Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007018

Physical Location & Seq

Genomic Coordinates Forward (+)

9772182 .. 9777443

Gene Structure 5262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1134500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGCGACAGGGGGAAAATATACTAGAGTAGATAATAGGAGGCCGTCCTCCGCTAGTGGTTGCTGCTCCACCGTCACCGTTGGGGTGTTTGTGGCGTTGTGCTTGGTCGGGGTTTGGATGATGACCTCCTCCTCTGTAGTTCCCATTCAGAATGTGGATGTGCCCACGGAGGAGAGTAAGAGTGAACTCAAGGAGCAGGGCAGCAATAACGTCGGCGTCAAGGAACAAGCGAGTGACACCAATGCCAGGCAATTTGAAGATAACCCGGGTGACTTACCCGACGACGCAACCAAAGGAGACAGCAGCGATGGTGCTGCTCAGGGTGGAGACAAGCTACAAGGGAAGTCCGAAGAGACGACTGAGGAGAAGTCTGTGGAAAAGACTCAGGAGACACCTGAGGAGAAAACCCAAGACAAGACTGAGGAGAAAAGTGAAGAAAAGATTGAGGAGAAAAGTGAAGAAAAGATTGAGGAGAAATCTGAAGATGGCGACTCTAAGGTGGAGAATGGAGAATTGAATTCCGAAAATGGAGAAAAAAAGAGCGATGGCGGCGAAAAGGACAAGTCTGATTCCGGTGATAATGAGAAGAAATCGGATGATGACAGTGAGAAAGTGGAGGTAACTGATACCAAGGAGTCAAATGGGGAGAAAAAGGAAAATGAGCAGGCCAAAAACCAGAGTTCAAATGAGGTATTTCCTTCCGTTGCTCAGTCGGAGCTATTGAACGAGACTACAACTCAAAATAGTTCGTGGTCAACTCAGTCGGCAGAGTCAAAGAATGAGAAGGAGGCTCAGCGTGCTTCTGATCAGAAGACCAGTTACAACTGGAAAGTCTGCAATTCCACTGCTGGGCCTGATTTCATCCCCTGCCTTGACAATTTGCAAGCCATCAAGAGTCTTCGAAGTACCAAGCATTACGAACACCGGGAGAGGCATTGTCCCGAGGAGGCCCCCAGCTGCCTTGTTCCTCTTCCAGAAGGTTATAGACGCCCAATTGAGTGGCCAACAAGCAGGGAAAAGGTATGGTACTACAATGTTCCCCATACAAAGCTTGTTCAAATTAAGGGGCATCAGAATTGGGTCAAAGTTACCGGTGAATACCTCACTTTTCCCGGTGGTGGAACACAATTCAAGCATGGTGCTCTTCATTATATTGATTTCATACAACAGTCTGTTCCTGATATTGCCTGGGGAAAACGTTCACGTGTCATATTAGATGTTGGATGCGGAGTTGCAAGCTTTGGAGGCTTTCTTTTTGACAGAGATGTCCTTGCAATGTCATTGGCCCCAAAAGATGAGCATGAAGCTCAAGTGCAGTTTGCACTTGAAAGAGGAATTCCTGCCATATCTGCTGTGATGGGCACAAAGAGACTTCCCTTCCCCGGCCAAGTTTTTGATGTTGTCCACTGTGCCCGCTGCAGGGTTCCTTGGCATATAGAAGGTGGTAAGCTTCTGTTGGAGCTGAACCGAGTGTTGAGACCTGGTGGTTTCTTTGTATGGTCTGCTACTCCAGTGTATAAGAAGCTGGGTGAAGATGTTGAAATATGGAATGCCATGAAGGAACTAACCAAATCAATATGCTGGGAACTGGTGTCAATTACTAAGGATACAGTAAATGGAGTTGGTATTGCTATATACAAAAAGCCTACTTCAAATGAGTGCTATGAGAAAAGATCACAGAACGAGCCTCCTATCTGTGCTAAGTCTGATGATCCAAATGCTGCCTGGAATGTGCCATTACAATCATGCATCCACAAAGTGCCTGTTAATGCAACAAAACGTGGGTCTGAATGGCCTGAACAATGGCCGGCGAGGTTGGACAAGGCACCTTACTGGTTGTTAAGTTCCCAAGTTGGTGTTTATGGAAAACCTGCTCCTGAGGATTTCACTTCAGACAATGAGCACTGGAAACGTGTGGTGACAAAGTCATATCTGAATGGAATGGGGATTAACTGGAAATCTGTTAGGAATGTTATGGACATGAGAGCTGTGTACGGAGGATTTGCTGCCGCTTTGAAAGATTTGAAAATTTGGGTCATGAATGTGGTCTCGGTAGACTCTCCAGACACACTACCCATAATTTACGAGCGAGGTTTGTTTGGTATGTATCATGATTGGTGTGAGTCATTTAACACCTATCCAAGGTCTTATGATCTTCTCCATGCCGACCATCTGTTTTCCAAGCTTAAGAAGAGGTGCAATTTAGTGGCTGTAGTTGCGGAGGTTGATCGAATCCTTCGACCAGAGGGAAAGCTTATCGTGCGAGACGATGTTGAGACTATTTACGAGCTGGAGAACATGGCGAGGTCGATGCAGTGGGAGGTTAGCCTGACGTATTCGAAGGACAAGGAGGGATTGCTTTGTGTGCAGAAGTCCATGTGGCGGCCCAAAGAGTCGGAGACACTCAAGTTTGCAATTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

808

Amino Acids

90.53

Weight (kDa)

5.36

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	286 - 791	4.8e-232	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25	PF13649	404 - 496	8.1e-06	Methyltransferase domain
Methyltransf_11	PF08241	405 - 499	2.1e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64030
fragaria_vesca	FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543 FvH4_1g24543
malus_domestica	MD15G1134500.v1.1
prunus_persica	Prupe.4G273900_v2.0.a1 Prupe.4G273900_v2.0.a1
pyrus_communis	pycom15g12110 pycom505g00120
rosa_chinensis	RchiOBHm_Chr2g0121981
rosa_laevigata	RLG00000018644
rosa_multiflora	Rmu_sc0003880.1_g000014
rosa_roxburghii	Rroxscaffold_2G00122100
rosa_rugosa	Rorug02G0235700 Rorug02G0235800
rosa_samantha	Rh2AG292400 Rh2BG302900 Rh2CG281500 Rh2DG316600 Rh2DG316700 Rh4AG294200 Rh4BG300200
rosa_wichuraiana	Rw2G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1265
Acc36I	ACCTGC	1 cut(s)	1879
AccB1I	GGYRCC	1 cut(s)	1825
AccI	GTMKAC	1 cut(s)	2055
AciI	CCGC	7 cut(s)	51, 549, 1227, 1414, 2010, 2219, 2383
AclI	AACGTT	1 cut(s)	1198
AclWI	GGATC	1 cut(s)	1706
AcoI	YGGCCR	3 cut(s)	1001, 1384, 1805
AcsI	RAATTY	3 cut(s)	517, 1335, 2028
AcuI	CTGAAG	2 cut(s)	501, 1875
AcvI	CACGTG	1 cut(s)	1205
AcyI	GRCGYC	2 cut(s)	216, 988
AdeI	CACNNNGTG	1 cut(s)	1759
AfaI	GTAC	3 cut(s)	907, 1028, 1994
AfiI	CCNNNNNNNGG	8 cut(s)	246, 849, 1118, 1382, 1419, 1420, 2142, 2382
