FvH4_1g28580

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009860
Physical Location & Seq
Forward (+)
20868228 .. 20888230
20003 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28580.t1

Sequence Viewer

Length: 2604 bp
ATGTTGGCAAACCCTAGCAGTGGCATACTGCGCTGCACTGCTAAACCCTTTAACTTCCACCCAAATTCAAACCCAAAACCAAAATCAAACAGGCCCAAATGTTTCGTGTGCCCACGCAGAAGCAGCAGCAGGACCAAGTATTGCAGTCTGTCCCTCCTCCTGCCCAGAAAGAATCGCAAGGCCTTCGGCATCAGAGCTTTGACGGACACTGCCGCCGTTGATGTTGTTCAGCCCTACTCCGTCAAAATCCCCGTTGGTGATAGACACATTTTGATCGAGACAGGTCATATTGGGAGACAAGCCAGTGCTTCCGTTACAGTCACTGATGGCGAAACTATTGTATACACATCTGTTTGTTTGGCTGATACTCCAAGTGAACCTTCAGACTTTTTCCCTCTTTCCGTTCATTATCAAGAGCGTTTTTCGGCAGCAGGTCGCACTAGTGGAGGCTTTTTCAAACGAGAAGGAAGGGCAAAAGATCACGAGGTTCTCATTTGCAGATTGATTGATAGGCCTTTGCGTCCAACTATGTTGAATGGCTTCTACCATGAAACTCAAGTACTTTCTTGGGTGCTGAGTTATGACGGTTTGCACTCTCCTGATTCTTTGGCAGTCACAGCAGCTGGAATAGCTGTTGCTCTTTCCGAGGTTCCAAACACAAAAACTATAGCAGGAGTTCGTATTGGTCTTGTTGGGGACAAATTTATTGTGAATCCAACAACTAAGGAGATGGAAGATTCTGAGTTGGACTTGCTGCTAGCTGGCACAGATAGTGCAATATTGATGATAGAGGGCTATTGTGATTTTCTGCCTGAGGAAAAGTTGCTGCAAGCAATAGAAGTTGGGCAGGATGCTGTACGGGCAATATGCAATCAGGTGGAGGCTTTGGTTAAGAAGTGTGGGAAGCCAAAAATGTTTGATGCGATCAAATTACCTCCCCCTGAGCTATATAATCATGTTGAGGAAATAGCCGGTGATGAGCTGGTAAGAGCTCTGCAGATTACGCACAAGACTCCTAGAAGGAAAGCCCTGTTGTCATTGGAAGGAGAAGTTTTAACATTACTTACCGAAAAGGGATATGTTTGTAAGGATACAGTTGTTGCAGGCAGTGAAACAGAACCAGATATATTTGAAGATGAAGATGAGGATGAGGAAGTGATTGTGGATGGTGAAGTAGATGAAGGTGATGTTCATATTAAGCCTACCTCGCGGAAATCCACTCCTTTGTTTTTTTCTGAGGTAGACGTGAAGCTGGTATTCAAAGAAGTAACATCCAAATATTTGCGCAGGCGCATTGTGGAGGGTGGAAGAAGAAGTGATGGGAGGACACCAGAAGAGATACGTCCAATTAACTCAAGATGTGGACTACTTCCAAGGGCACACGGAAGTGCTCTTTTTACCCGTGGCGAAACACAGTCATTGGCTGTGGTTACACTTGGTGATAAGCAGATGGCTCAGAGAATAGACAACCTCATGGGTGTAGATGAATGGAAAAGGTTCTATCTCCAGTACACATTCCCCCCTTCATGTGTTGGGGAAGTTGGACGGAATGGAGCACCTAGCAGAAGAGAAATTGGTCATGGAATGTTGGCTGAGAGATCTCTTGAACCAATATTGCCTTGTGATGATGATTTTCCTTACACTATACGTGTTGAGAGCACGATTACTGAAAGCAATGGCTCTTCAAGTATGGCATCTGTGTGTGGGGGTTGCTTAGCACTACAGGATGCTGGAGTTCCAATCAAGTGCTCTATAGTTGGAATAGCAATGGGTCTGGTTCTAGATACTGAGGAATTTGGGGGGGATGGTTCTCCTCTTATTCTCTCGGACATAACTGGATCTGAAGATGCATCAGGGGATATGGATTTCAAGGTTGCTGGAAATGAACATGGTGTAACAGCATTTCAGATGGATATAAAGGTGAGCGGGATTACATTACCAGTTATGAGAAAGGCACTCCTGCAGGCAAAAGATGGCCGAAAGCATATTATTACGGAACTGTTGAAATGCTCCCCTCCGCCTTCAGGAAGGCTTTCCAAGTATGCACCACTCATTCACGTAATGAAGGTCAAACCTGACAAAGTAAACTTAATCATTGGTTCTGGTGGGAAGAAGGTCAAAAGTATTATAGAAGAGACTGGAGTAGATGCAATTGACACACAAGAGGATGGGATAGTCAAAATTACTGCCAGGGATTTATTGAGTTTGGAAAAGTCTAAAGCTATTATTACTAATTTGACCATGGTTCCAACTGTTGGTGATATCTACAGGAATTGTGAAATCAAAACAATAGCTCCATATGGAGTATTTGTGGAAATAGCACCTGGGCGAGAGGGACTTTGCCATATTAGTGAGTTAAGTTCAGATTGGCTGGCCAAGGCAGAAGATGCTTTTAAGGTGGGAGATTATGTTGATGTTAAACTGATTGAGGTAAACGAAAAGGGGCAACTTCGCCTCAGCCGCCGAGCATTACTTCCTGATGCAGATCATGAGAATTCTGATTTAAGGCAGCCTACAGATGACTCCAAAGATGTACCTTCATCAGACAAGAACAAAGTCAAAGACCTATCTGAAACAAAGGTTTTCAGGATTTTTACTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

868

Amino Acids

94.95

Weight (kDa)

5.69

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 88 - 218 3.7e-24 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 223 - 284 4.2e-14 3' exoribonuclease family, domain 2
RNase_PH PF01138 446 - 580 7.2e-29 3' exoribonuclease family, domain 1
KH_1 PF00013 687 - 743 5.1e-06 KH domain
S1 PF00575 760 - 822 4.4e-11 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1286
Acc36I ACCTGC 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 2255
AccBSI CCGCTC 1 cut(s) 1926
AccI GTMKAC 2 cut(s) 342, 1242
AccII CGCG 1 cut(s) 1210
AciI CCGC 5 cut(s) 213, 1210, 1926, 2018, 2461
AclWI GGATC 1 cut(s) 1846
AcoI YGGCCR 2 cut(s) 1975, 2373
AcsI RAATTY 4 cut(s) 64, 701, 1793, 2494
AcuI CTGAAG 3 cut(s) 366, 1863, 2007
AdeI CACNNNGTG 1 cut(s) 1439
AfaI GTAC 4 cut(s) 561, 858, 1511, 2535
AfiI CCNNNNNNNGG 3 cut(s) 20, 2024, 2255
AflIII ACRYGT 1 cut(s) 1648
AgsI TTSAA 9 cut(s) 69, 457, 535, 1133, 1261, 1607, 1686, 1870, 2005
AhlI ACTAGT 1 cut(s) 440
AjiI CACGTC 1 cut(s) 1246
AjnI CCWGG 2 cut(s) 2189, 2323
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
AleI CACNNNNGTG 1 cut(s) 1386
Alw21I GWGCWC 5 cut(s) 994, 1393, 1558, 1661, 1751
Alw26I GTCTC 3 cut(s) 272, 289, 2129
AlwI GGATC 1 cut(s) 1846
AlwNI CAGNNNCTG 2 cut(s) 323, 623
AoxI GGCC 5 cut(s) 92, 180, 512, 1975, 2373
ApeKI GCWGC 8 cut(s) 33, 123, 126, 428, 620, 754, 826, 2509
ApoI RAATTY 4 cut(s) 64, 701, 1793, 2494
Asp700I GAANNNNTTC 3 cut(s) 539, 1496, 2032
AspLEI GCGC 3 cut(s) 33, 1287, 1293
AspS9I GGNCC 2 cut(s) 93, 132
AsuHPI GGTGA 7 cut(s) 269, 986, 1181, 1196, 1451, 1933, 2270
AsuNHI GCTAGC 1 cut(s) 757
AvaII GGWCC 1 cut(s) 132
AxyI CCTNAGG 1 cut(s) 813
BaeGI GKGCMC 2 cut(s) 113, 1381
