Rh2BG231200

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010487
Physical Location & Seq
Forward (+)
22772474 .. 22775406
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG231200.1

Sequence Viewer

Length: 948 bp
ATGGAAATACCATTGATTCATTGTTGCGTATGGAAGTTTCAATTTTTAATGCTCGGTGAATCTGTTTTTATATGTTTGTGTTTTCTGGTTCTGTGTTGCAGTCAAACTGTCAAAGTCCTCTATTTGGTTTTTGTTCTTTGTTGTCCTGCGATATATGTCGAGATAGATGAAAATGTGTTGATGAAGTTTCTTACTTTCAGGGAAATATGCGAGTTTCAGAAGTCATTGCAAGAGAAAACTGTTATGATGGATTGTTTGCTTGTACCGGATAATTGTAAGGTTCCTGAATGGATTGATGATTTATCTGTTCGTATTGTGCAAGTGAGCTCTCCTGTGATGAAAAAGCTTTCGGGGATGCAATCAACTGAATCTATTGAAGCAATGGCTCTAATGAGAATACCTACGAGTTTTTCAGATATCAATGCTGATGAAAAGGAGGCAAACTGTAGGGCTTGGTTCCCATCTCCACATCGAGTTCTGGTCCTTGATGGTGTTCAGGATCCGGGTAATCTTGGTACACTGCTCAGATCAGCTACAGCTTTTGGATGGAATGGTGCTTTTCTTCTTCCCGGGTGTTGTGATCCATTCAATGAAAAAGCACTCCGAGCAAGCCGAGGAGCATCATTCCAGCTTCCTATTATTTCTGGAACTTGGAATCATCTTGAATCCCTCATAAGTGAATTCCAAATAAAGATGCTAGCTGGCCATCCAGAGACTGAGAAAAAGTCGACGCCATTTCACCTTTCTCAAAACCTTGCAGATTCTCTTGCAACAGTGCCACTCAGCTTGGTTTTGGGTAGCGAAGGAAGTGGTCTGTCTGAGCAATCTCGACGGCAATGTGAGCTTGTAACCATTCCAATGGCAGGAGATTTTGAGTCGCTTAATGTTTCAGTTGCTGGTGGGATTTTCTTGTATATGTTGCAACCTAAAAACCATATATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

315

Amino Acids

35.02

Weight (kDa)