AflII	CTTAAG	1 cut(s)	2186
AflIII	ACRYGT	2 cut(s)	1204, 1912
AgeI	ACCGGT	1 cut(s)	1091
AjnI	CCWGG	4 cut(s)	245, 1187, 1480, 1724
Alw21I	GWGCWC	2 cut(s)	1144, 1905
AlwI	GGATC	1 cut(s)	1706
Ama87I	CYCGRG	2 cut(s)	265, 941
ApeKI	GCWGC	8 cut(s)	63, 201, 303, 314, 957, 1416, 1721, 2006
ApoI	RAATTY	3 cut(s)	517, 1335, 2028
ArsI	GACNNNNNNTTYG	2 cut(s)	1769, 1801
AsiGI	ACCGGT	1 cut(s)	1091
Asp700I	GAANNNNTTC	1 cut(s)	1198
AspS9I	GGNCC	4 cut(s)	851, 949, 1285, 2385
AsuC2I	CCSGG	5 cut(s)	266, 267, 926, 1113, 1383
AsuHPI	GGTGA	6 cut(s)	67, 281, 587, 1106, 1541, 1930
AsuII	TTCGAA	2 cut(s)	901, 2339
AvaI	CYCGRG	2 cut(s)	265, 941
AxyI	CCTNAGG	2 cut(s)	396, 1878
BaeGI	GKGCMC	3 cut(s)	165, 1364, 1414
BalI	TGGCCA	1 cut(s)	1003
BanI	GGYRCC	1 cut(s)	1825
BarI	GAAGNNNNNNTAC	2 cut(s)	803, 835
BbrPI	CACGTG	1 cut(s)	1205
BbsI	GAAGAC	2 cut(s)	818, 890
Bbv12I	GWGCWC	2 cut(s)	1144, 1905
BbvI	GCAGC	8 cut(s)	50, 213, 301, 315, 944, 1403, 1708, 1993
BccI	CCATC	6 cut(s)	302, 479, 539, 896, 1351, 2178
BceAI	ACGGC	1 cut(s)	28
BcgI	CGANNNNNNTGC	6 cut(s)	193, 227, 296, 330, 2293, 2327
BciT130I	CCWGG	4 cut(s)	247, 1189, 1482, 1726
BciVI	GTATCC	1 cut(s)	1600
BclI	TGATCA	1 cut(s)	805
BcnI	CCSGG	5 cut(s)	266, 267, 926, 1113, 1383
BfaI	CTAG	2 cut(s)	24, 54
BfmI	CTRYAG	3 cut(s)	135, 1417, 2211
BfrI	CTTAAG	1 cut(s)	2186
BfuAI	ACCTGC	1 cut(s)	1879
BfuI	GTATCC	1 cut(s)	1600
BlpI	GCTNAGC	1 cut(s)	792
Bme1390I	CCNGG	9 cut(s)	247, 266, 267, 926, 1113, 1189, 1383, 1482, 1726
BmeT110I	CYCGRG	2 cut(s)	265, 941
BmgT120I	GGNCC	4 cut(s)	851, 949, 1285, 2385
BmiI	GGNNCC	4 cut(s)	951, 1287, 1425, 1827
BmrFI	CCNGG	9 cut(s)	247, 266, 267, 926, 1113, 1189, 1383, 1482, 1726
BmsI	GCATC	4 cut(s)	1078, 1214, 1758, 2301
BpiI	GAAGAC	2 cut(s)	818, 890
BpmI	CTGGAG	3 cut(s)	1494, 2046, 2312
Bpu10I	CCTNAGC	1 cut(s)	318
Bpu1102I	GCTNAGC	1 cut(s)	792
Bpu14I	TTCGAA	2 cut(s)	901, 2339
BpuEI	CTTGAG	3 cut(s)	173, 1293, 2390
BpuMI	CCSGG	5 cut(s)	266, 267, 926, 1113, 1383
BsaAI	YACGTR	1 cut(s)	1205
BsaHI	GRCGYC	2 cut(s)	216, 988
BsaI	GGTCTC	2 cut(s)	1471, 2053
BsaJI	CCNNGG	8 cut(s)	165, 265, 630, 942, 1188, 1381, 1427, 2141
