MD01G1024000.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007148
Physical Location & Seq
Forward (+)
9109994 .. 9119637
9644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1024000.v1.1.491

Sequence Viewer

Length: 6435 bp
ATGGCAACCACAATGGGATGGAGGTATGCTGCCACCAATGGCAGCACCCTTTCCACTAATGATCTGGAAAGGAATGGTGATGCAAAAGTTCAAGATTCGGAGCCTCCAACACCACATTCTCTTTTAAAGATGGGTTCACGAGATCGTAGTAGTAGCATGGAGGATGCAGATGGGACATTGGCAAGTGTTGCTCAATGCATTGAGCAGCTGCGTCAAAGTTCCTCATCTGTACAAGACAAGGAGTATTCGTTGAAGCAGTTGCTTGAGCTTATCAATACTCGTGAAAATGCTTTCAGTGCTGTTGGATCTCACTCTCAAGCAGTTCCAGTGCTTGTTTCTCTTCTTCGATCAGGTTCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTCTCTATGCAAGGAGAATGAACTCAGGGTGAAAGTCCTGCTTGGAGGATGCATTCCGCCATTGCTTGGTCTACTCAGGTCCAGCTCAGCAGAAGGGCAAGTTGCATCTGCGAAGACAATTTATGCTGTTTCTCAAGGTGGTGCCAGGGATCATGTTGGATCAAAAATATTTTCAACTGAAGGAGTTGTGCCAGTGCTCTGGGAGCAGCTACAAAAAGGGATAAAAACTGGTAGTTTGGTTGATAGTTTGTTGACTGGAGCATTAAAGAACCTATCCAGCAGCACTGAGGGATTCTGGACTGCAACCTTCCAAGCTGGAGCAGTGGATATACTTGTGAAGTTGCTGACAACTGGGCAGTCAAGCACTCAAGCTAACGTGTGCTTTCTACTTGCATGTATGATGGTGGAAGATCCATCTGTCTGTTCTAAGGTGCTGGCTTCAGAGGTTACCAAACAACTTCTCAAGCTTTTAGGATCCGGAAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTCTTAAATCTCTTTCTGCCCAGTGCAAAGAAGCAAGACGGGAAATAGCTAACTTCAACGGCATACCTGTATTGATAAATGCTACAATAGCCCCTTCCAAAGAATTCATGCAGGGTGAGCATGCCCAAGCATTGCAGGAGAATGCCATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTTATCTCAAGCCTTGGGCAAAGCCTTGAATCTTGCACTTCACCTGCCCAAATTTCTGATACATTAGGGGCTTTGGCTTCAGCTTTGATGATATATGATAGTACGGCAGAATCTAATAGAGCATCAGATCCTGTGGTTATTGAGCAGACATTGGTTTCACAGTTCAAACCTCGTTTACCGTTTCTTGTTCAGGAACGAACCATAGAGGCCCTTGCGAGTTTGTATGGGAATTCTGTACTCTCAGTTAAACTTGCCAATTCAGAAGCAAAGCGTTTGCTTGTTGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTAATGAGAGCTCTTCTTGCACTTTGCAATAGTGAGGAAAGTCTGTGGCGTGCACTTCAAGGCCGTGAAGGGGTTCAGCTGTTGATATCACTTCTTGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTGCTTTCCAATGAGAATGACGAAAGTAAATGGGCTATTACTGCTGCTGGTGGCATACCTCCACTTGTTCAGATTCTGGAGACAGGATCTGCAAAAGCCAAGGAAGATTCAGCATCAATCCTTAGGAACCTGTGCAACCACAGTGAAGATATACGTGCATGTGTCGAAAGTGCTGATGCTGTTCCTGCATTACTGTGGCTTCTAAAGAATGGAAGTTCAAACGGAAAAGAAATTGCAGCAAAGACTTTAAATCATTTGATCCATAAATCTGATACAGCAACCATTAGCCAACTTACTGCCTTATTGACCAGTGATCTACCTGAATCTAAAGTGTATGTTTTGGATGCTTTAAAAAGTATGCTGTCTGTGGTCCCCCTCAATGATATATCACGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATAAAAATATTGAGCTCAACTAAAGAAGAGACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATATTTGAATCCAGGAAGGACTTGCGTGAAGGTAGCATTGCTGTTAAAACTCTTTGGTCAGCCATTAAGTTGATTAGTGTTGAATCTGTATATATCCTTGCAGAGGCCTCACGTTGCCTTGCGGCAATATTTCTTTCGATTAAAGAGAACCGGGATGTGGCAGCTGTTGCTCGAGATATATTATCTCCGTTAGTTGTGCTTGCTAACTCTTCAGTTCTGGAAGTTGCAGAACTAGCAACATGTGCTCTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAGAAAGCAGTTGCTGAAGATATTATTTTCCCGGCTACTAGAGTTTTGCGTGAAGGCACAGTACCTGGAAAGACTCATGCAGCAGCAGCAATTGCTCGCCTGCTCCATTCTCGTCAAATTGATTATGCTTTAACTGATTGTGTGAATTGTGCTGGAACTGTTCTTGCATTAGTTTCTTTTCTAGAATCTGTTAATCATGATTCTGTTGCCACATTAGAGGCACTTGAGGCACTTGCTATTCTATCCAGGTCAGAAGGTGCTACTGGAGATATAAAACCTGCATGGGCAGTTTTAGCCGAATTCCCGAAAAGCATTACCCCAATAGTTTTATCCATTGCTGATGCAACACCCTTGTTGCAGGATAAGGCTATTGAAATATTATCACGGCTTTGCAGAGATCAGCCTGATGTATTGGGTGACACGGTTGCTACTGCTTATGGATGTATTTCATCAATTGCCAAAAGAGTGATCAATTCAACGAAATCAAAAGTTAAAACTGGAGGAACTGCACTACTTATTTGTGTTGCAAAAGTTAGTCATCAGAGAATTGTGGAAGATCTTAGTGAATCAAACTTATGCACCCAGCTAATTCAAGCTCTTGTTGCAATGTTTAGTTCTTTGGGAAGCCCAGGGGATAATGAGAATGATTCTATTGGCATTTATAGGCATTCTAAAGAGGAAACAAAGATTGGTGAGTCCACAGGAGTAATTAGTGGTGTCAATTTAGCTATGTGGCTACTGTCTATTCTTGCCTGTCACGATGAAAGGTGCAAAATTGTGATTATGGAGGCTGGGGCTGTGGAAGTCCTCACTGACAGGATCTCAAATGATTTCTCACCCTACTCACAGATTGAGTTTAAAGAAGATAGTAGTATATGGATTTGTACTTTGCTGCTAGCAATCTTGTTACAAAACAGAGACATCATACGAGCACATGCAACCATGAAATCTGTACCAGTACTTGCAAACTGGTTGAGATCAGAGGATTTGCCCACCAGATATTTTGCTGCACAGGCGATGGCCAGCCTAGTCTGTAATGGTAGCAGGGGGACTCTTCTATCTGTTGCAAATTCTGGAGCAGCAGGCGGGCTCATTTCCCTACTTGGCTGTGCAGATATTGATATAAGTGATCTTTTGCAGTTGTCAGAAGAGTGTGGTTTAGTGCGTTATCCTGAGCAAGTTGCCCTTGAGAGGTTGTTCAGAGTTGAAGACATAAGGGTTGGCGCTACTTCCAGGAAAGCAATACCTGCACTTGTTGATCTACTCAAACCCATTCCAGATCGTCCTGGAGCACCATTTTTAGCACTTGGGCTTCTGACTCAGCTTGCGAAAGACTGTCCGTCGAATAAAATTGTAATGGTAGAATCAGGAGCTTTGGAAGCACTGACCAAATATCTTTCACTTGGCCCTCAAGATGCAACTGAGGAAGCTGCTACAGATCTGTTAGGTATACTATTTGGCAGTGCTGAAATCAGGAGACATGACTCTTCATTTGGTGCTGTTGGTCAGCTTGTGGCAGTTTTACGTTTAGGTGGAAGAGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTGTTTTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAAGCTGTTCAACCATTGGTGGAGATTCTCAATACTGGTTCTGAGAAGGAGCAACAAGCTGCAATTGCTGCGTTGGTAAGGCTGCTGAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGATGTTGAGATGAATGCAGTAGATGTTCTCTGCAAGATTCTTTCTTCAAATTGCTCAATGGAGCTGAAAGGGGAAGCTGCCGAGTTGTGTTGTGTTCTCTTTGGAAATACAAGAATCAGGTCAACAATGGCTGCAGCTAGATGTGTGGAGCCTCTGGTGTCTCTTCTTGTGACCGAGTTCAGTCCTGCACAGCATTCAGTTGTCCGTGCATTGGATAAACTTGTTGATGATGAGCAACTGGCAGAACTTGTTGCTGCTCACGGGGCAGTTATTCCTCTTGTAGGTCTTCTATATGGTAAGAATTACTTGCTCCATGAAGCTATTTCCAGAGCCCTTGTGAAATTAGGGAAAGACAGGCCTGCTTGCAAGATGGAAATGGTGAAAGCTGGAGTAATTGAGAGCATACTTGACATCCTCCATGAGGCACCCGATTTTCTCTCTGCTGCTTTTGCAGAACTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGACCGTCTGCTTCAAAAGTGGTTGAGCCCCTTTTTGTGCTTCTAACAAGACCAGAGTTTGGACCTGATGGGCAGCATAGTGCACTTCAAGTTCTTGTCAACGTTTTGGAGCATCCACAGTGTCGTTCTGACTATAAGTTGACTTCCCACCAAGCTGTTGAACCTATTATTCCCTTACTTGATTCTCCTGCTCCAGCAGTACAACAGTTGGCCACTGAGCTTCTGTCACATTTACTCTTTGAAGAACAACTTCAAAAGGATTCAGTGACTCAGCAAGTGATTGGTCCTCTCATACGGGTTCTTGGCTCTGGTATACACATTTTGCAGCAGAGAGCTGTGAAGGCCCTTGTTAGTATTGCGCTAATTTGGCCGAATGAAATAGCAAAAGAGGGTGGTGTCACTGAATTGTCCAAGGTGATATTGCAATCAGATACATCTCTTCCTCATGCCTTGTGGGAATCGGCTGCTGCTGTTTTATCAAGCATTCTGCAATTTAGTTCTGAATTTTATTTGGAAGTGCCTGTGGCTGTGTTGGTAAGGTTGCTTCGTTCTGGCTCAGAAGGCACAGTAATTGGTGCATTGAATGCTCTTCTGGTACTGGAAAGTGATGATGCAACCAGTGCAGAAGCAATGGCTGAAAGTGGTGCTTTAGAGGCTCTTTTGGATCTTCTTAGATCTCATCAGTGTGAGGAAACTGCTGCTAGGCTGTTGGAAGTACTGTTGAACAATGTGAAGATCAGAGAAACCAAGGCTACCAAGTCTGCCGTATTACCATTGTCTCAGTACCTCTTAGATCCACAAACCCAAGCTCAGCAAGCGAGGTTACTGGCAACTCTAGCTCTTGGTGATCTATTCCAGAATGAGGGCCTTGCTCGAAGCACTGACGCTGTTTCAGCTTGTCGTGCACTAGTAAATGTGCTTGAAGAGCAACCTACTGAAGAAATGAAGGTTGTAGCGATATGTGCATTGCAGAACCTTGTCATGTACAGTAGATCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGCGTCCAGGTTGTGTTGGATCTGATTGGTTCAAGCGATCCAGATACTTCTATTCAGGCTGCAATGTTTGTTAAACTTCTCTTTTCAAACCATACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCGATTACTGCTGCTATCGAGAAAGATTTATGGGCAACCGGAACTGTGAATGAGGAGTACCTGAAAGCTCTGAACGCTCTTTTTAGCAACTTCCCACGACTTAGAGCCACAGAACCTGCAACGTTAAGCATTCCTCATCTTGTTACATCCCTCAAGACAGGGTCAGAGGCAACTCAAGAAGCTGCCTTGGATGCACTCTTTCTTCTTAGGCAAGCTTGGTCAGCATGTCCAGCTGAAGTTTCTAGGGCGCAGTCAATTGCTGCAGCCGATGCAATCCCCTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGGTTTCAGGAGAAGACAGAATTTTTGTTACAATGTTTGCCGGGGACATTGGTGGTGATAATCAAGCGTGGAAATAATATGAAGCAGTCAGTGGGAAATCCTAGTGTTTATTGCAAGATTACACTCGGCAACACTCCACCTAAGCAAACTCAGGTTGTTTCAACTGGACCGAATCCCGAATGGGATGAAAGCTTTTTGTGGTCCTTTGAGAGTCCTCCGAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAAATGGGAAAGAGTTCATTTGGAAAGGTAACCATCCAGATTGATCGTGTAGTAATGCTGGGAGCAGTTGCCGGCGAGTACACTCTGTTGCCAGAAAGCAAAAGCGGGCCGTCACGGAATTTAGAAATAGAGTTCCAGTGGTCTAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2145

Amino Acids

229.17

Weight (kDa)

5.3

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 62 - 313 2.8e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 427 - 604 8.2e-10 ARMC5
C2 PF00168 2023 - 2117 3.8e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 893, 1144
Acc36I ACCTGC 5 cut(s) 893, 1144, 2614, 3583, 5836
AccB1I GGYRCC 2 cut(s) 533, 4489
