pycom15g29630

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007148
Physical Location & Seq
Forward (+)
26550930 .. 26553536
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29630.1

Sequence Viewer

Length: 2607 bp
ATGCAGATTGAGTTTAAAGAAGATAGTAGCATATGGATTTGTACTTTGCTGCTGGCAATCTTGTTTCAAAACAGAGACATCATACGAGCACATGCAACCATGAAATCTATACCTGTACTTGCAAACTGGTTGAGATCAGAGGAGATGCCCACCAGATATTTTGCTGCACAGGCAATGGCCAGCCTTGTCTGTAATGGTAGCAGGGGGACTCTTCTATCTGTTGCAAATTCTGGAGCAGCAGGCGGGCTCATTTCCCTACTTGGCTGTGCGGATGTTGATATAAGTGATCTTTTGCAGTTGTCAGAAGAGTTTGGTTTAGTGCGTTATCCTGAGCAAGTTGCACTCGAGAAGTTGTTCAGAGTTGAAGACATAAGGGTTGGTGCCACTTCTAGGAAAGCAATACCTGCACTTGTTGATCTTCTCAAACCCATTCCAGATCGTCCTGGAGCACCATTTTTAGCACTCGGGCTTCTGACTCAGCTTGCAAAAGACTGTCCATCAAATAAAATTGTAATGGTAGAATCAGGAGCTTTGGAAGCACTGACCAAGTATCTTTCACTTGGCCCTCAAGATGCAACTGAGGAAGCTGCTACAGATCTGTTAGGTATACTATTTGGCAGTGCTGAAATTAGGAGACATGACTCTTCATTTGGTGCTGTTGGTCAACTTGTGGCAGTTTTACGGTTAGGTGGAAGGGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTATTTTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAAGCTGTTCAACCCTTGGTGGAGATTCTCAATACTGGTTCTGAGAAGGAGCAGCATGCTGCAATTGCTGCGTTGGTAAGGCTGCTGAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGACGTTGAAATGAATGCAGTGGATGTTCTTTGCAAGATTCTTTCATCAAATTGCTCAATGGAGCTGAAAGGGGATGCTGCCGAGTTGTGTTGTGTTCTCTTTGGGAATACAAGAATCAGGTCAACAATGGCTGCGGCTCGATGTGTGGAGCCTCTGGTGTCTCTTCTTGTGACTGAGTTCAGTCCTGCGCAGCATTCAGTTGTCCGTGCGTTGGATAAACTTGTTGACGATGAGCAACTGGCAGAACTTGTCGCTGCTCACGGTGCAGTTATTCCTCTTGTAGGTCTTCTCTATGGTAAGAATTACCTGCTCCATGAAGCTATTTCCAGAGCCCTTGTGAAATTAGGGAAAGACAGGCCTGCTTGTAAGATGGAAATGGTGAAAGCTGGAGTGATTGAGAGCATACTTGACATCCTCCATGAGGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAACTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGACCATCTGCTTCAAAAGTGGTTGAGCCTCTTTTTGTGCTGCTAACAAGACCAGAGTTTGGGCCTGATGGGCAGCATAGTGCACTACAAGTTCTTGTCAACATTTTGGAGCATCCACAGTGTCGTTCTGACTATAAGTTGACATCCCACCAAGCTATTGAACCTATTATTCCCTTACTTGATTCTCCTGCACCAGCAGTACAACAGTTGGCCGCAGAGCTTCTGTCACATTTACTCTTTGAAGAGCAACTTCAAAAGGATTCAGTGACTCAGCAAGTGATTGGTCCTCTTATACGGGTTCTTGGCTCTGGTATACACATATTGCAGCAGAGAGCTGTGAAGGCCCTTGTTAGTATTGCGCTAATATGGCCGAATGAAATAGCAAAAGAGGGTGGTGTCACTGAACTGTCCAAGGTGATATTGCAATCAGATCCATCTCTTCCTCATGCCTTGTGGGAATCGGCTGCTGCTGTTTTATCAAGCATTCTGCAGTTTAGTTCTGAATTTTATTTGGAAGTGCCTGTTGCTGTTTTGGTAAGGTTGCTTCGTTCTGGCACAGAAGGCACAGTAATTGGTGCATTGAATGCTCTTCTGGTACTGGAAAGTGACGACGCAACAAGCGCAGAAGCAATGGCTGAAAGTGGTGCTTTAGAGGCTCTTTTAGAACTTCTCAGATCTCATCAGTGTGAGGAAACTGCTGCAAGGCTTTTGGAAGTATTGTTGAACAATGTGAAGATCAGAGAAACTAAGGCTACCAAGTCTGCCATATTACCGTTGTCTCAGTACCTCTTGGATCCACAAACCCAAGCCCAGCAAGCAAGGTTGCTAGCAACTCTAGCTCTTGGTGATCTATTCCAGAACGAGGGCCTTGCTCGAAGTGCTGATGCTGTTTCAGCTTGTCGTGCACTAGTAAATGTGCTTGAAGACCAACCTACTGAAGAAATGAAGGTTGTAGCGATATGTGCTTTGCAAAACCTTGTCATGTACAGTAGATCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGCGTCCAGGTTGTGTTGGATCTGATTGGTTCAAGTGATCCAGATACATCTATTCAGGCTGCAATGTTTGTTAAACTTCTGTTTTCAAACCATACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCCATTACTGGTAAGTTAATTTTGGCTTTTACGCAGAATTTTATTCAGCTATCTAAGGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

869

Amino Acids

93.42

Weight (kDa)

5.22

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 366 - 527 6.5e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1083
Acc36I ACCTGC 2 cut(s) 412, 1209
AccB1I GGYRCC 2 cut(s) 380, 1318
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccI GTMKAC 2 cut(s) 607, 1702
AciI CCGC 4 cut(s) 243, 269, 1028, 1602
AclWI GGATC 5 cut(s) 1814, 2177, 2190, 2439, 2444
AcoI YGGCCR 3 cut(s) 177, 1599, 1757
AcsI RAATTY 3 cut(s) 226, 1892, 2578
AcuI CTGAAG 1 cut(s) 2346
AcyI GRCGYC 1 cut(s) 2415
AfaI GTAC 7 cut(s) 43, 117, 707, 1590, 1986, 2174, 2375
AfiI CCNNNNNNNGG 6 cut(s) 390, 1105, 1175, 1315, 1448, 2251
AhlI ACTAGT 1 cut(s) 2296
AjnI CCWGG 2 cut(s) 442, 2418
Alw21I GWGCWC 5 cut(s) 91, 451, 772, 1474, 2296
Alw26I GTCTC 5 cut(s) 69, 628, 1059, 2172, 2525
Alw44I GTGCAC 2 cut(s) 1470, 2292
AlwI GGATC 5 cut(s) 1814, 2177, 2190, 2439, 2444
AlwNI CAGNNNCTG 1 cut(s) 2402
Ama87I CYCGRG 2 cut(s) 344, 464
AoxI GGCC 9 cut(s) 177, 562, 1250, 1451, 1599, 1731, 1757, 2254, 2541
ApaLI GTGCAC 2 cut(s) 1470, 2292
ApoI RAATTY 3 cut(s) 226, 1892, 2578
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
Asp700I GAANNNNTTC 2 cut(s) 353, 697
AspLEI GCGC 3 cut(s) 1084, 1750, 2012
AspS9I GGNCC 7 cut(s) 563, 1391, 1451, 1673, 1732, 2254, 2541
AsuHPI GGTGA 3 cut(s) 1285, 1816, 2246
AsuNHI GCTAGC 1 cut(s) 2215
AvaI CYCGRG 2 cut(s) 344, 464
AvaII GGWCC 2 cut(s) 1391, 1673
BaeGI GKGCMC 2 cut(s) 1474, 2296
BalI TGGCCA 1 cut(s) 179
BamHI GGATCC 1 cut(s) 2182
BanI GGYRCC 2 cut(s) 380, 1318
BanII GRGCYC 2 cut(s) 249, 1228
BarI GAAGNNNNNNTAC 2 cut(s) 1917, 1949
BbsI GAAGAC 3 cut(s) 372, 1172, 2319
Bbv12I GWGCWC 5 cut(s) 91, 451, 772, 1474, 2296
BbvCI CCTCAGC 1 cut(s) 2403
BccI CCATC 6 cut(s) 505, 880, 1258, 1402, 1451, 1831
BcgI CGANNNNNNTGC 2 cut(s) 2240, 2274
BciT130I CCWGG 2 cut(s) 444, 2420
