Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0007018

Physical Location & Seq

Genomic Coordinates Forward (+)

33179689 .. 33184168

Gene Structure 4480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018644

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGCGTTAGGGAAGTATAGTAGAGTTGATAATAGGAGGTCGGCGTCGAGTTACTGCTCGACGGTGACCATTGTGGTGTTTGTGGCATTGTGCTTGGTGGGGGTTTGGATGATGACGTCATCATCGGTGGTGCCGGTTCAGAATGTAGATGTAGGTCAGGAGAACAAGAGTGAGGTGAAGGAGCAAGTGATTGAGACCAATGAGGGGAATAACAACAGGCAGTTTGAAGATAACCCAGGGGATTTACCGGAGGATGCGACGAAAGGAGATAACAATGAGGGTGCTACTCAGGTGGAAGAGAAGCCCGAGGTGAAAACTGAAGAGAAGGTTGAGGAGAAAACTGAAGAGAAGGTTGAGGAGAAAACTGAAGATGGGTTGAAAACTGAAACGGAAGATGGAGGAAGTAAGACGGAGGAGGGAGAGTCTAAGGTGGAAAATGATGAATCGAATTCAGAAGATGGAGAGAAGAAAAGTGATGGTGACAATGAGAAAAAGAATGATTCGGGTGAGGGTGACAATGAAAAGAAATCGGATGATGACAATGAGAAGAAAATGGAGAATTCTGATGAAACAAAGGATGCAGAGAAGGAGATTATTCAGATTGACGAGAAAGTGGAAAAAACTGATAAGGAGCAAGATAGTGAGAAATCGGATAATGGGCAGACTGTAAACCAGACTTCAAATGAGGTATTTCCTTCTGTTGCTCAGTCAGAGCTTTTGAATGAGACTGCAACCCAAAATGGGTCATGGTCAACTCAGTCGACAGAGTCAAAGAATGAGAAGGAGGCTCAACGTTCTTCTGACCAGCAAACTGGTTACAACTGGAAACTTTGTAACTCAACTGCTGGGCCTGATTTCATCCCATGCCTTGACAATTTGCAAGCCATTAGGAGTCTTCACAGTACCAAGCATTATGAACATCGGGAGAGGCACTGTCCGGAGGAGCCTCCCACCTGCCTCCTTCCTCTTCCAGAAGGATATAAACGCCCAATTGAGTGGCCAATGAGCAGGGAAAAGATATGGTACTATAATGTCCCCCATACCAAACTTGCTCAAATTAAGGGGCACCAGAACTGGGTCAAAGTCACTGGCGAATACCTTACTTTCCCTGGTGGTGGAACACAATTCAAGCATGGTGCTCTTCATTATATAGATTGGCTACAAGAGTCTGTTTCTGATATTGCCTGGGGAAAACGATCTCGTGTCATATTAGATGTTGGATGTGGGGTTGCAAGCTTTGGAGGATATCAATTTGACAGAGATGTCCTAACGATGTCCTTTGCCCCAAAAGATGAGCATGAAGCTCAAGTGCAGTTTGCACTTGAAAGAGGAATTCCTGCTATATCTGCTGTGATGGGCACACAGAGACTTCCCTATCCTAGCAAAGTTTTTGATGTTGTCCACTGTGCCCGCTGCAGGGTTCCCTGGCATATAGAAGGTGGTAAGCTTCTATTAGAGCTGAATCGTGTGTTGAGACCTGGTGGTTTCTTTGTGTGGTCTGCTACCCCAGTATATCAGAAGAAACGTGAAGACGTTGAAATATGGGAAGCCATGAAGGAACTAACAAAGAAGATATGTTGGGAACTTGTCTCAATTAATAAGGATACAATAAATGCAGTTGGTGCAGCTATATACAGAAAACCTACCACCAATGAATGTTACGAGCAAAGATCACAAAATGAGCCTCCTATCTGTGACAAGTCTGATGATCCGAATGCAGCATGGAAAGTACCATTACAAGCATGCTTGCACAAAGTTCCTGTAGATGCGTCAGAGCGTGGGTCTCAGTGGCCTGAGCAATGGCCAGCGAGGTTGGACAAGGTACCTTACTGGTTGTTAAGTTCGCAGACTGGAGCTTACGGAAAATCAGCTCCTGAGGATTTCGCTGCAGACTATGAGCACTGGAAACGCGTGGTGAACAAGTCTTATTTAACTGGAATGGGCATCAACTGGTCATCTATTAGGAATGTCATGGACATGAGATCTGTTTATGGAGGATTTGCTGCGGCTCTGAAAGATTTGAAAGTTTGGGTCATGAATATAGTCACAATAGACTCTCCAGATACCCTGCCCATAATTTATGAGCGAGGTCTATTTGGCATGTATCATGATTGGTGTGAATCATTTAGCACCTATCCCAGGTCTTATGATCTTCTCCACGCAGACCATCTGTTTTCCCTGCTCAAAAAGAGGTGCAATTTAGTTGCTGTAGTTGCAGAGGTTGATCGAATCCTTCGACCAGAGGGAAAACTTATAGTTCGGGACAATGTTGAGACTATTAATGAGCTGGAGAGCATGTTGAAGTCTATGCAGTGGGAGGTTCGCATGACCTATTCCAAGGACAAGGAGGGATTGCTTTGTGTGCAGAAGTCCATGTGGCGGCCTAAAGAGTCGGAGACAGTCAAGTACGCCATTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

