FvH4_1g28230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009971
Physical Location & Seq
Reverse (-)
20065272 .. 20066268
997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28230.t1

Sequence Viewer

Length: 852 bp
ATGGATCTTGATGAGTGGGAATACCTCCCTGATGACGCTTTCCTTGATTTCCATGAACAAAGTGAGAAGAAGAAGGTCTTGTTTAGCAAGCGCTATTTCGATTCAACTAGTGTGTTCAATATGTACCACGACTACTACATAGTCCAATCACCCAATCCTTCCAGTTCCAAGAAACTAGCAGCCACCGATCAAGTAGCTCCTCCTGCTGTTGTGCCTCCTAATCAACTTGCTGTTCCTGTTCAGATTCAACTGCTTCCTCCACCAAGCTTTGTCAGAACCACACAAACACCAGATGGAGAAAAGCAACACACTCAAGACCCCCTACTCTCCCAAGTTCACTTCAAGAAAACTACTTGGGAACCCGAATCTGTCGACATGAAAATGGACTCACCAAAGTCCCCTACCAGAGGAGGATTAGGTTTCGGATTTGTGCATCAAACTACTAGTGATTCTACTGCCGCTGCTGGAGGAGCCAAGTTTGAGTTTGATGATTATAATGGTGAGCTCGCCAAGGTTAGCAGCTCTCCAAGAATGAAAACCGACCAAGAGGAGGAGGAGGACGAGGAACAAGGAATAAAGAGGCAGAAGAATAACATCTGGAAGTTGAGCTTCACCGGAATCGGAGCTATTTGCTCTTTTGGTTTTGCTGCAGCTACCATTTGTGTTCTGTTTTTGGGTAGCCACCACCAAAGTAACCACAAACACTTGCAGATCTACACTGATGACACCAAGAGGATTAAGGAGGTGGTTCACCGAGCAACCAAGTTTGACGAAGCATTTTCAGCAGTGAGAGGGGTACCTCTAAGCAGAGCTCATATAACATCGGGAGGTTACTATACTAGTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.68

Weight (kDa)

