MD12G1011400.v1.1

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009860
Physical Location & Seq
Reverse (-)
1159542 .. 1171679
12138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1011400.v1.1.491

Sequence Viewer

Length: 2751 bp
ATGTTGGCAAACCCTAACAGTTGCAGGCTCCACTGCCCCAATCCTTCCCAATTCCTGCATCCAAACAGGCCCAAATGTTTCCTCATCCCAAACTGCCCTCGTTCCCCCTGCTTCTCCAGCAAGTCCAAGTTCTGCAATTTGTCCCTGCTTCTGCCCAGAAAGACCAGCAAGAAGGGGCTTGGCATCAGAGCTTTGTCCGAGCCCGGCGGTGTTCCGTCGAGCGGTCCTCAGGGCTACCCTCAGCCGTACTCTGTCAAAATCCCTGTTGGTGATAGACATATTTTGGTTGAGACAGGTCATATTGGGAGACAAGCCAGTGCTTCTGTTACAGTGACAGATGGAGAAACCATTGTTTATACATCTGTTTGTCTGGCCGACACTCCAAGTGAGCCTTCGGACTTCTTCCCTCTTTCGGTACATTATCAGGAGCGCTTTTCAGCAGCAGGTCGAACTAGTGGAGGCTTTTTCAAACGAGAAGGAAGGGCGAAAGATCATGAGGTTCTCATATGTAGATTGATTGATAGGCCTTTGCGTCCAACTATGTTGAATGGCTTCTACCATGAAACTCAGATACTCTCTTGGGTTCTGAGTTATGATGGTTTGCATTCTCCTGATTCTTTGGCAGTCACAGCAGCTGGGATAGCAGTGGCTCTTTCAGAAGTTCCAAACTCAAAAACCGTAGCAGGAGTGCGAATTGGTCTTCTCGGTGACAAATTTATCGTGAATCCAACAACAAAGGAGATGGAAGACTCCGAGTTGGACTTGTTGGTTGCAGGCACAGATACTGCAATACTGATGATAGAGGGCTATTGTGATTTTCTCACAGAGGAAAAGTTGCTGCAAGCAGTAGAAGTTGGGCAGGATGCCATACGAGCAATTTGCAATGAGGTAGAAGCTTTGGTTAAGAAGTGTGGAAAGCCAAAAATGCTTGATGCAATCAAATTACCTCCCCCTGAGCTATATAATCATGTTGAGGAAATTGCCGGTGATGAGTTGGTAAGAGCTCTGCAGATTAAGGGAAAGATTCCTAGAAGGAAAGCTCTCTTGTCATTGGAAGGAGAAGTTTTATCTATACTTACAGAAAAGGGATATGTTAGTAAGGATGCAGCTCCCGGAGGCAGTGAAACGATACTGGATATATTTGAAGATGAAGAAGAGGATGAGGAAGTGGTTGTGGATGGTGAAGTAGATGAGGGTGATGTTCATATAAAGCCTAGTTCACGAAAGTCAACCCCCTTGTTCTTTTCTGAGGTAGATGTGAAGCTGGTATTCAAAGAAGTTACATCCAAATTTTTGCGAAGACGCATTGTGGAGGGTGGTAGAAGAAGTGATGGACGTACCCCAGATGAGATACGTCCAATTAATTCAAAATGTGGACTGCTTCCAAGGGCACATGGAAGTGCTCTTTTCACACGTGGGGAAACACAGTCATTGGCTGTTGTTACACTCGGGGATAAACAGATGGCACAGAGGTTAGACAACCTAGTAGGTGATGACGAATGGAAGAGATTCTATCTCCAGTACACATTTCCCCCTTCATGTGTTGGGGAAGTTGGACGGACTGGAGCCCCTAGTAGAAGAGAAATTGGCCATGGAATGCTTGCTGAGAGAGCTCTTGAACCAATTTTGCCTTCCGAAGATGATTTTCCTTACACTATACGTGTTGAGAGTACAATTACTGAAAGCAATGGCTCTTCAAGTATGGCATCTGTTTGTGGGGGCTGCTTAGCTTTGCAGGATGCTGGGGTTCCAATCAAGTCCTCTATAGTTGGGATAGCAATGGGTATGGTTTTGGAAACTGAGGAATTTGGGGGAAATGGTGCTCCACTAATTCTGTCTGACATAACTGGATCTGAAGATGCATCCGGGGATATGGATTTCAAGGTTGCTGGAAATGAAGATGGTGTAACTGCATTTCAGATGGACATAAAGGTGAGCGGGATTACATTACCAGTTATGAGAGAGGCACTCCTACAGGCAAAGGATGGCCGGAAGCATATTATTGCGGAAATGTTGAAGTGCTCACCTCCTCCTTCAAGATCGCTTTCTATGTATGCACCACTGATTCACATAATGAAGGTCAACCCTGAGAAAGTAAACTTAATCATTGGTAGTGGTGGGAAGAAGGTGAAGAGTATTATAGAAGAGAGTGGAGTAGACGCAATTGACACACAAGATGACGGGATTGTGAAAATTACTGGCAGGGATTTGTCTAGCATAGAAAAGTCTAAAGCTATTATTACTAACTTGACAATGGTTCCGTCAGTTGGCGATATCTTCAGGAACTGCGAGATCAAATCAATAGTTCCATATGGAGTATTCGTGGAAATAGCACCTGGGCGAGAGGGACTTTGCCATATTAGCGAGTTGACTTCGGAGTGGCTGGCCAAGGCAGAAGATGCTGTTAAAGTTGGAGATCAAATTGATGTTAAACTTATTGAGGTAAATGAGAAGGGGCAACTTCGCCTTAGCCGTAAAGCTCTACTTCCAGATACAGGTGCAGAGAATGCCAGTGCAAAGCAGTCTACAGCTGACTCGAATGATGTAGCTTCTCAAGAATCATCTGACAAGGGTTCTCCAAAGAGGGAAGAGCTCCCGCAAAAGAAGTTTATTCGGAGATTAGTCAGCACTGCCAAAGATAGACCAAATATCAGCAAAGACAAGACAAAGAAGAGCAACAGTAAAGTAGTCGGTAGCGTCTCCAGCACGGACGAAAATACTTTAGTGAATGGAGAGGCTAACATCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

917

Amino Acids

99.2

Weight (kDa)

5.55

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 92 - 222 6.3e-24 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 226 - 288 2.5e-14 3' exoribonuclease family, domain 2
RNase_PH PF01138 450 - 584 3.2e-29 3' exoribonuclease family, domain 1
KH_1 PF00013 691 - 748 1.5e-07 KH domain
S1 PF00575 764 - 826 6.1e-12 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 434
AccBSI CCGCTC 2 cut(s) 222, 1938
AccI GTMKAC 2 cut(s) 2157, 2525
AciI CCGC 5 cut(s) 207, 222, 1938, 2006, 2597
AclWI GGATC 1 cut(s) 1858
AcoI YGGCCR 4 cut(s) 372, 1588, 1987, 2385
AcsI RAATTY 3 cut(s) 713, 1289, 1805
AcuI CTGAAG 2 cut(s) 1875, 2263
AcvI CACGTG 1 cut(s) 1415
AfaI GTAC 5 cut(s) 248, 417, 1339, 1523, 1672
AfeI AGCGCT 1 cut(s) 431
AfiI CCNNNNNNNGG 4 cut(s) 221, 412, 2267, 2495
AflIII ACRYGT 2 cut(s) 1412, 1660
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 1 cut(s) 2335
AjuI GAANNNNNNNTTGG 4 cut(s) 376, 408, 1615, 1647
Alw21I GWGCWC 6 cut(s) 1006, 1405, 1615, 1825, 2024, 2595
Alw26I GTCTC 3 cut(s) 284, 301, 2704
AlwI GGATC 1 cut(s) 1858
AlwNI CAGNNNCTG 3 cut(s) 635, 785, 2286
Ama87I CYCGRG 1 cut(s) 1448
Aor51HI AGCGCT 1 cut(s) 431
AoxI GGCC 6 cut(s) 68, 372, 524, 1588, 1987, 2385
ApeKI GCWGC 5 cut(s) 440, 632, 838, 1106, 1722
ApoI RAATTY 3 cut(s) 713, 1289, 1805
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 2 cut(s) 551, 1508
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 2 cut(s) 69, 224
AsuC2I CCSGG 3 cut(s) 204, 1113, 1867
AsuHPI GGTGA 9 cut(s) 281, 719, 998, 1193, 1208, 1502, 1945, 2016, 2140
AvaI CYCGRG 1 cut(s) 1448
AvaII GGWCC 1 cut(s) 224
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 1393
BalI TGGCCA 2 cut(s) 1590, 2387
