MD15G1309700.v1.1

Aldehyde dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000503
Physical Location & Seq
Reverse (-)
31090520 .. 31101562
11043 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1309700.v1.1.491

Sequence Viewer

Length: 3234 bp
ATGCTTCCTCCACAATCTGGAACATTTGAAGATCGTGAAGACCTCGTCAAATATGTTCGTGATTTTGGTGCCAGTCAAGGATATGTGGTGACCATCAAGAAATCGAGGAAAGACAGAAGAGTCATACTTGGTTGTGATAGAGGAGGTGTTTATCGCAATAGGCGTAAAATTGATGAAAGCAAGCGAAAAAGGAAAGCAAGTTCACGCCTTATAAATTGTCCTTTTGAAGCCATTGGGAAAAAGGAAGATGATTTATGGGTACTTACAGTAAAAAATGGGGAACACAACCATGAAGCATTAAAGGACATGTCAGAGCATCCTTATAGTCGTCGTTTTACTGAGGAAGAGGTTAGGCAAATCAAGCAAATGACTGAAGCTGGTGTAAAACCACGCCAAGTTCTTAAGGCCCTCAAGCAAATCAATCCTGAACTGCAGTCAACACCAAGGCATTTGTATAACCTCAAAGCCAAGATACGTCAGGGAAATCTATCAGAAAAAAGTTTCAAGTCATGGAGACCTGACCGATCTGCTCTGGTGAGTACAATTGCTACTGCCAGTGGGGAGTCGTTAAAGCAAAACAACCAACCGCTGAAGGTTCCTAATTTTATTGGAGGGAAATTCGTTGATTCACAAGGGTGTTCAGTCGTTGATGTTTTAAATCCAGCAACACAAGAGGTTGTTTCTCATGTTCCTTTAACTACCTATGAAGAGTTCAAAGCTGCAGTTAGTTCAGCCAAGCAAGCTTTTCCATCATGGAAAAACACTCCTATTACCACTCGTCAGCGTATCATGTTTAAGCTTCAGGAGCTCATCCGGAGAGATATCGACAAGCTTGCCATGAATATCACCATAGAACAGGGTAAGACATTGAAGGGAGCTGAGAGCGATGTTCTCCGTGGCTTAGAGGTCGTTGAACATGCTTGTGGAATGGCAACTCTACAAATTGGGGAGTTTGTTCCTAATGCGTCTAATGGCATTGACACATACTGCATTAGGGAACCACTAGGTGTTTGTGCCGGGATATGCCCCTTCAACTTTCCAGCAATGATTCCCTTGTGGATGTTCCCAATAGCAGTTACATGTGGCAATACATTTGTTCTTAAGCCATGTGAGAAAAATCCAGGGGCATCAATGATACTTGCAGCACTTGCAAAGGAGGCTGGTTTGCCTGATGGTGTCTTAAATATTGTTCATGGCACCCATGATACTATTAATTATATCTGTGATGATGATGACATAAAAGCTGTATCACTTGTTGGCTCAAATACAGCTGGGATGCACATACATGCAAGGGCAGTTGCTGGGGGAAAACTTGTTCAGTCCAGTATTGGGGGAAAAAATCATGCAATAATCATGCCTGATGCTAGCATGGATGCTACCTTGAATTCTCTGGTTACTGCTGGCTTTGGTGCTGCAGGACAAAGGTGTATGGCTCTCAACACAGCTGTTTTTATTGGAGGCTCCAGGCCATGGGAAGGCGAACTTGTGGATCGTGCCAAAGCCCTTAAAGTGAATGTTGGAACAGACCCTAGTGCAGACCTTGGTCCAGTTATTACGAAAGAGGTGAAGGATTCAATTTGCAGATTAGTTCAGACCAGTGTTGAAAGCGGCACTAGACTTATTCTTGATGGGAGAAATCTTACGGTTCCTGGATATGAGAATGGAAATTTTATTGGTCCTACGATCTTATGTGATGTTACAACCAACATGGATTGCTTTAAGGAAGAAATTTTTGGACCAGTTCTTCTTTGCATGCAGTCTTGTATTCGAGCTGCTAGCCTAGAAGAGGCCATATCCATCGTAAACAGAAACAGGTTTGGTAATGGAGCTTCCATATTCACAACATCTGGCATTGCTGCAAGGAAGTTCCAGAATGAAGTTGAGGCAGGGCTGGTTGGAATCAACGTCCCTGTTCCAATTCCTTTACCATTTTCCTCATCTAATGGATCAAAGGCATCTTTTGGTAGCAATCTCAATTTTTCTGGTAAGGCAGGTGTGCAGTTTTACACCCAGATCAAGACGGTTGCACAACAGTGGAAGGATTTACCTAGCCTAGAAGTGTCGCTGGGTCGGCCTCCATCATCTGAGACAGAGATGACAGGTAGAGGCGTGTCTTCGGGTTTGCCTTCAACATCTGAGAGAGATTCACCTAGCCAAAGAGTGTCAGCAGACATGCACTCAGAATCCGAGAGTGATTCACCAAGCCATGGAGCTCCACTGTCGATCACTCCCACGTCTGAGGCAGATCGACCTAACCCAGGAGTCTCATCAGTGTCAACAACCTATAGGAATTTGTCTAGTCAGGGAATTCCCCTCGTCATCCCAGCAACGTCTGAGAGAGATCTATCCAGTGCAGATATCTCTCTGGCTCTTCATCCAGAATCAGAAAGAGAAATACCAAGTCAAGTTGTGTCACTGAGACCTTCCCAGTCCTCAGAGAGGATATGTATGCCACAAATGTCTCATTGGACGGAAACCTCAACATCTCAAAGGACTGAAAATTTGCCTCAGACTTCTCATTGGATGAAAACTTCAAGATCAACGTCCTTAAGGACTGAAAATTTGCCTCCGACTTCTCAGTGGATGGAAACCTCAACACCAACATCTCAAAGGACTGAAAATATTCCTCCAAATTCTGAGAGAAATCATGTGCCTAAGTCTCAGAGGAATGGCAGCACGGCACTAACATCTCAAAGGATTGATACTACTATGGGATTGACTTCTGAGAGGGCATATGTGCCGACCTCTCATGACAACATGGTTCCAGTATCGCACAGAAATGACGGGATAAGCCTTACATCTCAAAGGATTGACGCAACCCTACATCCAACATCTGAGAGGGTATACATGCTGGCAGGATCTCATCTGAATGAGAACACCATTTTTTCAACATCCGAGAGGCTATACATGCCTGAAACATCTCATTGGCATGACCATATGGGTTCAACATCTCAGAGAACTGAAAGTACTCTACATCCAAATTCGGAGAGGATATACGTGTCAATAGCATCTCAAAGGAATGACGACTTGGCTGCAGCTTCTCACCGTGCTTCTGATGCTGTTCCATCCAGCTCGGAAAGATTATATACGTCTTCATTAGTTCAGAGAAATGCTGGTATACGACCAATTCCTGGTGCACCTCAGAGGATGTTTCCTCAAAACTCGATAGCTTCGATGGATGAATTTCCCAGCCAGGGAGCATCACTGACTTTACCACCGTCTCAGAGAATATAG

Protein Analysis

1078

Amino Acids

117.58

Weight (kDa)

8.19

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 207 - 675 2.5e-126 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14170
fragaria_vesca FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11410 FvH4_2g11410
malus_domestica MD09G1234200.v1.1 MD15G1309700.v1.1
prunus_persica Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1
pyrus_communis pycom09g15250 pycom15g27380
rosa_chinensis RchiOBHm_Chr2g0110691 RchiOBHm_Chr2g0137621 RchiOBHm_Chr6g0270041 RchiOBHm_Chr6g0270061 RchiOBHm_Chr6g0270141
rosa_laevigata RLG00000013815 RLG00000013819 RLG00000017831 RLG00000019609
rosa_multiflora Rmu_co8442727.1_g000001 Rmu_sc0000389.1_g000042 Rmu_sc0000918.1_g000033 Rmu_sc0003043.1_g000019 Rmu_sc0004376.1_g000013
rosa_roxburghii Rroxscaffold_2G00133030 Rroxscaffold_5G00350410 Rroxscaffold_5G00350420 Rroxscaffold_5G00350430 Rroxscaffold_6G00398360 Rroxscaffold_7G00198140 Rroxscaffold_7G00198180
rosa_rugosa Rorug02G0168700 Rorug02G0168800 Rorug02G0168800 Rorug02G0338300 Rorug06G0052100 Rorug06G0052200 Rorug06G0052800 Rorug06G0052900