BaeI ACNNNNGTAYC 2 cut(s) 1083, 1116
BalI TGGCCA 1 cut(s) 2375
BanII GRGCYC 1 cut(s) 994
BauI CACGAG 1 cut(s) 482
Bbv12I GWGCWC 5 cut(s) 994, 1393, 1558, 1661, 1751
BbvCI CCTCAGC 1 cut(s) 2456
BbvI GCAGC 8 cut(s) 20, 135, 138, 440, 632, 741, 813, 2521
BccI CCATC 9 cut(s) 320, 724, 1160, 1313, 1444, 1799, 1903, 1967, 2162
BceAI ACGGC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 2 cut(s) 2191, 2325
BciVI GTATCC 1 cut(s) 1084
BcoDI GTCTC 3 cut(s) 272, 289, 2129
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 6 cut(s) 15, 441, 758, 1017, 1560, 1781
BfmI CTRYAG 7 cut(s) 666, 995, 1721, 1752, 1961, 2266, 2514
BfuAI ACCTGC 1 cut(s) 422
BfuI GTATCC 1 cut(s) 1084
BglII AGATCT 1 cut(s) 1598
BlpI GCTNAGC 1 cut(s) 1714
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 2191, 2325
Bme18I GGWCC 1 cut(s) 132
BmgBI CACGTC 1 cut(s) 1246
BmgT120I GGNCC 2 cut(s) 93, 132
BmiI GGNNCC 2 cut(s) 651, 2247
BmrFI CCNGG 2 cut(s) 2191, 2325
BmtI GCTAGC 1 cut(s) 761
BpmI CTGGAG 3 cut(s) 1490, 1752, 2160
Bpu10I CCTNAGC 2 cut(s) 942, 2456
Bpu1102I GCTNAGC 1 cut(s) 1714
BpuEI CTTGAG 2 cut(s) 540, 1339
BsaAI YACGTR 2 cut(s) 1649, 2059
BsaBI GATNNNNATC 1 cut(s) 2484
BsaJI CCNNGG 7 cut(s) 645, 1373, 1402, 2190, 2241, 2324, 2376
BsaXI ACNNNNNCTCC 6 cut(s) 2278, 2295, 2308, 2325, 2394, 2424
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 2024, 2255
Bse118I RCCGGY 1 cut(s) 971
Bse1I ACTGG 5 cut(s) 303, 1507, 1840, 1940, 2143
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 2 cut(s) 1681, 1773
Bse8I GATNNNNATC 1 cut(s) 2484
BseBI CCWGG 2 cut(s) 2191, 2325
BseDI CCNNGG 7 cut(s) 645, 1373, 1402, 2190, 2241, 2324, 2376
BseGI GGATG 7 cut(s) 856, 1153, 1171, 1271, 1732, 1810, 2173
BseJI GATNNNNATC 1 cut(s) 2484
BseLI CCNNNNNNNGG 3 cut(s) 20, 2024, 2255
BseMI GCAATG 2 cut(s) 1681, 1773
BseMII CTCAG 9 cut(s) 566, 732, 804, 933, 1227, 1469, 1584, 1779, 2470
BseNI ACTGG 5 cut(s) 303, 1507, 1840, 1940, 2143
BseRI GAGGAG 2 cut(s) 146, 1803
BseSI GKGCMC 2 cut(s) 113, 1381
BseXI GCAGC 8 cut(s) 20, 135, 138, 440, 632, 741, 813, 2521
BsgI GTGCAG 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 1210
BshFI GGCC 5 cut(s) 94, 182, 514, 1977, 2375
BsiHKAI GWGCWC 5 cut(s) 994, 1393, 1558, 1661, 1751
BsiSI CCGG 1 cut(s) 972
BslFI GGGAC 3 cut(s) 136, 710, 2349