BanII GRGCYC 5 cut(s) 204, 1006, 1570, 1615, 2595
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 3 cut(s) 692, 753, 1306
Bbv12I GWGCWC 6 cut(s) 1006, 1405, 1615, 1825, 2024, 2595
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 5 cut(s) 452, 644, 825, 1118, 1709
BccI CCATC 9 cut(s) 332, 590, 736, 1172, 1325, 1456, 1895, 1915, 1979
BceAI ACGGC 2 cut(s) 229, 2457
BcgI CGANNNNNNTGC 2 cut(s) 861, 895
BciT130I CCWGG 1 cut(s) 2337
BcnI CCSGG 3 cut(s) 204, 1113, 1867
BcoDI GTCTC 3 cut(s) 284, 301, 2704
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 6 cut(s) 453, 1029, 1215, 1484, 1572, 2214
BfmI CTRYAG 4 cut(s) 1007, 1764, 1973, 2526
BfoI RGCGCY 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 5 cut(s) 441, 633, 839, 1107, 1723
BlpI GCTNAGC 1 cut(s) 1726
BlsI GCNGC 5 cut(s) 442, 634, 840, 1108, 1724
Bme1390I CCNGG 4 cut(s) 204, 1113, 1867, 2337
Bme18I GGWCC 1 cut(s) 224
BmeT110I CYCGRG 1 cut(s) 1448
BmgT120I GGNCC 2 cut(s) 69, 224
BmiI GGNNCC 4 cut(s) 29, 1567, 1749, 2259
BmrFI CCNGG 4 cut(s) 204, 1113, 1867, 2337
BpiI GAAGAC 3 cut(s) 692, 753, 1306
BplI GAGNNNNNCTC 4 cut(s) 211, 243, 1953, 1985
BpmI CTGGAG 4 cut(s) 100, 1502, 1584, 2686
Bpu10I CCTNAGC 3 cut(s) 240, 954, 2468
Bpu1102I GCTNAGC 1 cut(s) 1726
BpuEI CTTGAG 1 cut(s) 2538
BpuMI CCSGG 3 cut(s) 204, 1113, 1867
BsaAI YACGTR 2 cut(s) 1415, 1661
BsaJI CCNNGG 5 cut(s) 1385, 1591, 1866, 2336, 2388
BsaXI ACNNNNNCTCC 2 cut(s) 2307, 2337
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 412, 2267, 2495
Bse118I RCCGGY 1 cut(s) 983
Bse1I ACTGG 8 cut(s) 315, 1137, 1519, 1567, 1852, 1952, 2203, 2511
Bse21I CCTNAGG 1 cut(s) 228
Bse3DI GCAATG 3 cut(s) 889, 1693, 1785
BseBI CCWGG 1 cut(s) 2337
BseDI CCNNGG 5 cut(s) 1385, 1591, 1866, 2336, 2388
BseLI CCNNNNNNNGG 4 cut(s) 221, 412, 2267, 2495
BseMI GCAATG 3 cut(s) 889, 1693, 1785
BseMII CTCAG 9 cut(s) 242, 254, 578, 581, 945, 1239, 1596, 1791, 2079
BseNI ACTGG 8 cut(s) 315, 1137, 1519, 1567, 1852, 1952, 2203, 2511
BseRI GAGGAG 1 cut(s) 2019
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 5 cut(s) 452, 644, 825, 1118, 1709
BseYI CCCAGC 2 cut(s) 635, 1742
BsgI GTGCAG 1 cut(s) 2520
BshFI GGCC 6 cut(s) 70, 374, 526, 1590, 1989, 2387
BsiHKAI GWGCWC 6 cut(s) 1006, 1405, 1615, 1825, 2024, 2595
BsiHKCI CYCGRG 1 cut(s) 1448
BsiSI CCGG 5 cut(s) 204, 984, 1113, 1866, 1990
BslFI GGGAC 2 cut(s) 127, 2361
BslI CCNNNNNNNGG 4 cut(s) 221, 412, 2267, 2495
