Rw2G019960

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007148
Physical Location & Seq
Reverse (-)
27075545 .. 27085038
9494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G019960.1

Sequence Viewer

Length: 6330 bp
ATGGGATCAAGAGAGCGTAGTAACATGGAGGATCCAGATGGGACATTGGCAAGTGTTGCTCAATGCATTGAGCAGCTGCGTCAGAGTTCCTCATCTGTACAAGAGAAGGAGTATTCCTTGAAACAGTTGCTGGAGCTTATTGGAACTCGTGAAAATGCTTTCAGTGCTGTTGGATCTCACTCTCAAGCAGTTCCTGTACTAGTTTCTCTTCTCCGATCAGGATCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTCTCTGTGTAAGGAGAATGAACTAAGGGTGAAAGTCTTGCTTGGGGGATGCATTCCACCATTGCTTGGTCTACTCAGGTCCACCTCAGCAGATGGCCAAATTGCAGCAGCAAAGACCATCTATGCTGTTTCTCAAGGTGGTGCAAGGGATCATGTTGGTTCGAAAATTTTTTCAACTGAAGGAGTTGTGCCAGTGCTTTGGGGGCTGCTACAGAAAGGGATCAAGACTGGTAGCTTGGTTGATAGCTTGTTGACCGGAGCATTAAAGAATCTAGCCACCAGCACTGAGGGTTTCTGGGCTGCAACATTACAAGCTGGAGGAGTGGATATACTTGTGAAGTTGCTCACAACTGGACAGCCAAACACTCAAGCTAACGTGTGCTTCCTTCTTGGATGTATGATGCTGGAAGACGCATCTGTTTGTTCTAAGGTGCTGGCTGCAGAGGCTACAAAGCAACTTCTCAAGCTTTTAAGTTCTGGCAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTCTTAAATCTCTTTCTGCCCAGTGCAAAGAAGCAAGGCGGGAGATAGCTAACTTCAATGGTATTCCTGTGTTGATAAATGCTACAATAGCCCCTTCAAAAGAATTCATGCAGGGTGAGTATGCCCAGGCATTGCAGGAAAATGCCATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTGATCTCAAGTCTTGGTCAAAGCCTTGAATCATGCACTTCACCTGCCCAAATTGCTGATACATTAGGGGCTTTGGCTTCAGCTCTTATGATATATGATAGCAAAGCAGAATCTACCAGAGCATCGGATCCTGTTGTTATTGAGCAGACTTTGGTTTCACAATTCAAGCCTACCTTACCATTTCTCGTTCAGGAGCGAACCATAGAGGCCCTTGCAAGTTTGTATGGGAATACCGTACTCTCAGTTAAACTCAATGGTTCAGAAGCAAAACGATTGCTTGTAGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTTATGAGAGCACTTCTTGCTCTTTGTAATAGTGAAGGAAGTCTATGGCGTGCCCTTCAAGGTCGCGAAGGGGTTCAGCTGTTGATATCACTTCTCGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTTCTTTCTAATGAGAATGATGAGAGTAAGTGGGCTATTACTGCTGCTGGTGGCATACCTCCTCTTGTTCAAATTCTAGAGACGGGATCTGCAAAAGCCAAGGAAGATTCAGCATCAATCCTTCGGAACCTCTGCAATCACAGCGAAGATATACGCGCATGTGTTGAAAGTGCTGATGCAGTTCCTGCACTGTTGTGGCTTCTGAAGAATGGAAGCCCAAATGGAAAAGAAATTGCAGCAAAGACTTTAAACCATTTAATCCATAAATCTGACACAGCAACCATCAGCCAACTCACTGCATTATTGACCAGTGAACTACCTGAATCTAAGGTATATGTTTTGGATGCTTTAAAAAGTATGCTGTCTGTGGTCCCTCTCAGTGATATATCGCGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATTAAAATATTAAGCTCAAATAAAGAAGAGACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATTTTTGAAGCTAGGAAGGACTTGCGGGAAAGTAGTATTGCTGTTAAAACTCTTTGGTCAGCTATCAAGTTGCTTAATGTTGAGTCAGGTAATATCCTTGTAGAGGCTTCACGTTGCCTGGCAGCAATATTTCTTTCCATTAAAGAGAACCGGGATGTGGCTGCTGTTGCTAGAGATGTATTATCTCCCTTAGTTGTTCTCGCTAATTCTTCGGTTCTGGAAGTGGCAGAGCTAGCAACATGTGCTTTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAAACAGCAGTGGCTGAAGACATTATTCTCCCAGCTACTAGAGTTTTGCTTGAAGGTACAGTATCTGGAAAGACCCATGCAGCAGCAGCAATTGCTCGCCTGCTCCACTCTCGCCAAATTGACCATGCCTTAACTGATTGTGTGAATCGTGCCGGAACAGTTCTTGCATTAGTTTCTTTTCTGGAATCTGCTAATCATGGATCTGTTGCCACATCAGGGGCACTAGATGCACTTGCTATTCTGTCCAGGTCAGAACGAGCAAGTGGAGAGAAAAAACCTGCCTGGGCAGTTTTAGCTGAATTCCCGAAAAGCATAATCCCTATAGTTTTATCCATTGCTGATGCAACACCCTTGTTGCAGGATAAAGCTATTGAAATATTATCAAGACTTTGCAGAGATCAACCTGTTGTACTGGGAGACACTGTTGCTACTGCCTCTGGATGTATACCATCAATTGCCAAAAGGGTGATCAATTCATCAAACTCAAAGGTCAAAATTGGAGGTGCTGCACTCCTTATTTGTGCTGCGAAAGTTAGTCATCAGAGAGTTCTAGAAGATCTTAGTGAATCGAACTTATGTACCCATCTCATTCAATCTCTCGTTGCGATGCTTAATTTTTCGGGAAATATAGGGGGTGGTGAGAAGGACTCTATCAGTATTGATATACATATGAAAGAAGAATTAAAGGATGATGGATCTTCTTTAAGCACAGGAGTTATTGATGGTGTGAATTTAGCTGTATGGCTACTCTCTGTTCTTGCTTGTCATGATGATAAATGCAAAATTGCCATTATGGAGGCTGGTGCTGTGGAAGTCCTCACTGACAGGATCTCATATTGTTTTTCCAATTACTCTCAGATTGATTTTAAAGAAGATAGCAGCATATGGATCTGCACTATGCTGCTAGCGATCTTGTTTCAAGACAGAGATATCATACGAGCACATGCAACCATGAAATCCATACCTGTACTTGCTAATTGGTTGAAGTCAGAGGAGTTAGTCGACAGATATTTTGCTGCACAAGCAATGGCCAGCCTAGTCTGTAATGGTAGCAGGGGGACTCTTTTATCTGTTGCAAATTCTGGAGCAGCGGTTGGACTTATCTCACTACTTGGATGTGCTGATGTTGATATTAGTGACCTATTGGATTTGTCAGAAGAATTTGCTTTAGTGCGTCACCCTGAGCAAGTTGCTCTAGAGAGGCTGTTCAGGGTTGAAGATATAAGGGTTGGTGCTACTTCTAGGAAAGCAATACCTGCACTCGTTGATCTTCTCAAACCAATCCCGGATCGTCCTGGAGCACCATTTTTGGCACTTGGGTTTCTTACTCAGCTCGCAAAGGATTGCCCTGCAAATAAAATAGTTATGGTAGAATCAGGAGCTCTGGAAGCACTGACCAAGTATCTTTCACTTGGCCCACAAGATGCAACAGAAGAAGCTGCTACAGATCTGTTGGGTCTACTATTTGGTAGTGCTGAAATAAGGAAACACGAATCTGCATTTGGTGCTGTCAATCAACTTGTGGCAGTTTTACGGTTAGGTGGAAGAGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTATTCTCTGCTGACCATATAAGGAATGCTGAAAGTGCTCGACAGTCTGTCCAACCCTTGGTGGAGATTCTTAATACTGGTGCTGAGAAGGAGCAGCATGCTGCGATTGCTGCATTGGTTAGGCTGCTTAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGACGTTGAAATGAATGCAGTGGATGTTCTTTGCAGGATCCTTTCATCAAATTGTTCGATGGAACTGAAAGGAGATGCTGCTGAGTTGTGTTGTGTTCTCTTTGGAAATACAAGAATCAGGTCAACAATGGCTGCAGCTCGATGTGTTGAGCCTCTGGTGTCTCTTCTTGTTACCGAGTTCAGCCCTGCACAACATTCAGTTGTCCGTGCCTTGGATAAACTTGTTGACGATGAACAACTGGGCGAACTCGTTGCTGCTCATGGTGCAGTTATTCCTCTTGTTGGTCTTCTCTATGGTAAGAATTACTTGCTTCATGAAGCTATTTCCAGAGCTCTGGTGAAATTAGGGAAAGACAGACCTGCTTGTAAATCGGAGATGGTGAAAGCTGGTGTTATTGAGAGCATACTTGACATCCTCCACGAAGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAATTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGCCCTTCTGCTTCAAAAGTGGTTGAGCCGCTTTTCCAGCTTCTAACAAGACCAGAATTTGGACCTGATGGGCAGCACAGTTCATTACAGGTTCTTGTCAATATTTTGGAGCATCCACAGTGTCGTTCTGATTATAGGTTGACATCCCACCAGGCTATTGAACCACTTATTCCCCTACTTGATTCTCCGGCTCCTGCAGTACAGCAATTGGCAGCTGAGCTTCTATCACATTTACTCTTTGAAGAACATCTTCAAAAGGATACAGTGATACTGCAAGTGATTGGTCCTCTCATACGGGTTCTTGGCTCTGGTATACATATATTGCAGCAGAGAGCTGTGAAAGCGCTTGTCAGTATTGCGCTAGCATGGCCGAATGAAATTGCAAAAGAAGGTGGTGTCACTGAACTGTCCAAGGTGATATTGCTGTCAGATCCATCTCTTCCAAATGCCCTGTGGGAATCAGCTGCTTCTGTTCTATCAAGCATTCTGCAATTCAGTTCTGAATTTTATTTAGAAGTGCCTGTAGCTGTGTTGGTACGGTTGCTTCGCTCTGGATCAGAAGGCACCGTAGTTGGTGCATTGAATGCTCTTTTGGTACTGGAAACTGATGATGCTACCAGCGCAGAAGCAATGGCTGAAAGTGGTGCCATAGAGGCTCTTTTGGACCTTCTAAGATCTCATCAGTGTGAAGAAACAGCTGCAAGGCTGTTGGAAGTATTGTTGAATAATGTAAAGATCAGAGAAACTAAGGCTACCAAGTCTGCCATATTACCATTGTCTCAGTACCTCTTGGATCCACAAACCCAAGCTCAGCAGGCAAGGTTACTAGCAACTCTAGCTCTTGGTGATTTATTCCAGAATGAGGGGCTTGCCCGTAGTACTGACGCTGTCTCAGCTTGTCGTGCTCTGGTAAATGTGCTTGAAGAGCAACCTACTGAAGAAATGAAAGTTGTAGCAATATGTGCTTTGCAAAACCTTGTGATGTACAGTCGGTCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGTGTTCAGGTTGTGTTGGATCTGATTGGTTCAAGTGATCCAGATACGTCCATTCAGGCTGCAATGTTTGTTAAACTTCTCTTTTCAAATCACACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCGATAACCGCTGCTATTGAAAAGGATTTATGGGCAACTGGAACTGTGAACGATGAGTATCTGAAGGCTCTGAATTCTCTTTTTAGCAATTTCCCACGTTTGAGAGCCACAGAGCCTGCAACTTTAAGCATTCCTCATCTAGTAACATCCCTCAAGACAGGGTCCGAGGCAACTCAAGAAGCTGCGTTGGATGCACTGTTTCTTCTAAGGCAAGCTTGGTCAGCATGCCCAGCTGAAGTTTCTAGGGCTCAGTCAATTGCTGCTGCTGATGCGATCCCTTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGATTTCAAGAGAAAACAGAATTTTTATTACAATGTTTGCCGGGGACATTGGTGGTCATAATCAAGCGGGGGAATAATATGAAGCAGTCAGTGGGAAACCCCAGTGTATTTTGTAAACTTACACTTGGTAACACTCCACCTAGGCAAACCCAGGTTGTTTCAACTGGCCCTAATCCTGAATGGGATGAGAGCTTTTCGTGGTCCTTTGAGAGTCCTCCTAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAGATGGGAAAGAGTTCATTTGGAAAGGTGACTATCCAGATCGATCGAGTCGTAATGCTGGGAGCAGTTGCTGGGGAGTACACACTATTGCCAGAAAGCAAAAGCGGACCCTCCCGGAATTTAGAAATAGAATTCCAGTGGTCTAACAAAGTAACAAGTAACGACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2109

Amino Acids

224.87

Weight (kDa)

5.34

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 19 - 269 2.4e-09 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 383 - 559 3.9e-09 ARMC5
C2 PF00168 1982 - 2076 6.9e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 761, 1012
Acc36I ACCTGC 5 cut(s) 761, 1012, 2482, 3460, 4309
AccB1I GGYRCC 2 cut(s) 5000, 5081
AccB7I CCANNNNNTGG 3 cut(s) 326, 3838, 4496
AccI GTMKAC 5 cut(s) 331, 2641, 3198, 3655, 4750
AccII CGCG 3 cut(s) 1347, 1581, 1816
AciI CCGC 7 cut(s) 814, 1942, 3287, 4466, 5598, 5968, 6264
AcoI YGGCCR 3 cut(s) 355, 3225, 4805
AcuI CTGAAG 7 cut(s) 459, 1023, 1649, 2232, 5394, 5672, 5844
