Rroxscaffold_6G00389540

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009860
Physical Location & Seq
Forward (+)
3227684 .. 3250570
22887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389540.1

Sequence Viewer

Length: 3147 bp
ATGTTGGCAAACCCTAGCAGTGGCAGGCTGCACTGCGCTAAACCCTTTCATTTCCACCCAAACCCAAAATCAAACGAGCCCAAATGTTTTGTGTTCCGAAACTGGCCCCCGCGCAGCAAGTCCAAGTCCAAGTCCAAGTCCAAGTTCTGCAGTTTGTCCCTCCTCGTTCCCAGAAAGAATCGCAAGGCCTTTGGCATCAGAGCTTTGACCGACGCCGCCGGAGCTGCGGCGGTTGATGTCACTCAGCCCTATTCCGTTAAAATCCCCGTTGGTGATAGACACATTTTGATTGAGACAGGTCATATTGGGAGACAAGCCAGTGCTTCTGTTACAGTCACAGATGGAGAAACTATTGTTTACACATCTGTTTGTTTGGCTGATACTCCAAGTGAACCGTCAGACTTTTTCCCTCTTTCCGTTCATTATCAGGAGCGTTTTTCGGCTGCAGGTCGGACTAGTGGAGGATTTTTTAAACGAGAAGGAAGGGCAAAAGATCACGAGGTTCTCATTTGCAGATTGATTGATAGGCCTTTGCGTCCAACTATGTTGAATGGCTTCTACCATGAAACTCAGATACTTTCTTGGGTGCTGAGTTATGATGGTTTGCACTCTCCTGATTCTTTGGCAGTCACAGCAGCTGGAATAGCTGTTGCTCTTTCAGAGGTTCCAAACACAAGAACTATAGCAGGAGTTAGAATTGGTCTTGTTGGTGACAAATTTATTGTGAATCCAACAACTAAGGAGATGGAAGACTCTGAGTTGGACTTGCTGCTAGCTGGCACAGATAGTGCAATATTGATGATAGAGGGTTATTGTGATTTTCTGCCCGAGGAAAAGTTGCTGCAAGCAATAGAAGTTGGGCAGGATGCTGTACGAGCAATTTGCAATGAGGTGGAGGCTTTGGTTAAGAAGTGTGGGAAGCCAAAAATGTTTGATGCAATTAAATTACCTCCCCCTGAGCTATATAATCATGTTGAGGAGCATTTAGTTTTGAACATTGGTATGTGTATAAAGGCAATTAAATTTTGGCTGGTATATTGGCCATTTCAATGGATTGAAACAGATGATTCTTTGAGGATTACAAAGGAAATTGCTGGTGATGAGTTGGTAAGAGCTCTGCAGATTACGCACAAGATTCCTAGAAGGAAAGCCCTGTTATCATTGGAAGGAGAAGTTTTAACTATACTTACTGAAAAGGGATATGTTAGTAAGGATACTGCTCTTGCAGGCAGTGAAACGGTACCAGATATATTTGAAGATGAAGATGAGGATGAGGAAGTGATTGTGGATGGTGAAGTAGATGAAGGTGATGTTCATATAAAGCCTACCTCACGGAAATCGACTCCCTTGGCGTTCAAGTTTGCAAGACGTGAAGCTGGTATTCAAAGAAGTAACATCCAAATTTTTGCGCAGGCGCATTGTGGAGACCTGGTCCAAATACATTTGGTTGCTGGAAAAAGAGAATCAACTCAACCCAATATTGTCGGGTCTGTCAGCCTGGAGAGTAAAAGGAGAAGAGATGTGGCGTGGGAGAGGGAGAGTGGCAGTGGCGGCCTCAAGAGGGAGAGGGTGGAAGAAGAAGTGATGGGCGGACCCCAGAAGGGATACGTCCAATTAACTCAAGATGTGGACTACTTCCAAGGGCACATGGAAGTGCTCTTTTCACACGAGGGGAAACACAGGTCCTTTCGCTTTTCGTCTGTTTTACAGTCATTGGCTGTGGTTACACTTGGTGATAAGCAGATGGCTCAGAGGATAGACAACCTAGTGGGTGTAGATGAATGGAAAAGATTCTATCTCCAGTACACATTTCCCCCTTCATGTGTTGGGGAGGTTGGACGGAATGGAGCACCTAGTAGAAGAGAAATTGGTCATGGAATGCTGGCTGAGAGAGCTCTTGAACCAATATTGCCTTGTGATGATGATTTTCCTTACACTATACGTGTTGAGAGCACAATTACTGAAAGCAATGGCTCTTCAAGCACTACAGGATGCTGGAGTTCCAATCAAGTGCTCTATAGTTGGAATAGCAATGGGTCTGGTTCTAGATACCGAGGAATTTGGGGGGATGGTTCTCCGCTTATTCTCTCTGACATAACTGGATCTGAAGATGCATCAGGGGATATGGATTTCAAGGTTGCTGGAAATGAACATGGTGTAACAGCATTTCAGATGGATATAAAGGTGAGTGGGATTACATTATCAGTTATGAGAAAGGCACTCCTACAAGCCAAAGATGGCCGGAAGCATATTATTGCGGAACTGTTGAAATGCTCCCCTCCACCTTCAGGAAGGCTTTCCAAGTATGCACCACTGATTCACATAATGAAGGTCAAACCTGAGAAAGTAAACTTAATCATTGGTTCTGGTGGGAAGAAGGTCAAAAGTATTATAGAAGAGACCGGAGTAGATGCAATTGACACACAAGAGGATGGGATAGTGAAAATTACTGCCAGGGATTTATTGAGTTTAGAAAAGTCTAAAGCTATTATTGCTAATTTGACAATGGTTCCAACTGTTGGTGATATCTACAGGGGGCATTTGTCTTTGCACGGGTCTGGCTTTGAGCTGCTGTTGGGCTCTGGAGCTGGGATTTCTTTGGGCTTGGCTAGGTTTAGGGTTGTCTTGGACTGCTTTATGCTTTTAGTTAGGAATTGCGAAATTAAAACAATAGCTCCATATGGAGTATTTGTGGAAATAGCACCTGGCCGAGAGGGACTTTGCCATATTAGTGAGTTAAGTTCAGATTGGCTGGCAAAGGCAGAAGATGCTTTTAAGGTGGGAGATCATGTTGATGTTAAACTTATTGAGGTAAATGAGAAGGGGCAACTTCGCCTTAGCCGCCGAGCATTACTTCCAGATGCAGATCCTGAGAATGCTGGTGTAAGGCAGCCTACAGGTGACTCCAAAGATGTACCTTCTCAAACATCGGACAAAAACAAAGTCAAAGGTACAAGCTCACCTAAAAATAATTCATCAGAAAGTACTCTGCTCCCACAAAAAAAGTTCATCCGGAGATCAGTCAGCCCTGCCAAAGATAGACAAAATATTAATAATGATGCGACAAAGAAGAGCAGCAGTAGAGTAGTGAGCAGTTCCAGCAAGGATGAAAATACTTTAGTAAATGGAGGTGCTAACATTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

1048

Amino Acids

115.0

Weight (kDa)

6.72

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 93 - 223 4.2e-24 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 228 - 289 7.1e-14 3' exoribonuclease family, domain 2
RNase_PH PF01138 572 - 665 4.5e-09 3' exoribonuclease family, domain 1
S1 PF00575 887 - 949 2.8e-11 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1410
Acc36I ACCTGC 1 cut(s) 437
Acc65I GGTACC 1 cut(s) 1240
AccB1I GGYRCC 1 cut(s) 1240
AccB7I CCANNNNNTGG 1 cut(s) 2519
AccII CGCG 1 cut(s) 112
AccIII TCCGGA 1 cut(s) 3012
AciI CCGC 9 cut(s) 110, 216, 227, 230, 1551, 1590, 2078, 2258, 2842
AclWI GGATC 2 cut(s) 2110, 2861
AcoI YGGCCR 3 cut(s) 1040, 2239, 2707
AcsI RAATTY 4 cut(s) 716, 1022, 1401, 2058
AcuI CTGAAG 2 cut(s) 2127, 2271
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 1733
AfaI GTAC 6 cut(s) 873, 1242, 1805, 2916, 2953, 2986
AfiI CCNNNNNNNGG 5 cut(s) 20, 1561, 1601, 2288, 2519
AflIII ACRYGT 1 cut(s) 1942
AhlI ACTAGT 1 cut(s) 455
AjiI CACGTC 1 cut(s) 1370
AjnI CCWGG 4 cut(s) 1428, 1497, 2453, 2704
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw21I GWGCWC 6 cut(s) 1117, 1659, 1852, 1897, 1955, 2016
Alw26I GTCTC 4 cut(s) 287, 304, 1419, 2393
AlwI GGATC 2 cut(s) 2110, 2861
AlwNI CAGNNNCTG 2 cut(s) 638, 2870
Ama87I CYCGRG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 3012
AoxI GGCC 7 cut(s) 104, 186, 527, 1040, 1552, 2239, 2707
ApoI RAATTY 4 cut(s) 716, 1022, 1401, 2058
ArsI GACNNNNNNTTYG 2 cut(s) 2928, 2960
AseI ATTAAT 1 cut(s) 3051