BsmAI GTCTC 3 cut(s) 284, 301, 2704
BsmBI CGTCTC 1 cut(s) 2704
BsmFI GGGAC 2 cut(s) 127, 2361
BsmI GAATGC 3 cut(s) 604, 1602, 2512
BsnI GGCC 6 cut(s) 70, 374, 526, 1590, 1989, 2387
BsoBI CYCGRG 1 cut(s) 1448
Bsp1286I GDGCHC 9 cut(s) 204, 1006, 1393, 1405, 1570, 1615, 1825, 2024, 2595
Bsp143I GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
Bsp1720I GCTNAGC 1 cut(s) 1726
Bsp19I CCATGG 1 cut(s) 1591
BspACI CCGC 5 cut(s) 207, 222, 1938, 2006, 2597
BspANI GGCC 6 cut(s) 70, 374, 526, 1590, 1989, 2387
BspCNI CTCAG 9 cut(s) 241, 253, 579, 580, 946, 1240, 1597, 1792, 2080
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 4 cut(s) 29, 1567, 1749, 2259
BspMAI CTGCAG 1 cut(s) 1011
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 1 cut(s) 1858
BspQI GCTCTTC 3 cut(s) 1699, 2583, 2666
BsrBI CCGCTC 2 cut(s) 222, 1938
BsrDI GCAATG 3 cut(s) 889, 1693, 1785
BsrFI RCCGGY 1 cut(s) 983
BsrI ACTGG 8 cut(s) 315, 1137, 1519, 1567, 1852, 1952, 2203, 2511
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 5 cut(s) 1385, 1591, 1866, 2336, 2388
BssMI GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
BssT1I CCWWGG 3 cut(s) 1385, 1591, 2388
Bst2UI CCWGG 1 cut(s) 2337
Bst4CI ACNGT 5 cut(s) 20, 331, 679, 1428, 2681
Bst6I CTCTTC 8 cut(s) 1149, 1499, 1573, 1699, 2126, 2139, 2583, 2666
BstAPI GCANNNNNTGC 2 cut(s) 2399, 2507
BstBAI YACGTR 2 cut(s) 1415, 1661
BstC8I GCNNGC 5 cut(s) 26, 775, 843, 1602, 2385
BstDSI CCRYGG 1 cut(s) 1591
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 5 cut(s) 493, 1853, 2042, 2295, 2419
BstMAI GTCTC 3 cut(s) 284, 301, 2704
BstMBI GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
BstNI CCWGG 1 cut(s) 2337
BstSCI CCNGG 4 cut(s) 202, 1111, 1865, 2335
BstSFI CTRYAG 4 cut(s) 1007, 1764, 1973, 2526
BstSLI GKGCMC 1 cut(s) 1393
BstV1I GCAGC 5 cut(s) 452, 644, 825, 1118, 1709
BstV2I GAAGAC 3 cut(s) 692, 753, 1306
BstX2I RGATCY 1 cut(s) 1850
BstYI RGATCY 1 cut(s) 1850
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 6 cut(s) 70, 374, 526, 1590, 1989, 2387
BtgI CCRYGG 1 cut(s) 1591
BtsI GCAGTG 4 cut(s) 31, 651, 1126, 2628
BtsIMutI CAGTG 8 cut(s) 31, 322, 336, 651, 1126, 2060, 2518, 2628
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 5 cut(s) 26, 775, 843, 1602, 2385
CaiI CAGNNNCTG 3 cut(s) 635, 785, 2286
CciI TCATGA 1 cut(s) 493
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 2 cut(s) 69, 224
