Rorug02G0168900

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010487
Physical Location & Seq
Reverse (-)
15161559 .. 15165944
4386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0168900.1

Sequence Viewer

Length: 2784 bp
ATGTTTAGACCCCTCCTCAGACGTCTCAAATCCACCCTAGACTGCTTGAGGCCATTCATTGAAGAGATGGCAGAAGATAAGAAGCTATTGGATCGCCCAAAGGAGAAGCTAGAAAATCTTAGAATACAAATGGAGAATGGTGTAGAGCTCGTTCGCAAGTGCTCAAGTGTTCGTCGATGGACCAGCAACAAAAAGGACAAATACACCAACCAAGTATTTGAATTGGATGAACGTCTTCAAGGGCACTTAAGTATACTGATAATGGAGGTAGCAAGGGATGTGAGGGAGACCTCGGATTTGATAAGGAATATACAGAAGGTGATCAATAGAATCGAAGGGAGTGACTTTCAAGGTTCATGTGACATAACTGAACCTCCCATCCCTCCGGTTGAGTTAGAAGTATTGAACATGCAGAAAACAAGTAATGTGAAGGAGACACAGGTTTCAGAGGTCAATATAACTGAAGGGAGTGGTGTGGTACAAAATCGACTTGAATTTAAAGGTTCTTGTGATGTACTTGGACCTTCATCCCCTGCAGTTGGATTGGAAGAATCCAAGCATGGAAGAAAGGATTTGATCAAGGAGACATTGCAGAGAGAAAGTGTATTTCCCAAGAGACCGGGCCAGCCTGAGTGCCTGTTTTACATGAGGACAGGTGATTGTAAGTTCGGTGTAGATTGTAGATATCATCATCCAGACTGTGTTTTCAGTCCAACAGGCCTTCCTCTACAACCTGTTGTCTTCGTAATGAACCTGTACAATTTTCAACAGCAGGAAACAATGCCAAACTTTTCAGAACTGTCCAATATGAGTCCTCTTACAGTTGGATTGGACGTGCATTTGAAAGAATTGAAGAGAAAGCTTCTCAATCATGGGGAGTCATCAATTCTTGTTCTGACTGGCCCCGCTGGATGTGGAAAATCCACATTGGCAAAAGCTTTTTGTCAAGATGAAGAAGTTAAAGATAAATTCAAGGACAATATCTTCTTTGTTACGGTTTCGAAGAAGCTGAGCGTAGACCTCATTGCACTGCAAATACTATGTCAACTCAAGGATGAGGTACCTCAAGTTGAAAACCAAGTCACTGCAGTTGAGTGGCTGGGAAATTTTCTGAAAGAAGCAGGACAAAATCCTTTACTGTTGGTCCTGGATGATGTTTGGCATGTGTCAGAATTCCTTGATAACAACAATGAATTGAAAATAGAAAATCTCAAGATTTTGGTGACATCAAGATCTGAACTTCTAGGATTTGGTTCCCCATATTATATAAATGCATTGAGTGAAGAAGAGGCAATGACTCTAATTCGACGTTCAGCATTCCTGGAAGATAGGAGCTCTTATGTTCCAGAAGATATCCTGAGAAAGATAGTAAAGCACTGTAAGGGAAACGCACTTGCCCTTACAGTCATCGGAAGATCACTGTGCAGGATGCCAATAGATATCTGGCAAAAAAGAGTAGAGGAACTGTCGAAAGATTCTTCTATTTCTAACTGTGAGAGTGATCTGCTTTCTTGCCTCAAAATCAGTTATGATGCCTTGGCTGAAAATTCTGTTCTGAAGGAGTGTTTCTTGGACCTTTCTCTATTTCCTGAATACCAAAGAGTCCCTGCTGATGCCCTCACTGATATGTGGGCAGAGTTATATGGACTAGGTGAAAATATTCTACCCATTATGAACCTCCAAGAGCTCACTACCCGGGGTCTGGCTAAACTTGTAGGCACAAGGAAGGAGAAAGTGGAGGTGGATGGCTACTACAGTACACTCTGTTTTAACATGCACGATATTATAAGAGAGCTGGCTATCCATCTGACTAGTCAGAAACCGATAGACCAGAGAAACAGACTGCATATAGAAATTCTTGGAGGCAAGCTTCCTCACTGGATCAATCATCTTCTAGAACAGAAGTATCAGCCTATGAAGGCTCGCCTAGTATCGATCTCAACTGACGAAGTATTTTCACAAAAATGGCACAACATGCAACTACCAGAAGCAGAGGTTCTAGTTCTGAATTTTCAAACAGAGAATTATGCATTACCTGAATTTGTGCAAAACATGGGAAATTTGAAGGTTCTAATAGTCACAAGTTGTGGTACCTTACCTGCTTTCTGGCCTGTCGGTTTCTTACCTGCTCAATTGAGTAATTTTCAACTACTCAGTTCATTACCAAATCTGAGAACAATCAGACTAGAGGGCATTTCAATTTCTTCCATAAGCATGCACTCCATACAATTGAAGAGCCTGCAAAAGATATCTCTGTTCAACTGTCGCATTGGTCAAACTTTCAGCAACACTTCCATCCAACTTTCAGATGCATTTCCGAATCTGGTGGAAACAAATATTGACCACTGCAATGATTTGGTGACATTGCCTGGCATCTGTGATCTTGTTAATCTAAAGAAGCTAAGTCTCATCCGCTGTCATTATCTATCTGCTTTGCCTCCAAAGATCGGAAAGTTGTCCAAATTAGAAGTTTTAAGGCTGAAGTCATGCACAGTCTTAGAGATGTTGCCAGGTTCGATTGTGAAGCTCAATAAGTTAGTATTTCTTGACCTATCTTATTGCAAACGCCTTAAGGAGCTTCCTGATGTCATTGGACTGAGCAATTTGAAAACAATCGACATGAGACAGTGCTCAAGACTGCAAATGCTGCCACCCTCTGTTTTGTATCTTAAGCAGTTACAGGAAGTGATATGCGATGAAGAGACAGAGAAGTTATGGAAACCCTTGTTGCCCCGCCTCAAAAACCTGCGCATTAAGTTTAGGTTTCCCGAGATTCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

927

Amino Acids

105.66

Weight (kDa)

6.68

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 1 - 96 7.7e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
zf-CCCH PF00642 207 - 232 1.2e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
NB-ARC PF00931 280 - 440 1.9e-19 NB-ARC domain
ATPase_2 PF01637 281 - 453 1.5e-07 ATPase domain predominantly from Archaea
NPHP3_N PF24883 286 - 362 9.6e-06 Nephrocystin 3, N-terminal
AAA_22 PF13401 294 - 399 5.8e-08 AAA domain
LRR_14 PF23598 779 - 865 8.9e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1787
AatII GACGTC 1 cut(s) 25
Acc16I TGCGCA 1 cut(s) 2750
Acc36I ACCTGC 3 cut(s) 2107, 2134, 2754
Acc65I GGTACC 2 cut(s) 1060, 2090
AccB1I GGYRCC 2 cut(s) 1060, 2090
AccI GTMKAC 2 cut(s) 253, 1017
AciI CCGC 3 cut(s) 906, 2413, 2734
AclWI GGATC 2 cut(s) 99, 1889
AcsI RAATTY 9 cut(s) 494, 968, 1105, 1172, 1546, 1854, 2008, 2039, 2059
AcuI CTGAAG 3 cut(s) 483, 1577, 2501
AcyI GRCGYC 1 cut(s) 22
AfaI GTAC 6 cut(s) 480, 516, 758, 1062, 1759, 2092
AfiI CCNNNNNNNGG 3 cut(s) 539, 1702, 2447
AflII CTTAAG 3 cut(s) 247, 2570, 2669
AhlI ACTAGT 1 cut(s) 1811
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 4 cut(s) 1146, 1320, 2368, 2509
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
Alw21I GWGCWC 5 cut(s) 150, 164, 1337, 1689, 2633
Alw26I GTCTC 8 cut(s) 29, 281, 428, 578, 610, 2411, 2617, 2696
AlwI GGATC 2 cut(s) 99, 1889
Ama87I CYCGRG 2 cut(s) 1695, 2768
AoxI GGCC 5 cut(s) 50, 622, 718, 901, 2108
ApeKI GCWGC 1 cut(s) 2647
ApoI RAATTY 9 cut(s) 494, 968, 1105, 1172, 1546, 1854, 2008, 2039, 2059
ArsI GACNNNNNNTTYG 4 cut(s) 280, 312, 1027, 1059
