FvH4_1g21660

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007148
Physical Location & Seq
Reverse (-)
13623439 .. 13632773
9335 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21660.t2

Sequence Viewer

Length: 6333 bp
ATGGGATCAAGAGAGCGTAGTAACATGGAGGATCCAGATGGGACATTGGCAAGTGTTGCCCAATGCATTGAGCAGCTGCGTCAAAGTTCCTCATCTGTACCAGAGAAGGAGTATAACTTGAAACAGTTGCTGGAGCTTATTGGAACTCGTGAAAACGCTTTCAGTGCTGTTGGATCTCACTCTCAAGCAGTTCCTGTACTTGTTTCTCTTCTCCGATCAGGATCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTGTCTGTGTAAGGAGAATGAACTAAGGGTGAAAGTCTTGCTTGGGGGATGCATTCCACCATTGCTTGGTCTACTCAGGTCCACCTCAGCGGAGGGCCAAATTGCAGCAGCAAAGACCATCTATGCTGTTTCTCAAGGTGGTGCAAGGGATCATGTTGGATCGAAGATTTTTTCAACTGAAGGAGTTGTGCCAGTTCTTTGGGAGCTTCTACGGAAAGGGATTAAGACTGGTAGCTTGGTTGATAGCTTGTTGACCGGAGCATTAAAGAATCTATCCACGAGCACTGAAGGTTTCTGGGCTGCAACATTACAAGCTGGAGGAGTAGATATACTTGTGAAGTTGCTCACAACTGGGCAGCCAAACACTCAAGCTAATGTGTGCTTTCTTCTCGGATGTATGATGATGGAAGACGCATCTGTTTGTTCTAAGGTACTGGCTGCAGAGGCTACCAAGCAACTTCTCAAGCTTTTAGGTTCTGGCAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTCTTAAATCTCTTTCTGGCCAGTGCAAAGAAGCAAGGCGGGAGATAGCTAACTTCAATGGTATTCCTGTGTTGATAAATGCTACAATAGCTCCTTCAAAAGAATTCATGCAGGGTGAGTATGCCCAGGCATTGCAGGAAAATGCTATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTGATCTCGAGTCTTGGTCAAAGCCTTGAATCATGCACTTCACCTGCCCAAATTGCTGATACGTTAGGAGCTTTGGCTTCAGCTCTTATGATATACGATAGCAAAGCAGAATCTACAAGAGCATCGGATCCTGTTGATATTGAGCTGACTTTGGTTTCACAATTCAAGCCTAGCTTACCATTTCTTGTGCAGGAGCGAACCATAGAGGCCCTTGCAAGTTTGTATGGGAACACTGTACTCTCGGTTAAGCTCAATAATTCAGAGGCAAAACGATTGCTAGTCGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTTATGAGAGCTCTTCTAGCTCTTTGTAACAGTGAAGGAAGTCTATGGCGTGCCCTTCAAGGGCGTGAAGGGGTTCAGCTGTTGATATCACTTCTCGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTTCTTTCTAATGAGAATGATGAAAGTAAGTGGGCTATTACTGCTGCTGGTGGCATACCTCCTCTTGTTCAAATTCTAGAGACGGGATCTGCAAAAGCCAAGGAAGATTCAGCATCGATCCTTCGGAACCTATGCAATCACAGTGAAGATATACGTGCATGTGTTGAAAGTGCTGATGCAGTTCCTGCACTATTGTGGCTTCTGAAGAATGGAAGCCCAAATGGAAAAGAAATTGCAGCAAAGACTTTAAACCATTTAATCCATAAATCCGACACAGCAACCATCAGCCAACTCACTGCATTATTGACCAGTGAACTACCTGAATCCAAAGTATATGTTTTGGATGCTTTAAAAAGTATGCTGTCTGTGGTCCCTCTCAGTGATATATCGCGTGAAGGCAGTGCTGCTAATGATGCCATTGAGACGATGATAAAAATATTAAGCTCAAATAAAGAAGAGACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATTTTTGAAGCTAGAAAGGACTTGCGGGAAAGTAGTGTTGCTGTTAGAACTCTATGCTCTGCTATCAAGTTGCTTAATGTTGAATCCGGTAATATCCTTGCAGAGGCTTCACGTTGCCTGGCCGCAATATTTCTTTCCATTAAAGAGAACCGGGATGTGGCTGCTGTTGGTAGAGATGTATTATCTCCGTTGGTTGTGCTTGCTAACTCTTCGGTTCTGGAAGTGGCAGAGCCAGCAACATGTGCTTTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAAACAGCAGTGGCTGAAGACATTATTATCCCAGCTACTAGAGTTTTGCTTGAAGGTACAGTATCTGGAAAGACCCATGCAGCAGCGGCCATTGCTCGCCTGCTCCACTCTCGCCAAATTGACCATGCCTTAACTGATTGTGTGAATCGTGCCGGAACAGTTCTTGCATTAGTTTCTTTTTTAGAATCTGCTAATCATGGATCTATTGCCATATCAGAGGCACTCGAGGCACTTGCTATTCTGTCCAGGTCAGAAAGAGCAAGTGGAGAGAAAAAACCTGCCTGGGCAGTTTTAGCCGAATACCCGAAAAGCATAACGCCTATAGTTTTATCCATGGCTGATGCAACACCCTTGTTGCAGGATAAAGCCATTGAAATACTAGCAAGACTTTGCAGAGATCAACCGGTTGTACTGGGAGACACTGTTGCTACTGCCTCTAGATGTACACCATCAATTGCCAAAAGGGTGATCAATTCATCAAACTCAAAGGTCAAAGTTGGAGGTGCTGCACTCCTTATTTGTGCTGCGAAAGTTAGTCATCAGAGAGTTGTAGAAGATCTTAGTGAATCGAACTTATGTACCCATCTCATTCAATCTCTCGTTGCAATGCTTAATTTTTCGGGATATATTGGGGATGGTGAGAAGGACTCTATCAGTATTGATATACATATGAAAGAAGAACTAAAGGATGATGGATCATCTTCAAGCACAGGAGTTATTGACGGTGTGAATTTAGCTGTATGGCTACTCTCTGTTCTTGCTTGTCATGATGACAAATGCAAAATTGCCATTATGGAGTCTGGTGCTGTGGAAGTCCTCACTGACAGGATCGCATATTGTTTTTCTAATTACTCTCAGATTGATTTTAAAGAAGATAGCAGCATATGGATTTGCACTATGCTGCTAGCAATCTTGTTTCAAGACAGAGATATCATACGAGCACATGCAACCATGAAATCTATACCTGTACTTGCAAATTGGTTGAAATCAGAGGAATTAGTCGACAGATATTTTGCTGCACAAGCAATGGCTAGCCTAGTCTGTAATGGTAGCAGGGGGACTCTTCTCTCTGTTGCGAATTCCGGAGCAGCCAGCGGACTTATCTCGCTACTTGGATGTGCCGATGCTGATATTAGTGACCTTTTGGAGTTGTCAGAAGAATTTGGTTTAGTGCGTTACCCTGAGCAAGTTGCTCTAGAGAGGCTGTTCAGGGTTGAAGATATAAGGGTTGGTGCTACTTCTAGGAAAGCAATACCTTCACTTGTTGATCTTCTCAAACCAATTCCAGATCGTCCTGGAGCACCATTTTTGGCACTTGGGCTTCTGACTCAGCTTGCAAAGGATTGCTCATCAAATAAAATAGTTATGGTAGAATCAGGAGCTCTGGAAGCGCTGACCAAGTATCTTTCACTTGGCCCACAAGATGCAACAGAAGAAGCTGCTACAGATCTGTTGGGTCTGCTATTTGGTAGTGCTGAAATAAGGAAACACGAATCTGCATTTGGTGCTGTCGGTCAACTTGTGGCAGTTTTACGGTTAGGCGGAAGAGCTTCAAGGTACAGTGCTGCTAAAGCGTTGGAGAGCTTATTCTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAATCTGTCCAACCCTTGGTGGAGATTCTTAATACTGGTTCTGAGAAGGAGCAGCATGCTGCGATTGCTGCATTGGTTAGGCTGCTTAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGACGTTGAAATGAATGCAGTAGATGTTCTTTGCCGGATCCTTTCATCAAATTGTTCGATGGAGTTGAAAGGGGATGCTGCTGAGTTGTGTTGTGTTCTCTTTGGAAATACTAGAATCAGGTCAACAATGGCTGCTGCTCGATGTGTTGAGCCTCTGGTGTCTCTTCTTGTTTCCGAGTTCAGCCCTGCACAACATTCAGTTGTCCGTGCCTTGGATAAACTTGTTGATGATGAGCAACTGGGAGAACTCGTTGCAGCTCATGGTGCAGTTATTCCTCTTGTAGGTCTTCTCTATGGTAAGAATTACTTGCTTCATGAAGCTATTTCCAGAGCTCTGGTGAAATTAGGGAAAGACAGGCCTGCTTGTAAATCGGAGATGGTGAAAGCCGGTGTTATTGAGAGCATACTTGAGATCCTCCATGACGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAACTGCTCCGAATATTGACAAACAATGCGAGCATTGCTAAGGGACCTTCAGCTGCAAAAGTGGTCGAGCCACTTTTCCAGCTTCTTACAAGACCAGAATTTGGACCTGATGGGCAGCACAGTTCATTACAGGTTCTTGTTAATATTTTGGAGCATCCACAGTGTCGTTCTGATTATAGGTTGACATCCCACCAGGCTATTGAACCACTTATTCCCCTACTTGATTCTCCGGCTCCTGCAGTACAACAATTGGCAGCTGAGCTTCTATCACATTTACTCTTTGAAGAACATCTGCAAAAGGATACAGTGATACAGCAAGTGATTGGTCCTCTCATACGGGTTCTTGGCTCTGGTATACATATATTGCAGCAGAGAGCTGTGAAGGCGCTTGTCAGTATTGCTCTAGCATGGCCTAATGAAATTGCAAAAGAGGGTGGTGTCACTGAACTGTCCAGGGTGATATTGCTATCAGACCCATCTCTTCCAAATACCCTGTGGGAATCAGCTGCTTCTGTTTTATCAAGCATTCTGCAATTCAGTTCTGAATTTTATTTAGAAGTGCCGGTAGCTGTGTTGGTAAGGTTGCTTCGCTCTGGATCAGAAGGCACTGTAGTTGGTGCATTGAATGCTCTTCTGGTACTGGAAAGCGATGATGCAACCAGCGCAGAAGCAATGGCTGAAAGTGGTGCCATAGAGGCTCTTTTGGACCTTCTCAGATCTCATCAGTGTGAAGATACAGCTGCAAGGCTGTTGGAAGTCTTGTTGAATAATGTCAAGATCAGAGAAACTAAGGCTACCAAATCTGCAATATTACCATTGTCTCAGTACCTCTTAGATCCACAAACCCAAGCTCAGCAGGCGAGGTTACTAGCAACTCTAGCTCTGGGTGATTTATTCCAGAATGAGGGGCTTGCCCGAAGTACCGATGCTGTCTCAGCTTGTCGTGCTCTGGTAAATGTGCTTGAAGACCAACCTACAGAAGAAATGAAAGTTGTAGCAATATGTGCTTTGCAAAACCTTGTGATGTACAGTCGGTCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGTGTTCAGGTTGTGTTGGATCTGATTGGTTCAAGTGATCCAGATACGTCCATCCAGGCTGCCATGTTTATTAAACTTCTCTTTTCAAATCACACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCGATAACGGCTGCTATTGAAAAGGATTTATGGGCGACTGGAACTGTGAATGAGGAGTATCTGAAGGCTCTAAATTCTCTCTTTAGCAATTTCCCACGTTTGAGAGCCACAGAACCTGCAACTTTAAGCATTCCTCATCTAGTTACATCCCTCAAGACAGGGTCCGAGGCAACTCAAGAAGCTGCATTGGATGCACTCTTTCTTCTAAGGCAAGCTTGGTCAGCATGCCCAGCTGAAGTTTCTAGGGCTCAGTCAATTGCTGCTGCTGATGCGATCCCTTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGATTTCAAGAGAAAACAGAATTTTTATTACAATGTTTGCCCGGGACATTGGTGGTCATAATCAAGCGTGGAAATAATATGAAGCAGTCAGTGGGAAACCCCAGTGTATTTTGTAAACTTACACTTGGCAACACTCCACCAAGGCAAACCAAGGTTGTTTCAACCGGCCCTAATCCTGAATGGGATGAGAGCTTTTCATGGTCCTTTGAGAGTCCCCCTAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAGATGGGAAAGAGTTCATTTGGAAAGGTAACTATCCAGATCGATCGAGTAGTAATGCTGGGAGCAGTTGCGGGGGAGTACACTCTATTGCCAGAAAGCAAAAGTGGACCCTCACGGAATTTGGAAATAGAATTCCAGTGGTCTAACAAAGTAACAAGTAACGACCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2111

Amino Acids

225.22

Weight (kDa)

5.31

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 42 - 269 3.6e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 383 - 559 9.5e-10 ARMC5
C2 PF00168 1929 - 2023 3.1e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 761, 1012
Acc36I ACCTGC 4 cut(s) 761, 1012, 2482, 5713
AccB1I GGYRCC 1 cut(s) 5081
AccB7I CCANNNNNTGG 3 cut(s) 326, 3838, 4496
AccI GTMKAC 3 cut(s) 331, 3198, 4750
AccII CGCG 1 cut(s) 1816
AccIII TCCGGA 1 cut(s) 3278
AciI CCGC 8 cut(s) 350, 814, 1942, 2040, 2282, 3291, 3738, 6230
AcoI YGGCCR 3 cut(s) 793, 2037, 2283
AcuI CTGAAG 8 cut(s) 459, 567, 1023, 1649, 2232, 4428, 5672, 5844
