Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0007018

Physical Location & Seq

Genomic Coordinates Forward (+)

34186404 .. 34190877

Gene Structure 4474 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGGCGTTAGGGAAGTATAGTAGAGTTGATAATAGGAGGTCGGCGTCGAGTTACTGCTCGACGGTGACCATTGTGGTGTTTGTGGCATTGTGCTTGGTGGGGGTTTGGATGATGACGTCGTCGTCGGTGGTGCCGGTTCAGAATGTAGATGTAGGTCAGGAGAACAAGAGTGAGGTGAAGGAGCAAGTGATTGAAACCAATGAGGGGAATAACAACAGGCAGTTTGAAGATAACCCCGGTGATTTACCGGAGGATGCGACGAAAGGAGATACCAATGAGGGTGCTGCTCAGGTGGAAGAGAAGCCCGAGGTGAAAACTGAAGAGAAGGTTGAGGAGAAAACTGAAGAGAAGGTTCAGGAGAAAACTGAAGAGAAGGTTGAGGAGAAAACAGAAGATGGGTTGAAGACTGAAACGGAAGATGGAGGAAGTAAGACAGAGGAGGGAGAGTCTAAGGTGGAAAATGATGAATCGAATTCGGAAGATGGAGAGAAGAAAAGTGATGGTGACGATGAGAAAAAGGATGATTCGGGCGAGGGTGACAATGAAAAGAAATCGGATGATGACAATGAGAAGAAAACGGAGAATTCTGATGAAACAAAGGATGCGGAGAAGGAGAATATTCAGATTGACGAGAAAGTGGAAAAAACTGATAAGGAGCAAGATAGTGAGAAATCGGATAATGGGCAGACTGTAAACCAGACTTCAAATGAGGTATTTCCTTCTGTTGCTCAGTCAGAGCTTTTGAATGAGACTGCAACGCAAAATGGGTCATGGTCAACTCAGTCGACAGAGTCAAAGAATGAGAAGGAGGCTCAACGTTCTTCTGACCAGCAAATTGATTACAATTGGAAACTTTGTAATTCAACTGCTGGGCCTGATTTCATCCCATGCCTTGACAATTTGCAAGCCATTAGGAGTCTTCACAGTACCAAGCATTATGAACATCGGGAGAGGCACTGTCCGGAGGAGCCTCCCACCTGCCTCCTTCCTCTTCCAGAAGGATATAAACGCCCAATTGAGTGGCCAATGAGCAGGGAAAAGATATGGTACTATAATGTCCCCCATACCAAACTTGCTCAAGTTAAGGGGCACCAGAACTGGGTCAAAGTCACTGGCGAATACCTTACTTTCCCTGGTGGTGGAACACAATTCAAGCATGGTGCTCTTCATTATATAGATTGGCTACAAGAGTCTGTTTCTGATATTGCCTGGGGAAAACGATCTCGTGTCATATTAGATGTTGGATGTGGGGTTGCAAGCTTTGGAGGATATCAATTTGACAGAGATGTCCTAACAATGTCCTTTGCCCCAAAAGATGAGCATGAAGCTCAAGTGCAGTTTGCACTTGAAAGAGGAATTCCTGCTATATCTGCTGTGATGGGCACACAGAGACTTCCCTATCCTAGCAAAGTTTTTGATGTTGTCCACTGTGCCCGCTGCAGGGTTCCCTGGCATATAGAAGGTGGTAAGCTTCTATTAGAGCTGAATCGTGTGTTGAGACCTGGTGGTTTCTTTGTGTGGTCTGCTACCCCAGTATATCAGAAGAAACGTGAAGACGTTGAAATATGGGCAGCCATGAAGGAACTAACAGAGAAGATATGTTGGGAACTTGTCTCAATTAATAAGGATACAATAAATGCAGTTGGTGCAGCTATATACAGAAAACCTACCACCAATGAATGTTACGAGCAAAGATCACAAAATGAGCCTCCTATCTGTGACAAGTCTGATGATCCGAATGCAGCATGGAAAGTACCATTACAAGCATGCTTGCACAAAGTTCCTGTAGATGCGTCAGAGCGTGGGTCTCAGTGGCCTGAGCAATGGCCAGCGAGGTTGGACAAGGTACCTTATTGGTTGTTAAGTTCGCAGACTGGAGCTTACGGAAAACCAGCGCCTGAGGATTTCTCTGCAGACTATGAGCACTGGAAACGCGTGGTGAACAAGTCTTATTTAACTGGAATGGGCATCAACTGGTCATCTATTAGGAATGTCATGGACATGAGATCTGTTTATGGAGGATTTGCTGCAGCTCTGAAAGATTTGAAAGTTTGGGTCATGAATATAGTCACAATAGACTCTCCAGATACCCTGCCCATAATTTATGAGCGAGGTCTATTTGGCATGTATCATGATTGGTGTGAATCATTTAGCACCTATCCCAGGTCTTATGATCTTCTCCACGCAGACCATCTGTTTTCCCTGCTCAAAAAGAGGTGCAATTTAGTTGCTGTAGTTGCAGAGGTTGATCGAATCCTTCGACCAGAGGGAAAACTTATAGTTCGGGACAATGTTGAGACTATTAATGAGCTGGAGAGGATGTTGAAGTCTATGCAGTGGGAGGTTCGCATGACCTATTCCAAGGACAAGGAGGGATTGCTTTGTGTGCAGAAGTCCATGTGGCGGCCTAGAGAGTCGGAGACAGTCAAGTACGCCATTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

814

Amino Acids

92.53

Weight (kDa)

5.0

Isoelectric Point (pI)

43.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	293 - 798	4.2e-231	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	412 - 506	7.4e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64030
fragaria_vesca	FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543
malus_domestica	MD15G1134500.v1.1
prunus_persica	Prupe.4G273900_v2.0.a1 Prupe.4G273900_v2.0.a1
pyrus_communis	pycom15g12110 pycom505g00120
rosa_chinensis	RchiOBHm_Chr2g0121981
rosa_laevigata	RLG00000018644
rosa_multiflora	Rmu_sc0003880.1_g000014
rosa_roxburghii	Rroxscaffold_2G00122100
rosa_rugosa	Rorug02G0235700 Rorug02G0235800
rosa_samantha	Rh2AG292400 Rh2BG302900 Rh2CG281500 Rh2DG316600 Rh2DG316700 Rh4AG294200 Rh4BG300200
rosa_wichuraiana	Rw2G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	986
AasI	GACNNNNNNGTC	2 cut(s)	121, 1286
AatII	GACGTC	1 cut(s)	119
Acc36I	ACCTGC	1 cut(s)	986
Acc65I	GGTACC	1 cut(s)	1846
AccB1I	GGYRCC	3 cut(s)	130, 1089, 1846
AccI	GTMKAC	1 cut(s)	785
AccII	CGCG	1 cut(s)	1935
AccIII	TCCGGA	1 cut(s)	961
AciI	CCGC	3 cut(s)	605, 1435, 2404
AclI	AACGTT	1 cut(s)	817
AclWI	GGATC	1 cut(s)	1727
AcoI	YGGCCR	2 cut(s)	1022, 1826
AcsI	RAATTY	3 cut(s)	472, 583, 1356
AcuI	CTGAAG	3 cut(s)	339, 363, 387
AcyI	GRCGYC	2 cut(s)	44, 116
AfaI	GTAC	5 cut(s)	928, 1049, 1755, 1848, 2432
AfiI	CCNNNNNNNGG	7 cut(s)	204, 1099, 1139, 1440, 1441, 2163, 2403
AflIII	ACRYGT	1 cut(s)	1933
AjnI	CCWGG	5 cut(s)	1132, 1208, 1448, 1501, 2162
AluBI	AGCT	9 cut(s)	739, 1260, 1328, 1471, 1483, 1652, 1880, 2033, 2311
AluI	AGCT	9 cut(s)	739, 1260, 1328, 1471, 1483, 1652, 1880, 2033, 2311