AfeI AGCGCT 1 cut(s) 4782
AflIII ACRYGT 2 cut(s) 636, 2156
AhdI GACNNNNNGTC 1 cut(s) 3827
AhlI ACTAGT 1 cut(s) 199
AjnI CCWGG 7 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 6051
AjuI GAANNNNNNNTTGG 4 cut(s) 3681, 3713, 5012, 5044
AleI CACNNNNGTG 1 cut(s) 1618
Alw21I GWGCWC 8 cut(s) 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
Alw26I GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
AlwNI CAGNNNCTG 9 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705, 6230
Ama87I CYCGRG 1 cut(s) 1376
Aor51HI AGCGCT 1 cut(s) 4782
AoxI GGCC 9 cut(s) 355, 1167, 3225, 3610, 4438, 4805, 5893, 6067, 6124
ApaI GGGCCC 1 cut(s) 4442
ArsI GACNNNNNNTTYG 2 cut(s) 3191, 3223
Asp700I GAANNNNTTC 4 cut(s) 1353, 3745, 4686, 6172
AspA2I CCTAGG 1 cut(s) 6041
AspLEI GCGC 4 cut(s) 1583, 4783, 4798, 5060
AsuC2I CCSGG 4 cut(s) 2069, 3482, 5943, 6274
AsuII TTCGAA 1 cut(s) 422
AsuNHI GCTAGC 3 cut(s) 2149, 3100, 4798
AvaI CYCGRG 1 cut(s) 1376
AvaII GGWCC 8 cut(s) 339, 1795, 4499, 4721, 5101, 5751, 6102, 6266
AvrII CCTAGG 1 cut(s) 6041
BaeGI GKGCMC 3 cut(s) 1336, 2419, 4442
BaeI ACNNNNGTAYC 2 cut(s) 2758, 2791
BalI TGGCCA 2 cut(s) 357, 3227
BamHI GGATCC 4 cut(s) 31, 1087, 3978, 5230
BanI GGYRCC 2 cut(s) 5000, 5081
BanII GRGCYC 4 cut(s) 3580, 4276, 4442, 5839
BarI GAAGNNNNNNTAC 2 cut(s) 4965, 4997
BauI CACGAG 1 cut(s) 147
BbsI GAAGAC 3 cut(s) 675, 2220, 4220
Bbv12I GWGCWC 8 cut(s) 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
BbvCI CCTCAGC 2 cut(s) 346, 5451
BcgI CGANNNNNNTGC 4 cut(s) 4175, 4209, 5851, 5885
BciT130I CCWGG 7 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 6053
BciVI GTATCC 1 cut(s) 4690
BclI TGATCA 2 cut(s) 1248, 2664
BcnI CCSGG 4 cut(s) 2069, 3482, 5943, 6274
BcoDI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BcuI ACTAGT 1 cut(s) 199
BfmI CTRYAG 8 cut(s) 470, 699, 768, 2517, 3639, 4074, 4632, 4959
BfoI RGCGCY 1 cut(s) 4784
BfuAI ACCTGC 5 cut(s) 761, 1012, 2482, 3460, 4309
BfuI GTATCC 1 cut(s) 4690
BglI GCCNNNNNGGC 1 cut(s) 5090
BglII AGATCT 3 cut(s) 2752, 3643, 5111
BlnI CCTAGG 1 cut(s) 6041
BlpI GCTNAGC 2 cut(s) 4653, 5247
BmcAI AGTACT 1 cut(s) 5317
Bme18I GGWCC 8 cut(s) 339, 1795, 4499, 4721, 5101, 5751, 6102, 6266
BmeRI GACNNNNNGTC 1 cut(s) 3827
BmeT110I CYCGRG 1 cut(s) 1376
BmrI ACTGGG 4 cut(s) 790, 2618, 4190, 5997
BmtI GCTAGC 3 cut(s) 2153, 3104, 4802
BmuI ACTGGG 4 cut(s) 790, 2618, 4190, 5997
BpiI GAAGAC 3 cut(s) 675, 2220, 4220
BplI GAGNNNNNCTC 2 cut(s) 2828, 2860
BpmI CTGGAG 4 cut(s) 152, 597, 3300, 3513
Bpu10I CCTNAGC 5 cut(s) 346, 3378, 3931, 4434, 5451
