Aldehyde dehydrogenase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Aldedh | PF00171 | 221 - 601 | 3.1e-114 | Aldehyde dehydrogenase family |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 254, 1982 |
| Acc36I | ACCTGC | 2 cut(s) | 1789, 2218 |
| AccB1I | GGYRCC | 1 cut(s) | 2188 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 59 |
| AccI | GTMKAC | 1 cut(s) | 1876 |
| AccII | CGCG | 1 cut(s) | 433 |
| AciI | CCGC | 4 cut(s) | 431, 570, 856, 1650 |
| AclWI | GGATC | 2 cut(s) | 508, 1562 |
| AcoI | YGGCCR | 1 cut(s) | 2163 |
| AcsI | RAATTY | 4 cut(s) | 241, 659, 1708, 1770 |
| AcuI | CTGAAG | 1 cut(s) | 827 |
| AdeI | CACNNNGTG | 1 cut(s) | 1049 |
| AfaI | GTAC | 4 cut(s) | 303, 1309, 1803, 2208 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 59, 602, 603, 1948, 2175 |
| AflII | CTTAAG | 2 cut(s) | 443, 1142 |
| AflIII | ACRYGT | 3 cut(s) | 348, 818, 1121 |
| AgeI | ACCGGT | 1 cut(s) | 1780 |
| AhlI | ACTAGT | 1 cut(s) | 1525 |
| AjiI | CACGTC | 1 cut(s) | 821 |
| AjnI | CCWGG | 1 cut(s) | 1162 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1758, 1790 |
| AleI | CACNNNNGTG | 1 cut(s) | 676 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1341, 1373, 1877, 1909 |
| Alw21I | GWGCWC | 2 cut(s) | 852, 936 |
| Alw26I | GTCTC | 2 cut(s) | 550, 2194 |
| AlwI | GGATC | 2 cut(s) | 508, 1562 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1804, 2128 |
| Ama87I | CYCGRG | 1 cut(s) | 1303 |
| AoxI | GGCC | 1 cut(s) | 2163 |
| ApeKI | GCWGC | 5 cut(s) | 761, 1184, 1454, 1952, 2036 |
| ApoI | RAATTY | 4 cut(s) | 241, 659, 1708, 1770 |
| AsiGI | ACCGGT | 1 cut(s) | 1780 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 542 |
| AspS9I | GGNCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| AsuC2I | CCSGG | 1 cut(s) | 1060 |
| AsuHPI | GGTGA | 5 cut(s) | 142, 589, 880, 1001, 1618 |
| AsuNHI | GCTAGC | 1 cut(s) | 1406 |
| AvaI | CYCGRG | 1 cut(s) | 1303 |
| AvaII | GGWCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| BalI | TGGCCA | 1 cut(s) | 2165 |
| BanI | GGYRCC | 1 cut(s) | 2188 |
| BanII | GRGCYC | 1 cut(s) | 852 |
| Bbv12I | GWGCWC | 2 cut(s) | 852, 936 |
| BbvI | GCAGC | 5 cut(s) | 748, 1196, 1441, 1939, 2023 |
| BccI | CCATC | 6 cut(s) | 143, 964, 979, 1208, 1397, 1664 |
| BceAI | ACGGC | 2 cut(s) | 613, 1455 |
| BciT130I | CCWGG | 1 cut(s) | 1164 |
| BcnI | CCSGG | 1 cut(s) | 1060 |
| BcoDI | GTCTC | 2 cut(s) | 550, 2194 |
| BcuI | ACTAGT | 1 cut(s) | 1525 |
| BfaI | CTAG | 6 cut(s) | 114, 1344, 1407, 1526, 1656, 1961 |
| BfmI | CTRYAG | 5 cut(s) | 473, 762, 980, 1455, 2214 |
| BfrI | CTTAAG | 2 cut(s) | 443, 1142 |
| BfuAI | ACCTGC | 2 cut(s) | 1789, 2218 |
| BglII | AGATCT | 1 cut(s) | 82 |
| BisI | GCNGC | 5 cut(s) | 762, 1185, 1455, 1953, 2037 |