rosa_samantha Rh2AG221000 Rh2AG387100 Rh2AG387200 Rh2AG547100 Rh2BG231100 Rh2BG504800 Rh2CG223400 Rh2CG374500 Rh2DG227200 Rh2DG410100 Rh2DG516200 Rh5CG251300 Rh6AG171200 Rh6AG171700 Rh6BG175700 Rh6BG176100 Rh6CG170500 Rh6CG171200 Rh6CG216400 Rh6DG162700 Rh6DG163000
rosa_wichuraiana Rw2G017050 Rw6G014700 Rw6G014740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 212
AarI CACCTGC 1 cut(s) 1982
AasI GACNNNNNNGTC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 1982
AccB1I GGYRCC 2 cut(s) 68, 1196
AccB7I CCANNNNNTGG 4 cut(s) 17, 1470, 2207, 3131
AccI GTMKAC 2 cut(s) 2844, 3118
AccIII TCCGGA 1 cut(s) 813
AciI CCGC 2 cut(s) 587, 1608
AclWI GGATC 3 cut(s) 1497, 1954, 2866
AcuI CTGAAG 3 cut(s) 393, 611, 785
AdeI CACNNNGTG 1 cut(s) 1007
AfaI GTAC 3 cut(s) 261, 541, 2968
AflII CTTAAG 3 cut(s) 401, 1100, 2548
AflIII ACRYGT 3 cut(s) 306, 1079, 2997
AjiI CACGTC 1 cut(s) 2235
AjnI CCWGG 6 cut(s) 1120, 1463, 1648, 2257, 3130, 3192
AjuI GAANNNNNNNTTGG 4 cut(s) 1716, 1748, 3011, 3043
AleI CACNNNNGTG 2 cut(s) 634, 2032
AloI GAACNNNNNNTCC 4 cut(s) 184, 216, 1299, 1331
Alw21I GWGCWC 3 cut(s) 810, 2215, 3139
Alw26I GTCTC 7 cut(s) 508, 2081, 2269, 2413, 2466, 2664, 3225
Alw44I GTGCAC 1 cut(s) 3135
AlwI GGATC 3 cut(s) 1497, 1954, 2866
AlwNI CAGNNNCTG 1 cut(s) 1301
Aor13HI TCCGGA 1 cut(s) 813
AoxI GGCC 4 cut(s) 405, 1466, 1788, 2072
ApaLI GTGCAC 1 cut(s) 3135
ApeKI GCWGC 8 cut(s) 719, 1142, 1412, 1772, 1856, 2673, 3032, 3035
ArsI GACNNNNNNTTYG 4 cut(s) 2485, 2517, 2545, 2577
AseI ATTAAT 1 cut(s) 1212
Asp700I GAANNNNTTC 1 cut(s) 2622
AspS9I GGNCC 4 cut(s) 406, 1544, 1676, 1736
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 7 cut(s) 100, 547, 838, 1576, 2139, 2190, 3035
AsuNHI GCTAGC 2 cut(s) 1364, 1775
AvaII GGWCC 3 cut(s) 1544, 1676, 1736
BaeGI GKGCMC 1 cut(s) 3139
BanI GGYRCC 2 cut(s) 68, 1196
BanII GRGCYC 2 cut(s) 810, 2215
BarI GAAGNNNNNNTAC 2 cut(s) 1813, 1845
BbsI GAAGAC 3 cut(s) 45, 2106, 3084
Bbv12I GWGCWC 3 cut(s) 810, 2215, 3139
BbvI GCAGC 8 cut(s) 706, 1154, 1399, 1759, 1843, 2685, 3019, 3047
BccI CCATC 9 cut(s) 101, 757, 1166, 1622, 1805, 2086, 2578, 3073, 3169
BceAI ACGGC 1 cut(s) 2694
BcgI CGANNNNNNTGC 4 cut(s) 815, 849, 3014, 3048
BciT130I CCWGG 6 cut(s) 1122, 1465, 1650, 2259, 3132, 3194