Alw21I	GWGCWC	2 cut(s)	1165, 1926
Alw26I	GTCTC	7 cut(s)	743, 1384, 1492, 1618, 1812, 2291, 2414
AlwI	GGATC	1 cut(s)	1727
AlwNI	CAGNNNCTG	1 cut(s)	1898
Ama87I	CYCGRG	1 cut(s)	305
Aor13HI	TCCGGA	1 cut(s)	961
AoxI	GGCC	5 cut(s)	872, 1022, 1814, 1826, 2405
ApeKI	GCWGC	7 cut(s)	284, 1437, 1571, 1649, 1742, 2027, 2030
ApoI	RAATTY	3 cut(s)	472, 583, 1356
AseI	ATTAAT	2 cut(s)	1620, 2304
Asp718I	GGTACC	1 cut(s)	1846
AspLEI	GCGC	1 cut(s)	1897
AspS9I	GGNCC	1 cut(s)	872
AsuC2I	CCSGG	1 cut(s)	237
AsuHPI	GGTGA	7 cut(s)	76, 187, 251, 322, 515, 548, 1951
AvaI	CYCGRG	1 cut(s)	305
AxyI	CCTNAGG	1 cut(s)	1899
BaeGI	GKGCMC	3 cut(s)	1092, 1385, 1435
BalI	TGGCCA	2 cut(s)	1024, 1828
BanI	GGYRCC	3 cut(s)	130, 1089, 1846
BauI	CACGAG	1 cut(s)	1224
BbsI	GAAGAC	3 cut(s)	410, 911, 1560
Bbv12I	GWGCWC	2 cut(s)	1165, 1926
BbvI	GCAGC	7 cut(s)	271, 1424, 1583, 1661, 1754, 2014, 2042
BccI	CCATC	6 cut(s)	389, 413, 476, 494, 1372, 2199
BciT130I	CCWGG	5 cut(s)	1134, 1210, 1450, 1503, 2164
BciVI	GTATCC	1 cut(s)	1621
BcnI	CCSGG	1 cut(s)	237
BcoDI	GTCTC	7 cut(s)	743, 1384, 1492, 1618, 1812, 2291, 2414
BfaI	CTAG	2 cut(s)	1404, 2409
BfmI	CTRYAG	5 cut(s)	1438, 1785, 1911, 2028, 2232
BfoI	RGCGCY	1 cut(s)	1898
BfuAI	ACCTGC	1 cut(s)	986
BfuI	GTATCC	1 cut(s)	1621
BglII	AGATCT	1 cut(s)	2006
BisI	GCNGC	8 cut(s)	285, 1438, 1572, 1650, 1743, 2028, 2031, 2405
BlsI	GCNGC	8 cut(s)	286, 1439, 1573, 1651, 1744, 2029, 2032, 2406
Bme1390I	CCNGG	6 cut(s)	237, 1134, 1210, 1450, 1503, 2164
BmeT110I	CYCGRG	1 cut(s)	305
BmgT120I	GGNCC	1 cut(s)	872
BmiI	GGNNCC	5 cut(s)	132, 969, 1091, 1446, 1848
BmrFI	CCNGG	6 cut(s)	237, 1134, 1210, 1450, 1503, 2164
BmrI	ACTGGG	2 cut(s)	1108, 1526
BmsI	GCATC	4 cut(s)	244, 592, 1780, 1977
BmuI	ACTGGG	2 cut(s)	1108, 1526
BpiI	GAAGAC	3 cut(s)	410, 911, 1560
BplI	GAGNNNNNCTC	2 cut(s)	1892, 1924
BpmI	CTGGAG	3 cut(s)	1896, 2067, 2333
Bpu10I	CCTNAGC	2 cut(s)	288, 1818
BpuEI	CTTGAG	2 cut(s)	1062, 1314
BpuMI	CCSGG	1 cut(s)	237
BsaHI	GRCGYC	2 cut(s)	44, 116
BsaI	GGTCTC	2 cut(s)	1492, 1812
BsaJI	CCNNGG	7 cut(s)	235, 306, 1132, 1209, 1448, 2162, 2361
BsaWI	WCCGGW	2 cut(s)	247, 961
BsaXI	ACNNNNNCTCC	4 cut(s)	431, 461, 2306, 2336