Asp700I GAANNNNTTC 2 cut(s) 234, 1659
Asp718I GGTACC 2 cut(s) 1060, 2090
AspLEI GCGC 1 cut(s) 2751
AspS9I GGNCC 6 cut(s) 180, 521, 622, 902, 1144, 1573
AsuC2I CCSGG 3 cut(s) 621, 1696, 1697
AsuHPI GGTGA 5 cut(s) 331, 668, 1234, 1664, 2371
AsuII TTCGAA 1 cut(s) 1001
AvaI CYCGRG 2 cut(s) 1695, 2768
AvaII GGWCC 4 cut(s) 180, 521, 1144, 1573
BaeGI GKGCMC 1 cut(s) 246
BaeI ACNNNNGTAYC 2 cut(s) 1889, 1922
BanI GGYRCC 2 cut(s) 1060, 2090
BanII GRGCYC 3 cut(s) 150, 1337, 1689
BbsI GAAGAC 2 cut(s) 227, 733
Bbv12I GWGCWC 5 cut(s) 150, 164, 1337, 1689, 2633
BbvI GCAGC 1 cut(s) 2634
BccI CCATC 6 cut(s) 61, 171, 386, 1739, 1812, 2303
BciT130I CCWGG 4 cut(s) 1148, 1322, 2370, 2511
BclI TGATCA 2 cut(s) 321, 576
BcnI CCSGG 3 cut(s) 621, 1696, 1697
BcoDI GTCTC 8 cut(s) 29, 281, 428, 578, 610, 2411, 2617, 2696
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 9 cut(s) 38, 110, 1244, 1649, 1812, 1895, 1928, 2000, 2186
BfmI CTRYAG 3 cut(s) 534, 1086, 1753
BfrI CTTAAG 3 cut(s) 247, 2570, 2669
BfuAI ACCTGC 3 cut(s) 2107, 2134, 2754
BglII AGATCT 1 cut(s) 1232
BisI GCNGC 1 cut(s) 2648
BlpI GCTNAGC 1 cut(s) 1010
BlsI GCNGC 1 cut(s) 2649
Bme1390I CCNGG 7 cut(s) 621, 1148, 1322, 1696, 1697, 2370, 2511
Bme18I GGWCC 4 cut(s) 180, 521, 1144, 1573
BmeT110I CYCGRG 2 cut(s) 1695, 2768
BmgBI CACGTC 1 cut(s) 835
BmgT120I GGNCC 6 cut(s) 180, 521, 622, 902, 1144, 1573
BmiI GGNNCC 4 cut(s) 904, 1062, 1255, 2092
BmrFI CCNGG 7 cut(s) 621, 1148, 1322, 1696, 1697, 2370, 2511
BmsI GCATC 5 cut(s) 1419, 1522, 1603, 2299, 2382
BpiI GAAGAC 2 cut(s) 227, 733
Bpu1102I GCTNAGC 1 cut(s) 1010
Bpu14I TTCGAA 1 cut(s) 1001
BpuEI CTTGAG 6 cut(s) 67, 148, 1034, 1050, 1196, 2617
BpuMI CCSGG 3 cut(s) 621, 1696, 1697
Bsa29I ATCGAT 1 cut(s) 1934
BsaHI GRCGYC 1 cut(s) 22
BsaI GGTCTC 2 cut(s) 281, 610
BsaJI CCNNGG 4 cut(s) 291, 1536, 1695, 1696
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 1702, 2447
Bse1I ACTGG 2 cut(s) 904, 1883
Bse3DI GCAATG 5 cut(s) 587, 1023, 1299, 2356, 2363
BseBI CCWGG 4 cut(s) 1148, 1322, 2370, 2511
BseCI ATCGAT 1 cut(s) 1934
BseDI CCNNGG 4 cut(s) 291, 1536, 1695, 1696
BseLI CCNNNNNNNGG 3 cut(s) 539, 1702, 2447
BseMI GCAATG 5 cut(s) 587, 1023, 1299, 2356, 2363
BseMII CTCAG 7 cut(s) 31, 621, 1001, 1349, 2162, 2167, 2588
BseNI ACTGG 2 cut(s) 904, 1883
BseRI GAGGAG 1 cut(s) 5
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 1 cut(s) 2634
