Aldehyde dehydrogenase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 614, 1350, 2610 |
| AarI | CACCTGC | 1 cut(s) | 2153 |
| Acc36I | ACCTGC | 2 cut(s) | 2153, 2443 |
| AccI | GTMKAC | 2 cut(s) | 2427, 2957 |
| AciI | CCGC | 3 cut(s) | 44, 174, 418 |
| AclWI | GGATC | 7 cut(s) | 695, 907, 918, 1102, 2003, 2016, 2419 |
| AcoI | YGGCCR | 2 cut(s) | 489, 2831 |
| AcsI | RAATTY | 4 cut(s) | 113, 241, 1290, 2744 |
| AcuI | CTGAAG | 1 cut(s) | 491 |
| AcvI | CACGTG | 2 cut(s) | 434, 1787 |
| AdeI | CACNNNGTG | 1 cut(s) | 1898 |
| AfaI | GTAC | 4 cut(s) | 29, 1155, 1581, 1654 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 606, 896, 2188 |
| AflIII | ACRYGT | 2 cut(s) | 931, 1970 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1110 |
| AjnI | CCWGG | 4 cut(s) | 534, 895, 2011, 2769 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 744, 776, 2607, 2639 |
| Alw21I | GWGCWC | 3 cut(s) | 769, 2321, 2828 |
| Alw26I | GTCTC | 3 cut(s) | 69, 1704, 1786 |
| Alw44I | GTGCAC | 2 cut(s) | 765, 2317 |
| AlwI | GGATC | 7 cut(s) | 695, 907, 918, 1102, 2003, 2016, 2419 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1271 |
| Ama87I | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| AoxI | GGCC | 6 cut(s) | 201, 489, 745, 2626, 2767, 2831 |
| ApaLI | GTGCAC | 2 cut(s) | 765, 2317 |
| ApoI | RAATTY | 4 cut(s) | 113, 241, 1290, 2744 |
| ArsI | GACNNNNNNTTYG | 3 cut(s) | 28, 191, 223 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 924 |
| AspLEI | GCGC | 2 cut(s) | 414, 1473 |
| AspS9I | GGNCC | 5 cut(s) | 841, 2435, 2567, 2627, 2767 |
| AsuC2I | CCSGG | 2 cut(s) | 2541, 2830 |
| AsuHPI | GGTGA | 3 cut(s) | 710, 1151, 2858 |
| AvaI | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| AvaII | GGWCC | 3 cut(s) | 841, 2435, 2567 |
| BaeGI | GKGCMC | 2 cut(s) | 769, 2321 |
| BalI | TGGCCA | 1 cut(s) | 491 |
| BamHI | GGATCC | 1 cut(s) | 2008 |
| BanII | GRGCYC | 1 cut(s) | 2828 |
| BbrPI | CACGTG | 2 cut(s) | 434, 1787 |
| BbsI | GAAGAC | 5 cut(s) | 201, 497, 813, 2373, 2903 |
| Bbv12I | GWGCWC | 3 cut(s) | 769, 2321, 2828 |
| BbvCI | CCTCAGC | 1 cut(s) | 477 |
| BceAI | ACGGC | 1 cut(s) | 188 |
| BciT130I | CCWGG | 4 cut(s) | 536, 897, 2013, 2771 |
| BcnI | CCSGG | 2 cut(s) | 2541, 2830 |
| BcoDI | GTCTC | 3 cut(s) | 69, 1704, 1786 |
| BfaI | CTAG | 3 cut(s) | 540, 1793, 2282 |
| BfmI | CTRYAG | 8 cut(s) | 471, 807, 1063, 1611, 1829, 1876, 1962, 2304 |
| BfoI | RGCGCY | 1 cut(s) | 415 |
| BfuAI | ACCTGC | 2 cut(s) | 2153, 2443 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2775 |
| BglII | AGATCT | 1 cut(s) | 1321 |
| BlpI | GCTNAGC | 1 cut(s) | 561 |
| Bme1390I | CCNGG | 6 cut(s) | 536, 897, 2013, 2541, 2771, 2830 |
| Bme18I | GGWCC | 3 cut(s) | 841, 2435, 2567 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1110 |
