Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0007018

Physical Location & Seq

Genomic Coordinates Forward (+)

16387244 .. 16392421

Gene Structure 5178 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g24543.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGCGATGGGTAAGTATAGTAGAGTTGATAATAGGAGGTCGGCGGCGAGTTACTGCTCGACGGTGACCATTGTGGTGTTTGTGGCATTGTGCTTGGTGGGGGTTTGGATGATGACGTCATCGTCGGTGGTGCCGGTGCAGAATGTGGATGTGGCTCAGGAGAATAAGAGTGAGGTGGTGAAGGAGGAACAAGTGAGTGAGACCAGTGAGGGGAATAGCAAGCAGTTTGAGGATAACCCGGGGGATTTACCGGAGGATGCGACGAAAGGAGATAGCAATGAGGGTGGTAATCAGGTGGAAGAGAAGCAGGAGGAGAAGGGTGAGGAGAAGAGTGAAGAGAAGATTGAGGAGAAAACAGAAGATGGGTCGAAAACTGAGACTGAGGATGGAGGAAGTAAGACTGAAGAGGGAGAGTCTAAGGGAAATGATGATTCAAATTCTGAAGATGGAGAGAAGAAAAGTGAGGGTGACAATGAGAAAAAGGACGATTTGGGTGAGGGTGAGGGTGACAATGAAAAGAAATCGGATGATGACAATGAAAAGAAAGCAGAAAACACCGATGAAACAAAGGAGAATACTCAAATTGAGGAGAAAGTGGAAACAACTGACAAGGAGCAAGATAGTGAGAAATCGGAGAATGGGCAGGCTGTGAACCAGAGTTCAACTGAGGTGTTTCCTTCTGTTGCTCAGTCAGAGCTTCTGAATGAGACTACTGTCCAAAATGGGTCCTGGTCAACTCAGTCGGCAGAGTCAAAGAATGAAAAGGAGGCTCAACGTTCTTCTGACCAGCAAACTGGTTACAACTGGAAACTTTGTAACTCAACTGCTGGGCCTGATTTCATCCCATGCCTTGACAATTTGCAAGCCATTAGGAGTCTTCAAAGTACCAAGCATTATGAACATCGCGAGAGGCACTGTCCTGAGGAGCCTCCCACATGCCTTCTTCCCCTTCCAGAAGGATATAAACGCCCAATTGAGTGGCCAACGAGCAGGGAAAAGATATGGTACTATAATGTCCCCCATACCAAACTTGCCGAAATTAAGGGGCATCAGAACTGGGTCAAAGTCACTGGCGAATTCCTTACTTTCCCTGGTGGTGGAACCCAATTCAAGCATGGTGCTCTTCATTATATAGATTGGATACAAGAGTCTGTTCCTGATATTGCCTGGGGAAAACGTTCTCGTGTCATATTAGATGTTGGATGTGGGGTTGCAAGCTTTGGAGGATTTTTATTTGACAGAGATGTCCAAGCAATGTCCTTTGCCCCAAAAGATGAGCATGAAGCTCAAGTCCAGTTTGCACTTGAAAGAGGAATTCCTGCTATATCTGCTGTGATGGGCACACAGAGACTTCCTTATCCTAGTAGAGTTTTTGATGTTGTCCACTGTGCCCGCTGCAGGGTTCCCTGGCACATAGAAGGTGGTAAGCTTCTATTAGAGCTGAATCGTGTGTTGAGACCTGGTGGTTTCTTTGTGTGGTCTGCTACCCCAGTATATCAGAAGAAACATGACGATGTTGAAATATGGGAAGCCATGAAGGAACTAACAGAGAAGATTTGTTGGAAACTTGTCACAATTAATAAGGATGCACTAAATGGAATTGGTGCAGCTATATACAGAAAGCCTACCACCAATGAGTGTTATGAGCAAAGATCACAAAACCACCCTCCTATCTGTGACAAGTCTGATGATCCGAATGCAGCATGGAAAGTACCATTACAAGCATGCTTGCACAAAGTTCCTGTAGATGCATCAGAGCGTGGATCTCAGTGGCCTGAGCAATGGCCAGCAAGGTTGGACAAGGCGCCATATTGGTTGTTGAGTTCTCAGACTGGAGTTTATGGAAAACCGGCTCCTGAGGATTTCACTGCAGACTATGAGCACTGGAAACGCGTGGTAGACAAGTCTTATTTAAATGGAATGGGCATTAATTGGTCATCGGTTAGGAATGTCATGGATATGAGATCTGTTTATGGAGGATTTGCTGCGGCTCTGAAAGATTTGAAACTTTGGGTCATGAATATAGTCACGATAGACTCTCCAGACACCTTACCCATAATTTATGAGCGAGGTCTTTTTGGCATGTATCATGATTGGTGTGAATCATTTAGCACCTATCCTAGGTCTTATGATCTTCTCCACGCAGACCATCTGTTTTCCCTGCTCAAGAAGAGGTGCAAGTTAGTCGCTGTAGTTGCAGAGGTTGATCGGATCCTTCGACCAGAGGGAAAACTTATTGTTCGGGACACTGTCGAGACAATTAATGAGCTGGAGAGCATGTTGAAGTCTATGCAGTGGGAGGTTCGCATGACCTATTCCAAGGACAAGGAGGGATTGCTATGTGTCCAGAAGTCGATGTGGCGGCCTAAAGAGACGGAGACAGTCAAGTACGCCATTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

801

Amino Acids

90.45

Weight (kDa)

5.02

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	279 - 784	1.1e-231	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	398 - 492	6.7e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64030
fragaria_vesca	FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543
malus_domestica	MD15G1134500.v1.1
prunus_persica	Prupe.4G273900_v2.0.a1 Prupe.4G273900_v2.0.a1
pyrus_communis	pycom15g12110 pycom505g00120
rosa_chinensis	RchiOBHm_Chr2g0121981
rosa_laevigata	RLG00000018644
rosa_multiflora	Rmu_sc0003880.1_g000014
rosa_roxburghii	Rroxscaffold_2G00122100
rosa_rugosa	Rorug02G0235700 Rorug02G0235800
rosa_samantha	Rh2AG292400 Rh2BG302900 Rh2CG281500 Rh2DG316600 Rh2DG316700 Rh4AG294200 Rh4BG300200
rosa_wichuraiana	Rw2G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	121, 1244
AatII	GACGTC	1 cut(s)	119
AccB1I	GGYRCC	2 cut(s)	130, 1804
AccI	GTMKAC	1 cut(s)	1899
AccII	CGCG	2 cut(s)	906, 1893
AciI	CCGC	4 cut(s)	44, 1393, 1988, 2362
AclI	AACGTT	2 cut(s)	775, 1177
AclWI	GGATC	4 cut(s)	1685, 1771, 2206, 2219
AcoI	YGGCCR	2 cut(s)	980, 1784
AcsI	RAATTY	3 cut(s)	436, 1076, 1314
AcuI	CTGAAG	2 cut(s)	423, 462
AcyI	GRCGYC	2 cut(s)	116, 1805
AfaI	GTAC	4 cut(s)	886, 1007, 1713, 2390
AfiI	CCNNNNNNNGG	5 cut(s)	210, 1097, 1398, 1399, 2121
AflIII	ACRYGT	1 cut(s)	1891
AgsI	TTSAA	8 cut(s)	435, 663, 881, 1111, 1307, 1520, 2005, 2284
AjnI	CCWGG	5 cut(s)	728, 1090, 1166, 1406, 1459
AluBI	AGCT	7 cut(s)	697, 1218, 1286, 1429, 1441, 1610, 2269
AluI	AGCT	7 cut(s)	697, 1218, 1286, 1429, 1441, 1610, 2269
Alw21I	GWGCWC	2 cut(s)	1123, 1884
Alw26I	GTCTC	8 cut(s)	194, 371, 701, 1342, 1450, 2249, 2366, 2372
AlwI	GGATC	4 cut(s)	1685, 1771, 2206, 2219
Ama87I	CYCGRG	1 cut(s)	238
AoxI	GGCC	5 cut(s)	830, 980, 1772, 1784, 2363