AfeI AGCGCT 1 cut(s) 3588
AflIII ACRYGT 1 cut(s) 2156
AgeI ACCGGT 1 cut(s) 2599
AhdI GACNNNNNGTC 1 cut(s) 3827
AjnI CCWGG 8 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 4847, 5514
AjuI GAANNNNNNNTTGG 2 cut(s) 3681, 3713
AleI CACNNNNGTG 1 cut(s) 1618
Alw21I GWGCWC 9 cut(s) 545, 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
Alw26I GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
AlwNI CAGNNNCTG 8 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705
Ama87I CYCGRG 4 cut(s) 967, 1376, 2420, 5942
Aor13HI TCCGGA 1 cut(s) 3278
Aor51HI AGCGCT 1 cut(s) 3588
ArsI GACNNNNNNTTYG 2 cut(s) 3191, 3223
AsiGI ACCGGT 1 cut(s) 2599
Asp700I GAANNNNTTC 3 cut(s) 1353, 3745, 6172
AspLEI GCGC 3 cut(s) 3589, 4783, 5060
AsuC2I CCSGG 3 cut(s) 2069, 5943, 5944
AsuHPI GGTGA 9 cut(s) 301, 902, 993, 2674, 2846, 4291, 4333, 4864, 5294
AsuNHI GCTAGC 2 cut(s) 3100, 3227
AvaI CYCGRG 4 cut(s) 967, 1376, 2420, 5942
AvaII GGWCC 9 cut(s) 339, 1795, 4439, 4499, 4721, 5101, 5751, 6102, 6266
BaeGI GKGCMC 1 cut(s) 1336
BaeI ACNNNNGTAYC 2 cut(s) 2758, 2791
BalI TGGCCA 1 cut(s) 795
BamHI GGATCC 3 cut(s) 31, 1087, 3978
BanI GGYRCC 1 cut(s) 5081
BanII GRGCYC 4 cut(s) 1294, 3580, 4276, 5839
BarI GAAGNNNNNNTAC 2 cut(s) 4965, 4997
BauI CACGAG 2 cut(s) 147, 538
BbsI GAAGAC 4 cut(s) 675, 2220, 4220, 5367
Bbv12I GWGCWC 9 cut(s) 545, 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
BbvCI CCTCAGC 2 cut(s) 346, 5451
BceAI ACGGC 1 cut(s) 5613
BcgI CGANNNNNNTGC 4 cut(s) 2411, 2445, 5851, 5885
BciT130I CCWGG 8 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 4849, 5516
BciVI GTATCC 1 cut(s) 4690
BclI TGATCA 2 cut(s) 1248, 2664
BcnI CCSGG 3 cut(s) 2069, 5943, 5944
BcoDI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BfmI CTRYAG 7 cut(s) 699, 768, 2517, 3639, 4632, 5004, 5370
BfoI RGCGCY 2 cut(s) 3590, 4784
BfuAI ACCTGC 4 cut(s) 761, 1012, 2482, 5713
BfuI GTATCC 1 cut(s) 4690
BglI GCCNNNNNGGC 1 cut(s) 5090
BglII AGATCT 3 cut(s) 2752, 3643, 5111
BlpI GCTNAGC 2 cut(s) 4653, 5247
Bme18I GGWCC 9 cut(s) 339, 1795, 4439, 4499, 4721, 5101, 5751, 6102, 6266
BmeRI GACNNNNNGTC 1 cut(s) 3827
BmeT110I CYCGRG 4 cut(s) 967, 1376, 2420, 5942
BmrI ACTGGG 4 cut(s) 621, 2618, 4190, 5997
BmtI GCTAGC 2 cut(s) 3104, 3231
BmuI ACTGGG 4 cut(s) 621, 2618, 4190, 5997
BpiI GAAGAC 4 cut(s) 675, 2220, 4220, 5367
BplI GAGNNNNNCTC 2 cut(s) 2828, 2860
BpmI CTGGAG 3 cut(s) 152, 597, 3513
Bpu10I CCTNAGC 5 cut(s) 346, 3378, 3931, 4434, 5451
Bpu1102I GCTNAGC 2 cut(s) 4653, 5247
