MD15G1329300.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007148
Physical Location & Seq
Reverse (-)
36263039 .. 36273260
10222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1329300.v1.1.491

Sequence Viewer

Length: 6444 bp
ATGGCAACCACAGTGGGATGGAGGTATGCTGCCACCAATGGCAGCACCCTTACACCTAATGATCTGGAAAGGAATGGTGATGCAAAAGTTCAAGATTCAGAGCCCCCAACACCACATTCTCTTATAAAAATGGGTTCACGAGACCGTAGTAGTAGCATGGAGGATGCAGATGGGACATTGGCAAGTGTTGCCCAATGCATTGAGCAGCTGCGTCAAAGTTCCTCATCTGTACAAGAGAAGGAGTATTCGTTGAAGCAGTTGCTTGAGCTTATTGATACTCGTGAAAATGCTTTCAGTGCTGTTGGATCTCACTCTCAAGCAGTCCCAGTGCTTGTTTCTCTTCTTCGATCAGGTTCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTCTCTCTGCAAGGAGAATGAACTAAGGGTGAAAGTCTTGCTTGGAGGATGCATTCCGCCTTTGCTTGGTCTACTCAGGTCCAGCTCAGCAGAAGGGCAAATTGCAGCTGCAAAGACAATTTATGCTGTTTCCCAAGGTGGTGCCAGGGATCATGTTGGATCAAAAATATTTTCAACCGAAGGAGTTGTGCCAGTGCTCTGGGAGCAGCTACAAAAAGGGATCAAGACTGGCAGTTTGGTTGATAGCTTGCTGACTGGAGCATTAAAGAACCTATCCAGCAGTACTGAGGGATTCTGGACTGCAACCTTCCAAGCTGGAGGAGTAGATATACTTGTGAAGTTGCTGACAACTGGACAGTCAAGCACTCAAGCTAACGTGTGCTTTCTTCTTGCATGTATGATGGTGGAAGATGCATCCGTCTGTTCTAAGGTGCTGGCCTCAGAGGCTACCAAACAACTTCTCAAGCTTTTAGGATCTGGAAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTCTTAAATCTCTTTCTGGCCAGTGTAAAGAAGCAAGGCGGGAAGTAGCTAACTTCAACGGCATTCCTGTATTGATAAATGCTACAATAGCCCCTTCAAAAGAATTCATGCAGGGTGAGTATGCCCAAGCGTTGCAAGAGAATGCTATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTCATCTCAAGCCTTGGGCAAAGCCTTGGATCTTGCACCTCACCTGCTCAAATTGCTGATACATTAGGGGCTTTAGCTTCAGCTCTGATGATATATGATAGTACAGCAGAATCTAATAGAGCATCAGATCCTGTGGTTATTGAGCAGACATTGGTTTCACAGTTCAAACCGCGTTTACCATTTCTTGTTCAGGAACGAACCATAGAGGCCCTTGCAAGTTTGTATGGGAATTCTGTACTCTCAGTTAAACTCGCCAATTCAGAGGCAAAGCGTTTGCTTGTTGGTTTGATCACAATGGCAACAAATGAAGTCCAGGATGAGCTAATGAGAGCTCTTCTTACACTTTGCAATAGTGAGGAAAGTCTGTGGCGTGCGCTTCAAGGCCGTGAAGGGGTTCAACTGTTGATATCACTTCTTGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTGCTTTCTAATGAGAATGATGAAAGTAAATGGGCTATTACTGCTGCTGGTGGCATACCTCCACTTGTTCAGATTCTGGAGACGGGATCGGCAAAAGCCAAGGAAGATTCAGCATCAATCCTTAGGAATCTATGCAACCACAGTGAAGATATACGTGCATGTGTCGAAAGCGCTGATGCTGTTCCTGCATTATTGTGGCTTCTAAAGAATGGAAGTTCAAACGGAAAAGAAATTGCAGCAAAGACTTTAAATCATTTGATCCATAAATCTGATACAGCAACCATCAGCCAACTTACTGCATTGTTGACCAGTGATCTACCTGAATCTAAAGTGTATGTTTTGGATGCTTTAAAAAGTATGCTGTCTGTAGTCCCCCTCAACGATATATCACGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATAAAATTATTGAGCTCAACTAAAGAAGAAACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATTTTTGGATCCAGGAAGGACTTGCGTGAAAGTAGCATTGCTGTTAAAACTCTTTGGTCCGCCATTAAGTTGATTAGTGTTGAATCTGTATATATCCTTGCAGAGGCCTCACGCTGCCTTGCGGCAATATTTCTTTCGATTAAAGAAAACCGGGATGTGGCAGCTGTTGCTCGAGATGTATTATCTCCATTAGTTGTGCTTGCTAACTCTTCAGTTCTGGAAGTTGTAGAACTAGCAACATGTGCTCTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAGAAAGCAGTTGCTGAAGATATTATTTTCCCAGCTACTAGAGTTTTGCGTGAAGGCACGGTATCTGGAAAGACCCATGCAGCAGCAGCAATTGCTCGCTTGCTCCATTCTCGTCAAATTGATTATGCTTTAACTGATTGTGTGAATCGTGCTGGAACTGTTCTTGCATTAGTTTCTTTTCTAGAATCTATTAATCATGATTCTGTTGCCACATCAGAGGCACTTGAGGCACTTGCTATTCTATCTGGGTCAGAAGGAGCTACTGGAGAAATAAAACCTGCATGGGCAGTTTTAGCTGAATTCCCGAAAAGCATAACCCCAATAGTTTTGTCCATTGCTGATGCAACACCCTTGTTGCAGGATAAGGCTATTGAAATATTATCACGGCTTTGCAGAGATCAGCCTGATGTATTGGGGGACACAGTTGCTACTGCTTATGGATGTATTTCATCAATTGCCAAAAGGGTGATCAATTCAACGAAATCAAAGGTTAAAACTGGAGGAACTGCACTCCTTATTTGTGTTGCAAAAGTTAGTTATCAGAGAGTTGTCGAAGATCTGAGTGAATCAAACTTATGCACCCATCTAATTCAAGCTCTTGTTGCAATGCTTAGTTTTTTGGGAAACCCAGGGAATAATGAGAATGATTCTATTGGCATTTATAGGCATACTAAAGAGGAAACAAGGATTGATGAATCTTATTCAAGCACAGGAGTAATTAGTGGTGTCAATTTAGCTATGTGGCTACTGTCTGTTCTTGCCTGTCACGATGAAAGGTGCAAAATTGAGATTATGGAGGCTGGGGCTGTGGAAGTCCTCACTGACAGGATCTCAAATGATTTCTCACCCTACTCTCAGATTGAGTTTAAAGAAGATAGTAGCATATGGATTTGTACTTTGCTGCTGGCAATCTTGTTTCAAAACAGAGACATCATACGAGCACATGCAACCATGAAATCTATACCAGTACTTGCAAACTGGTTGAGATCAGAGGAGATGCCCACCAGATATTTTGCTGCACAGGCGATGGCCAGCCTTGTCTGTAATGGTAGCAGGGGGACTCTCCTATCTGTTGCAAATTCTGGAGCAGCAGGCGGGCTCATTTCCCTACTTGGCTGTGCGGATGTTGATATAAGTGATCTTTTGCAGTTGTCAGAAGAGTTTGGTTTAGTGCGTTATCCTGAGCAAGTTGCACTCGAGAGGTTGTTCAGAGTTGAAGACATAAGGGTTGGTGCCACTTCTAGGAAAGCAATACCTGCACTTGTTGATCTTCTCAAACCCATTCCAGATCGTCCTGGAGCTCCATTTTTAGCACTCGGGCTTCTGACTCAGCTTGCAAAAGACTGTCCATCAAATAAAATTGTAATGGTAGAATCAGGAGCTTTGGAAGCACTGACCAAGTATCTTTCACTTGGCCCTCAAGATGCAACTGAGGAAGCTGCTACAGATCTGTTAGGTATACTATTTGGCAGTGCTGAAATTAGGAGACATGACTCTTCATTTGGTGCTGTTGGTCAACTTGTGGCAGTTTTACGGTTAGGTGGAAGGGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTATTTTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAAGCTGTTCAACCCTTGGTGGAGATTCTCAATACTGGTTCTGAGAAGGAGCAGCATGCTGCAATTGCTGCGTTGGTAAGGCTGCTGAGTGAAAATCCATCAAGAGCCTTAGCAGTAGCAGACGTTGAAATGAATGCAGTGGATGTTCTTTGCAAGATTCTTTCATCAAATTGCTCAATGGAGCTGAAAGGGGATGCTGCCGAGTTGTGTTGTGTTCTCTTTGGGAATACAAGAATCAGGTCAACAATGGCTGCGGCTCGATGTGTGGAGCCTCTGGTGTCTCTTCTTGTGACTGAGTTCAGTCCTGCGCAGCATTCAGTTGTCCGTGCGTTGGATAAACTTGTTGATGATGAACAACTGGCAGAACTTGTCGCAGCTCACGGTGCAGTTATTCCTCTTGTAGGTCTTCTCTATGGTAAGAATTACCTGCTCCATGAAGCTATTTCCAGAGCCCTTGTGAAATTAGGGAAAGACAGGCCTGCTTGTAAGATGGAAATGGTGAAAGCTGGAGTGATTGAGAGCATACTTGACATCCTCCATGAGGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAACTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGACCATCTGCTTCAAAAGTGGTTGAGCCTCTGTTTGTGCTGCTAACAAGACCAGAGTTTGGACCTGATGGGCAGCATAGTGCACTACAAGTTCTTGTCAACATTTTGGAGCATCCACAGTGTCGTTCTGACTATAAGTTGACATCCCACCAAGCTATTGAACCTATTATTCCCTTACTTGATTCTCCTGCGCCAGCAGTACAACAGTTGGCAGCTGAGCTTCTGTCACATTTACTCTTTGAAGAGCAACTTCAAAAGGATTCAGTGACTCAGCAAGTGATTGGTCCTCTTATACGGGTTCTTGGCTCTGGTATACACATATTACAGCAGAGAGCTGTAAAGGCCCTTGTTAGTATTGCGCTAATATGGCCAAATGAAATAGCAAAAGAGGGTGGTGTCACTGAACTGTCCAAGGTGATATTGCAATCAGATCCATCTCTTCCTCATGCCTTGTGGGAATCGGCTGCCGCTGTTTTATCAAGCATTCTGCAGTTTAGTTCTGAATTTTATTTGGAAGTGCCTGTTGCTGTTTTGGTAAGGTTGCTTCGTTCTGGCTCAGAAGGCACAGTAATTGGTGCATTGAACGCTCTTCTGGTACTGGAAAGTGACGACGCTACAAGCGCAGAAGCAATGGCTGAAAGTGGTGCTTTAGAGGCTCTTTTAGAACTTCTCAGATCTCATCAGTGTGAGGAAACTGCTGCAAGGCTTTTGGAAGTATTGTTGAACAATGTGAAGATCAGAGAAACTAAGGCTACCAAGTCTGCCATATTACCATTGTCTCAGTACCTCTTGGATCCACAAACCCAAGCCCAGCAAGCAAGGTTGCTAGCAACTCTAGCTCTTGGTGATCTATTCCAGAACGAGGGCCTTGCTCGAAGTACTGACGCTGTTTCAGCTTGTCGTGCACTAGTAAATGTGCTTGAAGACCAACCTACTGAAGAAATGAAGGTTGTAGCGATATGTGCTTTGCAAAACCTTGTCATGTACAGTAGATCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGCGTCCAGGTTGTGTTGGATCTAATTGGTTCAAGTGATCCAGATACATCTATTCAGGCCGCAATGTTTGTTAAACTTCTCTTTTCAAACCATACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCCATTACTGCTGCTATTGAGAAGGATTTATGGGCAACTGGAACTGTGAATGAGGAGTATCTGAAAGCTCTGAATGCTCTGTTTAGCAATTTCCCACGACTTAGAGCCACAGAACCTGCAACGTTAAGCATTCCTCATCTTGTTACATCCCTCAAGACAGGGTCTGAGGCAACTCAAGAAGCTGCCTTGGATGCACTCTTTCTTCTTAGGCAAGCTTGGTCAGCATGCCCAGCTGAAGTTTCTAGGGCTCAGTCAATTGCTGCAGCTGATGCAATCCCCTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGGTTTCAGGAGAAGACAGAATTTTTGTTACAATGTTTGCCTGGGACATTGGTGGTGATAATCAAGCGTGGAAATAATATGAAGCAGTCAGTGGGAAACCCTAGTGTTTATTGCAAGATTACACTGGGCAACACTCCACCTAAGCAAACCAAGGTGGTTTCAACTGGCCCTAATCCAGAATGGGATGAAAGCTTTTCGTGGTCCTTTGAGAGTCCCCCGAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAAATGGGAAAGAGTTCATTCGGAAAGGTAACCATCCAAATTGATCGCGTAGTAATGCTGGGAGCAGTTGCCGGAGAGTACACTCTGTTACCAGAAAGCAAAAGCGGGCCCTCACGGAATTTAGAAATAGAGTTCCAGTGGTCTAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2148

Amino Acids

229.58

Weight (kDa)

5.32

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 62 - 313 8.1e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 427 - 604 6.6e-10 ARMC5
C2 PF00168 2026 - 2120 6.2e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 125
AarI CACCTGC 2 cut(s) 893, 1144
Acc16I TGCGCA 1 cut(s) 4263
Acc36I ACCTGC 6 cut(s) 893, 1144, 2614, 3592, 4389, 5845
AccB1I GGYRCC 3 cut(s) 533, 3560, 4498
AccB7I CCANNNNNTGG 1 cut(s) 4628
AccI GTMKAC 3 cut(s) 463, 3787, 4882
AccII CGCG 2 cut(s) 1264, 6339
AclI AACGTT 1 cut(s) 5843
AcoI YGGCCR 3 cut(s) 925, 3357, 4937
AcsI RAATTY 8 cut(s) 1010, 1321, 2049, 2627, 3406, 5072, 6053, 6409
