pycom15g29620

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007148
Physical Location & Seq
Forward (+)
26538665 .. 26546153
7489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29620.3

Sequence Viewer

Length: 5820 bp
ATGCTGTTTCCCAAGGTGGTGCCAGGGATCGATGTTGGATCAAAAATATTTTCAACCGAAGGAGTTGTGCCAGTGCTGTGGGAGCAGCTACAGAAGGGGATCAAGACTGGCAGTTTGGTTGATAGCTTGTTGACTGGAGCATTAAAGAACCTATCCAGCAGTACTGAGGGATTCTGGACTGCAACCTTCCAAGCTGGAGGAGTGGATATACTTGTGAAATTGCTGACAACTGGACAGTCAAGCACTCAAGCTAATGTGTGCTTTCTTCTTGCATGTATGATGGTGGAAGATGCATCCGTCTGTTCTAAGGTGCTGGCCTCAGAGGCTACCAAACAACTTCTCAAGCTTTTAGGATCTGGAAATGAAGCTTCTGTTAGAGCAGAAACTGCAGGTGCTCTTAAATCTCTTTCTGGCCAGTGTAAAGAAGCAAGGCGGGAAGTAGCTAACTTCAATGGCATTCCTGTATTGATAAATGCTACAATAGCCCCTTCAAAAGAATTCATGCAGGGTGAGTATGCCCAAGCGTTGCAAGAGAATGCTATGTGTGCTTTAGCAAATATTTCTGGTGGGTTGTCATACGTCATCTCAAGCCTTGGGCAAAGCCTTGGATCTTGCACCTCACCTGCTCAAATTGCTGATACATTAGGGGCTTTAGCTTCAGCTCTGATGATATATGATAGTACAGCAGAATCTAATAGAGCATCAGATCCTGTGGTTATTGAGCAGACATTGGTTTCACAGTTCAAACCGCGTTTACCATTTCTTGTTCAGGAACGAACCATAGAGGCCCTTGCAAGTTTGTATGGGAATTCTGTACTCTCAGTTAAACTCGCCAATTCGGAGGCAAAACGTTTGCTTGTTGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTAATGAGAGCTCTTCTTACACTATGCAATAGTGAGGAAAGTCTGTGGCGTGCGCTTCAAGGCCGTGAAGGGGTTCAACTGTTGATATCACTTCTTGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCGTGCTTTCTAATGAGAATGATGAAAGTAAATGGGCTATTACTGCTGCTGGTGGCATACCTCCACTTGTTCAGATTCTGGAGACAGGATCGGCAAAAGCCAAGGAAGATTCAGCATCAATCCTTAGGAACCTATGCAACCACAGTGAAGATATACGTGCATGTGTTGAAAGCGCTGATGCTGTTCCTGCATTATTGTGGCTTCTAAAGAATGGAAGTTCAAACGGAAAAGAAATTGCAGCAAAGACTTTAAATCATTTGATCCATAAATCTGATACAGCAACCATCAGCCAACTTACTGCATTATTGACCAGTGATCTACCAGAATCTAAAGTGTATGTTTTGGATGCTTTAAAAAGTATGCTGTCCGTGGTCCCCCTCAGCGATATATCACGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATAAAATTATTGAGCTCAACTAAAGAAGAAACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATTTTTGGATCCAGGAAGGACTTGCGTGAAAGTAGCATTGCTGTTAAAACTCTTTGGTCCGCCATTAAGTTGATTAGTGTTGAATCTGTATATATCCTTGCAGAGGCCTCACGCTGCCTTGCGGCAATATTTCTTTCGATTAAAGAGAACCGGGATGTGGCAGTTGTTGCTCGAGATGTATTATCTCCATTAGTTGTGCTTGCTAACTCTTCAGTTCTGGAAGTTGCAGAACTAGCAACATGTGCTCTGGCCAATCTTATTTTGGACAGTGAAGTTTCAGAGAAAGCTGTTGCTAAAGATATTATTTTCCCAGCTACTAGAGTTTTGCGTGAAGGCACGGTATCTGGAAAGACCCATGCAGCAGCAGCAATTGCTCGCTTGCTCCATTCTCGTCAAATTGATTATGCTTTAACTGATTGTGTGAATCGTGCTGGAACTGTTCTTGCATTAGTTTCTTTTCTAGAATCTATTAATCATGATTCTGTTGGCACATCAGAGGCACTTGAGGCACTTGCTATTCTATCCGGGTCAGAAGGAGCTACTGGAGAAATAAAACCTGCATGGGCAGTTTTAGCTGAATTCCCGAAAAGCATAACCCCAATAGTTTTGTCCATTGCTGATGCAACACCCTTGTTGCAGGATAAGGCTATTGAAATATTATCACGGCTTTGCAGAGATCAGCCTGATGTATTGGGGGACACAGTTGCTACTGCTTATGGATGTATTTCATCAATTGCCAAAAGGGTGATCAATTCAACGAAATCGAAGGTTAAAACTGGAGGAACTGCACTCCTTATTTGTGTTGCAAAAGTTAGTCATCATAGAGTTGTGGAAGATCTGAGTGAATCAAACTTGTGCACCCATCTAATTCAAGCTCTTGTTGCAATGCTTAGTTCTTTGGGAAACCCAGGGAATAATGAGAATGATTCTATTGGCATTTATAGGCATAGTAAAGAGGAAACAAAGATTGATGAATCTTATTCAAGCACAGTAGTAATTAGTGGTGTCAATTTAGCTATGTGGCTACTGTCTGTTCTTGCCTGTCACGATGAAAGGTGCAAAATTGAGATTATGGAGGCTGGGGCTGTGGAAGTCCTCACTGACAGGATCTCAAATGATTTCTCACCCTACTCTCAGATTGAGTTTAAAGAAGATAGTAGCATATGGATTTGTACTTTGCTGCTGGCAATCTTGTTTCAAAACAGAGACATCATACGAGCACATGCAACCATGAAATCTATACCTGTACTTGCAAACTGGTTGAGATCAGAGGAGATGCCCACCAGATATTTTGCTGCACAGGCAATGGCCAGCCTTTTGTCAGAAGAGTTTGGTTTAGTGCGTTATCCTGAGCAAGTTGCACTCGAGAAGTTGTTCAGAGTTGAAGACATAAGGGTTGGTGCCACTTCTAGGAAAGCAATACCTGCACTTGTTGATCTTCTCAAACCCATTCCAGATCGTCCTGGAGCACCATTTTTAGCACTCGGGCTTCTGACTCAGCTTGCAAAAGACTGTCCATCAAATAAAATTGTAATGGTAGAATCAGGAGCTTTGGAAGCACTGACCAAGTATCTTTCACTTGGCCCTCAAGATGCAACTGAGGAAGCTGCTACAGATCTGTTAGGTATACTATTTGGCAGTGCTGAAATTAGGAGACATGACTCTTCATTTGGTGCTGTTGGTCAACTTGTGGCAGTTTTACGGTTAGGTGGAAGGGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTATTTTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAAGCTGTTCAACCCTTGGTGGAGATTCTCAATACTGGTTCTGAGAAGGAGCAGCATGCTGCAATTGCTGCGTTGGTAAGGCTGCTGAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGACGTTGAAATGAATGCAGTGGATGTTCTTTGCAAGATTCTTTCATCAAATTGCTCAATGGAGCTGAAAGGGGATGCTGCCGAGTTGTGTTGTGTTCTCTTTGGGAATACAAGAATCAGGTCAACAATGGCTGCGGCTCGATGTGTGGAGCCTCTGGTGTCTCTTCTTGTGACTGAGTTCAGTCCTGCGCAGCATTCAGTTGTCCGTGCGTTGGATAAACTTGTTGACGATGAGCAACTGGCAGAACTTGTCGCTGCTCACGGTGCAGTTATTCCTCTTGTAGGTCTTCTCTATGGTAAGAATTACCTGCTCCATGAAGCTATTTCCAGAGCCCTTGTGAAATTAGGGAAAGACAGGCCTGCTTGTAAGATGGAAATGGTGAAAGCTGGAGTGATTGAGAGCATACTTGACATCCTCCATGAGGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAACTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGACCATCTGCTTCAAAAGTGGTTGAGCCTCTTTTTGTGCTGCTAACAAGACCAGAGTTTGGGCCTGATGGGCAGCATAGTGCACTACAAGTTCTTGTCAACATTTTGGAGCATCCACAGTGTCGTTCTGACTATAAGTTGACATCCCACCAAGCTATTGAACCTATTATTCCCTTACTTGATTCTCCTGCACCAGCAGTACAACAGTTGGCCGCAGAGCTTCTGTCACATTTACTCTTTGAAGAGCAACTTCAAAAGGATTCAGTGACTCAGCAAGTGATTGGTCCTCTTATACGGGTTCTTGGCTCTGGTATACACATATTGCAGCAGAGAGCTGTGAAGGCCCTTGTTAGTATTGCGCTAATATGGCCGAATGAAATAGCAAAAGAGGGTGGTGTCACTGAACTGTCCAAGGTGATATTGCAATCAGATCCATCTCTTCCTCATGCCTTGTGGGAATCGGCTGCTGCTGTTTTATCAAGCATTCTGCAGTTTAGTTCTGAATTTTATTTGGAAGTGCCTGTTGCTGTTTTGGTAAGGTTGCTTCGTTCTGGCACAGAAGGCACAGTAATTGGTGCATTGAATGCTCTTCTGGTACTGGAAAGTGACGACGCAACAAGCGCAGAAGCAATGGCTGAAAGTGGTGCTTTAGAGGCTCTTTTAGAACTTCTCAGATCTCATCAGTGTGAGGAAACTGCTGCAAGGCTTTTGGAAGTATTGTTGAACAATGTGAAGATCAGAGAAACTAAGGCTACCAAGTCTGCCATATTACCGTTGTCTCAGTACCTCTTGGATCCACAAACCCAAGCCCAGCAAGCAAGGTTGCTAGCAACTCTAGCTCTTGGTGATCTATTCCAGAACGAGGGCCTTGCTCGAAGTGCTGATGCTGTTTCAGCTTGTCGTGCACTAGTAAATGTGCTTGAAGACCAACCTACTGAAGAAATGAAGGTTGTAGCGATATGTGCTTTGCAAAACCTTGTCATGTACAGTAGATCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGCGTCCAGGTTGTGTTGGATCTGATTGGTTCAAGTGATCCAGATACATCTATTCAGGCTGCAATGTTTGTTAAACTTCTGTTTTCAAACCATACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCCATTACTGCTGCTATCGAGAAGGATTTATGGGCAACTGGAACTGTGAATGAGGAGTATCTGAAAGCTCTGAATGCTCTGTTTAGCAATTTCCCACGACTTAGAGCCACAGAACCTGCAACGTTAAGCATTCCTCATCTTGTTACATCCCTCAAGACAGGGTCTGAGGCAACTCAAGAAGCTGCCTTGGATGCACTCTTTCTTCTTAGGCAAGCTTGGTCAGCATGCCCAGCTGAAGTTTCTAGGGCTCAGTCAATTGCTGCAGCCGATGCAATCCCCTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGGTTTCAGGAGAAGACAGAATTTTTGTTACAATGTTTGCCTGGGACATTGGTGGTGATAATCAAGCGTGGAAATAATATGAAGCAGTCAGTGGGAAACCCTAGTGTTTATTGCAAGATTACACTGGGCAACACTCCACCTAAGCAAACTAAGGTGGTTTCAACTGGCCCTAATCCAGAATGGGATGAAAGCTTTTCATGGTCCTTTGAGAGTCCCCCGAAGGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAAATGGGAAAGAGTTCATTCGGAAAGGTAACCATCCAAATTGATCGCGTAGTAATGCTGGGAGCAGTTGCCGGAGAGTACACTCTGTTGCCAGAAAGCAAAAGTGGGCCCTCACGGAATTTAGAAATAGAGTTCCAGTGGTCTAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

1940

Amino Acids

207.89

Weight (kDa)

5.36

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 380, 631
Acc16I TGCGCA 1 cut(s) 3639
Acc36I ACCTGC 6 cut(s) 380, 631, 2101, 2968, 3765, 5221
AccB1I GGYRCC 3 cut(s) 19, 2936, 3874
AccB7I CCANNNNNTGG 1 cut(s) 4004
AccI GTMKAC 2 cut(s) 3163, 4258
AccII CGCG 2 cut(s) 751, 5715
AciI CCGC 6 cut(s) 433, 749, 1596, 1658, 3584, 4158
AclI AACGTT 2 cut(s) 850, 5219
AcoI YGGCCR 5 cut(s) 412, 1785, 2844, 4155, 4313
AcsI RAATTY 7 cut(s) 497, 808, 1536, 2114, 4448, 5429, 5785
AcuI CTGAAG 4 cut(s) 642, 1731, 4902, 5352
AcvI CACGTG 1 cut(s) 1436
AcyI GRCGYC 1 cut(s) 4971
AfeI AGCGCT 1 cut(s) 1216
AflIII ACRYGT 1 cut(s) 1775
AhlI ACTAGT 1 cut(s) 4852
AjnI CCWGG 6 cut(s) 22, 1547, 2443, 2998, 4974, 5449
