RchiOBHm_Chr2g0115531

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007148
Physical Location & Seq
Reverse (-)
27657967 .. 27668076
10110 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48869

Sequence Viewer

Length: 6426 bp
ATGGCAGCACCCTTGCCACTAATGATCTGTATAGAAAGAAATGGCGATGCAAAAGTTCAAGATTCAGAGCCTCCAACACCACACTCTCTCATAAAAATGGGATCAAGAGAGCGTAGTAACATGGAGGATCCAGATGGGACATTGGCAAGTGTTGCTCAATGCATTGAGCAGCTGCGTCAGAGTTCCTCATCTGTACAAGAGAAGGAGTATTCCTTGAAACAGTTGCTGGAGCTTATTGGAACTCGTGAAAATGCTTTCAGTGCTGTTGGATCTCACTCTCAAGCAGTTCCTGTACTAGTTTCTCTTCTCCGATCAGGATCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTCTCTGTGTAAGGAGAATGAACTAAGGGTGAAAGTCTTGCTTGGGGGATGCATTCCACCATTGCTTGGTCTACTCAGGTCCACCTCAGCAGATGGCCAAATTGCAGCAGCAAAGACCATCTATGCTGTTTCTCAAGGTGGTGCAAGGGATCATGTTGGTTCGAAAATTTTTTCAACTGAAGGAGTTGTGCCAGTGCTTTGGGGGCTGCTACAGAAAGGGATCAAGACTGGTAGCTTGGTTGATAGCTTGTTGACCGGAGCATTAAAGAATCTAGCCACCAGCACTGAGGGTTTCTGGGCTGCAACATTACAAGCTGGAGGAGTGGATATACTAGTGAAGTTGCTCACAACTGGACAGCCAAACACTCAAGCTAACGTGTGCTTCCTTCTTGGATGTATGATGATGGAAGACGCATCTGTTTGCTCTAAGGTGCTGGCTGCAGAGGCTACAAAGCAACTTCTCAAGCTTTTAGGTTCTGGCAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTCTTAAATCTCTTTCTGCCCAGTGCAAAGAAGCAAGGCGGGAGATAGCTAACTTCAATGGTATTCCTGTGTTGATAAATGCTACAATAGCCCCTTCAAAAGAATTCATGCAGGGTGAGTATGCCCAGGCATTGCAGGAAAATGCCATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTGATCTCAAGTCTTGGTCAAAGCCTTGAATCATGCACTTCACCTGCCCAAATTGCTGATACATTAGGGGCTTTGGCTTCAGCTCTTATGATATATGATAGCAAAGCAGAATCTACCAGAGCATCGGATCCTGTTGTTATTGAGCAGACTTTGGTTTCACAATTCAAGCCTAGCTTACCATTTCTCGTTCAGGAGCGAACCATAGAGGCCCTTGCAAGTTTGTATGGGAATACTGTACTCTCAGTTAAACTCAATAGTTCAGAAGCAAAACGATTGCTTGTAGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTTATGAGAGCACTTCTTGCTCTTTGTAATAGTGAAGGAAGTCTATGGCGTGCCCTTCAAGGTCGCGAAGGGGTTCAGCTGTTGATATCACTTCTCGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTTCTTTCTAATGAGAATGATGAGAGTAAGTGGGCTATTACTGCTGCTGGTGGCATACCTCCTCTTGTTCAAATTCTAGAGACGGGATCTGCAAAAGCCAAGGAAGATTCAGCATCAATCCTTCGGAACCTCTGCAATCACAGCGAAGATATACGCGCATGTGTTGAAAGTGCTGATGCAGTTCCTGCACTATTGTGGCTTCTGAAGAATGGAAGCCCAAATGGAAAAGAAATTGCAGCAAAGACTTTAAACCATTTAATCCATAAATCTGACACAGCAACCATCAGCCAACTCACTGCATTATTGACCAGTGAACTACCTGAATCTAAGGTATATGTTTTGGATGCTTTAAAAAGTATGCTGTCTGTGGTCCCTCTCAGTGATATATCGCGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATTAAAATATTAAGCTCAAATAAAGAAGAGACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATTTTTGAAGCTAGGAAGGACTTGCGGGAAAGTAGTATTGCTGTTAAAACTCTTTGGTCAGCTGTCAAGTTGCTTAATGTTGAGTCAGGTAATATCCTTGTAGAGGCTTCACGTTGCCTGGCAGCAATATTTCTTTCCATTAAAGAGAACCGGGATGTGGCTGCTGTTGCTAGAGATGTATTATCTCCCTTAGTTGTGCTCGCTAACTCTTCGGTTCTGGAAGTGGCAGAGCTAGCAACATGTGCTTTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAAACAGCAGTGGCTGAAGACATTATTCTCCCAGCTACTAGAGTTTTGCTTGAAGGTACAGTATCTGGAAAGACCCATGCAGCAGCAGCAATTGCTCGCCTGCTCCACTCTCGCCAAATTGACCATGCCTTAACTGATTGTGTGAATCGTGCCGGAACAGTTCTTGCATTAGTTTCTTTTCTGGAATCTGCTAATCATGGATCTGTTGCCACATCAGGGGCACTAGATGCACTTGCTATTCTGTCCAGGTCAGAACGAGCAAGTGGAGAGAAAAAACCTGCCTGGGCAGTTTTAGCTGAATTCCCGAAAAGCATAATCCCTATAGTTTTATCCATTGCTGATGCAACACCCTTGTTGCAGGATAAAGCTATTGAAATATTGTCAAGACTTTGCAGAGATCAACCTGTTGTACTGGGAGACACTGTTGCTAATGCCTCTGGATGTATACCATCAATTGCCAAAAGGGTGATCAATTCATCAAACTCAAAGGTCAAAATTGGAGGTGCTGCACTCCTTATTTGTGCTGCGAAAGTTAGTCATCAGAGAGTTCTAGAAGATCTTAGTGAATCGAACTTATGTACCCATCTCATTCAATCTCTCGTTGCGATGCTTAATTTTTCGGGAAATATAGGGGGTGGTGAGAAGGACTCTATCAGTATTGATATACATATGAAAGAAGAATTAAAGGATGATGGATCTTCTTTAAGTACAGGAGTTATTGATGGTGTGAATTTAGCTGTATGGCTACTCTCTGTTCTTGCTTGTCATGATGATAAATGCAAAATTGCCATTATGGAGGCTGGTGCTGTGGAAGTCCTCACTGACAGGATCTCATATTGTTTTTCCAATTACTCTCAGATTGATTTTAAAGAAGATAGCAGCATATGGATCTGCACTATGCTGCTAGCGATCTTGTTTCAAGACAGAGATATCATACGAGCACATGCAACCATGAAATCCATACCTGTACTTGCCAATTGGTTGAAGTCAGAGGAGTTAGTCGACAGATATTTTGCTGGACAAGCAATGGCCAGCCTAGTCTGTAATGGTAGCAGGGGGACTCTTTTATCTGTTGCAAATTCTGGAGCAGCGGGTGGACTTATCTCACTACTTGGATGTGCTGATGTTGATATTAGTGACCTATTGGATTTGTCAGAAGAATTTGGTTTAGTGCGTTACCCTGAGCAAGTTGCTCTAGAGAGGCTGTTCAGGGTTGAAGATATAAGGGTTGGTGCTACTTCTAGGAAAGCAATACCTGCACTCGTTGATCTTCTCAAACCAATCCCGGATCGTCCTGGAGCACCATTTTTGGCACTTGGGTTTCTTACTCAGCTCGCAAAGGATTGCCCAGCAAATAAAATAGTTATGGTAGAATCAGGAGCTCTGGAAGCGCTGACCAAGTATCTTTCACTTGGCCCACAAGATGCAACAGAAGAAGCTGCTACAGATCTGTTGGGTCTACTATTTGGTAGTGCTGAAATAAGGAAACACGAATCTGCATTTGGTGCTGTCAATCAACTTGTGGCAGTTTTACGGTTAGGTGGAAGAGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTATTCTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAATCTGTCCAACCCTTGGTGGAGATTCTTAATACTGGTGCTGAGAAGGAGCAGCATGCTGCGATTGCTGCATTGGTTAGGCTGCTTAGTGAAAATCCATCAAGAGCCTTAGCAGTTGCAGACGTTGAAATGAATGCAGTGGATGTTCTTTGCAGGATCCTTTCATCAAATTGTTCGATGGAACTGAAAGGAGATGCTGCTGAGTTGTGTTGTGTTCTCTTTGGAAATACAAGAATCAGGTCAACAATGGCTGCAGCTCGATGTGTTGAGCCTCTGGTGTCTCTTCTTGTTACCGAGTTCAGCCCTGCACAACATTCAGTTGTCCGTGCCTTGGATAAACTTGTTGACGATGAACAACTGGGCGAACTCGTTGCTGCTCATGGTGCAGTTATTCCTCTTGTTGGTCTTCTCTATGGTAAGAATTACTTGCTTCATGAGGCTATTTCCAGAGCTCTGGTGAAATTAGGGAAAGACAGACCTGCTTGTAAATCGGAGATGGTGAAAGCCGGTGTTATTGAGAGCATACTTGACATCCTCCATGAAGCACCCGATTTTCTCTGTGCTGCTTTTGCAGAATTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGCCCTTCTGCTTCAAAAGTGGTTGAGCCGCTTTTCCAGCTTCTAACAAGACCAGAATTTGGACCTGATGGGCAGCACAGTTCATTACAGGTTCTTGTCAATATTTTGGAGCATCCACAGTGTCGTTCTGATTATAGGTTGACATCCCACCAGGCTATTGAACCACTTATTCCCCTACTTGATTCTCCGGCTCCTGCAGTACAACAATTGGCAGCTGAGCTTCTATCACATTTACTCTTTGAAGAACATCTTCAAAAGGATACAGTGATACAGCAAGTGATTGGTCCTCTCATACGGGTTCTTGGCTCTGGTATACATATATTGCAGCAGAGAGCTGTGAAAGCGCTTGTCAGTATTGCGCTAGCATGGCCGAATGAAATTGCAAAAGAAGGTGGTGTCACTGAACTGTCCAAGGTGATATTGCTGTCAGATCCATCTCTTCCAAATGCCCTGTGGGAATCAGCTGCTTCTGTTCTATCAAGCATTCTGCAATTCAGTTCTGAATTTTATTTAGAAGTGCCTGTAGCTGTGTTGGTACGGTTGCTTCGCTCTGGATCAGAAGGCACCGTAGTTGGTGCATTGAATGCTCTTTTGGTACTGGAAACTGATGATGCTACCAGCGCAGAAGCAATGGCTGAAAGTGGTGCCATAGAGGCTCTTTTGGACCTTCTAAGATCTCATCAGTGTGAAGAAACAGCTGCAAGGCTGTTGGAAGTATTGTTGAATAATGTAAAGATCAGAGAAACTAAGGCTACCAAGTCTGCCATATTACCATTGTCTCAGTACCTCTTGGATCCACAAACCCAAGCTCAGCAGGCAAGGTTACTAGCAACTCTAGCTCTTGGTGATTTATTCCAGAATGAGGGGCTTGCCCGTAGTACTGACGCTGTCTCAGCTTGTCGTGCTCTGGTAAATGTGCTTGAAGAGCAACCTACTGAAGAAATGAAAGTTGTAGCAATATGTGCTTTGCAAAACCTTGTGATGTACAGTCGGTCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGTGTTCAGGTTGTGTTGGATCTGATTGGTTCAAGTGATCCAGATACGTCCATTCAGGCTGCAATGTTTGTTAAACTTCTCTTTTCAAATCACACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAGGGCGATAACCGCTGCTATTGAAAAGGATTTATGGGCAACTGGAACTGTGAACGATGAGTATCTGAAGGCTCTGAATTCTCTTTTTAGCAATTTCCCACGTTTGAGAGCCACAGAGCCTGCAACTTTAAGCATTCCTCATCTAGTAACATCCCTCAAGACAGGGTCCGAGGCAACTCAAGAAGCTGCGTTGGATGCACTGTTTCTTCTAAGGCAAGCTTGGTCAGCATGCCCAGCTGAAGTTTCTAGGGCTCAGTCAATTGCTGCTGCTGATGCGATCCCTTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGATTTCAAGAGAAAACAGAATTTTTATTACAATGTTTGCCGGGGACATTGGTGGTCATAATCAAGCGTGGGAATAATATGAAGCAGTCAGTGGGAAACCCCAGTGTATTTTGTAAACTTACACTTGGTAACACTCCACCTAGGCAAACCCAGGTTGTTTCAACTGGCCCTAATCCTGAATGGGATGAGAGCTTTTCTTGGTCCTTTGAGAGTCCCCCTAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAGATGGGAAAGAGTTCATTTGGAAAGGTAACTATCCAGATCGATCGAGTAGTAATGCTGGGAGCAGTTGCTGGGGAGTACACCCTATTGCCAGAAAGCAAAAGCGGACCCTCCCGGAATTTAGAAATAGAATTCCAGTGGTCTAACAAAGTAACAAGTAACGACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2141

Amino Acids

228.3

Weight (kDa)

5.32

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 48 - 301 5.4e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 415 - 591 2e-09 ARMC5
C2 PF00168 2014 - 2108 7e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 857, 1108
Acc36I ACCTGC 5 cut(s) 857, 1108, 2578, 3556, 4405
AccB1I GGYRCC 2 cut(s) 5096, 5177
AccB7I CCANNNNNTGG 3 cut(s) 422, 3934, 4592
AccI GTMKAC 5 cut(s) 427, 2737, 3294, 3751, 4846
AccII CGCG 3 cut(s) 1443, 1677, 1912
AciI CCGC 6 cut(s) 910, 2038, 3383, 4562, 5694, 6360
AcoI YGGCCR 3 cut(s) 451, 3321, 4901
AcuI CTGAAG 7 cut(s) 555, 1119, 1745, 2328, 5490, 5768, 5940