BseYI CCCAGC 1 cut(s) 1099
BsgI GTGCAG 1 cut(s) 1444
BshFI GGCC 5 cut(s) 52, 624, 720, 903, 2110
BshNI GGYRCC 2 cut(s) 1060, 2090
BshVI ATCGAT 1 cut(s) 1934
BsiHKAI GWGCWC 5 cut(s) 150, 164, 1337, 1689, 2633
BsiHKCI CYCGRG 2 cut(s) 1695, 2768
BsiSI CCGG 3 cut(s) 386, 620, 1696
BslFI GGGAC 1 cut(s) 1589
BslI CCNNNNNNNGG 3 cut(s) 539, 1702, 2447
BsmAI GTCTC 8 cut(s) 29, 281, 428, 578, 610, 2411, 2617, 2696
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 1589
BsmI GAATGC 1 cut(s) 1316
BsnI GGCC 5 cut(s) 52, 624, 720, 903, 2110
Bso31I GGTCTC 2 cut(s) 281, 610
BsoBI CYCGRG 2 cut(s) 1695, 2768
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 6 cut(s) 150, 164, 246, 1337, 1689, 2633
Bsp1407I TGTACA 1 cut(s) 756
Bsp1720I GCTNAGC 1 cut(s) 1010
BspACI CCGC 3 cut(s) 906, 2413, 2734
BspANI GGCC 5 cut(s) 52, 624, 720, 903, 2110
BspCNI CTCAG 7 cut(s) 30, 622, 1002, 1350, 2163, 2166, 2589
BspDI ATCGAT 1 cut(s) 1934
BspLI GGNNCC 4 cut(s) 904, 1062, 1255, 2092
BspMAI CTGCAG 2 cut(s) 538, 1090
BspMI ACCTGC 3 cut(s) 2107, 2134, 2754
BspPI GGATC 2 cut(s) 99, 1889
BspQI GCTCTTC 1 cut(s) 2228
BspT104I TTCGAA 1 cut(s) 1001
BspT107I GGYRCC 2 cut(s) 1060, 2090
BspTI CTTAAG 3 cut(s) 247, 2570, 2669
BspTNI GGTCTC 2 cut(s) 281, 610
BsrDI GCAATG 5 cut(s) 587, 1023, 1299, 2356, 2363
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 2 cut(s) 904, 1883
BssECI CCNNGG 4 cut(s) 291, 1536, 1695, 1696
BssNAI GTATAC 1 cut(s) 254
BssNI GRCGYC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 1536
Bst1107I GTATAC 1 cut(s) 254
Bst2UI CCWGG 4 cut(s) 1148, 1322, 2370, 2511
Bst6I CTCTTC 5 cut(s) 57, 848, 1281, 2228, 2694
BstACI GRCGYC 1 cut(s) 22
BstAFI CTTAAG 3 cut(s) 247, 2570, 2669
BstAPI GCANNNNNTGC 2 cut(s) 1975, 2647
BstAUI TGTACA 1 cut(s) 756
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 6 cut(s) 626, 1797, 1868, 1924, 2216, 2240
BstHHI GCGC 1 cut(s) 2751
BstMAI GTCTC 8 cut(s) 29, 281, 428, 578, 610, 2411, 2617, 2696
BstMWI GCNNNNNNNGC 2 cut(s) 1975, 2647
BstNI CCWGG 4 cut(s) 1148, 1322, 2370, 2511
BstNSI RCATGY 5 cut(s) 412, 1166, 1777, 1978, 2218
BstSCI CCNGG 7 cut(s) 619, 1146, 1320, 1694, 1695, 2368, 2509
BstSFI CTRYAG 3 cut(s) 534, 1086, 1753
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 1 cut(s) 2634
BstV2I GAAGAC 2 cut(s) 227, 733
BstX2I RGATCY 1 cut(s) 1232
BstYI RGATCY 1 cut(s) 1232
BstZ17I GTATAC 1 cut(s) 254
