Rh4AG294200

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007018
Physical Location & Seq
Reverse (-)
60775887 .. 60777678
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG294200.1

Sequence Viewer

Length: 690 bp
ATGAAGGAACTAACAAAGAAGATATGTTGGGAACTTGTCTCAATTAATAAGGATACAATAAATGCAGTTGGTGCAGCTATATACAGAAAACCTACCACCAATGAATGTTACGAGCAAAGATCACAAAATGAGCCTCCTATCTGTGACAAGTCTGATGATCCGAATGCAGCATGGAAAGTACCATTACAAGCATGCTTGCACAAAGTTCCTGTAGATGCGTCAGAGCGTGGGTCTCAGTGGCCTGAGCAATGGCCAGCGAGGTTGGACAAGGTACCTTACTGGTTGTTAAGTTCGCAGACTGGAGCTTACGGAAAACCAGCGCCTGAGGATTTCGCTGCAGACTATGAGCACTGGAAACGCGTGGTGAACAAGTCTTATTTAACTGGAATGGGCATTAACTGGTCATCTATTAGGAATGTCATGGACATGAGATCTGTATATGGAGGATTTGCTGCGGCTCTGAAAGATTTGAAAGTTTGGGTCATGAATATAGTCACAATAGACTCTCCTGATTTTCCAAATTCTTATGGAATCTCAATTGTCACCAACTTGATGAGGAAAAAGATTATGAAAGCGAAGTTCAGACTTCCTCCACTCTCAGATGCAACACCAGCACCTCAACACACCCGCTCATTGTTTTTAGGAAGTTTCTTTTGCACATGTGGTGAAGGAACTAATGTCCATAAATAG

Protein Analysis

229

Amino Acids

25.83

Weight (kDa)

