pycom12g00420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009971
Physical Location & Seq
Forward (+)
415493 .. 416269
777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00420.1

Sequence Viewer

Length: 777 bp
ATGGATCTTGATGAGTGGGAGTACCTACCAGATGATGCGTTTCTTGATTTCCATGAAGACAGTGAAGAGAAGAGGATTTTTTCGAGCAAACGCTATTCTTCTTCTGATAATTCAAAAAGTGTTTTCAACATGAACTACTTCATAATCCCATCACCCAGTTCTAGGTCAAAAGTCATTCACGAGACACCACCACGACGACAACCGCCAACACCACCACCACCACCGGCGTCGCCAGCTTGGATTAATTCAAGTCTTGTTCCCGTTCCAATTCAGCTGGAACTACCTCCAAGCTTTGACAATGCCTCATCACTTTCACAAGAAGAACAAGAAGAAGAAAATCAAGTCATCAAAGTCTCCCAAGTGGATCAAGACGCACAACTCTCCCAAGTTTACTTCAAGAAAATTTGGGAAAATGAATTTGCCGACATGAAAATATTGGACTCCCCAAAGTCTCCCACCTGCAGATCACCCAAGTTTATGCATCAACAAACTGATAAGTTTCAGTTTGATGACAATTTAGGTCAAGAAATCACCTCTCCAAGGAAGGAAGTTGTTGACAAAAACAGCTTGTTGGAGTCATCAGAAGAGATAAATAGCAACAACAACATCTGGAAGCTGAGTTTTACAGGAATCGGAGCAATTTGCTCTTTTGGTTTTGCTGCTGCTACCGTTTGTATTATCTTTTTCGGTGCCCACCAGAGGAACAAACAGTATCAGCACAACCAGAATCGCTTCCAGATCTACACTGATGACAACAAGGTCAGCAAATTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.67

Weight (kDa)

4.96

Isoelectric Point (pI)