AcuI CTGAAG 5 cut(s) 1155, 2244, 2364, 5526, 5976
AcvI CACGTG 1 cut(s) 1949
AcyI GRCGYC 1 cut(s) 5595
AfeI AGCGCT 1 cut(s) 1729
AflIII ACRYGT 2 cut(s) 768, 2288
AhlI ACTAGT 1 cut(s) 5476
AjnI CCWGG 7 cut(s) 536, 1404, 2060, 2956, 3622, 5598, 6073
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
Alw26I GTCTC 8 cut(s) 135, 1631, 1973, 3249, 3808, 4239, 5352, 5705
Alw44I GTGCAC 2 cut(s) 4650, 5472
AlwNI CAGNNNCTG 7 cut(s) 899, 1223, 1633, 2342, 5582, 5837, 5885
Ama87I CYCGRG 3 cut(s) 2220, 3524, 3644
Aor51HI AGCGCT 1 cut(s) 1729
ApaI GGGCCC 1 cut(s) 6402
ApaLI GTGCAC 2 cut(s) 4650, 5472
ApoI RAATTY 8 cut(s) 1010, 1321, 2049, 2627, 3406, 5072, 6053, 6409
ArsI GACNNNNNNTTYG 6 cut(s) 610, 642, 3812, 3844, 6040, 6072
AseI ATTAAT 1 cut(s) 2520
Asp700I GAANNNNTTC 3 cut(s) 1485, 3877, 6304
AspLEI GCGC 6 cut(s) 1468, 1730, 4264, 4762, 4930, 5192
AsuC2I CCSGG 1 cut(s) 2201
AsuNHI GCTAGC 1 cut(s) 5395
AvaI CYCGRG 3 cut(s) 2220, 3524, 3644
AvaII GGWCC 6 cut(s) 471, 2106, 4571, 4631, 4853, 6234
AxyI CCTNAGG 1 cut(s) 1679
BaeGI GKGCMC 3 cut(s) 4654, 5476, 6402
BalI TGGCCA 3 cut(s) 927, 3359, 4939
BamHI GGATCC 2 cut(s) 2057, 5362
BanI GGYRCC 3 cut(s) 533, 3560, 4498
BanII GRGCYC 8 cut(s) 105, 1426, 2003, 3429, 3631, 4408, 5971, 6402
BarI GAAGNNNNNNTAC 2 cut(s) 5097, 5129
BauI CACGAG 2 cut(s) 138, 279
BbrPI CACGTG 1 cut(s) 1949
BbsI GAAGAC 4 cut(s) 3552, 4352, 5499, 6053
BbvCI CCTCAGC 1 cut(s) 5583
BceAI ACGGC 3 cut(s) 982, 1461, 2729
BcgI CGANNNNNNTGC 2 cut(s) 5420, 5454
BciT130I CCWGG 7 cut(s) 538, 1406, 2062, 2958, 3624, 5600, 6075
BclI TGATCA 2 cut(s) 1380, 2796
BcnI CCSGG 1 cut(s) 2201
BcoDI GTCTC 8 cut(s) 135, 1631, 1973, 3249, 3808, 4239, 5352, 5705
BcuI ACTAGT 1 cut(s) 5476
BfmI CTRYAG 5 cut(s) 900, 1923, 3771, 5057, 5982
BfoI RGCGCY 1 cut(s) 1731
BfuAI ACCTGC 6 cut(s) 893, 1144, 2614, 3592, 4389, 5845
BglI GCCNNNNNGGC 1 cut(s) 836
BglII AGATCT 4 cut(s) 2884, 3775, 5243, 5561
BlpI GCTNAGC 2 cut(s) 478, 4785
BmcAI AGTACT 3 cut(s) 676, 3297, 5449
Bme1390I CCNGG 8 cut(s) 538, 1406, 2062, 2201, 2958, 3624, 5600, 6075
Bme18I GGWCC 6 cut(s) 471, 2106, 4571, 4631, 4853, 6234
BmeT110I CYCGRG 3 cut(s) 2220, 3524, 3644
BmiI GGNNCC 8 cut(s) 535, 2059, 3562, 4224, 4500, 4572, 5364, 6400
BmrFI CCNGG 8 cut(s) 538, 1406, 2062, 2201, 2958, 3624, 5600, 6075
BmrI ACTGGG 2 cut(s) 320, 6167
BmtI GCTAGC 1 cut(s) 5399
BmuI ACTGGG 2 cut(s) 320, 6167
BpiI GAAGAC 4 cut(s) 3552, 4352, 5499, 6053
BpmI CTGGAG 8 cut(s) 669, 729, 1655, 2613, 2847, 3432, 3645, 4482
Bpu10I CCTNAGC 5 cut(s) 3510, 4063, 4566, 5583, 6174