8.95

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 1 - 171 8.7e-53 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 271
AccBSI CCGCTC 1 cut(s) 630
AccII CGCG 1 cut(s) 360
AciI CCGC 2 cut(s) 455, 628
AclWI GGATC 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 2 cut(s) 180, 273
AflIII ACRYGT 2 cut(s) 358, 659
AgsI TTSAA 1 cut(s) 472
AluBI AGCT 2 cut(s) 77, 305
AluI AGCT 2 cut(s) 77, 305
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 43, 237
AlwI GGATC 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 2 cut(s) 239, 251
ApeKI GCWGC 4 cut(s) 74, 167, 335, 452
ApoI RAATTY 1 cut(s) 520
AseI ATTAAT 1 cut(s) 45
Asp718I GGTACC 1 cut(s) 271
AspLEI GCGC 1 cut(s) 322
AsuHPI GGTGA 3 cut(s) 376, 535, 677
AxyI CCTNAGG 1 cut(s) 324
BalI TGGCCA 1 cut(s) 253
BanI GGYRCC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 86, 179, 322, 439
BciVI GTATCC 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 43, 237
BfmI CTRYAG 2 cut(s) 210, 336
BfoI RGCGCY 1 cut(s) 323
BfuI GTATCC 1 cut(s) 46
BglII AGATCT 1 cut(s) 431
BisI GCNGC 5 cut(s) 75, 168, 336, 453, 456
BlsI GCNGC 5 cut(s) 76, 169, 337, 454, 457
BmiI GGNNCC 1 cut(s) 273
BmsI GCATC 2 cut(s) 205, 592
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 237
Bse1I ACTGG 5 cut(s) 284, 304, 356, 388, 404
Bse21I CCTNAGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 254
BseMII CTCAG 4 cut(s) 234, 248, 315, 612
BseNI ACTGG 5 cut(s) 284, 304, 356, 388, 404
BseXI GCAGC 4 cut(s) 86, 179, 322, 439
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 2 cut(s) 241, 253
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 351
BsmAI GTCTC 2 cut(s) 43, 237
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 2 cut(s) 241, 253
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 3 cut(s) 119, 157, 431
BspACI CCGC 2 cut(s) 455, 628
BspANI GGCC 2 cut(s) 241, 253
BspCNI CTCAG 4 cut(s) 235, 247, 316, 611
BspFNI CGCG 1 cut(s) 360
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 271
BspTNI GGTCTC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 254
BsrI ACTGG 5 cut(s) 284, 304, 356, 388, 404
BssMI GATC 3 cut(s) 119, 157, 431
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 193, 197, 255
BstDEI CTNAG 4 cut(s) 234, 243, 324, 598
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 3 cut(s) 122, 160, 434
BstMAI GTCTC 2 cut(s) 43, 237
BstMBI GATC 3 cut(s) 119, 157, 431
BstMWI GCNNNNNNNGC 2 cut(s) 71, 611
BstNSI RCATGY 2 cut(s) 195, 663
BstSFI CTRYAG 2 cut(s) 210, 336
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 4 cut(s) 86, 179, 322, 439
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 324
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 2 cut(s) 241, 253
BtsIMutI CAGTG 2 cut(s) 242, 349
Cac8I GCNNGC 3 cut(s) 193, 197, 255
CaiI CAGNNNCTG 1 cut(s) 323
CciI TCATGA 1 cut(s) 483
CfoI GCGC 1 cut(s) 322
CseI GACGC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 179, 272
CviAII CATG 6 cut(s) 171, 192, 421, 427, 484, 660
CviJI RGCY 6 cut(s) 77, 133, 241, 253, 305, 458
CviKI_1 RGCY 6 cut(s) 77, 133, 241, 253, 305, 458
CviQI GTAC 2 cut(s) 179, 272
DdeI CTNAG 4 cut(s) 234, 243, 324, 598
DpnI GATC 3 cut(s) 121, 159, 433
DpnII GATC 3 cut(s) 119, 157, 431
EaeI YGGCCR 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 324
FaeI CATG 6 cut(s) 174, 195, 424, 430, 487, 663
FatI CATG 6 cut(s) 170, 191, 420, 426, 483, 659
FauI CCCGC 1 cut(s) 635
Fnu4HI GCNGC 5 cut(s) 75, 168, 336, 453, 456
Fsp4HI GCNGC 5 cut(s) 75, 168, 336, 453, 456
GlaI GCGC 1 cut(s) 321
GluI GCNGC 5 cut(s) 75, 168, 336, 453, 456
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 2 cut(s) 241, 253
HgaI GACGC 1 cut(s) 207
HhaI GCGC 1 cut(s) 322
Hin1II CATG 6 cut(s) 174, 195, 424, 430, 487, 663
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 2 cut(s) 503, 531
HphI GGTGA 3 cut(s) 376, 535, 677
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 6 cut(s) 154, 162, 223, 462, 584, 601
Hpy188III TCNNGA 2 cut(s) 484, 509
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 1 cut(s) 662
HpyCH4V TGCA 7 cut(s) 65, 74, 167, 199, 338, 605, 657
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 611
HpyF3I CTNAG 4 cut(s) 234, 243, 324, 598
Hsp92II CATG 6 cut(s) 174, 195, 424, 430, 487, 663
HspAI GCGC 1 cut(s) 320
KpnI GGTACC 1 cut(s) 275
Kzo9I GATC 3 cut(s) 119, 157, 431
LmnI GCTCC 1 cut(s) 302
Lsp1109I GCAGC 4 cut(s) 86, 179, 322, 439
LweI GCATC 2 cut(s) 205, 592
MaeIII GTNAC 4 cut(s) 107, 143, 493, 541
MalI GATC 3 cut(s) 121, 159, 433
MbiI CCGCTC 1 cut(s) 630
MboI GATC 3 cut(s) 119, 157, 431
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 3 cut(s) 42, 520, 537
MluI ACGCGT 1 cut(s) 358
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 7 cut(s) 144, 252, 319, 437, 549, 600, 627
Mox20I TGGCCA 1 cut(s) 253
MscI TGGCCA 1 cut(s) 253
MseI TTAA 4 cut(s) 45, 287, 380, 396
MslI CAYNNNNRTG 1 cut(s) 425
Msp20I TGGCCA 1 cut(s) 253
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 169
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 71, 611
NdeII GATC 3 cut(s) 119, 157, 431
NlaIII CATG 6 cut(s) 174, 195, 424, 430, 487, 663
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 3 cut(s) 143, 493, 541
NspI RCATGY 2 cut(s) 195, 663
PaeI GCATGC 1 cut(s) 195
PagI TCATGA 1 cut(s) 483
PciI ACATGT 1 cut(s) 659
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 5 cut(s) 76, 169, 337, 454, 457
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 659
PshBI ATTAAT 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 273
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 2 cut(s) 180, 273
RsaNI GTAC 2 cut(s) 179, 272
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 4 cut(s) 45, 287, 380, 396
SatI GCNGC 5 cut(s) 75, 168, 336, 453, 456
Sau3AI GATC 3 cut(s) 119, 157, 431
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 1 cut(s) 351
SetI ASST 7 cut(s) 79, 94, 263, 273, 277, 307, 619
SfaNI GCATC 2 cut(s) 205, 592
SfcI CTRYAG 2 cut(s) 210, 336
SmiMI CAYNNNNRTG 1 cut(s) 425
SphI GCATGC 1 cut(s) 195
Sse9I AATT 3 cut(s) 42, 520, 537
SsiI CCGC 2 cut(s) 455, 628
TasI AATT 3 cut(s) 42, 520, 537
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 4 cut(s) 45, 287, 380, 396
Tru9I TTAA 4 cut(s) 45, 287, 380, 396
TscAI CASTG 2 cut(s) 242, 356
TseFI GTSAC 3 cut(s) 143, 493, 541
TseI GCWGC 4 cut(s) 74, 167, 335, 452
Tsp45I GTSAC 3 cut(s) 143, 493, 541
TspDTI ATGAA 4 cut(s) 17, 117, 500, 584
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 2 cut(s) 242, 356
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 1 cut(s) 520
XceI RCATGY 2 cut(s) 195, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.