Alw26I GTCTC 7 cut(s) 1118, 1460, 2736, 3184, 3615, 4728, 5081
Alw44I GTGCAC 3 cut(s) 2392, 4026, 4848
AlwNI CAGNNNCTG 6 cut(s) 386, 710, 1120, 4958, 5213, 5261
Ama87I CYCGRG 3 cut(s) 1707, 2900, 3020
Aor51HI AGCGCT 1 cut(s) 1216
ApaI GGGCCC 1 cut(s) 5778
ApaLI GTGCAC 3 cut(s) 2392, 4026, 4848
ApoI RAATTY 7 cut(s) 497, 808, 1536, 2114, 4448, 5429, 5785
ArsI GACNNNNNNTTYG 6 cut(s) 97, 129, 3188, 3220, 5416, 5448
AseI ATTAAT 1 cut(s) 2007
Asp700I GAANNNNTTC 4 cut(s) 972, 2909, 3253, 5680
AspLEI GCGC 5 cut(s) 955, 1217, 3640, 4306, 4568
AsuC2I CCSGG 2 cut(s) 1688, 2062
AsuHPI GGTGA 8 cut(s) 521, 612, 2293, 2652, 3841, 4372, 4802, 5476
AsuNHI GCTAGC 1 cut(s) 4771
AvaI CYCGRG 3 cut(s) 1707, 2900, 3020
AvaII GGWCC 5 cut(s) 1414, 1593, 3947, 4229, 5610
AxyI CCTNAGG 1 cut(s) 1166
BaeGI GKGCMC 4 cut(s) 2396, 4030, 4852, 5778
BalI TGGCCA 3 cut(s) 414, 1787, 2846
BamHI GGATCC 2 cut(s) 1544, 4738
BanI GGYRCC 3 cut(s) 19, 2936, 3874
BanII GRGCYC 5 cut(s) 913, 1490, 3784, 5347, 5778
BarI GAAGNNNNNNTAC 2 cut(s) 4473, 4505
BbrPI CACGTG 1 cut(s) 1436
BbsI GAAGAC 4 cut(s) 2928, 3728, 4875, 5429
BbvCI CCTCAGC 2 cut(s) 1421, 4959
BceAI ACGGC 2 cut(s) 948, 2216
BcgI CGANNNNNNTGC 2 cut(s) 4796, 4830
BciT130I CCWGG 6 cut(s) 24, 1549, 2445, 3000, 4976, 5451
BclI TGATCA 2 cut(s) 867, 2283
BcnI CCSGG 2 cut(s) 1688, 2062
BcoDI GTCTC 7 cut(s) 1118, 1460, 2736, 3184, 3615, 4728, 5081
BcuI ACTAGT 1 cut(s) 4852
BfmI CTRYAG 5 cut(s) 89, 387, 3147, 4433, 5358
BfoI RGCGCY 1 cut(s) 1218
BfuAI ACCTGC 6 cut(s) 380, 631, 2101, 2968, 3765, 5221
BglI GCCNNNNNGGC 2 cut(s) 323, 4015
BglII AGATCT 4 cut(s) 2371, 3151, 4619, 4937
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 8 cut(s) 24, 1549, 1688, 2062, 2445, 3000, 4976, 5451
Bme18I GGWCC 5 cut(s) 1414, 1593, 3947, 4229, 5610
BmeT110I CYCGRG 3 cut(s) 1707, 2900, 3020
BmrFI CCNGG 8 cut(s) 24, 1549, 1688, 2062, 2445, 3000, 4976, 5451
BmrI ACTGGG 1 cut(s) 5543
BmtI GCTAGC 1 cut(s) 4775
BmuI ACTGGG 1 cut(s) 5543
BpiI GAAGAC 4 cut(s) 2928, 3728, 4875, 5429
BpmI CTGGAG 7 cut(s) 156, 216, 1142, 2100, 2334, 3021, 3858
Bpu10I CCTNAGC 6 cut(s) 1421, 2886, 3439, 3942, 4959, 5550
BpuEI CTTGAG 8 cut(s) 231, 326, 571, 1491, 2060, 3108, 5234, 5256
BpuMI CCSGG 2 cut(s) 1688, 2062
Bsa29I ATCGAT 1 cut(s) 30
BsaAI YACGTR 2 cut(s) 1199, 1436
BsaHI GRCGYC 1 cut(s) 4971
Bse21I CCTNAGG 1 cut(s) 1166
Bse3DI GCAATG 7 cut(s) 1572, 2148, 2427, 2847, 3936, 4581, 5037