BcnI CCSGG 1 cut(s) 1018
BcoDI GTCTC 7 cut(s) 508, 2081, 2269, 2413, 2466, 2664, 3225
BfmI CTRYAG 5 cut(s) 431, 720, 1413, 2284, 3033
BfrI CTTAAG 3 cut(s) 401, 1100, 2548
BfuAI ACCTGC 1 cut(s) 1982
BglII AGATCT 1 cut(s) 2341
BisI GCNGC 9 cut(s) 720, 1143, 1413, 1609, 1773, 1857, 2674, 3033, 3036
BlsI GCNGC 9 cut(s) 721, 1144, 1414, 1610, 1774, 1858, 2675, 3034, 3037
BmcAI AGTACT 1 cut(s) 2968
Bme1390I CCNGG 7 cut(s) 1018, 1122, 1465, 1650, 2259, 3132, 3194
Bme18I GGWCC 3 cut(s) 1544, 1676, 1736
BmgBI CACGTC 1 cut(s) 2235
BmgT120I GGNCC 4 cut(s) 406, 1544, 1676, 1736
BmiI GGNNCC 7 cut(s) 70, 597, 999, 1198, 1462, 1647, 2763
BmrFI CCNGG 7 cut(s) 1018, 1122, 1465, 1650, 2259, 3132, 3194
BmrI ACTGGG 1 cut(s) 2422
BmsI GCATC 9 cut(s) 325, 1136, 1266, 1351, 1363, 1964, 3017, 3046, 3209
BmtI GCTAGC 2 cut(s) 1368, 1779
BmuI ACTGGG 1 cut(s) 2422
BoxI GACNNNNGTC 1 cut(s) 1542
BpiI GAAGAC 3 cut(s) 45, 2106, 3084
BpmI CTGGAG 1 cut(s) 1447
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 1018
BsaAI YACGTR 1 cut(s) 2998
BsaI GGTCTC 2 cut(s) 508, 2413
BsaJI CCNNGG 8 cut(s) 443, 895, 1121, 1469, 1540, 2206, 2257, 3193
BsaWI WCCGGW 1 cut(s) 813
Bse1I ACTGG 9 cut(s) 72, 555, 1323, 1547, 1596, 1739, 2349, 2428, 2765
Bse3DI GCAATG 2 cut(s) 1050, 1851
BseAI TCCGGA 1 cut(s) 813
BseBI CCWGG 6 cut(s) 1122, 1465, 1650, 2259, 3132, 3194
BseDI CCNNGG 8 cut(s) 443, 895, 1121, 1469, 1540, 2206, 2257, 3193
BseMI GCAATG 2 cut(s) 1050, 1851
BseNI ACTGG 9 cut(s) 72, 555, 1323, 1547, 1596, 1739, 2349, 2428, 2765
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 3139
BseXI GCAGC 8 cut(s) 706, 1154, 1399, 1759, 1843, 2685, 3019, 3047
BseYI CCCAGC 5 cut(s) 1271, 1301, 2065, 2323, 3188
BsgI GTGCAG 3 cut(s) 1554, 2018, 2373
BshFI GGCC 4 cut(s) 407, 1468, 1790, 2074
BshNI GGYRCC 2 cut(s) 68, 1196
BsiHKAI GWGCWC 3 cut(s) 810, 2215, 3139
BsiSI CCGG 2 cut(s) 814, 1017
BslFI GGGAC 1 cut(s) 1892
BsmAI GTCTC 7 cut(s) 508, 2081, 2269, 2413, 2466, 2664, 3225
BsmBI CGTCTC 1 cut(s) 3225
BsmFI GGGAC 1 cut(s) 1892
BsnI GGCC 4 cut(s) 407, 1468, 1790, 2074
Bso31I GGTCTC 2 cut(s) 508, 2413
Bsp1286I GDGCHC 3 cut(s) 810, 2215, 3139
Bsp13I TCCGGA 1 cut(s) 813
Bsp19I CCATGG 2 cut(s) 1469, 2206
BspACI CCGC 2 cut(s) 587, 1608
BspANI GGCC 4 cut(s) 407, 1468, 1790, 2074
