MD12G1005100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009971
Physical Location & Seq
Forward (+)
641272 .. 643202
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1005100.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGATCTTGATGAGTGGGAGTACCTACCAGATGATGCGTTTCTTGATTTCCATGAAGACAGTGAAGAGAAGAGGATTTTCTCGAGCAAACGCTATTCTTCTTCTGATAATTCAAAAAGTGTTTTCAACATGAACTACTTCATAATCCCATCACCCAGTTCTAGGTCAAAAGTCATTCACGAGACACCACCACGACGACTACCGCCAACAACACCGCCACCGGTGTCGCCAGCTTGGATTAATTCAAGTCTTGTTCCCGTTCCAATTCAGCTGGAACTACCTCCAAGCCTTGACAAGGCCCCATCACTCTCACAAGAAGAACAAGAAGAAGAAAAACAAGTCATCAAAGTCTCCCAAGTGGATCAAGACGCACAACTCTCCCAAGTTTACTTCAAGAAAACTTGGGAAAATGAATTTGCCGACATGAAAATATTGGACTCCCCAAAGTCTCCCACCTGCAGATCACCTATGCATCAACAAACTGATAAGTTTCAGTTTGATGACAATTTAGGTCAAGAAATCACCTCTCTAAGGAAGGAAGTTGTTGACAAAAACAGCTTGTTGGAGTCATCAGAAGAGATAAATAGCAACAACAACAACAACAACATCTGGAAGCTGAGTTTTACAGGAATCGGAGCAATTTGCTCTTTTGGTTTTGCTGCTGCTACCGTTTGTATTATCTTTTTCGGTGCCCATCAGAGGAACAAACAGTATCAGCACAACCAGAATCGCTTCCAGATCTACACTGACGACAACAAGAGGATTAAGCATGCTGTTCAAGATGCAACCAAATTGAATGAAGCATTTTCAGCAGTTAGAGTATCCCTCCCTAGAGCTCATGTCACATATGGTGGTTACTATAATGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

33.17

Weight (kDa)