807

Amino Acids

91.51

Weight (kDa)

5.07

Isoelectric Point (pI)

43.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	285 - 790	2.2e-231	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	404 - 498	7.3e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64030
fragaria_vesca	FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543
malus_domestica	MD15G1134500.v1.1
prunus_persica	Prupe.4G273900_v2.0.a1 Prupe.4G273900_v2.0.a1
pyrus_communis	pycom15g12110 pycom505g00120
rosa_chinensis	RchiOBHm_Chr2g0121981
rosa_laevigata	RLG00000018644
rosa_multiflora	Rmu_sc0003880.1_g000014
rosa_roxburghii	Rroxscaffold_2G00122100
rosa_rugosa	Rorug02G0235700 Rorug02G0235800
rosa_samantha	Rh2AG292400 Rh2BG302900 Rh2CG281500 Rh2DG316600 Rh2DG316700 Rh4AG294200 Rh4BG300200
rosa_wichuraiana	Rw2G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	962
AasI	GACNNNNNNGTC	1 cut(s)	1262
AatII	GACGTC	1 cut(s)	119
Acc36I	ACCTGC	1 cut(s)	962
Acc65I	GGTACC	1 cut(s)	1822
AccB1I	GGYRCC	3 cut(s)	130, 1065, 1822
AccI	GTMKAC	1 cut(s)	761
AccII	CGCG	1 cut(s)	1911
AccIII	TCCGGA	1 cut(s)	937
AciI	CCGC	3 cut(s)	1411, 2006, 2380
AclI	AACGTT	1 cut(s)	793
AclWI	GGATC	1 cut(s)	1703
AcoI	YGGCCR	2 cut(s)	998, 1802
AcsI	RAATTY	3 cut(s)	448, 559, 1332
AcuI	CTGAAG	3 cut(s)	339, 363, 387
AcyI	GRCGYC	2 cut(s)	44, 116
AfaI	GTAC	5 cut(s)	904, 1025, 1731, 1824, 2408
AfiI	CCNNNNNNNGG	8 cut(s)	204, 741, 1075, 1115, 1416, 1417, 2139, 2379
AflIII	ACRYGT	1 cut(s)	1909
AgsI	TTSAA	9 cut(s)	227, 379, 681, 721, 1129, 1325, 1538, 2023, 2302
AjnI	CCWGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
AluBI	AGCT	9 cut(s)	715, 1236, 1304, 1447, 1459, 1628, 1856, 1871, 2287
AluI	AGCT	9 cut(s)	715, 1236, 1304, 1447, 1459, 1628, 1856, 1871, 2287
Alw21I	GWGCWC	2 cut(s)	1141, 1902
Alw26I	GTCTC	8 cut(s)	188, 719, 1360, 1468, 1594, 1788, 2267, 2390
AlwI	GGATC	1 cut(s)	1703
AlwNI	CAGNNNCTG	1 cut(s)	1874
Ama87I	CYCGRG	1 cut(s)	305
Aor13HI	TCCGGA	1 cut(s)	937
AoxI	GGCC	5 cut(s)	848, 998, 1790, 1802, 2381
ApeKI	GCWGC	5 cut(s)	1413, 1625, 1718, 1886, 2003
ApoI	RAATTY	3 cut(s)	448, 559, 1332
AseI	ATTAAT	2 cut(s)	1596, 2280
Asp718I	GGTACC	1 cut(s)	1822
AspS9I	GGNCC	1 cut(s)	848
AsuHPI	GGTGA	7 cut(s)	76, 187, 322, 491, 518, 524, 1927
AvaI	CYCGRG	1 cut(s)	305
AxyI	CCTNAGG	1 cut(s)	1875
BaeGI	GKGCMC	3 cut(s)	1068, 1361, 1411
BalI	TGGCCA	2 cut(s)	1000, 1804