| BlsI | GCNGC | 5 cut(s) | 763, 1186, 1456, 1954, 2038 |
| Bme1390I | CCNGG | 2 cut(s) | 1060, 1164 |
| Bme18I | GGWCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| BmeT110I | CYCGRG | 1 cut(s) | 1303 |
| BmgBI | CACGTC | 1 cut(s) | 821 |
| BmgT120I | GGNCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| BmiI | GGNNCC | 3 cut(s) | 639, 1041, 2190 |
| BmrFI | CCNGG | 2 cut(s) | 1060, 1164 |
| BmrI | ACTGGG | 3 cut(s) | 591, 1103, 1566 |
| BmsI | GCATC | 5 cut(s) | 367, 603, 1016, 1393, 1405 |
| BmtI | GCTAGC | 1 cut(s) | 1410 |
| BmuI | ACTGGG | 3 cut(s) | 591, 1103, 1566 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1584 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1194 |
| BpuEI | CTTGAG | 1 cut(s) | 437 |
| BpuMI | CCSGG | 1 cut(s) | 1060 |
| BsaI | GGTCTC | 1 cut(s) | 550 |
| BsaJI | CCNNGG | 4 cut(s) | 485, 937, 1234, 2174 |
| BsaWI | WCCGGW | 1 cut(s) | 1780 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1877, 1907 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 59, 602, 603, 1948, 2175 |
| Bse118I | RCCGGY | 1 cut(s) | 1780 |
| Bse1I | ACTGG | 4 cut(s) | 597, 1109, 1572, 1589 |
| Bse3DI | GCAATG | 2 cut(s) | 2031, 2115 |
| BseBI | CCWGG | 1 cut(s) | 1164 |
| BseDI | CCNNGG | 4 cut(s) | 485, 937, 1234, 2174 |
| BseGI | GGATG | 6 cut(s) | 87, 358, 852, 975, 1107, 1420 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 59, 602, 603, 1948, 2175 |
| BseMI | GCAATG | 2 cut(s) | 2031, 2115 |
| BseMII | CTCAG | 2 cut(s) | 372, 912 |
| BseNI | ACTGG | 4 cut(s) | 597, 1109, 1572, 1589 |
| BseXI | GCAGC | 5 cut(s) | 748, 1196, 1441, 1939, 2023 |
| BseYI | CCCAGC | 1 cut(s) | 2066 |
| BsgI | GTGCAG | 1 cut(s) | 1596 |
| Bsh1236I | CGCG | 1 cut(s) | 433 |
| BshFI | GGCC | 1 cut(s) | 2165 |
| BshNI | GGYRCC | 1 cut(s) | 2188 |
| BshTI | ACCGGT | 1 cut(s) | 1780 |
| BsiHKAI | GWGCWC | 2 cut(s) | 852, 936 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1303 |
| BsiSI | CCGG | 2 cut(s) | 1059, 1781 |
| BslFI | GGGAC | 1 cut(s) | 246 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 59, 602, 603, 1948, 2175 |
| BsmAI | GTCTC | 2 cut(s) | 550, 2194 |
| BsmFI | GGGAC | 1 cut(s) | 246 |
| BsmI | GAATGC | 2 cut(s) | 1323, 1432 |
| BsnI | GGCC | 1 cut(s) | 2165 |
| Bso31I | GGTCTC | 1 cut(s) | 550 |
| BsoBI | CYCGRG | 1 cut(s) | 1303 |
| Bsp1286I | GDGCHC | 2 cut(s) | 852, 936 |
| Bsp143I | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| Bsp19I | CCATGG | 2 cut(s) | 1234, 2174 |
| BspACI | CCGC | 4 cut(s) | 431, 570, 856, 1650 |
| BspANI | GGCC | 1 cut(s) | 2165 |
| BspCNI | CTCAG | 2 cut(s) | 373, 913 |
| BspFNI | CGCG | 1 cut(s) | 433 |
| BspHI | TCATGA | 2 cut(s) | 331, 2196 |
| BspLI | GGNNCC | 3 cut(s) | 639, 1041, 2190 |