ApeKI	GCWGC	4 cut(s)	1395, 1607, 1700, 1985
ApoI	RAATTY	3 cut(s)	436, 1076, 1314
AseI	ATTAAT	3 cut(s)	1578, 1929, 2262
Asp700I	GAANNNNTTC	1 cut(s)	1177
AspA2I	CCTAGG	1 cut(s)	2120
AspLEI	GCGC	1 cut(s)	1807
AspS9I	GGNCC	2 cut(s)	726, 830
AsuC2I	CCSGG	2 cut(s)	239, 240
AsuHPI	GGTGA	7 cut(s)	76, 190, 332, 479, 506, 512, 518
AvaI	CYCGRG	1 cut(s)	238
AvaII	GGWCC	1 cut(s)	726
AvrII	CCTAGG	1 cut(s)	2120
AxyI	CCTNAGG	2 cut(s)	921, 1857
BaeGI	GKGCMC	2 cut(s)	1343, 1393
BaeI	ACNNNNGTAYC	2 cut(s)	1133, 1166
BalI	TGGCCA	2 cut(s)	982, 1786
BamHI	GGATCC	1 cut(s)	2211
BanI	GGYRCC	2 cut(s)	130, 1804
BauI	CACGAG	1 cut(s)	1182
BbsI	GAAGAC	1 cut(s)	869
Bbv12I	GWGCWC	2 cut(s)	1123, 1884
BbvI	GCAGC	4 cut(s)	1382, 1619, 1712, 1972
BccI	CCATC	5 cut(s)	356, 380, 440, 1330, 2157
BcgI	CGANNNNNNTGC	2 cut(s)	2167, 2201
BciT130I	CCWGG	5 cut(s)	730, 1092, 1168, 1408, 1461
BciVI	GTATCC	1 cut(s)	1134
BcnI	CCSGG	2 cut(s)	239, 240
BcoDI	GTCTC	8 cut(s)	194, 371, 701, 1342, 1450, 2249, 2366, 2372
BfaI	CTAG	2 cut(s)	1362, 2121
BfmI	CTRYAG	4 cut(s)	1396, 1743, 1869, 2190
BfoI	RGCGCY	1 cut(s)	1808
BfuI	GTATCC	1 cut(s)	1134
BglII	AGATCT	1 cut(s)	1964
BisI	GCNGC	7 cut(s)	45, 1396, 1608, 1701, 1986, 1989, 2363
BlnI	CCTAGG	1 cut(s)	2120
BlsI	GCNGC	7 cut(s)	46, 1397, 1609, 1702, 1987, 1990, 2364
Bme1390I	CCNGG	7 cut(s)	239, 240, 730, 1092, 1168, 1408, 1461
Bme18I	GGWCC	1 cut(s)	726
BmeT110I	CYCGRG	1 cut(s)	238
BmgT120I	GGNCC	2 cut(s)	726, 830
BmiI	GGNNCC	8 cut(s)	132, 727, 927, 1102, 1404, 1806, 1854, 2213
BmrFI	CCNGG	7 cut(s)	239, 240, 730, 1092, 1168, 1408, 1461
BmrI	ACTGGG	2 cut(s)	1066, 1484
BmsI	GCATC	5 cut(s)	247, 1057, 1576, 1738, 1760
BmuI	ACTGGG	2 cut(s)	1066, 1484
BoxI	GACNNNNGTC	1 cut(s)	713
BpiI	GAAGAC	1 cut(s)	869
BpmI	CTGGAG	3 cut(s)	1854, 2025, 2291
Bpu10I	CCTNAGC	2 cut(s)	156, 1776
BpuEI	CTTGAG	2 cut(s)	1272, 2150
BpuMI	CCSGG	2 cut(s)	239, 240
BsaHI	GRCGYC	2 cut(s)	116, 1805
BsaI	GGTCTC	2 cut(s)	194, 1450
BsaJI	CCNNGG	7 cut(s)	238, 239, 1090, 1167, 1406, 2120, 2319
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	2264, 2294
Bsc4I	CCNNNNNNNGG	5 cut(s)	210, 1097, 1398, 1399, 2121
Bse118I	RCCGGY	2 cut(s)	133, 1849
Bse1I	ACTGG	9 cut(s)	204, 799, 809, 1061, 1075, 1294, 1490, 1837, 1889
Bse21I	CCTNAGG	2 cut(s)	921, 1857
