RchiOBHm_Chr6g0270141

Methylmalonate-semialdehyde dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000503
Physical Location & Seq
Forward (+)
27924165 .. 27931408
7244 bp
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UTR
Exon/CDS
Intron
PRQ24234

Sequence Viewer

Length: 1614 bp
ATGTTGCAGCTTTCAATTCCAAGAGTGAGAAATCTCAAGGCTTTTAGAGCGACGATCTTTGCTGTCCGTAGCTCTCATTTCTCCACAGCGGCCGAGCCATCCTCGTCTTCTACTCCTCCGAGAGTTCCCAATCTAATTGGAGGTGCTTTTGTTAATTCACAATCCTCTGCGTCCATAGATGTCATAAATCCTGCCACGCAACAAGTTGTTTCGCAAGTTCCGTTGACCACAAATGAAGAGTTCAAAGCCGCAGTGTCTTCAGCAAAGAAGGCTTTTCAGTCGTGGCGTCACACACCGGTTACGACACGTCAGCGTGTCATGTTTAAGCTGCAAGAGCTTATCCGGAGAGATATTGACAAGTTAGCTTTGAATATTACTACAGAACAGGGGAAGACGTTAAAGGATGCGCATGGTGATGTGTTTCGTGGGCTAGAGGTGGTAGAACATGCATGTGGCACAGCGACTCTTCAAATGGGGGAATATGTTTCTAATGTATCAAATGGAATTGACACGTATAGCATTAGAGAGCCACTTGGTGTTTGTGCCGGGATTTGTCCGTTCAACTTTCCGGCAATGATTCCATTATGGATGTTCCCAATTGCAGTTGCCTGTGGGAATACCTTTATCCTAAAGCCATCAGAAAAAGATCCAGGTGCTTCTGTACTGCTAGCAGAGTTGGCAATGGAGGCTGGGTTGCCTGATGGTGTCTTAAATATTGTTCATGGAACCAATGATATTGTTAATGCAATTTGTGATGATGATGATATCAGAGCTGTATCATTTGTGGGTTCTAATACAGCTGGCATGCACATATATTCAAGAGCATCAGCTAAAGGGAAACGCGTTCAGTCCAATATGGGGGCAAAAAATCATGGGATTGTCATGCCTGATGCAAGTCCTGATGCTACTTTGAGTGCTCTAGTTGCCGCTGGTTTTGGTGCTGCAGGACAGAGGTGCATGGCACTCAGCACAGTTGTTTTCGTTGGAGGTTCAAAGTCATGGGAGAATAAACTTGTGGAGTACGGAAAATCACTGAAAGTAAGTTCAGGAACAGAACCTGATGCTGACCTTGGTCCTGTGATTAGCAAGCAAGCCAAGGAGCGCATATGCAATCTAATCCAATCTGGTGTTGAAAGTGGTGCCAAGCTACTGCTGGATGGGCGAGATATAGTGGTCCCAGGTTATGAGCATGGCAATTTTATTGGCCCTACTATCTTATCAAATGTTACAGCTGACATGGAGTGCTACAAGGAAGAAATTTTTGGTCCAGTTCTTCTTTGCTTGGAGGCTGACAGTCTAGAAGAGGCAATAAGCATTGTTAACAGAAACAGATATGGCAATGGCGCTTCTATATTTACTACATCTGGGGTAGCTGCTAGGAAATTTCAGACGGAGATTGAGGCTGGGCAGGTTGGTATCAACGTTCCTATTCCAGTTCCCTTGCCCTTTTTCTCATTTACTGGCAGCAAGGCATCTTTTGCAGGGGATTTGAACTTCTATGGCAAGGCAGGTGTTAACTTTTTCACCCAGATCAAAACAGTAACCCAGCAGTGGAAAAGTTTACCAGGCAGCAATGTAGTTAATCTTGCCATGCCAACTTCTCAAAAGGCATAG

Protein Analysis

537

Amino Acids

57.27

Weight (kDa)