| BspMAI | CTGCAG | 3 cut(s) | 477, 766, 1459 |
| BspMI | ACCTGC | 2 cut(s) | 1789, 2218 |
| BspOI | GCTAGC | 1 cut(s) | 1410 |
| BspPI | GGATC | 2 cut(s) | 508, 1562 |
| BspT107I | GGYRCC | 1 cut(s) | 2188 |
| BspTI | CTTAAG | 2 cut(s) | 443, 1142 |
| BspTNI | GGTCTC | 1 cut(s) | 550 |
| BsrDI | GCAATG | 2 cut(s) | 2031, 2115 |
| BsrFI | RCCGGY | 1 cut(s) | 1780 |
| BsrI | ACTGG | 4 cut(s) | 597, 1109, 1572, 1589 |
| BssAI | RCCGGY | 1 cut(s) | 1780 |
| BssECI | CCNNGG | 4 cut(s) | 485, 937, 1234, 2174 |
| BssMI | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| BssNAI | GTATAC | 1 cut(s) | 1877 |
| BssT1I | CCWWGG | 3 cut(s) | 485, 1234, 2174 |
| Bst1107I | GTATAC | 1 cut(s) | 1877 |
| Bst2UI | CCWGG | 1 cut(s) | 1164 |
| Bst4CI | ACNGT | 3 cut(s) | 260, 1806, 2218 |
| Bst6I | CTCTTC | 2 cut(s) | 381, 744 |
| BstAFI | CTTAAG | 2 cut(s) | 443, 1142 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1190 |
| BstC8I | GCNNGC | 4 cut(s) | 783, 1408, 1796, 1957 |
| BstDEI | CTNAG | 4 cut(s) | 381, 892, 921, 1194 |
| BstDSI | CCRYGG | 3 cut(s) | 937, 1234, 2174 |
| BstEII | GGTNACC | 2 cut(s) | 130, 1917 |
| BstF5I | GGATG | 6 cut(s) | 87, 358, 852, 975, 1107, 1420 |
| BstFNI | CGCG | 1 cut(s) | 433 |
| BstKTI | GATC | 5 cut(s) | 76, 85, 516, 1570, 1974 |
| BstMAI | GTCTC | 2 cut(s) | 550, 2194 |
| BstMBI | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 403, 782, 847, 1190, 1199 |
| BstNI | CCWGG | 1 cut(s) | 1164 |
| BstNSI | RCATGY | 5 cut(s) | 352, 962, 1125, 1331, 1798 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1584 |
| BstPI | GGTNACC | 2 cut(s) | 130, 1917 |
| BstSCI | CCNGG | 2 cut(s) | 1058, 1162 |
| BstSFI | CTRYAG | 5 cut(s) | 473, 762, 980, 1455, 2214 |
| BstUI | CGCG | 1 cut(s) | 433 |
| BstV1I | GCAGC | 5 cut(s) | 748, 1196, 1441, 1939, 2023 |
| BstX2I | RGATCY | 3 cut(s) | 82, 513, 1567 |
| BstYI | RGATCY | 3 cut(s) | 82, 513, 1567 |
| BstZ17I | GTATAC | 1 cut(s) | 1877 |
| BsuRI | GGCC | 1 cut(s) | 2165 |
| BtgI | CCRYGG | 3 cut(s) | 937, 1234, 2174 |
| BtrI | CACGTC | 1 cut(s) | 821 |
| BtsCI | GGATG | 6 cut(s) | 87, 358, 852, 975, 1107, 1420 |
| BtsI | GCAGTG | 1 cut(s) | 1645 |
| BtsIMutI | CAGTG | 3 cut(s) | 604, 1579, 1645 |
| BveI | ACCTGC | 2 cut(s) | 1789, 2218 |
| Cac8I | GCNNGC | 4 cut(s) | 783, 1408, 1796, 1957 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1804, 2128 |
| CciI | TCATGA | 2 cut(s) | 331, 2196 |
| Cfr10I | RCCGGY | 1 cut(s) | 1780 |
| Cfr13I | GGNCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| CseI | GACGC | 1 cut(s) | 422 |
| Csp6I | GTAC | 4 cut(s) | 302, 1308, 1802, 2207 |
| CspAI | ACCGGT | 1 cut(s) | 1780 |
| CviQI | GTAC | 4 cut(s) | 302, 1308, 1802, 2207 |
| DdeI | CTNAG | 4 cut(s) | 381, 892, 921, 1194 |