BseBI CCWGG 6 cut(s) 24, 1549, 2445, 3000, 4976, 5451
BseCI ATCGAT 1 cut(s) 30
BseMI GCAATG 7 cut(s) 1572, 2148, 2427, 2847, 3936, 4581, 5037
BseRI GAGGAG 3 cut(s) 213, 2822, 5165
BseSI GKGCMC 4 cut(s) 2396, 4030, 4852, 5778
BseYI CCCAGC 5 cut(s) 1846, 2615, 4755, 5326, 5725
BsgI GTGCAG 6 cut(s) 1047, 2307, 2817, 2946, 3735, 4119
Bsh1236I CGCG 2 cut(s) 751, 5715
BshNI GGYRCC 3 cut(s) 19, 2936, 3874
BshVI ATCGAT 1 cut(s) 30
BsiHKCI CYCGRG 3 cut(s) 1707, 2900, 3020
BsiSI CCGG 3 cut(s) 1687, 2061, 5739
BslFI GGGAC 5 cut(s) 1400, 2246, 3960, 5467, 5607
BsmAI GTCTC 7 cut(s) 1118, 1460, 2736, 3184, 3615, 4728, 5081
BsmFI GGGAC 5 cut(s) 1400, 2246, 3960, 5467, 5607
BsmI GAATGC 9 cut(s) 456, 541, 3319, 3469, 3643, 4428, 4534, 5176, 5226
BsoBI CYCGRG 3 cut(s) 1707, 2900, 3020
Bsp120I GGGCCC 1 cut(s) 5774
Bsp1407I TGTACA 1 cut(s) 4929
BspACI CCGC 6 cut(s) 433, 749, 1596, 1658, 3584, 4158
BspDI ATCGAT 1 cut(s) 30
BspFNI CGCG 2 cut(s) 751, 5715
BspHI TCATGA 1 cut(s) 2011
BspMAI CTGCAG 3 cut(s) 391, 4437, 5362
BspMI ACCTGC 6 cut(s) 380, 631, 2101, 2968, 3765, 5221
BspOI GCTAGC 1 cut(s) 4775
BspQI GCTCTTC 3 cut(s) 918, 4182, 4539
BspT107I GGYRCC 3 cut(s) 19, 2936, 3874
BsrDI GCAATG 7 cut(s) 1572, 2148, 2427, 2847, 3936, 4581, 5037
BsrGI TGTACA 1 cut(s) 4929
BssNAI GTATAC 2 cut(s) 3164, 4259
BssNI GRCGYC 1 cut(s) 4971
BssT1I CCWWGG 7 cut(s) 12, 592, 604, 1143, 3345, 4356, 5283
Bst1107I GTATAC 2 cut(s) 3164, 4259
Bst2UI CCWGG 6 cut(s) 24, 1549, 2445, 3000, 4976, 5451
Bst6I CTCTTC 8 cut(s) 918, 1750, 2856, 3205, 3618, 4182, 4389, 4539
BstACI GRCGYC 1 cut(s) 4971
BstAUI TGTACA 1 cut(s) 4929
BstBAI YACGTR 2 cut(s) 1199, 1436
BstDSI CCRYGG 1 cut(s) 1410
BstEII GGTNACC 1 cut(s) 5695
BstENI CCTNNNNNAGG 3 cut(s) 1637, 3729, 3869
BstFNI CGCG 2 cut(s) 751, 5715
BstH2I RGCGCY 1 cut(s) 1218
BstHHI GCGC 5 cut(s) 955, 1217, 3640, 4306, 4568
BstMAI GTCTC 7 cut(s) 1118, 1460, 2736, 3184, 3615, 4728, 5081
BstNI CCWGG 6 cut(s) 24, 1549, 2445, 3000, 4976, 5451
BstNSI RCATGY 6 cut(s) 276, 1206, 1779, 2762, 3389, 5325
BstPI GGTNACC 1 cut(s) 5695
BstSCI CCNGG 8 cut(s) 22, 1547, 1686, 2060, 2443, 2998, 4974, 5449
BstSFI CTRYAG 5 cut(s) 89, 387, 3147, 4433, 5358
BstSLI GKGCMC 4 cut(s) 2396, 4030, 4852, 5778
BstUI CGCG 2 cut(s) 751, 5715
BstV2I GAAGAC 4 cut(s) 2928, 3728, 4875, 5429
BstXI CCANNNNNNTGG 1 cut(s) 78
BstZ17I GTATAC 2 cut(s) 3164, 4259