BspEI TCCGGA 1 cut(s) 813
BspHI TCATGA 1 cut(s) 2749
BspLI GGNNCC 7 cut(s) 70, 597, 999, 1198, 1462, 1647, 2763
BspMAI CTGCAG 4 cut(s) 435, 724, 1417, 3037
BspMI ACCTGC 1 cut(s) 1982
BspOI GCTAGC 2 cut(s) 1368, 1779
BspPI GGATC 3 cut(s) 1497, 1954, 2866
BspQI GCTCTTC 1 cut(s) 2376
BspT107I GGYRCC 2 cut(s) 68, 1196
BspTI CTTAAG 3 cut(s) 401, 1100, 2548
BspTNI GGTCTC 2 cut(s) 508, 2413
BsrDI GCAATG 2 cut(s) 1050, 1851
BsrI ACTGG 9 cut(s) 72, 555, 1323, 1547, 1596, 1739, 2349, 2428, 2765
BssECI CCNNGG 8 cut(s) 443, 895, 1121, 1469, 1540, 2206, 2257, 3193
BssNAI GTATAC 2 cut(s) 2845, 3119
BssT1I CCWWGG 4 cut(s) 443, 1469, 1540, 2206
Bst1107I GTATAC 2 cut(s) 2845, 3119
Bst2UI CCWGG 6 cut(s) 1122, 1465, 1650, 2259, 3132, 3194
Bst4CI ACNGT 7 cut(s) 268, 1645, 2023, 2034, 2220, 3047, 3219
Bst6I CTCTTC 5 cut(s) 112, 339, 702, 1779, 2376
BstAFI CTTAAG 3 cut(s) 401, 1100, 2548
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstBAI YACGTR 1 cut(s) 2998
BstC8I GCNNGC 8 cut(s) 182, 741, 834, 1366, 1402, 1754, 1777, 2853
BstDSI CCRYGG 3 cut(s) 895, 1469, 2206
BstEII GGTNACC 1 cut(s) 88
BstENI CCTNNNNNAGG 3 cut(s) 1784, 2256, 2437
BstMAI GTCTC 7 cut(s) 508, 2081, 2269, 2413, 2466, 2664, 3225
BstMWI GCNNNNNNNGC 8 cut(s) 361, 740, 805, 1148, 1157, 2071, 2908, 3056
BstNI CCWGG 6 cut(s) 1122, 1465, 1650, 2259, 3132, 3194
BstNSI RCATGY 8 cut(s) 310, 920, 1083, 1289, 1756, 2176, 2851, 2911
BstPAI GACNNNNGTC 1 cut(s) 1542
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 7 cut(s) 1016, 1120, 1463, 1648, 2257, 3130, 3192
BstSFI CTRYAG 5 cut(s) 431, 720, 1413, 2284, 3033
BstSLI GKGCMC 1 cut(s) 3139
BstV1I GCAGC 8 cut(s) 706, 1154, 1399, 1759, 1843, 2685, 3019, 3047
BstV2I GAAGAC 3 cut(s) 45, 2106, 3084
BstX2I RGATCY 2 cut(s) 2341, 2858
BstYI RGATCY 2 cut(s) 2341, 2858
BstZ17I GTATAC 2 cut(s) 2845, 3119
BsuRI GGCC 4 cut(s) 407, 1468, 1790, 2074
BtgI CCRYGG 3 cut(s) 895, 1469, 2206
BtgZI GCGATG 1 cut(s) 900
BtrI CACGTC 1 cut(s) 2235
BtsIMutI CAGTG 9 cut(s) 562, 1603, 2039, 2216, 2277, 2356, 2414, 2585, 3203
BveI ACCTGC 1 cut(s) 1982
Cac8I GCNNGC 8 cut(s) 182, 741, 834, 1366, 1402, 1754, 1777, 2853
CaiI CAGNNNCTG 1 cut(s) 1301
CciI TCATGA 1 cut(s) 2749
Cfr13I GGNCC 4 cut(s) 406, 1544, 1676, 1736
CseI GACGC 2 cut(s) 954, 2822
Csp6I GTAC 3 cut(s) 260, 540, 2967
