RchiOBHm_Chr6g0270061

Methylmalonate-semialdehyde dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000503
Physical Location & Seq
Forward (+)
27757914 .. 27758311
398 bp
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UTR
Exon/CDS
Intron
PRQ24227

Sequence Viewer

Length: 288 bp
ATGAAATCTTACGGGATTCACTCACAGGCCAAGGAGCGCATATGCAATCTAATCCAATCTGGTGTTGAAAGTGGTGCCAGGCTATTGTTGGATGGGCGAGATATAGAGGTCCCTGCAGGTTATGAGCATGGCAATTTTATTGGCCCTACTATCTCAGCAAATGTTACAGCTGACATGGAGTGCTACAAGGTATTTTTGGCATTTTTCTTTTCCTTAAATTTTCCATTTTATTTTCTGCTTGGTGGATCAATCGTAGTTTCTAGAGAGCACTTGGTAGATGATGAATGA

Protein Analysis

95

Amino Acids

10.56

Weight (kDa)

5.02

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 6 - 63 4.1e-09 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14170
fragaria_vesca FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11410 FvH4_2g11410
malus_domestica MD09G1234200.v1.1 MD15G1309700.v1.1
prunus_persica Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1
pyrus_communis pycom09g15250 pycom15g27380
rosa_chinensis RchiOBHm_Chr2g0110691 RchiOBHm_Chr2g0137621 RchiOBHm_Chr6g0270041 RchiOBHm_Chr6g0270061 RchiOBHm_Chr6g0270141
rosa_laevigata RLG00000013815 RLG00000013819 RLG00000017831 RLG00000019609
rosa_multiflora Rmu_co8442727.1_g000001 Rmu_sc0000389.1_g000042 Rmu_sc0000918.1_g000033 Rmu_sc0003043.1_g000019 Rmu_sc0004376.1_g000013
rosa_roxburghii Rroxscaffold_2G00133030 Rroxscaffold_5G00350410 Rroxscaffold_5G00350420 Rroxscaffold_5G00350430 Rroxscaffold_6G00398360 Rroxscaffold_7G00198140 Rroxscaffold_7G00198180
rosa_rugosa Rorug02G0168700 Rorug02G0168800 Rorug02G0168800 Rorug02G0338300 Rorug06G0052100 Rorug06G0052200 Rorug06G0052800 Rorug06G0052900
rosa_samantha Rh2AG221000 Rh2AG387100 Rh2AG387200 Rh2AG547100 Rh2BG231100 Rh2BG504800 Rh2CG223400 Rh2CG374500 Rh2DG227200 Rh2DG410100 Rh2DG516200 Rh5CG251300 Rh6AG171200 Rh6AG171700 Rh6BG175700 Rh6BG176100 Rh6CG170500 Rh6CG171200 Rh6CG216400 Rh6DG162700 Rh6DG163000
rosa_wichuraiana Rw2G017050 Rw6G014700 Rw6G014740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 74
AclWI GGATC 1 cut(s) 253
AcsI RAATTY 1 cut(s) 217
AgsI TTSAA 1 cut(s) 68
AjnI CCWGG 1 cut(s) 77
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw21I GWGCWC 1 cut(s) 270
AlwI GGATC 1 cut(s) 253
AoxI GGCC 2 cut(s) 27, 142
ApoI RAATTY 1 cut(s) 217
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 109, 143
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 270
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 79
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 143
BmiI GGNNCC 2 cut(s) 76, 111
BmrFI CCNGG 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 168
BshFI GGCC 2 cut(s) 29, 144
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 270
BslFI GGGAC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 29, 144
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 245
BspANI GGCC 2 cut(s) 29, 144
BspCNI CTCAG 1 cut(s) 167
BspLI GGNNCC 2 cut(s) 76, 111
BspMAI CTGCAG 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 97
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 1 cut(s) 248
BstMBI GATC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 114
BsuRI GGCC 2 cut(s) 29, 144
BtsCI GGATG 1 cut(s) 97
BveI ACCTGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 109, 143
CviAII CATG 2 cut(s) 128, 175
CviJI RGCY 4 cut(s) 29, 82, 144, 170
CviKI_1 RGCY 4 cut(s) 29, 82, 144, 170
DdeI CTNAG 1 cut(s) 154
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 131, 178
FaiI YATR 6 cut(s) 41, 43, 104, 123, 129, 176
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 127, 174
FauNDI CATATG 1 cut(s) 41
FokI GGATG 1 cut(s) 104
FspBI CTAG 1 cut(s) 261
GlaI GCGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 29, 144
HhaI GCGC 1 cut(s) 39
Hin1II CATG 2 cut(s) 131, 178
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 261
HpyCH4V TGCA 2 cut(s) 45, 116
HpyF3I CTNAG 1 cut(s) 154
Hsp92II CATG 2 cut(s) 131, 178
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 6 cut(s) 11, 45, 64, 91, 102, 126
MaeI CTAG 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 163
MalI GATC 1 cut(s) 247
MboI GATC 1 cut(s) 245
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 2 cut(s) 133, 217
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 1 cut(s) 100
MseI TTAA 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 41
NdeII GATC 1 cut(s) 245
NlaIII CATG 2 cut(s) 131, 178
NlaIV GGNNCC 2 cut(s) 76, 111
PfeI GAWTC 1 cut(s) 16
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 76, 111
PspPI GGNCC 2 cut(s) 109, 143
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 170
SaqAI TTAA 1 cut(s) 215
Sau3AI GATC 1 cut(s) 245
Sau96I GGNCC 2 cut(s) 109, 143
SbfI CCTGCAGG 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 79
SdaI CCTGCAGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 270
SetI ASST 4 cut(s) 111, 121, 172, 192
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 109
Sse8387I CCTGCAGG 1 cut(s) 118
Sse9I AATT 2 cut(s) 133, 217
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 30
TasI AATT 2 cut(s) 133, 217
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 217
XbaI TCTAGA 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.