AccB7I CCANNNNNTGG 2 cut(s) 458, 4619
AccI GTMKAC 3 cut(s) 463, 3778, 4873
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 4 cut(s) 449, 2171, 3414, 6387
AclI AACGTT 2 cut(s) 4662, 5834
AcoI YGGCCR 3 cut(s) 3348, 4770, 4928
AcsI RAATTY 8 cut(s) 1010, 1143, 1321, 2627, 3397, 5063, 6044, 6400
AcuI CTGAAG 7 cut(s) 591, 816, 1155, 2244, 2364, 5517, 5967
AcvI CACGTG 1 cut(s) 1949
AcyI GRCGYC 1 cut(s) 5586
AflIII ACRYGT 2 cut(s) 768, 2288
AhdI GACNNNNNGTC 1 cut(s) 3667
AhlI ACTAGT 1 cut(s) 5467
AjnI CCWGG 8 cut(s) 536, 2060, 2392, 2573, 2956, 3560, 3613, 5589
AjuI GAANNNNNNNTTGG 4 cut(s) 100, 132, 3000, 3032
Alw26I GTCTC 8 cut(s) 1631, 1973, 2009, 3240, 3799, 4230, 5343, 5696
Alw44I GTGCAC 3 cut(s) 1464, 4641, 5463
AlwNI CAGNNNCTG 8 cut(s) 899, 1223, 1633, 1646, 2342, 2393, 5573, 5828
Ama87I CYCGRG 1 cut(s) 2220
Aor13HI TCCGGA 1 cut(s) 869
ApaLI GTGCAC 3 cut(s) 1464, 4641, 5463
ApoI RAATTY 8 cut(s) 1010, 1143, 1321, 2627, 3397, 5063, 6044, 6400
ArsI GACNNNNNNTTYG 4 cut(s) 3803, 3835, 6031, 6063
Asp700I GAANNNNTTC 4 cut(s) 1485, 3868, 4809, 6295
AspLEI GCGC 3 cut(s) 3554, 4921, 5962
AsuC2I CCSGG 3 cut(s) 2201, 2360, 6066
AsuNHI GCTAGC 1 cut(s) 3223
AvaI CYCGRG 1 cut(s) 2220
AvaII GGWCC 7 cut(s) 471, 1927, 4562, 4622, 4844, 6191, 6225
AxyI CCTNAGG 1 cut(s) 1679
BaeGI GKGCMC 3 cut(s) 1468, 4645, 5467
BalI TGGCCA 2 cut(s) 3350, 4772
BamHI GGATCC 1 cut(s) 866
BanI GGYRCC 2 cut(s) 533, 4489
BanII GRGCYC 5 cut(s) 1426, 2003, 3420, 4399, 4590
BarI GAAGNNNNNNTAC 4 cut(s) 2373, 2405, 5088, 5120
BauI CACGAG 2 cut(s) 138, 279
BbrPI CACGTG 1 cut(s) 1949
BbsI GAAGAC 4 cut(s) 512, 3543, 4343, 6044
BbvCI CCTCAGC 1 cut(s) 5574
BceAI ACGGC 6 cut(s) 982, 1212, 1461, 2729, 5309, 6376
BciT130I CCWGG 8 cut(s) 538, 2062, 2394, 2575, 2958, 3562, 3615, 5591
BclI TGATCA 2 cut(s) 1380, 2796
BcnI CCSGG 3 cut(s) 2201, 2360, 6066
BcoDI GTCTC 8 cut(s) 1631, 1973, 2009, 3240, 3799, 4230, 5343, 5696
BcuI ACTAGT 1 cut(s) 5467
BfmI CTRYAG 4 cut(s) 900, 3762, 4197, 5973
BfoI RGCGCY 1 cut(s) 3555
BfuAI ACCTGC 5 cut(s) 893, 1144, 2614, 3583, 5836
BglII AGATCT 4 cut(s) 2884, 3766, 5234, 5552
BlpI GCTNAGC 2 cut(s) 478, 5370
BmcAI AGTACT 2 cut(s) 3288, 5277
Bme18I GGWCC 7 cut(s) 471, 1927, 4562, 4622, 4844, 6191, 6225
BmeRI GACNNNNNGTC 1 cut(s) 3667
BmeT110I CYCGRG 1 cut(s) 2220
BmiI GGNNCC 8 cut(s) 102, 535, 868, 1685, 1929, 4215, 4491, 4563
BmrI ACTGGG 2 cut(s) 753, 922
BmtI GCTAGC 1 cut(s) 3227
BmuI ACTGGG 2 cut(s) 753, 922
BpiI GAAGAC 4 cut(s) 512, 3543, 4343, 6044