5.21

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 159 - 305 2.8e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 728
AclWI GGATC 3 cut(s) 494, 507, 575
AcoI YGGCCR 1 cut(s) 703
AcsI RAATTY 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 731
AfaI GTAC 2 cut(s) 264, 517
AfiI CCNNNNNNNGG 2 cut(s) 124, 863
AgsI TTSAA 4 cut(s) 41, 377, 589, 665
AluBI AGCT 8 cut(s) 327, 346, 533, 539, 631, 701, 786, 844
AluI AGCT 8 cut(s) 327, 346, 533, 539, 631, 701, 786, 844
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 707
AlwI GGATC 3 cut(s) 494, 507, 575
AlwNI CAGNNNCTG 2 cut(s) 716, 896
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 1 cut(s) 703
ApoI RAATTY 1 cut(s) 680
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 3 cut(s) 504, 570, 571
AsuHPI GGTGA 2 cut(s) 68, 731
AsuNHI GCTAGC 1 cut(s) 697
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 705
BamHI GGATCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 329
BccI CCATC 5 cut(s) 241, 469, 482, 540, 714
BceAI ACGGC 1 cut(s) 848
BcnI CCSGG 3 cut(s) 504, 570, 571
BcoDI GTCTC 1 cut(s) 707
BfaI CTAG 1 cut(s) 698
BfmI CTRYAG 2 cut(s) 445, 534
Bme1390I CCNGG 3 cut(s) 504, 570, 571
Bme18I GGWCC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 3 cut(s) 282, 458, 501
BmrFI CCNGG 3 cut(s) 504, 570, 571
BmsI GCATC 3 cut(s) 345, 629, 684
BmtI GCTAGC 1 cut(s) 701
BpuMI CCSGG 3 cut(s) 504, 570, 571
BsaHI GRCGYC 1 cut(s) 731
BsaJI CCNNGG 2 cut(s) 569, 613
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 863
Bse3DI GCAATG 3 cut(s) 224, 387, 842
BseDI CCNNGG 2 cut(s) 569, 613
BseGI GGATG 3 cut(s) 360, 551, 706
BseLI CCNNNNNNNGG 2 cut(s) 124, 863
BseMI GCAATG 3 cut(s) 224, 387, 842
BseMII CTCAG 4 cut(s) 538, 708, 796, 810
BseRI GAGGAG 1 cut(s) 630
BshFI GGCC 1 cut(s) 705
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 3 cut(s) 266, 503, 570
BslI CCNNNNNNNGG 2 cut(s) 124, 863
BsmAI GTCTC 1 cut(s) 707
BsnI GGCC 1 cut(s) 705
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 3 cut(s) 499, 527, 580
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 4 cut(s) 537, 709, 795, 811
BspLI GGNNCC 3 cut(s) 282, 458, 501
BspOI GCTAGC 1 cut(s) 701
BspPI GGATC 3 cut(s) 494, 507, 575
BsrDI GCAATG 3 cut(s) 224, 387, 842
BssECI CCNNGG 2 cut(s) 569, 613
BssMI GATC 3 cut(s) 499, 527, 580
BssNI GRCGYC 1 cut(s) 731
Bst4CI ACNGT 4 cut(s) 109, 241, 446, 775
BstACI GRCGYC 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 610, 699, 703
BstDEI CTNAG 4 cut(s) 524, 717, 782, 819
BstF5I GGATG 3 cut(s) 360, 551, 706
BstKTI GATC 3 cut(s) 502, 530, 583
BstMAI GTCTC 1 cut(s) 707
BstMBI GATC 3 cut(s) 499, 527, 580
BstMWI GCNNNNNNNGC 2 cut(s) 605, 841
BstSCI CCNGG 3 cut(s) 502, 568, 569
BstSFI CTRYAG 2 cut(s) 445, 534
BstX2I RGATCY 1 cut(s) 499
BstXI CCANNNNNNTGG 1 cut(s) 859
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 1 cut(s) 705
BtsCI GGATG 3 cut(s) 360, 551, 706
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 2 cut(s) 518, 780
Cac8I GCNNGC 3 cut(s) 610, 699, 703
CaiI CAGNNNCTG 2 cut(s) 716, 896
Cfr13I GGNCC 1 cut(s) 481
Cfr9I CCCGGG 1 cut(s) 569
CseI GACGC 1 cut(s) 739
Csp6I GTAC 2 cut(s) 263, 516
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviQI GTAC 2 cut(s) 263, 516
DdeI CTNAG 4 cut(s) 524, 717, 782, 819
DpnI GATC 3 cut(s) 501, 529, 582
DpnII GATC 3 cut(s) 499, 527, 580