CseI GACGC 4 cut(s) 521, 1311, 2168, 2686
Csp6I GTAC 5 cut(s) 247, 416, 1338, 1522, 1671
CviAII CATG 6 cut(s) 494, 560, 968, 1394, 1539, 1592
CviQI GTAC 5 cut(s) 247, 416, 1338, 1522, 1671
DpnI GATC 5 cut(s) 492, 1852, 2041, 2294, 2418
DpnII GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
EaeI YGGCCR 4 cut(s) 372, 1588, 1987, 2385
Eam1104I CTCTTC 8 cut(s) 1149, 1499, 1573, 1699, 2126, 2139, 2583, 2666
EarI CTCTTC 8 cut(s) 1149, 1499, 1573, 1699, 2126, 2139, 2583, 2666
Ecl136II GAGCTC 3 cut(s) 1004, 1613, 2593
Eco130I CCWWGG 3 cut(s) 1385, 1591, 2388
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 5 cut(s) 204, 1006, 1570, 1615, 2595
Eco32I GATATC 1 cut(s) 2275
Eco47I GGWCC 1 cut(s) 224
Eco47III AGCGCT 1 cut(s) 431
Eco53kI GAGCTC 3 cut(s) 1004, 1613, 2593
Eco57I CTGAAG 2 cut(s) 1875, 2263
Eco72I CACGTG 1 cut(s) 1415
Eco81I CCTNAGG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 1448
EcoICRI GAGCTC 3 cut(s) 1004, 1613, 2593
EcoRII CCWGG 1 cut(s) 2335
EcoRV GATATC 1 cut(s) 2275
EcoT14I CCWWGG 3 cut(s) 1385, 1591, 2388
EcoT22I ATGCAT 1 cut(s) 1864
EcoT38I GRGCYC 5 cut(s) 204, 1006, 1570, 1615, 2595
ErhI CCWWGG 3 cut(s) 1385, 1591, 2388
Esp3I CGTCTC 1 cut(s) 2704
FaeI CATG 6 cut(s) 497, 563, 971, 1397, 1542, 1595
FalI AAGNNNNNCTT 4 cut(s) 376, 408, 2469, 2501
FaqI GGGAC 2 cut(s) 127, 2361
FatI CATG 6 cut(s) 493, 559, 967, 1393, 1538, 1591
FauI CCCGC 2 cut(s) 1931, 2604
FauNDI CATATG 2 cut(s) 506, 2311
FblI GTMKAC 2 cut(s) 2157, 2525
Fnu4HI GCNGC 5 cut(s) 441, 633, 839, 1107, 1723
FriOI GRGCYC 5 cut(s) 204, 1006, 1570, 1615, 2595
Fsp4HI GCNGC 5 cut(s) 441, 633, 839, 1107, 1723
FspBI CTAG 6 cut(s) 453, 1029, 1215, 1484, 1572, 2214
GlaI GCGC 1 cut(s) 431
GluI GCNGC 5 cut(s) 441, 633, 839, 1107, 1723
GsaI CCCAGC 2 cut(s) 639, 1746
GsuI CTGGAG 4 cut(s) 100, 1502, 1584, 2686
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 6 cut(s) 70, 374, 526, 1590, 1989, 2387
HapII CCGG 5 cut(s) 204, 984, 1113, 1866, 1990
HgaI GACGC 4 cut(s) 521, 1311, 2168, 2686
HhaI GCGC 1 cut(s) 432
Hin1II CATG 6 cut(s) 497, 563, 971, 1397, 1542, 1595
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 3 cut(s) 1230, 2083, 2370
HindII GTYRAC 3 cut(s) 1230, 2083, 2370
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 8 cut(s) 614, 724, 749, 1024, 1509, 2065, 2534, 2558
HpaII CCGG 5 cut(s) 204, 984, 1113, 1866, 1990