BpmI CTGGAG 9 cut(s) 669, 729, 1655, 2613, 2847, 3423, 3636, 4473, 4737
Bpu10I CCTNAGC 5 cut(s) 3501, 4054, 4557, 5574, 6165
Bpu1102I GCTNAGC 2 cut(s) 478, 5370
BpuMI CCSGG 3 cut(s) 2201, 2360, 6066
BsaAI YACGTR 2 cut(s) 1712, 1949
BsaHI GRCGYC 1 cut(s) 5586
BsaWI WCCGGW 2 cut(s) 869, 5750
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse118I RCCGGY 1 cut(s) 6353
Bse21I CCTNAGG 1 cut(s) 1679
Bse3DI GCAATG 9 cut(s) 452, 1037, 2085, 2661, 2940, 4551, 5196, 5525, 5652
BseAI TCCGGA 1 cut(s) 869
BseBI CCWGG 8 cut(s) 538, 2062, 2394, 2575, 2958, 3562, 3615, 5591
BseMI GCAATG 9 cut(s) 452, 1037, 2085, 2661, 2940, 4551, 5196, 5525, 5652
BseRI GAGGAG 1 cut(s) 5780
BseSI GKGCMC 3 cut(s) 1468, 4645, 5467
BseYI CCCAGC 3 cut(s) 2910, 3119, 6340
BsgI GTGCAG 7 cut(s) 1560, 2820, 3321, 3459, 3561, 4236, 5202
BshNI GGYRCC 2 cut(s) 533, 4489
BsiHKCI CYCGRG 1 cut(s) 2220
BsiSI CCGG 6 cut(s) 870, 2200, 2360, 5751, 6065, 6354
BslFI GGGAC 5 cut(s) 187, 1913, 3391, 4575, 6082
BsmAI GTCTC 8 cut(s) 1631, 1973, 2009, 3240, 3799, 4230, 5343, 5696
BsmFI GGGAC 5 cut(s) 187, 1913, 3391, 4575, 6082
BsmI GAATGC 9 cut(s) 444, 1054, 2995, 3934, 4084, 4258, 5043, 5149, 5841
BsoBI CYCGRG 1 cut(s) 2220
Bsp13I TCCGGA 1 cut(s) 869
Bsp1407I TGTACA 2 cut(s) 229, 5544
Bsp1720I GCTNAGC 2 cut(s) 478, 5370
BspACI CCGC 4 cut(s) 449, 2171, 3414, 6387
BspEI TCCGGA 1 cut(s) 869
BspHI TCATGA 1 cut(s) 2524
BspLI GGNNCC 8 cut(s) 102, 535, 868, 1685, 1929, 4215, 4491, 4563
BspMAI CTGCAG 3 cut(s) 904, 4201, 5977
BspMI ACCTGC 5 cut(s) 893, 1144, 2614, 3583, 5836
BspOI GCTAGC 1 cut(s) 3227
BspQI GCTCTTC 4 cut(s) 1431, 3859, 5154, 5478
BspT107I GGYRCC 2 cut(s) 533, 4489
BsrDI GCAATG 9 cut(s) 452, 1037, 2085, 2661, 2940, 4551, 5196, 5525, 5652
BsrFI RCCGGY 1 cut(s) 6353
BsrGI TGTACA 2 cut(s) 229, 5544
BssAI RCCGGY 1 cut(s) 6353
BssNAI GTATAC 2 cut(s) 3779, 4874
BssNI GRCGYC 1 cut(s) 5586
BssSI CACGAG 2 cut(s) 138, 279
BssT1I CCWWGG 5 cut(s) 1105, 1656, 4971, 5307, 5898
Bst1107I GTATAC 2 cut(s) 3779, 4874
Bst2BI CACGAG 2 cut(s) 138, 279
Bst2UI CCWGG 8 cut(s) 538, 2062, 2394, 2575, 2958, 3562, 3615, 5591
BstACI GRCGYC 1 cut(s) 5586
BstAUI TGTACA 2 cut(s) 229, 5544
BstBAI YACGTR 2 cut(s) 1712, 1949
BstEII GGTNACC 2 cut(s) 838, 6310
BstENI CCTNNNNNAGG 4 cut(s) 2150, 4344, 4484, 6243
BstH2I RGCGCY 1 cut(s) 3555
BstHHI GCGC 3 cut(s) 3554, 4921, 5962
BstMAI GTCTC 8 cut(s) 1631, 1973, 2009, 3240, 3799, 4230, 5343, 5696