5.44

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 122 - 290 1.9e-53 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 689
AciI CCGC 2 cut(s) 203, 215
AclWI GGATC 2 cut(s) 12, 369
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 4 cut(s) 162, 295, 531, 699
AgeI ACCGGT 1 cut(s) 220
AgsI TTSAA 6 cut(s) 114, 127, 246, 394, 779, 796
AluBI AGCT 5 cut(s) 233, 271, 558, 616, 836
AluI AGCT 5 cut(s) 233, 271, 558, 616, 836
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 3 cut(s) 176, 355, 453
AlwI GGATC 2 cut(s) 12, 369
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 659, 662
ApoI RAATTY 1 cut(s) 413
AseI ATTAAT 1 cut(s) 240
AsiGI ACCGGT 1 cut(s) 220
Asp700I GAANNNNTTC 2 cut(s) 137, 731
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 144, 456, 514
AvaI CYCGRG 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 689
BanII GRGCYC 1 cut(s) 838
BauI CACGAG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 2 cut(s) 646, 649
BccI CCATC 3 cut(s) 157, 310, 702
BciVI GTATCC 1 cut(s) 832
BcoDI GTCTC 3 cut(s) 176, 355, 453
BfaI CTAG 2 cut(s) 162, 831
BfmI CTRYAG 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 464
BfuI GTATCC 1 cut(s) 832
BglII AGATCT 1 cut(s) 738
BisI GCNGC 2 cut(s) 660, 663
BlsI GCNGC 2 cut(s) 661, 664
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 300, 691
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 3 cut(s) 25, 481, 772
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 810, 842
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 295, 531, 699
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 156
BseLI CCNNNNNNNGG 4 cut(s) 162, 295, 531, 699
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 2 cut(s) 646, 649
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 689
BshTI ACCGGT 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 4 cut(s) 162, 295, 531, 699
BsmAI GTCTC 3 cut(s) 176, 355, 453
BsnI GGCC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 694, 838
Bsp143I GATC 4 cut(s) 4, 361, 461, 738
BspACI CCGC 2 cut(s) 203, 215
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 1 cut(s) 609
BspLI GGNNCC 2 cut(s) 300, 691
BspMAI CTGCAG 1 cut(s) 461
BspMI ACCTGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 12, 369
BspT107I GGYRCC 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 220
BssMI GATC 4 cut(s) 4, 361, 461, 738
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 62, 670, 711
Bst6I CTCTTC 3 cut(s) 60, 65, 570
BstC8I GCNNGC 2 cut(s) 231, 771
BstDEI CTNAG 2 cut(s) 530, 617
BstENI CCTNNNNNAGG 2 cut(s) 293, 529
BstKTI GATC 4 cut(s) 7, 364, 464, 741
BstMAI GTCTC 3 cut(s) 176, 355, 453
BstMBI GATC 4 cut(s) 4, 361, 461, 738
BstMWI GCNNNNNNNGC 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 773
BstSFI CTRYAG 1 cut(s) 457
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 2 cut(s) 646, 649
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 4, 738
BstYI RGATCY 2 cut(s) 4, 738
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 1 cut(s) 299
BtsIMutI CAGTG 2 cut(s) 67, 744
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 2 cut(s) 231, 771
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 22
CspAI ACCGGT 1 cut(s) 220
CviAII CATG 5 cut(s) 53, 130, 424, 770, 839
CviJI RGCY 7 cut(s) 233, 271, 288, 299, 558, 616, 836
CviKI_1 RGCY 7 cut(s) 233, 271, 288, 299, 558, 616, 836
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 2 cut(s) 530, 617
DpnI GATC 4 cut(s) 6, 363, 463, 740
DpnII GATC 4 cut(s) 4, 361, 461, 738
Eam1104I CTCTTC 3 cut(s) 60, 65, 570
EarI CTCTTC 3 cut(s) 60, 65, 570
Ecl136II GAGCTC 1 cut(s) 836
Eco24I GRGCYC 1 cut(s) 838
Eco53kI GAGCTC 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 836
EcoNI CCTNNNNNAGG 2 cut(s) 293, 529
EcoO109I RGGNCCY 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 838
FaeI CATG 5 cut(s) 56, 133, 427, 773, 842
FatI CATG 5 cut(s) 52, 129, 423, 769, 838
FauNDI CATATG 1 cut(s) 847
Fnu4HI GCNGC 2 cut(s) 660, 663
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 2 cut(s) 660, 663
FspBI CTAG 2 cut(s) 162, 831
GluI GCNGC 2 cut(s) 660, 663
HaeIII GGCC 1 cut(s) 299
HapII CCGG 1 cut(s) 221
HgaI GACGC 1 cut(s) 377
Hin1II CATG 5 cut(s) 56, 133, 427, 773, 842
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 4 cut(s) 437, 566, 630, 727
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 144, 456, 514
Hpy166II GTNNAC 2 cut(s) 388, 547
Hpy188I TCNGA 4 cut(s) 106, 574, 635, 699
Hpy8I GTNNAC 2 cut(s) 388, 547
Hpy99I CGWCG 1 cut(s) 198
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 3 cut(s) 62, 670, 711
HpyCH4V TGCA 3 cut(s) 459, 472, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 809
HpyF3I CTNAG 2 cut(s) 530, 617
Hsp92II CATG 5 cut(s) 56, 133, 427, 773, 842
Kzo9I GATC 4 cut(s) 4, 361, 461, 738
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 2 cut(s) 646, 649
LweI GCATC 3 cut(s) 25, 481, 772
MaeI CTAG 2 cut(s) 162, 831
MaeIII GTNAC 2 cut(s) 841, 854
MalI GATC 4 cut(s) 6, 363, 463, 740
MboI GATC 4 cut(s) 4, 361, 461, 738
MboII GAAGA 9 cut(s) 68, 77, 82, 90, 93, 329, 338, 341, 587
MflI RGATCY 2 cut(s) 4, 738
MhlI GDGCHC 2 cut(s) 694, 838
MluCI AATT 7 cut(s) 109, 241, 264, 413, 505, 639, 791
MlyI GAGTC 2 cut(s) 431, 575
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 6 cut(s) 66, 291, 535, 693, 753, 836
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 2 cut(s) 137, 731
MseI TTAA 2 cut(s) 240, 765
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 809
NdeI CATATG 1 cut(s) 847
NdeII GATC 4 cut(s) 4, 361, 461, 738
NlaIII CATG 5 cut(s) 56, 133, 427, 773, 842
NlaIV GGNNCC 2 cut(s) 300, 691
NmuCI GTSAC 1 cut(s) 841
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 1 cut(s) 773
PaeI GCATGC 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 464
PdmI GAANNNNTTC 2 cut(s) 137, 731
PfeI GAWTC 2 cut(s) 630, 727
PinAI ACCGGT 1 cut(s) 220
PkrI GCNGC 2 cut(s) 661, 664
PleI GAGTC 2 cut(s) 431, 574
PpsI GAGTC 2 cut(s) 431, 574
PshBI ATTAAT 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 838
PspN4I GGNNCC 2 cut(s) 300, 691
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 4, 738
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SacI GAGCTC 1 cut(s) 838
SaqAI TTAA 2 cut(s) 240, 765
SatI GCNGC 2 cut(s) 660, 663
Sau3AI GATC 4 cut(s) 4, 361, 461, 738
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 2 cut(s) 431, 575
SduI GDGCHC 2 cut(s) 694, 838
SfaNI GCATC 3 cut(s) 25, 481, 772
SfcI CTRYAG 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SphI GCATGC 1 cut(s) 773
Sse9I AATT 7 cut(s) 109, 241, 264, 413, 505, 639, 791
SsiI CCGC 2 cut(s) 203, 215
SspI AATATT 1 cut(s) 432
SspMI CTAG 2 cut(s) 162, 831
SstI GAGCTC 1 cut(s) 838
TaaI ACNGT 3 cut(s) 62, 670, 711
TaqI TCGA 1 cut(s) 83
TasI AATT 7 cut(s) 109, 241, 264, 413, 505, 639, 791
TfiI GAWTC 2 cut(s) 630, 727
Tru1I TTAA 2 cut(s) 240, 765
Tru9I TTAA 2 cut(s) 240, 765
TscAI CASTG 2 cut(s) 67, 751
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 2 cut(s) 659, 662
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 6 cut(s) 69, 130, 146, 426, 440, 813
TspRI CASTG 2 cut(s) 67, 751
VspI ATTAAT 1 cut(s) 240
XagI CCTNNNNNAGG 2 cut(s) 293, 529
XapI RAATTY 1 cut(s) 413
XceI RCATGY 1 cut(s) 773
XhoI CTCGAG 1 cut(s) 82
XmnI GAANNNNTTC 2 cut(s) 137, 731
XspI CTAG 2 cut(s) 162, 831
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.