| BmeT110I | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| BmgT120I | GGNCC | 5 cut(s) | 841, 2435, 2567, 2627, 2767 |
| BmiI | GGNNCC | 5 cut(s) | 1174, 1890, 2010, 2538, 2569 |
| BmrFI | CCNGG | 6 cut(s) | 536, 897, 2013, 2541, 2771, 2830 |
| BmrI | ACTGGG | 1 cut(s) | 2624 |
| BmuI | ACTGGG | 1 cut(s) | 2624 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2433 |
| BpiI | GAAGAC | 5 cut(s) | 201, 497, 813, 2373, 2903 |
| Bpu10I | CCTNAGC | 2 cut(s) | 477, 2193 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 561 |
| BpuEI | CTTGAG | 3 cut(s) | 866, 1560, 2381 |
| BpuMI | CCSGG | 2 cut(s) | 2541, 2830 |
| BsaAI | YACGTR | 2 cut(s) | 434, 1787 |
| BsaJI | CCNNGG | 3 cut(s) | 1006, 1372, 2259 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 606, 896, 2188 |
| Bse118I | RCCGGY | 2 cut(s) | 414, 2843 |
| Bse1I | ACTGG | 5 cut(s) | 141, 825, 2438, 2514, 2630 |
| Bse3DI | GCAATG | 5 cut(s) | 492, 685, 2049, 2707, 2801 |
| BseBI | CCWGG | 4 cut(s) | 536, 897, 2013, 2771 |
| BseDI | CCNNGG | 3 cut(s) | 1006, 1372, 2259 |
| BseGI | GGATG | 6 cut(s) | 370, 462, 843, 1210, 1771, 1956 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 606, 896, 2188 |
| BseMI | GCAATG | 5 cut(s) | 492, 685, 2049, 2707, 2801 |
| BseMII | CTCAG | 7 cut(s) | 468, 552, 621, 681, 773, 2745, 2808 |
| BseNI | ACTGG | 5 cut(s) | 141, 825, 2438, 2514, 2630 |
| BseRI | GAGGAG | 3 cut(s) | 649, 1035, 1613 |
| BseSI | GKGCMC | 2 cut(s) | 769, 2321 |
| BseYI | CCCAGC | 1 cut(s) | 1907 |
| BsgI | GTGCAG | 2 cut(s) | 456, 685 |
| Bsh1285I | CGRYCG | 1 cut(s) | 34 |
| BshFI | GGCC | 6 cut(s) | 203, 491, 747, 2628, 2769, 2833 |
| BsiEI | CGRYCG | 1 cut(s) | 34 |
| BsiHKAI | GWGCWC | 3 cut(s) | 769, 2321, 2828 |
| BsiHKCI | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| BsiSI | CCGG | 5 cut(s) | 415, 2409, 2541, 2830, 2844 |
| BslFI | GGGAC | 2 cut(s) | 953, 2553 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 606, 896, 2188 |
| BsmAI | GTCTC | 3 cut(s) | 69, 1704, 1786 |
| BsmFI | GGGAC | 2 cut(s) | 953, 2553 |
| BsmI | GAATGC | 1 cut(s) | 2281 |
| BsnI | GGCC | 6 cut(s) | 203, 491, 747, 2628, 2769, 2833 |
| BsoBI | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| Bsp1286I | GDGCHC | 3 cut(s) | 769, 2321, 2828 |
| Bsp1407I | TGTACA | 1 cut(s) | 1652 |
| Bsp143I | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 561 |
| Bsp19I | CCATGG | 1 cut(s) | 2259 |
| BspACI | CCGC | 3 cut(s) | 44, 174, 418 |
| BspANI | GGCC | 6 cut(s) | 203, 491, 747, 2628, 2769, 2833 |
| BspCNI | CTCAG | 7 cut(s) | 469, 553, 622, 682, 774, 2746, 2807 |
| BspHI | TCATGA | 1 cut(s) | 181 |
| BspLI | GGNNCC | 5 cut(s) | 1174, 1890, 2010, 2538, 2569 |
| BspMAI | CTGCAG | 6 cut(s) | 475, 811, 1615, 1833, 1966, 2308 |