BstNI CCWGG 8 cut(s) 538, 2062, 2394, 2575, 2958, 3562, 3615, 5591
BstNSI RCATGY 6 cut(s) 789, 1031, 1719, 2292, 3266, 5940
BstPI GGTNACC 2 cut(s) 838, 6310
BstSFI CTRYAG 4 cut(s) 900, 3762, 4197, 5973
BstSLI GKGCMC 3 cut(s) 1468, 4645, 5467
BstV2I GAAGAC 4 cut(s) 512, 3543, 4343, 6044
BstXI CCANNNNNNTGG 1 cut(s) 591
BstZ17I GTATAC 2 cut(s) 3779, 4874
Bsu36I CCTNAGG 1 cut(s) 1679
BtgZI GCGATG 1 cut(s) 3359
BtsI GCAGTG 3 cut(s) 720, 1547, 3796
BveI ACCTGC 5 cut(s) 893, 1144, 2614, 3583, 5836
CaiI CAGNNNCTG 8 cut(s) 899, 1223, 1633, 1646, 2342, 2393, 5573, 5828
CciI TCATGA 1 cut(s) 2524
CfoI GCGC 3 cut(s) 3554, 4921, 5962
Cfr10I RCCGGY 1 cut(s) 6353
CseI GACGC 3 cut(s) 200, 5453, 5575
CspCI CAANNNNNGTGG 2 cut(s) 6248, 6283
DraI TTTAAA 4 cut(s) 126, 1806, 1908, 3187
DriI GACNNNNNGTC 1 cut(s) 3667
EaeI YGGCCR 3 cut(s) 3348, 4770, 4928
Eam1105I GACNNNNNGTC 1 cut(s) 3667
EciI GGCGGA 1 cut(s) 438
Ecl136II GAGCTC 2 cut(s) 1424, 2001
Eco130I CCWWGG 5 cut(s) 1105, 1656, 4971, 5307, 5898
Eco147I AGGCCT 2 cut(s) 2156, 4423
Eco24I GRGCYC 5 cut(s) 1426, 2003, 3420, 4399, 4590
Eco32I GATATC 1 cut(s) 1500
Eco47I GGWCC 7 cut(s) 471, 1927, 4562, 4622, 4844, 6191, 6225
Eco53kI GAGCTC 2 cut(s) 1424, 2001
Eco57I CTGAAG 7 cut(s) 591, 816, 1155, 2244, 2364, 5517, 5967
Eco72I CACGTG 1 cut(s) 1949
Eco81I CCTNAGG 1 cut(s) 1679
Eco88I CYCGRG 1 cut(s) 2220
Eco91I GGTNACC 2 cut(s) 838, 6310
EcoICRI GAGCTC 2 cut(s) 1424, 2001
EcoNI CCTNNNNNAGG 4 cut(s) 2150, 4344, 4484, 6243
EcoO109I RGGNCCY 3 cut(s) 1300, 4903, 5425
EcoO65I GGTNACC 2 cut(s) 838, 6310
EcoRI GAATTC 3 cut(s) 1010, 1321, 2627
EcoRII CCWGG 8 cut(s) 536, 2060, 2392, 2573, 2956, 3560, 3613, 5589
EcoRV GATATC 1 cut(s) 1500
EcoT14I CCWWGG 5 cut(s) 1105, 1656, 4971, 5307, 5898
EcoT22I ATGCAT 2 cut(s) 200, 446
EcoT38I GRGCYC 5 cut(s) 1426, 2003, 3420, 4399, 4590
ErhI CCWWGG 5 cut(s) 1105, 1656, 4971, 5307, 5898
FaqI GGGAC 5 cut(s) 187, 1913, 3391, 4575, 6082
FauI CCCGC 2 cut(s) 3407, 6380
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 2 cut(s) 1380, 2796
FblI GTMKAC 3 cut(s) 463, 3778, 4873
FriOI GRGCYC 5 cut(s) 1426, 2003, 3420, 4399, 4590
GlaI GCGC 3 cut(s) 3553, 4920, 5961
GsaI CCCAGC 3 cut(s) 2914, 3123, 6344
GsuI CTGGAG 9 cut(s) 669, 729, 1655, 2613, 2847, 3423, 3636, 4473, 4737
HaeII RGCGCY 1 cut(s) 3555
HapII CCGG 6 cut(s) 870, 2200, 2360, 5751, 6065, 6354
HgaI GACGC 3 cut(s) 200, 5453, 5575
HhaI GCGC 3 cut(s) 3554, 4921, 5962
Hin1I GRCGYC 1 cut(s) 5586
Hin6I GCGC 3 cut(s) 3552, 4919, 5960
