Rroxscaffold_6G00388520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009971
Physical Location & Seq
Reverse (-)
1496077 .. 1497688
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388520.1

Sequence Viewer

Length: 924 bp
ATGGATCTTGATGAGTGGGAATACCTCCCTGATGATGCTTTTCTTGATTTCCATGAAGAGAGTGAGAAGAAGGTCTTCTTTAGCAAGCGCTATTTCGATTCCAAAAGTGTTTTCAATATGTACCACGACTACTGCATAATCCAATCACCCAATTCTTCTAGTTCTAAGAAACTAGTTGCCACCGATCAAGTAGCTCCTGCTGTGCCTCCCAATCAAGTTTCTGTTCCTGTTCAAATTCAACTGCTTCCTCCACCAAGCTTTTTCAGATCCACACAATCACCAGATGAAGAAAAGCAAGAGGCTCTTGTGCCAATTTCAGCAGCAGCTGTTGTTACTGGTACTACAGGAGCAGGGGGAGGAGGAGGAGGAGGAGGTGAAGGTGAAGGTGATCAAGACCCACTACTCTCCCAAGTTTACTTCAAGAAAACCACTTGGGAACCCGAATCTGTCGACATGAAAATGGACTCGCCAAAGTCTCCTACCAGCAGAGGAGGATTAGGATCAGGATTTGTGCATCAAACTAGTAGTGATTCTACTGCTGCTGCTGGAGGAGCCAAGTTTCAGTTTGATGATTATAATGGTGAGCCCAAGGTTAGCTCTCCAAGAATGAAAGCCAACAAAGAGGAGGATGAACAAGGAGAGAGGCAGAACAATAACATCTGGAAGTTGAGCTTCACTGGAATTGGAGCTATTTGCTCTTTTGGTTTTGCTGCAGCTACCATTTGTATTCTGTTTTTGGGTAGCCACCAAAGAAACAGCCAAAACCACAAACACTTCCAGGTTTACACTGATGACAACAAGAGGATTAAGGAGGTGGTTCAGCGAGCAACCAAATTGAATGAAGCATTTTCAGCAGTAAGAGGAGGGGTACCTCTAACCAGAGCTCACATAACATCTGGAGGTTACTATACTGGTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

33.43

Weight (kDa)