| BspMI | ACCTGC | 2 cut(s) | 2153, 2443 |
| BspPI | GGATC | 7 cut(s) | 695, 907, 918, 1102, 2003, 2016, 2419 |
| BspQI | GCTCTTC | 1 cut(s) | 642 |
| BsrDI | GCAATG | 5 cut(s) | 492, 685, 2049, 2707, 2801 |
| BsrFI | RCCGGY | 2 cut(s) | 414, 2843 |
| BsrGI | TGTACA | 1 cut(s) | 1652 |
| BsrI | ACTGG | 5 cut(s) | 141, 825, 2438, 2514, 2630 |
| BssAI | RCCGGY | 2 cut(s) | 414, 2843 |
| BssECI | CCNNGG | 3 cut(s) | 1006, 1372, 2259 |
| BssMI | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| BssT1I | CCWWGG | 3 cut(s) | 1006, 1372, 2259 |
| Bst2UI | CCWGG | 4 cut(s) | 536, 897, 2013, 2771 |
| Bst4CI | ACNGT | 8 cut(s) | 32, 249, 1034, 1162, 1524, 2335, 2902, 2933 |
| Bst6I | CTCTTC | 2 cut(s) | 512, 642 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 299, 1619, 1631, 2165 |
| BstAUI | TGTACA | 1 cut(s) | 1652 |
| BstBAI | YACGTR | 2 cut(s) | 434, 1787 |
| BstC8I | GCNNGC | 9 cut(s) | 416, 668, 796, 2031, 2053, 2057, 2293, 2385, 2450 |
| BstDSI | CCRYGG | 1 cut(s) | 2259 |
| BstF5I | GGATG | 6 cut(s) | 370, 462, 843, 1210, 1771, 1956 |
| BstH2I | RGCGCY | 1 cut(s) | 415 |
| BstHHI | GCGC | 2 cut(s) | 414, 1473 |
| BstKTI | GATC | 9 cut(s) | 498, 703, 902, 913, 1097, 1324, 2011, 2414, 2851 |
| BstMAI | GTCTC | 3 cut(s) | 69, 1704, 1786 |
| BstMBI | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| BstMCI | CGRYCG | 1 cut(s) | 34 |
| BstNI | CCWGG | 4 cut(s) | 536, 897, 2013, 2771 |
| BstNSI | RCATGY | 5 cut(s) | 798, 935, 1346, 1811, 1974 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2433 |
| BstSCI | CCNGG | 6 cut(s) | 534, 895, 2011, 2539, 2769, 2828 |
| BstSFI | CTRYAG | 8 cut(s) | 471, 807, 1063, 1611, 1829, 1876, 1962, 2304 |
| BstSLI | GKGCMC | 2 cut(s) | 769, 2321 |
| BstV2I | GAAGAC | 5 cut(s) | 201, 497, 813, 2373, 2903 |
| BstX2I | RGATCY | 4 cut(s) | 899, 1094, 1321, 2008 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 733 |
| BstYI | RGATCY | 4 cut(s) | 899, 1094, 1321, 2008 |
| BsuRI | GGCC | 6 cut(s) | 203, 491, 747, 2628, 2769, 2833 |
| BtgI | CCRYGG | 1 cut(s) | 2259 |
| BtgZI | GCGATG | 1 cut(s) | 1663 |
| BtsCI | GGATG | 6 cut(s) | 370, 462, 843, 1210, 1771, 1956 |
| BtsI | GCAGTG | 3 cut(s) | 594, 611, 1971 |
| BtsIMutI | CAGTG | 7 cut(s) | 148, 224, 594, 611, 1971, 2445, 2938 |
| BveI | ACCTGC | 2 cut(s) | 2153, 2443 |
| Cac8I | GCNNGC | 9 cut(s) | 416, 668, 796, 2031, 2053, 2057, 2293, 2385, 2450 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1271 |
| CciI | TCATGA | 1 cut(s) | 181 |
| CfoI | GCGC | 2 cut(s) | 414, 1473 |
| Cfr10I | RCCGGY | 2 cut(s) | 414, 2843 |
| Cfr13I | GGNCC | 5 cut(s) | 841, 2435, 2567, 2627, 2767 |
| CseI | GACGC | 1 cut(s) | 491 |
| Csp6I | GTAC | 4 cut(s) | 28, 1154, 1580, 1653 |