EaeI YGGCCR 1 cut(s) 703
Ecl136II GAGCTC 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 329
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 481
Eco53kI GAGCTC 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 680
EcoRV GATATC 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 329
FblI GTMKAC 1 cut(s) 728
FokI GGATG 3 cut(s) 367, 558, 693
FriOI GRGCYC 1 cut(s) 329
FspBI CTAG 1 cut(s) 698
HaeIII GGCC 1 cut(s) 705
HapII CCGG 3 cut(s) 266, 503, 570
HgaI GACGC 1 cut(s) 739
Hin1I GRCGYC 1 cut(s) 731
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 7 cut(s) 16, 59, 368, 655, 665, 761, 875
HpaII CCGG 3 cut(s) 266, 503, 570
HphI GGTGA 2 cut(s) 68, 731
Hpy166II GTNNAC 2 cut(s) 518, 729
Hpy188I TCNGA 5 cut(s) 219, 415, 527, 605, 820
Hpy188III TCNNGA 7 cut(s) 160, 284, 497, 645, 662, 710, 828
Hpy8I GTNNAC 2 cut(s) 518, 729
Hpy99I CGWCG 2 cut(s) 733, 834
HpyAV CCTTC 1 cut(s) 797
HpyCH4III ACNGT 4 cut(s) 109, 241, 446, 775
HpyCH4V TGCA 7 cut(s) 99, 229, 319, 358, 758, 770, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 841
HpyF3I CTNAG 4 cut(s) 524, 717, 782, 819
Hsp92I GRCGYC 1 cut(s) 731
Kzo9I GATC 3 cut(s) 499, 527, 580
LmnI GCTCC 1 cut(s) 617
LweI GCATC 3 cut(s) 345, 629, 684
MaeI CTAG 1 cut(s) 698
MaeIII GTNAC 1 cut(s) 847
MalI GATC 3 cut(s) 501, 529, 582
MboI GATC 3 cut(s) 499, 527, 580
MboII GAAGA 2 cut(s) 554, 557
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 705
MluCI AATT 3 cut(s) 41, 271, 680
MluNI TGGCCA 1 cut(s) 705
MlyI GAGTC 1 cut(s) 884
MnlI CCTC 4 cut(s) 128, 430, 608, 680
Mox20I TGGCCA 1 cut(s) 705
MscI TGGCCA 1 cut(s) 705
MseI TTAA 2 cut(s) 47, 882
MslI CAYNNNNRTG 1 cut(s) 857
Msp20I TGGCCA 1 cut(s) 705
MspI CCGG 3 cut(s) 266, 503, 570
MspR9I CCNGG 3 cut(s) 504, 570, 571
MwoI GCNNNNNNNGC 2 cut(s) 605, 841
NciI CCSGG 3 cut(s) 504, 570, 571
NdeII GATC 3 cut(s) 499, 527, 580
NheI GCTAGC 1 cut(s) 697
NlaIV GGNNCC 3 cut(s) 282, 458, 501
NmeAIII GCCGAG 1 cut(s) 638
PfeI GAWTC 6 cut(s) 16, 59, 368, 655, 665, 761
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 282, 458, 501
PspPI GGNCC 1 cut(s) 481
PstNI CAGNNNCTG 2 cut(s) 716, 896
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 2 cut(s) 264, 517
RsaNI GTAC 2 cut(s) 263, 516
RseI CAYNNNNRTG 1 cut(s) 857
SacI GAGCTC 1 cut(s) 329
SalI GTCGAC 1 cut(s) 727
SaqAI TTAA 2 cut(s) 47, 882
Sau3AI GATC 3 cut(s) 499, 527, 580
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 3 cut(s) 504, 570, 571
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 3 cut(s) 345, 629, 684
SfcI CTRYAG 2 cut(s) 445, 534
SinI GGWCC 1 cut(s) 481
SmaI CCCGGG 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 857
Sse9I AATT 3 cut(s) 41, 271, 680
SspMI CTAG 1 cut(s) 698
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 3 cut(s) 502, 568, 569
TaaI ACNGT 4 cut(s) 109, 241, 446, 775
TaqI TCGA 4 cut(s) 159, 472, 728, 829
TasI AATT 3 cut(s) 41, 271, 680
TfiI GAWTC 6 cut(s) 16, 59, 368, 655, 665, 761
Tru1I TTAA 2 cut(s) 47, 882
Tru9I TTAA 2 cut(s) 47, 882
TscAI CASTG 2 cut(s) 525, 780
TspDTI ATGAA 6 cut(s) 8, 183, 197, 353, 444, 606
TspMI CCCGGG 1 cut(s) 569
TspRI CASTG 2 cut(s) 525, 780
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 1 cut(s) 680
XmaI CCCGGG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 728
XspI CTAG 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.