CviQI GTAC 3 cut(s) 260, 540, 2967
DraI TTTAAA 1 cut(s) 657
DraIII CACNNNGTG 1 cut(s) 1007
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 5 cut(s) 112, 339, 702, 1779, 2376
EarI CTCTTC 5 cut(s) 112, 339, 702, 1779, 2376
Ecl136II GAGCTC 2 cut(s) 808, 2213
Eco130I CCWWGG 4 cut(s) 443, 1469, 1540, 2206
Eco24I GRGCYC 2 cut(s) 810, 2215
Eco31I GGTCTC 2 cut(s) 508, 2413
Eco32I GATATC 2 cut(s) 823, 2359
Eco47I GGWCC 3 cut(s) 1544, 1676, 1736
Eco53kI GAGCTC 2 cut(s) 808, 2213
Eco57I CTGAAG 3 cut(s) 393, 611, 785
Eco91I GGTNACC 1 cut(s) 88
EcoICRI GAGCTC 2 cut(s) 808, 2213
EcoNI CCTNNNNNAGG 3 cut(s) 1784, 2256, 2437
EcoO109I RGGNCCY 1 cut(s) 406
EcoO65I GGTNACC 1 cut(s) 88
EcoRI GAATTC 2 cut(s) 1384, 2307
EcoRII CCWGG 6 cut(s) 1120, 1463, 1648, 2257, 3130, 3192
EcoRV GATATC 2 cut(s) 823, 2359
EcoT14I CCWWGG 4 cut(s) 443, 1469, 1540, 2206
EcoT38I GRGCYC 2 cut(s) 810, 2215
ErhI CCWWGG 4 cut(s) 443, 1469, 1540, 2206
Esp3I CGTCTC 1 cut(s) 3225
FalI AAGNNNNNCTT 2 cut(s) 1467, 1499
FaqI GGGAC 1 cut(s) 1892
FauNDI CATATG 2 cut(s) 2734, 2937
FblI GTMKAC 2 cut(s) 2844, 3118
Fnu4HI GCNGC 9 cut(s) 720, 1143, 1413, 1609, 1773, 1857, 2674, 3033, 3036
FriOI GRGCYC 2 cut(s) 810, 2215
Fsp4HI GCNGC 9 cut(s) 720, 1143, 1413, 1609, 1773, 1857, 2674, 3033, 3036
GluI GCNGC 9 cut(s) 720, 1143, 1413, 1609, 1773, 1857, 2674, 3033, 3036
GsaI CCCAGC 5 cut(s) 1275, 1305, 2069, 2327, 3192
GsuI CTGGAG 1 cut(s) 1447
HaeIII GGCC 4 cut(s) 407, 1468, 1790, 2074
HapII CCGG 2 cut(s) 814, 1017
HgaI GACGC 2 cut(s) 954, 2822
HincII GTYRAC 2 cut(s) 438, 2277
HindII GTYRAC 2 cut(s) 438, 2277
HindIII AAGCTT 3 cut(s) 741, 797, 830
HpaII CCGG 2 cut(s) 814, 1017
HphI GGTGA 7 cut(s) 100, 547, 838, 1576, 2139, 2190, 3035
Hpy166II GTNNAC 7 cut(s) 203, 438, 1804, 2277, 2845, 3119, 3137
Hpy8I GTNNAC 7 cut(s) 203, 438, 1804, 2277, 2845, 3119, 3137
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 8 cut(s) 586, 865, 1039, 1469, 1561, 2032, 2136, 2433
HpyCH4III ACNGT 7 cut(s) 268, 1645, 2023, 2034, 2220, 3047, 3219
HpyCH4IV ACGT 7 cut(s) 475, 1905, 2234, 2330, 2543, 2997, 3089
HpyF10VI GCNNNNNNNGC 8 cut(s) 361, 740, 805, 1148, 1157, 2071, 2908, 3056
HpySE526I ACGT 7 cut(s) 475, 1905, 2234, 2330, 2543, 2997, 3089
Kpn2I TCCGGA 1 cut(s) 813
LguI GCTCTTC 1 cut(s) 2376
LmnI GCTCC 7 cut(s) 805, 875, 1466, 1826, 2210, 2218, 3197