Bsu15I ATCGAT 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 1166
BsuTUI ATCGAT 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 1410
BtsI GCAGTG 3 cut(s) 1034, 3181, 3474
BveI ACCTGC 6 cut(s) 380, 631, 2101, 2968, 3765, 5221
CaiI CAGNNNCTG 6 cut(s) 386, 710, 1120, 4958, 5213, 5261
CciI TCATGA 1 cut(s) 2011
CfoI GCGC 5 cut(s) 955, 1217, 3640, 4306, 4568
ClaI ATCGAT 1 cut(s) 30
CseI GACGC 2 cut(s) 4565, 4960
CspCI CAANNNNNGTGG 2 cut(s) 5633, 5668
DraI TTTAAA 3 cut(s) 1293, 1395, 2683
EaeI YGGCCR 5 cut(s) 412, 1785, 2844, 4155, 4313
Eam1104I CTCTTC 8 cut(s) 918, 1750, 2856, 3205, 3618, 4182, 4389, 4539
EarI CTCTTC 8 cut(s) 918, 1750, 2856, 3205, 3618, 4182, 4389, 4539
EciI GGCGGA 1 cut(s) 1585
Ecl136II GAGCTC 2 cut(s) 911, 1488
Eco130I CCWWGG 7 cut(s) 12, 592, 604, 1143, 3345, 4356, 5283
Eco147I AGGCCT 2 cut(s) 1643, 3808
Eco24I GRGCYC 5 cut(s) 913, 1490, 3784, 5347, 5778
Eco32I GATATC 1 cut(s) 987
Eco47I GGWCC 5 cut(s) 1414, 1593, 3947, 4229, 5610
Eco47III AGCGCT 1 cut(s) 1216
Eco53kI GAGCTC 2 cut(s) 911, 1488
Eco57I CTGAAG 4 cut(s) 642, 1731, 4902, 5352
Eco72I CACGTG 1 cut(s) 1436
Eco81I CCTNAGG 1 cut(s) 1166
Eco88I CYCGRG 3 cut(s) 1707, 2900, 3020
Eco91I GGTNACC 1 cut(s) 5695
EcoICRI GAGCTC 2 cut(s) 911, 1488
EcoNI CCTNNNNNAGG 3 cut(s) 1637, 3729, 3869
EcoO109I RGGNCCY 4 cut(s) 787, 4288, 4810, 5775
EcoO65I GGTNACC 1 cut(s) 5695
EcoRI GAATTC 3 cut(s) 497, 808, 2114
EcoRII CCWGG 6 cut(s) 22, 1547, 2443, 2998, 4974, 5449
EcoRV GATATC 1 cut(s) 987
EcoT14I CCWWGG 7 cut(s) 12, 592, 604, 1143, 3345, 4356, 5283
EcoT22I ATGCAT 1 cut(s) 295
EcoT38I GRGCYC 5 cut(s) 913, 1490, 3784, 5347, 5778
ErhI CCWWGG 7 cut(s) 12, 592, 604, 1143, 3345, 4356, 5283
FalI AAGNNNNNCTT 4 cut(s) 4179, 4211, 4576, 4608
FaqI GGGAC 5 cut(s) 1400, 2246, 3960, 5467, 5607
FauI CCCGC 1 cut(s) 426
FauNDI CATATG 1 cut(s) 2699
FbaI TGATCA 2 cut(s) 867, 2283
FblI GTMKAC 2 cut(s) 3163, 4258
FriOI GRGCYC 5 cut(s) 913, 1490, 3784, 5347, 5778
FspI TGCGCA 1 cut(s) 3639
GlaI GCGC 5 cut(s) 954, 1216, 3639, 4305, 4567
GsaI CCCAGC 5 cut(s) 1850, 2619, 4759, 5330, 5729
GsuI CTGGAG 7 cut(s) 156, 216, 1142, 2100, 2334, 3021, 3858
HaeII RGCGCY 1 cut(s) 1218
HapII CCGG 3 cut(s) 1687, 2061, 5739
HgaI GACGC 2 cut(s) 4565, 4960
HhaI GCGC 5 cut(s) 955, 1217, 3640, 4306, 4568
Hin1I GRCGYC 1 cut(s) 4971
Hin6I GCGC 5 cut(s) 953, 1215, 3638, 4304, 4566