BcoDI GTCTC 5 cut(s) 69, 628, 1059, 2172, 2525
BcuI ACTAGT 1 cut(s) 2296
BfaI CTAG 5 cut(s) 390, 2216, 2225, 2297, 2526
BfmI CTRYAG 2 cut(s) 591, 1877
BfuAI ACCTGC 2 cut(s) 412, 1209
BglI GCCNNNNNGGC 1 cut(s) 1459
BglII AGATCT 3 cut(s) 595, 2063, 2381
Bme1390I CCNGG 2 cut(s) 444, 2420
Bme18I GGWCC 2 cut(s) 1391, 1673
BmeT110I CYCGRG 2 cut(s) 344, 464
BmgT120I GGNCC 7 cut(s) 563, 1391, 1451, 1673, 1732, 2254, 2541
BmiI GGNNCC 5 cut(s) 382, 1044, 1320, 1392, 2184
BmrFI CCNGG 2 cut(s) 444, 2420
BmsI GCATC 5 cut(s) 135, 562, 958, 1510, 2263
BmtI GCTAGC 1 cut(s) 2219
BpiI GAAGAC 3 cut(s) 372, 1172, 2319
BpmI CTGGAG 3 cut(s) 252, 465, 1302
Bpu10I CCTNAGC 4 cut(s) 330, 883, 1386, 2403
BpuEI CTTGAG 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 2415
BsaJI CCNNGG 2 cut(s) 789, 1800
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 1105, 1175, 1315, 1448, 2251
Bse1I ACTGG 5 cut(s) 131, 814, 1137, 1992, 2554
Bse3DI GCAATG 4 cut(s) 180, 1380, 2025, 2481
BseBI CCWGG 2 cut(s) 444, 2420
BseDI CCNNGG 2 cut(s) 789, 1800
BseGI GGATG 6 cut(s) 277, 922, 973, 1305, 1501, 1532
BseLI CCNNNNNNNGG 6 cut(s) 390, 1105, 1175, 1315, 1448, 2251
BseMI GCAATG 4 cut(s) 180, 1380, 2025, 2481
BseNI ACTGG 5 cut(s) 131, 814, 1137, 1992, 2554
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 2 cut(s) 1474, 2296
BseYI CCCAGC 1 cut(s) 2199
BsgI GTGCAG 4 cut(s) 150, 390, 1179, 1563
BshFI GGCC 9 cut(s) 179, 564, 1252, 1453, 1601, 1733, 1759, 2256, 2543
BshNI GGYRCC 2 cut(s) 380, 1318
BsiHKAI GWGCWC 5 cut(s) 91, 451, 772, 1474, 2296
BsiHKCI CYCGRG 2 cut(s) 344, 464
BslFI GGGAC 2 cut(s) 220, 1404
BslI CCNNNNNNNGG 6 cut(s) 390, 1105, 1175, 1315, 1448, 2251
BsmAI GTCTC 5 cut(s) 69, 628, 1059, 2172, 2525
BsmFI GGGAC 2 cut(s) 220, 1404
BsmI GAATGC 5 cut(s) 763, 913, 1087, 1872, 1978
BsnI GGCC 9 cut(s) 179, 564, 1252, 1453, 1601, 1733, 1759, 2256, 2543
BsoBI CYCGRG 2 cut(s) 344, 464
Bsp1286I GDGCHC 7 cut(s) 91, 249, 451, 772, 1228, 1474, 2296
Bsp1407I TGTACA 1 cut(s) 2373
BspACI CCGC 4 cut(s) 243, 269, 1028, 1602
BspANI GGCC 9 cut(s) 179, 564, 1252, 1453, 1601, 1733, 1759, 2256, 2543
BspLI GGNNCC 5 cut(s) 382, 1044, 1320, 1392, 2184
BspMAI CTGCAG 1 cut(s) 1881
BspMI ACCTGC 2 cut(s) 412, 1209
BspOI GCTAGC 1 cut(s) 2219
BspPI GGATC 5 cut(s) 1814, 2177, 2190, 2439, 2444
BspQI GCTCTTC 2 cut(s) 1626, 1983
BspT107I GGYRCC 2 cut(s) 380, 1318
BsrDI GCAATG 4 cut(s) 180, 1380, 2025, 2481
BsrGI TGTACA 1 cut(s) 2373