Bpu1102I GCTNAGC 2 cut(s) 4653, 5247
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 8 cut(s) 168, 378, 612, 707, 952, 1872, 5726, 5748
BpuMI CCSGG 4 cut(s) 2069, 3482, 5943, 6274
Bsa29I ATCGAT 1 cut(s) 6201
BsaBI GATNNNNATC 2 cut(s) 220, 5646
BsaWI WCCGGW 1 cut(s) 515
BsaXI ACNNNNNCTCC 4 cut(s) 2604, 2634, 3273, 3303
Bse3DI GCAATG 8 cut(s) 320, 748, 905, 2529, 3228, 4428, 5073, 5529
Bse8I GATNNNNATC 2 cut(s) 220, 5646
BseBI CCWGG 7 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 6053
BseCI ATCGAT 1 cut(s) 6201
BseJI GATNNNNATC 2 cut(s) 220, 5646
BseMI GCAATG 8 cut(s) 320, 748, 905, 2529, 3228, 4428, 5073, 5529
BseRI GAGGAG 3 cut(s) 594, 1476, 3203
BseSI GKGCMC 3 cut(s) 1336, 2419, 4442
BseYI CCCAGC 4 cut(s) 2227, 5818, 6217, 6230
BsgI GTGCAG 8 cut(s) 1428, 1596, 2688, 3073, 3198, 3438, 4113, 4227
Bsh1236I CGCG 3 cut(s) 1347, 1581, 1816
Bsh1285I CGRYCG 1 cut(s) 6205
BshFI GGCC 9 cut(s) 357, 1169, 3227, 3612, 4440, 4807, 5895, 6069, 6126
BshNI GGYRCC 2 cut(s) 5000, 5081
BshVI ATCGAT 1 cut(s) 6201
BsiEI CGRYCG 1 cut(s) 6205
BsiHKAI GWGCWC 8 cut(s) 778, 1294, 3139, 3499, 3580, 3820, 4276, 5344
BsiHKCI CYCGRG 1 cut(s) 1376
BsiSI CCGG 7 cut(s) 516, 2068, 2349, 3482, 4625, 5942, 6274
BslFI GGGAC 4 cut(s) 55, 1781, 3268, 5959
BsmAI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BsmBI CGTCTC 1 cut(s) 1499
BsmFI GGGAC 4 cut(s) 55, 1781, 3268, 5959
BsmI GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
BsnI GGCC 9 cut(s) 357, 1169, 3227, 3612, 4440, 4807, 5895, 6069, 6126
BsoBI CYCGRG 1 cut(s) 1376
Bsp119I TTCGAA 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 4438
Bsp1407I TGTACA 2 cut(s) 97, 5421
Bsp1720I GCTNAGC 2 cut(s) 4653, 5247
Bsp68I TCGCGA 1 cut(s) 1347
BspACI CCGC 7 cut(s) 814, 1942, 3287, 4466, 5598, 5968, 6264
BspANI GGCC 9 cut(s) 357, 1169, 3227, 3612, 4440, 4807, 5895, 6069, 6126
BspDI ATCGAT 1 cut(s) 6201
BspFNI CGCG 3 cut(s) 1347, 1581, 1816
BspHI TCATGA 2 cut(s) 2962, 4255
BspMAI CTGCAG 4 cut(s) 703, 772, 4078, 4636
BspMI ACCTGC 5 cut(s) 761, 1012, 2482, 3460, 4309
BspOI GCTAGC 3 cut(s) 2153, 3104, 4802
BspQI GCTCTTC 2 cut(s) 3736, 5355
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 2 cut(s) 5000, 5081
BsrDI GCAATG 8 cut(s) 320, 748, 905, 2529, 3228, 4428, 5073, 5529
BsrGI TGTACA 2 cut(s) 97, 5421
BssNAI GTATAC 2 cut(s) 2642, 4751
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 5 cut(s) 1524, 3837, 4152, 4848, 6041
Bst1107I GTATAC 2 cut(s) 2642, 4751
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 7 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 6053