BanI	GGYRCC	3 cut(s)	130, 1065, 1822
BauI	CACGAG	1 cut(s)	1200
BbsI	GAAGAC	2 cut(s)	887, 1536
Bbv12I	GWGCWC	2 cut(s)	1141, 1902
BbvI	GCAGC	5 cut(s)	1400, 1637, 1730, 1873, 1990
BccI	CCATC	6 cut(s)	365, 389, 452, 470, 1348, 2175
BciT130I	CCWGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
BciVI	GTATCC	1 cut(s)	1597
BcoDI	GTCTC	8 cut(s)	188, 719, 1360, 1468, 1594, 1788, 2267, 2390
BfaI	CTAG	1 cut(s)	1380
BfmI	CTRYAG	4 cut(s)	1414, 1761, 1887, 2208
BfuAI	ACCTGC	1 cut(s)	962
BfuI	GTATCC	1 cut(s)	1597
BglII	AGATCT	1 cut(s)	1982
BisI	GCNGC	7 cut(s)	1414, 1626, 1719, 1887, 2004, 2007, 2381
BlsI	GCNGC	7 cut(s)	1415, 1627, 1720, 1888, 2005, 2008, 2382
Bme1390I	CCNGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
BmeT110I	CYCGRG	1 cut(s)	305
BmgT120I	GGNCC	1 cut(s)	848
BmiI	GGNNCC	5 cut(s)	132, 945, 1067, 1422, 1824
BmrFI	CCNGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
BmrI	ACTGGG	2 cut(s)	1084, 1502
BmsI	GCATC	4 cut(s)	244, 568, 1756, 1953
BmuI	ACTGGG	2 cut(s)	1084, 1502
BpiI	GAAGAC	2 cut(s)	887, 1536
BpmI	CTGGAG	3 cut(s)	1872, 2043, 2309
Bpu10I	CCTNAGC	1 cut(s)	1794
BpuEI	CTTGAG	1 cut(s)	1290
BsaHI	GRCGYC	2 cut(s)	44, 116
BsaI	GGTCTC	3 cut(s)	188, 1468, 1788
BsaJI	CCNNGG	8 cut(s)	235, 236, 306, 1108, 1185, 1424, 2138, 2337
BsaWI	WCCGGW	2 cut(s)	247, 937
BsaXI	ACNNNNNCTCC	4 cut(s)	407, 437, 2282, 2312
Bsc4I	CCNNNNNNNGG	8 cut(s)	204, 741, 1075, 1115, 1416, 1417, 2139, 2379
Bse118I	RCCGGY	1 cut(s)	133
Bse21I	CCTNAGG	1 cut(s)	1875
Bse3DI	GCAATG	2 cut(s)	1805, 2412
BseAI	TCCGGA	1 cut(s)	937
BseBI	CCWGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
BseDI	CCNNGG	8 cut(s)	235, 236, 306, 1108, 1185, 1424, 2138, 2337
BseGI	GGATG	6 cut(s)	114, 259, 538, 583, 858, 1226
BseLI	CCNNNNNNNGG	8 cut(s)	204, 741, 1075, 1115, 1416, 1417, 2139, 2379
BseMI	GCAATG	2 cut(s)	1805, 2412
BseMII	CTCAG	6 cut(s)	302, 719, 770, 1785, 1799, 1866
BseRI	GAGGAG	4 cut(s)	347, 371, 428, 956
BseSI	GKGCMC	3 cut(s)	1068, 1361, 1411
BseXI	GCAGC	5 cut(s)	1400, 1637, 1730, 1873, 1990
BseYI	CCCAGC	1 cut(s)	845
BsgI	GTGCAG	3 cut(s)	1331, 1644, 2384
Bsh1236I	CGCG	1 cut(s)	1911
BshFI	GGCC	5 cut(s)	850, 1000, 1792, 1804, 2383
BshNI	GGYRCC	3 cut(s)	130, 1065, 1822
BsiHKAI	GWGCWC	2 cut(s)	1141, 1902
BsiHKCI	CYCGRG	1 cut(s)	305
BsiSI	CCGG	3 cut(s)	134, 248, 938
BslFI	GGGAC	2 cut(s)	1019, 2276