Bsc4I	CCNNNNNNNGG	7 cut(s)	204, 1099, 1139, 1440, 1441, 2163, 2403
Bse118I	RCCGGY	1 cut(s)	133
Bse1I	ACTGG	7 cut(s)	1103, 1117, 1532, 1879, 1931, 1963, 1979
Bse21I	CCTNAGG	1 cut(s)	1899
Bse3DI	GCAATG	2 cut(s)	1829, 2436
BseAI	TCCGGA	1 cut(s)	961
BseBI	CCWGG	5 cut(s)	1134, 1210, 1450, 1503, 2164
BseDI	CCNNGG	7 cut(s)	235, 306, 1132, 1209, 1448, 2162, 2361
BseGI	GGATG	8 cut(s)	114, 259, 526, 562, 607, 882, 1250, 2325
BseLI	CCNNNNNNNGG	7 cut(s)	204, 1099, 1139, 1440, 1441, 2163, 2403
BseMI	GCAATG	2 cut(s)	1829, 2436
BseMII	CTCAG	6 cut(s)	302, 743, 794, 1809, 1823, 1890
BseNI	ACTGG	7 cut(s)	1103, 1117, 1532, 1879, 1931, 1963, 1979
BseRI	GAGGAG	4 cut(s)	347, 395, 452, 980
BseSI	GKGCMC	3 cut(s)	1092, 1385, 1435
BseXI	GCAGC	7 cut(s)	271, 1424, 1583, 1661, 1754, 2014, 2042
BseYI	CCCAGC	1 cut(s)	869
BsgI	GTGCAG	3 cut(s)	1355, 1668, 2408
Bsh1236I	CGCG	1 cut(s)	1935
BshFI	GGCC	5 cut(s)	874, 1024, 1816, 1828, 2407
BshNI	GGYRCC	3 cut(s)	130, 1089, 1846
BsiHKAI	GWGCWC	2 cut(s)	1165, 1926
BsiHKCI	CYCGRG	1 cut(s)	305
BsiSI	CCGG	4 cut(s)	134, 237, 248, 962
BslFI	GGGAC	2 cut(s)	1043, 2300
BslI	CCNNNNNNNGG	7 cut(s)	204, 1099, 1139, 1440, 1441, 2163, 2403
BsmAI	GTCTC	7 cut(s)	743, 1384, 1492, 1618, 1812, 2291, 2414
BsmFI	GGGAC	2 cut(s)	1043, 2300
BsmI	GAATGC	1 cut(s)	1744
BsnI	GGCC	5 cut(s)	874, 1024, 1816, 1828, 2407
Bso31I	GGTCTC	2 cut(s)	1492, 1812
BsoBI	CYCGRG	1 cut(s)	305
Bsp1286I	GDGCHC	5 cut(s)	1092, 1165, 1385, 1435, 1926
Bsp13I	TCCGGA	1 cut(s)	961
Bsp143I	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
BspACI	CCGC	3 cut(s)	605, 1435, 2404
BspANI	GGCC	5 cut(s)	874, 1024, 1816, 1828, 2407
BspCNI	CTCAG	6 cut(s)	301, 742, 793, 1810, 1822, 1891
BspEI	TCCGGA	1 cut(s)	961
BspFNI	CGCG	1 cut(s)	1935
BspHI	TCATGA	2 cut(s)	2058, 2131
BspLI	GGNNCC	5 cut(s)	132, 969, 1091, 1446, 1848
BspMAI	CTGCAG	3 cut(s)	1442, 1915, 2032
BspMI	ACCTGC	1 cut(s)	986
BspPI	GGATC	1 cut(s)	1727
BspQI	GCTCTTC	1 cut(s)	1170
BspT107I	GGYRCC	3 cut(s)	130, 1089, 1846
BspTNI	GGTCTC	2 cut(s)	1492, 1812
BsrDI	GCAATG	2 cut(s)	1829, 2436
BsrFI	RCCGGY	1 cut(s)	133
BsrI	ACTGG	7 cut(s)	1103, 1117, 1532, 1879, 1931, 1963, 1979
BssAI	RCCGGY	1 cut(s)	133
BssECI	CCNNGG	7 cut(s)	235, 306, 1132, 1209, 1448, 2162, 2361