Asp700I GAANNNNTTC 3 cut(s) 554, 1790, 2296
Asp718I GGTACC 1 cut(s) 1240
AspLEI GCGC 4 cut(s) 38, 114, 1411, 1417
AspS9I GGNCC 4 cut(s) 105, 1432, 1592, 1683
AsuNHI GCTAGC 1 cut(s) 772
AvaI CYCGRG 1 cut(s) 827
AvaII GGWCC 3 cut(s) 1432, 1592, 1683
BaeGI GKGCMC 1 cut(s) 1647
BalI TGGCCA 1 cut(s) 1042
BanI GGYRCC 1 cut(s) 1240
BanII GRGCYC 4 cut(s) 81, 1117, 1897, 2582
BauI CACGAG 2 cut(s) 497, 1667
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 6 cut(s) 1117, 1659, 1852, 1897, 1955, 2016
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciT130I CCWGG 4 cut(s) 1430, 1499, 2455, 2706
BciVI GTATCC 2 cut(s) 1207, 1598
BcoDI GTCTC 4 cut(s) 287, 304, 1419, 2393
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 8 cut(s) 15, 456, 773, 1140, 1766, 1854, 2046, 2610
BfmI CTRYAG 8 cut(s) 148, 444, 681, 1118, 1986, 2017, 2530, 2895
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 2 cut(s) 1207, 1598
BmcAI AGTACT 1 cut(s) 2986
Bme1390I CCNGG 4 cut(s) 1430, 1499, 2455, 2706
Bme18I GGWCC 3 cut(s) 1432, 1592, 1683
BmeT110I CYCGRG 1 cut(s) 827
BmgBI CACGTC 1 cut(s) 1370
BmgT120I GGNCC 4 cut(s) 105, 1432, 1592, 1683
BmiI GGNNCC 5 cut(s) 107, 666, 1242, 1594, 2511
BmrFI CCNGG 4 cut(s) 1430, 1499, 2455, 2706
BmtI GCTAGC 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 4 cut(s) 1520, 1784, 2017, 2604
Bpu10I CCTNAGC 2 cut(s) 957, 2837
BpuEI CTTGAG 2 cut(s) 1541, 1605
BsaAI YACGTR 1 cut(s) 1943
BsaBI GATNNNNATC 1 cut(s) 2865
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1419, 2393
BsaJI CCNNGG 5 cut(s) 828, 1347, 1639, 2053, 2454
BsaWI WCCGGW 2 cut(s) 2402, 3012
BsaXI ACNNNNNCTCC 8 cut(s) 1416, 1446, 2659, 2676, 2689, 2706, 2775, 2805
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 1561, 1601, 2288, 2519
Bse1I ACTGG 4 cut(s) 107, 318, 1801, 2104
Bse3DI GCAATG 3 cut(s) 892, 1975, 2038
Bse8I GATNNNNATC 1 cut(s) 2865
BseAI TCCGGA 1 cut(s) 3012
BseBI CCWGG 4 cut(s) 1430, 1499, 2455, 2706
BseDI CCNNGG 5 cut(s) 828, 1347, 1639, 2053, 2454
BseGI GGATG 9 cut(s) 871, 1276, 1294, 1395, 1997, 2074, 2437, 3009, 3112
BseJI GATNNNNATC 1 cut(s) 2865
BseLI CCNNNNNNNGG 5 cut(s) 20, 1561, 1601, 2288, 2519
BseMI GCAATG 3 cut(s) 892, 1975, 2038
BseMII CTCAG 9 cut(s) 257, 581, 584, 747, 948, 1763, 1878, 2331, 2862
BseNI ACTGG 4 cut(s) 107, 318, 1801, 2104
BseRI GAGGAG 2 cut(s) 152, 992
BseSI GKGCMC 1 cut(s) 1647
BseYI CCCAGC 1 cut(s) 2588
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 7 cut(s) 106, 188, 529, 1042, 1554, 2241, 2709
BshNI GGYRCC 1 cut(s) 1240