BslI	CCNNNNNNNGG	8 cut(s)	204, 741, 1075, 1115, 1416, 1417, 2139, 2379
BsmAI	GTCTC	8 cut(s)	188, 719, 1360, 1468, 1594, 1788, 2267, 2390
BsmFI	GGGAC	2 cut(s)	1019, 2276
BsmI	GAATGC	1 cut(s)	1720
BsnI	GGCC	5 cut(s)	850, 1000, 1792, 1804, 2383
Bso31I	GGTCTC	3 cut(s)	188, 1468, 1788
BsoBI	CYCGRG	1 cut(s)	305
Bsp1286I	GDGCHC	5 cut(s)	1068, 1141, 1361, 1411, 1902
Bsp13I	TCCGGA	1 cut(s)	937
Bsp143I	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
BspACI	CCGC	3 cut(s)	1411, 2006, 2380
BspANI	GGCC	5 cut(s)	850, 1000, 1792, 1804, 2383
BspCNI	CTCAG	6 cut(s)	301, 718, 769, 1786, 1798, 1867
BspEI	TCCGGA	1 cut(s)	937
BspFNI	CGCG	1 cut(s)	1911
BspHI	TCATGA	2 cut(s)	2034, 2107
BspLI	GGNNCC	5 cut(s)	132, 945, 1067, 1422, 1824
BspMAI	CTGCAG	2 cut(s)	1418, 1891
BspMI	ACCTGC	1 cut(s)	962
BspPI	GGATC	1 cut(s)	1703
BspQI	GCTCTTC	1 cut(s)	1146
BspT107I	GGYRCC	3 cut(s)	130, 1065, 1822
BspTNI	GGTCTC	3 cut(s)	188, 1468, 1788
BsrDI	GCAATG	2 cut(s)	1805, 2412
BsrFI	RCCGGY	1 cut(s)	133
BssAI	RCCGGY	1 cut(s)	133
BssECI	CCNNGG	8 cut(s)	235, 236, 306, 1108, 1185, 1424, 2138, 2337
BssMI	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
BssNI	GRCGYC	2 cut(s)	44, 116
BssSI	CACGAG	1 cut(s)	1200
BssT1I	CCWWGG	1 cut(s)	2337
Bst2BI	CACGAG	1 cut(s)	1200
Bst2UI	CCWGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
Bst4CI	ACNGT	6 cut(s)	64, 667, 902, 935, 1406, 2401
Bst6I	CTCTTC	5 cut(s)	291, 315, 339, 972, 1146
BstACI	GRCGYC	2 cut(s)	44, 116
BstAPI	GCANNNNNTGC	1 cut(s)	1622
BstC8I	GCNNGC	6 cut(s)	882, 1234, 1411, 1744, 1748, 1806
BstDEI	CTNAG	7 cut(s)	288, 426, 705, 756, 1785, 1794, 1875
BstEII	GGTNACC	1 cut(s)	64
BstENI	CCTNNNNNAGG	1 cut(s)	2137
BstF5I	GGATG	6 cut(s)	114, 259, 538, 583, 858, 1226
BstFNI	CGCG	1 cut(s)	1911
BstKTI	GATC	6 cut(s)	1199, 1673, 1711, 1985, 2152, 2227
BstMAI	GTCTC	8 cut(s)	188, 719, 1360, 1468, 1594, 1788, 2267, 2390
BstMBI	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
BstMWI	GCNNNNNNNGC	4 cut(s)	1346, 1622, 2213, 2362
BstNI	CCWGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
BstNSI	RCATGY	3 cut(s)	1746, 2104, 2299
BstPI	GGTNACC	1 cut(s)	64
BstSCI	CCNGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
BstSFI	CTRYAG	4 cut(s)	1414, 1761, 1887, 2208
BstSLI	GKGCMC	3 cut(s)	1068, 1361, 1411
BstUI	CGCG	1 cut(s)	1911
BstV1I	GCAGC	5 cut(s)	1400, 1637, 1730, 1873, 1990
BstV2I	GAAGAC	2 cut(s)	887, 1536