BsrI ACTGG 5 cut(s) 131, 814, 1137, 1992, 2554
BssECI CCNNGG 2 cut(s) 789, 1800
BssNAI GTATAC 2 cut(s) 608, 1703
BssNI GRCGYC 1 cut(s) 2415
BssT1I CCWWGG 2 cut(s) 789, 1800
Bst1107I GTATAC 2 cut(s) 608, 1703
Bst2UI CCWGG 2 cut(s) 444, 2420
Bst6I CTCTTC 7 cut(s) 216, 300, 649, 1062, 1626, 1833, 1983
BstACI GRCGYC 1 cut(s) 2415
BstAPI GCANNNNNTGC 5 cut(s) 404, 767, 842, 1382, 1973
BstAUI TGTACA 1 cut(s) 2373
BstENI CCTNNNNNAGG 2 cut(s) 1173, 1313
BstF5I GGATG 6 cut(s) 277, 922, 973, 1305, 1501, 1532
BstHHI GCGC 3 cut(s) 1084, 1750, 2012
BstMAI GTCTC 5 cut(s) 69, 628, 1059, 2172, 2525
BstNI CCWGG 2 cut(s) 444, 2420
BstNSI RCATGY 2 cut(s) 95, 833
BstSCI CCNGG 2 cut(s) 442, 2418
BstSFI CTRYAG 2 cut(s) 591, 1877
BstSLI GKGCMC 2 cut(s) 1474, 2296
BstV2I GAAGAC 3 cut(s) 372, 1172, 2319
BstX2I RGATCY 6 cut(s) 595, 1819, 2063, 2182, 2381, 2431
BstYI RGATCY 6 cut(s) 595, 1819, 2063, 2182, 2381, 2431
BstZ17I GTATAC 2 cut(s) 608, 1703
BsuRI GGCC 9 cut(s) 179, 564, 1252, 1453, 1601, 1733, 1759, 2256, 2543
BtsCI GGATG 6 cut(s) 277, 922, 973, 1305, 1501, 1532
BtsI GCAGTG 2 cut(s) 625, 918
BtsIMutI CAGTG 8 cut(s) 539, 625, 715, 918, 1514, 1659, 1788, 2078
BveI ACCTGC 2 cut(s) 412, 1209
CaiI CAGNNNCTG 1 cut(s) 2402
CfoI GCGC 3 cut(s) 1084, 1750, 2012
Cfr13I GGNCC 7 cut(s) 563, 1391, 1451, 1673, 1732, 2254, 2541
CseI GACGC 2 cut(s) 2009, 2404
Csp6I GTAC 7 cut(s) 42, 116, 706, 1589, 1985, 2173, 2374
CviAII CATG 8 cut(s) 92, 100, 638, 830, 1208, 1313, 1835, 2371
CviQI GTAC 7 cut(s) 42, 116, 706, 1589, 1985, 2173, 2374
DraI TTTAAA 1 cut(s) 16
EaeI YGGCCR 3 cut(s) 177, 1599, 1757
Eam1104I CTCTTC 7 cut(s) 216, 300, 649, 1062, 1626, 1833, 1983
EarI CTCTTC 7 cut(s) 216, 300, 649, 1062, 1626, 1833, 1983
Eco130I CCWWGG 2 cut(s) 789, 1800
Eco147I AGGCCT 1 cut(s) 1252
Eco24I GRGCYC 2 cut(s) 249, 1228
Eco47I GGWCC 2 cut(s) 1391, 1673
Eco57I CTGAAG 1 cut(s) 2346
Eco88I CYCGRG 2 cut(s) 344, 464
EcoNI CCTNNNNNAGG 2 cut(s) 1173, 1313
EcoO109I RGGNCCY 2 cut(s) 1732, 2254
EcoRII CCWGG 2 cut(s) 442, 2418
EcoT14I CCWWGG 2 cut(s) 789, 1800
EcoT38I GRGCYC 2 cut(s) 249, 1228
ErhI CCWWGG 2 cut(s) 789, 1800
FaeI CATG 8 cut(s) 95, 103, 641, 833, 1211, 1316, 1838, 2374
FalI AAGNNNNNCTT 4 cut(s) 1623, 1655, 2020, 2052
FaqI GGGAC 2 cut(s) 220, 1404
FatI CATG 8 cut(s) 91, 99, 637, 829, 1207, 1312, 1834, 2370
FauI CCCGC 1 cut(s) 236
FauNDI CATATG 1 cut(s) 32
FblI GTMKAC 2 cut(s) 607, 1702
FokI GGATG 6 cut(s) 284, 929, 980, 1292, 1488, 1519