Bpu1102I GCTNAGC 2 cut(s) 478, 4785
BpuMI CCSGG 1 cut(s) 2201
BsaAI YACGTR 2 cut(s) 1712, 1949
BsaHI GRCGYC 1 cut(s) 5595
BsaI GGTCTC 1 cut(s) 135
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse21I CCTNAGG 1 cut(s) 1679
Bse3DI GCAATG 6 cut(s) 2085, 2661, 2940, 4560, 5205, 5661
BseBI CCWGG 7 cut(s) 538, 1406, 2062, 2958, 3624, 5600, 6075
BseMI GCAATG 6 cut(s) 2085, 2661, 2940, 4560, 5205, 5661
BseRI GAGGAG 3 cut(s) 726, 3335, 5789
BseSI GKGCMC 3 cut(s) 4654, 5476, 6402
BseYI CCCAGC 5 cut(s) 2359, 3128, 5379, 5950, 6349
BsgI GTGCAG 5 cut(s) 1560, 2820, 3330, 3570, 4359
Bsh1236I CGCG 2 cut(s) 1264, 6339
BshNI GGYRCC 3 cut(s) 533, 3560, 4498
BsiHKCI CYCGRG 3 cut(s) 2220, 3524, 3644
BsiSI CCGG 2 cut(s) 2200, 6363
BslFI GGGAC 8 cut(s) 187, 308, 1913, 2759, 3400, 4584, 6091, 6231
BsmAI GTCTC 8 cut(s) 135, 1631, 1973, 3249, 3808, 4239, 5352, 5705
BsmBI CGTCTC 1 cut(s) 1631
BsmFI GGGAC 8 cut(s) 187, 308, 1913, 2759, 3400, 4584, 6091, 6231
BsmI GAATGC 9 cut(s) 444, 969, 1054, 3943, 4093, 4267, 5052, 5800, 5850
Bso31I GGTCTC 1 cut(s) 135
BsoBI CYCGRG 3 cut(s) 2220, 3524, 3644
Bsp120I GGGCCC 1 cut(s) 6398
Bsp1407I TGTACA 2 cut(s) 229, 5553
Bsp1720I GCTNAGC 2 cut(s) 478, 4785
BspFNI CGCG 2 cut(s) 1264, 6339
BspHI TCATGA 1 cut(s) 2524
BspLI GGNNCC 8 cut(s) 535, 2059, 3562, 4224, 4500, 4572, 5364, 6400
BspMAI CTGCAG 3 cut(s) 904, 5061, 5986
BspMI ACCTGC 6 cut(s) 893, 1144, 2614, 3592, 4389, 5845
BspOI GCTAGC 1 cut(s) 5399
BspQI GCTCTTC 3 cut(s) 1431, 4806, 5163
BspT107I GGYRCC 3 cut(s) 533, 3560, 4498
BspTNI GGTCTC 1 cut(s) 135
BsrDI GCAATG 6 cut(s) 2085, 2661, 2940, 4560, 5205, 5661
BsrGI TGTACA 2 cut(s) 229, 5553
BssNAI GTATAC 2 cut(s) 3788, 4883
BssNI GRCGYC 1 cut(s) 5595
BssSI CACGAG 2 cut(s) 138, 279
BssT1I CCWWGG 8 cut(s) 526, 1105, 1117, 1656, 3969, 4980, 5907, 6183
Bst1107I GTATAC 2 cut(s) 3788, 4883
Bst2BI CACGAG 2 cut(s) 138, 279
Bst2UI CCWGG 7 cut(s) 538, 1406, 2062, 2958, 3624, 5600, 6075
Bst6I CTCTTC 9 cut(s) 345, 1431, 2263, 3480, 3829, 4242, 4806, 5013, 5163
BstACI GRCGYC 1 cut(s) 5595
BstAUI TGTACA 2 cut(s) 229, 5553
BstBAI YACGTR 2 cut(s) 1712, 1949
BstEII GGTNACC 1 cut(s) 6319
BstENI CCTNNNNNAGG 3 cut(s) 2150, 4353, 4493
BstFNI CGCG 2 cut(s) 1264, 6339
BstH2I RGCGCY 1 cut(s) 1731
BstHHI GCGC 6 cut(s) 1468, 1730, 4264, 4762, 4930, 5192
BstMAI GTCTC 8 cut(s) 135, 1631, 1973, 3249, 3808, 4239, 5352, 5705
BstNI CCWGG 7 cut(s) 538, 1406, 2062, 2958, 3624, 5600, 6075
BstNSI RCATGY 6 cut(s) 789, 1719, 2292, 3275, 4013, 5949
BstPI GGTNACC 1 cut(s) 6319
BstSCI CCNGG 8 cut(s) 536, 1404, 2060, 2199, 2956, 3622, 5598, 6073
