Rw2G017060

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010487
Physical Location & Seq
Forward (+)
20797669 .. 20800293
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017060.1

Sequence Viewer

Length: 1020 bp
ATGCAATGCACGAGCATCATCTCAGTGCCCACAATCCACTCCAGAAATCTCAAACTTAGGAGGAACTCACACTCACACTCACAATTATCAGTTGCTCATCCTCAGAGCTACTTTGAAGACTATGATGATGTGAAGCTTCCTCTGCCTTCCCATGTCAAATTCATAACCAGCACTTCAAACCCATTCGTCAAACACTGCCACAAGCTTCGCCTGTCTTCTTCTTATCGCCACACTCATGGTTCAGTTCTCGTTGTGGGTGCTATACCCATCAGGGAAATATGCGAGTTTCAGAAGTCATTGCAAGAGAAAACTGTTATGATGGATTGTTTGCTTGTACCGGATAATTGTGAGGTTCCTGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTCCCGTGATGAAAAAGCTTTCGGGGATGCAATCAACTGAATCTATTGAAGCAATGGCTCTAATGAGAATACCTACGAGTTTTTCAGATATCAATGCTGATGAAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTCTGGTCCTTGATGGTGTTCAGGATCCTGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCCGGGTGTTGTGATCCATTCAATGAAAAAGCACTCCGAGCAAGCCGAGGAGCATCATTCCAGCTTCCTATTATTTCTGGAACTTGGAATCATCTTGAATCCCTCACAAGTGAATTCCAAATAAAGATGCTAGCTGGCCATCCAGAGACTGATAAAAAGTCGACGCCATTTCACCTTTCTCAAAACCTTGCAGATTCTCTTGCAACAGTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTCTGTCTGAGCAATCTCGACGGCAATGTGAGCTTGTAACCATTCCAATGGCAGGAGATTTTGAGTCGCTTAATGTTTCAGTTGCTGGTGGGATTTTCTTGTATATGTTGCAACCTAAAAACCATATATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

339

Amino Acids

37.47

Weight (kDa)