5.43

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 130 - 305 5.9e-50 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03870 AT3G03870 AT5G18130 AT5G18130
fragaria_vesca FvH4_1g28230
malus_domestica MD12G1005100.v1.1 MD14G1003600.v1.1
prunus_persica Prupe.7G006100_v2.0.a1 Prupe.7G006100_v2.0.a1
pyrus_communis pycom12g00420 pycom14g00160
rosa_chinensis RchiOBHm_Chr3g0497011
rosa_laevigata RLG00000022489
rosa_multiflora Rmu_sc0000438.1_g000011
rosa_roxburghii Rroxscaffold_6G00388520
rosa_rugosa Rorug03G0288900
rosa_samantha Rh3AG333100 Rh3BG370400 Rh3CG367300 Rh3DG368600
rosa_wichuraiana Rw3G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
Acc65I GGTACC 1 cut(s) 868
AccB1I GGYRCC 1 cut(s) 868
AccI GTMKAC 1 cut(s) 450
AclWI GGATC 3 cut(s) 12, 261, 508
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 3 cut(s) 122, 340, 870
AfeI AGCGCT 1 cut(s) 89
AgsI TTSAA 5 cut(s) 115, 233, 239, 421, 838
AhlI ACTAGT 2 cut(s) 172, 521
AjnI CCWGG 1 cut(s) 777
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 8 cut(s) 194, 258, 326, 597, 672, 689, 716, 884
AluI AGCT 8 cut(s) 194, 258, 326, 597, 672, 689, 716, 884
Alw21I GWGCWC 2 cut(s) 886, 919
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 3 cut(s) 12, 261, 508
AlwNI CAGNNNCTG 1 cut(s) 326
Aor51HI AGCGCT 1 cut(s) 89
ApeKI GCWGC 6 cut(s) 320, 323, 539, 542, 710, 713
ApoI RAATTY 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 74
Asp718I GGTACC 1 cut(s) 868
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 6 cut(s) 138, 270, 386, 392, 398, 593
BanI GGYRCC 1 cut(s) 868
BanII GRGCYC 2 cut(s) 588, 886
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 2 cut(s) 886, 919
BbvI GCAGC 6 cut(s) 332, 335, 526, 529, 697, 725
BciT130I CCWGG 1 cut(s) 779
BclI TGATCA 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 480
BcuI ACTAGT 2 cut(s) 172, 521
BfaI CTAG 3 cut(s) 159, 173, 522
BfmI CTRYAG 2 cut(s) 342, 711
BfoI RGCGCY 1 cut(s) 91
BisI GCNGC 6 cut(s) 321, 324, 540, 543, 711, 714
BlsI GCNGC 6 cut(s) 322, 325, 541, 544, 712, 715
Bme1390I CCNGG 1 cut(s) 779
BmiI GGNNCC 3 cut(s) 438, 553, 870
BmrFI CCNGG 1 cut(s) 779
BmsI GCATC 2 cut(s) 25, 523
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 2 cut(s) 567, 918
BsaBI GATNNNNATC 1 cut(s) 499
BsaJI CCNNGG 1 cut(s) 588
Bse1I ACTGG 3 cut(s) 340, 682, 916
Bse8I GATNNNNATC 1 cut(s) 499
BseBI CCWGG 1 cut(s) 779
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 634
BseJI GATNNNNATC 1 cut(s) 499
BseNI ACTGG 3 cut(s) 340, 682, 916
BseRI GAGGAG 9 cut(s) 372, 375, 378, 381, 384, 504, 564, 638, 876
BseXI GCAGC 6 cut(s) 332, 335, 526, 529, 697, 725
BshNI GGYRCC 1 cut(s) 868
BsiHKAI GWGCWC 2 cut(s) 886, 919
BsmAI GTCTC 1 cut(s) 480
Bsp1286I GDGCHC 3 cut(s) 588, 886, 919
Bsp143I GATC 5 cut(s) 4, 184, 266, 388, 500
BspLI GGNNCC 3 cut(s) 438, 553, 870
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 3 cut(s) 12, 261, 508
BspT107I GGYRCC 1 cut(s) 868
BsrI ACTGG 3 cut(s) 340, 682, 916
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 5 cut(s) 4, 184, 266, 388, 500
BssT1I CCWWGG 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 779
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 86, 825
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 634
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 5 cut(s) 7, 187, 269, 391, 503
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 5 cut(s) 4, 184, 266, 388, 500
BstMWI GCNNNNNNNGC 2 cut(s) 551, 851
BstNI CCWGG 1 cut(s) 779
BstSCI CCNGG 1 cut(s) 777
BstSFI CTRYAG 2 cut(s) 342, 711
BstV1I GCAGC 6 cut(s) 332, 335, 526, 529, 697, 725
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 2 cut(s) 4, 266
BstYI RGATCY 2 cut(s) 4, 266
BtsCI GGATG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 675, 786
Cac8I GCNNGC 2 cut(s) 86, 825
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 121, 339, 869
CviAII CATG 2 cut(s) 53, 454
CviQI GTAC 3 cut(s) 121, 339, 869