BstX2I	RGATCY	1 cut(s)	1982
BstXI	CCANNNNNNTGG	2 cut(s)	812, 996
BstYI	RGATCY	1 cut(s)	1982
Bsu36I	CCTNAGG	1 cut(s)	1875
BsuI	GTATCC	1 cut(s)	1597
BsuRI	GGCC	5 cut(s)	850, 1000, 1792, 1804, 2383
BtsCI	GGATG	6 cut(s)	114, 259, 538, 583, 858, 1226
BtsI	GCAGTG	1 cut(s)	2318
BtsIMutI	CAGTG	6 cut(s)	931, 1086, 1402, 1793, 1900, 2318
BveI	ACCTGC	1 cut(s)	962
Cac8I	GCNNGC	6 cut(s)	882, 1234, 1411, 1744, 1748, 1806
CaiI	CAGNNNCTG	1 cut(s)	1874
CciI	TCATGA	2 cut(s)	2034, 2107
Cfr10I	RCCGGY	1 cut(s)	133
Cfr13I	GGNCC	1 cut(s)	848
CseI	GACGC	2 cut(s)	33, 1758
CsiI	ACCWGGT	1 cut(s)	1477
Csp6I	GTAC	5 cut(s)	903, 1024, 1730, 1823, 2407
CviQI	GTAC	5 cut(s)	903, 1024, 1730, 1823, 2407
DdeI	CTNAG	7 cut(s)	288, 426, 705, 756, 1785, 1794, 1875
DpnI	GATC	6 cut(s)	1198, 1672, 1710, 1984, 2151, 2226
DpnII	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
DrdI	GACNNNNNNGTC	1 cut(s)	1262
DseDI	GACNNNNNNGTC	1 cut(s)	1262
EaeI	YGGCCR	2 cut(s)	998, 1802
Eam1104I	CTCTTC	5 cut(s)	291, 315, 339, 972, 1146
EarI	CTCTTC	5 cut(s)	291, 315, 339, 972, 1146
Eco130I	CCWWGG	1 cut(s)	2337
Eco31I	GGTCTC	3 cut(s)	188, 1468, 1788
Eco32I	GATATC	1 cut(s)	1247
Eco57I	CTGAAG	3 cut(s)	339, 363, 387
Eco81I	CCTNAGG	1 cut(s)	1875
Eco88I	CYCGRG	1 cut(s)	305
Eco91I	GGTNACC	1 cut(s)	64
EcoNI	CCTNNNNNAGG	1 cut(s)	2137
EcoO65I	GGTNACC	1 cut(s)	64
EcoRI	GAATTC	3 cut(s)	448, 559, 1332
EcoRII	CCWGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
EcoRV	GATATC	1 cut(s)	1247
EcoT14I	CCWWGG	1 cut(s)	2337
ErhI	CCWWGG	1 cut(s)	2337
FaqI	GGGAC	2 cut(s)	1019, 2276
FauI	CCCGC	1 cut(s)	1418
FblI	GTMKAC	1 cut(s)	761
Fnu4HI	GCNGC	7 cut(s)	1414, 1626, 1719, 1887, 2004, 2007, 2381
FokI	GGATG	6 cut(s)	121, 266, 545, 590, 845, 1233
Fsp4HI	GCNGC	7 cut(s)	1414, 1626, 1719, 1887, 2004, 2007, 2381
FspBI	CTAG	1 cut(s)	1380
GluI	GCNGC	7 cut(s)	1414, 1626, 1719, 1887, 2004, 2007, 2381
GsaI	CCCAGC	1 cut(s)	849
GsuI	CTGGAG	3 cut(s)	1872, 2043, 2309
HaeIII	GGCC	5 cut(s)	850, 1000, 1792, 1804, 2383
HapII	CCGG	3 cut(s)	134, 248, 938
HgaI	GACGC	2 cut(s)	33, 1758
Hin1I	GRCGYC	2 cut(s)	44, 116
HincII	GTYRAC	2 cut(s)	753, 762
HindII	GTYRAC	2 cut(s)	753, 762
HindIII	AAGCTT	2 cut(s)	1234, 1445
HpaII	CCGG	3 cut(s)	134, 248, 938
HphI	GGTGA	7 cut(s)	76, 187, 322, 491, 518, 524, 1927
Hpy166II	GTNNAC	5 cut(s)	670, 753, 762, 1402, 1918