HinP1I GCGC 3 cut(s) 3552, 4919, 5960
HincII GTYRAC 4 cut(s) 645, 4188, 4660, 4701
HindII GTYRAC 4 cut(s) 645, 4188, 4660, 4701
HindIII AAGCTT 5 cut(s) 857, 879, 3901, 5925, 6214
HpaII CCGG 6 cut(s) 870, 2200, 2360, 5751, 6065, 6354
Hpy99I CGWCG 1 cut(s) 3673
HpyCH4IV ACGT 7 cut(s) 768, 1711, 1948, 2161, 3853, 4662, 5834
HpySE526I ACGT 7 cut(s) 768, 1711, 1948, 2161, 3853, 4662, 5834
Hsp92I GRCGYC 1 cut(s) 5586
HspAI GCGC 3 cut(s) 3552, 4919, 5960
Kpn2I TCCGGA 1 cut(s) 869
KroI GCCGGC 1 cut(s) 6353
KroNI GCCGGC 1 cut(s) 6355
Ksp22I TGATCA 2 cut(s) 1380, 2796
LguI GCTCTTC 4 cut(s) 1431, 3859, 5154, 5478
MaeII ACGT 7 cut(s) 768, 1711, 1948, 2161, 3853, 4662, 5834
MfeI CAATTG 5 cut(s) 1974, 2418, 2781, 4008, 5967
MlsI TGGCCA 2 cut(s) 3350, 4772
MluNI TGGCCA 2 cut(s) 3350, 4772
MlyI GAGTC 8 cut(s) 2011, 2395, 3032, 3373, 3640, 3806, 4822, 6244
MmeI TCCRAC 6 cut(s) 131, 283, 340, 529, 5250, 5580
Mox20I TGGCCA 2 cut(s) 3350, 4772
Mph1103I ATGCAT 2 cut(s) 200, 446
MroI TCCGGA 1 cut(s) 869
MroNI GCCGGC 1 cut(s) 6353
MroXI GAANNNNTTC 4 cut(s) 1485, 3868, 4809, 6295
MscI TGGCCA 2 cut(s) 3350, 4772
MslI CAYNNNNRTG 2 cut(s) 1750, 5244
Msp20I TGGCCA 2 cut(s) 3350, 4772
MspA1I CMGCKG 4 cut(s) 208, 1492, 2213, 5945
MspI CCGG 6 cut(s) 870, 2200, 2360, 5751, 6065, 6354
MunI CAATTG 5 cut(s) 1974, 2418, 2781, 4008, 5967
Mva1269I GAATGC 9 cut(s) 444, 1054, 2995, 3934, 4084, 4258, 5043, 5149, 5841
MvaI CCWGG 8 cut(s) 538, 2062, 2394, 2575, 2958, 3562, 3615, 5591
NaeI GCCGGC 1 cut(s) 6355
NciI CCSGG 3 cut(s) 2201, 2360, 6066
NdeI CATATG 1 cut(s) 1090
NgoMIV GCCGGC 1 cut(s) 6353
NheI GCTAGC 1 cut(s) 3223
NlaIV GGNNCC 8 cut(s) 102, 535, 868, 1685, 1929, 4215, 4491, 4563
NmeAIII GCCGAG 2 cut(s) 4171, 6129
NmuCI GTSAC 7 cut(s) 2744, 3083, 4234, 4785, 4825, 4957, 6393
NsiI ATGCAT 2 cut(s) 200, 446
NspI RCATGY 6 cut(s) 789, 1031, 1719, 2292, 3266, 5940
PaeI GCATGC 1 cut(s) 1031
PaeR7I CTCGAG 1 cut(s) 2220
PagI TCATGA 1 cut(s) 2524
PaqCI CACCTGC 2 cut(s) 893, 1144
PasI CCCWGGG 1 cut(s) 2957
PceI AGGCCT 2 cut(s) 2156, 4423
PciI ACATGT 1 cut(s) 2288
PciSI GCTCTTC 4 cut(s) 1431, 3859, 5154, 5478
PctI GAATGC 9 cut(s) 444, 1054, 2995, 3934, 4084, 4258, 5043, 5149, 5841
PdiI GCCGGC 1 cut(s) 6355
PdmI GAANNNNTTC 4 cut(s) 1485, 3868, 4809, 6295
PflFI GACNNNGTC 1 cut(s) 5872
PflMI CCANNNNNTGG 2 cut(s) 458, 4619
PfoI TCCNGGA 3 cut(s) 2060, 3560, 3613
PleI GAGTC 8 cut(s) 2011, 2395, 3031, 3373, 3640, 3806, 4822, 6243
PmaCI CACGTG 1 cut(s) 1949