Bse3DI	GCAATG	4 cut(s)	283, 1260, 1787, 2394
BseBI	CCWGG	5 cut(s)	730, 1092, 1168, 1408, 1461
BseDI	CCNNGG	7 cut(s)	238, 239, 1090, 1167, 1406, 2120, 2319
BseGI	GGATG	8 cut(s)	114, 154, 262, 391, 532, 840, 1208, 1591
BseLI	CCNNNNNNNGG	5 cut(s)	210, 1097, 1398, 1399, 2121
BseMI	GCAATG	4 cut(s)	283, 1260, 1787, 2394
BseNI	ACTGG	9 cut(s)	204, 799, 809, 1061, 1075, 1294, 1490, 1837, 1889
BseRI	GAGGAG	5 cut(s)	326, 338, 362, 602, 938
BseSI	GKGCMC	2 cut(s)	1343, 1393
BseXI	GCAGC	4 cut(s)	1382, 1619, 1712, 1972
BseYI	CCCAGC	1 cut(s)	827
BsgI	GTGCAG	2 cut(s)	158, 1626
Bsh1236I	CGCG	2 cut(s)	906, 1893
BshFI	GGCC	5 cut(s)	832, 982, 1774, 1786, 2365
BshNI	GGYRCC	2 cut(s)	130, 1804
BsiHKAI	GWGCWC	2 cut(s)	1123, 1884
BsiHKCI	CYCGRG	1 cut(s)	238
BsiSI	CCGG	4 cut(s)	134, 239, 251, 1850
BslFI	GGGAC	2 cut(s)	1001, 2258
BslI	CCNNNNNNNGG	5 cut(s)	210, 1097, 1398, 1399, 2121
BsmAI	GTCTC	8 cut(s)	194, 371, 701, 1342, 1450, 2249, 2366, 2372
BsmBI	CGTCTC	1 cut(s)	2366
BsmFI	GGGAC	2 cut(s)	1001, 2258
BsmI	GAATGC	1 cut(s)	1702
BsnI	GGCC	5 cut(s)	832, 982, 1774, 1786, 2365
Bso31I	GGTCTC	2 cut(s)	194, 1450
BsoBI	CYCGRG	1 cut(s)	238
Bsp1286I	GDGCHC	4 cut(s)	1123, 1343, 1393, 1884
Bsp143I	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
Bsp68I	TCGCGA	1 cut(s)	906
BspACI	CCGC	4 cut(s)	44, 1393, 1988, 2362
BspANI	GGCC	5 cut(s)	832, 982, 1774, 1786, 2365
BspFNI	CGCG	2 cut(s)	906, 1893
BspHI	TCATGA	2 cut(s)	2016, 2089
BspLI	GGNNCC	8 cut(s)	132, 727, 927, 1102, 1404, 1806, 1854, 2213
BspMAI	CTGCAG	2 cut(s)	1400, 1873
BspPI	GGATC	4 cut(s)	1685, 1771, 2206, 2219
BspQI	GCTCTTC	1 cut(s)	1128
BspT107I	GGYRCC	2 cut(s)	130, 1804
BspTNI	GGTCTC	2 cut(s)	194, 1450
BsrDI	GCAATG	4 cut(s)	283, 1260, 1787, 2394
BsrFI	RCCGGY	2 cut(s)	133, 1849
BsrI	ACTGG	9 cut(s)	204, 799, 809, 1061, 1075, 1294, 1490, 1837, 1889
BssAI	RCCGGY	2 cut(s)	133, 1849
BssECI	CCNNGG	7 cut(s)	238, 239, 1090, 1167, 1406, 2120, 2319
BssMI	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
BssNI	GRCGYC	2 cut(s)	116, 1805
BssSI	CACGAG	1 cut(s)	1182
BssT1I	CCWWGG	2 cut(s)	2120, 2319
Bst2BI	CACGAG	1 cut(s)	1182
Bst2UI	CCWGG	5 cut(s)	730, 1092, 1168, 1408, 1461
Bst4CI	ACNGT	6 cut(s)	64, 715, 917, 1388, 2251, 2383
Bst6I	CTCTTC	6 cut(s)	294, 323, 330, 399, 1128, 2165