| DpnI | GATC | 5 cut(s) | 75, 84, 515, 1569, 1973 |
| DpnII | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| DraI | TTTAAA | 1 cut(s) | 2107 |
| DraIII | CACNNNGTG | 1 cut(s) | 1049 |
| EaeI | YGGCCR | 1 cut(s) | 2163 |
| Eam1104I | CTCTTC | 2 cut(s) | 381, 744 |
| EarI | CTCTTC | 2 cut(s) | 381, 744 |
| Ecl136II | GAGCTC | 1 cut(s) | 850 |
| Eco130I | CCWWGG | 3 cut(s) | 485, 1234, 2174 |
| Eco24I | GRGCYC | 1 cut(s) | 852 |
| Eco31I | GGTCTC | 1 cut(s) | 550 |
| Eco47I | GGWCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| Eco53kI | GAGCTC | 1 cut(s) | 850 |
| Eco57I | CTGAAG | 1 cut(s) | 827 |
| Eco88I | CYCGRG | 1 cut(s) | 1303 |
| Eco91I | GGTNACC | 2 cut(s) | 130, 1917 |
| EcoICRI | GAGCTC | 1 cut(s) | 850 |
| EcoO65I | GGTNACC | 2 cut(s) | 130, 1917 |
| EcoRII | CCWGG | 1 cut(s) | 1162 |
| EcoT14I | CCWWGG | 3 cut(s) | 485, 1234, 2174 |
| EcoT22I | ATGCAT | 1 cut(s) | 1009 |
| EcoT38I | GRGCYC | 1 cut(s) | 852 |
| ErhI | CCWWGG | 3 cut(s) | 485, 1234, 2174 |
| FaqI | GGGAC | 1 cut(s) | 246 |
| FauNDI | CATATG | 1 cut(s) | 1619 |
| FblI | GTMKAC | 1 cut(s) | 1876 |
| Fnu4HI | GCNGC | 5 cut(s) | 762, 1185, 1455, 1953, 2037 |
| FokI | GGATG | 6 cut(s) | 74, 345, 839, 982, 1114, 1427 |
| FriOI | GRGCYC | 1 cut(s) | 852 |
| Fsp4HI | GCNGC | 5 cut(s) | 762, 1185, 1455, 1953, 2037 |
| FspBI | CTAG | 6 cut(s) | 114, 1344, 1407, 1526, 1656, 1961 |
| GluI | GCNGC | 5 cut(s) | 762, 1185, 1455, 1953, 2037 |
| GsaI | CCCAGC | 1 cut(s) | 2070 |
| HaeIII | GGCC | 1 cut(s) | 2165 |
| HapII | CCGG | 2 cut(s) | 1059, 1781 |
| HgaI | GACGC | 1 cut(s) | 422 |
| HincII | GTYRAC | 1 cut(s) | 480 |
| HindII | GTYRAC | 1 cut(s) | 480 |
| HindIII | AAGCTT | 2 cut(s) | 783, 839 |
| HinfI | GANTC | 5 cut(s) | 463, 668, 828, 1090, 2202 |
| HpaII | CCGG | 2 cut(s) | 1059, 1781 |
| HphI | GGTGA | 5 cut(s) | 142, 589, 880, 1001, 1618 |
| Hpy166II | GTNNAC | 4 cut(s) | 480, 1523, 1809, 1877 |
| Hpy188I | TCNGA | 2 cut(s) | 355, 535 |
| Hpy8I | GTNNAC | 4 cut(s) | 480, 1523, 1809, 1877 |
| Hpy99I | CGWCG | 1 cut(s) | 375 |
| HpyAV | CCTTC | 7 cut(s) | 229, 628, 801, 907, 1081, 1603, 2162 |
| HpyCH4III | ACNGT | 3 cut(s) | 260, 1806, 2218 |
| HpyCH4IV | ACGT | 6 cut(s) | 693, 820, 908, 1534, 1581, 2140 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 403, 782, 847, 1190, 1199 |
| HpyF3I | CTNAG | 4 cut(s) | 381, 892, 921, 1194 |
| HpySE526I | ACGT | 6 cut(s) | 693, 820, 908, 1534, 1581, 2140 |
| Kzo9I | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| LmnI | GCTCC | 5 cut(s) | 322, 598, 847, 939, 2006 |
| Lsp1109I | GCAGC | 5 cut(s) | 748, 1196, 1441, 1939, 2023 |
| LweI | GCATC | 5 cut(s) | 367, 603, 1016, 1393, 1405 |
| MaeI | CTAG | 6 cut(s) | 114, 1344, 1407, 1526, 1656, 1961 |