BpuEI CTTGAG 8 cut(s) 168, 378, 612, 707, 1872, 4370, 5726, 5748
BpuMI CCSGG 3 cut(s) 2069, 5943, 5944
Bsa29I ATCGAT 2 cut(s) 1541, 6201
BsaAI YACGTR 1 cut(s) 1580
BsaBI GATNNNNATC 1 cut(s) 220
BsaWI WCCGGW 4 cut(s) 515, 2003, 2599, 3278
BsaXI ACNNNNNCTCC 6 cut(s) 850, 880, 2604, 2634, 4176, 4206
Bse118I RCCGGY 4 cut(s) 2599, 4328, 4957, 6065
Bse3DI GCAATG 8 cut(s) 320, 748, 905, 2286, 2808, 3228, 4428, 5073
Bse8I GATNNNNATC 1 cut(s) 220
BseAI TCCGGA 1 cut(s) 3278
BseBI CCWGG 8 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 4849, 5516
BseCI ATCGAT 2 cut(s) 1541, 6201
BseJI GATNNNNATC 1 cut(s) 220
BseMI GCAATG 8 cut(s) 320, 748, 905, 2286, 2808, 3228, 4428, 5073
BseRI GAGGAG 3 cut(s) 594, 1476, 5657
BseSI GKGCMC 1 cut(s) 1336
BseYI CCCAGC 3 cut(s) 2227, 5818, 6217
BsgI GTGCAG 7 cut(s) 1169, 1428, 1596, 2688, 3198, 4113, 4227
Bsh1236I CGCG 1 cut(s) 1816
Bsh1285I CGRYCG 1 cut(s) 6205
BshNI GGYRCC 1 cut(s) 5081
BshTI ACCGGT 1 cut(s) 2599
BshVI ATCGAT 2 cut(s) 1541, 6201
BsiEI CGRYCG 1 cut(s) 6205
BsiHKAI GWGCWC 9 cut(s) 545, 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
BsiHKCI CYCGRG 4 cut(s) 967, 1376, 2420, 5942
BslFI GGGAC 6 cut(s) 55, 1781, 3268, 4452, 5959, 6099
BsmAI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BsmBI CGTCTC 2 cut(s) 1499, 1841
BsmFI GGGAC 6 cut(s) 55, 1781, 3268, 4452, 5959, 6099
BsmI GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
BsoBI CYCGRG 4 cut(s) 967, 1376, 2420, 5942
Bsp13I TCCGGA 1 cut(s) 3278
Bsp1407I TGTACA 2 cut(s) 2639, 5421
Bsp1720I GCTNAGC 2 cut(s) 4653, 5247
Bsp19I CCATGG 1 cut(s) 2529
BspACI CCGC 8 cut(s) 350, 814, 1942, 2040, 2282, 3291, 3738, 6230
BspDI ATCGAT 2 cut(s) 1541, 6201
BspEI TCCGGA 1 cut(s) 3278
BspFNI CGCG 1 cut(s) 1816
BspHI TCATGA 2 cut(s) 2962, 4255
BspMAI CTGCAG 3 cut(s) 703, 772, 4636
BspMI ACCTGC 4 cut(s) 761, 1012, 2482, 5713
BspOI GCTAGC 2 cut(s) 3104, 3231
BspQI GCTCTTC 3 cut(s) 1299, 3736, 5031
BspT107I GGYRCC 1 cut(s) 5081
BsrDI GCAATG 8 cut(s) 320, 748, 905, 2286, 2808, 3228, 4428, 5073
BsrFI RCCGGY 4 cut(s) 2599, 4328, 4957, 6065
BsrGI TGTACA 2 cut(s) 2639, 5421
BssAI RCCGGY 4 cut(s) 2599, 4328, 4957, 6065
BssNAI GTATAC 1 cut(s) 4751
BssSI CACGAG 2 cut(s) 147, 538
BssT1I CCWWGG 6 cut(s) 1524, 2529, 3837, 4152, 6041, 6051
Bst1107I GTATAC 1 cut(s) 4751
Bst2BI CACGAG 2 cut(s) 147, 538
Bst2UI CCWGG 8 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 4849, 5516
Bst6I CTCTTC 9 cut(s) 213, 1299, 1875, 2131, 3264, 3736, 4110, 4881, 5031