BsiHKAI GWGCWC 6 cut(s) 1117, 1659, 1852, 1897, 1955, 2016
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 4 cut(s) 219, 2242, 2403, 3013
BslFI GGGAC 2 cut(s) 142, 2730
BslI CCNNNNNNNGG 5 cut(s) 20, 1561, 1601, 2288, 2519
BsmAI GTCTC 4 cut(s) 287, 304, 1419, 2393
BsmFI GGGAC 2 cut(s) 142, 2730
BsmI GAATGC 2 cut(s) 1884, 2881
BsnI GGCC 7 cut(s) 106, 188, 529, 1042, 1554, 2241, 2709
Bso31I GGTCTC 2 cut(s) 1419, 2393
BsoBI CYCGRG 1 cut(s) 827
Bsp1286I GDGCHC 9 cut(s) 81, 1117, 1647, 1659, 1852, 1897, 1955, 2016, 2582
Bsp13I TCCGGA 1 cut(s) 3012
Bsp143I GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
BspACI CCGC 9 cut(s) 110, 216, 227, 230, 1551, 1590, 2078, 2258, 2842
BspANI GGCC 7 cut(s) 106, 188, 529, 1042, 1554, 2241, 2709
BspCNI CTCAG 9 cut(s) 256, 582, 583, 748, 949, 1762, 1879, 2332, 2863
BspEI TCCGGA 1 cut(s) 3012
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 5 cut(s) 107, 666, 1242, 1594, 2511
BspMAI CTGCAG 3 cut(s) 152, 448, 1122
BspMI ACCTGC 1 cut(s) 437
BspOI GCTAGC 1 cut(s) 776
BspPI GGATC 2 cut(s) 2110, 2861
BspQI GCTCTTC 2 cut(s) 1981, 3065
BspT107I GGYRCC 1 cut(s) 1240
BspTNI GGTCTC 2 cut(s) 1419, 2393
BsrDI GCAATG 3 cut(s) 892, 1975, 2038
BsrI ACTGG 4 cut(s) 107, 318, 1801, 2104
BssECI CCNNGG 5 cut(s) 828, 1347, 1639, 2053, 2454
BssMI GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 2 cut(s) 497, 1667
BssT1I CCWWGG 2 cut(s) 1347, 1639
Bst2BI CACGAG 2 cut(s) 497, 1667
Bst2UI CCWGG 4 cut(s) 1430, 1499, 2455, 2706
Bst4CI ACNGT 6 cut(s) 334, 396, 1240, 1710, 2265, 2518
Bst6I CTCTTC 5 cut(s) 1510, 1855, 1981, 2391, 3065
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 2768
BstBAI YACGTR 1 cut(s) 1943
BstC8I GCNNGC 8 cut(s) 26, 774, 778, 846, 1228, 1413, 1884, 2754
BstF5I GGATG 9 cut(s) 871, 1276, 1294, 1395, 1997, 2074, 2437, 3009, 3112
BstFNI CGCG 1 cut(s) 112
BstHHI GCGC 4 cut(s) 38, 114, 1411, 1417
BstKTI GATC 5 cut(s) 496, 2105, 2788, 2869, 3020
BstMAI GTCTC 4 cut(s) 287, 304, 1419, 2393
BstMBI GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
BstNI CCWGG 4 cut(s) 1430, 1499, 2455, 2706
BstSCI CCNGG 4 cut(s) 1428, 1497, 2453, 2704
BstSFI CTRYAG 8 cut(s) 148, 444, 681, 1118, 1986, 2017, 2530, 2895
BstSLI GKGCMC 1 cut(s) 1647
BstUI CGCG 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 2 cut(s) 2102, 2866
BstXI CCANNNNNNTGG 1 cut(s) 1050
BstYI RGATCY 2 cut(s) 2102, 2866
BsuI GTATCC 2 cut(s) 1207, 1598
BsuRI GGCC 7 cut(s) 106, 188, 529, 1042, 1554, 2241, 2709
BtrI CACGTC 1 cut(s) 1370