7.07

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 50 - 514 9.5e-144 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14170
fragaria_vesca FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_1g19320 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11390 FvH4_2g11410 FvH4_2g11410
malus_domestica MD09G1234200.v1.1 MD15G1309700.v1.1
prunus_persica Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.6G205000_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073100_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1 Prupe.8G073300_v2.0.a1
pyrus_communis pycom09g15250 pycom15g27380
rosa_chinensis RchiOBHm_Chr2g0110691 RchiOBHm_Chr2g0137621 RchiOBHm_Chr6g0270041 RchiOBHm_Chr6g0270061 RchiOBHm_Chr6g0270141
rosa_laevigata RLG00000013815 RLG00000013819 RLG00000017831 RLG00000019609
rosa_multiflora Rmu_co8442727.1_g000001 Rmu_sc0000389.1_g000042 Rmu_sc0000918.1_g000033 Rmu_sc0003043.1_g000019 Rmu_sc0004376.1_g000013
rosa_roxburghii Rroxscaffold_2G00133030 Rroxscaffold_5G00350410 Rroxscaffold_5G00350420 Rroxscaffold_5G00350430 Rroxscaffold_6G00398360 Rroxscaffold_7G00198140 Rroxscaffold_7G00198180
rosa_rugosa Rorug02G0168700 Rorug02G0168800 Rorug02G0168800 Rorug02G0338300 Rorug06G0052100 Rorug06G0052200 Rorug06G0052800 Rorug06G0052900
rosa_samantha Rh2AG221000 Rh2AG387100 Rh2AG387200 Rh2AG547100 Rh2BG231100 Rh2BG504800 Rh2CG223400 Rh2CG374500 Rh2DG227200 Rh2DG410100 Rh2DG516200 Rh5CG251300 Rh6AG171200 Rh6AG171700 Rh6BG175700 Rh6BG176100 Rh6CG170500 Rh6CG171200 Rh6CG216400 Rh6DG162700 Rh6DG163000
rosa_wichuraiana Rw2G017050 Rw6G014700 Rw6G014740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1499
Acc16I TGCGCA 1 cut(s) 408
Acc36I ACCTGC 2 cut(s) 1399, 1499
AccB1I GGYRCC 1 cut(s) 1139
AccII CGCG 1 cut(s) 843
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 3 cut(s) 89, 249, 927
AclI AACGTT 1 cut(s) 1422
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 2 cut(s) 1257, 1382
AcuI CTGAAG 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 286
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 2 cut(s) 663, 1022
AfiI CCNNNNNNNGG 2 cut(s) 858, 1551
AflIII ACRYGT 3 cut(s) 305, 510, 841
AgeI ACCGGT 1 cut(s) 295
AgsI TTSAA 9 cut(s) 15, 244, 370, 470, 562, 819, 993, 1133, 1492
AjiI CACGTC 1 cut(s) 308
AjnI CCWGG 3 cut(s) 649, 1177, 1564
AjuI GAANNNNNNNTTGG 2 cut(s) 1245, 1277
Alw21I GWGCWC 1 cut(s) 919
AlwI GGATC 1 cut(s) 641
AlwNI CAGNNNCTG 1 cut(s) 1058
Aor13HI TCCGGA 1 cut(s) 342
AoxI GGCC 2 cut(s) 90, 1204
ApeKI GCWGC 6 cut(s) 7, 328, 941, 1373, 1464, 1569
ApoI RAATTY 2 cut(s) 1257, 1382
AsiGI ACCGGT 1 cut(s) 295
AspLEI GCGC 3 cut(s) 409, 1104, 1346
AspS9I GGNCC 4 cut(s) 1073, 1174, 1205, 1265
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 425, 1516
AsuNHI GCTAGC 1 cut(s) 667
AvaII GGWCC 3 cut(s) 1073, 1174, 1265
BanI GGYRCC 1 cut(s) 1139
BbsI GAAGAC 3 cut(s) 99, 249, 398
Bbv12I GWGCWC 1 cut(s) 919
BbvI GCAGC 6 cut(s) 19, 315, 928, 1360, 1476, 1581
BccI CCATC 4 cut(s) 106, 643, 695, 1151
BciT130I CCWGG 3 cut(s) 651, 1179, 1566
BcnI CCSGG 1 cut(s) 547
BfaI CTAG 5 cut(s) 431, 668, 920, 1298, 1377
BfmI CTRYAG 2 cut(s) 378, 942
BfoI RGCGCY 1 cut(s) 1347
BfuAI ACCTGC 2 cut(s) 1399, 1499
BisI GCNGC 9 cut(s) 8, 90, 249, 329, 927, 942, 1374, 1465, 1570