BssMI	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
BssNI	GRCGYC	2 cut(s)	44, 116
BssSI	CACGAG	1 cut(s)	1224
BssT1I	CCWWGG	1 cut(s)	2361
Bst2BI	CACGAG	1 cut(s)	1224
Bst2UI	CCWGG	5 cut(s)	1134, 1210, 1450, 1503, 2164
Bst4CI	ACNGT	6 cut(s)	64, 691, 926, 959, 1430, 2425
Bst6I	CTCTTC	6 cut(s)	291, 315, 339, 363, 996, 1170
BstACI	GRCGYC	2 cut(s)	44, 116
BstAPI	GCANNNNNTGC	1 cut(s)	1646
BstC8I	GCNNGC	6 cut(s)	906, 1258, 1435, 1768, 1772, 1830
BstDEI	CTNAG	7 cut(s)	288, 450, 729, 780, 1809, 1818, 1899
BstEII	GGTNACC	1 cut(s)	64
BstENI	CCTNNNNNAGG	1 cut(s)	2161
BstF5I	GGATG	8 cut(s)	114, 259, 526, 562, 607, 882, 1250, 2325
BstFNI	CGCG	1 cut(s)	1935
BstH2I	RGCGCY	1 cut(s)	1898
BstHHI	GCGC	1 cut(s)	1897
BstKTI	GATC	6 cut(s)	1223, 1697, 1735, 2009, 2176, 2251
BstMAI	GTCTC	7 cut(s)	743, 1384, 1492, 1618, 1812, 2291, 2414
BstMBI	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
BstMWI	GCNNNNNNNGC	4 cut(s)	1370, 1646, 2237, 2386
BstNI	CCWGG	5 cut(s)	1134, 1210, 1450, 1503, 2164
BstNSI	RCATGY	2 cut(s)	1770, 2128
BstPI	GGTNACC	1 cut(s)	64
BstSCI	CCNGG	6 cut(s)	235, 1132, 1208, 1448, 1501, 2162
BstSFI	CTRYAG	5 cut(s)	1438, 1785, 1911, 2028, 2232
BstSLI	GKGCMC	3 cut(s)	1092, 1385, 1435
BstUI	CGCG	1 cut(s)	1935
BstV1I	GCAGC	7 cut(s)	271, 1424, 1583, 1661, 1754, 2014, 2042
BstV2I	GAAGAC	3 cut(s)	410, 911, 1560
BstX2I	RGATCY	1 cut(s)	2006
BstXI	CCANNNNNNTGG	1 cut(s)	1020
BstYI	RGATCY	1 cut(s)	2006
Bsu36I	CCTNAGG	1 cut(s)	1899
BsuI	GTATCC	1 cut(s)	1621
BsuRI	GGCC	5 cut(s)	874, 1024, 1816, 1828, 2407
BtsCI	GGATG	8 cut(s)	114, 259, 526, 562, 607, 882, 1250, 2325
BtsI	GCAGTG	1 cut(s)	2342
BtsIMutI	CAGTG	6 cut(s)	955, 1110, 1426, 1817, 1924, 2342
BveI	ACCTGC	1 cut(s)	986
Cac8I	GCNNGC	6 cut(s)	906, 1258, 1435, 1768, 1772, 1830
CaiI	CAGNNNCTG	1 cut(s)	1898
CciI	TCATGA	2 cut(s)	2058, 2131
CfoI	GCGC	1 cut(s)	1897
Cfr10I	RCCGGY	1 cut(s)	133
Cfr13I	GGNCC	1 cut(s)	872
CseI	GACGC	2 cut(s)	33, 1782
CsiI	ACCWGGT	1 cut(s)	1501
Csp6I	GTAC	5 cut(s)	927, 1048, 1754, 1847, 2431
CviQI	GTAC	5 cut(s)	927, 1048, 1754, 1847, 2431
DdeI	CTNAG	7 cut(s)	288, 450, 729, 780, 1809, 1818, 1899
DpnI	GATC	6 cut(s)	1222, 1696, 1734, 2008, 2175, 2250