BslI CCNNNNNNNGG 3 cut(s) 20, 2024, 2255
BsmAI GTCTC 3 cut(s) 272, 289, 2129
BsmFI GGGAC 3 cut(s) 136, 710, 2349
BsnI GGCC 5 cut(s) 94, 182, 514, 1977, 2375
Bsp1286I GDGCHC 7 cut(s) 113, 994, 1381, 1393, 1558, 1661, 1751
Bsp143I GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
Bsp1720I GCTNAGC 1 cut(s) 1714
Bsp19I CCATGG 1 cut(s) 2241
BspACI CCGC 5 cut(s) 213, 1210, 1926, 2018, 2461
BspANI GGCC 5 cut(s) 94, 182, 514, 1977, 2375
BspCNI CTCAG 9 cut(s) 567, 733, 805, 934, 1228, 1468, 1585, 1780, 2469
BspFNI CGCG 1 cut(s) 1210
BspHI TCATGA 1 cut(s) 2488
BspLI GGNNCC 2 cut(s) 651, 2247
BspMAI CTGCAG 2 cut(s) 999, 1965
BspMI ACCTGC 1 cut(s) 422
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 1 cut(s) 1846
BspQI GCTCTTC 1 cut(s) 1687
BsrBI CCGCTC 1 cut(s) 1926
BsrDI GCAATG 2 cut(s) 1681, 1773
BsrFI RCCGGY 1 cut(s) 971
BsrI ACTGG 5 cut(s) 303, 1507, 1840, 1940, 2143
BssAI RCCGGY 1 cut(s) 971
BssECI CCNNGG 7 cut(s) 645, 1373, 1402, 2190, 2241, 2324, 2376
BssMI GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
BssNAI GTATAC 1 cut(s) 343
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 3 cut(s) 1373, 2241, 2376
Bst1107I GTATAC 1 cut(s) 343
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 2191, 2325
Bst4CI ACNGT 6 cut(s) 319, 587, 1096, 1416, 2001, 2254
Bst6I CTCTTC 4 cut(s) 1329, 1561, 1687, 2127
BstAPI GCANNNNNTGC 1 cut(s) 2387
BstBAI YACGTR 2 cut(s) 1649, 2059
BstC8I GCNNGC 7 cut(s) 759, 763, 831, 1105, 1289, 1965, 2373
BstDSI CCRYGG 2 cut(s) 1402, 2241
BstF5I GGATG 7 cut(s) 856, 1153, 1171, 1271, 1732, 1810, 2173
BstFNI CGCG 1 cut(s) 1210
BstHHI GCGC 3 cut(s) 33, 1287, 1293
BstKTI GATC 6 cut(s) 276, 481, 927, 1601, 1841, 2488
BstMAI GTCTC 3 cut(s) 272, 289, 2129
BstMBI GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
BstMWI GCNNNNNNNGC 9 cut(s) 30, 123, 617, 629, 860, 1003, 1207, 2387, 2460
BstNI CCWGG 2 cut(s) 2191, 2325
BstSCI CCNGG 2 cut(s) 2189, 2323
BstSFI CTRYAG 7 cut(s) 666, 995, 1721, 1752, 1961, 2266, 2514
BstSLI GKGCMC 2 cut(s) 113, 1381
BstUI CGCG 1 cut(s) 1210
BstV1I GCAGC 8 cut(s) 20, 135, 138, 440, 632, 741, 813, 2521
BstX2I RGATCY 2 cut(s) 1598, 1838
BstYI RGATCY 2 cut(s) 1598, 1838
BstZ17I GTATAC 1 cut(s) 343
Bsu36I CCTNAGG 1 cut(s) 813
BsuI GTATCC 1 cut(s) 1084
BsuRI GGCC 5 cut(s) 94, 182, 514, 1977, 2375