BlsI GCNGC 9 cut(s) 9, 91, 250, 330, 928, 943, 1375, 1466, 1571
Bme1390I CCNGG 4 cut(s) 547, 651, 1179, 1566
Bme18I GGWCC 3 cut(s) 1073, 1174, 1265
BmgBI CACGTC 1 cut(s) 308
BmgT120I GGNCC 4 cut(s) 1073, 1174, 1205, 1265
BmiI GGNNCC 3 cut(s) 727, 1141, 1176
BmrFI CCNGG 4 cut(s) 547, 651, 1179, 1566
BmsI GCATC 6 cut(s) 394, 833, 880, 892, 1051, 1481
BmtI GCTAGC 1 cut(s) 671
BoxI GACNNNNGTC 1 cut(s) 1071
BpiI GAAGAC 3 cut(s) 99, 249, 398
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 547
BsaAI YACGTR 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 1069, 1095, 1177
BsaWI WCCGGW 2 cut(s) 295, 342
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 2 cut(s) 858, 1551
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 3 cut(s) 1268, 1433, 1465
Bse3DI GCAATG 4 cut(s) 579, 687, 1345, 1579
BseAI TCCGGA 1 cut(s) 342
BseBI CCWGG 3 cut(s) 651, 1179, 1566
BseDI CCNNGG 3 cut(s) 1069, 1095, 1177
BseGI GGATG 4 cut(s) 98, 409, 594, 1162
BseLI CCNNNNNNNGG 2 cut(s) 858, 1551
BseMI GCAATG 4 cut(s) 579, 687, 1345, 1579
BseMII CTCAG 1 cut(s) 979
BseNI ACTGG 3 cut(s) 1268, 1433, 1465
BseRI GAGGAG 1 cut(s) 105
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 6 cut(s) 19, 315, 928, 1360, 1476, 1581
BseYI CCCAGC 3 cut(s) 689, 1403, 1545
Bsh1236I CGCG 1 cut(s) 843
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 92, 1206
BshNI GGYRCC 1 cut(s) 1139
BshTI ACCGGT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 919
BsiSI CCGG 4 cut(s) 296, 343, 546, 569
BslFI GGGAC 1 cut(s) 1160
BslI CCNNNNNNNGG 2 cut(s) 858, 1551
BsmFI GGGAC 1 cut(s) 1160
BsnI GGCC 2 cut(s) 92, 1206
Bsp1286I GDGCHC 1 cut(s) 919
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 3 cut(s) 54, 646, 1530
BspACI CCGC 3 cut(s) 89, 249, 927
BspANI GGCC 2 cut(s) 92, 1206
BspCNI CTCAG 1 cut(s) 978
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 843
BspLI GGNNCC 3 cut(s) 727, 1141, 1176
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 2 cut(s) 1399, 1499
BspOI GCTAGC 1 cut(s) 671
BspPI GGATC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 1139
BsrDI GCAATG 4 cut(s) 579, 687, 1345, 1579
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 3 cut(s) 1268, 1433, 1465
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 1069, 1095, 1177
BssMI GATC 3 cut(s) 54, 646, 1530
BssNI GRCGYC 1 cut(s) 286
BssT1I CCWWGG 2 cut(s) 1069, 1095
Bst2UI CCWGG 3 cut(s) 651, 1179, 1566
Bst4CI ACNGT 3 cut(s) 973, 1295, 1540
Bst6I CTCTTC 3 cut(s) 231, 471, 1296
BstACI GRCGYC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 1478
BstBAI YACGTR 1 cut(s) 513
BstC8I GCNNGC 5 cut(s) 669, 802, 806, 1088, 1092
BstDEI CTNAG 1 cut(s) 965
BstF5I GGATG 4 cut(s) 98, 409, 594, 1162
BstFNI CGCG 1 cut(s) 843
BstH2I RGCGCY 1 cut(s) 1347
BstHHI GCGC 3 cut(s) 409, 1104, 1346
BstKTI GATC 3 cut(s) 57, 649, 1533
BstMBI GATC 3 cut(s) 54, 646, 1530
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 8 cut(s) 47, 269, 334, 677, 686, 923, 1159, 1478
BstNI CCWGG 3 cut(s) 651, 1179, 1566
BstNSI RCATGY 3 cut(s) 449, 453, 808
BstPAI GACNNNNGTC 1 cut(s) 1071
BstSCI CCNGG 4 cut(s) 545, 649, 1177, 1564
BstSFI CTRYAG 2 cut(s) 378, 942
BstUI CGCG 1 cut(s) 843
BstV1I GCAGC 6 cut(s) 19, 315, 928, 1360, 1476, 1581
BstV2I GAAGAC 3 cut(s) 99, 249, 398
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
BstZI CGGCCG 1 cut(s) 90
