pycom14g00160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009971
Physical Location & Seq
Forward (+)
87150 .. 88480
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00160.1

Sequence Viewer

Length: 882 bp
ATGGATCTTGATGAGTGGGAATACCTCCCTGACGATGCGTTTCTTGATTTCCATGAAGAGAGTGCGGACAAGAGGATTTTCTCAAGCAAGCGATACTCCTCTTCTGATAATTCAAAAAGTGTATTCAACATGAACTACTTCATAATCCCATCACCCAGTTCTAGGTCAAAAGTCATTCACGAGACACCACCACCACAACCGCCAACACCACCGCCATCGGCGTCGCCAGCTGGGATTGATTCAAGGCTTGTTCCGGTTCCAATTCAACTGGAACTGCCTCCAAGCTTTGGCAAGGCCTCATCACTCCCACAAGAAGAAGAAAAACAAGTCATCAAAGTCTCCCAAGTGGATCAAGACACACAACTGTCCCAAGTTTACTTCAAGAAAACTTGGGAAAATGAATTTGCCGACATGAAAATATTGGACTCCCCAAAGTCTCCCACCTGCAGATCACCCAAGTTTATGCATCAACAAACTGATAAGCTTCAGTTTGATGAAAATTTAGGTCAAGAAATCACCTCTCCAAGGACGAAAGTTGTTGACCAAAACAGCTTGTTGGAGTCATCAGAAGATACCAAAGAGATCAACAACAGCAACAACAACAACAACAACATCTGGAAGCTGAGTTTTACAGGAATCGGAGCGATTTGCTCTTTTGGTTTTGCTGCTGCTACCATTTGTATTATCTTTTTCGGTGCCCATCAGAGGAACAAACAGTATCAGCACAACCAGAATCGCTTCCAGATCTATACTGATGACAACAAGAGGATTAAGCAGGCTGTTGAACATGCAACCAAATTGAATGAAGCATTTTCAGCAGCTAGAGGAGTACCCCTCCCTAGAGCTCATGTCACATATGGTGGTTACTATAATGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

33.17

Weight (kDa)

