Rroxscaffold_2G00133020

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010487
Physical Location & Seq
Reverse (-)
70103054 .. 70107167
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133020.1

Sequence Viewer

Length: 1212 bp
ATGGGATGCCGCCGTGCGAGCGGGAGGGGGACTAGGCGCGGGTGGAGGATAGATCAAAGGTTGGGAGGCTCCGGTGAGGAGGGCCGGATCGAAGGCCGAATGCGGGCGGTTGAGATCGGTGTTGCGAGTGGTCGGTGCCGCGATGACTCCGGTGAAGGGGAGGTGGGGGCAATAAATTCGCACCGGAATCCGGTCAACAGTCCGGTGACCGGTAGCCTGATTCAGGTCACCGGTCGCCCGATTCCCATCACCGGTCGCCGGAGTCCGAACCCGCCACCGGTCACCGAGCACAACAAGAGCAGCTTTGAAGACTATGATGATGTGAAGCTTCCTCTGCCTTCCCATGTCAAATTCATAACCAGCACTTCAAACCCATTCGTCAAACACTGCCATAAGCTTCGCCTGTCTTCTTCTTATCGCCACACTCATGGTTCAGTTCTCGTTGTGGGTGCTACACCTATCAGGGAAATATGCGAGTTTCAGAAGTCATTGCAAGAGAAAACTGTTATGATGGATTGTTTGCTTGTACCGGATAATTGTGAGGTTCCTGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTCCCGTGATGAAAAAGCTTTCGGGGATGCAATCAACTGAATCTATTGAAGCAATGGCTCTAATGAGAATACCTACGAGTTTTTCAGATATCAATGCTGATGAAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTCTGGTCCTTGATGGTGTTCAGGATCCGGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCCGGGTGTTGTGATCCATTCAATGAAAAAGCACTCCGAGCTAGCCGAGGAGCATCATTCCAGCTTCCTATTATTTCTGGAACTTGGAATCATCTTGAATCCCTCATAAATGAATTCCAAATAAAGATGCTAGCTGGCCATCCAGAGACTGATAAAAAGTCGACGCTATTTCATCTTTCTCAAAAACTTGCAGATTCTCTTGCAACAGTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTCTGTCTGAGCAATCTCGACGGCAATGTGAGCTTGTAACCATTCCAATGGCAGGAGATTTTGAGTCGCTTAATGTTTCAGTTGCTGGTGGGATTTTCTTGTATATGTTGCAACCTAAAAACCATATATCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

403

Amino Acids

44.11

Weight (kDa)