6.53

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 183 - 329 2.5e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 800
AclWI GGATC 3 cut(s) 566, 579, 647
AcoI YGGCCR 1 cut(s) 775
AcsI RAATTY 2 cut(s) 158, 752
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 2 cut(s) 336, 589
AfiI CCNNNNNNNGG 1 cut(s) 935
AgsI TTSAA 5 cut(s) 116, 177, 449, 661, 737
AjnI CCWGG 1 cut(s) 574
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 779
AlwI GGATC 3 cut(s) 566, 579, 647
AlwNI CAGNNNCTG 2 cut(s) 788, 968
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 1 cut(s) 775
ApoI RAATTY 2 cut(s) 158, 752
AspS9I GGNCC 1 cut(s) 553
AsuC2I CCSGG 2 cut(s) 642, 643
AsuHPI GGTGA 1 cut(s) 803
AsuNHI GCTAGC 1 cut(s) 769
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 30
BalI TGGCCA 1 cut(s) 777
BamHI GGATCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 401
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 2 cut(s) 123, 207
Bbv12I GWGCWC 1 cut(s) 401
BccI CCATC 6 cut(s) 275, 313, 541, 554, 612, 786
BceAI ACGGC 1 cut(s) 920
BciT130I CCWGG 1 cut(s) 576
BcnI CCSGG 2 cut(s) 642, 643
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 517, 606
Bme1390I CCNGG 3 cut(s) 576, 642, 643
Bme18I GGWCC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 3 cut(s) 354, 530, 573
BmrFI CCNGG 3 cut(s) 576, 642, 643
BmsI GCATC 4 cut(s) 24, 417, 701, 756
BmtI GCTAGC 1 cut(s) 773
BpiI GAAGAC 2 cut(s) 123, 207
BpmI CTGGAG 1 cut(s) 25
BpuMI CCSGG 2 cut(s) 642, 643
BsaHI GRCGYC 1 cut(s) 803
BsaJI CCNNGG 2 cut(s) 641, 685
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 1 cut(s) 935
Bse3DI GCAATG 4 cut(s) 11, 296, 459, 914
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 641, 685
BseGI GGATG 4 cut(s) 97, 432, 623, 778
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMI GCAATG 4 cut(s) 11, 296, 459, 914
BseMII CTCAG 5 cut(s) 36, 116, 610, 868, 882
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 1 cut(s) 30
BshFI GGCC 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 2 cut(s) 338, 642
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 1 cut(s) 779
BsnI GGCC 1 cut(s) 777
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 30, 401
Bsp143I GATC 3 cut(s) 571, 599, 652
BspANI GGCC 1 cut(s) 777
BspCNI CTCAG 5 cut(s) 35, 115, 609, 867, 883
BspLI GGNNCC 3 cut(s) 354, 530, 573
BspOI GCTAGC 1 cut(s) 773
BspPI GGATC 3 cut(s) 566, 579, 647
BsrDI GCAATG 4 cut(s) 11, 296, 459, 914
BssECI CCNNGG 2 cut(s) 641, 685
BssMI GATC 3 cut(s) 571, 599, 652
BssNI GRCGYC 1 cut(s) 803
BssSI CACGAG 1 cut(s) 10
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 313, 518, 847
BstACI GRCGYC 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 682, 771, 775
BstDEI CTNAG 6 cut(s) 22, 56, 102, 596, 854, 891
BstF5I GGATG 4 cut(s) 97, 432, 623, 778
BstKTI GATC 3 cut(s) 574, 602, 655
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 3 cut(s) 571, 599, 652
BstMWI GCNNNNNNNGC 3 cut(s) 142, 677, 913
BstNI CCWGG 1 cut(s) 576
BstSCI CCNGG 3 cut(s) 574, 640, 641
BstSFI CTRYAG 2 cut(s) 517, 606
BstSLI GKGCMC 1 cut(s) 30
BstV2I GAAGAC 2 cut(s) 123, 207
BstX2I RGATCY 1 cut(s) 571
BstXI CCANNNNNNTGG 2 cut(s) 236, 931
BstYI RGATCY 1 cut(s) 571
BsuRI GGCC 1 cut(s) 777
BtsCI GGATG 4 cut(s) 97, 432, 623, 778
BtsI GCAGTG 2 cut(s) 193, 590
BtsIMutI CAGTG 4 cut(s) 30, 193, 590, 852
Cac8I GCNNGC 3 cut(s) 682, 771, 775
CaiI CAGNNNCTG 2 cut(s) 788, 968
Cfr13I GGNCC 1 cut(s) 553
Cfr9I CCCGGG 1 cut(s) 641
CseI GACGC 1 cut(s) 811
Csp6I GTAC 2 cut(s) 335, 588
CspCI CAANNNNNGTGG 2 cut(s) 840, 875
CviAII CATG 2 cut(s) 152, 236
CviQI GTAC 2 cut(s) 335, 588
DdeI CTNAG 6 cut(s) 22, 56, 102, 596, 854, 891
DpnI GATC 3 cut(s) 573, 601, 654