Bst6I CTCTTC 6 cut(s) 213, 1875, 3736, 4110, 4881, 5355
BstAUI TGTACA 2 cut(s) 97, 5421
BstBI TTCGAA 1 cut(s) 422
BstENI CCTNNNNNAGG 2 cut(s) 2018, 6120
BstFNI CGCG 3 cut(s) 1347, 1581, 1816
BstH2I RGCGCY 1 cut(s) 4784
BstHHI GCGC 4 cut(s) 1583, 4783, 4798, 5060
BstMAI GTCTC 8 cut(s) 1499, 1841, 1877, 2607, 4107, 5220, 5332, 5573
BstMCI CGRYCG 1 cut(s) 6205
BstNI CCWGG 7 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 6053
BstNSI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
BstSFI CTRYAG 8 cut(s) 470, 699, 768, 2517, 3639, 4074, 4632, 4959
BstSLI GKGCMC 3 cut(s) 1336, 2419, 4442
BstUI CGCG 3 cut(s) 1347, 1581, 1816
BstV2I GAAGAC 3 cut(s) 675, 2220, 4220
BstXI CCANNNNNNTGG 2 cut(s) 459, 4276
BstZ17I GTATAC 2 cut(s) 2642, 4751
Bsu15I ATCGAT 1 cut(s) 6201
BsuI GTATCC 1 cut(s) 4690
BsuRI GGCC 9 cut(s) 357, 1169, 3227, 3612, 4440, 4807, 5895, 6069, 6126
BsuTUI ATCGAT 1 cut(s) 6201
BtgZI GCGATG 1 cut(s) 2816
BtsI GCAGTG 4 cut(s) 1415, 1719, 2211, 3966
BtuMI TCGCGA 1 cut(s) 1347
BveI ACCTGC 5 cut(s) 761, 1012, 2482, 3460, 4309
CaiI CAGNNNCTG 9 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705, 6230
CciI TCATGA 2 cut(s) 2962, 4255
CfoI GCGC 4 cut(s) 1583, 4783, 4798, 5060
ClaI ATCGAT 1 cut(s) 6201
CseI GACGC 4 cut(s) 68, 680, 3359, 5330
CspCI CAANNNNNGTGG 2 cut(s) 6125, 6160
DraI TTTAAA 3 cut(s) 1674, 1776, 3064
DriI GACNNNNNGTC 1 cut(s) 3827
EaeI YGGCCR 3 cut(s) 355, 3225, 4805
Eam1104I CTCTTC 6 cut(s) 213, 1875, 3736, 4110, 4881, 5355
Eam1105I GACNNNNNGTC 1 cut(s) 3827
EarI CTCTTC 6 cut(s) 213, 1875, 3736, 4110, 4881, 5355
Ecl136II GAGCTC 2 cut(s) 3578, 4274
Eco130I CCWWGG 5 cut(s) 1524, 3837, 4152, 4848, 6041
Eco24I GRGCYC 4 cut(s) 3580, 4276, 4442, 5839
Eco32I GATATC 2 cut(s) 1368, 3127
Eco47I GGWCC 8 cut(s) 339, 1795, 4499, 4721, 5101, 5751, 6102, 6266
Eco47III AGCGCT 1 cut(s) 4782
Eco53kI GAGCTC 2 cut(s) 3578, 4274
Eco57I CTGAAG 7 cut(s) 459, 1023, 1649, 2232, 5394, 5672, 5844
Eco88I CYCGRG 1 cut(s) 1376
EcoICRI GAGCTC 2 cut(s) 3578, 4274
EcoNI CCTNNNNNAGG 2 cut(s) 2018, 6120
EcoO109I RGGNCCY 3 cut(s) 1168, 4438, 4439
EcoRI GAATTC 4 cut(s) 878, 2495, 5662, 6290
EcoRII CCWGG 7 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 6051
EcoRV GATATC 2 cut(s) 1368, 3127
EcoT14I CCWWGG 5 cut(s) 1524, 3837, 4152, 4848, 6041
EcoT22I ATGCAT 2 cut(s) 68, 314
EcoT38I GRGCYC 4 cut(s) 3580, 4276, 4442, 5839
ErhI CCWWGG 5 cut(s) 1524, 3837, 4152, 4848, 6041
Esp3I CGTCTC 1 cut(s) 1499
FaqI GGGAC 4 cut(s) 55, 1781, 3268, 5959