HinP1I GCGC 5 cut(s) 953, 1215, 3638, 4304, 4566
HincII GTYRAC 6 cut(s) 132, 3221, 3573, 3676, 4045, 4086
HindII GTYRAC 6 cut(s) 132, 3221, 3573, 3676, 4045, 4086
HindIII AAGCTT 5 cut(s) 344, 366, 3286, 5310, 5599
HpaII CCGG 3 cut(s) 1687, 2061, 5739
HphI GGTGA 8 cut(s) 521, 612, 2293, 2652, 3841, 4372, 4802, 5476
Hpy99I CGWCG 1 cut(s) 4559
HpyCH4IV ACGT 6 cut(s) 579, 850, 1198, 1435, 3453, 5219
HpySE526I ACGT 6 cut(s) 579, 850, 1198, 1435, 3453, 5219
Hsp92I GRCGYC 1 cut(s) 4971
HspAI GCGC 5 cut(s) 953, 1215, 3638, 4304, 4566
Ksp22I TGATCA 2 cut(s) 867, 2283
LguI GCTCTTC 3 cut(s) 918, 4182, 4539
MaeII ACGT 6 cut(s) 579, 850, 1198, 1435, 3453, 5219
MaeIII GTNAC 9 cut(s) 2579, 3619, 4170, 4210, 4342, 4550, 5239, 5436, 5695
MfeI CAATTG 5 cut(s) 1461, 1905, 2268, 3393, 5352
MlsI TGGCCA 3 cut(s) 414, 1787, 2846
MluNI TGGCCA 3 cut(s) 414, 1787, 2846
MlyI GAGTC 4 cut(s) 3025, 3191, 4207, 5629
MmeI TCCRAC 3 cut(s) 16, 3642, 4965
Mox20I TGGCCA 3 cut(s) 414, 1787, 2846
Mph1103I ATGCAT 1 cut(s) 295
MroXI GAANNNNTTC 4 cut(s) 972, 2909, 3253, 5680
MscI TGGCCA 3 cut(s) 414, 1787, 2846
MslI CAYNNNNRTG 2 cut(s) 1237, 4629
Msp20I TGGCCA 3 cut(s) 414, 1787, 2846
MspA1I CMGCKG 1 cut(s) 5330
MspI CCGG 3 cut(s) 1687, 2061, 5739
MspR9I CCNGG 8 cut(s) 24, 1549, 1688, 2062, 2445, 3000, 4976, 5451
MunI CAATTG 5 cut(s) 1461, 1905, 2268, 3393, 5352
Mva1269I GAATGC 9 cut(s) 456, 541, 3319, 3469, 3643, 4428, 4534, 5176, 5226
MvaI CCWGG 6 cut(s) 24, 1549, 2445, 3000, 4976, 5451
MvnI CGCG 2 cut(s) 751, 5715
NciI CCSGG 2 cut(s) 1688, 2062
NdeI CATATG 1 cut(s) 2699
NheI GCTAGC 1 cut(s) 4771
NmeAIII GCCGAG 1 cut(s) 3556
NmuCI GTSAC 6 cut(s) 2579, 3619, 4170, 4210, 4342, 4550
NsbI TGCGCA 1 cut(s) 3639
NsiI ATGCAT 1 cut(s) 295
NspI RCATGY 6 cut(s) 276, 1206, 1779, 2762, 3389, 5325
PaeI GCATGC 2 cut(s) 3389, 5325
PaeR7I CTCGAG 2 cut(s) 1707, 2900
PagI TCATGA 1 cut(s) 2011
PaqCI CACCTGC 2 cut(s) 380, 631
PasI CCCWGGG 1 cut(s) 2444
PceI AGGCCT 2 cut(s) 1643, 3808
PciI ACATGT 1 cut(s) 1775
PciSI GCTCTTC 3 cut(s) 918, 4182, 4539
PctI GAATGC 9 cut(s) 456, 541, 3319, 3469, 3643, 4428, 4534, 5176, 5226
PdmI GAANNNNTTC 4 cut(s) 972, 2909, 3253, 5680
PflFI GACNNNGTC 1 cut(s) 5257
PflMI CCANNNNNTGG 1 cut(s) 4004
PfoI TCCNGGA 2 cut(s) 1547, 2998
PleI GAGTC 4 cut(s) 3025, 3191, 4207, 5628
PmaCI CACGTG 1 cut(s) 1436
PmlI CACGTG 1 cut(s) 1436
PpsI GAGTC 4 cut(s) 3025, 3191, 4207, 5628