BstSFI CTRYAG 5 cut(s) 900, 1923, 3771, 5057, 5982
BstSLI GKGCMC 3 cut(s) 4654, 5476, 6402
BstUI CGCG 2 cut(s) 1264, 6339
BstV2I GAAGAC 4 cut(s) 3552, 4352, 5499, 6053
BstXI CCANNNNNNTGG 1 cut(s) 591
BstZ17I GTATAC 2 cut(s) 3788, 4883
Bsu36I CCTNAGG 1 cut(s) 1679
BtgZI GCGATG 1 cut(s) 3368
BtsI GCAGTG 3 cut(s) 1547, 3805, 4098
BveI ACCTGC 6 cut(s) 893, 1144, 2614, 3592, 4389, 5845
CaiI CAGNNNCTG 7 cut(s) 899, 1223, 1633, 2342, 5582, 5837, 5885
CciI TCATGA 1 cut(s) 2524
CfoI GCGC 6 cut(s) 1468, 1730, 4264, 4762, 4930, 5192
CseI GACGC 4 cut(s) 200, 5189, 5462, 5584
CspCI CAANNNNNGTGG 3 cut(s) 29, 6257, 6292
DraI TTTAAA 3 cut(s) 1806, 1908, 3196
EaeI YGGCCR 3 cut(s) 925, 3357, 4937
Eam1104I CTCTTC 9 cut(s) 345, 1431, 2263, 3480, 3829, 4242, 4806, 5013, 5163
EarI CTCTTC 9 cut(s) 345, 1431, 2263, 3480, 3829, 4242, 4806, 5013, 5163
EciI GGCGGA 2 cut(s) 438, 2098
Ecl136II GAGCTC 3 cut(s) 1424, 2001, 3629
Eco130I CCWWGG 8 cut(s) 526, 1105, 1117, 1656, 3969, 4980, 5907, 6183
Eco147I AGGCCT 2 cut(s) 2156, 4432
Eco24I GRGCYC 8 cut(s) 105, 1426, 2003, 3429, 3631, 4408, 5971, 6402
Eco31I GGTCTC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 1500
Eco47I GGWCC 6 cut(s) 471, 2106, 4571, 4631, 4853, 6234
Eco47III AGCGCT 1 cut(s) 1729
Eco53kI GAGCTC 3 cut(s) 1424, 2001, 3629
Eco57I CTGAAG 5 cut(s) 1155, 2244, 2364, 5526, 5976
Eco72I CACGTG 1 cut(s) 1949
Eco81I CCTNAGG 1 cut(s) 1679
Eco88I CYCGRG 3 cut(s) 2220, 3524, 3644
Eco91I GGTNACC 1 cut(s) 6319
EcoICRI GAGCTC 3 cut(s) 1424, 2001, 3629
EcoNI CCTNNNNNAGG 3 cut(s) 2150, 4353, 4493
EcoO109I RGGNCCY 4 cut(s) 1300, 4912, 5434, 6399
EcoO65I GGTNACC 1 cut(s) 6319
EcoRI GAATTC 3 cut(s) 1010, 1321, 2627
EcoRII CCWGG 7 cut(s) 536, 1404, 2060, 2956, 3622, 5598, 6073
EcoRV GATATC 1 cut(s) 1500
EcoT14I CCWWGG 8 cut(s) 526, 1105, 1117, 1656, 3969, 4980, 5907, 6183
EcoT22I ATGCAT 3 cut(s) 200, 446, 808
EcoT38I GRGCYC 8 cut(s) 105, 1426, 2003, 3429, 3631, 4408, 5971, 6402
ErhI CCWWGG 8 cut(s) 526, 1105, 1117, 1656, 3969, 4980, 5907, 6183
Esp3I CGTCTC 1 cut(s) 1631
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 4803, 4835, 5200, 5232
FaqI GGGAC 8 cut(s) 187, 308, 1913, 2759, 3400, 4584, 6091, 6231
FauI CCCGC 3 cut(s) 939, 3416, 6389
FauNDI CATATG 2 cut(s) 1090, 3212
FbaI TGATCA 2 cut(s) 1380, 2796
FblI GTMKAC 3 cut(s) 463, 3787, 4882
FriOI GRGCYC 8 cut(s) 105, 1426, 2003, 3429, 3631, 4408, 5971, 6402
FspI TGCGCA 1 cut(s) 4263
GlaI GCGC 6 cut(s) 1467, 1729, 4263, 4761, 4929, 5191
GsaI CCCAGC 5 cut(s) 2363, 3132, 5383, 5954, 6353