BstAPI GCANNNNNTGC 9 cut(s) 56, 767, 1610, 2159, 3701, 3815, 5021, 5399, 5780
BstAUI TGTACA 2 cut(s) 2639, 5421
BstBAI YACGTR 1 cut(s) 1580
BstDSI CCRYGG 1 cut(s) 2529
BstENI CCTNNNNNAGG 2 cut(s) 2018, 4221
BstFNI CGCG 1 cut(s) 1816
BstH2I RGCGCY 2 cut(s) 3590, 4784
BstHHI GCGC 3 cut(s) 3589, 4783, 5060
BstMAI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BstMCI CGRYCG 1 cut(s) 6205
BstNI CCWGG 8 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 4849, 5516
BstNSI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
BstSFI CTRYAG 7 cut(s) 699, 768, 2517, 3639, 4632, 5004, 5370
BstSLI GKGCMC 1 cut(s) 1336
BstUI CGCG 1 cut(s) 1816
BstV2I GAAGAC 4 cut(s) 675, 2220, 4220, 5367
BstXI CCANNNNNNTGG 2 cut(s) 459, 4276
BstZ17I GTATAC 1 cut(s) 4751
Bsu15I ATCGAT 2 cut(s) 1541, 6201
BsuI GTATCC 1 cut(s) 4690
BsuTUI ATCGAT 2 cut(s) 1541, 6201
BtgI CCRYGG 1 cut(s) 2529
BtgZI GCGATG 1 cut(s) 5058
BtsI GCAGTG 4 cut(s) 1415, 1719, 1831, 2211
BveI ACCTGC 4 cut(s) 761, 1012, 2482, 5713
CaiI CAGNNNCTG 8 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705
CciI TCATGA 2 cut(s) 2962, 4255
CfoI GCGC 3 cut(s) 3589, 4783, 5060
Cfr10I RCCGGY 4 cut(s) 2599, 4328, 4957, 6065
Cfr9I CCCGGG 1 cut(s) 5942
ClaI ATCGAT 2 cut(s) 1541, 6201
CseI GACGC 3 cut(s) 68, 680, 4373
CspAI ACCGGT 1 cut(s) 2599
CspCI CAANNNNNGTGG 2 cut(s) 6125, 6160
DraI TTTAAA 3 cut(s) 1674, 1776, 3064
DriI GACNNNNNGTC 1 cut(s) 3827
EaeI YGGCCR 3 cut(s) 793, 2037, 2283
Eam1104I CTCTTC 9 cut(s) 213, 1299, 1875, 2131, 3264, 3736, 4110, 4881, 5031
Eam1105I GACNNNNNGTC 1 cut(s) 3827
EarI CTCTTC 9 cut(s) 213, 1299, 1875, 2131, 3264, 3736, 4110, 4881, 5031
EciI GGCGGA 1 cut(s) 3753
Ecl136II GAGCTC 3 cut(s) 1292, 3578, 4274
Eco130I CCWWGG 6 cut(s) 1524, 2529, 3837, 4152, 6041, 6051
Eco147I AGGCCT 1 cut(s) 4300
Eco24I GRGCYC 4 cut(s) 1294, 3580, 4276, 5839
Eco32I GATATC 2 cut(s) 1368, 3127
Eco47I GGWCC 9 cut(s) 339, 1795, 4439, 4499, 4721, 5101, 5751, 6102, 6266
Eco47III AGCGCT 1 cut(s) 3588
Eco53kI GAGCTC 3 cut(s) 1292, 3578, 4274
Eco57I CTGAAG 8 cut(s) 459, 567, 1023, 1649, 2232, 4428, 5672, 5844
Eco88I CYCGRG 4 cut(s) 967, 1376, 2420, 5942
EcoICRI GAGCTC 3 cut(s) 1292, 3578, 4274
EcoNI CCTNNNNNAGG 2 cut(s) 2018, 4221
EcoO109I RGGNCCY 2 cut(s) 1168, 4439
EcoRI GAATTC 3 cut(s) 878, 3274, 6290
EcoRII CCWGG 8 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 4847, 5514
EcoRV GATATC 2 cut(s) 1368, 3127
EcoT14I CCWWGG 6 cut(s) 1524, 2529, 3837, 4152, 6041, 6051
EcoT22I ATGCAT 2 cut(s) 68, 314
EcoT38I GRGCYC 4 cut(s) 1294, 3580, 4276, 5839