AfeI AGCGCT 2 cut(s) 3684, 4878
AflIII ACRYGT 2 cut(s) 732, 2252
AhdI GACNNNNNGTC 1 cut(s) 3923
AhlI ACTAGT 2 cut(s) 295, 688
AjnI CCWGG 7 cut(s) 996, 2130, 2537, 2573, 3586, 4683, 6147
AjuI GAANNNNNNNTTGG 4 cut(s) 3777, 3809, 5108, 5140
AleI CACNNNNGTG 1 cut(s) 1714
Alw21I GWGCWC 9 cut(s) 874, 1390, 2214, 3235, 3595, 3676, 3916, 4372, 5440
Alw26I GTCTC 8 cut(s) 1595, 1937, 1973, 2703, 4203, 5316, 5428, 5669
AlwNI CAGNNNCTG 9 cut(s) 226, 290, 863, 1706, 2306, 2357, 5546, 5801, 6326
Ama87I CYCGRG 1 cut(s) 1472
Aor51HI AGCGCT 2 cut(s) 3684, 4878
AoxI GGCC 9 cut(s) 451, 1263, 3321, 3706, 4534, 4901, 5989, 6163, 6220
ApaI GGGCCC 1 cut(s) 4538
ArsI GACNNNNNNTTYG 2 cut(s) 3287, 3319
Asp700I GAANNNNTTC 4 cut(s) 1449, 3841, 4782, 6268
AspA2I CCTAGG 1 cut(s) 6137
AspLEI GCGC 5 cut(s) 1679, 3685, 4879, 4894, 5156
AsuC2I CCSGG 4 cut(s) 2165, 3578, 6039, 6370
AsuHPI GGTGA 9 cut(s) 397, 998, 1089, 2770, 2942, 4387, 4429, 4960, 5390
AsuII TTCGAA 1 cut(s) 518
AsuNHI GCTAGC 3 cut(s) 2245, 3196, 4894
AvaI CYCGRG 1 cut(s) 1472
AvaII GGWCC 8 cut(s) 435, 1891, 4595, 4817, 5197, 5847, 6198, 6362
AvrII CCTAGG 1 cut(s) 6137
BaeGI GKGCMC 3 cut(s) 1432, 2515, 4538
BaeI ACNNNNGTAYC 2 cut(s) 2854, 2887
BalI TGGCCA 2 cut(s) 453, 3323
BamHI GGATCC 4 cut(s) 127, 1183, 4074, 5326
BanI GGYRCC 2 cut(s) 5096, 5177
BanII GRGCYC 4 cut(s) 3676, 4372, 4538, 5935
BarI GAAGNNNNNNTAC 2 cut(s) 5061, 5093
BauI CACGAG 1 cut(s) 243
BbsI GAAGAC 3 cut(s) 771, 2316, 4316
Bbv12I GWGCWC 9 cut(s) 874, 1390, 2214, 3235, 3595, 3676, 3916, 4372, 5440
BbvCI CCTCAGC 2 cut(s) 442, 5547
BcgI CGANNNNNNTGC 4 cut(s) 4271, 4305, 5947, 5981
BciT130I CCWGG 7 cut(s) 998, 2132, 2539, 2575, 3588, 4685, 6149
BciVI GTATCC 1 cut(s) 4786
BclI TGATCA 2 cut(s) 1344, 2760
BcnI CCSGG 4 cut(s) 2165, 3578, 6039, 6370
BcoDI GTCTC 8 cut(s) 1595, 1937, 1973, 2703, 4203, 5316, 5428, 5669
BcuI ACTAGT 2 cut(s) 295, 688
BfmI CTRYAG 8 cut(s) 566, 795, 864, 2613, 3735, 4170, 4728, 5055
BfoI RGCGCY 2 cut(s) 3686, 4880
BfuAI ACCTGC 5 cut(s) 857, 1108, 2578, 3556, 4405
BfuI GTATCC 1 cut(s) 4786
BglI GCCNNNNNGGC 1 cut(s) 5186
BglII AGATCT 3 cut(s) 2848, 3739, 5207
BlnI CCTAGG 1 cut(s) 6137
BlpI GCTNAGC 2 cut(s) 4749, 5343
BmcAI AGTACT 1 cut(s) 5413
Bme18I GGWCC 8 cut(s) 435, 1891, 4595, 4817, 5197, 5847, 6198, 6362
BmeRI GACNNNNNGTC 1 cut(s) 3923
BmeT110I CYCGRG 1 cut(s) 1472
BmrI ACTGGG 4 cut(s) 886, 2714, 4286, 6093
BmtI GCTAGC 3 cut(s) 2249, 3200, 4898
BmuI ACTGGG 4 cut(s) 886, 2714, 4286, 6093
BpiI GAAGAC 3 cut(s) 771, 2316, 4316
BplI GAGNNNNNCTC 2 cut(s) 2924, 2956