BsaWI	WCCGGW	2 cut(s)	572, 1091
Bsc4I	CCNNNNNNNGG	8 cut(s)	246, 849, 1118, 1382, 1419, 1420, 2142, 2382
Bse118I	RCCGGY	2 cut(s)	1091, 1807
Bse1I	ACTGG	7 cut(s)	816, 830, 1511, 1593, 1838, 1910, 1958
Bse21I	CCTNAGG	2 cut(s)	396, 1878
Bse3DI	GCAATG	1 cut(s)	1281
BseBI	CCWGG	4 cut(s)	247, 1189, 1482, 1726
BseDI	CCNNGG	8 cut(s)	165, 265, 630, 942, 1188, 1381, 1427, 2141
BseGI	GGATG	6 cut(s)	123, 163, 601, 861, 1229, 1749
BseLI	CCNNNNNNNGG	8 cut(s)	246, 849, 1118, 1382, 1419, 1420, 2142, 2382
BseMI	GCAATG	1 cut(s)	1281
BseMII	CTCAG	9 cut(s)	332, 351, 387, 398, 411, 722, 773, 806, 1869
BseNI	ACTGG	7 cut(s)	816, 830, 1511, 1593, 1838, 1910, 1958
BseRI	GAGGAG	9 cut(s)	118, 121, 185, 377, 413, 437, 461, 485, 959
BseSI	GKGCMC	3 cut(s)	165, 1364, 1414
BseXI	GCAGC	8 cut(s)	50, 213, 301, 315, 944, 1403, 1708, 1993
BseYI	CCCAGC	4 cut(s)	848, 953, 1525, 1581
BsgI	GTGCAG	2 cut(s)	1334, 2387
BshNI	GGYRCC	1 cut(s)	1825
BshTI	ACCGGT	1 cut(s)	1091
BsiHKAI	GWGCWC	2 cut(s)	1144, 1905
BsiHKCI	CYCGRG	2 cut(s)	265, 941
BsiSI	CCGG	7 cut(s)	266, 573, 925, 1092, 1113, 1383, 1808
BslFI	GGGAC	1 cut(s)	924
BslI	CCNNNNNNNGG	8 cut(s)	246, 849, 1118, 1382, 1419, 1420, 2142, 2382
BsmBI	CGTCTC	2 cut(s)	347, 2259
BsmFI	GGGAC	1 cut(s)	924
BsmI	GAATGC	1 cut(s)	1555
Bso31I	GGTCTC	2 cut(s)	1471, 2053
BsoBI	CYCGRG	2 cut(s)	265, 941
Bsp119I	TTCGAA	2 cut(s)	901, 2339
Bsp1286I	GDGCHC	5 cut(s)	165, 1144, 1364, 1414, 1905
Bsp143I	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
Bsp1720I	GCTNAGC	1 cut(s)	792
BspACI	CCGC	7 cut(s)	51, 549, 1227, 1414, 2010, 2219, 2383
BspCNI	CTCAG	9 cut(s)	331, 352, 388, 397, 412, 721, 772, 805, 1870
BspHI	TCATGA	2 cut(s)	2037, 2110
BspLI	GGNNCC	4 cut(s)	951, 1287, 1425, 1827
BspMAI	CTGCAG	1 cut(s)	1421
BspMI	ACCTGC	1 cut(s)	1879
BspPI	GGATC	1 cut(s)	1706
BspQI	GCTCTTC	1 cut(s)	1149
BspT104I	TTCGAA	2 cut(s)	901, 2339
BspT107I	GGYRCC	1 cut(s)	1825
BspTI	CTTAAG	1 cut(s)	2186
BspTNI	GGTCTC	2 cut(s)	1471, 2053
BsrDI	GCAATG	1 cut(s)	1281
BsrFI	RCCGGY	2 cut(s)	1091, 1807
BsrI	ACTGG	7 cut(s)	816, 830, 1511, 1593, 1838, 1910, 1958
BssAI	RCCGGY	2 cut(s)	1091, 1807
