FvH4_1g25280

Rieske-like [2Fe-2S] domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014101
Physical Location & Seq
Forward (+)
17071672 .. 17073888
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25280.t1

Sequence Viewer

Length: 855 bp
ATGGCCAACGCCTCACCCTTCGCCCCCTCATTTCCCCGTAATCACCTCCTTACCCCTCCCTCTTCCCGCCCTCTCCTCCCCAAACCGCCCCTCACTCTTTCTACTCATCCCATTTCATCATCATCATCCTCATCATCCCTCCATGCACTAGGGAAGAATTACCATTCTTTCCCTCGACGACCAACCAAGGCGACGGAGGTCTCGGTTGCGGAAACGGAGAACTGGGTGCCGGTGGTGCCGCTGGCGGCGCTTCCCAAAGGAGAGCGGCGCGTGATAGTTCAGGACGGAGAGGCCATACTGCTGCTGTGGTACAAGGACCAAGTCTTCGCCATTGAGAATCGCTCGCCGGCGGAAGGTGCCTACAGTGAAGGCCTCCTCAATGCCAAGCTCACTCAGGACGGGTGTATTGTGTGTCCATCAACTGATAGCACATTTGATCTTCGGAGTGGCGCCGTCAAGGATTGGTACCCGAAAAACCCGGTGCTGAGAGCTCTCACACCGGCTTTGAGGACTCTTTATATTTATCCTTGCAAGACTGATGAGAAGAACATCTACATTAGCCTCGAAGACGGAACAATAAATTCTGATGCAGCTGCCGAGATTGTGTTCAGCGGCAAGGCTCAACCTGGTGTCACTGCTTCTGATGTTAATATCGAGGAGGTGAAAATGGTGGTTGATGAGAGCTTAGAAGGAGGGGCGTTTGGTTTCACAAGGAGGAATGAAATAATAAATGGGAAGGCAGCTACGATTGGGTTCCTCTTGTTATTAGATATTGAGCTCTTGACTGGTAAAGGACTTCTTAAGGGAACTGGCTTCTTGGACTTTATTTATTCTGTTTCAAATGCTCTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rieske_2

Protein Analysis

285

Amino Acids

30.73

Weight (kDa)

5.87

Isoelectric Point (pI)