BtgI CCRYGG 2 cut(s) 1402, 2241
BtrI CACGTC 1 cut(s) 1246
BtsCI GGATG 7 cut(s) 856, 1153, 1171, 1271, 1732, 1810, 2173
BtsI GCAGTG 4 cut(s) 25, 36, 207, 1114
BtsIMutI CAGTG 6 cut(s) 25, 36, 207, 310, 321, 1114
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 7 cut(s) 759, 763, 831, 1105, 1289, 1965, 2373
CaiI CAGNNNCTG 2 cut(s) 323, 623
CciI TCATGA 1 cut(s) 2488
CfoI GCGC 3 cut(s) 33, 1287, 1293
Cfr10I RCCGGY 1 cut(s) 971
Cfr13I GGNCC 2 cut(s) 93, 132
CseI GACGC 1 cut(s) 509
Csp6I GTAC 4 cut(s) 560, 857, 1510, 2534
CviAII CATG 8 cut(s) 548, 956, 1474, 1527, 1580, 1889, 2242, 2489
CviQI GTAC 4 cut(s) 560, 857, 1510, 2534
DpnI GATC 6 cut(s) 275, 480, 926, 1600, 1840, 2487
DpnII GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
DraIII CACNNNGTG 1 cut(s) 1439
EaeI YGGCCR 2 cut(s) 1975, 2373
Eam1104I CTCTTC 4 cut(s) 1329, 1561, 1687, 2127
EarI CTCTTC 4 cut(s) 1329, 1561, 1687, 2127
EciI GGCGGA 1 cut(s) 2007
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 3 cut(s) 1373, 2241, 2376
Eco147I AGGCCT 2 cut(s) 182, 514
Eco24I GRGCYC 1 cut(s) 994
Eco32I GATATC 1 cut(s) 2263
Eco47I GGWCC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 3 cut(s) 366, 1863, 2007
Eco81I CCTNAGG 1 cut(s) 813
EcoICRI GAGCTC 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 2494
EcoRII CCWGG 2 cut(s) 2189, 2323
EcoRV GATATC 1 cut(s) 2263
EcoT14I CCWWGG 3 cut(s) 1373, 2241, 2376
EcoT22I ATGCAT 1 cut(s) 1852
EcoT38I GRGCYC 1 cut(s) 994
ErhI CCWWGG 3 cut(s) 1373, 2241, 2376
FaeI CATG 8 cut(s) 551, 959, 1477, 1530, 1583, 1892, 2245, 2492
FalI AAGNNNNNCTT 2 cut(s) 364, 396
FaqI GGGAC 3 cut(s) 136, 710, 2349
FatI CATG 8 cut(s) 547, 955, 1473, 1526, 1579, 1888, 2241, 2488
FauI CCCGC 1 cut(s) 1919
FauNDI CATATG 1 cut(s) 2299
FblI GTMKAC 2 cut(s) 342, 1242
FokI GGATG 7 cut(s) 863, 1160, 1178, 1258, 1739, 1817, 2180
FriOI GRGCYC 1 cut(s) 994
FspBI CTAG 6 cut(s) 15, 441, 758, 1017, 1560, 1781
FspI TGCGCA 1 cut(s) 1286
GlaI GCGC 3 cut(s) 32, 1286, 1292
GsuI CTGGAG 3 cut(s) 1490, 1752, 2160
HaeIII GGCC 5 cut(s) 94, 182, 514, 1977, 2375
HapII CCGG 1 cut(s) 972
HgaI GACGC 1 cut(s) 509
HhaI GCGC 3 cut(s) 33, 1287, 1293
Hin1II CATG 8 cut(s) 551, 959, 1477, 1530, 1583, 1892, 2245, 2492
Hin6I GCGC 3 cut(s) 31, 1285, 1291
HinP1I GCGC 3 cut(s) 31, 1285, 1291
HinfI GANTC 6 cut(s) 172, 602, 712, 737, 1012, 2522