BtsCI GGATG 9 cut(s) 871, 1276, 1294, 1395, 1997, 2074, 2437, 3009, 3112
BtsI GCAGTG 4 cut(s) 25, 31, 1237, 1552
BtsIMutI CAGTG 6 cut(s) 25, 31, 325, 1237, 1552, 2312
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 8 cut(s) 26, 774, 778, 846, 1228, 1413, 1884, 2754
CaiI CAGNNNCTG 2 cut(s) 638, 2870
CfoI GCGC 4 cut(s) 38, 114, 1411, 1417
Cfr13I GGNCC 4 cut(s) 105, 1432, 1592, 1683
CseI GACGC 2 cut(s) 221, 524
CsiI ACCWGGT 1 cut(s) 1428
Csp6I GTAC 6 cut(s) 872, 1241, 1804, 2915, 2952, 2985
CviAII CATG 7 cut(s) 563, 971, 1648, 1821, 1874, 2153, 2789
CviQI GTAC 6 cut(s) 872, 1241, 1804, 2915, 2952, 2985
DpnI GATC 5 cut(s) 495, 2104, 2787, 2868, 3019
DpnII GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
DraI TTTAAA 1 cut(s) 472
DraIII CACNNNGTG 1 cut(s) 1733
EaeI YGGCCR 3 cut(s) 1040, 2239, 2707
Eam1104I CTCTTC 5 cut(s) 1510, 1855, 1981, 2391, 3065
EarI CTCTTC 5 cut(s) 1510, 1855, 1981, 2391, 3065
EciI GGCGGA 1 cut(s) 1605
Ecl136II GAGCTC 2 cut(s) 1115, 1895
Eco130I CCWWGG 2 cut(s) 1347, 1639
Eco147I AGGCCT 2 cut(s) 188, 529
Eco24I GRGCYC 4 cut(s) 81, 1117, 1897, 2582
Eco31I GGTCTC 2 cut(s) 1419, 2393
Eco32I GATATC 1 cut(s) 2527
Eco47I GGWCC 3 cut(s) 1432, 1592, 1683
Eco53kI GAGCTC 2 cut(s) 1115, 1895
Eco57I CTGAAG 2 cut(s) 2127, 2271
Eco88I CYCGRG 1 cut(s) 827
EcoICRI GAGCTC 2 cut(s) 1115, 1895
EcoO109I RGGNCCY 1 cut(s) 1683
EcoRII CCWGG 4 cut(s) 1428, 1497, 2453, 2704
EcoRV GATATC 1 cut(s) 2527
EcoT14I CCWWGG 2 cut(s) 1347, 1639
EcoT22I ATGCAT 1 cut(s) 2116
EcoT38I GRGCYC 4 cut(s) 81, 1117, 1897, 2582
ErhI CCWWGG 2 cut(s) 1347, 1639
FaeI CATG 7 cut(s) 566, 974, 1651, 1824, 1877, 2156, 2792
FaqI GGGAC 2 cut(s) 142, 2730
FatI CATG 7 cut(s) 562, 970, 1647, 1820, 1873, 2152, 2788
FauI CCCGC 1 cut(s) 117
FauNDI CATATG 1 cut(s) 2680
FokI GGATG 9 cut(s) 878, 1283, 1301, 1382, 2004, 2081, 2444, 2996, 3119
FriOI GRGCYC 4 cut(s) 81, 1117, 1897, 2582
FspBI CTAG 8 cut(s) 15, 456, 773, 1140, 1766, 1854, 2046, 2610
FspI TGCGCA 1 cut(s) 1410
GlaI GCGC 4 cut(s) 37, 113, 1410, 1416
GsaI CCCAGC 1 cut(s) 2592
GsuI CTGGAG 4 cut(s) 1520, 1784, 2017, 2604
HaeIII GGCC 7 cut(s) 106, 188, 529, 1042, 1554, 2241, 2709
HapII CCGG 4 cut(s) 219, 2242, 2403, 3013
HgaI GACGC 2 cut(s) 221, 524
HhaI GCGC 4 cut(s) 38, 114, 1411, 1417
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 7 cut(s) 566, 974, 1651, 1824, 1877, 2156, 2792
Hin6I GCGC 4 cut(s) 36, 112, 1409, 1415
HinP1I GCGC 4 cut(s) 36, 112, 1409, 1415
HpaII CCGG 4 cut(s) 219, 2242, 2403, 3013