| CviQI | GTAC | 4 cut(s) | 28, 1154, 1580, 1653 |
| DpnI | GATC | 9 cut(s) | 497, 702, 901, 912, 1096, 1323, 2010, 2413, 2850 |
| DpnII | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| DraI | TTTAAA | 2 cut(s) | 1278, 1548 |
| DraIII | CACNNNGTG | 1 cut(s) | 1898 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1110 |
| EaeI | YGGCCR | 2 cut(s) | 489, 2831 |
| Eam1104I | CTCTTC | 2 cut(s) | 512, 642 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1110 |
| EarI | CTCTTC | 2 cut(s) | 512, 642 |
| Ecl136II | GAGCTC | 1 cut(s) | 2826 |
| Eco130I | CCWWGG | 3 cut(s) | 1006, 1372, 2259 |
| Eco24I | GRGCYC | 1 cut(s) | 2828 |
| Eco47I | GGWCC | 3 cut(s) | 841, 2435, 2567 |
| Eco53kI | GAGCTC | 1 cut(s) | 2826 |
| Eco57I | CTGAAG | 1 cut(s) | 491 |
| Eco72I | CACGTG | 2 cut(s) | 434, 1787 |
| Eco88I | CYCGRG | 3 cut(s) | 443, 1480, 2181 |
| EcoICRI | GAGCTC | 1 cut(s) | 2826 |
| EcoRII | CCWGG | 4 cut(s) | 534, 895, 2011, 2769 |
| EcoT14I | CCWWGG | 3 cut(s) | 1006, 1372, 2259 |
| EcoT22I | ATGCAT | 2 cut(s) | 800, 1771 |
| EcoT38I | GRGCYC | 1 cut(s) | 2828 |
| ErhI | CCWWGG | 3 cut(s) | 1006, 1372, 2259 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1350, 1382, 2358, 2390 |
| FaqI | GGGAC | 2 cut(s) | 953, 2553 |
| FauI | CCCGC | 1 cut(s) | 411 |
| FauNDI | CATATG | 1 cut(s) | 1771 |
| FblI | GTMKAC | 2 cut(s) | 2427, 2957 |
| FokI | GGATG | 6 cut(s) | 377, 469, 850, 1197, 1778, 1963 |
| FriOI | GRGCYC | 1 cut(s) | 2828 |
| FspBI | CTAG | 3 cut(s) | 540, 1793, 2282 |
| GlaI | GCGC | 2 cut(s) | 413, 1472 |
| GsaI | CCCAGC | 1 cut(s) | 1911 |
| HaeII | RGCGCY | 1 cut(s) | 415 |
| HaeIII | GGCC | 6 cut(s) | 203, 491, 747, 2628, 2769, 2833 |
| HapII | CCGG | 5 cut(s) | 415, 2409, 2541, 2830, 2844 |
| HgaI | GACGC | 1 cut(s) | 491 |
| HhaI | GCGC | 2 cut(s) | 414, 1473 |
| Hin6I | GCGC | 2 cut(s) | 412, 1471 |
| HinP1I | GCGC | 2 cut(s) | 412, 1471 |
| HincII | GTYRAC | 3 cut(s) | 276, 1332, 2683 |
| HindII | GTYRAC | 3 cut(s) | 276, 1332, 2683 |
| HindIII | AAGCTT | 1 cut(s) | 1364 |
| HpaI | GTTAAC | 1 cut(s) | 276 |
| HpaII | CCGG | 5 cut(s) | 415, 2409, 2541, 2830, 2844 |
| HphI | GGTGA | 3 cut(s) | 710, 1151, 2858 |
| Hpy188I | TCNGA | 9 cut(s) | 220, 510, 631, 691, 783, 1207, 1338, 2002, 2274 |
| Hpy188III | TCNNGA | 6 cut(s) | 182, 445, 914, 1319, 2181, 2749 |
| Hpy99I | CGWCG | 1 cut(s) | 1227 |
| HpyAV | CCTTC | 7 cut(s) | 204, 302, 442, 1189, 1422, 1756, 2911 |
| HpyCH4III | ACNGT | 8 cut(s) | 32, 249, 1034, 1162, 1524, 2335, 2902, 2933 |
| HpyCH4IV | ACGT | 4 cut(s) | 433, 1630, 1786, 2203 |
| HpySE526I | ACGT | 4 cut(s) | 433, 1630, 1786, 2203 |
| HspAI | GCGC | 2 cut(s) | 412, 1471 |
| KroI | GCCGGC | 1 cut(s) | 414 |
| KroNI | GCCGGC | 1 cut(s) | 416 |
| KspAI | GTTAAC | 1 cut(s) | 276 |