HpaII CCGG 1 cut(s) 972
HphI GGTGA 7 cut(s) 269, 986, 1181, 1196, 1451, 1933, 2270
Hpy166II GTNNAC 7 cut(s) 343, 377, 1243, 1364, 1512, 2086, 2434
Hpy8I GTNNAC 7 cut(s) 343, 377, 1243, 1364, 1512, 2086, 2434
HpyCH4III ACNGT 6 cut(s) 319, 587, 1096, 1416, 2001, 2254
HpyCH4IV ACGT 4 cut(s) 1245, 1342, 1648, 2058
HpyF10VI GCNNNNNNNGC 9 cut(s) 30, 123, 617, 629, 860, 1003, 1207, 2387, 2460
HpySE526I ACGT 4 cut(s) 1245, 1342, 1648, 2058
Hsp92II CATG 8 cut(s) 551, 959, 1477, 1530, 1583, 1892, 2245, 2492
HspAI GCGC 3 cut(s) 31, 1285, 1291
Kzo9I GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
LguI GCTCTTC 1 cut(s) 1687
LmnI GCTCC 3 cut(s) 1553, 2015, 2299
Lsp1109I GCAGC 8 cut(s) 20, 135, 138, 440, 632, 741, 813, 2521
MaeI CTAG 6 cut(s) 15, 441, 758, 1017, 1560, 1781
MaeII ACGT 4 cut(s) 1245, 1342, 1648, 2058
MaeIII GTNAC 6 cut(s) 313, 319, 613, 1267, 1429, 1894
MalI GATC 6 cut(s) 275, 480, 926, 1600, 1840, 2487
MbiI CCGCTC 1 cut(s) 1926
MboI GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
MfeI CAATTG 1 cut(s) 2151
MflI RGATCY 2 cut(s) 1598, 1838
MhlI GDGCHC 7 cut(s) 113, 994, 1381, 1393, 1558, 1661, 1751
MlsI TGGCCA 1 cut(s) 2375
MluNI TGGCCA 1 cut(s) 2375
MlyI GAGTC 2 cut(s) 1006, 2516
MmeI TCCRAC 6 cut(s) 548, 726, 740, 1522, 1738, 2273
Mox20I TGGCCA 1 cut(s) 2375
Mph1103I ATGCAT 1 cut(s) 1852
MroXI GAANNNNTTC 3 cut(s) 539, 1496, 2032
MscI TGGCCA 1 cut(s) 2375
MslI CAYNNNNRTG 3 cut(s) 1386, 1699, 2349
Msp20I TGGCCA 1 cut(s) 2375
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 1 cut(s) 972
MspR9I CCNGG 2 cut(s) 2191, 2325
MunI CAATTG 1 cut(s) 2151
MvaI CCWGG 2 cut(s) 2191, 2325
MvnI CGCG 1 cut(s) 1210
MwoI GCNNNNNNNGC 9 cut(s) 30, 123, 617, 629, 860, 1003, 1207, 2387, 2460
NcoI CCATGG 1 cut(s) 2241
NdeI CATATG 1 cut(s) 2299
NdeII GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 8 cut(s) 551, 959, 1477, 1530, 1583, 1892, 2245, 2492
NlaIV GGNNCC 2 cut(s) 651, 2247
NmeAIII GCCGAG 1 cut(s) 2489
NmuCI GTSAC 2 cut(s) 319, 613
NsbI TGCGCA 1 cut(s) 1286
NsiI ATGCAT 1 cut(s) 1852
OliI CACNNNNGTG 1 cut(s) 1386
PagI TCATGA 1 cut(s) 2488
PceI AGGCCT 2 cut(s) 182, 514
PciSI GCTCTTC 1 cut(s) 1687
PdmI GAANNNNTTC 3 cut(s) 539, 1496, 2032
PfeI GAWTC 4 cut(s) 172, 602, 712, 737
PflMI CCANNNNNTGG 1 cut(s) 2255
PleI GAGTC 2 cut(s) 1006, 2516