ErhI CCWWGG 6 cut(s) 1524, 2529, 3837, 4152, 6041, 6051
Esp3I CGTCTC 2 cut(s) 1499, 1841
FalI AAGNNNNNCTT 8 cut(s) 285, 317, 1118, 1150, 4232, 4264, 5788, 5820
FaqI GGGAC 6 cut(s) 55, 1781, 3268, 4452, 5959, 6099
FauI CCCGC 3 cut(s) 807, 1935, 6223
FauNDI CATATG 3 cut(s) 958, 2865, 3080
FbaI TGATCA 2 cut(s) 1248, 2664
FblI GTMKAC 3 cut(s) 331, 3198, 4750
FriOI GRGCYC 4 cut(s) 1294, 3580, 4276, 5839
GlaI GCGC 3 cut(s) 3588, 4782, 5059
GsaI CCCAGC 3 cut(s) 2231, 5822, 6221
GsuI CTGGAG 3 cut(s) 152, 597, 3513
HaeII RGCGCY 2 cut(s) 3590, 4784
HgaI GACGC 3 cut(s) 68, 680, 4373
HhaI GCGC 3 cut(s) 3589, 4783, 5060
Hin6I GCGC 3 cut(s) 3587, 4781, 5058
HinP1I GCGC 3 cut(s) 3587, 4781, 5058
HincII GTYRAC 5 cut(s) 513, 3199, 3713, 4065, 4578
HindII GTYRAC 5 cut(s) 513, 3199, 3713, 4065, 4578
HindIII AAGCTT 3 cut(s) 725, 747, 5802
HphI GGTGA 9 cut(s) 301, 902, 993, 2674, 2846, 4291, 4333, 4864, 5294
HpyCH4IV ACGT 6 cut(s) 1022, 1579, 2029, 3945, 5507, 5686
HpySE526I ACGT 6 cut(s) 1022, 1579, 2029, 3945, 5507, 5686
HspAI GCGC 3 cut(s) 3587, 4781, 5058
Kpn2I TCCGGA 1 cut(s) 3278
Ksp22I TGATCA 2 cut(s) 1248, 2664
LguI GCTCTTC 3 cut(s) 1299, 3736, 5031
MaeII ACGT 6 cut(s) 1022, 1579, 2029, 3945, 5507, 5686
MfeI CAATTG 3 cut(s) 2649, 4643, 5844
MlsI TGGCCA 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 7 cut(s) 979, 1879, 2837, 3002, 3250, 3517, 6121
MmeI TCCRAC 9 cut(s) 151, 208, 397, 1719, 2674, 3753, 3856, 5127, 5457
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 2 cut(s) 68, 314
MroI TCCGGA 1 cut(s) 3278
MroXI GAANNNNTTC 3 cut(s) 1353, 3745, 6172
MscI TGGCCA 1 cut(s) 795
MslI CAYNNNNRTG 2 cut(s) 1618, 5121
Msp20I TGGCCA 1 cut(s) 795
MunI CAATTG 3 cut(s) 2649, 4643, 5844
Mva1269I GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
MvaI CCWGG 8 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 4849, 5516
MvnI CGCG 1 cut(s) 1816
NciI CCSGG 3 cut(s) 2069, 5943, 5944
NcoI CCATGG 1 cut(s) 2529
NdeI CATATG 3 cut(s) 958, 2865, 3080
NheI GCTAGC 2 cut(s) 3100, 3227
NmuCI GTSAC 2 cut(s) 3332, 4834
NsiI ATGCAT 2 cut(s) 68, 314
NspI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
OliI CACNNNNGTG 1 cut(s) 1618
PaeI GCATGC 2 cut(s) 3881, 5817
PaeR7I CTCGAG 2 cut(s) 967, 2420
PagI TCATGA 2 cut(s) 2962, 4255
PaqCI CACCTGC 2 cut(s) 761, 1012
PceI AGGCCT 1 cut(s) 4300
PciI ACATGT 1 cut(s) 2156
PciSI GCTCTTC 3 cut(s) 1299, 3736, 5031
PctI GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
PdmI GAANNNNTTC 3 cut(s) 1353, 3745, 6172
PflFI GACNNNGTC 1 cut(s) 5749