BssECI	CCNNGG	8 cut(s)	165, 265, 630, 942, 1188, 1381, 1427, 2141
BssMI	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
BssNI	GRCGYC	2 cut(s)	216, 988
BssT1I	CCWWGG	3 cut(s)	630, 1427, 2141
Bst2UI	CCWGG	4 cut(s)	247, 1189, 1482, 1726
Bst4CI	ACNGT	6 cut(s)	73, 79, 605, 1170, 1409, 1612
Bst6I	CTCTTC	4 cut(s)	345, 975, 1149, 2186
BstACI	GRCGYC	2 cut(s)	216, 988
BstAFI	CTTAAG	1 cut(s)	2186
BstBAI	YACGTR	1 cut(s)	1205
BstBI	TTCGAA	2 cut(s)	901, 2339
BstC8I	GCNNGC	6 cut(s)	666, 798, 885, 1237, 1414, 1809
BstDSI	CCRYGG	1 cut(s)	165
BstENI	CCTNNNNNAGG	1 cut(s)	2140
BstF5I	GGATG	6 cut(s)	123, 163, 601, 861, 1229, 1749
BstKTI	GATC	5 cut(s)	808, 1676, 1714, 2155, 2230
BstMBI	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
BstMWI	GCNNNNNNNGC	7 cut(s)	549, 797, 1245, 1349, 2216, 2260, 2365
BstNI	CCWGG	4 cut(s)	247, 1189, 1482, 1726
BstSCI	CCNGG	9 cut(s)	245, 264, 265, 924, 1111, 1187, 1381, 1480, 1724
BstSFI	CTRYAG	3 cut(s)	135, 1417, 2211
BstSLI	GKGCMC	3 cut(s)	165, 1364, 1414
BstV1I	GCAGC	8 cut(s)	50, 213, 301, 315, 944, 1403, 1708, 1993
BstV2I	GAAGAC	2 cut(s)	818, 890
BstXI	CCANNNNNNTGG	1 cut(s)	999
Bsu36I	CCTNAGG	2 cut(s)	396, 1878
BsuI	GTATCC	1 cut(s)	1600
BtgI	CCRYGG	1 cut(s)	165
BtgZI	GCGATG	2 cut(s)	321, 558
BtsCI	GGATG	6 cut(s)	123, 163, 601, 861, 1229, 1749
BtsI	GCAGTG	2 cut(s)	843, 2321
BtsIMutI	CAGTG	6 cut(s)	610, 843, 1405, 1518, 1903, 2321
BveI	ACCTGC	1 cut(s)	1879
Cac8I	GCNNGC	6 cut(s)	666, 798, 885, 1237, 1414, 1809
CciI	TCATGA	2 cut(s)	2037, 2110
Cfr10I	RCCGGY	2 cut(s)	1091, 1807
Cfr13I	GGNCC	4 cut(s)	851, 949, 1285, 2385
Cfr9I	CCCGGG	1 cut(s)	265
CseI	GACGC	3 cut(s)	205, 293, 996
CsiI	ACCWGGT	1 cut(s)	1480
Csp6I	GTAC	3 cut(s)	906, 1027, 1993
CspAI	ACCGGT	1 cut(s)	1091
CviQI	GTAC	3 cut(s)	906, 1027, 1993
DpnI	GATC	5 cut(s)	807, 1675, 1713, 2154, 2229
DpnII	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
DraIII	CACNNNGTG	1 cut(s)	1759
DrdI	GACNNNNNNGTC	1 cut(s)	1265
DseDI	GACNNNNNNGTC	1 cut(s)	1265
EaeI	YGGCCR	3 cut(s)	1001, 1384, 1805
Eam1104I	CTCTTC	4 cut(s)	345, 975, 1149, 2186
EarI	CTCTTC	4 cut(s)	345, 975, 1149, 2186
