MD14G1003600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009971
Physical Location & Seq
Forward (+)
425777 .. 427646
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1003600.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGATCTTGATGAGTGGGAATACCTCCCTGACGATGCGTTTCTTGATTTCCATGAAGAGAGTGCGGACAAGAGGATTTTCTCAAGCAAGCGATACAACTCTTCTGATAATTCAAAAAGTGTTTTCAACATGAACTACTTCATAATCCCATCACCCAGTTCTAGGTCAAAAGTCATTCACGAGACACCACCACCACAACCGCCAACACCACCGCCATCGGCGTCGCCAGCTGGGATTGATTCAAGGCTTGTTCCTGTTCCAATTCAACTGGAACTACCTCCAAGCTTTTGCAAGGCCTCATCACTCCCACAAGAAGAACAAGAAGAAAAACAAGTAATCAAAGTCTCCCAAGTGGATCAAGACACACAACTGTCCCAAGTTTACTTCAAGAAAACTTGGGAAAATGAATTTGCCGACATGAAAATATTGGACTCCCCAAAGTCTCCCACCTGCAAATCACCCAAGTTTATGCATCAACAAACTGATAAGTTTCAGTTTGATGAAAATTTAGGTCAAGAAATCACCTCTCCAAGGACGAAAGTTGTTGACCAAAACAGCTTGTTGGAGTCATCAGAAGATACCAAAGAGATCAATAACAGCAACAACAACAACAACAGCAACAACATCTGGAAGCTGAGTTTTACAGGAATCGGTGCGATTTGCTCTTTTGGTTTTGCTGCTGCTACCATTTGTATTATCTTTTTCGGTGCCCATCAGAGGAACAAACAGTATCAGCACAACCAGAATCGCTTCCAGATCTATACTGATGACAACAAGAGGTTTAATCAGGCTATGGAACGTCCAACCAAATTGAATGAAGCATTTTCAGCAGCTAGAGGAGTACCCCTCCCTAGAGCTCATGTCACATATGGTGGTTACTATAATGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.8

Weight (kDa)