Hpy166II GTNNAC 5 cut(s) 358, 392, 1630, 1806, 2350
Hpy8I GTNNAC 5 cut(s) 358, 392, 1630, 1806, 2350
Hpy99I CGWCG 1 cut(s) 215
HpyCH4III ACNGT 6 cut(s) 334, 396, 1240, 1710, 2265, 2518
HpyCH4IV ACGT 3 cut(s) 1369, 1608, 1942
HpySE526I ACGT 3 cut(s) 1369, 1608, 1942
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 7 cut(s) 566, 974, 1651, 1824, 1877, 2156, 2792
HspAI GCGC 4 cut(s) 36, 112, 1409, 1415
Kpn2I TCCGGA 1 cut(s) 3012
KpnI GGTACC 1 cut(s) 1244
Kzo9I GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
LguI GCTCTTC 2 cut(s) 1981, 3065
LmnI GCTCC 8 cut(s) 221, 430, 979, 1847, 2279, 2585, 2680, 2997
MabI ACCWGGT 1 cut(s) 1428
MaeI CTAG 8 cut(s) 15, 456, 773, 1140, 1766, 1854, 2046, 2610
MaeII ACGT 3 cut(s) 1369, 1608, 1942
MaeIII GTNAC 9 cut(s) 238, 328, 334, 628, 710, 1391, 1723, 2158, 2900
MalI GATC 5 cut(s) 495, 2104, 2787, 2868, 3019
MboI GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
MfeI CAATTG 1 cut(s) 2415
MflI RGATCY 2 cut(s) 2102, 2866
MhlI GDGCHC 9 cut(s) 81, 1117, 1647, 1659, 1852, 1897, 1955, 2016, 2582
MlsI TGGCCA 1 cut(s) 1042
MluNI TGGCCA 1 cut(s) 1042
MlyI GAGTC 3 cut(s) 746, 1336, 2897
MmeI TCCRAC 7 cut(s) 431, 563, 741, 755, 1816, 2003, 2537
Mox20I TGGCCA 1 cut(s) 1042
Mph1103I ATGCAT 1 cut(s) 2116
MroI TCCGGA 1 cut(s) 3012
MroXI GAANNNNTTC 3 cut(s) 554, 1790, 2296
MscI TGGCCA 1 cut(s) 1042
MslI CAYNNNNRTG 5 cut(s) 1001, 1048, 1652, 2730, 2793
Msp20I TGGCCA 1 cut(s) 1042
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 4 cut(s) 219, 2242, 2403, 3013
MspR9I CCNGG 4 cut(s) 1430, 1499, 2455, 2706
MunI CAATTG 1 cut(s) 2415
Mva1269I GAATGC 2 cut(s) 1884, 2881
MvaI CCWGG 4 cut(s) 1430, 1499, 2455, 2706
MvnI CGCG 1 cut(s) 112
NdeI CATATG 1 cut(s) 2680
NdeII GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
NheI GCTAGC 1 cut(s) 772
NlaIII CATG 7 cut(s) 566, 974, 1651, 1824, 1877, 2156, 2792
NlaIV GGNNCC 5 cut(s) 107, 666, 1242, 1594, 2511
NmeAIII GCCGAG 2 cut(s) 2735, 2870
NmuCI GTSAC 5 cut(s) 238, 334, 628, 710, 2900
NsbI TGCGCA 1 cut(s) 1410
NsiI ATGCAT 1 cut(s) 2116
PceI AGGCCT 2 cut(s) 188, 529
PciSI GCTCTTC 2 cut(s) 1981, 3065
PctI GAATGC 2 cut(s) 1884, 2881
PdmI GAANNNNTTC 3 cut(s) 554, 1790, 2296
PfeI GAWTC 8 cut(s) 178, 617, 727, 1067, 1135, 1463, 1791, 2317
PflFI GACNNNGTC 1 cut(s) 1430
PflMI CCANNNNNTGG 1 cut(s) 2519
PleI GAGTC 3 cut(s) 746, 1336, 2897
PpsI GAGTC 3 cut(s) 746, 1336, 2897
Ppu21I YACGTR 1 cut(s) 1943
PpuMI RGGWCCY 1 cut(s) 1683
PshBI ATTAAT 1 cut(s) 3051
Psp124BI GAGCTC 2 cut(s) 1117, 1897