GsuI CTGGAG 8 cut(s) 669, 729, 1655, 2613, 2847, 3432, 3645, 4482
HaeII RGCGCY 1 cut(s) 1731
HapII CCGG 2 cut(s) 2200, 6363
HgaI GACGC 4 cut(s) 200, 5189, 5462, 5584
HhaI GCGC 6 cut(s) 1468, 1730, 4264, 4762, 4930, 5192
Hin1I GRCGYC 1 cut(s) 5595
Hin6I GCGC 6 cut(s) 1466, 1728, 4262, 4760, 4928, 5190
HinP1I GCGC 6 cut(s) 1466, 1728, 4262, 4760, 4928, 5190
HincII GTYRAC 5 cut(s) 1863, 3845, 4197, 4669, 4710
HindII GTYRAC 5 cut(s) 1863, 3845, 4197, 4669, 4710
HindIII AAGCTT 5 cut(s) 857, 879, 3910, 5934, 6223
HpaII CCGG 2 cut(s) 2200, 6363
Hpy99I CGWCG 1 cut(s) 5183
HpyCH4IV ACGT 5 cut(s) 768, 1711, 1948, 4077, 5843
HpySE526I ACGT 5 cut(s) 768, 1711, 1948, 4077, 5843
Hsp92I GRCGYC 1 cut(s) 5595
HspAI GCGC 6 cut(s) 1466, 1728, 4262, 4760, 4928, 5190
Ksp22I TGATCA 2 cut(s) 1380, 2796
LguI GCTCTTC 3 cut(s) 1431, 4806, 5163
MaeII ACGT 5 cut(s) 768, 1711, 1948, 4077, 5843
MfeI CAATTG 5 cut(s) 1974, 2418, 2781, 4017, 5976
MlsI TGGCCA 3 cut(s) 927, 3359, 4939
MluNI TGGCCA 3 cut(s) 927, 3359, 4939
MlyI GAGTC 5 cut(s) 3382, 3649, 3815, 4831, 6253
MmeI TCCRAC 5 cut(s) 283, 340, 529, 4266, 5589
Mox20I TGGCCA 3 cut(s) 927, 3359, 4939
Mph1103I ATGCAT 3 cut(s) 200, 446, 808
MroXI GAANNNNTTC 3 cut(s) 1485, 3877, 6304
MscI TGGCCA 3 cut(s) 927, 3359, 4939
MslI CAYNNNNRTG 2 cut(s) 1750, 5253
Msp20I TGGCCA 3 cut(s) 927, 3359, 4939
MspA1I CMGCKG 7 cut(s) 208, 500, 2213, 4784, 5039, 5954, 5987
MspI CCGG 2 cut(s) 2200, 6363
MspR9I CCNGG 8 cut(s) 538, 1406, 2062, 2201, 2958, 3624, 5600, 6075
MunI CAATTG 5 cut(s) 1974, 2418, 2781, 4017, 5976
Mva1269I GAATGC 9 cut(s) 444, 969, 1054, 3943, 4093, 4267, 5052, 5800, 5850
MvaI CCWGG 7 cut(s) 538, 1406, 2062, 2958, 3624, 5600, 6075
MvnI CGCG 2 cut(s) 1264, 6339
NciI CCSGG 1 cut(s) 2201
NdeI CATATG 2 cut(s) 1090, 3212
NheI GCTAGC 1 cut(s) 5395
NlaIV GGNNCC 8 cut(s) 535, 2059, 3562, 4224, 4500, 4572, 5364, 6400
NmeAIII GCCGAG 1 cut(s) 4180
NmuCI GTSAC 6 cut(s) 3092, 4243, 4794, 4834, 4966, 5174
NsbI TGCGCA 1 cut(s) 4263
NsiI ATGCAT 3 cut(s) 200, 446, 808
NspI RCATGY 6 cut(s) 789, 1719, 2292, 3275, 4013, 5949
PaeI GCATGC 2 cut(s) 4013, 5949
PaeR7I CTCGAG 2 cut(s) 2220, 3524
PagI TCATGA 1 cut(s) 2524
PaqCI CACCTGC 2 cut(s) 893, 1144
PasI CCCWGGG 1 cut(s) 2957
PceI AGGCCT 2 cut(s) 2156, 4432
PciI ACATGT 1 cut(s) 2288
PciSI GCTCTTC 3 cut(s) 1431, 4806, 5163
PctI GAATGC 9 cut(s) 444, 969, 1054, 3943, 4093, 4267, 5052, 5800, 5850
PdmI GAANNNNTTC 3 cut(s) 1485, 3877, 6304
PflFI GACNNNGTC 1 cut(s) 5881
PflMI CCANNNNNTGG 1 cut(s) 4628
PfoI TCCNGGA 3 cut(s) 1404, 2060, 3622
PleI GAGTC 5 cut(s) 3382, 3649, 3815, 4831, 6252