FriOI GRGCYC 2 cut(s) 249, 1228
FspBI CTAG 5 cut(s) 390, 2216, 2225, 2297, 2526
FspI TGCGCA 1 cut(s) 1083
GlaI GCGC 3 cut(s) 1083, 1749, 2011
GsaI CCCAGC 1 cut(s) 2203
GsuI CTGGAG 3 cut(s) 252, 465, 1302
HaeIII GGCC 9 cut(s) 179, 564, 1252, 1453, 1601, 1733, 1759, 2256, 2543
HgaI GACGC 2 cut(s) 2009, 2404
HhaI GCGC 3 cut(s) 1084, 1750, 2012
Hin1I GRCGYC 1 cut(s) 2415
Hin1II CATG 8 cut(s) 95, 103, 641, 833, 1211, 1316, 1838, 2374
Hin6I GCGC 3 cut(s) 1082, 1748, 2010
HinP1I GCGC 3 cut(s) 1082, 1748, 2010
HincII GTYRAC 5 cut(s) 665, 1017, 1120, 1489, 1530
HindII GTYRAC 5 cut(s) 665, 1017, 1120, 1489, 1530
HindIII AAGCTT 1 cut(s) 730
HphI GGTGA 3 cut(s) 1285, 1816, 2246
Hpy166II GTNNAC 9 cut(s) 608, 665, 1017, 1120, 1472, 1489, 1530, 1703, 2294
Hpy8I GTNNAC 9 cut(s) 608, 665, 1017, 1120, 1472, 1489, 1530, 1703, 2294
Hpy99I CGWCG 1 cut(s) 2003
HpyAV CCTTC 5 cut(s) 687, 814, 1723, 1943, 2329
HpyCH4IV ACGT 1 cut(s) 897
HpySE526I ACGT 1 cut(s) 897
Hsp92I GRCGYC 1 cut(s) 2415
Hsp92II CATG 8 cut(s) 95, 103, 641, 833, 1211, 1316, 1838, 2374
HspAI GCGC 3 cut(s) 1082, 1748, 2010
LguI GCTCTTC 2 cut(s) 1626, 1983
LweI GCATC 5 cut(s) 135, 562, 958, 1510, 2263
MaeI CTAG 5 cut(s) 390, 2216, 2225, 2297, 2526
MaeII ACGT 1 cut(s) 897
MaeIII GTNAC 5 cut(s) 1063, 1614, 1654, 1786, 1994
MfeI CAATTG 1 cut(s) 837
MflI RGATCY 6 cut(s) 595, 1819, 2063, 2182, 2381, 2431
MhlI GDGCHC 7 cut(s) 91, 249, 451, 772, 1228, 1474, 2296
MlsI TGGCCA 1 cut(s) 179
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 4 cut(s) 202, 469, 635, 1651
MmeI TCCRAC 2 cut(s) 1086, 2409
Mox20I TGGCCA 1 cut(s) 179
MroXI GAANNNNTTC 2 cut(s) 353, 697
MscI TGGCCA 1 cut(s) 179
MseI TTAA 3 cut(s) 15, 2484, 2558
MslI CAYNNNNRTG 1 cut(s) 2073
Msp20I TGGCCA 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 444, 2420
MunI CAATTG 1 cut(s) 837
Mva1269I GAATGC 5 cut(s) 763, 913, 1087, 1872, 1978
MvaI CCWGG 2 cut(s) 444, 2420
NdeI CATATG 1 cut(s) 32
NheI GCTAGC 1 cut(s) 2215
NlaIII CATG 8 cut(s) 95, 103, 641, 833, 1211, 1316, 1838, 2374
NlaIV GGNNCC 5 cut(s) 382, 1044, 1320, 1392, 2184
NmeAIII GCCGAG 1 cut(s) 1000
NmuCI GTSAC 5 cut(s) 1063, 1614, 1654, 1786, 1994
NsbI TGCGCA 1 cut(s) 1083
NspI RCATGY 2 cut(s) 95, 833
PaeI GCATGC 1 cut(s) 833
PaeR7I CTCGAG 1 cut(s) 344
PceI AGGCCT 1 cut(s) 1252
PciSI GCTCTTC 2 cut(s) 1626, 1983
PctI GAATGC 5 cut(s) 763, 913, 1087, 1872, 1978
PdmI GAANNNNTTC 2 cut(s) 353, 697