BstACI	GRCGYC	2 cut(s)	116, 1805
BstC8I	GCNNGC	8 cut(s)	221, 645, 864, 1216, 1393, 1726, 1730, 1788
BstEII	GGTNACC	1 cut(s)	64
BstENI	CCTNNNNNAGG	1 cut(s)	2119
BstF5I	GGATG	8 cut(s)	114, 154, 262, 391, 532, 840, 1208, 1591
BstFNI	CGCG	2 cut(s)	906, 1893
BstH2I	RGCGCY	1 cut(s)	1808
BstHHI	GCGC	1 cut(s)	1807
BstKTI	GATC	7 cut(s)	1655, 1693, 1766, 1967, 2134, 2209, 2214
BstMAI	GTCTC	8 cut(s)	194, 371, 701, 1342, 1450, 2249, 2366, 2372
BstMBI	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
BstMWI	GCNNNNNNNGC	2 cut(s)	1328, 2195
BstNI	CCWGG	5 cut(s)	730, 1092, 1168, 1408, 1461
BstNSI	RCATGY	4 cut(s)	939, 1728, 2086, 2281
BstPAI	GACNNNNGTC	1 cut(s)	713
BstPI	GGTNACC	1 cut(s)	64
BstSCI	CCNGG	7 cut(s)	237, 238, 728, 1090, 1166, 1406, 1459
BstSFI	CTRYAG	4 cut(s)	1396, 1743, 1869, 2190
BstSLI	GKGCMC	2 cut(s)	1343, 1393
BstUI	CGCG	2 cut(s)	906, 1893
BstV1I	GCAGC	4 cut(s)	1382, 1619, 1712, 1972
BstV2I	GAAGAC	1 cut(s)	869
BstX2I	RGATCY	3 cut(s)	1763, 1964, 2211
BstXI	CCANNNNNNTGG	2 cut(s)	794, 978
BstYI	RGATCY	3 cut(s)	1763, 1964, 2211
Bsu36I	CCTNAGG	2 cut(s)	921, 1857
BsuI	GTATCC	1 cut(s)	1134
BsuRI	GGCC	5 cut(s)	832, 982, 1774, 1786, 2365
BtgZI	GCGATG	2 cut(s)	20, 887
BtsCI	GGATG	8 cut(s)	114, 154, 262, 391, 532, 840, 1208, 1591
BtsI	GCAGTG	2 cut(s)	1866, 2300
BtsIMutI	CAGTG	9 cut(s)	211, 913, 1068, 1384, 1775, 1866, 1882, 2247, 2300
BtuMI	TCGCGA	1 cut(s)	906
Cac8I	GCNNGC	8 cut(s)	221, 645, 864, 1216, 1393, 1726, 1730, 1788
CciI	TCATGA	2 cut(s)	2016, 2089
CfoI	GCGC	1 cut(s)	1807
Cfr10I	RCCGGY	2 cut(s)	133, 1849
Cfr13I	GGNCC	2 cut(s)	726, 830
Cfr9I	CCCGGG	1 cut(s)	238
CsiI	ACCWGGT	1 cut(s)	1459
Csp6I	GTAC	4 cut(s)	885, 1006, 1712, 2389
CspCI	CAANNNNNGTGG	2 cut(s)	265, 300
CviQI	GTAC	4 cut(s)	885, 1006, 1712, 2389
DinI	GGCGCC	1 cut(s)	1806
DpnI	GATC	7 cut(s)	1654, 1692, 1765, 1966, 2133, 2208, 2213
DpnII	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
DraI	TTTAAA	1 cut(s)	1914
DrdI	GACNNNNNNGTC	2 cut(s)	121, 1244
DseDI	GACNNNNNNGTC	2 cut(s)	121, 1244
EaeI	YGGCCR	2 cut(s)	980, 1784
Eam1104I	CTCTTC	6 cut(s)	294, 323, 330, 399, 1128, 2165
EarI	CTCTTC	6 cut(s)	294, 323, 330, 399, 1128, 2165
Eco130I	CCWWGG	2 cut(s)	2120, 2319
Eco31I	GGTCTC	2 cut(s)	194, 1450
Eco47I	GGWCC	1 cut(s)	726