PpsI GAGTC 2 cut(s) 1006, 2516
Ppu21I YACGTR 2 cut(s) 1649, 2059
Psp124BI GAGCTC 1 cut(s) 994
Psp6I CCWGG 2 cut(s) 2189, 2323
PspGI CCWGG 2 cut(s) 2189, 2323
PspN4I GGNNCC 2 cut(s) 651, 2247
PspPI GGNCC 2 cut(s) 93, 132
PstI CTGCAG 2 cut(s) 999, 1965
PstNI CAGNNNCTG 2 cut(s) 323, 623
PsuI RGATCY 2 cut(s) 1598, 1838
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 4 cut(s) 561, 858, 1511, 2535
RsaNI GTAC 4 cut(s) 560, 857, 1510, 2534
RseI CAYNNNNRTG 3 cut(s) 1386, 1699, 2349
SacI GAGCTC 1 cut(s) 994
SapI GCTCTTC 1 cut(s) 1687
Sau3AI GATC 6 cut(s) 273, 478, 924, 1598, 1838, 2485
Sau96I GGNCC 2 cut(s) 93, 132
SbfI CCTGCAGG 1 cut(s) 1965
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 2 cut(s) 1006, 2516
ScrFI CCNGG 2 cut(s) 2191, 2325
SdaI CCTGCAGG 1 cut(s) 1965
SduI GDGCHC 7 cut(s) 113, 994, 1381, 1393, 1558, 1661, 1751
SfcI CTRYAG 7 cut(s) 666, 995, 1721, 1752, 1961, 2266, 2514
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 3 cut(s) 1386, 1699, 2349
SmlI CTYRAG 2 cut(s) 555, 1354
SmoI CTYRAG 2 cut(s) 555, 1354
SpeI ACTAGT 1 cut(s) 440
Sse8387I CCTGCAGG 1 cut(s) 1965
SseBI AGGCCT 2 cut(s) 182, 514
SsiI CCGC 5 cut(s) 213, 1210, 1926, 2018, 2461
SspI AATATT 3 cut(s) 780, 1280, 1614
SspMI CTAG 6 cut(s) 15, 441, 758, 1017, 1560, 1781
SstI GAGCTC 1 cut(s) 994
StuI AGGCCT 2 cut(s) 182, 514
StyD4I CCNGG 2 cut(s) 2189, 2323
StyI CCWWGG 3 cut(s) 1373, 2241, 2376
TaaI ACNGT 6 cut(s) 319, 587, 1096, 1416, 2001, 2254
TaiI ACGT 4 cut(s) 1248, 1345, 1651, 2061
TaqI TCGA 1 cut(s) 276
TatI WGTACW 2 cut(s) 559, 1509
TauI GCSGC 2 cut(s) 215, 2463
TfiI GAWTC 4 cut(s) 172, 602, 712, 737
TscAI CASTG 6 cut(s) 25, 43, 214, 310, 328, 1114
TseFI GTSAC 2 cut(s) 319, 613
TseI GCWGC 8 cut(s) 33, 123, 126, 428, 620, 754, 826, 2509
Tsp45I GTSAC 2 cut(s) 319, 613
TspGWI ACGGA 7 cut(s) 218, 229, 301, 391, 1398, 1561, 2009
TspRI CASTG 6 cut(s) 25, 43, 214, 310, 328, 1114
Van91I CCANNNNNTGG 1 cut(s) 2255
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 4 cut(s) 64, 701, 1793, 2494
XbaI TCTAGA 1 cut(s) 1780
XmiI GTMKAC 2 cut(s) 342, 1242
XmnI GAANNNNTTC 3 cut(s) 539, 1496, 2032
XspI CTAG 6 cut(s) 15, 441, 758, 1017, 1560, 1781
ZrmI AGTACT 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 1852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.