| Kzo9I | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| LguI | GCTCTTC | 1 cut(s) | 642 |
| LmnI | GCTCC | 3 cut(s) | 1888, 2705, 2831 |
| MaeI | CTAG | 3 cut(s) | 540, 1793, 2282 |
| MaeII | ACGT | 4 cut(s) | 433, 1630, 1786, 2203 |
| MalI | GATC | 9 cut(s) | 497, 702, 901, 912, 1096, 1323, 2010, 2413, 2850 |
| MboI | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| MfeI | CAATTG | 2 cut(s) | 303, 641 |
| MflI | RGATCY | 4 cut(s) | 899, 1094, 1321, 2008 |
| MhlI | GDGCHC | 3 cut(s) | 769, 2321, 2828 |
| MlsI | TGGCCA | 1 cut(s) | 491 |
| MluNI | TGGCCA | 1 cut(s) | 491 |
| MlyI | GAGTC | 4 cut(s) | 646, 1067, 1417, 2899 |
| MmeI | TCCRAC | 3 cut(s) | 2128, 2200, 2326 |
| Mox20I | TGGCCA | 1 cut(s) | 491 |
| Mph1103I | ATGCAT | 2 cut(s) | 800, 1771 |
| MreI | CGCCGGCG | 1 cut(s) | 414 |
| MroNI | GCCGGC | 1 cut(s) | 414 |
| MroXI | GAANNNNTTC | 1 cut(s) | 924 |
| MscI | TGGCCA | 1 cut(s) | 491 |
| MslI | CAYNNNNRTG | 4 cut(s) | 731, 1182, 1812, 2891 |
| Msp20I | TGGCCA | 1 cut(s) | 491 |
| MspA1I | CMGCKG | 1 cut(s) | 476 |
| MspI | CCGG | 5 cut(s) | 415, 2409, 2541, 2830, 2844 |
| MspR9I | CCNGG | 6 cut(s) | 536, 897, 2013, 2541, 2771, 2830 |
| MunI | CAATTG | 2 cut(s) | 303, 641 |
| Mva1269I | GAATGC | 1 cut(s) | 2281 |
| MvaI | CCWGG | 4 cut(s) | 536, 897, 2013, 2771 |
| NaeI | GCCGGC | 1 cut(s) | 416 |
| NciI | CCSGG | 2 cut(s) | 2541, 2830 |
| NcoI | CCATGG | 1 cut(s) | 2259 |
| NdeI | CATATG | 1 cut(s) | 1771 |
| NdeII | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| NgoMIV | GCCGGC | 1 cut(s) | 414 |
| NlaIV | GGNNCC | 5 cut(s) | 1174, 1890, 2010, 2538, 2569 |
| NmeAIII | GCCGAG | 1 cut(s) | 1078 |
| NmuCI | GTSAC | 9 cut(s) | 122, 143, 914, 951, 1013, 1729, 1966, 2587, 2902 |
| NsiI | ATGCAT | 2 cut(s) | 800, 1771 |
| NspI | RCATGY | 5 cut(s) | 798, 935, 1346, 1811, 1974 |
| PaeI | GCATGC | 1 cut(s) | 798 |
| PaeR7I | CTCGAG | 1 cut(s) | 443 |
| PagI | TCATGA | 1 cut(s) | 181 |
| PaqCI | CACCTGC | 1 cut(s) | 2153 |
| PciI | ACATGT | 2 cut(s) | 931, 1970 |
| PciSI | GCTCTTC | 1 cut(s) | 642 |
| PctI | GAATGC | 1 cut(s) | 2281 |
| PdiI | GCCGGC | 1 cut(s) | 416 |
| PdmI | GAANNNNTTC | 1 cut(s) | 924 |
| PfeI | GAWTC | 8 cut(s) | 592, 626, 722, 1268, 1517, 1939, 2479, 2875 |
| PfoI | TCCNGGA | 1 cut(s) | 2539 |
| PleI | GAGTC | 4 cut(s) | 645, 1066, 1416, 2899 |
| PmaCI | CACGTG | 2 cut(s) | 434, 1787 |
| PmlI | CACGTG | 2 cut(s) | 434, 1787 |
| PpsI | GAGTC | 4 cut(s) | 645, 1066, 1416, 2899 |
| Ppu21I | YACGTR | 2 cut(s) | 434, 1787 |
| PscI | ACATGT | 2 cut(s) | 931, 1970 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2433 |
| PsiI | TTATAA | 3 cut(s) | 614, 1350, 2610 |
| Psp124BI | GAGCTC | 1 cut(s) | 2828 |
| Psp6I | CCWGG | 4 cut(s) | 534, 895, 2011, 2769 |