Eco57I	CTGAAG	2 cut(s)	423, 462
Eco81I	CCTNAGG	2 cut(s)	921, 1857
Eco88I	CYCGRG	1 cut(s)	238
Eco91I	GGTNACC	1 cut(s)	64
EcoNI	CCTNNNNNAGG	1 cut(s)	2119
EcoO109I	RGGNCCY	1 cut(s)	726
EcoO65I	GGTNACC	1 cut(s)	64
EcoRI	GAATTC	2 cut(s)	1076, 1314
EcoRII	CCWGG	5 cut(s)	728, 1090, 1166, 1406, 1459
EcoT14I	CCWWGG	2 cut(s)	2120, 2319
EcoT22I	ATGCAT	1 cut(s)	1753
EgeI	GGCGCC	1 cut(s)	1806
EheI	GGCGCC	1 cut(s)	1806
ErhI	CCWWGG	2 cut(s)	2120, 2319
Esp3I	CGTCTC	1 cut(s)	2366
FaqI	GGGAC	2 cut(s)	1001, 2258
FauI	CCCGC	1 cut(s)	1400
FblI	GTMKAC	1 cut(s)	1899
Fnu4HI	GCNGC	7 cut(s)	45, 1396, 1608, 1701, 1986, 1989, 2363
FokI	GGATG	8 cut(s)	121, 161, 269, 398, 539, 827, 1215, 1598
Fsp4HI	GCNGC	7 cut(s)	45, 1396, 1608, 1701, 1986, 1989, 2363
FspBI	CTAG	2 cut(s)	1362, 2121
GlaI	GCGC	1 cut(s)	1806
GluI	GCNGC	7 cut(s)	45, 1396, 1608, 1701, 1986, 1989, 2363
GsaI	CCCAGC	1 cut(s)	831
GsuI	CTGGAG	3 cut(s)	1854, 2025, 2291
HaeII	RGCGCY	1 cut(s)	1808
HaeIII	GGCC	5 cut(s)	832, 982, 1774, 1786, 2365
HapII	CCGG	4 cut(s)	134, 239, 251, 1850
HhaI	GCGC	1 cut(s)	1807
Hin1I	GRCGYC	2 cut(s)	116, 1805
Hin6I	GCGC	1 cut(s)	1805
HinP1I	GCGC	1 cut(s)	1805
HincII	GTYRAC	1 cut(s)	735
HindII	GTYRAC	1 cut(s)	735
HindIII	AAGCTT	2 cut(s)	1216, 1427
HinfI	GANTC	8 cut(s)	413, 431, 749, 874, 1148, 1444, 2036, 2102
HpaII	CCGG	4 cut(s)	134, 239, 251, 1850
HphI	GGTGA	7 cut(s)	76, 190, 332, 479, 506, 512, 518
Hpy166II	GTNNAC	4 cut(s)	652, 735, 1384, 1900
Hpy8I	GTNNAC	4 cut(s)	652, 735, 1384, 1900
Hpy99I	CGWCG	3 cut(s)	64, 127, 265
HpyAV	CCTTC	9 cut(s)	175, 310, 687, 950, 950, 959, 1412, 1531, 2225
HpyCH4III	ACNGT	6 cut(s)	64, 715, 917, 1388, 2251, 2383
HpyCH4IV	ACGT	3 cut(s)	116, 775, 1177
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1328, 2195
HpySE526I	ACGT	3 cut(s)	116, 775, 1177
Hsp92I	GRCGYC	2 cut(s)	116, 1805
HspAI	GCGC	1 cut(s)	1805
KasI	GGCGCC	1 cut(s)	1804
Kzo9I	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
LguI	GCTCTTC	1 cut(s)	1128
LmnI	GCTCC	3 cut(s)	613, 925, 1858
Lsp1109I	GCAGC	4 cut(s)	1382, 1619, 1712, 1972
LweI	GCATC	5 cut(s)	247, 1057, 1576, 1738, 1760
MabI	ACCWGGT	1 cut(s)	1459
MaeI	CTAG	2 cut(s)	1362, 2121
MaeII	ACGT	3 cut(s)	116, 775, 1177
MalI	GATC	7 cut(s)	1654, 1692, 1765, 1966, 2133, 2208, 2213