BpmI CTGGAG 4 cut(s) 248, 693, 3396, 3609
Bpu10I CCTNAGC 5 cut(s) 442, 3474, 4027, 4530, 5547
Bpu1102I GCTNAGC 2 cut(s) 4749, 5343
Bpu14I TTCGAA 1 cut(s) 518
BpuEI CTTGAG 8 cut(s) 264, 474, 708, 803, 1048, 1968, 5822, 5844
BpuMI CCSGG 4 cut(s) 2165, 3578, 6039, 6370
Bsa29I ATCGAT 1 cut(s) 6297
BsaBI GATNNNNATC 2 cut(s) 316, 5742
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 2700, 2730
Bse118I RCCGGY 1 cut(s) 4424
Bse3DI GCAATG 8 cut(s) 416, 844, 1001, 2625, 3324, 4524, 5169, 5625
Bse8I GATNNNNATC 2 cut(s) 316, 5742
BseBI CCWGG 7 cut(s) 998, 2132, 2539, 2575, 3588, 4685, 6149
BseCI ATCGAT 1 cut(s) 6297
BseJI GATNNNNATC 2 cut(s) 316, 5742
BseMI GCAATG 8 cut(s) 416, 844, 1001, 2625, 3324, 4524, 5169, 5625
BseRI GAGGAG 3 cut(s) 690, 1572, 3299
BseSI GKGCMC 3 cut(s) 1432, 2515, 4538
BseYI CCCAGC 5 cut(s) 2323, 3640, 5914, 6313, 6326
BsgI GTGCAG 7 cut(s) 1524, 1692, 2784, 3169, 3534, 4209, 4323
Bsh1236I CGCG 3 cut(s) 1443, 1677, 1912
Bsh1285I CGRYCG 1 cut(s) 6301
BshFI GGCC 9 cut(s) 453, 1265, 3323, 3708, 4536, 4903, 5991, 6165, 6222
BshNI GGYRCC 2 cut(s) 5096, 5177
BshVI ATCGAT 1 cut(s) 6297
BsiEI CGRYCG 1 cut(s) 6301
BsiHKAI GWGCWC 9 cut(s) 874, 1390, 2214, 3235, 3595, 3676, 3916, 4372, 5440
BsiHKCI CYCGRG 1 cut(s) 1472
BsiSI CCGG 8 cut(s) 612, 2164, 2445, 3578, 4425, 4721, 6038, 6370
BslFI GGGAC 5 cut(s) 151, 1877, 3364, 6055, 6195
BsmAI GTCTC 8 cut(s) 1595, 1937, 1973, 2703, 4203, 5316, 5428, 5669
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 5 cut(s) 151, 1877, 3364, 6055, 6195
BsmI GAATGC 6 cut(s) 408, 3907, 4057, 5016, 5122, 5814
BsnI GGCC 9 cut(s) 453, 1265, 3323, 3708, 4536, 4903, 5991, 6165, 6222
BsoBI CYCGRG 1 cut(s) 1472
Bsp119I TTCGAA 1 cut(s) 518
Bsp120I GGGCCC 1 cut(s) 4534
Bsp1407I TGTACA 2 cut(s) 193, 5517
Bsp1720I GCTNAGC 2 cut(s) 4749, 5343
Bsp68I TCGCGA 1 cut(s) 1443
BspACI CCGC 6 cut(s) 910, 2038, 3383, 4562, 5694, 6360
BspANI GGCC 9 cut(s) 453, 1265, 3323, 3708, 4536, 4903, 5991, 6165, 6222
BspDI ATCGAT 1 cut(s) 6297
BspFNI CGCG 3 cut(s) 1443, 1677, 1912
BspHI TCATGA 2 cut(s) 3058, 4351
BspMAI CTGCAG 4 cut(s) 799, 868, 4174, 4732
BspMI ACCTGC 5 cut(s) 857, 1108, 2578, 3556, 4405
BspOI GCTAGC 3 cut(s) 2249, 3200, 4898
BspQI GCTCTTC 2 cut(s) 3832, 5451
BspT104I TTCGAA 1 cut(s) 518
BspT107I GGYRCC 2 cut(s) 5096, 5177
BsrDI GCAATG 8 cut(s) 416, 844, 1001, 2625, 3324, 4524, 5169, 5625
BsrFI RCCGGY 1 cut(s) 4424
BsrGI TGTACA 2 cut(s) 193, 5517
BssAI RCCGGY 1 cut(s) 4424
BssNAI GTATAC 2 cut(s) 2738, 4847
BssSI CACGAG 1 cut(s) 243
BssT1I CCWWGG 5 cut(s) 1620, 3933, 4248, 4944, 6137