Eco130I	CCWWGG	3 cut(s)	630, 1427, 2141
Eco31I	GGTCTC	2 cut(s)	1471, 2053
Eco57I	CTGAAG	2 cut(s)	501, 1875
Eco72I	CACGTG	1 cut(s)	1205
Eco81I	CCTNAGG	2 cut(s)	396, 1878
Eco88I	CYCGRG	2 cut(s)	265, 941
EcoNI	CCTNNNNNAGG	1 cut(s)	2140
EcoO109I	RGGNCCY	1 cut(s)	949
EcoRI	GAATTC	2 cut(s)	517, 1335
EcoRII	CCWGG	4 cut(s)	245, 1187, 1480, 1724
EcoT14I	CCWWGG	3 cut(s)	630, 1427, 2141
EcoT22I	ATGCAT	1 cut(s)	1751
ErhI	CCWWGG	3 cut(s)	630, 1427, 2141
Esp3I	CGTCTC	2 cut(s)	347, 2259
FaqI	GGGAC	1 cut(s)	924
FauI	CCCGC	1 cut(s)	1421
FbaI	TGATCA	1 cut(s)	805
FblI	GTMKAC	1 cut(s)	2055
FokI	GGATG	6 cut(s)	130, 170, 608, 848, 1236, 1736
FspBI	CTAG	2 cut(s)	24, 54
GsaI	CCCAGC	4 cut(s)	852, 957, 1529, 1585
GsuI	CTGGAG	3 cut(s)	1494, 2046, 2312
HapII	CCGG	7 cut(s)	266, 573, 925, 1092, 1113, 1383, 1808
HgaI	GACGC	3 cut(s)	205, 293, 996
Hin1I	GRCGYC	2 cut(s)	216, 988
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HindIII	AAGCTT	5 cut(s)	1049, 1237, 1448, 2183, 2252
HpaII	CCGG	7 cut(s)	266, 573, 925, 1092, 1113, 1383, 1808
HphI	GGTGA	6 cut(s)	67, 281, 587, 1106, 1541, 1930
Hpy166II	GTNNAC	6 cut(s)	185, 756, 1202, 1405, 1993, 2056
Hpy188III	TCNNGA	9 cut(s)	386, 892, 941, 974, 1178, 1877, 2038, 2063, 2111
Hpy8I	GTNNAC	6 cut(s)	185, 756, 1202, 1405, 1993, 2056
Hpy99I	CGWCG	3 cut(s)	215, 284, 287
HpyAV	CCTTC	8 cut(s)	708, 778, 971, 1387, 1433, 1552, 2246, 2335
HpyCH4III	ACNGT	6 cut(s)	73, 79, 605, 1170, 1409, 1612
HpyCH4IV	ACGT	6 cut(s)	210, 1198, 1204, 1780, 1912, 2333
HpyF10VI	GCNNNNNNNGC	7 cut(s)	549, 797, 1245, 1349, 2216, 2260, 2365
HpySE526I	ACGT	6 cut(s)	210, 1198, 1204, 1780, 1912, 2333
Hsp92I	GRCGYC	2 cut(s)	216, 988
KroI	GCCGGC	1 cut(s)	1807
KroNI	GCCGGC	1 cut(s)	1809
Ksp22I	TGATCA	1 cut(s)	805
Kzo9I	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
LguI	GCTCTTC	1 cut(s)	1149
LmnI	GCTCC	5 cut(s)	71, 193, 715, 1459, 1879
Lsp1109I	GCAGC	8 cut(s)	50, 213, 301, 315, 944, 1403, 1708, 1993
LweI	GCATC	4 cut(s)	1078, 1214, 1758, 2301
MabI	ACCWGGT	1 cut(s)	1480
MaeI	CTAG	2 cut(s)	24, 54
MaeII	ACGT	6 cut(s)	210, 1198, 1204, 1780, 1912, 2333
