Rh2DG227300

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010487
Physical Location & Seq
Forward (+)
22485571 .. 22488184
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG227300.1

Sequence Viewer

Length: 1014 bp
ATGCAATGCACGAGTATCATCTCAGTGTCCACAATCCACTCCAGAAAGCTCAAACTTAGGAGGAACTCACACTCACAATTATCAGTTGCTCATCCTCAGAGCAACTTTGAAGACTATGATGATGTGAAGCTTCCTCTGCCTACCCATGTCAAATCCATAACCAGCACTTCAAACCCATTCGTCAAACACTGCCACAAGCTTCGCCTGTCTTCTTCTTATCGCCACACTCATGGTTCAGTTCTCGTTGTGGGTGCTACACCCATCAGGGAAATATGCGAGTTTCAGAAGTCATTGCAAGAGAAAACTGTTATGATGGATTGTTTGCTTCTACCGGATAATTGTGAGGTTCCCGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTCCAGTGATGAAAAAGCTTTCGGGGATGCAATCAACTGAATCTATTGAAGCAATGGCTCTAATGAGAATACCTACGAGTTTTTCAGATATCAATGCTGATGAAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTCTGGTCCTTGATGGTGTTCAGGATCCGGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCCGGGTGTTGTGATCCATTCAATGAAAAAGCACTCCGAGCAAGCCGAGGAGCATCATTCCAGCTTCCTATTATTTCTGGAACTTGGAATCATCTTGAATCCCTCATAAGTGAATTCCAAATAAAGATGCTAGCTGGCCATCCAGAGACTGATAAAAAGTCGACGCCATTTCACCTTTCTCAAAACCTTGCAGATTCTCTTGCAACAGTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTCTGTCTGAGCAATCTCGACGGCAATGTGAGCTTGTAACCATTCCAATGGCAGGAGATTTTGAGTCGCTTAATGTTTCAGTTGCTGGTGGGATTTTCTTGTATATGTTGCAACCTAAAAACCATATATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

337

Amino Acids

37.17

Weight (kDa)