Bst1107I GTATAC 2 cut(s) 2738, 4847
Bst2BI CACGAG 1 cut(s) 243
Bst2UI CCWGG 7 cut(s) 998, 2132, 2539, 2575, 3588, 4685, 6149
Bst6I CTCTTC 7 cut(s) 309, 1971, 2227, 3832, 4206, 4977, 5451
BstAUI TGTACA 2 cut(s) 193, 5517
BstBI TTCGAA 1 cut(s) 518
BstENI CCTNNNNNAGG 1 cut(s) 2114
BstFNI CGCG 3 cut(s) 1443, 1677, 1912
BstH2I RGCGCY 2 cut(s) 3686, 4880
BstHHI GCGC 5 cut(s) 1679, 3685, 4879, 4894, 5156
BstMAI GTCTC 8 cut(s) 1595, 1937, 1973, 2703, 4203, 5316, 5428, 5669
BstMCI CGRYCG 1 cut(s) 6301
BstNI CCWGG 7 cut(s) 998, 2132, 2539, 2575, 3588, 4685, 6149
BstNSI RCATGY 5 cut(s) 1683, 2256, 3239, 3977, 5913
BstSFI CTRYAG 8 cut(s) 566, 795, 864, 2613, 3735, 4170, 4728, 5055
BstSLI GKGCMC 3 cut(s) 1432, 2515, 4538
BstUI CGCG 3 cut(s) 1443, 1677, 1912
BstV2I GAAGAC 3 cut(s) 771, 2316, 4316
BstXI CCANNNNNNTGG 2 cut(s) 555, 4372
BstZ17I GTATAC 2 cut(s) 2738, 4847
Bsu15I ATCGAT 1 cut(s) 6297
BsuI GTATCC 1 cut(s) 4786
BsuRI GGCC 9 cut(s) 453, 1265, 3323, 3708, 4536, 4903, 5991, 6165, 6222
BsuTUI ATCGAT 1 cut(s) 6297
BtgZI GCGATG 2 cut(s) 60, 2912
BtsI GCAGTG 4 cut(s) 1511, 1815, 2307, 4062
BtuMI TCGCGA 1 cut(s) 1443
BveI ACCTGC 5 cut(s) 857, 1108, 2578, 3556, 4405
CaiI CAGNNNCTG 9 cut(s) 226, 290, 863, 1706, 2306, 2357, 5546, 5801, 6326
CciI TCATGA 2 cut(s) 3058, 4351
CfoI GCGC 5 cut(s) 1679, 3685, 4879, 4894, 5156
Cfr10I RCCGGY 1 cut(s) 4424
ClaI ATCGAT 1 cut(s) 6297
CseI GACGC 3 cut(s) 164, 776, 5426
CspCI CAANNNNNGTGG 2 cut(s) 6221, 6256
DraI TTTAAA 3 cut(s) 1770, 1872, 3160
DriI GACNNNNNGTC 1 cut(s) 3923
EaeI YGGCCR 3 cut(s) 451, 3321, 4901
Eam1104I CTCTTC 7 cut(s) 309, 1971, 2227, 3832, 4206, 4977, 5451
Eam1105I GACNNNNNGTC 1 cut(s) 3923
EarI CTCTTC 7 cut(s) 309, 1971, 2227, 3832, 4206, 4977, 5451
Ecl136II GAGCTC 2 cut(s) 3674, 4370
Eco130I CCWWGG 5 cut(s) 1620, 3933, 4248, 4944, 6137
Eco24I GRGCYC 4 cut(s) 3676, 4372, 4538, 5935
Eco32I GATATC 2 cut(s) 1464, 3223
Eco47I GGWCC 8 cut(s) 435, 1891, 4595, 4817, 5197, 5847, 6198, 6362
Eco47III AGCGCT 2 cut(s) 3684, 4878
Eco53kI GAGCTC 2 cut(s) 3674, 4370
Eco57I CTGAAG 7 cut(s) 555, 1119, 1745, 2328, 5490, 5768, 5940
Eco88I CYCGRG 1 cut(s) 1472
EcoICRI GAGCTC 2 cut(s) 3674, 4370
EcoNI CCTNNNNNAGG 1 cut(s) 2114
EcoO109I RGGNCCY 3 cut(s) 1264, 4534, 4535
EcoRI GAATTC 4 cut(s) 974, 2591, 5758, 6386
EcoRII CCWGG 7 cut(s) 996, 2130, 2537, 2573, 3586, 4683, 6147
EcoRV GATATC 2 cut(s) 1464, 3223
EcoT14I CCWWGG 5 cut(s) 1620, 3933, 4248, 4944, 6137
EcoT22I ATGCAT 2 cut(s) 164, 410
EcoT38I GRGCYC 4 cut(s) 3676, 4372, 4538, 5935