BsuRI GGCC 2 cut(s) 92, 1206
BtrI CACGTC 1 cut(s) 308
BtsCI GGATG 4 cut(s) 98, 409, 594, 1162
BtsI GCAGTG 2 cut(s) 258, 1556
BtsIMutI CAGTG 3 cut(s) 258, 1031, 1556
BveI ACCTGC 2 cut(s) 1399, 1499
Cac8I GCNNGC 5 cut(s) 669, 802, 806, 1088, 1092
CaiI CAGNNNCTG 1 cut(s) 1058
CfoI GCGC 3 cut(s) 409, 1104, 1346
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 4 cut(s) 1073, 1174, 1205, 1265
CseI GACGC 2 cut(s) 159, 275
Csp6I GTAC 2 cut(s) 662, 1021
CspAI ACCGGT 1 cut(s) 295
CviQI GTAC 2 cut(s) 662, 1021
DdeI CTNAG 1 cut(s) 965
DpnI GATC 3 cut(s) 56, 648, 1532
DpnII GATC 3 cut(s) 54, 646, 1530
DraIII CACNNNGTG 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 90
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 3 cut(s) 231, 471, 1296
EarI CTCTTC 3 cut(s) 231, 471, 1296
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 1069, 1095
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 3 cut(s) 1073, 1174, 1265
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 3 cut(s) 649, 1177, 1564
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 2 cut(s) 1069, 1095
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 2 cut(s) 1069, 1095
FaqI GGGAC 1 cut(s) 1160
FauNDI CATATG 1 cut(s) 1106
Fnu4HI GCNGC 9 cut(s) 8, 90, 249, 329, 927, 942, 1374, 1465, 1570
FokI GGATG 4 cut(s) 85, 416, 601, 1169
Fsp4HI GCNGC 9 cut(s) 8, 90, 249, 329, 927, 942, 1374, 1465, 1570
FspAI RTGCGCAY 1 cut(s) 408
FspBI CTAG 5 cut(s) 431, 668, 920, 1298, 1377
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 3 cut(s) 408, 1103, 1345
GluI GCNGC 9 cut(s) 8, 90, 249, 329, 927, 942, 1374, 1465, 1570
GsaI CCCAGC 3 cut(s) 693, 1407, 1549
HaeII RGCGCY 1 cut(s) 1347
HaeIII GGCC 2 cut(s) 92, 1206
HapII CCGG 4 cut(s) 296, 343, 546, 569
HgaI GACGC 2 cut(s) 159, 275
HhaI GCGC 3 cut(s) 409, 1104, 1346
Hin1I GRCGYC 1 cut(s) 286
Hin6I GCGC 3 cut(s) 407, 1102, 1344
HinP1I GCGC 3 cut(s) 407, 1102, 1344
HincII GTYRAC 3 cut(s) 225, 1321, 1516
HindII GTYRAC 3 cut(s) 225, 1321, 1516
HinfI GANTC 2 cut(s) 463, 577
HpaI GTTAAC 2 cut(s) 1321, 1516
HpaII CCGG 4 cut(s) 296, 343, 546, 569
HphI GGTGA 2 cut(s) 425, 1516
Hpy166II GTNNAC 4 cut(s) 225, 1321, 1516, 1562
Hpy188I TCNGA 4 cut(s) 120, 640, 770, 1389
Hpy188III TCNNGA 5 cut(s) 343, 819, 899, 1047, 1298
Hpy8I GTNNAC 4 cut(s) 225, 1321, 1516, 1562
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 3 cut(s) 973, 1295, 1540
HpyCH4IV ACGT 4 cut(s) 307, 395, 512, 1422
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 269, 334, 677, 686, 923, 1159, 1478
HpyF3I CTNAG 1 cut(s) 965
HpySE526I ACGT 4 cut(s) 307, 395, 512, 1422
Hsp92I GRCGYC 1 cut(s) 286
HspAI GCGC 3 cut(s) 407, 1102, 1344
Kpn2I TCCGGA 1 cut(s) 342
KspAI GTTAAC 2 cut(s) 1321, 1516
Kzo9I GATC 3 cut(s) 54, 646, 1530
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 6 cut(s) 19, 315, 928, 1360, 1476, 1581
LweI GCATC 6 cut(s) 394, 833, 880, 892, 1051, 1481
MaeI CTAG 5 cut(s) 431, 668, 920, 1298, 1377
MaeII ACGT 4 cut(s) 307, 395, 512, 1422
MaeIII GTNAC 4 cut(s) 287, 298, 1225, 1540
MalI GATC 3 cut(s) 56, 648, 1532
MboI GATC 3 cut(s) 54, 646, 1530
MboII GAAGA 8 cut(s) 99, 248, 249, 403, 458, 1265, 1265, 1313
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 1 cut(s) 919
MluCI AATT 9 cut(s) 15, 135, 154, 504, 597, 747, 1195, 1257, 1382