PmlI CACGTG 1 cut(s) 1949
PpsI GAGTC 8 cut(s) 2011, 2395, 3031, 3373, 3640, 3806, 4822, 6243
Ppu21I YACGTR 2 cut(s) 1712, 1949
PscI ACATGT 1 cut(s) 2288
Psp124BI GAGCTC 2 cut(s) 1426, 2003
Psp1406I AACGTT 2 cut(s) 4662, 5834
Psp6I CCWGG 8 cut(s) 536, 2060, 2392, 2573, 2956, 3560, 3613, 5589
PspCI CACGTG 1 cut(s) 1949
PspEI GGTNACC 2 cut(s) 838, 6310
PspFI CCCAGC 3 cut(s) 2910, 3119, 6340
PspGI CCWGG 8 cut(s) 536, 2060, 2392, 2573, 2956, 3560, 3613, 5589
PspN4I GGNNCC 8 cut(s) 102, 535, 868, 1685, 1929, 4215, 4491, 4563
PstI CTGCAG 3 cut(s) 904, 4201, 5977
PstNI CAGNNNCTG 8 cut(s) 899, 1223, 1633, 1646, 2342, 2393, 5573, 5828
PsyI GACNNNGTC 1 cut(s) 5872
PvuII CAGCTG 4 cut(s) 208, 1492, 2213, 5945
RseI CAYNNNNRTG 2 cut(s) 1750, 5244
SacI GAGCTC 2 cut(s) 1426, 2003
SapI GCTCTTC 4 cut(s) 1431, 3859, 5154, 5478
ScaI AGTACT 2 cut(s) 3288, 5277
SchI GAGTC 8 cut(s) 2011, 2395, 3032, 3373, 3640, 3806, 4822, 6244
SfcI CTRYAG 4 cut(s) 900, 3762, 4197, 5973
Sfr274I CTCGAG 1 cut(s) 2220
SinI GGWCC 7 cut(s) 471, 1927, 4562, 4622, 4844, 6191, 6225
SlaI CTCGAG 1 cut(s) 2220
SmiMI CAYNNNNRTG 2 cut(s) 1750, 5244
SpeI ACTAGT 1 cut(s) 5467
SphI GCATGC 1 cut(s) 1031
SseBI AGGCCT 2 cut(s) 2156, 4423
SsiI CCGC 4 cut(s) 449, 2171, 3414, 6387
SspI AATATT 7 cut(s) 561, 1072, 1995, 2052, 2178, 2706, 4533
SstI GAGCTC 2 cut(s) 1426, 2003
StuI AGGCCT 2 cut(s) 2156, 4423
StyI CCWWGG 5 cut(s) 1105, 1656, 4971, 5307, 5898
TaiI ACGT 7 cut(s) 771, 1714, 1951, 2164, 3856, 4665, 5837
TaqI TCGA 8 cut(s) 346, 1722, 2186, 2221, 3671, 3943, 5434, 5730
TaqII GACCGA 2 cut(s) 4253, 6208
TatI WGTACW 8 cut(s) 229, 1327, 3212, 3286, 4759, 5275, 5544, 6360
TauI GCSGC 1 cut(s) 2174
TseFI GTSAC 7 cut(s) 2744, 3083, 4234, 4785, 4825, 4957, 6393
Tsp45I GTSAC 7 cut(s) 2744, 3083, 4234, 4785, 4825, 4957, 6393
TspGWI ACGGA 5 cut(s) 1794, 2226, 3657, 4259, 6412
Tth111I GACNNNGTC 1 cut(s) 5872
Van91I CCANNNNNTGG 2 cut(s) 458, 4619
VneI GTGCAC 3 cut(s) 1464, 4641, 5463
VpaK11BI GGWCC 7 cut(s) 471, 1927, 4562, 4622, 4844, 6191, 6225
XagI CCTNNNNNAGG 4 cut(s) 2150, 4344, 4484, 6243
XapI RAATTY 8 cut(s) 1010, 1143, 1321, 2627, 3397, 5063, 6044, 6400
XbaI TCTAGA 1 cut(s) 2509
XceI RCATGY 6 cut(s) 789, 1031, 1719, 2292, 3266, 5940
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 2220
XmiI GTMKAC 3 cut(s) 463, 3778, 4873
XmnI GAANNNNTTC 4 cut(s) 1485, 3868, 4809, 6295
ZrmI AGTACT 2 cut(s) 3288, 5277
Zsp2I ATGCAT 2 cut(s) 200, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.