAAGAGCATCAGCTAAAGGGAAACGCGTTCAGTCAAATATGGGGGCTAAGAACCATGTAATTGTGATGCCTGATGCAAGTCCTGATGCCACTTTGAATGGTTTAGTTGCCGCTGGTTTTGGTGCTGCAGGACAAAGGTGCATGGCACTCAGCACAGTTGTTTTTGTTGGAGGTTCCAAGTCATGGGAGAACAAACTAGTGGAATGTGGCAAATATCTAAGTCAGTGCTGGAACAGAACCTGA

Protein Analysis

105

Amino Acids

11.24

Weight (kDa)

9.27

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 19 - 80 3.6e-13 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14170
fragaria_vesca FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11410 FvH4_2g11410
malus_domestica MD09G1234200.v1.1 MD15G1309700.v1.1
prunus_persica Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1
pyrus_communis pycom09g15250 pycom15g27380
rosa_chinensis RchiOBHm_Chr2g0110691 RchiOBHm_Chr2g0137621 RchiOBHm_Chr6g0270041 RchiOBHm_Chr6g0270061 RchiOBHm_Chr6g0270141
rosa_laevigata RLG00000013815 RLG00000013819 RLG00000017831 RLG00000019609
rosa_multiflora Rmu_co8442727.1_g000001 Rmu_sc0000389.1_g000042 Rmu_sc0000918.1_g000033 Rmu_sc0003043.1_g000019 Rmu_sc0004376.1_g000013
rosa_roxburghii Rroxscaffold_2G00133030 Rroxscaffold_5G00350410 Rroxscaffold_5G00350420 Rroxscaffold_5G00350430 Rroxscaffold_6G00398360 Rroxscaffold_7G00198140 Rroxscaffold_7G00198180
rosa_rugosa Rorug02G0168700 Rorug02G0168800 Rorug02G0168800 Rorug02G0338300 Rorug06G0052100 Rorug06G0052200 Rorug06G0052800 Rorug06G0052900
rosa_samantha Rh2AG221000 Rh2AG387100 Rh2AG387200 Rh2AG547100 Rh2BG231100 Rh2BG504800 Rh2CG223400 Rh2CG374500 Rh2DG227200 Rh2DG410100 Rh2DG516200 Rh5CG251300 Rh6AG171200 Rh6AG171700 Rh6BG175700 Rh6BG176100 Rh6CG170500 Rh6CG171200 Rh6CG216400 Rh6DG162700 Rh6DG163000
rosa_wichuraiana Rw2G017050 Rw6G014700 Rw6G014740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 258
AccII CGCG 1 cut(s) 102
AciI CCGC 1 cut(s) 186
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 258
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 3 cut(s) 53, 78, 172
AhlI ACTAGT 1 cut(s) 271
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 187
BcuI ACTAGT 1 cut(s) 271
BfaI CTAG 2 cut(s) 14, 272
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 2 cut(s) 186, 201
BlsI GCNGC 2 cut(s) 187, 202
BmiI GGNNCC 1 cut(s) 250
BmsI GCATC 4 cut(s) 92, 132, 139, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 258
BspACI CCGC 1 cut(s) 186
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 250
BspMAI CTGCAG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 61, 65
BstDEI CTNAG 3 cut(s) 123, 224, 293
BstFNI CGCG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 2 cut(s) 61, 65
CaiI CAGNNNCTG 1 cut(s) 315
Csp6I GTAC 1 cut(s) 27
CviAII CATG 3 cut(s) 131, 217, 258
CviJI RGCY 4 cut(s) 59, 63, 89, 122
CviKI_1 RGCY 4 cut(s) 59, 63, 89, 122
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 3 cut(s) 123, 224, 293
FaeI CATG 3 cut(s) 134, 220, 261
FaiI YATR 6 cut(s) 21, 71, 116, 132, 218, 259
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FatI CATG 3 cut(s) 130, 216, 257
Fnu4HI GCNGC 2 cut(s) 186, 201
Fsp4HI GCNGC 2 cut(s) 186, 201
FspBI CTAG 2 cut(s) 14, 272
GluI GCNGC 2 cut(s) 186, 201
Hin1II CATG 3 cut(s) 134, 220, 261
Hpy188III TCNNGA 2 cut(s) 78, 158
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4V TGCA 4 cut(s) 67, 152, 203, 216
HpyF3I CTNAG 3 cut(s) 123, 224, 293
Hsp92II CATG 3 cut(s) 134, 220, 261
LpnPI CCDG 6 cut(s) 45, 159, 171, 174, 189, 289
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 4 cut(s) 92, 132, 139, 151
MaeI CTAG 2 cut(s) 14, 272
MluCI AATT 1 cut(s) 135
MluI ACGCGT 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 188
MvnI CGCG 1 cut(s) 102
NlaIII CATG 3 cut(s) 134, 220, 261
NlaIV GGNNCC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 2 cut(s) 187, 202
PspN4I GGNNCC 1 cut(s) 250
PstI CTGCAG 1 cut(s) 205
PstNI CAGNNNCTG 1 cut(s) 315
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SatI GCNGC 2 cut(s) 186, 201
SetI ASST 4 cut(s) 91, 215, 250, 317
SfaNI GCATC 4 cut(s) 92, 132, 139, 151
SfcI CTRYAG 1 cut(s) 201
SpeI ACTAGT 1 cut(s) 271
Sse9I AATT 1 cut(s) 135
SsiI CCGC 1 cut(s) 186
SspMI CTAG 2 cut(s) 14, 272
TaaI ACNGT 1 cut(s) 232
TasI AATT 1 cut(s) 135
TatI WGTACW 1 cut(s) 26
TauI GCSGC 1 cut(s) 188
TscAI CASTG 1 cut(s) 305
TseI GCWGC 1 cut(s) 200
TspRI CASTG 1 cut(s) 305
Van91I CCANNNNNTGG 1 cut(s) 258
XspI CTAG 2 cut(s) 14, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.