6.67

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 181 - 327 3.3e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 794
AclWI GGATC 3 cut(s) 560, 573, 641
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 1 cut(s) 746
AcyI GRCGYC 1 cut(s) 797
AfaI GTAC 1 cut(s) 583
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 5 cut(s) 110, 171, 443, 655, 731
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 773
AlwI GGATC 3 cut(s) 560, 573, 641
AlwNI CAGNNNCTG 2 cut(s) 782, 962
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 1 cut(s) 769
ApoI RAATTY 1 cut(s) 746
AspS9I GGNCC 1 cut(s) 547
AsuC2I CCSGG 3 cut(s) 570, 636, 637
AsuHPI GGTGA 1 cut(s) 797
AsuNHI GCTAGC 1 cut(s) 763
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 1 cut(s) 547
BalI TGGCCA 1 cut(s) 771
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 395
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 2 cut(s) 117, 201
Bbv12I GWGCWC 1 cut(s) 395
BccI CCATC 6 cut(s) 269, 307, 535, 548, 606, 780
BceAI ACGGC 1 cut(s) 914
BcnI CCSGG 3 cut(s) 570, 636, 637
BcoDI GTCTC 1 cut(s) 773
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 2 cut(s) 511, 600
Bme1390I CCNGG 3 cut(s) 570, 636, 637
Bme18I GGWCC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 3 cut(s) 348, 524, 567
BmrFI CCNGG 3 cut(s) 570, 636, 637
BmsI GCATC 3 cut(s) 411, 695, 750
BmtI GCTAGC 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 117, 201
BpmI CTGGAG 2 cut(s) 25, 381
BpuMI CCSGG 3 cut(s) 570, 636, 637
BsaHI GRCGYC 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 635, 679
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 4 cut(s) 11, 290, 453, 908
BseDI CCNNGG 2 cut(s) 635, 679
BseGI GGATG 4 cut(s) 91, 426, 617, 772
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 4 cut(s) 11, 290, 453, 908
BseMII CTCAG 5 cut(s) 36, 110, 604, 862, 876
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 696
BshFI GGCC 1 cut(s) 771
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 332, 569, 636
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 1 cut(s) 773
BsnI GGCC 1 cut(s) 771
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 3 cut(s) 565, 593, 646
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 5 cut(s) 35, 109, 603, 861, 877
BspLI GGNNCC 3 cut(s) 348, 524, 567
BspOI GCTAGC 1 cut(s) 767
BspPI GGATC 3 cut(s) 560, 573, 641
BsrDI GCAATG 4 cut(s) 11, 290, 453, 908
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 2 cut(s) 635, 679
BssMI GATC 3 cut(s) 565, 593, 646
BssNI GRCGYC 1 cut(s) 797
BssSI CACGAG 1 cut(s) 10
Bst2BI CACGAG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 307, 512, 841
BstACI GRCGYC 1 cut(s) 797
BstC8I GCNNGC 3 cut(s) 676, 765, 769
BstDEI CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
BstF5I GGATG 4 cut(s) 91, 426, 617, 772
BstKTI GATC 3 cut(s) 568, 596, 649
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 3 cut(s) 565, 593, 646
BstMWI GCNNNNNNNGC 3 cut(s) 136, 671, 907
BstSCI CCNGG 3 cut(s) 568, 634, 635
BstSFI CTRYAG 2 cut(s) 511, 600
BstV2I GAAGAC 2 cut(s) 117, 201
BstX2I RGATCY 1 cut(s) 565
BstXI CCANNNNNNTGG 2 cut(s) 230, 925
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 1 cut(s) 771
BtsCI GGATG 4 cut(s) 91, 426, 617, 772
BtsI GCAGTG 2 cut(s) 187, 584
BtsIMutI CAGTG 5 cut(s) 30, 187, 405, 584, 846
Cac8I GCNNGC 3 cut(s) 676, 765, 769
CaiI CAGNNNCTG 2 cut(s) 782, 962
Cfr13I GGNCC 1 cut(s) 547
Cfr9I CCCGGG 1 cut(s) 635
CseI GACGC 1 cut(s) 805
Csp6I GTAC 1 cut(s) 582
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviAII CATG 2 cut(s) 146, 230
CviQI GTAC 1 cut(s) 582
DdeI CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
DpnI GATC 3 cut(s) 567, 595, 648
DpnII GATC 3 cut(s) 565, 593, 646
EaeI YGGCCR 1 cut(s) 769
Ecl136II GAGCTC 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 395
Eco32I GATATC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 746
EcoRV GATATC 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 2 cut(s) 149, 233
FatI CATG 2 cut(s) 145, 229
FblI GTMKAC 1 cut(s) 794
FokI GGATG 4 cut(s) 78, 433, 624, 759
FriOI GRGCYC 1 cut(s) 395
FspBI CTAG 1 cut(s) 764
GsuI CTGGAG 2 cut(s) 25, 381
HaeIII GGCC 1 cut(s) 771
HapII CCGG 3 cut(s) 332, 569, 636
HgaI GACGC 1 cut(s) 805
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 2 cut(s) 149, 233
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 3 cut(s) 128, 197, 410
HinfI GANTC 5 cut(s) 434, 721, 731, 827, 941
HpaII CCGG 3 cut(s) 332, 569, 636
HphI GGTGA 1 cut(s) 797
Hpy166II GTNNAC 3 cut(s) 30, 584, 795
Hpy188I TCNGA 6 cut(s) 99, 285, 481, 593, 671, 886
Hpy188III TCNNGA 7 cut(s) 42, 350, 563, 711, 728, 776, 894
Hpy8I GTNNAC 3 cut(s) 30, 584, 795
Hpy99I CGWCG 2 cut(s) 799, 900
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 3 cut(s) 307, 512, 841
HpyCH4V TGCA 8 cut(s) 4, 9, 295, 385, 424, 824, 836, 988
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 671, 907
HpyF3I CTNAG 6 cut(s) 22, 56, 96, 590, 848, 885
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 2 cut(s) 149, 233
Kzo9I GATC 3 cut(s) 565, 593, 646
LmnI GCTCC 1 cut(s) 683
LweI GCATC 3 cut(s) 411, 695, 750
MaeI CTAG 1 cut(s) 764
MaeIII GTNAC 1 cut(s) 913
MalI GATC 3 cut(s) 567, 595, 648
MboI GATC 3 cut(s) 565, 593, 646
MboII GAAGA 5 cut(s) 122, 201, 204, 620, 623
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 771
MluCI AATT 3 cut(s) 77, 337, 746
MluNI TGGCCA 1 cut(s) 771
MlyI GAGTC 1 cut(s) 950
MnlI CCTC 7 cut(s) 54, 105, 144, 337, 496, 674, 746
Mox20I TGGCCA 1 cut(s) 771
MscI TGGCCA 1 cut(s) 771
MseI TTAA 1 cut(s) 948
MslI CAYNNNNRTG 3 cut(s) 23, 228, 923
Msp20I TGGCCA 1 cut(s) 771
MspI CCGG 3 cut(s) 332, 569, 636
MspR9I CCNGG 3 cut(s) 570, 636, 637
MwoI GCNNNNNNNGC 3 cut(s) 136, 671, 907
NciI CCSGG 3 cut(s) 570, 636, 637
NdeII GATC 3 cut(s) 565, 593, 646
NheI GCTAGC 1 cut(s) 763
NlaIII CATG 2 cut(s) 149, 233
NlaIV GGNNCC 3 cut(s) 348, 524, 567
NmeAIII GCCGAG 1 cut(s) 704
PfeI GAWTC 4 cut(s) 434, 721, 731, 827
PleI GAGTC 1 cut(s) 949
PpsI GAGTC 1 cut(s) 949
Psp124BI GAGCTC 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 348, 524, 567
PspPI GGNCC 1 cut(s) 547
PstNI CAGNNNCTG 2 cut(s) 782, 962
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 1 cut(s) 583
RsaNI GTAC 1 cut(s) 582
RseI CAYNNNNRTG 3 cut(s) 23, 228, 923
SacI GAGCTC 1 cut(s) 395
SalI GTCGAC 1 cut(s) 793
SaqAI TTAA 1 cut(s) 948
Sau3AI GATC 3 cut(s) 565, 593, 646
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 1 cut(s) 950
ScrFI CCNGG 3 cut(s) 570, 636, 637
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 3 cut(s) 411, 695, 750
SfcI CTRYAG 2 cut(s) 511, 600
SinI GGWCC 1 cut(s) 547
SmaI CCCGGG 1 cut(s) 637
SmiMI CAYNNNNRTG 3 cut(s) 23, 228, 923
Sse9I AATT 3 cut(s) 77, 337, 746
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 568, 634, 635
TaaI ACNGT 3 cut(s) 307, 512, 841
TaqI TCGA 3 cut(s) 538, 794, 895
TasI AATT 3 cut(s) 77, 337, 746
TfiI GAWTC 4 cut(s) 434, 721, 731, 827
Tru1I TTAA 1 cut(s) 948
Tru9I TTAA 1 cut(s) 948
TscAI CASTG 5 cut(s) 30, 194, 405, 591, 846
TspDTI ATGAA 3 cut(s) 419, 510, 672
TspMI CCCGGG 1 cut(s) 635
TspRI CASTG 5 cut(s) 30, 194, 405, 591, 846
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 1 cut(s) 746
XmaI CCCGGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 794
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.