| MaeII | ACGT | 6 cut(s) | 693, 820, 908, 1534, 1581, 2140 |
| MaeIII | GTNAC | 6 cut(s) | 130, 620, 1117, 1435, 1738, 1917 |
| MalI | GATC | 5 cut(s) | 75, 84, 515, 1569, 1973 |
| MboI | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| MboII | GAAGA | 8 cut(s) | 92, 299, 398, 761, 811, 1778, 1778, 1976 |
| MfeI | CAATTG | 1 cut(s) | 2181 |
| MflI | RGATCY | 3 cut(s) | 82, 513, 1567 |
| MhlI | GDGCHC | 2 cut(s) | 852, 936 |
| MlsI | TGGCCA | 1 cut(s) | 2165 |
| MluNI | TGGCCA | 1 cut(s) | 2165 |
| MlyI | GAGTC | 1 cut(s) | 2196 |
| MmeI | TCCRAC | 1 cut(s) | 1477 |
| Mox20I | TGGCCA | 1 cut(s) | 2165 |
| Mph1103I | ATGCAT | 1 cut(s) | 1009 |
| MroXI | GAANNNNTTC | 1 cut(s) | 542 |
| MscI | TGGCCA | 1 cut(s) | 2165 |
| MslI | CAYNNNNRTG | 7 cut(s) | 330, 676, 963, 1326, 1394, 1516, 1862 |
| Msp20I | TGGCCA | 1 cut(s) | 2165 |
| MspA1I | CMGCKG | 2 cut(s) | 1313, 1487 |
| MspCI | CTTAAG | 2 cut(s) | 443, 1142 |
| MspI | CCGG | 2 cut(s) | 1059, 1781 |
| MspR9I | CCNGG | 2 cut(s) | 1060, 1164 |
| MssI | GTTTAAAC | 1 cut(s) | 2107 |
| MunI | CAATTG | 1 cut(s) | 2181 |
| Mva1269I | GAATGC | 2 cut(s) | 1323, 1432 |
| MvaI | CCWGG | 1 cut(s) | 1164 |
| MvnI | CGCG | 1 cut(s) | 433 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 403, 782, 847, 1190, 1199 |
| NciI | CCSGG | 1 cut(s) | 1060 |
| NcoI | CCATGG | 2 cut(s) | 1234, 2174 |
| NdeI | CATATG | 1 cut(s) | 1619 |
| NdeII | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| NheI | GCTAGC | 1 cut(s) | 1406 |
| NlaIV | GGNNCC | 3 cut(s) | 639, 1041, 2190 |
| NmuCI | GTSAC | 2 cut(s) | 130, 1435 |
| NsiI | ATGCAT | 1 cut(s) | 1009 |
| NspI | RCATGY | 5 cut(s) | 352, 962, 1125, 1331, 1798 |
| OliI | CACNNNNGTG | 1 cut(s) | 676 |
| PaeI | GCATGC | 1 cut(s) | 1798 |
| PaeR7I | CTCGAG | 1 cut(s) | 1303 |
| PagI | TCATGA | 2 cut(s) | 331, 2196 |
| PciI | ACATGT | 2 cut(s) | 348, 1121 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 202 |
| PctI | GAATGC | 2 cut(s) | 1323, 1432 |
| PdmI | GAANNNNTTC | 1 cut(s) | 542 |
| PfeI | GAWTC | 4 cut(s) | 463, 668, 828, 1090 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 59 |
| PfoI | TCCNGGA | 1 cut(s) | 1162 |
| PinAI | ACCGGT | 1 cut(s) | 1780 |
| PkrI | GCNGC | 5 cut(s) | 763, 1186, 1456, 1954, 2038 |
| PleI | GAGTC | 1 cut(s) | 2196 |
| PmeI | GTTTAAAC | 1 cut(s) | 2107 |
| PpsI | GAGTC | 1 cut(s) | 2196 |
| PscI | ACATGT | 2 cut(s) | 348, 1121 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1584 |
| PsiI | TTATAA | 2 cut(s) | 254, 1982 |
| Psp124BI | GAGCTC | 1 cut(s) | 852 |
| Psp6I | CCWGG | 1 cut(s) | 1162 |
| PspEI | GGTNACC | 2 cut(s) | 130, 1917 |
| PspFI | CCCAGC | 1 cut(s) | 2066 |
| PspGI | CCWGG | 1 cut(s) | 1162 |