PmaCI CACGTG 1 cut(s) 1949
PmlI CACGTG 1 cut(s) 1949
PpsI GAGTC 5 cut(s) 3382, 3649, 3815, 4831, 6252
Ppu21I YACGTR 2 cut(s) 1712, 1949
PscI ACATGT 1 cut(s) 2288
PshBI ATTAAT 1 cut(s) 2520
PsiI TTATAA 1 cut(s) 125
Psp124BI GAGCTC 3 cut(s) 1426, 2003, 3631
Psp1406I AACGTT 1 cut(s) 5843
Psp6I CCWGG 7 cut(s) 536, 1404, 2060, 2956, 3622, 5598, 6073
PspCI CACGTG 1 cut(s) 1949
PspEI GGTNACC 1 cut(s) 6319
PspFI CCCAGC 5 cut(s) 2359, 3128, 5379, 5950, 6349
PspGI CCWGG 7 cut(s) 536, 1404, 2060, 2956, 3622, 5598, 6073
PspN4I GGNNCC 8 cut(s) 535, 2059, 3562, 4224, 4500, 4572, 5364, 6400
PspOMI GGGCCC 1 cut(s) 6398
PstI CTGCAG 3 cut(s) 904, 5061, 5986
PstNI CAGNNNCTG 7 cut(s) 899, 1223, 1633, 2342, 5582, 5837, 5885
PsyI GACNNNGTC 1 cut(s) 5881
PvuII CAGCTG 6 cut(s) 208, 500, 2213, 4784, 5954, 5987
RseI CAYNNNNRTG 2 cut(s) 1750, 5253
SacI GAGCTC 3 cut(s) 1426, 2003, 3631
SapI GCTCTTC 3 cut(s) 1431, 4806, 5163
ScaI AGTACT 3 cut(s) 676, 3297, 5449
SchI GAGTC 5 cut(s) 3382, 3649, 3815, 4831, 6253
ScrFI CCNGG 8 cut(s) 538, 1406, 2062, 2201, 2958, 3624, 5600, 6075
SfcI CTRYAG 5 cut(s) 900, 1923, 3771, 5057, 5982
Sfr274I CTCGAG 2 cut(s) 2220, 3524
SinI GGWCC 6 cut(s) 471, 2106, 4571, 4631, 4853, 6234
SlaI CTCGAG 2 cut(s) 2220, 3524
SmiMI CAYNNNNRTG 2 cut(s) 1750, 5253
SpeI ACTAGT 1 cut(s) 5476
SphI GCATGC 2 cut(s) 4013, 5949
SseBI AGGCCT 2 cut(s) 2156, 4432
SspI AATATT 5 cut(s) 561, 1072, 2178, 2706, 4542
SstI GAGCTC 3 cut(s) 1426, 2003, 3631
StuI AGGCCT 2 cut(s) 2156, 4432
StyD4I CCNGG 8 cut(s) 536, 1404, 2060, 2199, 2956, 3622, 5598, 6073
StyI CCWWGG 8 cut(s) 526, 1105, 1117, 1656, 3969, 4980, 5907, 6183
TaiI ACGT 5 cut(s) 771, 1714, 1951, 4080, 5846
TaqI TCGA 9 cut(s) 346, 1722, 2186, 2221, 2880, 3525, 3952, 4213, 5443
TauI GCSGC 4 cut(s) 2174, 4211, 5039, 5654
TseFI GTSAC 6 cut(s) 3092, 4243, 4794, 4834, 4966, 5174
Tsp45I GTSAC 6 cut(s) 3092, 4243, 4794, 4834, 4966, 5174
TspGWI ACGGA 4 cut(s) 799, 1794, 4268, 6421
Tth111I GACNNNGTC 1 cut(s) 5881
Van91I CCANNNNNTGG 1 cut(s) 4628
VneI GTGCAC 2 cut(s) 4650, 5472
VpaK11BI GGWCC 6 cut(s) 471, 2106, 4571, 4631, 4853, 6234
VspI ATTAAT 1 cut(s) 2520
XagI CCTNNNNNAGG 3 cut(s) 2150, 4353, 4493
XapI RAATTY 8 cut(s) 1010, 1321, 2049, 2627, 3406, 5072, 6053, 6409
XbaI TCTAGA 1 cut(s) 2509
XceI RCATGY 6 cut(s) 789, 1719, 2292, 3275, 4013, 5949
XhoI CTCGAG 2 cut(s) 2220, 3524
XmiI GTMKAC 3 cut(s) 463, 3787, 4882
XmnI GAANNNNTTC 3 cut(s) 1485, 3877, 6304
ZrmI AGTACT 3 cut(s) 676, 3297, 5449
Zsp2I ATGCAT 3 cut(s) 200, 446, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.