PflMI CCANNNNNTGG 3 cut(s) 326, 3838, 4496
PfoI TCCNGGA 1 cut(s) 3490
PinAI ACCGGT 1 cut(s) 2599
Ple19I CGATCG 1 cut(s) 6205
PleI GAGTC 7 cut(s) 978, 1879, 2837, 3001, 3250, 3517, 6120
PpsI GAGTC 7 cut(s) 978, 1879, 2837, 3001, 3250, 3517, 6120
Ppu21I YACGTR 1 cut(s) 1580
PpuMI RGGWCCY 1 cut(s) 4439
PscI ACATGT 1 cut(s) 2156
Psp124BI GAGCTC 3 cut(s) 1294, 3580, 4276
Psp5II RGGWCCY 1 cut(s) 4439
Psp6I CCWGG 8 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 4847, 5514
PspFI CCCAGC 3 cut(s) 2227, 5818, 6217
PspGI CCWGG 8 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 4847, 5514
PspPPI RGGWCCY 1 cut(s) 4439
PspXI VCTCGAGB 1 cut(s) 2420
PstI CTGCAG 3 cut(s) 703, 772, 4636
PstNI CAGNNNCTG 8 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705
PsyI GACNNNGTC 1 cut(s) 5749
PvuI CGATCG 1 cut(s) 6205
PvuII CAGCTG 7 cut(s) 76, 1360, 4448, 4652, 4901, 5135, 5822
RseI CAYNNNNRTG 2 cut(s) 1618, 5121
SacI GAGCTC 3 cut(s) 1294, 3580, 4276
SalI GTCGAC 1 cut(s) 3197
SapI GCTCTTC 3 cut(s) 1299, 3736, 5031
SchI GAGTC 7 cut(s) 979, 1879, 2837, 3002, 3250, 3517, 6121
SfcI CTRYAG 7 cut(s) 699, 768, 2517, 3639, 4632, 5004, 5370
Sfr274I CTCGAG 2 cut(s) 967, 2420
SinI GGWCC 9 cut(s) 339, 1795, 4439, 4499, 4721, 5101, 5751, 6102, 6266
SlaI CTCGAG 2 cut(s) 967, 2420
SmaI CCCGGG 1 cut(s) 5944
SmiMI CAYNNNNRTG 2 cut(s) 1618, 5121
SphI GCATGC 2 cut(s) 3881, 5817
SseBI AGGCCT 1 cut(s) 4300
SsiI CCGC 8 cut(s) 350, 814, 1942, 2040, 2282, 3291, 3738, 6230
SspI AATATT 6 cut(s) 940, 1863, 2046, 4410, 4540, 5205
SstI GAGCTC 3 cut(s) 1294, 3580, 4276
StuI AGGCCT 1 cut(s) 4300
StyI CCWWGG 6 cut(s) 1524, 2529, 3837, 4152, 6041, 6051
TaiI ACGT 6 cut(s) 1025, 1582, 2032, 3948, 5510, 5689
TaqII GACCGA 2 cut(s) 3698, 5418
TatI WGTACW 8 cut(s) 196, 1195, 2605, 2639, 3163, 4636, 5421, 6237
TauI GCSGC 2 cut(s) 2042, 2285
TseFI GTSAC 2 cut(s) 3332, 4834
Tsp45I GTSAC 2 cut(s) 3332, 4834
TspGWI ACGGA 4 cut(s) 487, 2094, 4136, 6289
TspMI CCCGGG 1 cut(s) 5942
Tth111I GACNNNGTC 1 cut(s) 5749
Van91I CCANNNNNTGG 3 cut(s) 326, 3838, 4496
VpaK11BI GGWCC 9 cut(s) 339, 1795, 4439, 4499, 4721, 5101, 5751, 6102, 6266
XagI CCTNNNNNAGG 2 cut(s) 2018, 4221
XbaI TCTAGA 3 cut(s) 1501, 2633, 3391
XceI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
XcmI CCANNNNNNNNNTGG 1 cut(s) 4887
XhoI CTCGAG 2 cut(s) 967, 2420
XmaI CCCGGG 1 cut(s) 5942
XmiI GTMKAC 3 cut(s) 331, 3198, 4750
XmnI GAANNNNTTC 3 cut(s) 1353, 3745, 6172
Zsp2I ATGCAT 2 cut(s) 68, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.