Psp5II RGGWCCY 1 cut(s) 1683
Psp6I CCWGG 4 cut(s) 1428, 1497, 2453, 2704
PspFI CCCAGC 1 cut(s) 2588
PspGI CCWGG 4 cut(s) 1428, 1497, 2453, 2704
PspN4I GGNNCC 5 cut(s) 107, 666, 1242, 1594, 2511
PspPI GGNCC 4 cut(s) 105, 1432, 1592, 1683
PspPPI RGGWCCY 1 cut(s) 1683
PstI CTGCAG 3 cut(s) 152, 448, 1122
PstNI CAGNNNCTG 2 cut(s) 638, 2870
PsuI RGATCY 2 cut(s) 2102, 2866
PsyI GACNNNGTC 1 cut(s) 1430
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 6 cut(s) 873, 1242, 1805, 2916, 2953, 2986
RsaNI GTAC 6 cut(s) 872, 1241, 1804, 2915, 2952, 2985
RseI CAYNNNNRTG 5 cut(s) 1001, 1048, 1652, 2730, 2793
SacI GAGCTC 2 cut(s) 1117, 1897
SapI GCTCTTC 2 cut(s) 1981, 3065
Sau3AI GATC 5 cut(s) 493, 2102, 2785, 2866, 3017
Sau96I GGNCC 4 cut(s) 105, 1432, 1592, 1683
ScaI AGTACT 1 cut(s) 2986
SchI GAGTC 3 cut(s) 746, 1336, 2897
ScrFI CCNGG 4 cut(s) 1430, 1499, 2455, 2706
SduI GDGCHC 9 cut(s) 81, 1117, 1647, 1659, 1852, 1897, 1955, 2016, 2582
SexAI ACCWGGT 1 cut(s) 1428
SfcI CTRYAG 8 cut(s) 148, 444, 681, 1118, 1986, 2017, 2530, 2895
SinI GGWCC 3 cut(s) 1432, 1592, 1683
SmiMI CAYNNNNRTG 5 cut(s) 1001, 1048, 1652, 2730, 2793
SmlI CTYRAG 2 cut(s) 1556, 1620
SmoI CTYRAG 2 cut(s) 1556, 1620
SpeI ACTAGT 1 cut(s) 455
SseBI AGGCCT 2 cut(s) 188, 529
SsiI CCGC 9 cut(s) 110, 216, 227, 230, 1551, 1590, 2078, 2258, 2842
SspI AATATT 4 cut(s) 795, 1480, 1908, 3049
SspMI CTAG 8 cut(s) 15, 456, 773, 1140, 1766, 1854, 2046, 2610
SstI GAGCTC 2 cut(s) 1117, 1897
StuI AGGCCT 2 cut(s) 188, 529
StyD4I CCNGG 4 cut(s) 1428, 1497, 2453, 2704
StyI CCWWGG 2 cut(s) 1347, 1639
TaaI ACNGT 6 cut(s) 334, 396, 1240, 1710, 2265, 2518
TaiI ACGT 3 cut(s) 1372, 1611, 1945
TaqI TCGA 1 cut(s) 1340
TaqII GACCGA 1 cut(s) 224
TatI WGTACW 2 cut(s) 1803, 2984
TauI GCSGC 4 cut(s) 218, 230, 1554, 2844
TfiI GAWTC 8 cut(s) 178, 617, 727, 1067, 1135, 1463, 1791, 2317
TscAI CASTG 6 cut(s) 25, 38, 325, 1237, 1552, 2319
TseFI GTSAC 5 cut(s) 238, 334, 628, 710, 2900
Tsp45I GTSAC 5 cut(s) 238, 334, 628, 710, 2900
TspGWI ACGGA 4 cut(s) 244, 406, 1348, 1855
TspRI CASTG 6 cut(s) 25, 38, 325, 1237, 1552, 2319
Tth111I GACNNNGTC 1 cut(s) 1430
Van91I CCANNNNNTGG 1 cut(s) 2519
VpaK11BI GGWCC 3 cut(s) 1432, 1592, 1683
VspI ATTAAT 1 cut(s) 3051
XapI RAATTY 4 cut(s) 716, 1022, 1401, 2058
XbaI TCTAGA 1 cut(s) 2045
XmnI GAANNNNTTC 3 cut(s) 554, 1790, 2296
XspI CTAG 8 cut(s) 15, 456, 773, 1140, 1766, 1854, 2046, 2610
ZrmI AGTACT 1 cut(s) 2986
Zsp2I ATGCAT 1 cut(s) 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.