7.62

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 247 - 393 3.7e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 21
AccI GTMKAC 1 cut(s) 992
AccII CGCG 2 cut(s) 39, 141
AciI CCGC 7 cut(s) 10, 21, 39, 103, 107, 139, 272
AclWI GGATC 4 cut(s) 95, 758, 771, 839
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 175, 350, 944
AfaI GTAC 2 cut(s) 528, 781
AfiI CCNNNNNNNGG 9 cut(s) 103, 156, 190, 209, 223, 251, 258, 277, 1127
AgeI ACCGGT 4 cut(s) 209, 230, 251, 277
AgsI TTSAA 5 cut(s) 308, 369, 641, 853, 929
Alw21I GWGCWC 2 cut(s) 291, 593
Alw26I GTCTC 1 cut(s) 971
AlwI GGATC 4 cut(s) 95, 758, 771, 839
AlwNI CAGNNNCTG 2 cut(s) 980, 1160
Ama87I CYCGRG 1 cut(s) 833
AoxI GGCC 3 cut(s) 82, 94, 967
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 3 cut(s) 175, 350, 944
AsiGI ACCGGT 4 cut(s) 209, 230, 251, 277
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 82, 745
AsuC2I CCSGG 3 cut(s) 768, 834, 835
AsuHPI GGTGA 6 cut(s) 86, 164, 217, 220, 241, 274
AsuNHI GCTAGC 2 cut(s) 872, 961
AvaI CYCGRG 1 cut(s) 833
AvaII GGWCC 1 cut(s) 745
BalI TGGCCA 1 cut(s) 969
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 593
BbsI GAAGAC 2 cut(s) 315, 399
Bbv12I GWGCWC 2 cut(s) 291, 593
BbvI GCAGC 1 cut(s) 312
BccI CCATC 6 cut(s) 254, 505, 733, 746, 804, 978
BceAI ACGGC 1 cut(s) 1112
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BcnI CCSGG 3 cut(s) 768, 834, 835
BcoDI GTCTC 1 cut(s) 971
BfaI CTAG 3 cut(s) 33, 873, 962
BfmI CTRYAG 2 cut(s) 709, 798
BisI GCNGC 3 cut(s) 10, 139, 301
BlsI GCNGC 3 cut(s) 11, 140, 302
Bme1390I CCNGG 3 cut(s) 768, 834, 835
Bme18I GGWCC 1 cut(s) 745
BmeT110I CYCGRG 1 cut(s) 833
BmgT120I GGNCC 2 cut(s) 82, 745
BmiI GGNNCC 5 cut(s) 70, 137, 546, 722, 765
BmrFI CCNGG 3 cut(s) 768, 834, 835
BmsI GCATC 3 cut(s) 609, 893, 948
BmtI GCTAGC 2 cut(s) 876, 965
BpiI GAAGAC 2 cut(s) 315, 399
BpuMI CCSGG 3 cut(s) 768, 834, 835
BsaBI GATNNNNATC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 833, 877
Bsc4I CCNNNNNNNGG 9 cut(s) 103, 156, 190, 209, 223, 251, 258, 277, 1127
Bse118I RCCGGY 4 cut(s) 209, 230, 251, 277
Bse3DI GCAATG 3 cut(s) 488, 651, 1106
Bse8I GATNNNNATC 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 833, 877
BseGI GGATG 4 cut(s) 11, 624, 815, 970
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 9 cut(s) 103, 156, 190, 209, 223, 251, 258, 277, 1127
BseMI GCAATG 3 cut(s) 488, 651, 1106
BseMII CTCAG 3 cut(s) 802, 1060, 1074
BseRI GAGGAG 2 cut(s) 92, 894
BseXI GCAGC 1 cut(s) 312
Bsh1236I CGCG 2 cut(s) 39, 141
Bsh1285I CGRYCG 2 cut(s) 235, 256
BshFI GGCC 3 cut(s) 84, 96, 969
BshNI GGYRCC 1 cut(s) 135
BshTI ACCGGT 4 cut(s) 209, 230, 251, 277
BsiEI CGRYCG 2 cut(s) 235, 256
BsiHKAI GWGCWC 2 cut(s) 291, 593
BsiHKCI CYCGRG 1 cut(s) 833
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 9 cut(s) 103, 156, 190, 209, 223, 251, 258, 277, 1127
BsmAI GTCTC 1 cut(s) 971
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 3 cut(s) 84, 96, 969
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 2 cut(s) 291, 593
Bsp143I GATC 6 cut(s) 52, 87, 114, 763, 791, 844
BspACI CCGC 7 cut(s) 10, 21, 39, 103, 107, 139, 272
BspANI GGCC 3 cut(s) 84, 96, 969
BspCNI CTCAG 3 cut(s) 801, 1059, 1075
BspFNI CGCG 2 cut(s) 39, 141
BspLI GGNNCC 5 cut(s) 70, 137, 546, 722, 765
BspOI GCTAGC 2 cut(s) 876, 965