57.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rieske_2 PF13806 74 - 186 2e-19 Rieske-like [2Fe-2S] domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71500
fragaria_vesca FvH4_1g25280 FvH4_1g25280
malus_domestica MD15G1137600.v1.1
prunus_persica Prupe.1G128000_v2.0.a1
pyrus_communis pycom15g12400
rosa_chinensis RchiOBHm_Chr2g0123251
rosa_laevigata RLG00000018744
rosa_multiflora Rmu_sc0011914.1_g000003
rosa_roxburghii Rroxscaffold_2G00120820
rosa_rugosa Rorug02G0244200
rosa_samantha Rh2AG303400 Rh2BG311300 Rh2CG290000 Rh2DG327400
rosa_wichuraiana Rw0G013110 Rw2G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 5 cut(s) 226, 235, 356, 449, 465
AccBSI CCGCTC 1 cut(s) 265
AccII CGCG 1 cut(s) 270
AciI CCGC 8 cut(s) 67, 86, 209, 239, 245, 265, 350, 612
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 2 cut(s) 311, 467
AfiI CCNNNNNNNGG 1 cut(s) 353
AflII CTTAAG 1 cut(s) 800
AgsI TTSAA 1 cut(s) 840
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 6 cut(s) 388, 491, 593, 684, 743, 778
AluI AGCT 6 cut(s) 388, 491, 593, 684, 743, 778
Alw21I GWGCWC 2 cut(s) 493, 780
Alw26I GTCTC 1 cut(s) 205
AoxI GGCC 3 cut(s) 3, 291, 370
ApeKI GCWGC 4 cut(s) 301, 590, 593, 740
ApoI RAATTY 1 cut(s) 580
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
Asp718I GGTACC 1 cut(s) 465
AspLEI GCGC 3 cut(s) 250, 270, 452
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 6, 35, 673
AvaII GGWCC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 5 cut(s) 226, 235, 356, 449, 465
BanII GRGCYC 2 cut(s) 493, 780
BarI GAAGNNNNNNTAC 2 cut(s) 536, 568
BbsI GAAGAC 2 cut(s) 316, 573
Bbv12I GWGCWC 2 cut(s) 493, 780
BbvI GCAGC 4 cut(s) 288, 580, 602, 752
BccI CCATC 1 cut(s) 424
BceAI ACGGC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 361
BfoI RGCGCY 2 cut(s) 251, 453
BfrI CTTAAG 1 cut(s) 800
BisI GCNGC 8 cut(s) 239, 246, 266, 302, 591, 594, 613, 741
BlsI GCNGC 8 cut(s) 240, 247, 267, 303, 592, 595, 614, 742
Bme1390I CCNGG 2 cut(s) 479, 627
Bme18I GGWCC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 6 cut(s) 228, 237, 358, 451, 467, 755
BmrFI CCNGG 2 cut(s) 479, 627
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 577
BmuI ACTGGG 1 cut(s) 232
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 2 cut(s) 316, 573
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 450
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse118I RCCGGY 3 cut(s) 229, 346, 499
Bse1I ACTGG 4 cut(s) 227, 790, 814, 850
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 3 cut(s) 106, 125, 134
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 407, 476
BseNI ACTGG 4 cut(s) 227, 790, 814, 850
BseRI GAGGAG 3 cut(s) 65, 365, 671
BseXI GCAGC 4 cut(s) 288, 580, 602, 752
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 5, 293, 372
BshNI GGYRCC 5 cut(s) 226, 235, 356, 449, 465
BsiHKAI GWGCWC 2 cut(s) 493, 780
BsiSI CCGG 4 cut(s) 230, 347, 479, 500
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 205
BsnI GGCC 3 cut(s) 5, 293, 372
Bso31I GGTCTC 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 493, 780
Bsp143I GATC 1 cut(s) 436
BspACI CCGC 8 cut(s) 67, 86, 209, 239, 245, 265, 350, 612
BspANI GGCC 3 cut(s) 5, 293, 372
BspCNI CTCAG 2 cut(s) 406, 477
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 6 cut(s) 228, 237, 358, 451, 467, 755
BspT107I GGYRCC 5 cut(s) 226, 235, 356, 449, 465
BspTI CTTAAG 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 265
BsrFI RCCGGY 3 cut(s) 229, 346, 499
BsrI ACTGG 4 cut(s) 227, 790, 814, 850
BssAI RCCGGY 3 cut(s) 229, 346, 499
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 436
BssNI GRCGYC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 365
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 800
BstC8I GCNNGC 3 cut(s) 243, 344, 348
BstDEI CTNAG 3 cut(s) 393, 485, 685
BstF5I GGATG 3 cut(s) 106, 125, 134
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 2 cut(s) 251, 453
BstHHI GCGC 3 cut(s) 250, 270, 452
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 1 cut(s) 436
BstMWI GCNNNNNNNGC 3 cut(s) 235, 247, 356
BstNI CCWGG 1 cut(s) 627
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 477, 625
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 4 cut(s) 288, 580, 602, 752
BstV2I GAAGAC 2 cut(s) 316, 573
BsuRI GGCC 3 cut(s) 5, 293, 372
BtsCI GGATG 3 cut(s) 106, 125, 134
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 370, 633
Cac8I GCNNGC 3 cut(s) 243, 344, 348
CfoI GCGC 3 cut(s) 250, 270, 452
Cfr10I RCCGGY 3 cut(s) 229, 346, 499
Cfr13I GGNCC 1 cut(s) 316
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 310, 466
CviAII CATG 1 cut(s) 143
CviQI GTAC 2 cut(s) 310, 466
DdeI CTNAG 3 cut(s) 393, 485, 685
DinI GGCGCC 1 cut(s) 451
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 365