Bsu15I ATCGAT 1 cut(s) 1934
BsuRI GGCC 5 cut(s) 52, 624, 720, 903, 2110
BsuTUI ATCGAT 1 cut(s) 1934
BtgZI GCGATG 1 cut(s) 2709
BtrI CACGTC 1 cut(s) 835
BtsI GCAGTG 3 cut(s) 1028, 1083, 2344
BtsIMutI CAGTG 8 cut(s) 1028, 1083, 1375, 1418, 1620, 1876, 2344, 2633
BveI ACCTGC 3 cut(s) 2107, 2134, 2754
Cac8I GCNNGC 6 cut(s) 626, 1797, 1868, 1924, 2216, 2240
CfoI GCGC 1 cut(s) 2751
Cfr13I GGNCC 6 cut(s) 180, 521, 622, 902, 1144, 1573
Cfr9I CCCGGG 1 cut(s) 1695
ClaI ATCGAT 1 cut(s) 1934
Csp6I GTAC 6 cut(s) 479, 515, 757, 1061, 1758, 2091
CviQI GTAC 6 cut(s) 479, 515, 757, 1061, 1758, 2091
DraI TTTAAA 1 cut(s) 499
Eam1104I CTCTTC 5 cut(s) 57, 848, 1281, 2228, 2694
EarI CTCTTC 5 cut(s) 57, 848, 1281, 2228, 2694
Ecl136II GAGCTC 3 cut(s) 148, 1335, 1687
Eco130I CCWWGG 1 cut(s) 1536
Eco147I AGGCCT 1 cut(s) 720
Eco24I GRGCYC 3 cut(s) 150, 1337, 1689
Eco31I GGTCTC 2 cut(s) 281, 610
Eco32I GATATC 4 cut(s) 686, 1354, 1441, 2250
Eco47I GGWCC 4 cut(s) 180, 521, 1144, 1573
Eco53kI GAGCTC 3 cut(s) 148, 1335, 1687
Eco57I CTGAAG 3 cut(s) 483, 1577, 2501
Eco88I CYCGRG 2 cut(s) 1695, 2768
EcoICRI GAGCTC 3 cut(s) 148, 1335, 1687
EcoRI GAATTC 1 cut(s) 1172
EcoRII CCWGG 4 cut(s) 1146, 1320, 2368, 2509
EcoRV GATATC 4 cut(s) 686, 1354, 1441, 2250
EcoT14I CCWWGG 1 cut(s) 1536
EcoT22I ATGCAT 3 cut(s) 1276, 2032, 2314
EcoT38I GRGCYC 3 cut(s) 150, 1337, 1689
ErhI CCWWGG 1 cut(s) 1536
Esp3I CGTCTC 1 cut(s) 29
FaqI GGGAC 1 cut(s) 1589
FauI CCCGC 2 cut(s) 913, 2741
FbaI TGATCA 2 cut(s) 321, 576
FblI GTMKAC 2 cut(s) 253, 1017
Fnu4HI GCNGC 1 cut(s) 2648
FriOI GRGCYC 3 cut(s) 150, 1337, 1689
Fsp4HI GCNGC 1 cut(s) 2648
FspBI CTAG 9 cut(s) 38, 110, 1244, 1649, 1812, 1895, 1928, 2000, 2186
FspI TGCGCA 1 cut(s) 2750
GlaI GCGC 1 cut(s) 2750
GluI GCNGC 1 cut(s) 2648
GsaI CCCAGC 1 cut(s) 1103
HaeIII GGCC 5 cut(s) 52, 624, 720, 903, 2110
HapII CCGG 3 cut(s) 386, 620, 1696
HhaI GCGC 1 cut(s) 2751
Hin1I GRCGYC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 2749
HinP1I GCGC 1 cut(s) 2749
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HindIII AAGCTT 3 cut(s) 860, 936, 1868
HinfI GANTC 9 cut(s) 330, 551, 811, 878, 1297, 1475, 1602, 2320, 2773
HpaII CCGG 3 cut(s) 386, 620, 1696
HphI GGTGA 5 cut(s) 331, 668, 1234, 1664, 2371
Hpy166II GTNNAC 4 cut(s) 254, 1018, 1046, 1760
Hpy8I GTNNAC 4 cut(s) 254, 1018, 1046, 1760
Hpy99I CGWCG 2 cut(s) 177, 1311