BspPI GGATC 4 cut(s) 95, 758, 771, 839
BspT107I GGYRCC 1 cut(s) 135
BsrBI CCGCTC 1 cut(s) 21
BsrDI GCAATG 3 cut(s) 488, 651, 1106
BsrFI RCCGGY 4 cut(s) 209, 230, 251, 277
BssAI RCCGGY 4 cut(s) 209, 230, 251, 277
BssECI CCNNGG 2 cut(s) 833, 877
BssMI GATC 6 cut(s) 52, 87, 114, 763, 791, 844
Bst4CI ACNGT 4 cut(s) 200, 505, 710, 1039
BstC8I GCNNGC 5 cut(s) 19, 105, 874, 963, 967
BstDEI CTNAG 3 cut(s) 788, 1046, 1083
BstEII GGTNACC 3 cut(s) 205, 226, 280
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 4 cut(s) 11, 624, 815, 970
BstFNI CGCG 2 cut(s) 39, 141
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 6 cut(s) 55, 90, 117, 766, 794, 847
BstMAI GTCTC 1 cut(s) 971
BstMBI GATC 6 cut(s) 52, 87, 114, 763, 791, 844
BstMCI CGRYCG 2 cut(s) 235, 256
BstMWI GCNNNNNNNGC 4 cut(s) 18, 334, 869, 1105
BstPI GGTNACC 3 cut(s) 205, 226, 280
BstSCI CCNGG 3 cut(s) 766, 832, 833
BstSFI CTRYAG 2 cut(s) 709, 798
BstUI CGCG 2 cut(s) 39, 141
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 2 cut(s) 315, 399
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 2 cut(s) 428, 1123
BstYI RGATCY 1 cut(s) 763
BsuRI GGCC 3 cut(s) 84, 96, 969
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 4 cut(s) 11, 624, 815, 970
BtsI GCAGTG 2 cut(s) 385, 782
BtsIMutI CAGTG 3 cut(s) 385, 782, 1044
Cac8I GCNNGC 5 cut(s) 19, 105, 874, 963, 967
CaiI CAGNNNCTG 2 cut(s) 980, 1160
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 4 cut(s) 209, 230, 251, 277
Cfr13I GGNCC 2 cut(s) 82, 745
Cfr9I CCCGGG 1 cut(s) 833
CseI GACGC 1 cut(s) 1003
Csp6I GTAC 2 cut(s) 527, 780
CspAI ACCGGT 4 cut(s) 209, 230, 251, 277
CspCI CAANNNNNGTGG 2 cut(s) 1032, 1067
CviAII CATG 2 cut(s) 344, 428
CviQI GTAC 2 cut(s) 527, 780
DdeI CTNAG 3 cut(s) 788, 1046, 1083
DpnI GATC 6 cut(s) 54, 89, 116, 765, 793, 846
DpnII GATC 6 cut(s) 52, 87, 114, 763, 791, 844
EaeI YGGCCR 1 cut(s) 967
Ecl136II GAGCTC 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 593
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 745
Eco53kI GAGCTC 1 cut(s) 591
Eco88I CYCGRG 1 cut(s) 833
Eco91I GGTNACC 3 cut(s) 205, 226, 280
EcoICRI GAGCTC 1 cut(s) 591
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoO65I GGTNACC 3 cut(s) 205, 226, 280
EcoRI GAATTC 1 cut(s) 944
EcoRV GATATC 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 593
FaeI CATG 2 cut(s) 347, 431
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 43
FatI CATG 2 cut(s) 343, 427
FauI CCCGC 4 cut(s) 14, 32, 96, 279
FblI GTMKAC 1 cut(s) 992
Fnu4HI GCNGC 3 cut(s) 10, 139, 301
FokI GGATG 4 cut(s) 18, 631, 822, 957
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 3 cut(s) 10, 139, 301
FspBI CTAG 3 cut(s) 33, 873, 962
GlaI GCGC 1 cut(s) 38
GluI GCNGC 3 cut(s) 10, 139, 301
HaeIII GGCC 3 cut(s) 84, 96, 969
HgaI GACGC 1 cut(s) 1003
HhaI GCGC 1 cut(s) 39
Hin1II CATG 2 cut(s) 347, 431
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 2 cut(s) 196, 993
HindII GTYRAC 2 cut(s) 196, 993
HindIII AAGCTT 3 cut(s) 326, 395, 608
HphI GGTGA 6 cut(s) 86, 164, 217, 220, 241, 274
Hpy166II GTNNAC 3 cut(s) 196, 782, 993
Hpy188I TCNGA 6 cut(s) 267, 483, 679, 791, 869, 1084
Hpy188III TCNNGA 6 cut(s) 548, 761, 909, 926, 974, 1092
Hpy8I GTNNAC 3 cut(s) 196, 782, 993
Hpy99I CGWCG 2 cut(s) 997, 1098
HpyAV CCTTC 4 cut(s) 86, 149, 348, 1061
HpyCH4III ACNGT 4 cut(s) 200, 505, 710, 1039