PfeI GAWTC 7 cut(s) 521, 799, 931, 1008, 1571, 1649, 1847
PflMI CCANNNNNTGG 1 cut(s) 1448
PfoI TCCNGGA 1 cut(s) 442
PleI GAGTC 4 cut(s) 202, 469, 635, 1651
PpsI GAGTC 4 cut(s) 202, 469, 635, 1651
Psp6I CCWGG 2 cut(s) 442, 2418
PspFI CCCAGC 1 cut(s) 2199
PspGI CCWGG 2 cut(s) 442, 2418
PspN4I GGNNCC 5 cut(s) 382, 1044, 1320, 1392, 2184
PspPI GGNCC 7 cut(s) 563, 1391, 1451, 1673, 1732, 2254, 2541
PstI CTGCAG 1 cut(s) 1881
PstNI CAGNNNCTG 1 cut(s) 2402
PsuI RGATCY 6 cut(s) 595, 1819, 2063, 2182, 2381, 2431
RsaI GTAC 7 cut(s) 43, 117, 707, 1590, 1986, 2174, 2375
RsaNI GTAC 7 cut(s) 42, 116, 706, 1589, 1985, 2173, 2374
RseI CAYNNNNRTG 1 cut(s) 2073
SapI GCTCTTC 2 cut(s) 1626, 1983
SaqAI TTAA 3 cut(s) 15, 2484, 2558
Sau96I GGNCC 7 cut(s) 563, 1391, 1451, 1673, 1732, 2254, 2541
SchI GAGTC 4 cut(s) 202, 469, 635, 1651
ScrFI CCNGG 2 cut(s) 444, 2420
SduI GDGCHC 7 cut(s) 91, 249, 451, 772, 1228, 1474, 2296
SfaNI GCATC 5 cut(s) 135, 562, 958, 1510, 2263
SfcI CTRYAG 2 cut(s) 591, 1877
Sfr274I CTCGAG 1 cut(s) 344
SinI GGWCC 2 cut(s) 1391, 1673
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 2073
SmlI CTYRAG 2 cut(s) 344, 567
SmoI CTYRAG 2 cut(s) 344, 567
SpeI ACTAGT 1 cut(s) 2296
SphI GCATGC 1 cut(s) 833
SseBI AGGCCT 1 cut(s) 1252
SsiI CCGC 4 cut(s) 243, 269, 1028, 1602
SspI AATATT 1 cut(s) 1362
SspMI CTAG 5 cut(s) 390, 2216, 2225, 2297, 2526
StuI AGGCCT 1 cut(s) 1252
StyD4I CCNGG 2 cut(s) 442, 2418
StyI CCWWGG 2 cut(s) 789, 1800
TaiI ACGT 1 cut(s) 900
TaqI TCGA 4 cut(s) 345, 772, 1033, 2263
TatI WGTACW 4 cut(s) 41, 115, 1588, 2373
TauI GCSGC 2 cut(s) 1031, 1604
TfiI GAWTC 7 cut(s) 521, 799, 931, 1008, 1571, 1649, 1847
Tru1I TTAA 3 cut(s) 15, 2484, 2558
Tru9I TTAA 3 cut(s) 15, 2484, 2558
TscAI CASTG 8 cut(s) 546, 625, 715, 918, 1514, 1659, 1795, 2078
TseFI GTSAC 5 cut(s) 1063, 1614, 1654, 1786, 1994
Tsp45I GTSAC 5 cut(s) 1063, 1614, 1654, 1786, 1994
TspDTI ATGAA 7 cut(s) 116, 636, 920, 927, 1224, 1779, 2348
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 8 cut(s) 546, 625, 715, 918, 1514, 1659, 1795, 2078
Van91I CCANNNNNTGG 1 cut(s) 1448
VneI GTGCAC 2 cut(s) 1470, 2292
VpaK11BI GGWCC 2 cut(s) 1391, 1673
XagI CCTNNNNNAGG 2 cut(s) 1173, 1313
XapI RAATTY 3 cut(s) 226, 1892, 2578
XceI RCATGY 2 cut(s) 95, 833
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 607, 1702
XmnI GAANNNNTTC 2 cut(s) 353, 697
XspI CTAG 5 cut(s) 390, 2216, 2225, 2297, 2526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.