| PspCI | CACGTG | 2 cut(s) | 434, 1787 |
| PspFI | CCCAGC | 1 cut(s) | 1907 |
| PspGI | CCWGG | 4 cut(s) | 534, 895, 2011, 2769 |
| PspN4I | GGNNCC | 5 cut(s) | 1174, 1890, 2010, 2538, 2569 |
| PspPI | GGNCC | 5 cut(s) | 841, 2435, 2567, 2627, 2767 |
| PstI | CTGCAG | 6 cut(s) | 475, 811, 1615, 1833, 1966, 2308 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1271 |
| PsuI | RGATCY | 4 cut(s) | 899, 1094, 1321, 2008 |
| PvuII | CAGCTG | 1 cut(s) | 476 |
| RsaI | GTAC | 4 cut(s) | 29, 1155, 1581, 1654 |
| RsaNI | GTAC | 4 cut(s) | 28, 1154, 1580, 1653 |
| RseI | CAYNNNNRTG | 4 cut(s) | 731, 1182, 1812, 2891 |
| SacI | GAGCTC | 1 cut(s) | 2828 |
| SapI | GCTCTTC | 1 cut(s) | 642 |
| Sau3AI | GATC | 9 cut(s) | 495, 700, 899, 910, 1094, 1321, 2008, 2411, 2848 |
| Sau96I | GGNCC | 5 cut(s) | 841, 2435, 2567, 2627, 2767 |
| SchI | GAGTC | 4 cut(s) | 646, 1067, 1417, 2899 |
| ScrFI | CCNGG | 6 cut(s) | 536, 897, 2013, 2541, 2771, 2830 |
| SduI | GDGCHC | 3 cut(s) | 769, 2321, 2828 |
| SfcI | CTRYAG | 8 cut(s) | 471, 807, 1063, 1611, 1829, 1876, 1962, 2304 |
| Sfr274I | CTCGAG | 1 cut(s) | 443 |
| SgrAI | CRCCGGYG | 1 cut(s) | 414 |
| SinI | GGWCC | 3 cut(s) | 841, 2435, 2567 |
| SlaI | CTCGAG | 1 cut(s) | 443 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 731, 1182, 1812, 2891 |
| SmlI | CTYRAG | 4 cut(s) | 443, 845, 1575, 2396 |
| SmoI | CTYRAG | 4 cut(s) | 443, 845, 1575, 2396 |
| SphI | GCATGC | 1 cut(s) | 798 |
| SsiI | CCGC | 3 cut(s) | 44, 174, 418 |
| SspI | AATATT | 1 cut(s) | 2077 |
| SspMI | CTAG | 3 cut(s) | 540, 1793, 2282 |
| SstI | GAGCTC | 1 cut(s) | 2828 |
| StyD4I | CCNGG | 6 cut(s) | 534, 895, 2011, 2539, 2769, 2828 |
| StyI | CCWWGG | 3 cut(s) | 1006, 1372, 2259 |
| TaaI | ACNGT | 8 cut(s) | 32, 249, 1034, 1162, 1524, 2335, 2902, 2933 |
| TaiI | ACGT | 4 cut(s) | 436, 1633, 1789, 2206 |
| TaqI | TCGA | 6 cut(s) | 444, 720, 750, 1452, 1803, 2213 |
| TatI | WGTACW | 1 cut(s) | 1652 |
| TauI | GCSGC | 1 cut(s) | 46 |
| TfiI | GAWTC | 8 cut(s) | 592, 626, 722, 1268, 1517, 1939, 2479, 2875 |
| TscAI | CASTG | 7 cut(s) | 148, 231, 594, 611, 1971, 2445, 2938 |
| TseFI | GTSAC | 9 cut(s) | 122, 143, 914, 951, 1013, 1729, 1966, 2587, 2902 |
| Tsp45I | GTSAC | 9 cut(s) | 122, 143, 914, 951, 1013, 1729, 1966, 2587, 2902 |
| TspRI | CASTG | 7 cut(s) | 148, 231, 594, 611, 1971, 2445, 2938 |
| VneI | GTGCAC | 2 cut(s) | 765, 2317 |
| VpaK11BI | GGWCC | 3 cut(s) | 841, 2435, 2567 |
| XapI | RAATTY | 4 cut(s) | 113, 241, 1290, 2744 |
| XceI | RCATGY | 5 cut(s) | 798, 935, 1346, 1811, 1974 |
| XhoI | CTCGAG | 1 cut(s) | 443 |
| XmiI | GTMKAC | 2 cut(s) | 2427, 2957 |
| XmnI | GAANNNNTTC | 1 cut(s) | 924 |
| XspI | CTAG | 3 cut(s) | 540, 1793, 2282 |
| Zsp2I | ATGCAT | 2 cut(s) | 800, 1771 |