DpnII GATC 3 cut(s) 571, 599, 652
EaeI YGGCCR 1 cut(s) 775
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 553
Eco53kI GAGCTC 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 641
EcoICRI GAGCTC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 401
FaeI CATG 2 cut(s) 155, 239
FatI CATG 2 cut(s) 151, 235
FblI GTMKAC 1 cut(s) 800
FokI GGATG 4 cut(s) 84, 439, 630, 765
FriOI GRGCYC 1 cut(s) 401
FspBI CTAG 1 cut(s) 770
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 1 cut(s) 777
HapII CCGG 2 cut(s) 338, 642
HgaI GACGC 1 cut(s) 811
Hin1I GRCGYC 1 cut(s) 803
Hin1II CATG 2 cut(s) 155, 239
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HindIII AAGCTT 3 cut(s) 134, 203, 416
HinfI GANTC 5 cut(s) 440, 727, 737, 833, 947
HpaII CCGG 2 cut(s) 338, 642
HphI GGTGA 1 cut(s) 803
Hpy166II GTNNAC 2 cut(s) 590, 801
Hpy188I TCNGA 6 cut(s) 105, 291, 487, 599, 677, 892
Hpy188III TCNNGA 7 cut(s) 42, 356, 569, 717, 734, 782, 900
Hpy8I GTNNAC 2 cut(s) 590, 801
Hpy99I CGWCG 2 cut(s) 805, 906
HpyAV CCTTC 2 cut(s) 156, 869
HpyCH4III ACNGT 3 cut(s) 313, 518, 847
HpyCH4V TGCA 8 cut(s) 4, 9, 301, 391, 430, 830, 842, 994
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 677, 913
HpyF3I CTNAG 6 cut(s) 22, 56, 102, 596, 854, 891
Hsp92I GRCGYC 1 cut(s) 803
Hsp92II CATG 2 cut(s) 155, 239
Kzo9I GATC 3 cut(s) 571, 599, 652
LmnI GCTCC 1 cut(s) 689
LweI GCATC 4 cut(s) 24, 417, 701, 756
MaeI CTAG 1 cut(s) 770
MaeIII GTNAC 1 cut(s) 919
MalI GATC 3 cut(s) 573, 601, 654
MboI GATC 3 cut(s) 571, 599, 652
MboII GAAGA 5 cut(s) 128, 207, 210, 626, 629
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 2 cut(s) 30, 401
MlsI TGGCCA 1 cut(s) 777
MluCI AATT 4 cut(s) 83, 158, 343, 752
MluNI TGGCCA 1 cut(s) 777
MlyI GAGTC 1 cut(s) 956
MnlI CCTC 7 cut(s) 54, 111, 150, 343, 502, 680, 752
Mox20I TGGCCA 1 cut(s) 777
MscI TGGCCA 1 cut(s) 777
MseI TTAA 1 cut(s) 954
MslI CAYNNNNRTG 3 cut(s) 23, 234, 929
Msp20I TGGCCA 1 cut(s) 777
MspI CCGG 2 cut(s) 338, 642
MspR9I CCNGG 3 cut(s) 576, 642, 643
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 142, 677, 913
NciI CCSGG 2 cut(s) 642, 643
NdeII GATC 3 cut(s) 571, 599, 652
NheI GCTAGC 1 cut(s) 769
NlaIII CATG 2 cut(s) 155, 239
NlaIV GGNNCC 3 cut(s) 354, 530, 573
NmeAIII GCCGAG 1 cut(s) 710
PfeI GAWTC 4 cut(s) 440, 727, 737, 833
PleI GAGTC 1 cut(s) 955
PpsI GAGTC 1 cut(s) 955
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 3 cut(s) 354, 530, 573
PspPI GGNCC 1 cut(s) 553
PstNI CAGNNNCTG 2 cut(s) 788, 968
PsuI RGATCY 1 cut(s) 571
RsaI GTAC 2 cut(s) 336, 589
RsaNI GTAC 2 cut(s) 335, 588
RseI CAYNNNNRTG 3 cut(s) 23, 234, 929
SacI GAGCTC 1 cut(s) 401
SalI GTCGAC 1 cut(s) 799
SaqAI TTAA 1 cut(s) 954
Sau3AI GATC 3 cut(s) 571, 599, 652
Sau96I GGNCC 1 cut(s) 553
SchI GAGTC 1 cut(s) 956
ScrFI CCNGG 3 cut(s) 576, 642, 643
SduI GDGCHC 2 cut(s) 30, 401
SfaNI GCATC 4 cut(s) 24, 417, 701, 756
SfcI CTRYAG 2 cut(s) 517, 606
SinI GGWCC 1 cut(s) 553
SmaI CCCGGG 1 cut(s) 643
SmiMI CAYNNNNRTG 3 cut(s) 23, 234, 929
Sse9I AATT 4 cut(s) 83, 158, 343, 752
SspMI CTAG 1 cut(s) 770
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 3 cut(s) 574, 640, 641
TaaI ACNGT 3 cut(s) 313, 518, 847
TaqI TCGA 3 cut(s) 544, 800, 901
TasI AATT 4 cut(s) 83, 158, 343, 752
TfiI GAWTC 4 cut(s) 440, 727, 737, 833
Tru1I TTAA 1 cut(s) 954
Tru9I TTAA 1 cut(s) 954
TscAI CASTG 4 cut(s) 30, 200, 597, 852
TspDTI ATGAA 4 cut(s) 151, 425, 516, 678
TspMI CCCGGG 1 cut(s) 641
TspRI CASTG 4 cut(s) 30, 200, 597, 852
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 2 cut(s) 158, 752
XmaI CCCGGG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 800
XspI CTAG 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.