ErhI CCWWGG 5 cut(s) 1620, 3933, 4248, 4944, 6137
Esp3I CGTCTC 1 cut(s) 1595
FaqI GGGAC 5 cut(s) 151, 1877, 3364, 6055, 6195
FauI CCCGC 3 cut(s) 903, 2031, 3376
FauNDI CATATG 3 cut(s) 1054, 2961, 3176
FbaI TGATCA 2 cut(s) 1344, 2760
FblI GTMKAC 5 cut(s) 427, 2737, 3294, 3751, 4846
FriOI GRGCYC 4 cut(s) 3676, 4372, 4538, 5935
GlaI GCGC 5 cut(s) 1678, 3684, 4878, 4893, 5155
GsaI CCCAGC 5 cut(s) 2327, 3644, 5918, 6317, 6330
GsuI CTGGAG 4 cut(s) 248, 693, 3396, 3609
HaeII RGCGCY 2 cut(s) 3686, 4880
HaeIII GGCC 9 cut(s) 453, 1265, 3323, 3708, 4536, 4903, 5991, 6165, 6222
HapII CCGG 8 cut(s) 612, 2164, 2445, 3578, 4425, 4721, 6038, 6370
HgaI GACGC 3 cut(s) 164, 776, 5426
HhaI GCGC 5 cut(s) 1679, 3685, 4879, 4894, 5156
Hin6I GCGC 5 cut(s) 1677, 3683, 4877, 4892, 5154
HinP1I GCGC 5 cut(s) 1677, 3683, 4877, 4892, 5154
HincII GTYRAC 5 cut(s) 609, 3295, 4161, 4264, 4674
HindII GTYRAC 5 cut(s) 609, 3295, 4161, 4264, 4674
HindIII AAGCTT 4 cut(s) 821, 843, 3874, 5898
HpaII CCGG 8 cut(s) 612, 2164, 2445, 3578, 4425, 4721, 6038, 6370
HphI GGTGA 9 cut(s) 397, 998, 1089, 2770, 2942, 4387, 4429, 4960, 5390
HpyCH4IV ACGT 5 cut(s) 732, 2125, 4041, 5603, 5782
HpySE526I ACGT 5 cut(s) 732, 2125, 4041, 5603, 5782
HspAI GCGC 5 cut(s) 1677, 3683, 4877, 4892, 5154
Ksp22I TGATCA 2 cut(s) 1344, 2760
LguI GCTCTTC 2 cut(s) 3832, 5451
MaeII ACGT 5 cut(s) 732, 2125, 4041, 5603, 5782
MfeI CAATTG 6 cut(s) 1938, 2382, 2745, 3268, 4739, 5940
MlsI TGGCCA 2 cut(s) 453, 3323
MluNI TGGCCA 2 cut(s) 453, 3323
MlyI GAGTC 5 cut(s) 1975, 2105, 2933, 3346, 6217
MmeI TCCRAC 7 cut(s) 98, 247, 304, 3952, 5223, 5553, 5853
Mox20I TGGCCA 2 cut(s) 453, 3323
Mph1103I ATGCAT 2 cut(s) 164, 410
MroXI GAANNNNTTC 4 cut(s) 1449, 3841, 4782, 6268
MscI TGGCCA 2 cut(s) 453, 3323
MslI CAYNNNNRTG 3 cut(s) 95, 1714, 5217
Msp20I TGGCCA 2 cut(s) 453, 3323
MspA1I CMGCKG 9 cut(s) 172, 1456, 2075, 3383, 4748, 4997, 5231, 5696, 5918
MspI CCGG 8 cut(s) 612, 2164, 2445, 3578, 4425, 4721, 6038, 6370
MunI CAATTG 6 cut(s) 1938, 2382, 2745, 3268, 4739, 5940
Mva1269I GAATGC 6 cut(s) 408, 3907, 4057, 5016, 5122, 5814
MvaI CCWGG 7 cut(s) 998, 2132, 2539, 2575, 3588, 4685, 6149
MvnI CGCG 3 cut(s) 1443, 1677, 1912
NciI CCSGG 4 cut(s) 2165, 3578, 6039, 6370
NdeI CATATG 3 cut(s) 1054, 2961, 3176
NheI GCTAGC 3 cut(s) 2245, 3196, 4894
NmuCI GTSAC 2 cut(s) 3428, 4930
NruI TCGCGA 1 cut(s) 1443
NsiI ATGCAT 2 cut(s) 164, 410
NspI RCATGY 5 cut(s) 1683, 2256, 3239, 3977, 5913
NspV TTCGAA 1 cut(s) 518
OliI CACNNNNGTG 1 cut(s) 1714
PaeI GCATGC 2 cut(s) 3977, 5913
PagI TCATGA 2 cut(s) 3058, 4351