DpnII	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
DrdI	GACNNNNNNGTC	2 cut(s)	121, 1286
DseDI	GACNNNNNNGTC	2 cut(s)	121, 1286
EaeI	YGGCCR	2 cut(s)	1022, 1826
Eam1104I	CTCTTC	6 cut(s)	291, 315, 339, 363, 996, 1170
EarI	CTCTTC	6 cut(s)	291, 315, 339, 363, 996, 1170
Eco130I	CCWWGG	1 cut(s)	2361
Eco31I	GGTCTC	2 cut(s)	1492, 1812
Eco32I	GATATC	1 cut(s)	1271
Eco57I	CTGAAG	3 cut(s)	339, 363, 387
Eco81I	CCTNAGG	1 cut(s)	1899
Eco88I	CYCGRG	1 cut(s)	305
Eco91I	GGTNACC	1 cut(s)	64
EcoNI	CCTNNNNNAGG	1 cut(s)	2161
EcoO65I	GGTNACC	1 cut(s)	64
EcoRI	GAATTC	3 cut(s)	472, 583, 1356
EcoRII	CCWGG	5 cut(s)	1132, 1208, 1448, 1501, 2162
EcoRV	GATATC	1 cut(s)	1271
EcoT14I	CCWWGG	1 cut(s)	2361
ErhI	CCWWGG	1 cut(s)	2361
FaqI	GGGAC	2 cut(s)	1043, 2300
FauI	CCCGC	1 cut(s)	1442
FblI	GTMKAC	1 cut(s)	785
Fnu4HI	GCNGC	8 cut(s)	285, 1438, 1572, 1650, 1743, 2028, 2031, 2405
FokI	GGATG	8 cut(s)	121, 266, 533, 569, 614, 869, 1257, 2332
Fsp4HI	GCNGC	8 cut(s)	285, 1438, 1572, 1650, 1743, 2028, 2031, 2405
FspBI	CTAG	2 cut(s)	1404, 2409
GlaI	GCGC	1 cut(s)	1896
GluI	GCNGC	8 cut(s)	285, 1438, 1572, 1650, 1743, 2028, 2031, 2405
GsaI	CCCAGC	1 cut(s)	873
GsuI	CTGGAG	3 cut(s)	1896, 2067, 2333
HaeII	RGCGCY	1 cut(s)	1898
HaeIII	GGCC	5 cut(s)	874, 1024, 1816, 1828, 2407
HapII	CCGG	4 cut(s)	134, 237, 248, 962
HgaI	GACGC	2 cut(s)	33, 1782
HhaI	GCGC	1 cut(s)	1897
Hin1I	GRCGYC	2 cut(s)	44, 116
Hin6I	GCGC	1 cut(s)	1895
HinP1I	GCGC	1 cut(s)	1895
HincII	GTYRAC	2 cut(s)	777, 786
HindII	GTYRAC	2 cut(s)	777, 786
HindIII	AAGCTT	2 cut(s)	1258, 1469
HpaII	CCGG	4 cut(s)	134, 237, 248, 962
HphI	GGTGA	7 cut(s)	76, 187, 251, 322, 515, 548, 1951
Hpy166II	GTNNAC	5 cut(s)	694, 777, 786, 1426, 1942
Hpy188III	TCNNGA	9 cut(s)	158, 356, 947, 962, 995, 2059, 2084, 2132, 2285
Hpy8I	GTNNAC	5 cut(s)	694, 777, 786, 1426, 1942
Hpy99I	CGWCG	6 cut(s)	49, 64, 121, 124, 127, 262
HpyCH4III	ACNGT	6 cut(s)	64, 691, 926, 959, 1430, 2425
HpyCH4IV	ACGT	4 cut(s)	116, 817, 1549, 1557
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1370, 1646, 2237, 2386
HpyF3I	CTNAG	7 cut(s)	288, 450, 729, 780, 1809, 1818, 1899
HpySE526I	ACGT	4 cut(s)	116, 817, 1549, 1557
Hsp92I	GRCGYC	2 cut(s)	44, 116
HspAI	GCGC	1 cut(s)	1895