MaeIII	GTNAC	7 cut(s)	73, 233, 269, 619, 818, 1087, 1918
MalI	GATC	5 cut(s)	807, 1675, 1713, 2154, 2229
MboI	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
MfeI	CAATTG	2 cut(s)	993, 2415
MhlI	GDGCHC	5 cut(s)	165, 1144, 1364, 1414, 1905
MlsI	TGGCCA	1 cut(s)	1003
MluNI	TGGCCA	1 cut(s)	1003
MlyI	GAGTC	8 cut(s)	376, 485, 644, 779, 904, 2051, 2132, 2402
MmeI	TCCRAC	5 cut(s)	693, 1201, 1437, 1797, 2376
Mox20I	TGGCCA	1 cut(s)	1003
Mph1103I	ATGCAT	1 cut(s)	1751
MroNI	GCCGGC	1 cut(s)	1807
MroXI	GAANNNNTTC	1 cut(s)	1198
MscI	TGGCCA	1 cut(s)	1003
MseI	TTAA	7 cut(s)	1062, 1767, 1841, 1950, 2130, 2187, 2422
MslI	CAYNNNNRTG	1 cut(s)	2042
Msp20I	TGGCCA	1 cut(s)	1003
MspA1I	CMGCKG	2 cut(s)	957, 1416
MspCI	CTTAAG	1 cut(s)	2186
MspI	CCGG	7 cut(s)	266, 573, 925, 1092, 1113, 1383, 1808
MspR9I	CCNGG	9 cut(s)	247, 266, 267, 926, 1113, 1189, 1383, 1482, 1726
MunI	CAATTG	2 cut(s)	993, 2415
Mva1269I	GAATGC	1 cut(s)	1555
MvaI	CCWGG	4 cut(s)	247, 1189, 1482, 1726
MwoI	GCNNNNNNNGC	7 cut(s)	549, 797, 1245, 1349, 2216, 2260, 2365
NaeI	GCCGGC	1 cut(s)	1809
NciI	CCSGG	5 cut(s)	266, 267, 926, 1113, 1383
NdeII	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
NgoMIV	GCCGGC	1 cut(s)	1807
NlaIV	GGNNCC	4 cut(s)	951, 1287, 1425, 1827
NmuCI	GTSAC	4 cut(s)	73, 233, 269, 1918
NsiI	ATGCAT	1 cut(s)	1751
NspV	TTCGAA	2 cut(s)	901, 2339
PagI	TCATGA	2 cut(s)	2037, 2110
PciSI	GCTCTTC	1 cut(s)	1149
PcsI	WCGNNNNNNNCGW	2 cut(s)	2236, 2265
PctI	GAATGC	1 cut(s)	1555
PdiI	GCCGGC	1 cut(s)	1809
PdmI	GAANNNNTTC	1 cut(s)	1198
PfeI	GAWTC	2 cut(s)	569, 2232
PflFI	GACNNNGTC	1 cut(s)	1924
PinAI	ACCGGT	1 cut(s)	1091
PleI	GAGTC	8 cut(s)	376, 485, 643, 778, 903, 2051, 2131, 2401
PmaCI	CACGTG	1 cut(s)	1205
PmlI	CACGTG	1 cut(s)	1205
PpsI	GAGTC	8 cut(s)	376, 485, 643, 778, 903, 2051, 2131, 2401
Ppu21I	YACGTR	1 cut(s)	1205
Psp1406I	AACGTT	1 cut(s)	1198
Psp6I	CCWGG	4 cut(s)	245, 1187, 1480, 1724
PspCI	CACGTG	1 cut(s)	1205
PspFI	CCCAGC	4 cut(s)	848, 953, 1525, 1581
PspGI	CCWGG	4 cut(s)	245, 1187, 1480, 1724
PspN4I	GGNNCC	4 cut(s)	951, 1287, 1425, 1827
PspPI	GGNCC	4 cut(s)	851, 949, 1285, 2385