MboI	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
MfeI	CAATTG	1 cut(s)	972
MflI	RGATCY	3 cut(s)	1763, 1964, 2211
MhlI	GDGCHC	4 cut(s)	1123, 1343, 1393, 1884
MlsI	TGGCCA	2 cut(s)	982, 1786
MluI	ACGCGT	1 cut(s)	1891
MluNI	TGGCCA	2 cut(s)	982, 1786
Mly113I	GGCGCC	1 cut(s)	1805
MlyI	GAGTC	5 cut(s)	422, 758, 883, 1157, 2030
MmeI	TCCRAC	3 cut(s)	1180, 1541, 1776
Mox20I	TGGCCA	2 cut(s)	982, 1786
Mph1103I	ATGCAT	1 cut(s)	1753
MroXI	GAANNNNTTC	1 cut(s)	1177
MscI	TGGCCA	2 cut(s)	982, 1786
MseI	TTAA	6 cut(s)	1041, 1578, 1913, 1929, 2262, 2401
MslI	CAYNNNNRTG	3 cut(s)	74, 1512, 1958
Msp20I	TGGCCA	2 cut(s)	982, 1786
MspA1I	CMGCKG	1 cut(s)	1395
MspI	CCGG	4 cut(s)	134, 239, 251, 1850
MspR9I	CCNGG	7 cut(s)	239, 240, 730, 1092, 1168, 1408, 1461
MunI	CAATTG	1 cut(s)	972
Mva1269I	GAATGC	1 cut(s)	1702
MvaI	CCWGG	5 cut(s)	730, 1092, 1168, 1408, 1461
MvnI	CGCG	2 cut(s)	906, 1893
MwoI	GCNNNNNNNGC	2 cut(s)	1328, 2195
NarI	GGCGCC	1 cut(s)	1805
NciI	CCSGG	2 cut(s)	239, 240
NdeII	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
NlaIV	GGNNCC	8 cut(s)	132, 727, 927, 1102, 1404, 1806, 1854, 2213
NmuCI	GTSAC	7 cut(s)	64, 467, 506, 1066, 1570, 1676, 2026
NruI	TCGCGA	1 cut(s)	906
NsiI	ATGCAT	1 cut(s)	1753
NspI	RCATGY	4 cut(s)	939, 1728, 2086, 2281
PaeI	GCATGC	1 cut(s)	1728
PagI	TCATGA	2 cut(s)	2016, 2089
PciSI	GCTCTTC	1 cut(s)	1128
PcsI	WCGNNNNNNNCGW	1 cut(s)	2215
PctI	GAATGC	1 cut(s)	1702
PdmI	GAANNNNTTC	1 cut(s)	1177
PfeI	GAWTC	3 cut(s)	431, 1444, 2102
PflFI	GACNNNGTC	1 cut(s)	2249
PkrI	GCNGC	7 cut(s)	46, 1397, 1609, 1702, 1987, 1990, 2364
PleI	GAGTC	5 cut(s)	421, 757, 882, 1156, 2030
PluTI	GGCGCC	1 cut(s)	1808
PpsI	GAGTC	5 cut(s)	421, 757, 882, 1156, 2030
PpuMI	RGGWCCY	1 cut(s)	726
PshAI	GACNNNNGTC	1 cut(s)	713
PshBI	ATTAAT	3 cut(s)	1578, 1929, 2262
Psp1406I	AACGTT	2 cut(s)	775, 1177
Psp5II	RGGWCCY	1 cut(s)	726
Psp6I	CCWGG	5 cut(s)	728, 1090, 1166, 1406, 1459
PspEI	GGTNACC	1 cut(s)	64
PspFI	CCCAGC	1 cut(s)	827
PspGI	CCWGG	5 cut(s)	728, 1090, 1166, 1406, 1459
PspN4I	GGNNCC	8 cut(s)	132, 727, 927, 1102, 1404, 1806, 1854, 2213
PspPI	GGNCC	2 cut(s)	726, 830
PspPPI	RGGWCCY	1 cut(s)	726
PstI	CTGCAG	2 cut(s)	1400, 1873
PsuI	RGATCY	3 cut(s)	1763, 1964, 2211
PsyI	GACNNNGTC	1 cut(s)	2249
RruI	TCGCGA	1 cut(s)	906
RsaI	GTAC	4 cut(s)	886, 1007, 1713, 2390