FauI CCCGC 3 cut(s) 807, 1935, 5961
FauNDI CATATG 3 cut(s) 958, 2865, 3080
FbaI TGATCA 2 cut(s) 1248, 2664
FblI GTMKAC 5 cut(s) 331, 2641, 3198, 3655, 4750
FriOI GRGCYC 4 cut(s) 3580, 4276, 4442, 5839
GlaI GCGC 4 cut(s) 1582, 4782, 4797, 5059
GsaI CCCAGC 4 cut(s) 2231, 5822, 6221, 6234
GsuI CTGGAG 4 cut(s) 152, 597, 3300, 3513
HaeII RGCGCY 1 cut(s) 4784
HaeIII GGCC 9 cut(s) 357, 1169, 3227, 3612, 4440, 4807, 5895, 6069, 6126
HapII CCGG 7 cut(s) 516, 2068, 2349, 3482, 4625, 5942, 6274
HgaI GACGC 4 cut(s) 68, 680, 3359, 5330
HhaI GCGC 4 cut(s) 1583, 4783, 4798, 5060
Hin6I GCGC 4 cut(s) 1581, 4781, 4796, 5058
HinP1I GCGC 4 cut(s) 1581, 4781, 4796, 5058
HincII GTYRAC 5 cut(s) 513, 3199, 4065, 4168, 4578
HindII GTYRAC 5 cut(s) 513, 3199, 4065, 4168, 4578
HindIII AAGCTT 4 cut(s) 725, 747, 3778, 5802
HpaII CCGG 7 cut(s) 516, 2068, 2349, 3482, 4625, 5942, 6274
HpyCH4IV ACGT 5 cut(s) 636, 2029, 3945, 5507, 5686
HpySE526I ACGT 5 cut(s) 636, 2029, 3945, 5507, 5686
HspAI GCGC 4 cut(s) 1581, 4781, 4796, 5058
Ksp22I TGATCA 2 cut(s) 1248, 2664
LguI GCTCTTC 2 cut(s) 3736, 5355
MaeII ACGT 5 cut(s) 636, 2029, 3945, 5507, 5686
MfeI CAATTG 5 cut(s) 1842, 2286, 2649, 4643, 5844
MlsI TGGCCA 2 cut(s) 357, 3227
MluNI TGGCCA 2 cut(s) 357, 3227
MlyI GAGTC 6 cut(s) 1879, 2009, 2837, 3250, 6121, 6216
MmeI TCCRAC 7 cut(s) 151, 208, 3271, 3856, 5127, 5457, 5757
Mox20I TGGCCA 2 cut(s) 357, 3227
Mph1103I ATGCAT 2 cut(s) 68, 314
MroXI GAANNNNTTC 4 cut(s) 1353, 3745, 4686, 6172
MscI TGGCCA 2 cut(s) 357, 3227
MslI CAYNNNNRTG 2 cut(s) 1618, 5121
Msp20I TGGCCA 2 cut(s) 357, 3227
MspA1I CMGCKG 8 cut(s) 76, 1360, 3287, 4652, 4901, 5135, 5600, 5822
MspI CCGG 7 cut(s) 516, 2068, 2349, 3482, 4625, 5942, 6274
MunI CAATTG 5 cut(s) 1842, 2286, 2649, 4643, 5844
Mva1269I GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
MvaI CCWGG 7 cut(s) 902, 2036, 2443, 2479, 3492, 4589, 6053
MvnI CGCG 3 cut(s) 1347, 1581, 1816
NciI CCSGG 4 cut(s) 2069, 3482, 5943, 6274
NdeI CATATG 3 cut(s) 958, 2865, 3080
NheI GCTAGC 3 cut(s) 2149, 3100, 4798
NmuCI GTSAC 4 cut(s) 3332, 3371, 4834, 6187
NruI TCGCGA 1 cut(s) 1347
NsiI ATGCAT 2 cut(s) 68, 314
NspI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
NspV TTCGAA 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 1618
PaeI GCATGC 2 cut(s) 3881, 5817
PagI TCATGA 2 cut(s) 2962, 4255
PaqCI CACCTGC 2 cut(s) 761, 1012
PciI ACATGT 1 cut(s) 2156
PciSI GCTCTTC 2 cut(s) 3736, 5355
PcsI WCGNNNNNNNCGW 2 cut(s) 4368, 6207
PctI GAATGC 6 cut(s) 312, 3811, 3961, 4920, 5026, 5718