HphI GGTGA 9 cut(s) 281, 719, 998, 1193, 1208, 1502, 1945, 2016, 2140
Hpy166II GTNNAC 9 cut(s) 1220, 1230, 1376, 1524, 2083, 2098, 2158, 2370, 2526
Hpy8I GTNNAC 9 cut(s) 1220, 1230, 1376, 1524, 2083, 2098, 2158, 2370, 2526
Hpy99I CGWCG 1 cut(s) 220
HpyCH4III ACNGT 5 cut(s) 20, 331, 679, 1428, 2681
HpyCH4IV ACGT 4 cut(s) 1336, 1354, 1414, 1660
HpySE526I ACGT 4 cut(s) 1336, 1354, 1414, 1660
Hsp92II CATG 6 cut(s) 497, 563, 971, 1397, 1542, 1595
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
LguI GCTCTTC 3 cut(s) 1699, 2583, 2666
LmnI GCTCC 6 cut(s) 33, 427, 1114, 1565, 1828, 2598
Lsp1109I GCAGC 5 cut(s) 452, 644, 825, 1118, 1709
MaeI CTAG 6 cut(s) 453, 1029, 1215, 1484, 1572, 2214
MaeII ACGT 4 cut(s) 1336, 1354, 1414, 1660
MaeIII GTNAC 7 cut(s) 325, 331, 625, 707, 1279, 1441, 1906
MalI GATC 5 cut(s) 492, 1852, 2041, 2294, 2418
MbiI CCGCTC 2 cut(s) 222, 1938
MboI GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
MfeI CAATTG 1 cut(s) 2163
MflI RGATCY 1 cut(s) 1850
MhlI GDGCHC 9 cut(s) 204, 1006, 1393, 1405, 1570, 1615, 1825, 2024, 2595
MlsI TGGCCA 2 cut(s) 1590, 2387
MluNI TGGCCA 2 cut(s) 1590, 2387
MlyI GAGTC 2 cut(s) 743, 2528
MmeI TCCRAC 5 cut(s) 560, 738, 752, 1534, 2392
Mox20I TGGCCA 2 cut(s) 1590, 2387
Mph1103I ATGCAT 1 cut(s) 1864
MroXI GAANNNNTTC 2 cut(s) 551, 1508
MscI TGGCCA 2 cut(s) 1590, 2387
MseI TTAA 6 cut(s) 903, 1014, 1362, 2102, 2406, 2430
MslI CAYNNNNRTG 2 cut(s) 1398, 1931
Msp20I TGGCCA 2 cut(s) 1590, 2387
MspA1I CMGCKG 2 cut(s) 635, 2531
MspI CCGG 5 cut(s) 204, 984, 1113, 1866, 1990
MspR9I CCNGG 4 cut(s) 204, 1113, 1867, 2337
MunI CAATTG 1 cut(s) 2163
Mva1269I GAATGC 3 cut(s) 604, 1602, 2512
MvaI CCWGG 1 cut(s) 2337
NciI CCSGG 3 cut(s) 204, 1113, 1867
NcoI CCATGG 1 cut(s) 1591
NdeI CATATG 2 cut(s) 506, 2311
NdeII GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
NlaIII CATG 6 cut(s) 497, 563, 971, 1397, 1542, 1595
NlaIV GGNNCC 4 cut(s) 29, 1567, 1749, 2259
NmuCI GTSAC 3 cut(s) 331, 625, 707
NsiI ATGCAT 1 cut(s) 1864
PagI TCATGA 1 cut(s) 493
PceI AGGCCT 1 cut(s) 526
PciSI GCTCTTC 3 cut(s) 1699, 2583, 2666
PcsI WCGNNNNNNNCGW 1 cut(s) 2470
PctI GAATGC 3 cut(s) 604, 1602, 2512
PdmI GAANNNNTTC 2 cut(s) 551, 1508
PfeI GAWTC 6 cut(s) 614, 724, 1024, 1509, 2065, 2558
PfoI TCCNGGA 1 cut(s) 1111
PkrI GCNGC 5 cut(s) 442, 634, 840, 1108, 1724
PleI GAGTC 2 cut(s) 743, 2528
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 2 cut(s) 743, 2528