Ppu21I YACGTR 2 cut(s) 1199, 1436
PscI ACATGT 1 cut(s) 1775
PshBI ATTAAT 1 cut(s) 2007
Psp124BI GAGCTC 2 cut(s) 913, 1490
Psp1406I AACGTT 2 cut(s) 850, 5219
Psp6I CCWGG 6 cut(s) 22, 1547, 2443, 2998, 4974, 5449
PspCI CACGTG 1 cut(s) 1436
PspEI GGTNACC 1 cut(s) 5695
PspFI CCCAGC 5 cut(s) 1846, 2615, 4755, 5326, 5725
PspGI CCWGG 6 cut(s) 22, 1547, 2443, 2998, 4974, 5449
PspOMI GGGCCC 1 cut(s) 5774
PstI CTGCAG 3 cut(s) 391, 4437, 5362
PstNI CAGNNNCTG 6 cut(s) 386, 710, 1120, 4958, 5213, 5261
PsyI GACNNNGTC 1 cut(s) 5257
PvuII CAGCTG 1 cut(s) 5330
RseI CAYNNNNRTG 2 cut(s) 1237, 4629
SacI GAGCTC 2 cut(s) 913, 1490
SapI GCTCTTC 3 cut(s) 918, 4182, 4539
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 4 cut(s) 3025, 3191, 4207, 5629
ScrFI CCNGG 8 cut(s) 24, 1549, 1688, 2062, 2445, 3000, 4976, 5451
SfcI CTRYAG 5 cut(s) 89, 387, 3147, 4433, 5358
Sfr274I CTCGAG 2 cut(s) 1707, 2900
SinI GGWCC 5 cut(s) 1414, 1593, 3947, 4229, 5610
SlaI CTCGAG 2 cut(s) 1707, 2900
SmiMI CAYNNNNRTG 2 cut(s) 1237, 4629
SpeI ACTAGT 1 cut(s) 4852
SphI GCATGC 2 cut(s) 3389, 5325
SseBI AGGCCT 2 cut(s) 1643, 3808
SsiI CCGC 6 cut(s) 433, 749, 1596, 1658, 3584, 4158
SspI AATATT 5 cut(s) 48, 559, 1665, 2193, 3918
SstI GAGCTC 2 cut(s) 913, 1490
StuI AGGCCT 2 cut(s) 1643, 3808
StyD4I CCNGG 8 cut(s) 22, 1547, 1686, 2060, 2443, 2998, 4974, 5449
StyI CCWWGG 7 cut(s) 12, 592, 604, 1143, 3345, 4356, 5283
TaiI ACGT 6 cut(s) 582, 853, 1201, 1438, 3456, 5222
TaqI TCGA 9 cut(s) 30, 1673, 1708, 2300, 2901, 3328, 3589, 4819, 5115
TatI WGTACW 8 cut(s) 161, 680, 814, 2708, 2782, 4144, 4929, 5745
TauI GCSGC 3 cut(s) 1661, 3587, 4160
TseFI GTSAC 6 cut(s) 2579, 3619, 4170, 4210, 4342, 4550
Tsp45I GTSAC 6 cut(s) 2579, 3619, 4170, 4210, 4342, 4550
TspGWI ACGGA 5 cut(s) 286, 1281, 1399, 3644, 5797
Tth111I GACNNNGTC 1 cut(s) 5257
Van91I CCANNNNNTGG 1 cut(s) 4004
VneI GTGCAC 3 cut(s) 2392, 4026, 4848
VpaK11BI GGWCC 5 cut(s) 1414, 1593, 3947, 4229, 5610
VspI ATTAAT 1 cut(s) 2007
XagI CCTNNNNNAGG 3 cut(s) 1637, 3729, 3869
XapI RAATTY 7 cut(s) 497, 808, 1536, 2114, 4448, 5429, 5785
XbaI TCTAGA 1 cut(s) 1996
XceI RCATGY 6 cut(s) 276, 1206, 1779, 2762, 3389, 5325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XhoI CTCGAG 2 cut(s) 1707, 2900
XmiI GTMKAC 2 cut(s) 3163, 4258
XmnI GAANNNNTTC 4 cut(s) 972, 2909, 3253, 5680
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.