PdmI GAANNNNTTC 4 cut(s) 1353, 3745, 4686, 6172
PflFI GACNNNGTC 2 cut(s) 5324, 5749
PflMI CCANNNNNTGG 3 cut(s) 326, 3838, 4496
PfoI TCCNGGA 3 cut(s) 3480, 3490, 6272
Ple19I CGATCG 1 cut(s) 6205
PleI GAGTC 6 cut(s) 1879, 2008, 2837, 3250, 6120, 6215
PpsI GAGTC 6 cut(s) 1879, 2008, 2837, 3250, 6120, 6215
PscI ACATGT 1 cut(s) 2156
Psp124BI GAGCTC 2 cut(s) 3580, 4276
Psp6I CCWGG 7 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 6051
PspFI CCCAGC 4 cut(s) 2227, 5818, 6217, 6230
PspGI CCWGG 7 cut(s) 900, 2034, 2441, 2477, 3490, 4587, 6051
PspOMI GGGCCC 1 cut(s) 4438
PstI CTGCAG 4 cut(s) 703, 772, 4078, 4636
PstNI CAGNNNCTG 9 cut(s) 130, 194, 767, 1610, 2210, 2261, 5450, 5705, 6230
PsyI GACNNNGTC 2 cut(s) 5324, 5749
PvuI CGATCG 1 cut(s) 6205
PvuII CAGCTG 6 cut(s) 76, 1360, 4652, 4901, 5135, 5822
RruI TCGCGA 1 cut(s) 1347
RseI CAYNNNNRTG 2 cut(s) 1618, 5121
SacI GAGCTC 2 cut(s) 3580, 4276
SalI GTCGAC 1 cut(s) 3197
SapI GCTCTTC 2 cut(s) 3736, 5355
ScaI AGTACT 1 cut(s) 5317
SchI GAGTC 6 cut(s) 1879, 2009, 2837, 3250, 6121, 6216
SfcI CTRYAG 8 cut(s) 470, 699, 768, 2517, 3639, 4074, 4632, 4959
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 8 cut(s) 339, 1795, 4499, 4721, 5101, 5751, 6102, 6266
SmiMI CAYNNNNRTG 2 cut(s) 1618, 5121
SmlI CTYRAG 8 cut(s) 183, 393, 627, 722, 967, 1887, 5741, 5763
SmoI CTYRAG 8 cut(s) 183, 393, 627, 722, 967, 1887, 5741, 5763
SpeI ACTAGT 1 cut(s) 199
SphI GCATGC 2 cut(s) 3881, 5817
SsiI CCGC 7 cut(s) 814, 1942, 3287, 4466, 5598, 5968, 6264
SspI AATATT 6 cut(s) 940, 1863, 2046, 2574, 4410, 4540
SstI GAGCTC 2 cut(s) 3580, 4276
StyI CCWWGG 5 cut(s) 1524, 3837, 4152, 4848, 6041
TaiI ACGT 5 cut(s) 639, 2032, 3948, 5510, 5689
TaqI TCGA 9 cut(s) 422, 2765, 3198, 3820, 3998, 4081, 5902, 6201, 6205
TaqII GACCGA 1 cut(s) 5418
TatI WGTACW 8 cut(s) 97, 196, 2605, 3163, 4636, 5315, 5421, 6237
TauI GCSGC 1 cut(s) 4468
TseFI GTSAC 4 cut(s) 3332, 3371, 4834, 6187
Tsp45I GTSAC 4 cut(s) 3332, 3371, 4834, 6187
TspGWI ACGGA 1 cut(s) 4136
Tth111I GACNNNGTC 2 cut(s) 5324, 5749
Van91I CCANNNNNTGG 3 cut(s) 326, 3838, 4496
VpaK11BI GGWCC 8 cut(s) 339, 1795, 4499, 4721, 5101, 5751, 6102, 6266
XagI CCTNNNNNAGG 2 cut(s) 2018, 6120
XbaI TCTAGA 3 cut(s) 1501, 2746, 3391
XceI RCATGY 5 cut(s) 1587, 2160, 3143, 3881, 5817
XcmI CCANNNNNNNNNTGG 1 cut(s) 4887
XmaJI CCTAGG 1 cut(s) 6041
XmiI GTMKAC 5 cut(s) 331, 2641, 3198, 3655, 4750
XmnI GAANNNNTTC 4 cut(s) 1353, 3745, 4686, 6172
ZrmI AGTACT 1 cut(s) 5317
Zsp2I ATGCAT 2 cut(s) 68, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.