MluI ACGCGT 1 cut(s) 841
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 1 cut(s) 964
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 342
MseI TTAA 8 cut(s) 153, 324, 398, 710, 741, 1320, 1515, 1581
MslI CAYNNNNRTG 2 cut(s) 414, 450
MspA1I CMGCKG 4 cut(s) 89, 800, 929, 1232
MspI CCGG 4 cut(s) 296, 343, 546, 569
MspR9I CCNGG 4 cut(s) 547, 651, 1179, 1566
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 3 cut(s) 651, 1179, 1566
MvnI CGCG 1 cut(s) 843
MwoI GCNNNNNNNGC 8 cut(s) 47, 269, 334, 677, 686, 923, 1159, 1478
NciI CCSGG 1 cut(s) 547
NdeI CATATG 1 cut(s) 1106
NdeII GATC 3 cut(s) 54, 646, 1530
NheI GCTAGC 1 cut(s) 667
NlaIV GGNNCC 3 cut(s) 727, 1141, 1176
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 287
NsbI TGCGCA 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 3 cut(s) 449, 453, 808
PaeI GCATGC 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 1499
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PfeI GAWTC 1 cut(s) 577
PinAI ACCGGT 1 cut(s) 295
PkrI GCNGC 9 cut(s) 9, 91, 250, 330, 928, 943, 1375, 1466, 1571
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 1071
Psp1406I AACGTT 1 cut(s) 1422
Psp6I CCWGG 3 cut(s) 649, 1177, 1564
PspFI CCCAGC 3 cut(s) 689, 1403, 1545
PspGI CCWGG 3 cut(s) 649, 1177, 1564
PspN4I GGNNCC 3 cut(s) 727, 1141, 1176
PspPI GGNCC 4 cut(s) 1073, 1174, 1205, 1265
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 2 cut(s) 800, 1232
RsaI GTAC 2 cut(s) 663, 1022
RsaNI GTAC 2 cut(s) 662, 1021
RseI CAYNNNNRTG 2 cut(s) 414, 450
SaqAI TTAA 8 cut(s) 153, 324, 398, 710, 741, 1320, 1515, 1581
SatI GCNGC 9 cut(s) 8, 90, 249, 329, 927, 942, 1374, 1465, 1570
Sau3AI GATC 3 cut(s) 54, 646, 1530
Sau96I GGNCC 4 cut(s) 1073, 1174, 1205, 1265
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 4 cut(s) 547, 651, 1179, 1566
SduI GDGCHC 1 cut(s) 919
SfaNI GCATC 6 cut(s) 394, 833, 880, 892, 1051, 1481
SfcI CTRYAG 2 cut(s) 378, 942
SinI GGWCC 3 cut(s) 1073, 1174, 1265
SmiMI CAYNNNNRTG 2 cut(s) 414, 450
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
SphI GCATGC 1 cut(s) 808
Sse9I AATT 9 cut(s) 15, 135, 154, 504, 597, 747, 1195, 1257, 1382
SsiI CCGC 3 cut(s) 89, 249, 927
SspI AATATT 2 cut(s) 373, 715
SspMI CTAG 5 cut(s) 431, 668, 920, 1298, 1377
StyD4I CCNGG 4 cut(s) 545, 649, 1177, 1564
StyI CCWWGG 2 cut(s) 1069, 1095
TaaI ACNGT 3 cut(s) 973, 1295, 1540
TaiI ACGT 4 cut(s) 310, 398, 515, 1425
TasI AATT 9 cut(s) 15, 135, 154, 504, 597, 747, 1195, 1257, 1382
TatI WGTACW 1 cut(s) 661
TauI GCSGC 3 cut(s) 92, 251, 929
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 8 cut(s) 153, 324, 398, 710, 741, 1320, 1515, 1581
Tru9I TTAA 8 cut(s) 153, 324, 398, 710, 741, 1320, 1515, 1581
TscAI CASTG 3 cut(s) 258, 1038, 1556
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 6 cut(s) 7, 328, 941, 1373, 1464, 1569
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 249, 710
TspGWI ACGGA 5 cut(s) 56, 210, 546, 1038, 1406
TspRI CASTG 3 cut(s) 258, 1038, 1556
VpaK11BI GGWCC 3 cut(s) 1073, 1174, 1265
XapI RAATTY 2 cut(s) 1257, 1382
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 3 cut(s) 449, 453, 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 1150
XspI CTAG 5 cut(s) 431, 668, 920, 1298, 1377
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.