Kpn2I	TCCGGA	1 cut(s)	961
KpnI	GGTACC	1 cut(s)	1850
Kzo9I	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
LguI	GCTCTTC	1 cut(s)	1170
LmnI	GCTCC	4 cut(s)	181, 655, 967, 1877
Lsp1109I	GCAGC	7 cut(s)	271, 1424, 1583, 1661, 1754, 2014, 2042
LweI	GCATC	4 cut(s)	244, 592, 1780, 1977
MabI	ACCWGGT	1 cut(s)	1501
MaeI	CTAG	2 cut(s)	1404, 2409
MaeII	ACGT	4 cut(s)	116, 817, 1549, 1557
MaeIII	GTNAC	8 cut(s)	50, 64, 503, 536, 1108, 1682, 1718, 2068
MalI	GATC	6 cut(s)	1222, 1696, 1734, 2008, 2175, 2250
MboI	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
MfeI	CAATTG	2 cut(s)	844, 1014
MflI	RGATCY	1 cut(s)	2006
MhlI	GDGCHC	5 cut(s)	1092, 1165, 1385, 1435, 1926
MlsI	TGGCCA	2 cut(s)	1024, 1828
MluI	ACGCGT	1 cut(s)	1933
MluNI	TGGCCA	2 cut(s)	1024, 1828
MlyI	GAGTC	6 cut(s)	455, 800, 925, 1199, 2072, 2423
MmeI	TCCRAC	3 cut(s)	1222, 1818, 2397
Mox20I	TGGCCA	2 cut(s)	1024, 1828
MroI	TCCGGA	1 cut(s)	961
MscI	TGGCCA	2 cut(s)	1024, 1828
MseI	TTAA	6 cut(s)	1083, 1620, 1862, 1955, 2304, 2443
MslI	CAYNNNNRTG	2 cut(s)	74, 2000
Msp20I	TGGCCA	2 cut(s)	1024, 1828
MspA1I	CMGCKG	1 cut(s)	1437
MspI	CCGG	4 cut(s)	134, 237, 248, 962
MspR9I	CCNGG	6 cut(s)	237, 1134, 1210, 1450, 1503, 2164
MunI	CAATTG	2 cut(s)	844, 1014
Mva1269I	GAATGC	1 cut(s)	1744
MvaI	CCWGG	5 cut(s)	1134, 1210, 1450, 1503, 2164
MvnI	CGCG	1 cut(s)	1935
MwoI	GCNNNNNNNGC	4 cut(s)	1370, 1646, 2237, 2386
NciI	CCSGG	1 cut(s)	237
NdeII	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
NlaIV	GGNNCC	5 cut(s)	132, 969, 1091, 1446, 1848
NmuCI	GTSAC	6 cut(s)	64, 503, 536, 1108, 1718, 2068
NspI	RCATGY	2 cut(s)	1770, 2128
PaeI	GCATGC	1 cut(s)	1770
PagI	TCATGA	2 cut(s)	2058, 2131
PaqCI	CACCTGC	1 cut(s)	986
PciSI	GCTCTTC	1 cut(s)	1170
PcsI	WCGNNNNNNNCGW	1 cut(s)	2257
PctI	GAATGC	1 cut(s)	1744
PfeI	GAWTC	5 cut(s)	467, 524, 1486, 2144, 2253
PflFI	GACNNNGTC	2 cut(s)	118, 790
PkrI	GCNGC	8 cut(s)	286, 1439, 1573, 1651, 1744, 2029, 2032, 2406
PleI	GAGTC	6 cut(s)	454, 799, 924, 1198, 2072, 2422
PpsI	GAGTC	6 cut(s)	454, 799, 924, 1198, 2072, 2422
PshBI	ATTAAT	2 cut(s)	1620, 2304
Psp1406I	AACGTT	1 cut(s)	817
Psp6I	CCWGG	5 cut(s)	1132, 1208, 1448, 1501, 2162
PspEI	GGTNACC	1 cut(s)	64