PstI	CTGCAG	1 cut(s)	1421
PsyI	GACNNNGTC	1 cut(s)	1924
PvuII	CAGCTG	1 cut(s)	957
RsaI	GTAC	3 cut(s)	907, 1028, 1994
RsaNI	GTAC	3 cut(s)	906, 1027, 1993
RseI	CAYNNNNRTG	1 cut(s)	2042
SapI	GCTCTTC	1 cut(s)	1149
SaqAI	TTAA	7 cut(s)	1062, 1767, 1841, 1950, 2130, 2187, 2422
Sau3AI	GATC	5 cut(s)	805, 1673, 1711, 2152, 2227
Sau96I	GGNCC	4 cut(s)	851, 949, 1285, 2385
SchI	GAGTC	8 cut(s)	376, 485, 644, 779, 904, 2051, 2132, 2402
ScrFI	CCNGG	9 cut(s)	247, 266, 267, 926, 1113, 1189, 1383, 1482, 1726
SduI	GDGCHC	5 cut(s)	165, 1144, 1364, 1414, 1905
SexAI	ACCWGGT	1 cut(s)	1480
SfaNI	GCATC	4 cut(s)	1078, 1214, 1758, 2301
SfcI	CTRYAG	3 cut(s)	135, 1417, 2211
SfuI	TTCGAA	2 cut(s)	901, 2339
SmaI	CCCGGG	1 cut(s)	267
SmiMI	CAYNNNNRTG	1 cut(s)	2042
SmlI	CTYRAG	4 cut(s)	188, 1308, 2186, 2405
SmoI	CTYRAG	4 cut(s)	188, 1308, 2186, 2405
SsiI	CCGC	7 cut(s)	51, 549, 1227, 1414, 2010, 2219, 2383
SspMI	CTAG	2 cut(s)	24, 54
StyD4I	CCNGG	9 cut(s)	245, 264, 265, 924, 1111, 1187, 1381, 1480, 1724
StyI	CCWWGG	3 cut(s)	630, 1427, 2141
TaaI	ACNGT	6 cut(s)	73, 79, 605, 1170, 1409, 1612
TaiI	ACGT	6 cut(s)	213, 1201, 1207, 1783, 1915, 2336
TaqI	TCGA	5 cut(s)	901, 2230, 2239, 2309, 2339
TauI	GCSGC	3 cut(s)	552, 2012, 2386
TfiI	GAWTC	2 cut(s)	569, 2232
Tru1I	TTAA	7 cut(s)	1062, 1767, 1841, 1950, 2130, 2187, 2422
Tru9I	TTAA	7 cut(s)	1062, 1767, 1841, 1950, 2130, 2187, 2422
TscAI	CASTG	6 cut(s)	610, 850, 1412, 1518, 1910, 2321
TseFI	GTSAC	4 cut(s)	73, 233, 269, 1918
TseI	GCWGC	8 cut(s)	63, 201, 303, 314, 957, 1416, 1721, 2006
Tsp45I	GTSAC	4 cut(s)	73, 233, 269, 1918
TspDTI	ATGAA	6 cut(s)	850, 1136, 1150, 1317, 1571, 2054
TspGWI	ACGGA	3 cut(s)	182, 691, 2010
TspMI	CCCGGG	1 cut(s)	265
TspRI	CASTG	6 cut(s)	610, 850, 1412, 1518, 1910, 2321
Tth111I	GACNNNGTC	1 cut(s)	1924
Vha464I	CTTAAG	1 cut(s)	2186
XagI	CCTNNNNNAGG	1 cut(s)	2140
XapI	RAATTY	3 cut(s)	517, 1335, 2028
XcmI	CCANNNNNNNNNTGG	2 cut(s)	151, 1722
XmaI	CCCGGG	1 cut(s)	265
XmiI	GTMKAC	1 cut(s)	2055
XmnI	GAANNNNTTC	1 cut(s)	1198
XspI	CTAG	2 cut(s)	24, 54
Zsp2I	ATGCAT	1 cut(s)	1751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.