DdeI CTNAG 1 cut(s) 165
DpnI GATC 5 cut(s) 6, 186, 268, 390, 502
DpnII GATC 5 cut(s) 4, 184, 266, 388, 500
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 884
Eco130I CCWWGG 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 588, 886
Eco47III AGCGCT 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 884
EcoICRI GAGCTC 1 cut(s) 884
EcoRII CCWGG 1 cut(s) 777
EcoT14I CCWWGG 1 cut(s) 588
EcoT38I GRGCYC 2 cut(s) 588, 886
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 2 cut(s) 56, 457
FaiI YATR 8 cut(s) 54, 119, 137, 455, 576, 890, 909, 922
FalI AAGNNNNNCTT 8 cut(s) 59, 91, 62, 94, 288, 320, 656, 688
FatI CATG 2 cut(s) 52, 453
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 6 cut(s) 321, 324, 540, 543, 711, 714
FokI GGATG 1 cut(s) 641
FriOI GRGCYC 2 cut(s) 588, 886
Fsp4HI GCNGC 6 cut(s) 321, 324, 540, 543, 711, 714
FspBI CTAG 3 cut(s) 159, 173, 522
GlaI GCGC 1 cut(s) 89
GluI GCNGC 6 cut(s) 321, 324, 540, 543, 711, 714
GsuI CTGGAG 2 cut(s) 567, 918
HaeII RGCGCY 1 cut(s) 91
HhaI GCGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 56, 457
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 4 cut(s) 98, 443, 464, 530
HphI GGTGA 6 cut(s) 138, 270, 386, 392, 398, 593
Hpy166II GTNNAC 3 cut(s) 415, 451, 784
Hpy188I TCNGA 1 cut(s) 266
Hpy188III TCNNGA 7 cut(s) 8, 44, 392, 421, 504, 661, 897
Hpy8I GTNNAC 3 cut(s) 415, 451, 784
HpyAV CCTTC 3 cut(s) 64, 371, 377
HpyCH4V TGCA 3 cut(s) 135, 514, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 551, 851
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 2 cut(s) 56, 457
HspAI GCGC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 872
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 5 cut(s) 4, 184, 266, 388, 500
LmnI GCTCC 4 cut(s) 199, 347, 551, 686
Lsp1109I GCAGC 6 cut(s) 332, 335, 526, 529, 697, 725
LweI GCATC 2 cut(s) 25, 523
MaeI CTAG 3 cut(s) 159, 173, 522
MaeIII GTNAC 2 cut(s) 331, 902
MalI GATC 5 cut(s) 6, 186, 268, 390, 502
MboI GATC 5 cut(s) 4, 184, 266, 388, 500
MboII GAAGA 5 cut(s) 67, 68, 79, 147, 299
MflI RGATCY 2 cut(s) 4, 266
MhlI GDGCHC 3 cut(s) 588, 886, 919
MluCI AATT 5 cut(s) 151, 234, 312, 681, 833
MlyI GAGTC 1 cut(s) 458
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 779
MvaI CCWGG 1 cut(s) 779
MwoI GCNNNNNNNGC 2 cut(s) 551, 851
NdeII GATC 5 cut(s) 4, 184, 266, 388, 500
NlaIII CATG 2 cut(s) 56, 457
NlaIV GGNNCC 3 cut(s) 438, 553, 870
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 3 cut(s) 98, 443, 530
PkrI GCNGC 6 cut(s) 322, 325, 541, 544, 712, 715
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 1 cut(s) 777
PspGI CCWGG 1 cut(s) 777
PspN4I GGNNCC 3 cut(s) 438, 553, 870
PstI CTGCAG 1 cut(s) 715
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 4, 266
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 3 cut(s) 122, 340, 870
RsaNI GTAC 3 cut(s) 121, 339, 869
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 886
SalI GTCGAC 1 cut(s) 449
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 6 cut(s) 321, 324, 540, 543, 711, 714
Sau3AI GATC 5 cut(s) 4, 184, 266, 388, 500
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 1 cut(s) 779
SduI GDGCHC 3 cut(s) 588, 886, 919
SfaNI GCATC 2 cut(s) 25, 523
SfcI CTRYAG 2 cut(s) 342, 711
SmiMI CAYNNNNRTG 1 cut(s) 458
SpeI ACTAGT 2 cut(s) 172, 521
Sse9I AATT 5 cut(s) 151, 234, 312, 681, 833
SspMI CTAG 3 cut(s) 159, 173, 522
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 1 cut(s) 777
StyI CCWWGG 1 cut(s) 588
TaqI TCGA 2 cut(s) 96, 450
TasI AATT 5 cut(s) 151, 234, 312, 681, 833
TfiI GAWTC 3 cut(s) 98, 443, 530
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 682, 793
TseI GCWGC 6 cut(s) 320, 323, 539, 542, 710, 713
TspDTI ATGAA 6 cut(s) 69, 300, 470, 623, 645, 855
TspRI CASTG 2 cut(s) 682, 793
XapI RAATTY 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 3 cut(s) 159, 173, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.