RsaNI	GTAC	4 cut(s)	885, 1006, 1712, 2389
RseI	CAYNNNNRTG	3 cut(s)	74, 1512, 1958
SapI	GCTCTTC	1 cut(s)	1128
SaqAI	TTAA	6 cut(s)	1041, 1578, 1913, 1929, 2262, 2401
SatI	GCNGC	7 cut(s)	45, 1396, 1608, 1701, 1986, 1989, 2363
Sau3AI	GATC	7 cut(s)	1652, 1690, 1763, 1964, 2131, 2206, 2211
Sau96I	GGNCC	2 cut(s)	726, 830
SchI	GAGTC	5 cut(s)	422, 758, 883, 1157, 2030
ScrFI	CCNGG	7 cut(s)	239, 240, 730, 1092, 1168, 1408, 1461
SduI	GDGCHC	4 cut(s)	1123, 1343, 1393, 1884
SexAI	ACCWGGT	1 cut(s)	1459
SfaNI	GCATC	5 cut(s)	247, 1057, 1576, 1738, 1760
SfcI	CTRYAG	4 cut(s)	1396, 1743, 1869, 2190
SfoI	GGCGCC	1 cut(s)	1806
SinI	GGWCC	1 cut(s)	726
SmaI	CCCGGG	1 cut(s)	240
SmiI	ATTTAAAT	1 cut(s)	1914
SmiMI	CAYNNNNRTG	3 cut(s)	74, 1512, 1958
SmlI	CTYRAG	2 cut(s)	1287, 2165
SmoI	CTYRAG	2 cut(s)	1287, 2165
SphI	GCATGC	1 cut(s)	1728
SsiI	CCGC	4 cut(s)	44, 1393, 1988, 2362
SspDI	GGCGCC	1 cut(s)	1804
SspMI	CTAG	2 cut(s)	1362, 2121
StyD4I	CCNGG	7 cut(s)	237, 238, 728, 1090, 1166, 1406, 1459
StyI	CCWWGG	2 cut(s)	2120, 2319
SwaI	ATTTAAAT	1 cut(s)	1914
TaaI	ACNGT	6 cut(s)	64, 715, 917, 1388, 2251, 2383
TaiI	ACGT	3 cut(s)	119, 778, 1180
TaqI	TCGA	5 cut(s)	59, 368, 2218, 2253, 2354
TauI	GCSGC	3 cut(s)	47, 1991, 2365
TfiI	GAWTC	3 cut(s)	431, 1444, 2102
Tru1I	TTAA	6 cut(s)	1041, 1578, 1913, 1929, 2262, 2401
Tru9I	TTAA	6 cut(s)	1041, 1578, 1913, 1929, 2262, 2401
TscAI	CASTG	9 cut(s)	211, 920, 1075, 1391, 1775, 1873, 1889, 2254, 2300
TseFI	GTSAC	7 cut(s)	64, 467, 506, 1066, 1570, 1676, 2026
TseI	GCWGC	4 cut(s)	1395, 1607, 1700, 1985
Tsp45I	GTSAC	7 cut(s)	64, 467, 506, 1066, 1570, 1676, 2026
TspGWI	ACGGA	1 cut(s)	2390
TspMI	CCCGGG	1 cut(s)	238
TspRI	CASTG	9 cut(s)	211, 920, 1075, 1391, 1775, 1873, 1889, 2254, 2300
Tth111I	GACNNNGTC	1 cut(s)	2249
VpaK11BI	GGWCC	1 cut(s)	726
VspI	ATTAAT	3 cut(s)	1578, 1929, 2262
XagI	CCTNNNNNAGG	1 cut(s)	2119
XapI	RAATTY	3 cut(s)	436, 1076, 1314
XceI	RCATGY	4 cut(s)	939, 1728, 2086, 2281
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1112
XmaI	CCCGGG	1 cut(s)	238
XmaJI	CCTAGG	1 cut(s)	2120
XmiI	GTMKAC	1 cut(s)	1899
XmnI	GAANNNNTTC	1 cut(s)	1177
XspI	CTAG	2 cut(s)	1362, 2121
ZraI	GACGTC	1 cut(s)	117
Zsp2I	ATGCAT	1 cut(s)	1753

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.