Ecl136II GAGCTC 2 cut(s) 491, 778
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 493, 780
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 2 cut(s) 491, 778
EcoICRI GAGCTC 2 cut(s) 491, 778
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 493, 780
EgeI GGCGCC 1 cut(s) 451
EheI GGCGCC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 146
FaiI YATR 3 cut(s) 144, 296, 519
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 1 cut(s) 142
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 8 cut(s) 239, 246, 266, 302, 591, 594, 613, 741
FokI GGATG 3 cut(s) 93, 112, 121
FriOI GRGCYC 2 cut(s) 493, 780
Fsp4HI GCNGC 8 cut(s) 239, 246, 266, 302, 591, 594, 613, 741
FspBI CTAG 1 cut(s) 149
GlaI GCGC 3 cut(s) 249, 269, 451
GluI GCNGC 8 cut(s) 239, 246, 266, 302, 591, 594, 613, 741
GsuI CTGGAG 1 cut(s) 833
HaeII RGCGCY 2 cut(s) 251, 453
HaeIII GGCC 3 cut(s) 5, 293, 372
HapII CCGG 4 cut(s) 230, 347, 479, 500
HhaI GCGC 3 cut(s) 250, 270, 452
Hin1I GRCGYC 1 cut(s) 450
Hin1II CATG 1 cut(s) 146
Hin6I GCGC 3 cut(s) 248, 268, 450
HinP1I GCGC 3 cut(s) 248, 268, 450
HinfI GANTC 2 cut(s) 337, 511
HpaII CCGG 4 cut(s) 230, 347, 479, 500
HphI GGTGA 3 cut(s) 6, 35, 673
Hpy188I TCNGA 3 cut(s) 444, 586, 643
Hpy188III TCNNGA 3 cut(s) 281, 395, 781
Hpy99I CGWCG 2 cut(s) 180, 196
HpyAV CCTTC 5 cut(s) 28, 347, 362, 683, 730
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4V TGCA 3 cut(s) 146, 531, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 247, 356
HpyF3I CTNAG 3 cut(s) 393, 485, 685
Hsp92I GRCGYC 1 cut(s) 450
Hsp92II CATG 1 cut(s) 146
HspAI GCGC 3 cut(s) 248, 268, 450
KasI GGCGCC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 469
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Kzo9I GATC 1 cut(s) 436
Lsp1109I GCAGC 4 cut(s) 288, 580, 602, 752
LweI GCATC 1 cut(s) 577
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 631
MalI GATC 1 cut(s) 438
MbiI CCGCTC 1 cut(s) 265
MboI GATC 1 cut(s) 436
MboII GAAGA 6 cut(s) 54, 166, 316, 431, 556, 578
MhlI GDGCHC 2 cut(s) 493, 780
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 157, 580
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 450
MlyI GAGTC 1 cut(s) 505
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 346
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 648, 801
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 241, 593, 612
MspCI CTTAAG 1 cut(s) 800
MspI CCGG 4 cut(s) 230, 347, 479, 500
MspR9I CCNGG 2 cut(s) 479, 627
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 235, 247, 356
NaeI GCCGGC 1 cut(s) 348
NarI GGCGCC 1 cut(s) 450
NciI CCSGG 1 cut(s) 479
NdeII GATC 1 cut(s) 436
NgoMIV GCCGGC 1 cut(s) 346
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 6 cut(s) 228, 237, 358, 451, 467, 755
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 631
PceI AGGCCT 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 337
PflFI GACNNNGTC 1 cut(s) 320
PkrI GCNGC 8 cut(s) 240, 247, 267, 303, 592, 595, 614, 742
PleI GAGTC 1 cut(s) 505
PluTI GGCGCC 1 cut(s) 453
PpsI GAGTC 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 197
Psp124BI GAGCTC 2 cut(s) 493, 780
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 6 cut(s) 228, 237, 358, 451, 467, 755
PspPI GGNCC 1 cut(s) 316
PsyI GACNNNGTC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 2 cut(s) 311, 467
RsaNI GTAC 2 cut(s) 310, 466
SacI GAGCTC 2 cut(s) 493, 780
SaqAI TTAA 2 cut(s) 648, 801
SatI GCNGC 8 cut(s) 239, 246, 266, 302, 591, 594, 613, 741
Sau3AI GATC 1 cut(s) 436
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 2 cut(s) 479, 627
SduI GDGCHC 2 cut(s) 493, 780
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 361
SfoI GGCGCC 1 cut(s) 451
SgrAI CRCCGGYG 1 cut(s) 346
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 2 cut(s) 157, 580
SseBI AGGCCT 1 cut(s) 372
SsiI CCGC 8 cut(s) 67, 86, 209, 239, 245, 265, 350, 612
SspDI GGCGCC 1 cut(s) 449
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 2 cut(s) 493, 780
StuI AGGCCT 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 477, 625
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 365
TaqI TCGA 3 cut(s) 175, 564, 654
TasI AATT 2 cut(s) 157, 580
TauI GCSGC 4 cut(s) 241, 248, 268, 615
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 2 cut(s) 648, 801
Tru9I TTAA 2 cut(s) 648, 801
TscAI CASTG 2 cut(s) 370, 640
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 4 cut(s) 301, 590, 593, 740
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 2 cut(s) 105, 735
TspGWI ACGGA 4 cut(s) 209, 230, 300, 585
TspRI CASTG 2 cut(s) 370, 640
Tth111I GACNNNGTC 1 cut(s) 320
Vha464I CTTAAG 1 cut(s) 800
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 580
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.