HpyCH4IV ACGT 4 cut(s) 22, 232, 834, 1309
HpyF10VI GCNNNNNNNGC 2 cut(s) 1975, 2647
HpySE526I ACGT 4 cut(s) 22, 232, 834, 1309
Hsp92I GRCGYC 1 cut(s) 22
HspAI GCGC 1 cut(s) 2749
KpnI GGTACC 2 cut(s) 1064, 2094
Ksp22I TGATCA 2 cut(s) 321, 576
LguI GCTCTTC 1 cut(s) 2228
LmnI GCTCC 2 cut(s) 1332, 2575
Lsp1109I GCAGC 1 cut(s) 2634
LweI GCATC 5 cut(s) 1419, 1522, 1603, 2299, 2382
MaeI CTAG 9 cut(s) 38, 110, 1244, 1649, 1812, 1895, 1928, 2000, 2186
MaeII ACGT 4 cut(s) 22, 232, 834, 1309
MaeIII GTNAC 8 cut(s) 341, 359, 991, 1081, 1222, 2077, 2359, 2676
MfeI CAATTG 2 cut(s) 2132, 2228
MflI RGATCY 1 cut(s) 1232
MhlI GDGCHC 6 cut(s) 150, 164, 246, 1337, 1689, 2633
MlyI GAGTC 4 cut(s) 820, 887, 1291, 1611
MmeI TCCRAC 4 cut(s) 520, 737, 805, 2323
Mph1103I ATGCAT 3 cut(s) 1276, 2032, 2314
MroXI GAANNNNTTC 2 cut(s) 234, 1659
MseI TTAA 9 cut(s) 248, 498, 960, 1770, 2388, 2474, 2571, 2670, 2754
MslI CAYNNNNRTG 4 cut(s) 1625, 1963, 2213, 2349
MspA1I CMGCKG 2 cut(s) 908, 2415
MspCI CTTAAG 3 cut(s) 247, 2570, 2669
MspI CCGG 3 cut(s) 386, 620, 1696
MspR9I CCNGG 7 cut(s) 621, 1148, 1322, 1696, 1697, 2370, 2511
MunI CAATTG 2 cut(s) 2132, 2228
Mva1269I GAATGC 1 cut(s) 1316
MvaI CCWGG 4 cut(s) 1148, 1322, 2370, 2511
MwoI GCNNNNNNNGC 2 cut(s) 1975, 2647
NciI CCSGG 3 cut(s) 621, 1696, 1697
NlaIV GGNNCC 4 cut(s) 904, 1062, 1255, 2092
NmuCI GTSAC 6 cut(s) 341, 359, 1081, 1222, 2077, 2359
NsbI TGCGCA 1 cut(s) 2750
NsiI ATGCAT 3 cut(s) 1276, 2032, 2314
NspI RCATGY 5 cut(s) 412, 1166, 1777, 1978, 2218
NspV TTCGAA 1 cut(s) 1001
PaeI GCATGC 1 cut(s) 2218
PceI AGGCCT 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 2228
PctI GAATGC 1 cut(s) 1316
PdmI GAANNNNTTC 2 cut(s) 234, 1659
PfeI GAWTC 5 cut(s) 330, 551, 1475, 2320, 2773
PfoI TCCNGGA 2 cut(s) 1146, 1320
PkrI GCNGC 1 cut(s) 2649
PleI GAGTC 4 cut(s) 819, 886, 1291, 1610
PpsI GAGTC 4 cut(s) 819, 886, 1291, 1610
PsiI TTATAA 1 cut(s) 1787
Psp124BI GAGCTC 3 cut(s) 150, 1337, 1689
Psp6I CCWGG 4 cut(s) 1146, 1320, 2368, 2509
PspFI CCCAGC 1 cut(s) 1099
PspGI CCWGG 4 cut(s) 1146, 1320, 2368, 2509
PspN4I GGNNCC 4 cut(s) 904, 1062, 1255, 2092
PspPI GGNCC 6 cut(s) 180, 521, 622, 902, 1144, 1573
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
PstI CTGCAG 2 cut(s) 538, 1090
PsuI RGATCY 1 cut(s) 1232
RsaI GTAC 6 cut(s) 480, 516, 758, 1062, 1759, 2092