| PspN4I | GGNNCC | 3 cut(s) | 639, 1041, 2190 |
| PspPI | GGNCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| PspXI | VCTCGAGB | 1 cut(s) | 1303 |
| PstI | CTGCAG | 3 cut(s) | 477, 766, 1459 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1804, 2128 |
| PsuI | RGATCY | 3 cut(s) | 82, 513, 1567 |
| PvuII | CAGCTG | 2 cut(s) | 1313, 1487 |
| RsaI | GTAC | 4 cut(s) | 303, 1309, 1803, 2208 |
| RsaNI | GTAC | 4 cut(s) | 302, 1308, 1802, 2207 |
| RseI | CAYNNNNRTG | 7 cut(s) | 330, 676, 963, 1326, 1394, 1516, 1862 |
| SacI | GAGCTC | 1 cut(s) | 852 |
| SatI | GCNGC | 5 cut(s) | 762, 1185, 1455, 1953, 2037 |
| Sau3AI | GATC | 5 cut(s) | 73, 82, 513, 1567, 1971 |
| Sau96I | GGNCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| SchI | GAGTC | 1 cut(s) | 2196 |
| ScrFI | CCNGG | 2 cut(s) | 1060, 1164 |
| SduI | GDGCHC | 2 cut(s) | 852, 936 |
| SfaNI | GCATC | 5 cut(s) | 367, 603, 1016, 1393, 1405 |
| SfcI | CTRYAG | 5 cut(s) | 473, 762, 980, 1455, 2214 |
| Sfr274I | CTCGAG | 1 cut(s) | 1303 |
| SinI | GGWCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| SlaI | CTCGAG | 1 cut(s) | 1303 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 330, 676, 963, 1326, 1394, 1516, 1862 |
| SmlI | CTYRAG | 4 cut(s) | 443, 452, 1142, 1303 |
| SmoI | CTYRAG | 4 cut(s) | 443, 452, 1142, 1303 |
| SpeI | ACTAGT | 1 cut(s) | 1525 |
| SphI | GCATGC | 1 cut(s) | 1798 |
| SsiI | CCGC | 4 cut(s) | 431, 570, 856, 1650 |
| SspMI | CTAG | 6 cut(s) | 114, 1344, 1407, 1526, 1656, 1961 |
| SstI | GAGCTC | 1 cut(s) | 852 |
| StyD4I | CCNGG | 2 cut(s) | 1058, 1162 |
| StyI | CCWWGG | 3 cut(s) | 485, 1234, 2174 |
| TaaI | ACNGT | 3 cut(s) | 260, 1806, 2218 |
| TaiI | ACGT | 6 cut(s) | 696, 823, 911, 1537, 1584, 2143 |
| TaqI | TCGA | 4 cut(s) | 954, 1304, 1690, 2155 |
| TaqII | GACCGA | 1 cut(s) | 428 |
| TatI | WGTACW | 1 cut(s) | 1307 |
| TfiI | GAWTC | 4 cut(s) | 463, 668, 828, 1090 |
| TscAI | CASTG | 3 cut(s) | 604, 1579, 1645 |
| TseFI | GTSAC | 2 cut(s) | 130, 1435 |
| TseI | GCWGC | 5 cut(s) | 761, 1184, 1454, 1952, 2036 |
| Tsp45I | GTSAC | 2 cut(s) | 130, 1435 |
| TspDTI | ATGAA | 9 cut(s) | 231, 348, 476, 637, 762, 783, 896, 2070, 2104 |
| TspGWI | ACGGA | 2 cut(s) | 506, 926 |
| TspRI | CASTG | 3 cut(s) | 604, 1579, 1645 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 59 |
| Vha464I | CTTAAG | 2 cut(s) | 443, 1142 |
| VpaK11BI | GGWCC | 4 cut(s) | 567, 1586, 1718, 1778 |
| XapI | RAATTY | 4 cut(s) | 241, 659, 1708, 1770 |
| XceI | RCATGY | 5 cut(s) | 352, 962, 1125, 1331, 1798 |
| XhoI | CTCGAG | 1 cut(s) | 1303 |
| XmiI | GTMKAC | 1 cut(s) | 1876 |
| XmnI | GAANNNNTTC | 1 cut(s) | 542 |
| XspI | CTAG | 6 cut(s) | 114, 1344, 1407, 1526, 1656, 1961 |
| Zsp2I | ATGCAT | 1 cut(s) | 1009 |