Ppu21I YACGTR 2 cut(s) 1415, 1661
PshBI ATTAAT 1 cut(s) 1362
Psp124BI GAGCTC 3 cut(s) 1006, 1615, 2595
Psp6I CCWGG 1 cut(s) 2335
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 2 cut(s) 635, 1742
PspGI CCWGG 1 cut(s) 2335
PspN4I GGNNCC 4 cut(s) 29, 1567, 1749, 2259
PspPI GGNCC 2 cut(s) 69, 224
PstI CTGCAG 1 cut(s) 1011
PstNI CAGNNNCTG 3 cut(s) 635, 785, 2286
PsuI RGATCY 1 cut(s) 1850
PvuII CAGCTG 2 cut(s) 635, 2531
RsaI GTAC 5 cut(s) 248, 417, 1339, 1523, 1672
RsaNI GTAC 5 cut(s) 247, 416, 1338, 1522, 1671
RseI CAYNNNNRTG 2 cut(s) 1398, 1931
SacI GAGCTC 3 cut(s) 1006, 1615, 2595
SapI GCTCTTC 3 cut(s) 1699, 2583, 2666
SaqAI TTAA 6 cut(s) 903, 1014, 1362, 2102, 2406, 2430
SatI GCNGC 5 cut(s) 441, 633, 839, 1107, 1723
Sau3AI GATC 5 cut(s) 490, 1850, 2039, 2292, 2416
Sau96I GGNCC 2 cut(s) 69, 224
SchI GAGTC 2 cut(s) 743, 2528
ScrFI CCNGG 4 cut(s) 204, 1113, 1867, 2337
SduI GDGCHC 9 cut(s) 204, 1006, 1393, 1405, 1570, 1615, 1825, 2024, 2595
SfcI CTRYAG 4 cut(s) 1007, 1764, 1973, 2526
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 2 cut(s) 1398, 1931
SmlI CTYRAG 1 cut(s) 2553
SmoI CTYRAG 1 cut(s) 2553
SpeI ACTAGT 1 cut(s) 452
SseBI AGGCCT 1 cut(s) 526
SsiI CCGC 5 cut(s) 207, 222, 1938, 2006, 2597
SspMI CTAG 6 cut(s) 453, 1029, 1215, 1484, 1572, 2214
SstI GAGCTC 3 cut(s) 1006, 1615, 2595
StuI AGGCCT 1 cut(s) 526
StyD4I CCNGG 4 cut(s) 202, 1111, 1865, 2335
StyI CCWWGG 3 cut(s) 1385, 1591, 2388
TaaI ACNGT 5 cut(s) 20, 331, 679, 1428, 2681
TaiI ACGT 4 cut(s) 1339, 1357, 1417, 1663
TaqI TCGA 3 cut(s) 218, 448, 2537
TatI WGTACW 2 cut(s) 1521, 1670
TfiI GAWTC 6 cut(s) 614, 724, 1024, 1509, 2065, 2558
Tru1I TTAA 6 cut(s) 903, 1014, 1362, 2102, 2406, 2430
Tru9I TTAA 6 cut(s) 903, 1014, 1362, 2102, 2406, 2430
TscAI CASTG 8 cut(s) 38, 322, 336, 651, 1126, 2067, 2518, 2635
TseFI GTSAC 3 cut(s) 331, 625, 707
TseI GCWGC 5 cut(s) 440, 632, 838, 1106, 1722
Tsp45I GTSAC 3 cut(s) 331, 625, 707
TspDTI ATGAA 6 cut(s) 576, 1164, 1193, 1527, 1911, 2090
TspGWI ACGGA 4 cut(s) 204, 1573, 2250, 2723
TspRI CASTG 8 cut(s) 38, 322, 336, 651, 1126, 2067, 2518, 2635
VpaK11BI GGWCC 1 cut(s) 224
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 3 cut(s) 713, 1289, 1805
XmiI GTMKAC 2 cut(s) 2157, 2525
XmnI GAANNNNTTC 2 cut(s) 551, 1508
XspI CTAG 6 cut(s) 453, 1029, 1215, 1484, 1572, 2214
Zsp2I ATGCAT 1 cut(s) 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.