Hpy188III	TCNNGA	9 cut(s)	158, 923, 938, 971, 1874, 2035, 2060, 2108, 2261
Hpy8I	GTNNAC	5 cut(s)	670, 753, 762, 1402, 1918
Hpy99I	CGWCG	3 cut(s)	49, 64, 262
HpyCH4III	ACNGT	6 cut(s)	64, 667, 902, 935, 1406, 2401
HpyCH4IV	ACGT	4 cut(s)	116, 793, 1525, 1533
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1346, 1622, 2213, 2362
HpyF3I	CTNAG	7 cut(s)	288, 426, 705, 756, 1785, 1794, 1875
HpySE526I	ACGT	4 cut(s)	116, 793, 1525, 1533
Hsp92I	GRCGYC	2 cut(s)	44, 116
Kpn2I	TCCGGA	1 cut(s)	937
KpnI	GGTACC	1 cut(s)	1826
Kzo9I	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
LguI	GCTCTTC	1 cut(s)	1146
LmnI	GCTCC	5 cut(s)	181, 631, 943, 1853, 1876
Lsp1109I	GCAGC	5 cut(s)	1400, 1637, 1730, 1873, 1990
LweI	GCATC	4 cut(s)	244, 568, 1756, 1953
MabI	ACCWGGT	1 cut(s)	1477
MaeI	CTAG	1 cut(s)	1380
MaeII	ACGT	4 cut(s)	116, 793, 1525, 1533
MalI	GATC	6 cut(s)	1198, 1672, 1710, 1984, 2151, 2226
MboI	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
MfeI	CAATTG	1 cut(s)	990
MflI	RGATCY	1 cut(s)	1982
MhlI	GDGCHC	5 cut(s)	1068, 1141, 1361, 1411, 1902
MlsI	TGGCCA	2 cut(s)	1000, 1804
MluI	ACGCGT	1 cut(s)	1909
MluNI	TGGCCA	2 cut(s)	1000, 1804
MlyI	GAGTC	6 cut(s)	431, 776, 901, 1175, 2048, 2399
MmeI	TCCRAC	3 cut(s)	1198, 1794, 2373
Mox20I	TGGCCA	2 cut(s)	1000, 1804
MroI	TCCGGA	1 cut(s)	937
MscI	TGGCCA	2 cut(s)	1000, 1804
MseI	TTAA	6 cut(s)	1059, 1596, 1838, 1931, 2280, 2419
MslI	CAYNNNNRTG	2 cut(s)	74, 1976
Msp20I	TGGCCA	2 cut(s)	1000, 1804
MspA1I	CMGCKG	1 cut(s)	1413
MspI	CCGG	3 cut(s)	134, 248, 938
MspR9I	CCNGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
MunI	CAATTG	1 cut(s)	990
Mva1269I	GAATGC	1 cut(s)	1720
MvaI	CCWGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
MvnI	CGCG	1 cut(s)	1911
MwoI	GCNNNNNNNGC	4 cut(s)	1346, 1622, 2213, 2362
NdeII	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
NlaIV	GGNNCC	5 cut(s)	132, 945, 1067, 1422, 1824
NmuCI	GTSAC	6 cut(s)	64, 479, 512, 1084, 1694, 2044
NspI	RCATGY	3 cut(s)	1746, 2104, 2299
PaeI	GCATGC	1 cut(s)	1746
PagI	TCATGA	2 cut(s)	2034, 2107
PaqCI	CACCTGC	1 cut(s)	962
PasI	CCCWGGG	1 cut(s)	236
PciSI	GCTCTTC	1 cut(s)	1146
PcsI	WCGNNNNNNNCGW	1 cut(s)	2233
PctI	GAATGC	1 cut(s)	1720
PfeI	GAWTC	5 cut(s)	443, 500, 1462, 2120, 2229
PflFI	GACNNNGTC	1 cut(s)	766
PkrI	GCNGC	7 cut(s)	1415, 1627, 1720, 1888, 2005, 2008, 2382
PleI	GAGTC	6 cut(s)	430, 775, 900, 1174, 2048, 2398