5.59

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 119 - 297 1.9e-56 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 707
AciI CCGC 3 cut(s) 65, 200, 212
AclWI GGATC 2 cut(s) 12, 363
AcsI RAATTY 2 cut(s) 407, 505
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 1 cut(s) 843
AfiI CCNNNNNNNGG 3 cut(s) 162, 531, 717
AgsI TTSAA 6 cut(s) 114, 127, 243, 266, 388, 814
AluBI AGCT 6 cut(s) 230, 285, 558, 634, 833, 857
AluI AGCT 6 cut(s) 230, 285, 558, 634, 833, 857
Alw21I GWGCWC 1 cut(s) 859
Alw26I GTCTC 3 cut(s) 176, 349, 447
AlwI GGATC 2 cut(s) 12, 363
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 677, 680, 830
ApoI RAATTY 2 cut(s) 407, 505
Asp700I GAANNNNTTC 2 cut(s) 137, 749
AsuHPI GGTGA 3 cut(s) 144, 450, 514
BaeGI GKGCMC 1 cut(s) 712
BanI GGYRCC 1 cut(s) 707
BanII GRGCYC 1 cut(s) 859
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 3 cut(s) 664, 667, 842
BccI CCATC 3 cut(s) 157, 223, 720
BcoDI GTCTC 3 cut(s) 176, 349, 447
BfaI CTAG 3 cut(s) 162, 834, 852
BfuAI ACCTGC 1 cut(s) 458
BglII AGATCT 1 cut(s) 756
BisI GCNGC 3 cut(s) 678, 681, 831
BlsI GCNGC 3 cut(s) 679, 682, 832
BmiI GGNNCC 1 cut(s) 709
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 25, 481
BmuI ACTGGG 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 831, 863
BpuEI CTTGAG 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 530
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 531, 717
Bse1I ACTGG 2 cut(s) 156, 273
BseDI CCNNGG 1 cut(s) 530
BseLI CCNNNNNNNGG 3 cut(s) 162, 531, 717
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 2 cut(s) 156, 273
BseRI GAGGAG 1 cut(s) 852
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 3 cut(s) 664, 667, 842
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 1 cut(s) 296
BshNI GGYRCC 1 cut(s) 707
BsiHKAI GWGCWC 1 cut(s) 859
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 3 cut(s) 162, 531, 717
BsmAI GTCTC 3 cut(s) 176, 349, 447
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 712, 859
Bsp143I GATC 4 cut(s) 4, 355, 588, 756
BspACI CCGC 3 cut(s) 65, 200, 212
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 709
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 2 cut(s) 12, 363
BspT107I GGYRCC 1 cut(s) 707
BsrI ACTGG 2 cut(s) 156, 273
BssECI CCNNGG 1 cut(s) 530
BssMI GATC 4 cut(s) 4, 355, 588, 756
BssNI GRCGYC 1 cut(s) 221
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 530
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 372, 729
Bst6I CTCTTC 2 cut(s) 51, 106
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 89, 228
BstDEI CTNAG 1 cut(s) 635
BstENI CCTNNNNNAGG 1 cut(s) 529
BstKTI GATC 4 cut(s) 7, 358, 591, 759
BstMAI GTCTC 3 cut(s) 176, 349, 447
BstMBI GATC 4 cut(s) 4, 355, 588, 756
BstMWI GCNNNNNNNGC 2 cut(s) 227, 827
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 3 cut(s) 664, 667, 842
BstX2I RGATCY 2 cut(s) 4, 756
BstYI RGATCY 2 cut(s) 4, 756
BsuRI GGCC 1 cut(s) 296
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 2 cut(s) 89, 228
CseI GACGC 1 cut(s) 210
Csp6I GTAC 1 cut(s) 842
CviAII CATG 4 cut(s) 53, 130, 418, 860
CviJI RGCY 9 cut(s) 230, 247, 285, 296, 558, 634, 791, 833, 857
CviKI_1 RGCY 9 cut(s) 230, 247, 285, 296, 558, 634, 791, 833, 857
CviQI GTAC 1 cut(s) 842
DdeI CTNAG 1 cut(s) 635
DpnI GATC 4 cut(s) 6, 357, 590, 758
DpnII GATC 4 cut(s) 4, 355, 588, 756
Eam1104I CTCTTC 2 cut(s) 51, 106
EarI CTCTTC 2 cut(s) 51, 106
Ecl136II GAGCTC 1 cut(s) 857
Eco130I CCWWGG 1 cut(s) 530
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 859
Eco53kI GAGCTC 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 857
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 530
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 530
FaeI CATG 4 cut(s) 56, 133, 421, 863
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 52, 129, 417, 859
FauNDI CATATG 1 cut(s) 868
Fnu4HI GCNGC 3 cut(s) 678, 681, 831
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 3 cut(s) 678, 681, 831
FspBI CTAG 3 cut(s) 162, 834, 852
GluI GCNGC 3 cut(s) 678, 681, 831
GsaI CCCAGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 296
HgaI GACGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 4 cut(s) 56, 133, 421, 863
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 1 cut(s) 283
HinfI GANTC 5 cut(s) 239, 431, 566, 648, 745
HphI GGTGA 3 cut(s) 144, 450, 514
Hpy166II GTNNAC 2 cut(s) 382, 547
Hpy188I TCNGA 3 cut(s) 106, 574, 717
Hpy188III TCNNGA 8 cut(s) 8, 44, 179, 359, 388, 515, 628, 754
Hpy8I GTNNAC 2 cut(s) 382, 547
Hpy99I CGWCG 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 372, 729
HpyCH4IV ACGT 1 cut(s) 799
HpyCH4V TGCA 3 cut(s) 291, 453, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 827
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 1 cut(s) 799
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 4 cut(s) 56, 133, 421, 863
Kzo9I GATC 4 cut(s) 4, 355, 588, 756
Lsp1109I GCAGC 3 cut(s) 664, 667, 842
LweI GCATC 2 cut(s) 25, 481
MaeI CTAG 3 cut(s) 162, 834, 852
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 2 cut(s) 862, 875
MalI GATC 4 cut(s) 6, 357, 590, 758
MboI GATC 4 cut(s) 4, 355, 588, 756
MboII GAAGA 5 cut(s) 68, 93, 326, 335, 587
MflI RGATCY 2 cut(s) 4, 756
MhlI GDGCHC 2 cut(s) 712, 859
MluCI AATT 5 cut(s) 109, 261, 407, 505, 809
MlyI GAGTC 2 cut(s) 425, 575
MmeI TCCRAC 2 cut(s) 543, 827
MnlI CCTC 9 cut(s) 35, 66, 288, 307, 535, 711, 771, 830, 857
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 2 cut(s) 137, 749
MseI TTAA 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 227, 827
NdeI CATATG 1 cut(s) 868
NdeII GATC 4 cut(s) 4, 355, 588, 756
NlaIII CATG 4 cut(s) 56, 133, 421, 863
NlaIV GGNNCC 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 862
NsiI ATGCAT 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 458
PceI AGGCCT 1 cut(s) 296
PdmI GAANNNNTTC 2 cut(s) 137, 749
PfeI GAWTC 3 cut(s) 239, 648, 745
PkrI GCNGC 3 cut(s) 679, 682, 832
PleI GAGTC 2 cut(s) 425, 574
PpsI GAGTC 2 cut(s) 425, 574
Psp124BI GAGCTC 1 cut(s) 859
PspFI CCCAGC 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 709
PsuI RGATCY 2 cut(s) 4, 756
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 843
RsaNI GTAC 1 cut(s) 842
SacI GAGCTC 1 cut(s) 859
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 3 cut(s) 678, 681, 831
Sau3AI GATC 4 cut(s) 4, 355, 588, 756
SchI GAGTC 2 cut(s) 425, 575
SduI GDGCHC 2 cut(s) 712, 859
SfaNI GCATC 2 cut(s) 25, 481
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 5 cut(s) 109, 261, 407, 505, 809
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 3 cut(s) 65, 200, 212
SspI AATATT 1 cut(s) 426
SspMI CTAG 3 cut(s) 162, 834, 852
SstI GAGCTC 1 cut(s) 859
StuI AGGCCT 1 cut(s) 296
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 2 cut(s) 372, 729
TaiI ACGT 1 cut(s) 802
TasI AATT 5 cut(s) 109, 261, 407, 505, 809
TfiI GAWTC 3 cut(s) 239, 648, 745
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 3 cut(s) 677, 680, 830
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 7 cut(s) 69, 130, 146, 420, 434, 516, 831
XagI CCTNNNNNAGG 1 cut(s) 529
XapI RAATTY 2 cut(s) 407, 505
XmnI GAANNNNTTC 2 cut(s) 137, 749
XspI CTAG 3 cut(s) 162, 834, 852
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.