RsaNI GTAC 6 cut(s) 479, 515, 757, 1061, 1758, 2091
RseI CAYNNNNRTG 4 cut(s) 1625, 1963, 2213, 2349
SacI GAGCTC 3 cut(s) 150, 1337, 1689
SapI GCTCTTC 1 cut(s) 2228
SaqAI TTAA 9 cut(s) 248, 498, 960, 1770, 2388, 2474, 2571, 2670, 2754
SatI GCNGC 1 cut(s) 2648
Sau96I GGNCC 6 cut(s) 180, 521, 622, 902, 1144, 1573
SchI GAGTC 4 cut(s) 820, 887, 1291, 1611
ScrFI CCNGG 7 cut(s) 621, 1148, 1322, 1696, 1697, 2370, 2511
SduI GDGCHC 6 cut(s) 150, 164, 246, 1337, 1689, 2633
SfaNI GCATC 5 cut(s) 1419, 1522, 1603, 2299, 2382
SfcI CTRYAG 3 cut(s) 534, 1086, 1753
SfuI TTCGAA 1 cut(s) 1001
SinI GGWCC 4 cut(s) 180, 521, 1144, 1573
SmaI CCCGGG 1 cut(s) 1697
SmiMI CAYNNNNRTG 4 cut(s) 1625, 1963, 2213, 2349
SmlI CTYRAG 9 cut(s) 46, 163, 247, 1049, 1065, 1211, 2570, 2632, 2669
SmoI CTYRAG 9 cut(s) 46, 163, 247, 1049, 1065, 1211, 2570, 2632, 2669
SpeI ACTAGT 1 cut(s) 1811
SphI GCATGC 1 cut(s) 2218
SseBI AGGCCT 1 cut(s) 720
SsiI CCGC 3 cut(s) 906, 2413, 2734
SspI AATATT 2 cut(s) 1660, 2338
SspMI CTAG 9 cut(s) 38, 110, 1244, 1649, 1812, 1895, 1928, 2000, 2186
SstI GAGCTC 3 cut(s) 150, 1337, 1689
StuI AGGCCT 1 cut(s) 720
StyD4I CCNGG 7 cut(s) 619, 1146, 1320, 1694, 1695, 2368, 2509
StyI CCWWGG 1 cut(s) 1536
TaiI ACGT 4 cut(s) 25, 235, 837, 1312
TaqI TCGA 9 cut(s) 175, 333, 487, 1001, 1306, 1469, 1934, 2516, 2616
TatI WGTACW 3 cut(s) 514, 756, 1757
TfiI GAWTC 5 cut(s) 330, 551, 1475, 2320, 2773
Tru1I TTAA 9 cut(s) 248, 498, 960, 1770, 2388, 2474, 2571, 2670, 2754
Tru9I TTAA 9 cut(s) 248, 498, 960, 1770, 2388, 2474, 2571, 2670, 2754
TscAI CASTG 8 cut(s) 1035, 1090, 1382, 1425, 1627, 1883, 2351, 2633
TseFI GTSAC 6 cut(s) 341, 359, 1081, 1222, 2077, 2359
TseI GCWGC 1 cut(s) 2647
Tsp45I GTSAC 6 cut(s) 341, 359, 1081, 1222, 2077, 2359
TspMI CCCGGG 1 cut(s) 1695
TspRI CASTG 8 cut(s) 1035, 1090, 1382, 1425, 1627, 1883, 2351, 2633
Vha464I CTTAAG 3 cut(s) 247, 2570, 2669
VpaK11BI GGWCC 4 cut(s) 180, 521, 1144, 1573
XapI RAATTY 9 cut(s) 494, 968, 1105, 1172, 1546, 1854, 2008, 2039, 2059
XbaI TCTAGA 1 cut(s) 1894
XceI RCATGY 5 cut(s) 412, 1166, 1777, 1978, 2218
XcmI CCANNNNNNNNNTGG 1 cut(s) 1440
XmaI CCCGGG 1 cut(s) 1695
XmiI GTMKAC 2 cut(s) 253, 1017
XmnI GAANNNNTTC 2 cut(s) 234, 1659
XspI CTAG 9 cut(s) 38, 110, 1244, 1649, 1812, 1895, 1928, 2000, 2186
ZraI GACGTC 1 cut(s) 23
Zsp2I ATGCAT 3 cut(s) 1276, 2032, 2314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.