PspFI	CCCAGC	1 cut(s)	869
PspGI	CCWGG	5 cut(s)	1132, 1208, 1448, 1501, 2162
PspN4I	GGNNCC	5 cut(s)	132, 969, 1091, 1446, 1848
PspPI	GGNCC	1 cut(s)	872
PstI	CTGCAG	3 cut(s)	1442, 1915, 2032
PstNI	CAGNNNCTG	1 cut(s)	1898
PsuI	RGATCY	1 cut(s)	2006
PsyI	GACNNNGTC	2 cut(s)	118, 790
RsaI	GTAC	5 cut(s)	928, 1049, 1755, 1848, 2432
RsaNI	GTAC	5 cut(s)	927, 1048, 1754, 1847, 2431
RseI	CAYNNNNRTG	2 cut(s)	74, 2000
SalI	GTCGAC	1 cut(s)	784
SapI	GCTCTTC	1 cut(s)	1170
SaqAI	TTAA	6 cut(s)	1083, 1620, 1862, 1955, 2304, 2443
SatI	GCNGC	8 cut(s)	285, 1438, 1572, 1650, 1743, 2028, 2031, 2405
Sau3AI	GATC	6 cut(s)	1220, 1694, 1732, 2006, 2173, 2248
Sau96I	GGNCC	1 cut(s)	872
SchI	GAGTC	6 cut(s)	455, 800, 925, 1199, 2072, 2423
ScrFI	CCNGG	6 cut(s)	237, 1134, 1210, 1450, 1503, 2164
SduI	GDGCHC	5 cut(s)	1092, 1165, 1385, 1435, 1926
SexAI	ACCWGGT	1 cut(s)	1501
SfaNI	GCATC	4 cut(s)	244, 592, 1780, 1977
SfcI	CTRYAG	5 cut(s)	1438, 1785, 1911, 2028, 2232
SmiMI	CAYNNNNRTG	2 cut(s)	74, 2000
SmlI	CTYRAG	2 cut(s)	1077, 1329
SmoI	CTYRAG	2 cut(s)	1077, 1329
SphI	GCATGC	1 cut(s)	1770
SsiI	CCGC	3 cut(s)	605, 1435, 2404
SspI	AATATT	1 cut(s)	619
SspMI	CTAG	2 cut(s)	1404, 2409
StyD4I	CCNGG	6 cut(s)	235, 1132, 1208, 1448, 1501, 2162
StyI	CCWWGG	1 cut(s)	2361
TaaI	ACNGT	6 cut(s)	64, 691, 926, 959, 1430, 2425
TaiI	ACGT	4 cut(s)	119, 820, 1552, 1560
TaqI	TCGA	6 cut(s)	47, 59, 470, 785, 2251, 2260
TauI	GCSGC	1 cut(s)	2407
TfiI	GAWTC	5 cut(s)	467, 524, 1486, 2144, 2253
Tru1I	TTAA	6 cut(s)	1083, 1620, 1862, 1955, 2304, 2443
Tru9I	TTAA	6 cut(s)	1083, 1620, 1862, 1955, 2304, 2443
TscAI	CASTG	6 cut(s)	962, 1117, 1433, 1817, 1931, 2342
TseFI	GTSAC	6 cut(s)	64, 503, 536, 1108, 1718, 2068
TseI	GCWGC	7 cut(s)	284, 1437, 1571, 1649, 1742, 2027, 2030
Tsp45I	GTSAC	6 cut(s)	64, 503, 536, 1108, 1718, 2068
TspGWI	ACGGA	3 cut(s)	428, 593, 1899
TspRI	CASTG	6 cut(s)	962, 1117, 1433, 1817, 1931, 2342
Tth111I	GACNNNGTC	2 cut(s)	118, 790
VspI	ATTAAT	2 cut(s)	1620, 2304
XagI	CCTNNNNNAGG	1 cut(s)	2161
XapI	RAATTY	3 cut(s)	472, 583, 1356
XceI	RCATGY	2 cut(s)	1770, 2128
XmiI	GTMKAC	1 cut(s)	785
XspI	CTAG	2 cut(s)	1404, 2409
ZraI	GACGTC	1 cut(s)	117

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.