HpyCH4V TGCA 6 cut(s) 493, 583, 622, 1022, 1034, 1186
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 334, 869, 1105
HpyF3I CTNAG 3 cut(s) 788, 1046, 1083
Hsp92II CATG 2 cut(s) 347, 431
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 6 cut(s) 52, 87, 114, 763, 791, 844
LmnI GCTCC 2 cut(s) 74, 881
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 3 cut(s) 609, 893, 948
MaeI CTAG 3 cut(s) 33, 873, 962
MaeIII GTNAC 4 cut(s) 205, 226, 280, 1111
MalI GATC 6 cut(s) 54, 89, 116, 765, 793, 846
MbiI CCGCTC 1 cut(s) 21
MboI GATC 6 cut(s) 52, 87, 114, 763, 791, 844
MboII GAAGA 5 cut(s) 320, 399, 402, 818, 821
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 2 cut(s) 291, 593
MlsI TGGCCA 1 cut(s) 969
MluCI AATT 4 cut(s) 175, 350, 535, 944
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 3 cut(s) 140, 271, 1148
Mox20I TGGCCA 1 cut(s) 969
MscI TGGCCA 1 cut(s) 969
MseI TTAA 2 cut(s) 1146, 1210
MslI CAYNNNNRTG 2 cut(s) 426, 1121
Msp20I TGGCCA 1 cut(s) 969
MspR9I CCNGG 3 cut(s) 768, 834, 835
Mva1269I GAATGC 1 cut(s) 105
MvnI CGCG 2 cut(s) 39, 141
MwoI GCNNNNNNNGC 4 cut(s) 18, 334, 869, 1105
NciI CCSGG 3 cut(s) 768, 834, 835
NdeII GATC 6 cut(s) 52, 87, 114, 763, 791, 844
NheI GCTAGC 2 cut(s) 872, 961
NlaIII CATG 2 cut(s) 347, 431
NlaIV GGNNCC 5 cut(s) 70, 137, 546, 722, 765
NmeAIII GCCGAG 1 cut(s) 902
NmuCI GTSAC 3 cut(s) 205, 226, 280
PctI GAATGC 1 cut(s) 105
PfeI GAWTC 7 cut(s) 187, 220, 241, 632, 919, 929, 1025
PinAI ACCGGT 4 cut(s) 209, 230, 251, 277
PkrI GCNGC 3 cut(s) 11, 140, 302
PleI GAGTC 3 cut(s) 140, 270, 1147
PpsI GAGTC 3 cut(s) 140, 270, 1147
Psp124BI GAGCTC 1 cut(s) 593
PspEI GGTNACC 3 cut(s) 205, 226, 280
PspN4I GGNNCC 5 cut(s) 70, 137, 546, 722, 765
PspPI GGNCC 2 cut(s) 82, 745
PstNI CAGNNNCTG 2 cut(s) 980, 1160
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 2 cut(s) 528, 781
RsaNI GTAC 2 cut(s) 527, 780
RseI CAYNNNNRTG 2 cut(s) 426, 1121
SacI GAGCTC 1 cut(s) 593
SalI GTCGAC 1 cut(s) 991
SaqAI TTAA 2 cut(s) 1146, 1210
SatI GCNGC 3 cut(s) 10, 139, 301
Sau3AI GATC 6 cut(s) 52, 87, 114, 763, 791, 844
Sau96I GGNCC 2 cut(s) 82, 745
SchI GAGTC 3 cut(s) 140, 271, 1148
ScrFI CCNGG 3 cut(s) 768, 834, 835
SduI GDGCHC 2 cut(s) 291, 593
SfaNI GCATC 3 cut(s) 609, 893, 948
SfcI CTRYAG 2 cut(s) 709, 798
SinI GGWCC 1 cut(s) 745
SmaI CCCGGG 1 cut(s) 835
SmiMI CAYNNNNRTG 2 cut(s) 426, 1121
Sse9I AATT 4 cut(s) 175, 350, 535, 944
SsiI CCGC 7 cut(s) 10, 21, 39, 103, 107, 139, 272
SspMI CTAG 3 cut(s) 33, 873, 962
SstI GAGCTC 1 cut(s) 593
StyD4I CCNGG 3 cut(s) 766, 832, 833
TaaI ACNGT 4 cut(s) 200, 505, 710, 1039
TaqI TCGA 4 cut(s) 90, 736, 992, 1093
TasI AATT 4 cut(s) 175, 350, 535, 944
TauI GCSGC 2 cut(s) 12, 141
TfiI GAWTC 7 cut(s) 187, 220, 241, 632, 919, 929, 1025
Tru1I TTAA 2 cut(s) 1146, 1210
Tru9I TTAA 2 cut(s) 1146, 1210
TscAI CASTG 3 cut(s) 392, 789, 1044
TseFI GTSAC 3 cut(s) 205, 226, 280
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 3 cut(s) 205, 226, 280
TspDTI ATGAA 6 cut(s) 343, 617, 708, 870, 957, 992
TspMI CCCGGG 1 cut(s) 833
TspRI CASTG 3 cut(s) 392, 789, 1044
VpaK11BI GGWCC 1 cut(s) 745
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 3 cut(s) 175, 350, 944
XmaI CCCGGG 1 cut(s) 833
XmiI GTMKAC 1 cut(s) 992
XspI CTAG 3 cut(s) 33, 873, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.