5.84

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 117 - 293 1.2e-59 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 695
AccB7I CCANNNNNTGG 1 cut(s) 287
AciI CCGC 3 cut(s) 65, 200, 212
AclWI GGATC 2 cut(s) 12, 357
AcsI RAATTY 2 cut(s) 401, 499
AcuI CTGAAG 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 1 cut(s) 831
AfiI CCNNNNNNNGG 4 cut(s) 162, 287, 525, 705
AgsI TTSAA 7 cut(s) 114, 127, 243, 266, 382, 785, 802
AluBI AGCT 7 cut(s) 230, 285, 484, 552, 622, 821, 845
AluI AGCT 7 cut(s) 230, 285, 484, 552, 622, 821, 845
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 3 cut(s) 176, 343, 441
AlwI GGATC 2 cut(s) 12, 357
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 665, 668, 818
ApoI RAATTY 2 cut(s) 401, 499
Asp700I GAANNNNTTC 2 cut(s) 137, 737
AsuHPI GGTGA 3 cut(s) 144, 444, 508
BaeGI GKGCMC 1 cut(s) 700
BanI GGYRCC 1 cut(s) 695
BanII GRGCYC 1 cut(s) 847
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 3 cut(s) 652, 655, 830
BccI CCATC 3 cut(s) 157, 223, 708
BcoDI GTCTC 3 cut(s) 176, 343, 441
BfaI CTAG 3 cut(s) 162, 822, 840
BfmI CTRYAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 452
BglII AGATCT 1 cut(s) 744
BisI GCNGC 3 cut(s) 666, 669, 819
BlsI GCNGC 3 cut(s) 667, 670, 820
BmiI GGNNCC 2 cut(s) 258, 697
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 25, 475
BmuI ACTGGG 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 819, 851
BpuEI CTTGAG 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 524
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 287, 525, 705
Bse1I ACTGG 2 cut(s) 156, 273
BseDI CCNNGG 1 cut(s) 524
BseLI CCNNNNNNNGG 4 cut(s) 162, 287, 525, 705
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 2 cut(s) 156, 273
BseRI GAGGAG 2 cut(s) 88, 840
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 3 cut(s) 652, 655, 830
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 1 cut(s) 296
BshNI GGYRCC 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 847
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 4 cut(s) 162, 287, 525, 705
BsmAI GTCTC 3 cut(s) 176, 343, 441
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 700, 847
Bsp143I GATC 5 cut(s) 4, 349, 449, 582, 744
BspACI CCGC 3 cut(s) 65, 200, 212
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 258, 697
BspMAI CTGCAG 1 cut(s) 449
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 2 cut(s) 12, 357
BspT107I GGYRCC 1 cut(s) 695
BsrI ACTGG 2 cut(s) 156, 273
BssECI CCNNGG 1 cut(s) 524
BssMI GATC 5 cut(s) 4, 349, 449, 582, 744
BssNI GRCGYC 1 cut(s) 221
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 366, 717
Bst6I CTCTTC 2 cut(s) 51, 106
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 89, 228, 777
BstDEI CTNAG 1 cut(s) 623
BstENI CCTNNNNNAGG 1 cut(s) 523
BstKTI GATC 5 cut(s) 7, 352, 452, 585, 747
BstMAI GTCTC 3 cut(s) 176, 343, 441
BstMBI GATC 5 cut(s) 4, 349, 449, 582, 744
BstMWI GCNNNNNNNGC 2 cut(s) 227, 815
BstNSI RCATGY 1 cut(s) 791
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 700
BstV1I GCAGC 3 cut(s) 652, 655, 830
BstX2I RGATCY 2 cut(s) 4, 744
BstYI RGATCY 2 cut(s) 4, 744
BsuRI GGCC 1 cut(s) 296
BveI ACCTGC 1 cut(s) 452
Cac8I GCNNGC 3 cut(s) 89, 228, 777
CseI GACGC 1 cut(s) 210
Csp6I GTAC 1 cut(s) 830
CviAII CATG 5 cut(s) 53, 130, 412, 788, 848
CviQI GTAC 1 cut(s) 830
DdeI CTNAG 1 cut(s) 623
DpnI GATC 5 cut(s) 6, 351, 451, 584, 746
DpnII GATC 5 cut(s) 4, 349, 449, 582, 744
Eam1104I CTCTTC 2 cut(s) 51, 106
EarI CTCTTC 2 cut(s) 51, 106
Ecl136II GAGCTC 1 cut(s) 845
Eco130I CCWWGG 1 cut(s) 524
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 847
Eco53kI GAGCTC 1 cut(s) 845
Eco57I CTGAAG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 845
EcoNI CCTNNNNNAGG 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 524
EcoT22I ATGCAT 1 cut(s) 468
EcoT38I GRGCYC 1 cut(s) 847
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 5 cut(s) 56, 133, 415, 791, 851
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 52, 129, 411, 787, 847
FauNDI CATATG 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 666, 669, 819
FriOI GRGCYC 1 cut(s) 847
Fsp4HI GCNGC 3 cut(s) 666, 669, 819
FspBI CTAG 3 cut(s) 162, 822, 840
GluI GCNGC 3 cut(s) 666, 669, 819
GsaI CCCAGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 296
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 5 cut(s) 56, 133, 415, 791, 851
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 2 cut(s) 283, 482
HinfI GANTC 5 cut(s) 239, 425, 560, 636, 733
HpaII CCGG 1 cut(s) 254
HphI GGTGA 3 cut(s) 144, 444, 508
Hpy166II GTNNAC 2 cut(s) 376, 541
Hpy188I TCNGA 4 cut(s) 106, 568, 641, 705
Hpy188III TCNNGA 8 cut(s) 8, 44, 179, 353, 382, 509, 616, 742
Hpy8I GTNNAC 2 cut(s) 376, 541
Hpy99I CGWCG 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 366, 717
HpyCH4V TGCA 3 cut(s) 447, 466, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 815
HpyF3I CTNAG 1 cut(s) 623
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 5 cut(s) 56, 133, 415, 791, 851
Kzo9I GATC 5 cut(s) 4, 349, 449, 582, 744
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 3 cut(s) 652, 655, 830
LweI GCATC 2 cut(s) 25, 475
MaeI CTAG 3 cut(s) 162, 822, 840
MaeIII GTNAC 2 cut(s) 850, 863
MalI GATC 5 cut(s) 6, 351, 451, 584, 746
MboI GATC 5 cut(s) 4, 349, 449, 582, 744
MboII GAAGA 5 cut(s) 68, 93, 326, 329, 581
MflI RGATCY 2 cut(s) 4, 744
MhlI GDGCHC 2 cut(s) 700, 847
MluCI AATT 5 cut(s) 109, 261, 401, 499, 797
MlyI GAGTC 2 cut(s) 419, 569
MmeI TCCRAC 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 468
MroXI GAANNNNTTC 2 cut(s) 137, 737
MseI TTAA 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 227, 815
NdeI CATATG 1 cut(s) 856
NdeII GATC 5 cut(s) 4, 349, 449, 582, 744
NlaIII CATG 5 cut(s) 56, 133, 415, 791, 851
NlaIV GGNNCC 2 cut(s) 258, 697
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 791
PaqCI CACCTGC 1 cut(s) 452
PceI AGGCCT 1 cut(s) 296
PdmI GAANNNNTTC 2 cut(s) 137, 737
PfeI GAWTC 3 cut(s) 239, 636, 733
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 3 cut(s) 667, 670, 820
PleI GAGTC 2 cut(s) 419, 568
PpsI GAGTC 2 cut(s) 419, 568
Psp124BI GAGCTC 1 cut(s) 847
PspFI CCCAGC 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 258, 697
PstI CTGCAG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 4, 744
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 831
RsaNI GTAC 1 cut(s) 830
SacI GAGCTC 1 cut(s) 847
SaqAI TTAA 1 cut(s) 771
SatI GCNGC 3 cut(s) 666, 669, 819
Sau3AI GATC 5 cut(s) 4, 349, 449, 582, 744
SchI GAGTC 2 cut(s) 419, 569
SduI GDGCHC 2 cut(s) 700, 847
SfaNI GCATC 2 cut(s) 25, 475
SfcI CTRYAG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 5 cut(s) 109, 261, 401, 499, 797
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 3 cut(s) 65, 200, 212
SspI AATATT 1 cut(s) 420
SspMI CTAG 3 cut(s) 162, 822, 840
SstI GAGCTC 1 cut(s) 847
StuI AGGCCT 1 cut(s) 296
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 2 cut(s) 366, 717
TasI AATT 5 cut(s) 109, 261, 401, 499, 797
TfiI GAWTC 3 cut(s) 239, 636, 733
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 3 cut(s) 665, 668, 818
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 7 cut(s) 69, 130, 146, 414, 428, 510, 819
Van91I CCANNNNNTGG 1 cut(s) 287
XagI CCTNNNNNAGG 1 cut(s) 523
XapI RAATTY 2 cut(s) 401, 499
XceI RCATGY 1 cut(s) 791
XmnI GAANNNNTTC 2 cut(s) 137, 737
XspI CTAG 3 cut(s) 162, 822, 840
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.