PpsI	GAGTC	6 cut(s)	430, 775, 900, 1174, 2048, 2398
PshBI	ATTAAT	2 cut(s)	1596, 2280
Psp1406I	AACGTT	1 cut(s)	793
Psp6I	CCWGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
PspEI	GGTNACC	1 cut(s)	64
PspFI	CCCAGC	1 cut(s)	845
PspGI	CCWGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
PspN4I	GGNNCC	5 cut(s)	132, 945, 1067, 1422, 1824
PspPI	GGNCC	1 cut(s)	848
PstI	CTGCAG	2 cut(s)	1418, 1891
PstNI	CAGNNNCTG	1 cut(s)	1874
PsuI	RGATCY	1 cut(s)	1982
PsyI	GACNNNGTC	1 cut(s)	766
RsaI	GTAC	5 cut(s)	904, 1025, 1731, 1824, 2408
RsaNI	GTAC	5 cut(s)	903, 1024, 1730, 1823, 2407
RseI	CAYNNNNRTG	2 cut(s)	74, 1976
SalI	GTCGAC	1 cut(s)	760
SapI	GCTCTTC	1 cut(s)	1146
SaqAI	TTAA	6 cut(s)	1059, 1596, 1838, 1931, 2280, 2419
SatI	GCNGC	7 cut(s)	1414, 1626, 1719, 1887, 2004, 2007, 2381
Sau3AI	GATC	6 cut(s)	1196, 1670, 1708, 1982, 2149, 2224
Sau96I	GGNCC	1 cut(s)	848
SchI	GAGTC	6 cut(s)	431, 776, 901, 1175, 2048, 2399
ScrFI	CCNGG	6 cut(s)	237, 1110, 1186, 1426, 1479, 2140
SduI	GDGCHC	5 cut(s)	1068, 1141, 1361, 1411, 1902
SexAI	ACCWGGT	1 cut(s)	1477
SfaNI	GCATC	4 cut(s)	244, 568, 1756, 1953
SfcI	CTRYAG	4 cut(s)	1414, 1761, 1887, 2208
SmiMI	CAYNNNNRTG	2 cut(s)	74, 1976
SmlI	CTYRAG	1 cut(s)	1305
SmoI	CTYRAG	1 cut(s)	1305
SphI	GCATGC	1 cut(s)	1746
SsiI	CCGC	3 cut(s)	1411, 2006, 2380
SspMI	CTAG	1 cut(s)	1380
StyD4I	CCNGG	6 cut(s)	235, 1108, 1184, 1424, 1477, 2138
StyI	CCWWGG	1 cut(s)	2337
TaaI	ACNGT	6 cut(s)	64, 667, 902, 935, 1406, 2401
TaiI	ACGT	4 cut(s)	119, 796, 1528, 1536
TaqI	TCGA	6 cut(s)	47, 59, 446, 761, 2227, 2236
TauI	GCSGC	2 cut(s)	2009, 2383
TfiI	GAWTC	5 cut(s)	443, 500, 1462, 2120, 2229
Tru1I	TTAA	6 cut(s)	1059, 1596, 1838, 1931, 2280, 2419
Tru9I	TTAA	6 cut(s)	1059, 1596, 1838, 1931, 2280, 2419
TscAI	CASTG	6 cut(s)	938, 1093, 1409, 1793, 1907, 2318
TseFI	GTSAC	6 cut(s)	64, 479, 512, 1084, 1694, 2044
TseI	GCWGC	5 cut(s)	1413, 1625, 1718, 1886, 2003
Tsp45I	GTSAC	6 cut(s)	64, 479, 512, 1084, 1694, 2044
TspGWI	ACGGA	3 cut(s)	404, 425, 1875
TspRI	CASTG	6 cut(s)	938, 1093, 1409, 1793, 1907, 2318
Tth111I	GACNNNGTC	1 cut(s)	766
VspI	ATTAAT	2 cut(s)	1596, 2280
XagI	CCTNNNNNAGG	1 cut(s)	2137
XapI	RAATTY	3 cut(s)	448, 559, 1332
XceI	RCATGY	3 cut(s)	1746, 2104, 2299
XmiI	GTMKAC	1 cut(s)	761
XspI	CTAG	1 cut(s)	1380
ZraI	GACGTC	1 cut(s)	117

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.