66.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 123 - 255 5.6e-36 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
AasI GACNNNNNNGTC 1 cut(s) 758
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 689
AciI CCGC 1 cut(s) 203
AclWI GGATC 2 cut(s) 12, 372
AcsI RAATTY 3 cut(s) 402, 416, 767
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 3 cut(s) 162, 540, 699
AgsI TTSAA 4 cut(s) 114, 127, 249, 397
AluBI AGCT 5 cut(s) 236, 274, 291, 567, 616
AluI AGCT 5 cut(s) 236, 274, 291, 567, 616
Alw26I GTCTC 3 cut(s) 176, 358, 456
AlwI GGATC 2 cut(s) 12, 372
ApeKI GCWGC 2 cut(s) 659, 662
ApoI RAATTY 3 cut(s) 402, 416, 767
AseI ATTAAT 1 cut(s) 243
Asp700I GAANNNNTTC 2 cut(s) 137, 731
AsuHPI GGTGA 3 cut(s) 144, 459, 523
BaeGI GKGCMC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 689
BauI CACGAG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 646, 649
BccI CCATC 1 cut(s) 157
BcoDI GTCTC 3 cut(s) 176, 358, 456
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 738
BisI GCNGC 2 cut(s) 660, 663
BlsI GCNGC 2 cut(s) 661, 664
BmiI GGNNCC 1 cut(s) 691
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 25, 490
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 539
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 540, 699
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 539
BseLI CCNNNNNNNGG 3 cut(s) 162, 540, 699
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 2 cut(s) 646, 649
BshNI GGYRCC 1 cut(s) 689
BsiSI CCGG 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 162, 540, 699
BsmAI GTCTC 3 cut(s) 176, 358, 456
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 4 cut(s) 4, 364, 464, 738
BspACI CCGC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 691
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 2 cut(s) 12, 372
BspT107I GGYRCC 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 4 cut(s) 4, 364, 464, 738
BssNI GRCGYC 1 cut(s) 227
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 539
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 62, 670, 711
Bst6I CTCTTC 3 cut(s) 60, 65, 579
BstACI GRCGYC 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 617
BstENI CCTNNNNNAGG 1 cut(s) 538
BstKTI GATC 4 cut(s) 7, 367, 467, 741
BstMAI GTCTC 3 cut(s) 176, 358, 456
BstMBI GATC 4 cut(s) 4, 364, 464, 738
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstSFI CTRYAG 1 cut(s) 460
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 2 cut(s) 646, 649
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 4, 738
BstYI RGATCY 2 cut(s) 4, 738
BtsIMutI CAGTG 2 cut(s) 67, 744
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 223
CseI GACGC 2 cut(s) 216, 380
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 53, 130, 427
CviJI RGCY 5 cut(s) 236, 274, 291, 567, 616
CviKI_1 RGCY 5 cut(s) 236, 274, 291, 567, 616
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 617
DpnI GATC 4 cut(s) 6, 366, 466, 740
DpnII GATC 4 cut(s) 4, 364, 464, 738
DrdI GACNNNNNNGTC 1 cut(s) 758
DseDI GACNNNNNNGTC 1 cut(s) 758
Eam1104I CTCTTC 3 cut(s) 60, 65, 579
EarI CTCTTC 3 cut(s) 60, 65, 579
Eco130I CCWWGG 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 539
EcoT22I ATGCAT 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 3 cut(s) 56, 133, 430
FaiI YATR 5 cut(s) 54, 131, 143, 428, 479
FatI CATG 3 cut(s) 52, 129, 426
Fnu4HI GCNGC 2 cut(s) 660, 663
Fsp4HI GCNGC 2 cut(s) 660, 663
FspBI CTAG 1 cut(s) 162
GluI GCNGC 2 cut(s) 660, 663
HapII CCGG 1 cut(s) 224
HgaI GACGC 2 cut(s) 216, 380
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 3 cut(s) 56, 133, 430
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 289
HinfI GANTC 4 cut(s) 440, 575, 630, 727
HpaII CCGG 1 cut(s) 224
HphI GGTGA 3 cut(s) 144, 459, 523
Hpy166II GTNNAC 2 cut(s) 391, 556
Hpy188I TCNGA 3 cut(s) 106, 583, 635
Hpy188III TCNNGA 8 cut(s) 8, 44, 179, 368, 397, 524, 610, 736
Hpy8I GTNNAC 2 cut(s) 391, 556
Hpy99I CGWCG 2 cut(s) 198, 232
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 3 cut(s) 62, 670, 711
HpyCH4V TGCA 2 cut(s) 462, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 617
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 3 cut(s) 56, 133, 430
Kzo9I GATC 4 cut(s) 4, 364, 464, 738
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 2 cut(s) 646, 649
LweI GCATC 2 cut(s) 25, 490
MaeI CTAG 1 cut(s) 162
MalI GATC 4 cut(s) 6, 366, 466, 740
MboI GATC 4 cut(s) 4, 364, 464, 738
MboII GAAGA 9 cut(s) 68, 77, 82, 90, 93, 332, 341, 344, 596
MflI RGATCY 2 cut(s) 4, 738
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 9 cut(s) 109, 244, 267, 402, 416, 514, 639, 767, 772
MlyI GAGTC 2 cut(s) 434, 584
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 5 cut(s) 66, 294, 313, 544, 693
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 2 cut(s) 137, 731
MseI TTAA 3 cut(s) 243, 771, 775
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 4 cut(s) 4, 364, 464, 738
NlaIII CATG 3 cut(s) 56, 133, 430
NlaIV GGNNCC 1 cut(s) 691
NsiI ATGCAT 1 cut(s) 483
PacI TTAATTAA 1 cut(s) 775
PaqCI CACCTGC 1 cut(s) 467
PdmI GAANNNNTTC 2 cut(s) 137, 731
PfeI GAWTC 2 cut(s) 630, 727
PkrI GCNGC 2 cut(s) 661, 664
PleI GAGTC 2 cut(s) 434, 583
PpsI GAGTC 2 cut(s) 434, 583
PshBI ATTAAT 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 691
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 4, 738
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SaqAI TTAA 3 cut(s) 243, 771, 775
SatI GCNGC 2 cut(s) 660, 663
Sau3AI GATC 4 cut(s) 4, 364, 464, 738
SchI GAGTC 2 cut(s) 434, 584
SduI GDGCHC 1 cut(s) 694
SfaNI GCATC 2 cut(s) 25, 490
SfcI CTRYAG 1 cut(s) 460
SgrAI CRCCGGYG 1 cut(s) 223
Sse9I AATT 9 cut(s) 109, 244, 267, 402, 416, 514, 639, 767, 772
SsiI CCGC 1 cut(s) 203
SspI AATATT 1 cut(s) 435
SspMI CTAG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 3 cut(s) 62, 670, 711
TaqI TCGA 1 cut(s) 83
TasI AATT 9 cut(s) 109, 244, 267, 402, 416, 514, 639, 767, 772
TfiI GAWTC 2 cut(s) 630, 727
Tru1I TTAA 3 cut(s) 243, 771, 775
Tru9I TTAA 3 cut(s) 243, 771, 775
TscAI CASTG 2 cut(s) 67, 751
TseI GCWGC 2 cut(s) 659, 662
TspDTI ATGAA 5 cut(s) 69, 130, 146, 429, 443
TspRI CASTG 2 cut(s) 67, 751
VspI ATTAAT 1 cut(s) 243
XagI CCTNNNNNAGG 1 cut(s) 538
XapI RAATTY 3 cut(s) 402, 416, 767
XmnI GAANNNNTTC 2 cut(s) 137, 731
XspI CTAG 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.