Lsp1109I GCAGC 8 cut(s) 706, 1154, 1399, 1759, 1843, 2685, 3019, 3047
LweI GCATC 9 cut(s) 325, 1136, 1266, 1351, 1363, 1964, 3017, 3046, 3209
MaeII ACGT 7 cut(s) 475, 1905, 2234, 2330, 2543, 2997, 3089
MaeIII GTNAC 5 cut(s) 88, 1075, 1393, 1696, 2412
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 2 cut(s) 2341, 2858
MhlI GDGCHC 3 cut(s) 810, 2215, 3139
MlyI GAGTC 3 cut(s) 129, 572, 2271
MmeI TCCRAC 4 cut(s) 1498, 1876, 2594, 2852
MroI TCCGGA 1 cut(s) 813
MroXI GAANNNNTTC 1 cut(s) 2622
MslI CAYNNNNRTG 8 cut(s) 288, 634, 921, 1284, 2032, 2778, 2868, 2928
MspA1I CMGCKG 3 cut(s) 589, 1271, 1445
MspCI CTTAAG 3 cut(s) 401, 1100, 2548
MspI CCGG 2 cut(s) 814, 1017
MspR9I CCNGG 7 cut(s) 1018, 1122, 1465, 1650, 2259, 3132, 3194
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 6 cut(s) 1122, 1465, 1650, 2259, 3132, 3194
MwoI GCNNNNNNNGC 8 cut(s) 361, 740, 805, 1148, 1157, 2071, 2908, 3056
NciI CCSGG 1 cut(s) 1018
NcoI CCATGG 2 cut(s) 1469, 2206
NdeI CATATG 2 cut(s) 2734, 2937
NheI GCTAGC 2 cut(s) 1364, 1775
NlaIV GGNNCC 7 cut(s) 70, 597, 999, 1198, 1462, 1647, 2763
NmuCI GTSAC 2 cut(s) 88, 2412
NspI RCATGY 8 cut(s) 310, 920, 1083, 1289, 1756, 2176, 2851, 2911
OliI CACNNNNGTG 2 cut(s) 634, 2032
PaeI GCATGC 1 cut(s) 1756
PagI TCATGA 1 cut(s) 2749
PaqCI CACCTGC 1 cut(s) 1982
PciI ACATGT 2 cut(s) 306, 1079
PciSI GCTCTTC 1 cut(s) 2376
PcsI WCGNNNNNNNCGW 2 cut(s) 160, 3170
PdmI GAANNNNTTC 1 cut(s) 2622
PfeI GAWTC 8 cut(s) 626, 1048, 1571, 1899, 2144, 2183, 2195, 2381
PflFI GACNNNGTC 1 cut(s) 44
PflMI CCANNNNNTGG 4 cut(s) 17, 1470, 2207, 3131
PfoI TCCNGGA 1 cut(s) 1648
PkrI GCNGC 9 cut(s) 721, 1144, 1414, 1610, 1774, 1858, 2675, 3034, 3037
PleI GAGTC 3 cut(s) 128, 571, 2270
PpsI GAGTC 3 cut(s) 128, 571, 2270
Ppu21I YACGTR 1 cut(s) 2998
PscI ACATGT 2 cut(s) 306, 1079
PshAI GACNNNNGTC 1 cut(s) 1542
PshBI ATTAAT 1 cut(s) 1212
PsiI TTATAA 1 cut(s) 212
Psp124BI GAGCTC 2 cut(s) 810, 2215
Psp6I CCWGG 6 cut(s) 1120, 1463, 1648, 2257, 3130, 3192
PspEI GGTNACC 1 cut(s) 88
PspFI CCCAGC 5 cut(s) 1271, 1301, 2065, 2323, 3188
PspGI CCWGG 6 cut(s) 1120, 1463, 1648, 2257, 3130, 3192
PspN4I GGNNCC 7 cut(s) 70, 597, 999, 1198, 1462, 1647, 2763
PspPI GGNCC 4 cut(s) 406, 1544, 1676, 1736
PsrI GAACNNNNNNTAC 2 cut(s) 2950, 2982