5.88

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 106 - 281 1.2e-47 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 796
AccB1I GGYRCC 1 cut(s) 796
AccI GTMKAC 1 cut(s) 372
AciI CCGC 1 cut(s) 459
AclWI GGATC 1 cut(s) 12
AfaI GTAC 2 cut(s) 125, 798
AfeI AGCGCT 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 407, 550
AgsI TTSAA 4 cut(s) 105, 118, 248, 343
AhlI ACTAGT 3 cut(s) 107, 443, 839
AluBI AGCT 8 cut(s) 197, 267, 505, 522, 609, 626, 653, 812
AluI AGCT 8 cut(s) 197, 267, 505, 522, 609, 626, 653, 812
Alw21I GWGCWC 3 cut(s) 507, 814, 847
AlwI GGATC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 92
ApeKI GCWGC 5 cut(s) 179, 461, 519, 647, 650
Asp718I GGTACC 1 cut(s) 796
AspLEI GCGC 1 cut(s) 93
AsuHPI GGTGA 5 cut(s) 141, 381, 512, 604, 743
BanI GGYRCC 1 cut(s) 796
BanII GRGCYC 2 cut(s) 507, 814
Bbv12I GWGCWC 3 cut(s) 507, 814, 847
BbvI GCAGC 5 cut(s) 191, 448, 531, 634, 662
BccI CCATC 1 cut(s) 287
BcuI ACTAGT 3 cut(s) 107, 443, 839
BfaI CTAG 4 cut(s) 108, 176, 444, 840
BfmI CTRYAG 1 cut(s) 648
BfoI RGCGCY 1 cut(s) 94
BglII AGATCT 1 cut(s) 711
BisI GCNGC 6 cut(s) 180, 459, 462, 520, 648, 651
BlsI GCNGC 6 cut(s) 181, 460, 463, 521, 649, 652
BmiI GGNNCC 3 cut(s) 360, 472, 798
BmsI GCATC 1 cut(s) 442
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 550
Bse1I ACTGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 407, 550
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 6 cut(s) 189, 423, 483, 563, 566, 569
BseXI GCAGC 5 cut(s) 191, 448, 531, 634, 662
BshNI GGYRCC 1 cut(s) 796
BsiHKAI GWGCWC 3 cut(s) 507, 814, 847
BsiSI CCGG 1 cut(s) 615
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 2 cut(s) 407, 550
BsmFI GGGAC 1 cut(s) 382
Bsp1286I GDGCHC 3 cut(s) 507, 814, 847
Bsp143I GATC 3 cut(s) 4, 187, 711
BspACI CCGC 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 360, 472, 798
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 796
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 3 cut(s) 4, 187, 711
BssT1I CCWWGG 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 89, 507
BstDEI CTNAG 1 cut(s) 803
BstENI CCTNNNNNAGG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 3 cut(s) 7, 190, 714
BstMBI GATC 3 cut(s) 4, 187, 711
BstMWI GCNNNNNNNGC 3 cut(s) 203, 470, 782
BstSFI CTRYAG 1 cut(s) 648
BstV1I GCAGC 5 cut(s) 191, 448, 531, 634, 662
BstX2I RGATCY 2 cut(s) 4, 711
BstYI RGATCY 2 cut(s) 4, 711
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 2 cut(s) 717, 792
Cac8I GCNNGC 2 cut(s) 89, 507
CfoI GCGC 1 cut(s) 93
CseI GACGC 1 cut(s) 44
Csp6I GTAC 2 cut(s) 124, 797
CviAII CATG 2 cut(s) 53, 376
CviQI GTAC 2 cut(s) 124, 797
DdeI CTNAG 1 cut(s) 803
DpnI GATC 3 cut(s) 6, 189, 713
DpnII GATC 3 cut(s) 4, 187, 711
Ecl136II GAGCTC 2 cut(s) 505, 812
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 2 cut(s) 507, 814
Eco47III AGCGCT 1 cut(s) 92
Eco53kI GAGCTC 2 cut(s) 505, 812
EcoICRI GAGCTC 2 cut(s) 505, 812
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 2 cut(s) 507, 814
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 2 cut(s) 56, 379
FaiI YATR 9 cut(s) 54, 122, 140, 377, 495, 816, 818, 837, 850
FalI AAGNNNNNCTT 4 cut(s) 62, 94, 593, 625
FaqI GGGAC 1 cut(s) 382
FatI CATG 2 cut(s) 52, 375
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 6 cut(s) 180, 459, 462, 520, 648, 651
FriOI GRGCYC 2 cut(s) 507, 814
Fsp4HI GCNGC 6 cut(s) 180, 459, 462, 520, 648, 651
FspBI CTAG 4 cut(s) 108, 176, 444, 840
GlaI GCGC 1 cut(s) 92
GluI GCNGC 6 cut(s) 180, 459, 462, 520, 648, 651
GsuI CTGGAG 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 94
HapII CCGG 1 cut(s) 615
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 93
Hin1II CATG 2 cut(s) 56, 379
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 6 cut(s) 101, 244, 365, 386, 449, 618
HpaII CCGG 1 cut(s) 615
HphI GGTGA 5 cut(s) 141, 381, 512, 604, 743
Hpy166II GTNNAC 3 cut(s) 337, 373, 751
Hpy188I TCNGA 4 cut(s) 243, 275, 425, 623
Hpy188III TCNNGA 5 cut(s) 8, 314, 343, 598, 825
Hpy8I GTNNAC 3 cut(s) 337, 373, 751
HpyAV CCTTC 2 cut(s) 67, 168
HpyCH4V TGCA 3 cut(s) 433, 650, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 470, 782
HpyF3I CTNAG 1 cut(s) 803
Hsp92II CATG 2 cut(s) 56, 379
HspAI GCGC 1 cut(s) 91
KpnI GGTACC 1 cut(s) 800
Kzo9I GATC 3 cut(s) 4, 187, 711
LmnI GCTCC 3 cut(s) 202, 470, 623
LpnPI CCDG 9 cut(s) 42, 175, 216, 249, 303, 418, 450, 583, 628
Lsp1109I GCAGC 5 cut(s) 191, 448, 531, 634, 662
LweI GCATC 1 cut(s) 442
MaeI CTAG 4 cut(s) 108, 176, 444, 840
MaeIII GTNAC 2 cut(s) 692, 830
MalI GATC 3 cut(s) 6, 189, 713
MboI GATC 3 cut(s) 4, 187, 711
MboII GAAGA 3 cut(s) 79, 82, 598
MflI RGATCY 2 cut(s) 4, 711
MhlI GDGCHC 3 cut(s) 507, 814, 847
MlyI GAGTC 1 cut(s) 380
MseI TTAA 1 cut(s) 738
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 1 cut(s) 615
MwoI GCNNNNNNNGC 3 cut(s) 203, 470, 782
NdeII GATC 3 cut(s) 4, 187, 711
NlaIII CATG 2 cut(s) 56, 379
NlaIV GGNNCC 3 cut(s) 360, 472, 798
PfeI GAWTC 5 cut(s) 101, 244, 365, 449, 618
PkrI GCNGC 6 cut(s) 181, 460, 463, 521, 649, 652
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 507, 814
PspN4I GGNNCC 3 cut(s) 360, 472, 798
PstI CTGCAG 1 cut(s) 652
PsuI RGATCY 2 cut(s) 4, 711
RsaI GTAC 2 cut(s) 125, 798
RsaNI GTAC 2 cut(s) 124, 797
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 2 cut(s) 507, 814
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 738
SatI GCNGC 6 cut(s) 180, 459, 462, 520, 648, 651
Sau3AI GATC 3 cut(s) 4, 187, 711
SchI GAGTC 1 cut(s) 380
SduI GDGCHC 3 cut(s) 507, 814, 847
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SpeI ACTAGT 3 cut(s) 107, 443, 839
SsiI CCGC 1 cut(s) 459
SspMI CTAG 4 cut(s) 108, 176, 444, 840
SstI GAGCTC 2 cut(s) 507, 814
StyI CCWWGG 1 cut(s) 510
TaqI TCGA 2 cut(s) 99, 372
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 5 cut(s) 101, 244, 365, 449, 618
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 2 cut(s) 724, 792
TseI GCWGC 5 cut(s) 179, 461, 519, 647, 650
TspDTI ATGAA 3 cut(s) 69, 392, 548
TspRI CASTG 2 cut(s) 724, 792
XagI CCTNNNNNAGG 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 108, 176, 444, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.