PaqCI CACCTGC 2 cut(s) 857, 1108
PciI ACATGT 1 cut(s) 2252
PciSI GCTCTTC 2 cut(s) 3832, 5451
PctI GAATGC 6 cut(s) 408, 3907, 4057, 5016, 5122, 5814
PdmI GAANNNNTTC 4 cut(s) 1449, 3841, 4782, 6268
PflFI GACNNNGTC 2 cut(s) 5420, 5845
PflMI CCANNNNNTGG 3 cut(s) 422, 3934, 4592
PfoI TCCNGGA 3 cut(s) 3576, 3586, 6368
Ple19I CGATCG 1 cut(s) 6301
PleI GAGTC 5 cut(s) 1975, 2104, 2933, 3346, 6216
PpsI GAGTC 5 cut(s) 1975, 2104, 2933, 3346, 6216
PscI ACATGT 1 cut(s) 2252
Psp124BI GAGCTC 2 cut(s) 3676, 4372
Psp6I CCWGG 7 cut(s) 996, 2130, 2537, 2573, 3586, 4683, 6147
PspFI CCCAGC 5 cut(s) 2323, 3640, 5914, 6313, 6326
PspGI CCWGG 7 cut(s) 996, 2130, 2537, 2573, 3586, 4683, 6147
PspOMI GGGCCC 1 cut(s) 4534
PstI CTGCAG 4 cut(s) 799, 868, 4174, 4732
PstNI CAGNNNCTG 9 cut(s) 226, 290, 863, 1706, 2306, 2357, 5546, 5801, 6326
PsyI GACNNNGTC 2 cut(s) 5420, 5845
PvuI CGATCG 1 cut(s) 6301
PvuII CAGCTG 7 cut(s) 172, 1456, 2075, 4748, 4997, 5231, 5918
RruI TCGCGA 1 cut(s) 1443
RseI CAYNNNNRTG 3 cut(s) 95, 1714, 5217
SacI GAGCTC 2 cut(s) 3676, 4372
SalI GTCGAC 1 cut(s) 3293
SapI GCTCTTC 2 cut(s) 3832, 5451
ScaI AGTACT 1 cut(s) 5413
SchI GAGTC 5 cut(s) 1975, 2105, 2933, 3346, 6217
SfcI CTRYAG 8 cut(s) 566, 795, 864, 2613, 3735, 4170, 4728, 5055
SfuI TTCGAA 1 cut(s) 518
SinI GGWCC 8 cut(s) 435, 1891, 4595, 4817, 5197, 5847, 6198, 6362
SmiMI CAYNNNNRTG 3 cut(s) 95, 1714, 5217
SmlI CTYRAG 8 cut(s) 279, 489, 723, 818, 1063, 1983, 5837, 5859
SmoI CTYRAG 8 cut(s) 279, 489, 723, 818, 1063, 1983, 5837, 5859
SpeI ACTAGT 2 cut(s) 295, 688
SphI GCATGC 2 cut(s) 3977, 5913
SsiI CCGC 6 cut(s) 910, 2038, 3383, 4562, 5694, 6360
SspI AATATT 6 cut(s) 1036, 1959, 2142, 2670, 4506, 4636
SstI GAGCTC 2 cut(s) 3676, 4372
StyI CCWWGG 5 cut(s) 1620, 3933, 4248, 4944, 6137
TaiI ACGT 5 cut(s) 735, 2128, 4044, 5606, 5785
TaqI TCGA 9 cut(s) 518, 2861, 3294, 3916, 4094, 4177, 5998, 6297, 6301
TaqII GACCGA 1 cut(s) 5514
TauI GCSGC 1 cut(s) 4564
TseFI GTSAC 2 cut(s) 3428, 4930
Tsp45I GTSAC 2 cut(s) 3428, 4930
TspGWI ACGGA 1 cut(s) 4232
Tth111I GACNNNGTC 2 cut(s) 5420, 5845
Van91I CCANNNNNTGG 3 cut(s) 422, 3934, 4592
VpaK11BI GGWCC 8 cut(s) 435, 1891, 4595, 4817, 5197, 5847, 6198, 6362
XagI CCTNNNNNAGG 1 cut(s) 2114
XbaI TCTAGA 3 cut(s) 1597, 2842, 3487
XceI RCATGY 5 cut(s) 1683, 2256, 3239, 3977, 5913
XcmI CCANNNNNNNNNTGG 1 cut(s) 4983
XmaJI CCTAGG 1 cut(s) 6137
XmiI GTMKAC 5 cut(s) 427, 2737, 3294, 3751, 4846
XmnI GAANNNNTTC 4 cut(s) 1449, 3841, 4782, 6268
ZrmI AGTACT 1 cut(s) 5413
Zsp2I ATGCAT 2 cut(s) 164, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.