PstI CTGCAG 4 cut(s) 435, 724, 1417, 3037
PstNI CAGNNNCTG 1 cut(s) 1301
PsuI RGATCY 2 cut(s) 2341, 2858
PsyI GACNNNGTC 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 1271, 1445
RsaI GTAC 3 cut(s) 261, 541, 2968
RsaNI GTAC 3 cut(s) 260, 540, 2967
RseI CAYNNNNRTG 8 cut(s) 288, 634, 921, 1284, 2032, 2778, 2868, 2928
SacI GAGCTC 2 cut(s) 810, 2215
SapI GCTCTTC 1 cut(s) 2376
SatI GCNGC 9 cut(s) 720, 1143, 1413, 1609, 1773, 1857, 2674, 3033, 3036
Sau96I GGNCC 4 cut(s) 406, 1544, 1676, 1736
ScaI AGTACT 1 cut(s) 2968
SchI GAGTC 3 cut(s) 129, 572, 2271
ScrFI CCNGG 7 cut(s) 1018, 1122, 1465, 1650, 2259, 3132, 3194
SduI GDGCHC 3 cut(s) 810, 2215, 3139
SfaNI GCATC 9 cut(s) 325, 1136, 1266, 1351, 1363, 1964, 3017, 3046, 3209
SfcI CTRYAG 5 cut(s) 431, 720, 1413, 2284, 3033
SinI GGWCC 3 cut(s) 1544, 1676, 1736
SmiMI CAYNNNNRTG 8 cut(s) 288, 634, 921, 1284, 2032, 2778, 2868, 2928
SmlI CTYRAG 4 cut(s) 401, 410, 1100, 2548
SmoI CTYRAG 4 cut(s) 401, 410, 1100, 2548
SphI GCATGC 1 cut(s) 1756
SsiI CCGC 2 cut(s) 587, 1608
SspI AATATT 2 cut(s) 1186, 2623
SstI GAGCTC 2 cut(s) 810, 2215
StyD4I CCNGG 7 cut(s) 1016, 1120, 1463, 1648, 2257, 3130, 3192
StyI CCWWGG 4 cut(s) 443, 1469, 1540, 2206
TaaI ACNGT 7 cut(s) 268, 1645, 2023, 2034, 2220, 3047, 3219
TaiI ACGT 7 cut(s) 478, 1908, 2237, 2333, 2546, 3000, 3092
TaqI TCGA 7 cut(s) 104, 825, 1768, 2222, 2248, 3164, 3173
TaqII GACCGA 1 cut(s) 537
TatI WGTACW 2 cut(s) 539, 2966
TauI GCSGC 1 cut(s) 1611
TfiI GAWTC 8 cut(s) 626, 1048, 1571, 1899, 2144, 2183, 2195, 2381
TscAI CASTG 9 cut(s) 562, 1603, 2039, 2223, 2277, 2356, 2421, 2585, 3210
TseFI GTSAC 2 cut(s) 88, 2412
TseI GCWGC 8 cut(s) 719, 1142, 1412, 1772, 1856, 2673, 3032, 3035
Tsp45I GTSAC 2 cut(s) 88, 2412
TspGWI ACGGA 2 cut(s) 884, 2486
TspRI CASTG 9 cut(s) 562, 1603, 2039, 2223, 2277, 2356, 2421, 2585, 3210
Tth111I GACNNNGTC 1 cut(s) 44
Van91I CCANNNNNTGG 4 cut(s) 17, 1470, 2207, 3131
Vha464I CTTAAG 3 cut(s) 401, 1100, 2548
VneI GTGCAC 1 cut(s) 3135
VpaK11BI GGWCC 3 cut(s) 1544, 1676, 1736
VspI ATTAAT 1 cut(s) 1212
XagI CCTNNNNNAGG 3 cut(s) 1784, 2256, 2437
XceI RCATGY 8 cut(s) 310, 920, 1083, 1289, 1756, 2176, 2851, 2911
XmiI GTMKAC 2 cut(s) 2844, 3118
XmnI GAANNNNTTC 1 cut(s) 2622
ZrmI AGTACT 1 cut(s) 2968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.